Journal of Cell Science and Therapy最新文献_第6页

Glucan from Food Supplement to a Licensed Drug 从食品补充剂到许可药物葡聚糖

Journal of Cell Science and Therapy Pub Date : 2015-10-09 DOI: 10.4172/2157-7013.1000232

V. Vaclav

引用次数: 1

The Antidiabetic Effect of Mesenchymal Stem Cells vs. Nigella sativa Oil onStreptozotocin Induced Type 1 Diabetic Rats 间充质干细胞对链脲佐菌素诱导的1型糖尿病大鼠的降糖作用

Journal of Cell Science and Therapy Pub Date : 2015-10-05 DOI: 10.4172/2157-7013.1000226

S. Mohamed, Eman A Ali, S. Hosny

{"title":"The Antidiabetic Effect of Mesenchymal Stem Cells vs. Nigella sativa Oil onStreptozotocin Induced Type 1 Diabetic Rats","authors":"S. Mohamed, Eman A Ali, S. Hosny","doi":"10.4172/2157-7013.1000226","DOIUrl":"https://doi.org/10.4172/2157-7013.1000226","url":null,"abstract":"Introduction: Diabetes is a chronic metabolic disorder that has no definitive cure till present. Currently; regenerative medicine using Mesenchymal Stem Cells (MSCs) offers promising treatment. Meanwhile, Nigella Sativa Oil (NSO) shows effectiveness for medication of various illnesses. \u0000Objective: This study compares the antidiabetic effect of MSCs vs. NSO on streptozotocin induced type 1 diabetes (T1D) in rat model. \u0000Methods: Human cord blood samples were collected. Isolated mononuclear cells were cultured and incubated to isolate MSCs. For experimental animals; after induction of T1D using multiple low dose streptozotocin (MLD-STZ), diabetic rats were divided into 6 groups (n =10); diabetic group 1 and 2 (sacrificed 15 and 30 days post-induction, consequently), NSO treated groups 1 and 2 (received daily NSO I.P. injection, sacrificed 15 and 30 days postinduction, consequently), MSCs treated groups 1 and 2 (72 hours post-induction, animals were treated with twice intravenous injection of 1×106 cells/rat, 24 hours apart, without immunosuppression, sacrificed 15 and 30 days of induction, consequently). \u0000Results: Low blood glucose levels were detected in both MSCs and NSO groups, which reached near normal levels within 15 days. This was accompanied by improvement of the histopathological changes and increase in the immunreactive insulin secreting cells, obviously detected after 30 days. \u0000Conclusion: MSCs and NSO exerts comparable antidiabetic effects on Streptozotocin induced T1D.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127665025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Antagonists of the Mineralocorticoid Receptor or A New Pharmacological Class 矿物皮质激素受体拮抗剂或一类新的药理类

Journal of Cell Science and Therapy Pub Date : 2015-09-30 DOI: 10.4172/2157-7013.1000222

M. M. Hermidorff, L. D. Assis, M. C. Isoldi

引用次数: 4

Can GDF-15 be Used to Assess ventricular recovery following Left Ventricular Assist Device Therapy? GDF-15可以用于评估左心室辅助装置治疗后的心室恢复吗?

Journal of Cell Science and Therapy Pub Date : 2015-09-30 DOI: 10.4172/2157-7013.S8-007

ini Nair, E. Góngora

引用次数: 1

Breast Microcalcifications: A Focus 乳房微钙化:一个焦点

Journal of Cell Science and Therapy Pub Date : 2015-09-30 DOI: 10.4172/2157-7013.S8-E101

M. Scimeca, Chiara Antonacci, E. Bonanno

引用次数: 12

Bystander Effects Induced by the Monolayer and Three Dimensional Cultures Exposed to Ionizing Radiation 电离辐射下单层和三维培养物诱导的旁观者效应

Journal of Cell Science and Therapy Pub Date : 2015-09-29 DOI: 10.4172/2157-7013.S8-005

R. Wu, Yaxiong Chen, Yarong Du, G. Du, Burong Hu, Lijun Wu

引用次数: 1

Blastic Plasmacytoid Dendritic Cell Neoplasm: A Review of Diagnosis, Pathology, and Therapy 母浆细胞样树突状细胞肿瘤:诊断、病理和治疗的综述

Journal of Cell Science and Therapy Pub Date : 2015-09-28 DOI: 10.4172/2157-7013.S8-008

R. Shapiro, N. Sangle, Michael Keeney, I. C. Yee, C. Hsia, Selay Lam

{"title":"Blastic Plasmacytoid Dendritic Cell Neoplasm: A Review of Diagnosis, Pathology, and Therapy","authors":"R. Shapiro, N. Sangle, Michael Keeney, I. C. Yee, C. Hsia, Selay Lam","doi":"10.4172/2157-7013.S8-008","DOIUrl":"https://doi.org/10.4172/2157-7013.S8-008","url":null,"abstract":"Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare malignancy of dendritic cell precursors classified as a subset of acute myeloid leukemia according to WHO. It commonly presents with dermal infiltration of plasmatoid dendritic cells that have the morphologic appearance of medium-sized blasts with irregular nuclei, faint chromatin, at least one nucleolus per cell and scant cytoplasm, expressing a CD4+CD56+CD123+lin- immunophenotype. Patients typically have discoloured cutaneous lesions that grow in size, and the diagnosis of BPDCN is confirmed on skin biopsy showing the characteristic cells. Bone marrow involvement is a common feature of this neoplasm and is found in most patients at diagnosis. There is no consensus on the most appropriate treatment for BPDCN, with the neoplasm showing initial good response to high intensity chemotherapy but inevitable relapse into a more chemotherapy-resistant disease. Hematopoietic stem cell transplant in patients who achieve their first complete remission with chemotherapy is a promising therapeutic modality requiring a prospective clinical trial to evaluate its efficacy.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130822822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Therapeutic and Protective Effect of Wheat Germ Oil on L-arginine Induced Acute Pancreatitis in Adult Albino Rats 小麦胚芽油对l -精氨酸诱导的成年白化大鼠急性胰腺炎的治疗和保护作用

Journal of Cell Science and Therapy Pub Date : 2015-09-24 DOI: 10.4172/2157-7013.S8-004

S. Gawad

{"title":"Therapeutic and Protective Effect of Wheat Germ Oil on L-arginine Induced Acute Pancreatitis in Adult Albino Rats","authors":"S. Gawad","doi":"10.4172/2157-7013.S8-004","DOIUrl":"https://doi.org/10.4172/2157-7013.S8-004","url":null,"abstract":"Background: Acute pancreatitis (AP) is a disease that still has high morbidity and mortality rates. Oxygen free radicals and cytokines have been mentioned to play a role in the development of L-arginine induced AP. There is no specific therapy for AP. Herbals, as botanical medical treatments, have generated a deal of public controversy in recent years. Wheat germ oil (WGO) is one of these treatments that possess anti-inflammatory properties. \u0000Objective: To investigate the possible protective and therapeutic effect of wheat germ oil on L-arginine induced AP in adult albino rats. \u0000Methods: Forty adult male albino rats were divided equally into five groups: Group I (control group). Group II (WGO group): received WGO by oral gavage once daily for 3 successive days. Group III (AP group): received double IP injection of L-arginine with 1 h interval. Group IV (protection group): received WGO as group II, followed by Larginine injection as group III. Group V (therapeutic group): received L-arginine followed by WGO. Blood samples were taken for biochemical assessment of serum lipase to confirm induction of AP. At the end of the experiment, animals were sacrificed and specimens from head of the pancreas were taken and prepared for light, electron microscopic examinations and ELISA detection of interleukin-1β. \u0000Results: AP and protected groups showed extensive acinar cell damage in the form of loss of basal basophilia, necrotic changes, interstitial edema, severe inflammatory infiltration and hemorrhage. These groups showed statistical significant increase in the mean of pancreatic damage compared to control group (p<0.05). Ultrastructurally, the acinar cells showed dense heterochromatic nuclei with many degraded electron-lucent zymogen granules and swollen, disarranged rER. However, the acini showed significant improvement in the therapeutic group. \u0000Conclusion: WGO has a therapeutic not a protective effect on L-arginine-induced AP which might be through its anti-inflammatory effects.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128810645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

An Overview of the Parameters for Recombinant Protein Expression in Escherichia coli 重组蛋白在大肠杆菌中的表达参数综述

Journal of Cell Science and Therapy Pub Date : 2015-08-29 DOI: 10.4172/2157-7013.1000221

B. C. Joseph, Suthakaran Pichaimuthu, S. Srimeenakshi, M. Murthy, K. Selvakumar, M. Ganesan, Sadan, A. Manjunath

引用次数: 60

Stability of Selected Hematological Parameters in Stored Blood Samples 储存血液样本中选定血液学参数的稳定性

Journal of Cell Science and Therapy Pub Date : 2015-08-16 DOI: 10.4172/2157-7013.1000220

A. Tendulkar, Puneet Jain, S. Gujral, Manisha Tambe, R. Kenjale, B. Ganesh

{"title":"Stability of Selected Hematological Parameters in Stored Blood Samples","authors":"A. Tendulkar, Puneet Jain, S. Gujral, Manisha Tambe, R. Kenjale, B. Ganesh","doi":"10.4172/2157-7013.1000220","DOIUrl":"https://doi.org/10.4172/2157-7013.1000220","url":null,"abstract":"1.1 Background: Apheresis platelets are a mainstay therapy in our tertiary cancer centre wherein 3000 donors are tested annually. Platelet drives to register willing donors are conducted. The complete blood counts to determine the eligibility for platelet donation is done on the first donor visit. However, it can be challenging to test these samples on that day due to reasons like manpower shortages, weekends, and a single cell counter in blood banks. National guidelines for blood banks in India do not elaborate on the ideal storage time for such blood samples. Hence we embarked on this study to determine the sample stability. Aim: The aim was to study the stability of blood samples given at first visit for registration as platelet donors. Specific hematologic parameters were studied at time intervals of 4, 48 and 72 hours. Materials and Methods: Eligible donor’s venous blood sample was collected in K2-EDTA (Ethylenediaminetetraacetic acid) vacutainers for cell counts. This was tested on an automated cell counter (Humacount, Human, Germany) within 4 hours at room temperature. Then, the samples were stored at 4oC and retested at 48 and 72 hours. Results: 969 blood samples were tested for hemoglobin, WBC (White blood cell) and Platelet count. There was no statistical difference in mean values of hemoglobin and WBC counts at three time intervals. Even though difference in mean platelet count was statistically significant (p<0.001), it did not impact on donor acceptance criteria. Conclusions: Specific hematologic parameters (Hb, WBC, platelet) were found to be stable at 4oC for 72 hours. Hemoglobin (Hb) was the best preserved parameter followed by WBC and platelet count. J o u r na l o f C ell Science & Tera p y ISSN: 2157-7013 Journal of Cell Science & Therapy Citation: Tendulkar A, Jain P, Gujral S, Tambe M, Kenjale R, et al. (2015) Stability of Selected Hematological Parameters in Stored Blood Samples. J Cell Sci Ther 6: 220. doi:10.4172/2157-7013.1000220","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123698605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12