Journal of applied glycoscience最新文献

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Characterization of the Interactions between Cereal Flour and "Nata Puree" in Batter. 面糊中谷物粉与“纳塔泥”相互作用的表征。
IF 1.1
Journal of applied glycoscience Pub Date : 2022-01-01 DOI: 10.5458/jag.jag.JAG-2022_0007
Ken Tokuyasu, Junko Matsuki, Kenji Yamagishi, Masakazu Ike
{"title":"Characterization of the Interactions between Cereal Flour and \"Nata Puree\" in Batter.","authors":"Ken Tokuyasu, Junko Matsuki, Kenji Yamagishi, Masakazu Ike","doi":"10.5458/jag.jag.JAG-2022_0007","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2022_0007","url":null,"abstract":"This study aimed to characterize the interactions between cereal flour (rice, wheat, and barley) and “nata puree” (NP), a disintegrated bacterial cellulose (BC) in the presence of a water-soluble polysaccharide, with powder-dispersion activity. Pasting properties of cereal flour with additives were analyzed using a Rapid Visco Analyzer, and disintegrated BC in water (BCW), three water-soluble polysaccharides: (1,3)(1,4)-β-glucan, tamarind seed gum, and birchwood xylan, and the corresponding NPs were used as additives. For rice flour, additional BCW or NPs increased the initial and the peak viscosity. The addition of water-soluble polysaccharides produced the opposite trend: viscosity increased from the peak time to the end of measurements. For wheat flour, the addition of BCW or NP delayed the peak time and increased peak viscosity; the increase was maintained till the end of measurements. For barley flour, the additional BCW or NP caused a higher gelatinization rate and increased viscosity at the starch-retrogradation stage. Next, static gelatinization of a rice flour suspension in NP was successfully accomplished before placing it in a vessel; NP concentration in the gel significantly affected the firmness. Thus, the dynamic and unique interactions between various cereal flours and cell-wall polysaccharides in NPs can increase the flours' potential; static gelatinization of cereal flour with NP could expand flours' application range in both current and next-generation cooking.","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"69 4","pages":"73-81"},"PeriodicalIF":1.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/37/f0/69_jag.JAG-2022_0007.PMC9720633.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10766413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cabbage Core Powder as a New Food Material for Paste Preparation with "Nata Puree". 白菜芯粉作为一种新型食品原料制备“纳塔果泥”膏体。
IF 1.1
Journal of applied glycoscience Pub Date : 2022-01-01 DOI: 10.5458/jag.jag.JAG-2022_0003
Ken Tokuyasu, Kenji Yamagishi, Yasumasa Ando, Nobuya Shirai
{"title":"Cabbage Core Powder as a New Food Material for Paste Preparation with \"Nata Puree\".","authors":"Ken Tokuyasu,&nbsp;Kenji Yamagishi,&nbsp;Yasumasa Ando,&nbsp;Nobuya Shirai","doi":"10.5458/jag.jag.JAG-2022_0003","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2022_0003","url":null,"abstract":"<p><p>Cabbage core (CC) is regarded as a waste part of the vegetable, despite being edible and containing various nutritional and functional compounds. We investigated the properties of CC powder with particle sizes < 1 mm as a new food material. CC powder was more resistant to structural deformation than leaf-derived powder, particularly CC powder with particles ≥ 0.3 mm in size. To examine the application of CC powder in 3D printed foods, we investigated the effects of \"nata puree,\" a disintegrated nata de coco made with tamarind seed gum (NPTG), on paste made with CC powder. NPTG promoted stable binding of paste made using CC powder, which was successfully extruded using a syringe to form a bar with a granular structure. Thus, CC powder possesses unique textural/structural properties for its application in next-generation foods.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"69 4","pages":"91-95"},"PeriodicalIF":1.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/db/65/69_jag.JAG-2022_0003.PMC9720631.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10766411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microorganisms Capable of Producing Polysaccharides from D-Xylose. 能够从d -木糖中产生多糖的微生物。
IF 1.1
Journal of applied glycoscience Pub Date : 2022-01-01 DOI: 10.5458/jag.jag.JAG-2022_0008
Sosyu Tsutsui, Tomohiro Hatano, Ryo Funada, Satoshi Kaneko
{"title":"Microorganisms Capable of Producing Polysaccharides from D-Xylose.","authors":"Sosyu Tsutsui,&nbsp;Tomohiro Hatano,&nbsp;Ryo Funada,&nbsp;Satoshi Kaneko","doi":"10.5458/jag.jag.JAG-2022_0008","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2022_0008","url":null,"abstract":"<p><p>In recent years, the importance of biomass utilization has increased, but it has not been effectively exploited. In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass. Therefore, in order to promote the utilization of hemicellulose, we screened for microorganisms capable of producing polysaccharides from D-xylose. The following four strains were selected from samples collected from various regions of Okinawa Prefecture: <i>Kosakonia</i> sp. (SO_001), <i>Papiliotrema terrestris</i> (SO_005), <i>Pseudarthrobacter</i> sp. (SO_006), and <i>Williamsia</i> sp. (SO_009). Observation with a scanning electron microscope (SEM) confirmed that each bacterium produced polysaccharides with different shapes. In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct. The selected microorganisms include closely related species known to promote plant growth and known to suppress postharvest pathogens. Since these microorganisms may be used not only in known fields but also in new fields, the results of this research are expected to greatly expand the uses of hemicellulose.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"69 4","pages":"83-89"},"PeriodicalIF":1.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bd/24/69_jag.JAG-2022_0008.PMC9720630.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10749731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Long-term D-Allose Administration Favorably Alters the Intestinal Environment in Aged Male Mice. 长期服用D-Allose有利于改变老年雄性小鼠肠道环境。
IF 1.1
Journal of applied glycoscience Pub Date : 2022-01-01 DOI: 10.5458/jag.jag.JAG-2022_0005
Tomoya Shintani, Shuichi Yanai, Akane Kanasaki, Misuzu Tanaka, Tetsuo Iida, Genki Ozawa, Tadao Kunihiro, Shogo Endo
{"title":"Long-term D-Allose Administration Favorably Alters the Intestinal Environment in Aged Male Mice.","authors":"Tomoya Shintani,&nbsp;Shuichi Yanai,&nbsp;Akane Kanasaki,&nbsp;Misuzu Tanaka,&nbsp;Tetsuo Iida,&nbsp;Genki Ozawa,&nbsp;Tadao Kunihiro,&nbsp;Shogo Endo","doi":"10.5458/jag.jag.JAG-2022_0005","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2022_0005","url":null,"abstract":"<p><p>D-Allose, a C3 epimer of D-glucose, has potential to improve human health as a functional food. However, its effect on the intestinal environment remains unknown. Aged humans progressively express changes in the gut, some of which deleteriously affect gastrointestinal health. In this study, we profiled the intestinal microbiome in aged mice and analyzed organic acids produced by bacteria in cecum contents after long-term ingestion of D-allose. D-Allose did not significantly change organic acid concentration. However, long-term ingestion did significantly increase the relative abundance of Actinobacteria and reduce the relative abundance of Proteobacteria. These results suggest that oral D-allose improves the proportion of favorable intestinal flora in aged mice. D-Allose significantly decreased the relative abundance of Lachnospiraceae bacteria, but increased the relative abundance of <i>Bacteroides acidifaciens</i> and <i>Akkermansia muciniphila</i>. Thus, D-allose might serve as a nutraceutical capable of improving the balance of gut microbiome during aging.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"69 4","pages":"97-102"},"PeriodicalIF":1.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d7/43/69_jag.JAG-2022_0005.PMC9720632.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10749733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
An Organogermanium Compound Enhances the Initial Reaction Rate of Alkaline Isomerization of an Aldose into a Ketose through Enediol Complex Formation 一种有机锗化合物通过形成烯二醇络合物提高醛糖碱性异构化成酮糖的初始反应速率
IF 1.1
Journal of applied glycoscience Pub Date : 2021-12-25 DOI: 10.5458/jag.jag.JAG-2021_0011
Katsuyuki Sato, Takae Nagasawa, T. Kasumi
{"title":"An Organogermanium Compound Enhances the Initial Reaction Rate of Alkaline Isomerization of an Aldose into a Ketose through Enediol Complex Formation","authors":"Katsuyuki Sato, Takae Nagasawa, T. Kasumi","doi":"10.5458/jag.jag.JAG-2021_0011","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2021_0011","url":null,"abstract":"We previously demonstrated that the organogermanium compound 3-(trihydroxygermyl)propanoic acid (THGP) enhances the enzymatic and alkaline isomerization of an aldose to a ketose through cis-diol complex formation by multiple mechanisms. Its higher affinity for the ketose than the aldose protects the ketose complex from alkaline decomposition. Furthermore, it has been reported that the aldose-ketose alkaline isomerization pathway includes 1,2-enediol. Therefore, we speculated that the complex-forming ability of THGP could also be applied to enediol, a transient intermediate of alkaline isomerization. To test this prediction, we analyzed the initial rates of glucose or lactose isomerization in a region where there was no substantial difference in pH with and without THGP addition. The results showed that THGP enhanced the rate of fructose or lactulose formation per unit time by approximately 2-fold compared to the control. This finding indicated that THGP could form a complex with the transition state of aldose-ketose alkaline isomerization.","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"11 1","pages":"9 - 13"},"PeriodicalIF":1.1,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74389299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Trehalose Content and Water Activity on the Fracture Properties of Deep-fried Wheat Flour Particles and Freeze-dried Porous Waxy Corn Starch Solids. 海藻糖含量和水分活度对油炸小麦粉颗粒和冻干多孔蜡质玉米淀粉固体破碎性能的影响。
IF 1.1
Journal of applied glycoscience Pub Date : 2021-11-20 eCollection Date: 2021-01-01 DOI: 10.5458/jag.jag.JAG-2021_0007
Trinh Ngoc Dang Le, Akane Matsumoto, Kiyoshi Kawai
{"title":"Effects of Trehalose Content and Water Activity on the Fracture Properties of Deep-fried Wheat Flour Particles and Freeze-dried Porous Waxy Corn Starch Solids.","authors":"Trinh Ngoc Dang Le,&nbsp;Akane Matsumoto,&nbsp;Kiyoshi Kawai","doi":"10.5458/jag.jag.JAG-2021_0007","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2021_0007","url":null,"abstract":"<p><p>Wheat flour-based batter containing 0 to 20 % trehalose was deep-fried, dried and held in various water activity (<i>a</i> <sub>w</sub>) conditions. The effects of trehalose content and <i>a</i> <sub>w</sub> on oil content, water sorption, isothermal mechanical relaxation, and fracture properties were investigated. For comparison, the fracture properties of freeze-dried porous waxy corn starch solids were also investigated. The 10 % trehalose sample had the lowest oil content, water content, and <i>a</i> <sub>w</sub>. A force-reduction value (∆<i>F</i>) of the samples was evaluated as a typical mechanical relaxation parameter. ∆<i>F</i> gradually increased with increasing <i>a</i> <sub>w</sub> and sharply increased above a specific <i>a</i> <sub>w</sub> presumed to be associated with the glass to rubber transition. Compared to ∆<i>F</i> values among the glassy samples, 10 and 20 % trehalose samples had higher ∆<i>F</i> values (were more rigid) than 0 and 5 % trehalose samples. From the fracture measurements of the glassy samples, the first fracture force increased linearly and the number of fracture peaks decreased linearly with increasing <i>a</i> <sub>w</sub>. At each <i>a</i> <sub>w</sub>, 10 % trehalose had the lowest first fracture force and the highest the number of fracture peaks. Freeze-dried porous waxy corn starch solids showed similar fracture properties to deep-fried samples. These findings suggest that around 10 % trehalose content is optimal for producing deep-fried foods with a brittle texture.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"68 4","pages":"69-76"},"PeriodicalIF":1.1,"publicationDate":"2021-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c1/08/JAG-68-69.PMC8611405.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39772849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
"Nata Puree," a Novel Food Material for Upgrading Vegetable Powders, Made by Bacterial Cellulose Gel Disintegration in the Presence of (1,3)(1,4)-β-Glucan. 在(1,3)(1,4)-β-葡聚糖存在下,由细菌纤维素凝胶分解制成的“纳塔果泥”,一种用于升级蔬菜粉的新型食品材料。
IF 1.1
Journal of applied glycoscience Pub Date : 2021-11-20 eCollection Date: 2021-01-01 DOI: 10.5458/jag.jag.JAG-2021_0009
Ken Tokuyasu, Kenji Yamagishi, Junko Matsuki, Daisuke Nei, Tomoko Sasaki, Masakazu Ike
{"title":"\"Nata Puree,\" a Novel Food Material for Upgrading Vegetable Powders, Made by Bacterial Cellulose Gel Disintegration in the Presence of (1,3)(1,4)-β-Glucan.","authors":"Ken Tokuyasu,&nbsp;Kenji Yamagishi,&nbsp;Junko Matsuki,&nbsp;Daisuke Nei,&nbsp;Tomoko Sasaki,&nbsp;Masakazu Ike","doi":"10.5458/jag.jag.JAG-2021_0009","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2021_0009","url":null,"abstract":"<p><p>Pulverization is a potentially powerful solution for the resource management of surplus- and non-standard agricultural products, maintaining their nutritional values for long and ensuring their homogeneity, whereas their original textures could disappear to narrow the application ranges. Therefore, new technologies should be developed for reconstructing the powders to provide them with new physical characteristics. Herein, we developed a novel food material, nata puree (NP), by nata de coco (bacterial cellulose gel) disintegration with a water-soluble polysaccharide using a household blender. The process worked well with (1,3)(1,4)-β-glucan (BGL) as the polysaccharide, which could be substituted with barley extract. Lichenase treatment of the NP dramatically modified its physical properties, suggesting the importance of the BGL polymeric forms. NP exhibited distinct potato powder and starch binding activities, which would be attributed to its interactions with the cell wall components and a physical capture of powders by the NP network, respectively. NP supplementation into the potato paste improved its firmness and enabled its printable range shift for 3D food printing to a lower powder-concentration. NP also promoted the dispersion of powders in its suspension, and designed gelation could also be successfully performed by the laser irradiation of an NP suspension containing dispersed curdlan and turmeric powders. Therefore, NP could be applied as a powder modifier to a wide range of products in both conventional cooking, food manufacturing, and next generation processes such as 3D food printing.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"68 4","pages":"77-87"},"PeriodicalIF":1.1,"publicationDate":"2021-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/87/a1/JAG-68-077.PMC8611406.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39772850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Preparation and Analysis of α-1,6 Glucan as a Slowly Digestible Carbohydrate. α-1,6葡聚糖慢消化碳水化合物的制备与分析。
IF 1.1
Journal of applied glycoscience Pub Date : 2021-08-22 eCollection Date: 2021-01-01 DOI: 10.5458/jag.jag.JAG-2021_0001
Kenta Aizawa, Hiroki Takagi, Eri Kokubo, Masayasu Takada
{"title":"Preparation and Analysis of α-1,6 Glucan as a Slowly Digestible Carbohydrate.","authors":"Kenta Aizawa,&nbsp;Hiroki Takagi,&nbsp;Eri Kokubo,&nbsp;Masayasu Takada","doi":"10.5458/jag.jag.JAG-2021_0001","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2021_0001","url":null,"abstract":"<p><p>Carbohydrate materials that produce lower postprandial blood glucose increase are required for diabetic patients. To develop slowly digestible carbohydrates, the effect of degree of polymerization (DP) of α-1,6 glucan on its digestibility was investigated <i>in vitro</i> and <i>in vivo</i>. We prepared four fractions of α-1,6 glucan composed primarily of DP 3-9, DP 10-30, DP 31-150, and DP 151+ by fractionating a dextran hydrolysate. An <i>in vitro</i> experiment using digestive enzymes showed that the glucose productions of DP 3-9, DP 10-30, DP 31-150, and DP 151+ were 70.3, 53.4, 28.2, and 19.2 % in 2 h, and 92.1, 83.9, 39.6, and 33.3 % in 24 h relative to dextrin, respectively. An <i>in vivo</i> glycemic response showed that the incremental area under the curve (iAUC) of blood glucose levels of α-1,6 glucan with DP 3-9, DP 10-30, DP 31-150, and DP 151+ were 99.5, 84.3, 65.4, and 40.1 % relative to dextrin, respectively. These results indicated that α-1,6 glucan with higher DP had stronger resistance to digestion and produced a smaller blood glucose response. DP 10-30 showed significantly lower maximum blood glucose levels than dextrin; however, no significant difference was observed in iAUC, indicating that DP 10-30 was slowly digestible. In addition, α-1,6 glucan was also produced using an enzymatic reaction with dextrin dextranase (DDase). This produced similar results to DP 10-30. The DDase product can be synthesized from dextrin at low cost. This glucan is expected to be useful as a slowly digestible carbohydrate source.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"68 3","pages":"53-61"},"PeriodicalIF":1.1,"publicationDate":"2021-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/a2/JAG-68-53.PMC8575654.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39875717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of the Enzymatic Saccharification Efficiency of an Energy Crop, Erianthus arundinaceus, Pretreated with Ca(OH)2 Using both Countercurrent Washing System and pH Adjustment by Nonpressurized CO2. 用Ca(OH)2逆流洗涤和无加压CO2调节pH对能源作物鸢尾花酶解糖化效率的评价
IF 1.1
Journal of applied glycoscience Pub Date : 2021-07-22 eCollection Date: 2021-01-01 DOI: 10.5458/jag.jag.JAG-2021_0005
Kenji Yamagishi, Masakazu Ike, Mitsuru Gau, Ken Tokuyasu
{"title":"Evaluation of the Enzymatic Saccharification Efficiency of an Energy Crop, <i>Erianthus arundinaceus</i>, Pretreated with Ca(OH)<sub>2</sub> Using both Countercurrent Washing System and pH Adjustment by Nonpressurized CO<sub>2</sub>.","authors":"Kenji Yamagishi,&nbsp;Masakazu Ike,&nbsp;Mitsuru Gau,&nbsp;Ken Tokuyasu","doi":"10.5458/jag.jag.JAG-2021_0005","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2021_0005","url":null,"abstract":"<p><p><i>Erianthus arundinaceus</i> (ER) is greatly appreciated among domestic energy crops in Japan for the production of fermentable sugars from lignocellulosic polysaccharides. In this study, we developed an efficient Ca(OH)<sub>2</sub>-based pretreatment of both stems and leaves of ER at ambient temperature with the addition of a washing step for enzymatic saccharification. The recoveries of glucans and xylans in the pretreated ER after four countercurrent washing cycles were 91 and 76 %, respectively, the former being considerably higher than that of rice straw (RS) (72 %). Their saccharification ratios in the washed sample under the pressure of 1 atm CO<sub>2</sub> were 80 and 92.5 %, respectively. The application of this simple sugar production process from ER would further support the domestic bioprocess development. ER is also foreseen to provide the additional feedstock favorable for harvesting from winter to spring in Japan, preventing a risk for feedstock shortage generated by single harvesting such as RS.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"68 3","pages":"63-67"},"PeriodicalIF":1.1,"publicationDate":"2021-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/a7/JAG-68-63.PMC8575653.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39875718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of a GH36 α-D-Galactosidase Associated with Assimilation of Gum Arabic in Bifidobacterium longum subsp. longum JCM7052. 与长双歧杆菌阿拉伯胶同化相关的GH36 α- d -半乳糖苷酶的鉴定longum JCM7052。
IF 1.1
Journal of applied glycoscience Pub Date : 2021-06-11 eCollection Date: 2021-01-01 DOI: 10.5458/jag.jag.JAG-2021_0004
Yuki Sasaki, Yumi Uchimura, Kanefumi Kitahara, Kiyotaka Fujita
{"title":"Characterization of a GH36 α-D-Galactosidase Associated with Assimilation of Gum Arabic in <i>Bifidobacterium longum</i> subsp. <i>longum</i> JCM7052.","authors":"Yuki Sasaki,&nbsp;Yumi Uchimura,&nbsp;Kanefumi Kitahara,&nbsp;Kiyotaka Fujita","doi":"10.5458/jag.jag.JAG-2021_0004","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2021_0004","url":null,"abstract":"<p><p>We recently characterized a 3-<i>O</i>-α-D-galactosyl-α-L-arabinofuranosidase (GAfase) for the release of α-D-Gal-(1→3)-L-Ara from gum arabic arabinogalactan protein (AGP) in <i>Bifidobacterium longum</i> subsp. <i>longum</i> JCM7052. In the present study, we cloned and characterized a neighboring α-galactosidase gene (BLGA_00330; <i>blAga3</i>). It contained an Open Reading Frame of 2151-bp nucleotides encoding 716 amino acids with an estimated molecular mass of 79,587 Da. Recombinant BlAga3 released galactose from α-D-Gal-(1→3)-L-Ara, but not from intact gum arabic AGP, and a little from the related oligosaccharides. The enzyme also showed the activity toward blood group B liner trisaccharide. The specific activity for α-D-Gal-(1→3)-L-Ara was 4.27- and 2.10-fold higher than those for melibiose and raffinose, respectively. The optimal pH and temperature were 6.0 and 50 °C, respectively. BlAga3 is an intracellular α-galactosidase that cleaves α-D-Gal-(1→3)-L-Ara produced by GAfase; it is also responsible for a series of gum arabic AGP degradation in <i>B. longum</i> JCM7052.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"68 2","pages":"47-52"},"PeriodicalIF":1.1,"publicationDate":"2021-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/27/a8/JAG-68-047.PMC8367640.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39341348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
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