Stability of Selected Hematological Parameters in Stored Blood Samples

Journal of Cell Science and Therapy Pub Date : 2015-08-16 DOI:10.4172/2157-7013.1000220

A. Tendulkar, Puneet Jain, S. Gujral, Manisha Tambe, R. Kenjale, B. Ganesh

{"title":"Stability of Selected Hematological Parameters in Stored Blood Samples","authors":"A. Tendulkar, Puneet Jain, S. Gujral, Manisha Tambe, R. Kenjale, B. Ganesh","doi":"10.4172/2157-7013.1000220","DOIUrl":null,"url":null,"abstract":"1.1 Background: Apheresis platelets are a mainstay therapy in our tertiary cancer centre wherein 3000 donors are tested annually. Platelet drives to register willing donors are conducted. The complete blood counts to determine the eligibility for platelet donation is done on the first donor visit. However, it can be challenging to test these samples on that day due to reasons like manpower shortages, weekends, and a single cell counter in blood banks. National guidelines for blood banks in India do not elaborate on the ideal storage time for such blood samples. Hence we embarked on this study to determine the sample stability. Aim: The aim was to study the stability of blood samples given at first visit for registration as platelet donors. Specific hematologic parameters were studied at time intervals of 4, 48 and 72 hours. Materials and Methods: Eligible donor’s venous blood sample was collected in K2-EDTA (Ethylenediaminetetraacetic acid) vacutainers for cell counts. This was tested on an automated cell counter (Humacount, Human, Germany) within 4 hours at room temperature. Then, the samples were stored at 4oC and retested at 48 and 72 hours. Results: 969 blood samples were tested for hemoglobin, WBC (White blood cell) and Platelet count. There was no statistical difference in mean values of hemoglobin and WBC counts at three time intervals. Even though difference in mean platelet count was statistically significant (p<0.001), it did not impact on donor acceptance criteria. Conclusions: Specific hematologic parameters (Hb, WBC, platelet) were found to be stable at 4oC for 72 hours. Hemoglobin (Hb) was the best preserved parameter followed by WBC and platelet count. J o u r na l o f C ell Science & Tera p y ISSN: 2157-7013 Journal of Cell Science & Therapy Citation: Tendulkar A, Jain P, Gujral S, Tambe M, Kenjale R, et al. (2015) Stability of Selected Hematological Parameters in Stored Blood Samples. J Cell Sci Ther 6: 220. doi:10.4172/2157-7013.1000220","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"2 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2015-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"12","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cell Science and Therapy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2157-7013.1000220","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 12

Abstract

1.1 Background: Apheresis platelets are a mainstay therapy in our tertiary cancer centre wherein 3000 donors are tested annually. Platelet drives to register willing donors are conducted. The complete blood counts to determine the eligibility for platelet donation is done on the first donor visit. However, it can be challenging to test these samples on that day due to reasons like manpower shortages, weekends, and a single cell counter in blood banks. National guidelines for blood banks in India do not elaborate on the ideal storage time for such blood samples. Hence we embarked on this study to determine the sample stability. Aim: The aim was to study the stability of blood samples given at first visit for registration as platelet donors. Specific hematologic parameters were studied at time intervals of 4, 48 and 72 hours. Materials and Methods: Eligible donor’s venous blood sample was collected in K2-EDTA (Ethylenediaminetetraacetic acid) vacutainers for cell counts. This was tested on an automated cell counter (Humacount, Human, Germany) within 4 hours at room temperature. Then, the samples were stored at 4oC and retested at 48 and 72 hours. Results: 969 blood samples were tested for hemoglobin, WBC (White blood cell) and Platelet count. There was no statistical difference in mean values of hemoglobin and WBC counts at three time intervals. Even though difference in mean platelet count was statistically significant (p<0.001), it did not impact on donor acceptance criteria. Conclusions: Specific hematologic parameters (Hb, WBC, platelet) were found to be stable at 4oC for 72 hours. Hemoglobin (Hb) was the best preserved parameter followed by WBC and platelet count. J o u r na l o f C ell Science & Tera p y ISSN: 2157-7013 Journal of Cell Science & Therapy Citation: Tendulkar A, Jain P, Gujral S, Tambe M, Kenjale R, et al. (2015) Stability of Selected Hematological Parameters in Stored Blood Samples. J Cell Sci Ther 6: 220. doi:10.4172/2157-7013.1000220

查看原文本刊更多论文

储存血液样本中选定血液学参数的稳定性

1.1背景:单采血小板是我们三级癌症中心的主要治疗方法，每年对3000名献血者进行检测。开展血小板捐献活动，登记自愿献血者。确定血小板捐献资格的全血细胞计数在第一次献血者就诊时完成。然而，由于人力短缺、周末和血库的单细胞计数器等原因，在当天检测这些样本可能具有挑战性。印度国家血库指南没有详细说明这类血液样本的理想储存时间。因此，我们开始了这项研究，以确定样品的稳定性。目的:研究首次就诊登记血小板献血者血样的稳定性。在4、48和72小时的时间间隔内研究特定的血液学参数。材料与方法:取符合条件的献血者静脉血，用K2-EDTA(乙二胺四乙酸)真空容器进行细胞计数。在室温下4小时内在自动细胞计数器(humaccount, Human, Germany)上进行测试。然后，样品在4℃保存，并在48和72小时重新测试。结果:对969份血样进行了血红蛋白、白细胞和血小板计数检测。血红蛋白和白细胞计数在三个时间间隔内的平均值无统计学差异。尽管平均血小板计数差异有统计学意义(p<0.001)，但对供体接受标准没有影响。结论:特定血液学参数(Hb, WBC，血小板)在4oC下保持72小时稳定。血红蛋白(Hb)是保存最好的参数，其次是白细胞和血小板计数。[J]刘建军，刘建军，刘建军，等。(2015)血液样品中血液学参数的稳定性研究[J] .中国生物医学工程学报，vol . 32(1): 1 -7。[J]中国生物医学工程学报(英文版)。doi: 10.4172 / 2157 - 7013.1000220

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Cell Science and Therapy

自引率

0.00%

发文量