Journal of Asthma最新文献

{"title":"Establishment of a multimodal respiratory function diagnostic model and its role in the differential diagnosis of bronchial asthma and chronic obstructive pulmonary disease with incomplete reversible airflow limitation.","authors":"Peng Li, Li Sun, Chao Wang, Qingzhen Gao","doi":"10.1080/02770903.2026.2701399","DOIUrl":"10.1080/02770903.2026.2701399","url":null,"abstract":"<p><strong>Objective: </strong>This retrospective diagnostic test aimed to explore a method that can differentiate asthma from COPD with incomplete reversible airflow limitation.</p><p><strong>Methods: </strong>Patients diagnosed with asthma and COPD from June 19, 2024, to November 16, 2025, were enrolled as training samples. Intergroup data were analyzed through discriminant analysis to determine the quantitative diagnostic formula and the discriminant boundary value <i>Z_c</i> that could identify them. A portion of the patients collected in the same way were used as validation samples to verify the accuracy of the model.</p><p><strong>Results: </strong>128 patients with asthma and 142 with COPD were enrolled as training samples. Through discriminant analysis, the quantitative diagnostic formula was <i>Zi</i> = 1.476<i>X</i>₁+0.348<i>X</i>₂-0.269<i>X</i>₃-0.465<i>X</i>₄+0.995<i>X</i>₅+0.046<i>X</i>₆+0.013<i>X</i>₇-4.479 (<i>X</i>₁ = 0 and <i>X</i>₁ = 1 represent the absence or presence of the concave pattern in the F-V, respectively; <i>X</i>₂, <i>X</i>₃ and <i>X</i>₄ represent forced vital capacity, forced expiratory volume in 1s, and peak expiratory flow, as indicators of bronchodilation test, respectively, 0 = negative, 1 = positive; <i>X</i>₅ represents gender, <i>X</i>₅ = 0 indicates female, and <i>X</i>₅ = 1 indicates male; <i>X</i>₆ represents age; <i>X</i>₇ represents smoking index), and <i>Z</i>_c = -0.0687. The accuracy of the quantitative diagnostic formula, validated internally and externally using 121 patients, was 90.40% and 87.60%, respectively.</p><p><strong>Conclusions: </strong>The multimodal respiratory function diagnostic model, utilizing discriminant analysis, can distinguish asthma with incomplete reversible airflow limitation from COPD when the strongest expiratory effort is performed with an extrapolated volume ≤0.10 L.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1670-1679"},"PeriodicalIF":1.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic model construction of mitochondrial-mitophagy related genes and their regulatory network and potential drug discovery in childhood allergic asthma.","authors":"Wei Lei, Rui Qu, Ning Wang","doi":"10.1080/02770903.2026.2696578","DOIUrl":"10.1080/02770903.2026.2696578","url":null,"abstract":"<p><p><b>Objective:</b> Childhood allergic asthma (CAA) is a chronic allergen-driven airway inflammatory disease whose molecular mechanisms and precise diagnostic and therapeutic strategies remain incompletely understood. This study aimed to identify mitochondrial-mitophagy-related genes (MMRGs) with diagnostic and therapeutic potential in CAA.<b>Methods:</b> Public transcriptomic datasets were analyzed using weighted gene co-expression network analysis and multiple machine learning algorithms to identify diagnostic signature genes and construct a CAA diagnostic model. Immune infiltration, competing endogenous RNA network, and drug-gene interaction analyses were subsequently performed. The expression and biological functions of selected genes were further evaluated in an IL-13-stimulated airway epithelial cell model.<b>Results:</b> Fifteen MMRG-related signature genes with diagnostic potential were identified, and the resulting diagnostic model exhibited good predictive performance. Several immune cell subsets, including resting mast cells, and naive B cells, showed altered infiltration in CAA, and their abundances were significantly correlated with signature-gene expression. The constructed ceRNA network indicated complex post-transcriptional regulation of these genes. Drug-gene interaction analysis identified multiple potential therapeutic compounds, with BCL2-related agents being the most abundant. In IL-13-stimulated airway epithelial cells, MRPL43 expression was increased, whereas BCL2 expression was decreased. MRPL43 knockdown significantly restored cell viability and reduced the secretion of pro-inflammatory cytokines.<b>Conclusions:</b> MMRGs are closely associated with the diagnosis, immune microenvironment, and potential therapeutic regulation of CAA. MRPL43 may contribute to airway epithelial inflammation and represents a potential biomarker and therapeutic target for CAA.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1566-1582"},"PeriodicalIF":1.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148375916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhaled corticosteroids monotherapy in childhood asthma: Any differential response rate between activated and prodrugs?","authors":"Poly Majumder, Anna Smyrnova, Francine M Ducharme","doi":"10.1080/02770903.2026.2686931","DOIUrl":"10.1080/02770903.2026.2686931","url":null,"abstract":"<p><strong>Objective: </strong>The efficacy of inhaled corticosteroids (ICS) as prodrugs (inactive at delivery) has been compared to activated ICS, in mean changes in lung function and symptoms, with limited evidence on achieving control. We aimed to compare the real-life success rate of two broad ICS types (prodrug and activated) to prevent poor asthma control and exacerbations in children with asthma.</p><p><strong>Methods: </strong>We conducted a retrospective cohort study of children aged 1-17 years, initiating a new maintenance ICS monotherapy at low or medium doses. Initiation referred to a new ICS (after ≥2-month washout) or a switch to another ICS type (prodrug or activated). Co-primary outcomes included: unsatisfactory asthma control, measured by the Pharmacoepidemiologic Pediatric Asthma Control Index, and occurrence of ≥1 severe exacerbation, in following 6 months. A propensity score (PS) weight-adjusted binary logistic regression compared outcomes between ICS types, reporting odds ratios [95% confidence intervals].</p><p><strong>Results: </strong>Of 213 participants (56% male, median age: 6.9 years), 194 were incident users and 19, switchers. Most (<i>N</i> = 148) children were prescribed an activated ICS (97.3% fluticasone propionate, 2.7% budesonide) and 65, an ICS prodrug (100% ciclesonide), 61% using low-dose ICS. The PS-adjusted odds of unsatisfactory asthma control were significantly higher in the prodrug than activated ICS groups (adjOR: 2.35 [95% CI: 1.46-3.81]), with no significant group difference in severe exacerbations (adjOR: 0.92 [95% CI: 0.49-1.72]).</p><p><strong>Conclusions: </strong>Compared to activated ICS, children initiating a prodrug were two-fold more likely to display unsatisfactory control, with no significant difference nor equivalence in likelihood of severe exacerbations.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1479-1487"},"PeriodicalIF":1.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148239404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Translational models for acute asthma: critical evaluation of in vivo, in vitro, and emerging technologies in drug discovery\".","authors":"Raihana Nilofar A, Rajendran Priyadharsini","doi":"10.1080/02770903.2026.2721179","DOIUrl":"10.1080/02770903.2026.2721179","url":null,"abstract":"<p><strong>Objectives: </strong>To critically evaluate conventional and emerging preclinical methodologies for screening novel asthma therapeutics; to compare their translational relevance; and to propose an integrated framework aimed at improving precision, safety, and phenotype-targeted efficacy in drug development.</p><p><strong>Data sources: </strong>A review of peer-reviewed literature was conducted using PubMed. Sources included studies on enzymatic and receptor-binding assays, <i>in vitro</i> cellular platforms, allergen-induced animal models, and recent innovations such as 3D airway organoids, lung-on-a-chip microfluidics, and omics-guided biomarker discovery.</p><p><strong>Study selections: </strong>Included studies focused on (i) molecular validation of drug targets, (ii) mechanistic and high-throughput screening in cellular systems, (iii) <i>in vivo</i> replication of asthma pathophysiology in animal models, or (iv) technologies enhancing preclinical predictability.</p><p><strong>Results: </strong>Conventional screening assays provide molecular validation but lack complexity. <i>In vitro</i> systems allow mechanistic insight and scalability, yet do not fully reproduce airway interactions. Allergen-induced animal models replicate inflammation and hyperresponsiveness but fail to capture human heterogeneity or acute exacerbations, a major contributor to morbidity and mortality. Acute asthma remains particularly difficult to model, limiting evaluation of therapies aimed at severe attacks. Emerging approaches, including organoids, lung-on-a-chip, and omics-based biomarkers, offer improved physiological relevance, dynamic assessment of inflammatory responses, and greater potential to model acute disease states.</p><p><strong>Conclusions: </strong>Integrating conventional and innovative models may accelerate the development of safer, more effective, and phenotype-specific asthma therapies. Approaches that incorporate both chronic disease and acute exacerbations could reduce steroid resistance, improve emergency care, and enhance translational success across diverse populations.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1451-1467"},"PeriodicalIF":1.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison between three digital peak expiratory flow devices and spirometry in children and adolescents with asthma.","authors":"Paula do Nascimento Maia, Ana Alice Amaral Ibiapina Parente, Maria de Fátima Bazhuni Pombo Sant Anna, Rafaela Baroni Aurilio, Ronir Raggio Luiz, Raquel Tavares Borda Garrido, Clemax Couto Sant Anna","doi":"10.1080/02770903.2026.2696581","DOIUrl":"10.1080/02770903.2026.2696581","url":null,"abstract":"<p><p>Objective: We compared lung function measurements of three digital peak flow meters (DPMs) to those from spirometry pre and post-bronchodilation (BD). Methods: We conducted a cross-sectional analysis in patients with asthma between 6 and 18 years, evaluating three DPMs: Microlife, Piko, and Asthma-1. We assessed the agreement between measurements using paired t-tests, intraclass correlation coefficients (ICCs), Bland–Altman plots, and survival–agreement plots. Results: The ICCs for peak expiratory flow (PEF) were 0.756 (pre-BD) and 0.762 (post-BD). The spirometry mean was 3.63 L/s, and differences were observed for Microlife (−0.13; p = 0.025), Piko (−0.36; p < 0.001), and Asthma-1 (−0.38; p < 0.001); with similar trends in post-BD. The pre-BD spirometric mean was 1.83 L for forced expiratory volume in 1 s (FEV₁), with significant difference for Microlife (−0.14), Piko (−0.21), and Asthma-1 (−0.30) (all p < 0.001). The corresponding pre-BD ICC was 0.762, with similar results observed post-BD. Bland–Altman analysis for PEF revealed pre-BD differences for Microlife (+2 to −3), Piko (+5 to −4), and Asthma-1 (+2 to −3). Post-BD results showed no change for Microlife and Piko. However, for Asthma-1, a difference of +2 to −4 was observed. FEV₁, differences for Microlife ranged from +0.75 to −1.5 (pre-BD) and +0.5 to −1.5 (post-BD) were observed. Piko ranges were +5.5 to −1.5 (pre-BD) and +4.5 to −2.5 (post-BD); Asthma-1 ranges were +0.5 to −1.5 (pre-BD) and +0.25 to −1.75 (post-BD). Similar results were obtained using the survival–agreement. Conclusions: All DPMs showed moderate agreement with spirometry, with the Microlife device demonstrated comparatively greater accuracy.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1602-1610"},"PeriodicalIF":1.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148352048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global research on pulmonary diseases and environmental factors: a bibliometric and knowledge-mapping study.","authors":"Peng-Fei Guo, XiaoMei Li, Jie Wang, Jianjun Fan, Hai-Long Zhang, Ning Su, Yi-Chi Zhang, Ming Zhang, Chu-Qing Wang, Yun-Fei Gao, Zhi-Ying Zhao","doi":"10.1080/02770903.2026.2696564","DOIUrl":"10.1080/02770903.2026.2696564","url":null,"abstract":"<p><strong>Objective: </strong>Research on pulmonary diseases and environmental factors has expanded rapidly, yet the field remains fragmented across exposure categories, disease outcomes and disciplinary boundaries, and its overall knowledge structure has not been systematically characterized.</p><p><strong>Methods: </strong>We conducted a bibliometric and knowledge-mapping study using 505 articles from the Web of Science Core Collection (WOSCC) and 489 records from PubMed, all retrieved on May 14, 2025. WOSCC was used to analyze publication trends, disciplinary structure, collaboration networks, thematic hotspots, co-citation patterns and funding profiles, whereas PubMed was used to assess country- and region-level productivity and international collaboration.</p><p><strong>Results: </strong>Publication output increased markedly after 2012 and remained consistently active from 2020 to 2024, while records indexed in 2025 were treated as partial-year data. The field was shaped primarily by Environmental Sciences, Public, Environmental and Occupational Health, and Toxicology, with relatively sparse collaboration networks and output concentrated in a limited number of authors, institutions and countries. Thematic analyses showed a clear evolution from broad exposure-disease associations toward more differentiated research on particulate matter, air quality, susceptibility, prevention and biological mechanisms. In particular, oxidative stress emerged as a key mechanistic hub linking environmental exposures with pulmonary injury and adverse respiratory outcomes. The intellectual base of the field was anchored in particulate pollution, inhalational exposure events and pulmonary function assessment.</p><p><strong>Conclusion: </strong>Research on pulmonary diseases and environmental factors has developed into a stable but still fragmented interdisciplinary domain, with current trends indicating a shift from descriptive association studies toward mechanism-oriented and prevention-relevant research.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1418-1434"},"PeriodicalIF":1.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between dietary intake of live microbes and asthma risk in children and adolescents aged 6-15 years: a cross-sectional analysis.","authors":"Kaishun Zhao, Ran Dong, Kena Bao, Wei Wei, Yanfang Yu","doi":"10.1080/02770903.2026.2701395","DOIUrl":"10.1080/02770903.2026.2701395","url":null,"abstract":"<p><p>Emerging evidence implicates gut dysbiosis in asthma pathogenesis via the gut-lung axis. This cross-sectional study aimed to examine the associations between dietary live microbe intake and asthma risk among US children and adolescents aged 6-15 years.</p><p><strong>Methods: </strong>We analyzed data from the 2007-2018 National Health and Nutrition Examination Survey. Dietary live microbe intake was evaluated using the Sanders method and categorized into low, medium, and high categories. Asthma status was determined by self-report. Multivariable binary logistic regression was used to evaluate the association between dietary live microbe intake and asthma prevalence.</p><p><strong>Results: </strong>Of the 7547 participants (mean age 10.8 years; 51.9% male) included, the overall prevalence of asthma was 11.3%. Compared with the low dietary live microbes intake group, the multivariate-adjusted odds ratios (95% confidence intervals) for asthma in the medium and high intake groups were 0.75 (0.63-0.88) and 0.52 (0.41-0.64), respectively (<i>p</i> for trend < 0.001). Subgroup analyses suggested trends toward stronger protective associations among girls (<i>p</i> for interaction = 0.073) and those exposed to household smoking (<i>p</i> for interaction = 0.070). Sensitivity analyses using propensity score matching and additional adjustment for prebiotics, probiotics, and synbiotics yielded results consistent with the primary analysis.</p><p><strong>Conclusions: </strong>Higher dietary live microbe intake was significantly associated with lower asthma prevalence in US children and adolescents aged 6-15 years. These findings support additional investigation of dietary live microbe consumption as a modifiable factor for asthma prevention in this age group.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1650-1658"},"PeriodicalIF":1.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Negative association between asthma and tuberculosis: the mediating effect of albumin.","authors":"Ling-Yan Chen, Hai-Bo Hua, Yuan-Yuan Chen","doi":"10.1080/02770903.2026.2701401","DOIUrl":"10.1080/02770903.2026.2701401","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the relationship between asthma and tuberculosis (TB) risk and to explore whether oxidative stress-related serum markers available in National Health and Nutrition Examination Survey (NHANES) mediate this association.</p><p><strong>Methods: </strong>Data were obtained from NHANES. Multivariate logistic regression was used to examine the association between asthma and TB and between oxidative stress-related serum markers and TB. Generalized linear regression was used to analyze the association between asthma and oxidative stress-related serum markers. Mediation analysis was used to evaluate the role of albumin, as the only marker that remained significant, in the association between asthma and TB. A core propensity score matching (PSM) sensitivity analysis was performed.</p><p><strong>Results: </strong>Asthma was associated with TB risk in 3 different models (all odds ration [OR] < 1, all <i>p</i> < 0.05). Among oxidative stress indicators, only albumin concentration was associated with TB risk, and higher albumin concentrations were associated with lower TB risk (all <i>p</i> < 0.05). Furthermore, asthma was associated with albumin concentration in 3 models (all <i>β</i> < 0, all <i>p</i> < 0.05). Finally, albumin was identified as a mediator in the association between asthma and TB risk. In a core PSM-matched sample, the inverse asthma-TB association remained significant in the final model, whereas the matched-sample albumin-TB and indirect mediation estimates were attenuated.</p><p><strong>Conclusion: </strong>Asthma was negatively associated with TB risk, and albumin, among the evaluated oxidative stress-related serum markers, appeared to exert a significant mediating effect on this association. This direction was generally preserved in the matched sensitivity analysis.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1680-1687"},"PeriodicalIF":1.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perceptions, attitudes, and real-world use of FENO in allergy and asthma practice: Results of a national survey from türkiye.","authors":"Betül Özdel Öztürk, Zeynep Şengül Emeksiz, Zeynep Çelebi Sözener, Özge Yılmaz, Ersoy Civelek, Sevim Bavbek","doi":"10.1080/02770903.2026.2701397","DOIUrl":"10.1080/02770903.2026.2701397","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate real-life use, perceived clinical value, and barriers to the implementation of fractional exhaled nitric oxide (FeNO) among allergy-immunology specialists in Türkiye.</p><p><strong>Methods: </strong>This nationwide online survey, developed by the Asthma Working Group of the Turkish National Society of Allergy and Clinical Immunology, included 35 multiple-choice questions administered to pediatric and adult allergy-immunology specialists and fellows after the 30th National Congress.</p><p><strong>Results: </strong>Of the 200 respondents, 75% (<i>n</i> = 150) were female, 54% (<i>n</i> = 108) were early-career physicians, and the majority (<i>n</i> = 125, 62.5%) worked in a university or training and research hospital. Asthma was identified as the primary indication for FeNO measurement by 95% (<i>n</i> = 190), of participants. FeNO was most frequently perceived as useful for phenotyping patients before biologic therapy initiation (<i>n</i> = 150,75.0%), monitoring treatment response (<i>n</i> = 141,70.5%), assessing asthma severity (<i>n</i> = 105, 52.5%), and supporting diagnosis (<i>n</i> = 85, 42.5%). In asthma management, FeNO was considered valuable for guiding decisions before biologic therapy (<i>n</i> = 173, 86.5%), assessing treatment adherence (<i>n</i> = 147, 73.5%), estimating exacerbation risk (<i>n</i> = 134, 67.0%), and adjusting inhaled corticosteroid doses (<i>n</i> = 119, 59.5%). Nearly all participants (<i>n</i> = 195, 97.5%) agreed that FeNO should be used as a complementary tool. However, only 7.0% (<i>n</i> = 14) reported routine FeNO use in daily practice. Limited device access, high cost, lack of reimbursement, and insufficient training were identified as major barriers. Knowledge gaps were prominent, with 61% reporting little or no practical knowledge of FeNO.</p><p><strong>Conclusion: </strong>This first national survey demonstrates a marked gap between clinicians' recognition of FeNO's clinical value and its limited real-world use in Türkiye, largely due to cost, access, and educational barriers.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1659-1669"},"PeriodicalIF":1.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148404821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance and effects of miR-431-5p in children with asthma.","authors":"Yafei Hou, Luping Ji, Jielin Gao","doi":"10.1080/02770903.2026.2696584","DOIUrl":"10.1080/02770903.2026.2696584","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the clinical significance of miR-431-5p in childhood asthma and its cell function in house dust mite (HDM)-induced cell models.</p><p><strong>Methods: </strong>Differential miRNA expression was analyzed in the GSE263408 dataset using bioinformatics tools (Limma package). Serum levels of miR-431-5p and CD34 mRNA in asthmatic and healthy children were measured. The diagnostic and prognostic potentials of miR-431-5p were evaluated using ROC curve analysis. The targeting relationship between miR-431-5p and CD34 was predicted by TargetScan and validated by dual-luciferase reporter assay. <i>In vitro</i>, transfected with miR-431-5p mimic or CD34 pcDNA, human airway smooth muscle cells (ASMCs) and mast cells were treated with HDM to assess effects on RNA expression, ASMC cell viability, and mast cell degranulation/histamine content.</p><p><strong>Results: </strong>Bioinformatic analysis identified miR-431-5p as differentially expressed in asthma. Serum miR-431-5p was significantly downregulated in asthmatic children compared to healthy controls, showing diagnostic value (AUC = 0.866). It was further decreased in children with asthma recurrence/exacerbation, with an AUC of 0.787 in discriminating recurrence/exacerbation from none. miR-431-5p targeted CD34 3'UTR. In HDM-induced ASMCs, miR-431-5p mimic reduced CD34, MUC5AC mRNA, and cell viability, which were rescued by CD34 overexpression. In HDM-induced mast cells, miR-431-5p mimic reduced CD34 mRNA, degranulation, and histamine content, effects also reversed by CD34 overexpression.</p><p><strong>Conclusions: </strong>Serum miR-431-5p has potential as a biomarker for childhood asthma. miR-431-5p may mitigate asthma-related cellular processes by targeting CD34.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1628-1637"},"PeriodicalIF":1.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148375856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}