Journal of Biological Rhythms最新文献

{"title":"Clock-Dependent Phosphorylation of CikA Regulates Its Activity.","authors":"Cigdem Sancar, Susan S Golden","doi":"10.1177/07487304251338156","DOIUrl":"10.1177/07487304251338156","url":null,"abstract":"<p><p>In the cyanobacterial circadian clock, a core oscillator comprising the proteins KaiA, KaiB, and KaiC keeps time based on a rhythmic phosphorylation of KaiC, and histidine protein kinases relay temporal information from the KaiABC complex to regulate gene expression. The kinases SasA and CikA engage directly with the oscillator and are responsible for modulating the phosphorylation and dephosphorylation throughout the circadian day of the response-regulator transcription factor RpaA; the phosphorylation state of RpaA in turn determines circadian gene expression. We recently showed that either CikA or SasA can drive rhythmic phosphorylation and DNA binding of RpaA in an in vitro system. However, when SasA is absent in vivo, a bioluminescence reporter gene shows a very low expression and amplitude rhythm, indicating CikA kinase activity is not sufficient to activate gene expression. We questioned why CikA cannot serve as a robust kinase for RpaA in the absence of SasA in the cell. Here, we investigated post-translational modifications of CikA and found KaiC-dependent phosphorylation sites of CikA that dramatically affect its activity. Phosphomimetic mutants of these sites showed that the phosphorylated version of CikA is not functional. Our data show that inverse correlation of KaiC levels and these inhibitory phosphorylation sites can explain the lower CikA activity in a SasA knockout background. We conclude that these phosphorylation sites act as a rheostat for CikA activity and are regulated by KaiC levels.</p>","PeriodicalId":15056,"journal":{"name":"Journal of Biological Rhythms","volume":" ","pages":"455-467"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12353112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal Associations of Sedentary Behavior and Physical Activity With Diurnal Rest-Activity Rhythms in Survivors of Colorectal Cancer Up to 5 Years Post-Treatment.","authors":"Koen G Frenken, Marvin Y Chong, Stéphanie O Breukink, Maryska Janssen-Heijnen, Eric T P Keulen, Joop Konsten, Wouter Bijnens, Laurien M Buffart, Kenneth Meijer, Frank A J L Scheer, Karen Steindorf, Judith de Vos-Geelen, Matty P Weijenberg, Martijn J L Bours, Eline H van Roekel","doi":"10.1177/07487304251363674","DOIUrl":"https://doi.org/10.1177/07487304251363674","url":null,"abstract":"<p><p>Disrupted diurnal rest-activity rhythms (RAR), that is, daily 24-h patterns of rest and activity, have been associated with fatigue and decreased quality of life among survivors of colorectal cancer (CRC). To identify potential targets for interventions to improve RAR, we investigated longitudinal associations of time spent in sedentary behavior and physical activity with RAR parameters after CRC treatment. In a prospective cohort study, repeated measurements were performed among 268 survivors of stage I-III CRC at 6 weeks, 6 months, and 1, 2, and 5 years after treatment. Thigh-worn accelerometers were used to determine hours/day spent in sedentary behavior, standing, and total physical activity during waking time, as well as RAR parameters including mesor, amplitude, circadian quotient (CQ), dichotomy index (I < O) and 24 h-autocorrelation (R24). Self-reported light-intensity physical activity (LPA) and moderate-to-vigorous physical activity (MVPA) were determined via the validated SQUASH questionnaire. Longitudinal associations were analyzed using confounder-adjusted linear mixed models. More sedentary time was statistically significantly associated with a lower mesor, amplitude, I < O and R24 over the 5-year post-treatment period. More standing time was associated with a higher mesor, amplitude, CQ, and I < O but not with R24. Higher levels of objectively assessed total physical activity as well as self-reported MVPA were associated with higher values for all RAR parameters. LPA was not associated with any of the RAR parameters. In the years after CRC treatment, less sedentary behavior and more standing and physical activity were generally associated with higher RAR parameters indicating a more robust rhythm. Future studies should provide more insight into causality of these associations as RAR may be a potential new target for interventions to reduce fatigue after CRC.Trial registration: EnCoRe study NL6904 (https://www.Onderzoekmetmensen.nl/).</p>","PeriodicalId":15056,"journal":{"name":"Journal of Biological Rhythms","volume":" ","pages":"7487304251363674"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"It's Not Just Light: Assessing the Intrinsic Period of the Human Circadian Pacemaker Under Dim Light Conditions Should Include Non-Photic Influences.","authors":"Melissa A St Hilaire, Charles A Czeisler, Elizabeth B Klerman","doi":"10.1177/07487304251355451","DOIUrl":"10.1177/07487304251355451","url":null,"abstract":"<p><p>Response to: \"Mathematical Analysis of Light-sensitivity Related Challenges in Assessment of the Intrinsic Period of the Human Circadian Pacemaker\".</p>","PeriodicalId":15056,"journal":{"name":"Journal of Biological Rhythms","volume":" ","pages":"414-420"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How to Deal With Darkness: Modeling and Visualization of Zero-Inflated Personal Light Exposure Data on a Logarithmic Scale.","authors":"Johannes Zauner, Carolina Guidolin, Manuel Spitschan","doi":"10.1177/07487304251336624","DOIUrl":"10.1177/07487304251336624","url":null,"abstract":"<p><p>Measuring and analyzing personal light exposure has become increasingly important in circadian and myopia research. Very small measurement values in light exposure patterns, especially zero, are regularly recorded in field studies. These zero-lux values are problematic for commonly applied logarithmic transformations and should neither be dismissed nor be unduly influential in visualizations and statistical modeling. We compare 4 ways to visualize such data on a linear, logarithmic, hybrid, or symlog scale, and we model the light exposure patterns with a generalized additive model by removing zero-lux values, adding a very small or -1 log₁₀ lux value to the dataset, or using the Tweedie error distribution. We show that a <i>symlog</i>-transformed visualization, implemented in <i>LightLogR</i>, displays relevant features of light exposure across scales, including zero-lux, while reducing the emphasis on the small values (<1 lux). <i>Symlog</i> is well-suited to visualize differences in light exposure covering heavy-tailed negative values. We further show that small but not negligible value additions to the light exposure data of -1 log₁₀ lux for statistical modeling allow for acceptable models on a logarithmic scale, while very small values distort results. We also demonstrate the utility of the Tweedie distribution, which does not require prior transformations, models data on a logarithmic scale, and includes zero-lux values, capturing personal light exposure patterns satisfactorily. Data from field studies of personal light exposure require appropriate handling of zero-lux values in a logarithmic context. <i>Symlog</i> scales for visualizations and an appropriate addition to input values for modeling, or the Tweedie distribution, provide a solid basis. Beyond light exposure, other time-series data relevant to biological rhythms, such as accelerometry for ambulatory sleep scoring in humans or wheel-running in animal models, exhibit zero inflation and can benefit from the methods introduced here.</p>","PeriodicalId":15056,"journal":{"name":"Journal of Biological Rhythms","volume":" ","pages":"480-490"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated Batch Processing of Diurnal Cardiac Activity: Comparison of Fully Automated Batch- to Gold-Standard Manual Processing.","authors":"Maximilian Schmausser, Christoph Hoog Antink, Michael Kaess, Julian Koenig","doi":"10.1177/07487304251348516","DOIUrl":"10.1177/07487304251348516","url":null,"abstract":"<p><p>The analysis of long-term variation patterns in heart rate (HR) and heart rate variability (HRV) provides insights into autonomic nervous system function beyond short-term recordings taken under resting or experimental conditions. Yet, traditional processing pipelines often require time- and labor-intensive visual inspection of electrocardiography (ECG) data and manual artifact removal. This study evaluated the performance of 3 code-based fully automated batch-processing pipelines-<i>NeuroKit2</i>, <i>RHRV</i>, and <i>Systole</i>-against the manual gold standard utilizing Kubios for both (diurnal) HR and HRV estimates derived from raw 48-h ECG recordings. Results illustrate that while automated pipelines yield HR estimates in good agreement to the gold standard (<i>r</i> = 0.91-0.99; α = 0.90-0.99), HRV estimates exhibit greater deviations (<i>r</i> = 0.66-0.87; α = 0.76-0.90). Cosinor analyses of diurnal HR patterns indicate strong consistency between Kubios and NeuroKit2 (<i>r</i> = 0.94-0.99; α = 0.97-0.99), but weaker correlations with RHRV and Systole (<i>r</i> = 0.58-0.87; α = 0.63-0.93). HRV cosinor parameters showed even larger discrepancies, with parameter-dependent correlations ranging from <i>r</i> = 0.41 to 0.86 and Cronbach's alphas from α = 0.59 to 0.91. Findings suggest that automated batch processing of ECG data for analyzing diurnal variation patterns in HR and HRV produces results that show moderate to good agreement with the gold standard including visual inspection and manual processing. However, caution is warranted, as existing toolboxes and pipelines may lead to different results.</p>","PeriodicalId":15056,"journal":{"name":"Journal of Biological Rhythms","volume":" ","pages":"468-479"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence for Internal Misalignment of Circadian Rhythms in Youth With Emerging Mood Disorders.","authors":"Joanne S Carpenter, Jacob J Crouse, Mirim Shin, Emiliana Tonini, Gabrielle Hindmarsh, Zsofi de Haan, Frank Iorfino, Rebecca Robillard, Sharon Naismith, Elizabeth M Scott, Ian B Hickie","doi":"10.1177/07487304251349408","DOIUrl":"10.1177/07487304251349408","url":null,"abstract":"<p><p>Despite evidence for links between circadian dysfunction and mood disorders, previous research has largely reported on single biological markers of circadian alignment. The available evidence on relationships between 2 internal phase markers (e.g., dim light melatonin onset [DLMO] and peak cortisol concentration) suggests these signals may be temporally misaligned in major depressive disorder with greater misalignment associated with more severe depressive symptoms. This study aimed to examine multiple circadian phase markers to determine whether any youth with emerging mood disorders present with clear evidence of internal circadian misalignment, and whether the degree of circadian misalignment is correlated with more severe mood symptoms. Cross-sectional data from 69 youth presenting for mental health care (20.6 ± 3.8 years; 39% male) and 19 healthy controls (24.0 ± 3.6 years; 53% male) included actigraphy monitoring; overnight in-lab measurement of 3 phase markers: DLMO, salivary cortisol peak (CORT), and core body temperature nadir (TEMP); and depressive symptoms (Hamilton Depression Rating Scale). Abnormal phase angles between 2 phase markers were defined as ±2 standard deviations beyond the control mean. In those with emerging mood disorders, earlier TEMP relative to other phase markers (DLMO, CORT, sleep midpoint) was associated with higher depressive symptoms. Sixteen individuals (23%) with emerging mood disorders had abnormal phase angles between at least 1 pair of phase markers, consistent with internal misalignment of the circadian system. The internal misalignment subgroup had later DLMO on average, however presented with a diverse range of individual phase angle abnormalities. Diverse disruptions of circadian alignment occur in youth with mental ill-health. The relative timing of core body temperature and melatonin rhythms may be key circadian features linked to depressive symptoms. Longitudinal research is needed to establish whether correction of circadian misalignment is relevant to treatment of mood syndromes in youth with evidence of disrupted circadian systems.</p>","PeriodicalId":15056,"journal":{"name":"Journal of Biological Rhythms","volume":" ","pages":"424-440"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Hard Day's Night: The Circadian Beatles.","authors":"Diego A Golombek","doi":"10.1177/07487304251365297","DOIUrl":"10.1177/07487304251365297","url":null,"abstract":"","PeriodicalId":15056,"journal":{"name":"Journal of Biological Rhythms","volume":" ","pages":"409-410"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asymmetric Velocity Response Curves to Light and the Intrinsic Period of the Human Circadian Pacemaker.","authors":"Anne C Skeldon, Imran M Usmani, Derk-Jan Dijk","doi":"10.1177/07487304251355454","DOIUrl":"10.1177/07487304251355454","url":null,"abstract":"","PeriodicalId":15056,"journal":{"name":"Journal of Biological Rhythms","volume":" ","pages":"421-423"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circadian Rhythms in Health and Disease (CRHD 2024).","authors":"Sandipan Ray, Srishti Banerjee","doi":"10.1177/07487304251349350","DOIUrl":"10.1177/07487304251349350","url":null,"abstract":"","PeriodicalId":15056,"journal":{"name":"Journal of Biological Rhythms","volume":" ","pages":"411-413"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Takao Kondo.","authors":"Hideo Iwasaki, Ayano Ogihara, Ikuno Ohashi, Ken-Ichi Honma, Takashi Yoshimura, Susan S Golden, Carl Hirschie Johnson","doi":"10.1177/07487304251352036","DOIUrl":"10.1177/07487304251352036","url":null,"abstract":"","PeriodicalId":15056,"journal":{"name":"Journal of Biological Rhythms","volume":" ","pages":"403-408"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}