Journal of Biomedicine and Biotechnology最新文献_第2页

Structural characterization and antioxidative activity of low-molecular-weights beta-1,3-glucan from the residue of extracted Ganoderma lucidum fruiting bodies. 灵芝子实体低分子量-1,3-葡聚糖的结构表征及抗氧化活性研究。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2011-12-06 DOI: 10.1155/2012/673764

Pai-Feng Kao, Shwu-Huey Wang, Wei-Ting Hung, Yu-Han Liao, Chun-Mao Lin, Wen-Bin Yang

{"title":"Structural characterization and antioxidative activity of low-molecular-weights beta-1,3-glucan from the residue of extracted Ganoderma lucidum fruiting bodies.","authors":"Pai-Feng Kao, Shwu-Huey Wang, Wei-Ting Hung, Yu-Han Liao, Chun-Mao Lin, Wen-Bin Yang","doi":"10.1155/2012/673764","DOIUrl":"https://doi.org/10.1155/2012/673764","url":null,"abstract":"The major cell wall constituent of Ganoderma lucidum (G. lucidum) is β-1,3-glucan. This study examined the polysaccharide from the residues of alkaline-extracted fruiting bodies using high-performance anion-exchange chromatography (HPAEC), and it employed nuclear magnetic resonance (NMR) and mass spectrometry (MS) to confirm the structures. We have successfully isolated low-molecular-weight β-1,3-glucan (LMG), in high yields, from the waste residue of extracted fruiting bodies of G. lucidum. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay evaluated the capability of LMG to suppress H₂O₂-induced cell death in RAW264.7 cells, identifying that LMG protected cells from H₂O₂-induced damage. LMG treatment decreased H₂O₂-induced intracellular reactive oxygen species (ROS) production. LMG also influenced sphingomyelinase (SMase) activity, stimulated by cell death to induce ceramide formation, and then increase cell ROS production. Estimation of the activities of neutral and acid SMases in vitro showed that LMG suppressed the activities of both neutral and acid SMases in a concentration-dependent manner. These results suggest that LMG, a water-soluble β-1,3-glucan recycled from extracted residue of G. lucidum, possesses antioxidant capability against H₂O₂-induced cell death by attenuating intracellular ROS and inhibiting SMase activity.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"673764"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/673764","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30340403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influences of desmin and keratin 19 on passive biomechanical properties of mouse skeletal muscle. 去丝蛋白和角蛋白19对小鼠骨骼肌被动生物力学特性的影响。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-01-04 DOI: 10.1155/2012/704061

Sameer B Shah, James M Love, Andrea O'Neill, Richard M Lovering, Robert J Bloch

{"title":"Influences of desmin and keratin 19 on passive biomechanical properties of mouse skeletal muscle.","authors":"Sameer B Shah, James M Love, Andrea O'Neill, Richard M Lovering, Robert J Bloch","doi":"10.1155/2012/704061","DOIUrl":"https://doi.org/10.1155/2012/704061","url":null,"abstract":"In skeletal muscle fibers, forces must be transmitted between the plasma membrane and the intracellular contractile lattice, and within this lattice between adjacent myofibrils. Based on their prevalence, biomechanical properties and localization, desmin and keratin intermediate filaments (IFs) are likely to participate in structural connectivity and force transmission. We examined the passive load-bearing response of single fibers from the extensor digitorum longus (EDL) muscles of young (3 months) and aged (10 months) wild-type, desmin-null, K19-null, and desmin/K19 double-null mice. Though fibers are more compliant in all mutant genotypes compared to wild-type, the structural response of each genotype is distinct, suggesting multiple mechanisms by which desmin and keratin influence the biomechanical properties of myofibers. This work provides additional insight into the influences of IFs on structure-function relationships in skeletal muscle. It may also have implications for understanding the progression of desminopathies and other IF-related myopathies.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"704061"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/704061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30421330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Synergistic applications of MD and NMR for the study of biological systems. MD和NMR在生物系统研究中的协同应用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-01-26 DOI: 10.1155/2012/254208

Olivier Fisette, Patrick Lagüe, Stéphane Gagné, Sébastien Morin

引用次数: 31

Detection of target ssDNA using a microfabricated Hall magnetometer with correlated optical readout. 使用带有相关光学读出的微制造霍尔磁力计检测目标ssDNA。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-13 DOI: 10.1155/2012/492730

Steven M Hira, Khaled Aledealat, Kan-Sheng Chen, Mark Field, Gerard J Sullivan, P Bryant Chase, Peng Xiong, Stephan von Molnár, Geoffrey F Strouse

{"title":"Detection of target ssDNA using a microfabricated Hall magnetometer with correlated optical readout.","authors":"Steven M Hira, Khaled Aledealat, Kan-Sheng Chen, Mark Field, Gerard J Sullivan, P Bryant Chase, Peng Xiong, Stephan von Molnár, Geoffrey F Strouse","doi":"10.1155/2012/492730","DOIUrl":"https://doi.org/10.1155/2012/492730","url":null,"abstract":"Sensing biological agents at the genomic level, while enhancing the response time for biodetection over commonly used, optics-based techniques such as nucleic acid microarrays or enzyme-linked immunosorbent assays (ELISAs), is an important criterion for new biosensors. Here, we describe the successful detection of a 35-base, single-strand nucleic acid target by Hall-based magnetic transduction as a mimic for pathogenic DNA target detection. The detection platform has low background, large signal amplification following target binding and can discriminate a single, 350 nm superparamagnetic bead labeled with DNA. Detection of the target sequence was demonstrated at 364 pM (<2 target DNA strands per bead) target DNA in the presence of 36 μM nontarget (noncomplementary) DNA (<10 ppm target DNA) using optical microscopy detection on a GaAs Hall mimic. The use of Hall magnetometers as magnetic transduction biosensors holds promise for multiplexing applications that can greatly improve point-of-care (POC) diagnostics and subsequent medical care.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"492730"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/492730","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30569907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Predicting protein interactions by Brownian dynamics simulations. 用布朗动力学模拟预测蛋白质相互作用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-15 DOI: 10.1155/2012/121034

Xuan-Yu Meng, Yu Xu, Hong-Xing Zhang, Mihaly Mezei, Meng Cui

引用次数: 7

Troglitazone induces extracellular matrix and cytoskeleton remodeling in mouse collecting duct cells. 曲格列酮诱导小鼠收集管细胞细胞外基质和细胞骨架重塑。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-03-05 DOI: 10.1155/2012/507057

Jaime Corinaldi, Rania Nasrallah, Jordan Clark, Geneviève Paris, Pedro Miura, Bernard J Jasmin, Richard L Hébert

{"title":"Troglitazone induces extracellular matrix and cytoskeleton remodeling in mouse collecting duct cells.","authors":"Jaime Corinaldi, Rania Nasrallah, Jordan Clark, Geneviève Paris, Pedro Miura, Bernard J Jasmin, Richard L Hébert","doi":"10.1155/2012/507057","DOIUrl":"https://doi.org/10.1155/2012/507057","url":null,"abstract":"Peroxisome proliferator-activated receptor (PPARγ) has been shown to have a protective role in the nephron through its ability to inhibit a transforming growth factor- (TGF-β) mediated fibrotic response. In contrast, PPARγ was also shown to induce a mesenchymal transformation in epithelial intestinal cells. A fibrotic response in the collecting duct has only recently been established; however, the entire collecting duct has not been fully examined. Inner medullary collecting duct cells (IMCD-K2) and mouse cortical collecting duct cells (M1), representing the cortical and medullary collecting duct, were exposed to 5-10 μM troglitazone for 24 hours. Troglitazone resulted in an elongated morphology, 60% decreases in E-cadherin and β-catenin, a 35% decrease in α-catenin, and a 1.5-fold increase in fibronectin. These effects were not reversed with PPARγ antagonists or affected with PPARγ overexpression. Our results indicate that troglitazone induced a mesenchymal-like transformation in M1 and IMCD-K2 epithelial cells independently of PPARγ.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"507057"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/507057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30571518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Role of calcium and mitochondria in MeHg-mediated cytotoxicity. 钙和线粒体在甲基汞介导的细胞毒性中的作用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-07-03 DOI: 10.1155/2012/248764

Daniel Roos, Rodrigo Seeger, Robson Puntel, Nilda Vargas Barbosa

{"title":"Role of calcium and mitochondria in MeHg-mediated cytotoxicity.","authors":"Daniel Roos, Rodrigo Seeger, Robson Puntel, Nilda Vargas Barbosa","doi":"10.1155/2012/248764","DOIUrl":"https://doi.org/10.1155/2012/248764","url":null,"abstract":"Methylmercury (MeHg) mediated cytotoxicity is associated with loss of intracellular calcium (Ca²⁺) homeostasis. The imbalance in Ca²⁺ physiology is believed to be associated with dysregulation of Ca²⁺ intracellular stores and/or increased permeability of the biomembranes to this ion. In this paper we summarize the contribution of glutamate dyshomeostasis in intracellular Ca²⁺ overload and highlight the mitochondrial dysfunctions induced by MeHg via Ca²⁺ overload. Mitochondrial disturbances elicited by Ca²⁺ may involve several molecular events (i.e., alterations in the activity of the mitochondrial electron transport chain complexes, mitochondrial proton gradient dissipation, mitochondrial permeability transition pore (MPTP) opening, thiol depletion, failure of energy metabolism, reactive oxygen species overproduction) that could culminate in cell death. Here we will focus on the role of oxidative stress in these phenomena. Additionally, possible antioxidant therapies that could be effective in the treatment of MeHg intoxication are briefly discussed.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"248764"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/248764","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30863273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Animal experimental study of the fully biodegradable atrial septal defect (ASD) occluder. 全生物可降解房间隔缺损封堵器的动物实验研究。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-02 DOI: 10.1155/2012/735989

Yu-feng Zhu, Xin-miao Huang, Jiang Cao, Jian-qiang Hu, Yuan Bai, Hai-bing Jiang, Zhao-feng Li, Ying Chen, Wei Wang, Yong-wen Qin, Xian-xian Zhao

{"title":"Animal experimental study of the fully biodegradable atrial septal defect (ASD) occluder.","authors":"Yu-feng Zhu, Xin-miao Huang, Jiang Cao, Jian-qiang Hu, Yuan Bai, Hai-bing Jiang, Zhao-feng Li, Ying Chen, Wei Wang, Yong-wen Qin, Xian-xian Zhao","doi":"10.1155/2012/735989","DOIUrl":"https://doi.org/10.1155/2012/735989","url":null,"abstract":"This study was conducted to evaluate the feasibility, safety, biocompatibility, and degradation features of a fully biodegradable occluder for closure of atrial septal defect (ASD) in an acute canine model. The ASD was created in 20 healthy mongrel dogs by the brockenbrough needle, and the fully biodegradable occluders were implanted by self-made delivery system. The success rate and complications were observed. Acute ASD models were successfully created in 18 dogs, and 16 occluders were successfully implanted in the ASD models. Animals were sacrificed at different times after procedure. The cardiac gross anatomy showed that all occluders were stable in the interatrial septum, no vegetation or thrombus formation was observed on the surface of all occluders. They were embedded into endogenous host tissue gradually at 12-week follow-up. Different periods of pathological observations suggested that the occluders degraded gradually over about 24 weeks and essentially became an integral part of the septum. Transcatheter closure of ASD in acute canine model using the fully biodegradable ASD occluder has the potential of a high successful rate of technique, excellent biocompatibility, and fewer complications with adequate, immediate, and short-term results.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"735989"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/735989","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30998261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Protein palmitoylation and pathogenesis in apicomplexan parasites. 顶复合体寄生虫的蛋白棕榈酰化及其发病机制。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-03 DOI: 10.1155/2012/483969

Maria Martha Corvi, Andres Mariano Alonso, Marina Cecilia Caballero

引用次数: 12

Acanthus ebracteatus Vahl. ethanol extract enhancement of the efficacy of the collagen scaffold in wound closure: a study in a full-thickness-wound mouse model. 棘棘;乙醇提取物增强胶原支架在伤口愈合中的作用:全层伤口小鼠模型的研究。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-02 DOI: 10.1155/2012/754527

Jutamas Somchaichana, Tanom Bunaprasert, Suthiluk Patumraj

{"title":"Acanthus ebracteatus Vahl. ethanol extract enhancement of the efficacy of the collagen scaffold in wound closure: a study in a full-thickness-wound mouse model.","authors":"Jutamas Somchaichana, Tanom Bunaprasert, Suthiluk Patumraj","doi":"10.1155/2012/754527","DOIUrl":"https://doi.org/10.1155/2012/754527","url":null,"abstract":"Acanthus ebracteatus Vahl. is a Thai herb that is effective in wound healing. We sought to quantitatively determine whether or not the combined application of Acanthus ebracteatus Vahl. and a collagen scaffold will increase wound closure and angiogenesis. Balb/c mice (body weight: 22-25 g) were anesthetized with sodium thiopental. The dorsal skin incision measuring 1.5 × 1.5 cm was made and then deepened using scissors to produce a full-thickness incision down to the level of the panniculus carnosus. The size of the wound was approximately 10% of the total body surface area. The collagen sheet was implanted onto the wound. Animals were divided into 4 major groups as follows: wound with normal saline (W-NSS), wound treated with 0.3 g/kg BW of Acanthus ebracteatus Vahl. extract (W-AE (0.3 g/kg.bw)), wound implanted with collagen scaffold (W-Coll), and wound implanted with collagen scaffold and treated with 0.3 g/kg BW of Acanthus ebracteatus Vahl. (W-Coll-AE combination). On day 14, the W-Coll-AE group showed decreased wound areas and increased capillary vascularity (CV) when compared to the other 3 groups, W-NSS, W-AE0.3, and W-Coll. In the present study, the combination of AE0.3 with collagen showed the best effect on skin angiogenesis and promoted wound closure with less neutrophil infiltration.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"754527"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/754527","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31000309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22