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Journal of applied glycoscience最新文献

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Characterization of Cell Wall α-1,3-Glucan-Deficient Mutants in Aspergillus oryzae Isolated by a Screening Method Based on Their Sensitivities to Congo Red or Lysing Enzymes. 基于刚果红和裂解酶敏感性筛选法分离的米曲霉细胞壁α-1,3-葡聚糖缺陷突变体的特性
IF 1.1
Journal of applied glycoscience Pub Date : 2017-08-20 eCollection Date: 2017-01-01 DOI: 10.5458/jag.jag.JAG-2017_004
Akira Yoshimi, Misa Hirama, Yasunobu Tsubota, Kazuyoshi Kawakami, Silai Zhang, Katsuya Gomi, Keietsu Abe
{"title":"Characterization of Cell Wall α-1,3-Glucan-Deficient Mutants in <i>Aspergillus oryzae</i> Isolated by a Screening Method Based on Their Sensitivities to Congo Red or Lysing Enzymes.","authors":"Akira Yoshimi,&nbsp;Misa Hirama,&nbsp;Yasunobu Tsubota,&nbsp;Kazuyoshi Kawakami,&nbsp;Silai Zhang,&nbsp;Katsuya Gomi,&nbsp;Keietsu Abe","doi":"10.5458/jag.jag.JAG-2017_004","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2017_004","url":null,"abstract":"<p><p>We previously reported that sensitivity to Congo Red (CR) or Lysing Enzymes (LE) is affected by the loss of cell-wall α-1,3-glucan (AG) in <i>Aspergillus nidulans</i>. We found that the amount of CR adsorbed to AG was significantly less than the amount adsorbed to β-1,3-glucan (BG) or chitin, suggesting that loss of cell-wall AG would increase exposure of BG on the cell surface, and thereby increase the sensitivity to CR. Generally, fungal BGs are known as biological response modifiers because of their recognition by Dectin-1 receptors in human immune systems. Therefore, isolation of AG-deficient mutants in <i>Aspergillus oryzae</i> has been used in the Japanese fermentation industry to create strains with increased ability to promote immune responses. Here, we aimed to isolate AG-deficient strains by mutagenizing <i>A. oryzae</i> conidia with chemical mutagens. Based on the increased sensitivity to CR in AG-deficient strains of <i>A. nidulans</i> and <i>A. oryzae</i>, we established a screening method for isolation of AG-deficient strains. Several candidate AG-deficient mutants of <i>A. oryzae</i> were isolated using the screening method; these strains showed increased sensitivity to CR and/or LE. Cytokine production was increased in the dendritic cells co-incubated with germinated conidia of the AG-deficient mutants. Furthermore, according to a Dectin-1 NFAT (nuclear factor of activator T cells)-GFP (green fluorescent protein) reporter assay, Dectin-1 response levels in the AG-deficient mutants were higher than those in wild-type <i>A. oryzae</i>. These results suggest that we successfully isolated AG-deficient mutants of <i>A. oryzae</i> with immunostimulatory effects.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"64 3","pages":"65-73"},"PeriodicalIF":1.1,"publicationDate":"2017-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5458/jag.jag.JAG-2017_004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39280804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Progress in Research and Development of Potato Staple Food Processing Technology. 马铃薯主食加工技术的研究与开发进展。
IF 1.1
Journal of applied glycoscience Pub Date : 2017-08-20 eCollection Date: 2017-01-01 DOI: 10.5458/jag.jag.JAG-2016_017
Taihua Mu, Hongnan Sun
{"title":"Progress in Research and Development of Potato Staple Food Processing Technology.","authors":"Taihua Mu,&nbsp;Hongnan Sun","doi":"10.5458/jag.jag.JAG-2016_017","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2016_017","url":null,"abstract":"<p><p>In 2014, potato production in China amounted to 96 million tons, which was the highest in the world. As one of the most important nutritional foods in the world, potato is rich in starch, dietary fiber, vitamins, minerals, <i>etc</i>. Potatoes stand barren environment, drought, saline, and alkaline environment, and cold weather, with a short growing season. These features make them the best rain-fed crops suitable for production even when the annual rainfall is below 400 mm. In 2013, the Chinese Ministry of Agriculture suggested a potato staple food strategy using potatoes to make Chinese traditional staple foods such as steamed bread, noodles, <i>etc</i>. Our research group carried out a study on processing technology of potato staple food, especially fermented staple food. Some new processing technologies of potato staple food have been investigated and developed. The aim of this paper is to give an overview of the possible effects of adding potato flour in the dough and of the microstructure characteristics, technological parameters, total polyphenol content, and antioxidant activity of staple foods. We also systematically describe the processing technology of potato staple foods, which may be of great importance in promoting further expansion of the potato-processing industry and increasing the economic benefit of the companies.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"64 3","pages":"51-64"},"PeriodicalIF":1.1,"publicationDate":"2017-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5458/jag.jag.JAG-2016_017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39280803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
The Effect of Heat-acid Treatment on the Formation of Resistant Starch and the Estimated Glycemic Index in Potatoes. 热酸处理对马铃薯中抗性淀粉的形成和估计血糖生成指数的影响
IF 1.1
Journal of applied glycoscience Pub Date : 2017-08-20 eCollection Date: 2017-01-01 DOI: 10.5458/jag.jag.JAG-2017_001
Eri Udagawa, Hiroko Matsuda, Mamiko Tanaka, Takaaki Shirai
{"title":"The Effect of Heat-acid Treatment on the Formation of Resistant Starch and the Estimated Glycemic Index in Potatoes.","authors":"Eri Udagawa, Hiroko Matsuda, Mamiko Tanaka, Takaaki Shirai","doi":"10.5458/jag.jag.JAG-2017_001","DOIUrl":"10.5458/jag.jag.JAG-2017_001","url":null,"abstract":"<p><p>Potatoes are generally regarded as high glycemic index (GI) foods. Resistant starch (RS) comprises the starch fraction that is not absorbed in the small intestine, thus controlling the glucose level and improving the intestinal environment. In this study, an analysis of the formation of RS of potato starch samples under different acetic acid-thermal treatment conditions was conducted. Additionally, the relationship between the rates of starch digestion, estimated GI (eGI), and the RS content was evaluated by employing <i>in vitro</i> enzymatic models. Compared with control samples, the RS content in the cold-stored samples after acid-boiling was higher, whereas that of samples after heating at 120 °C with acetic acid was decreased. The eGI was negatively correlated with the RS content in potatoes. Cold store after acid-boiling was effective in increasing the RS content. Furthermore, low eGI values may have resulted from higher levels of RS in potatoes.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"64 3","pages":"75-80"},"PeriodicalIF":1.1,"publicationDate":"2017-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1d/6f/JAG-64-075.PMC8056890.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39280805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production of Gentiobiose from Hydrothermally Treated Aureobasidium pullulans β-1,3-1,6-Glucan. 水热处理普鲁兰金黄色小孢子草生产龙胆糖β- 1,3,6 -葡聚糖。
IF 1.1
Journal of applied glycoscience Pub Date : 2017-05-20 eCollection Date: 2017-01-01 DOI: 10.5458/jag.jag.JAG-2017_002
Katsuki Hirabayashi, Yoshiya Tashiro, Nobuhiro Kondo, Sachio Hayashi
{"title":"Production of Gentiobiose from Hydrothermally Treated <i>Aureobasidium pullulans</i> β-1,3-1,6-Glucan.","authors":"Katsuki Hirabayashi,&nbsp;Yoshiya Tashiro,&nbsp;Nobuhiro Kondo,&nbsp;Sachio Hayashi","doi":"10.5458/jag.jag.JAG-2017_002","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2017_002","url":null,"abstract":"<p><p>We report production of the functional disaccharide gentiobiose β-D-Glc<i>p</i>-(1→6)-D-Glc by a hydrolysis reaction of hydrothermally treated <i>Aureobasidium pullulans</i> β-1,3-1,6-glucan as the substrate and Kitalase as the enzyme. Gentiobiose was produced over the pH range 4-6 and the concentration of gentiobiose produced decreased above pH 7. The maximum value of gentiobiose production was unaffected by the enzyme concentration. The maximum concentration of gentiobiose produced was dependent on the substrate concentration whereas the maximum ratio of gentiobiose to glucose was not. The production of gentiobiose from yeast β-1,3-1,6-glucan was lower than that from <i>A. pullulans</i> β-1,3-1,6-glucan.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"64 2","pages":"33-37"},"PeriodicalIF":1.1,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5458/jag.jag.JAG-2017_002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39289242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of Amylose Nanogels and Microgels Containing Ionic Polysaccharides. 含离子多糖直链淀粉纳米凝胶和微凝胶的表征。
IF 1.1
Journal of applied glycoscience Pub Date : 2017-05-20 eCollection Date: 2017-01-01 DOI: 10.5458/jag.jag.JAG-2016_012
Shiho Suzuki, Junichiro Nishioka, Shinichi Kitamura
{"title":"Characterization of Amylose Nanogels and Microgels Containing Ionic Polysaccharides.","authors":"Shiho Suzuki,&nbsp;Junichiro Nishioka,&nbsp;Shinichi Kitamura","doi":"10.5458/jag.jag.JAG-2016_012","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2016_012","url":null,"abstract":"<p><p>We prepared and characterized amylose nanogels containing ionic polysaccharides which we used were 4-<i>O</i>-methyl-D-glucurono-D-xylan (GX), alginate, xanthan, and chitosan. Gelation under a shear force followed by a wet pulverization leads to the formation of hybrid nanogels. The resultant nanogels were characterized by particle size analysis, zeta-potential measurement and atomic force microscopy (AFM). Wet pulverization under a pressure of 200 MPa reduced the particle size of the gels from 20-26 μm to 240-670 nm. Zeta potential measurement showed that the ionic polysaccharides increased surface charges of the amylose gels. AFM observations showed the network consisting of submicron size amylose-polysaccharide nano fibrils. The fibrils containing GX were dispersed uniformly, while those containing only amylose were partly aggregated.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"64 2","pages":"21-25"},"PeriodicalIF":1.1,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/dd/cf/JAG-64-021.PMC8056922.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39289240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Insertion of a Loop Structure into the "Loopless" GH19 Chitinase from Bryum coronatum. 在冠状Bryum“无环”GH19几丁质酶中插入环状结构。
IF 1.1
Journal of applied glycoscience Pub Date : 2017-05-20 eCollection Date: 2017-01-01 DOI: 10.5458/jag.jag.JAG-2016_015
Shoko Takenaka, Takayuki Ohnuma, Tamo Fukamizo
{"title":"Insertion of a Loop Structure into the \"Loopless\" GH19 Chitinase from <i>Bryum coronatum</i>.","authors":"Shoko Takenaka,&nbsp;Takayuki Ohnuma,&nbsp;Tamo Fukamizo","doi":"10.5458/jag.jag.JAG-2016_015","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2016_015","url":null,"abstract":"<p><p>Chitinases belonging to the GH19 family have diverse loop structure arrangements. A GH19 chitinase from rye seeds (RSC-c) has a full set of (six) loop structures that form an extended binding cleft from -4 to +4 (\"loopful\"), while that from moss (BcChi-A) lacks several loops and forms a shortened binding cleft from -2 to +2 (\"loopless\"). We herein inserted a loop involved in sugar residue binding at subsites +3 and +4 of RSC-c (Loop-II) into BcChi-A (BcChi-A+L-II), and the thermal stability and enzymatic activity of BcChi-A+L-II were then characterized and compared with those of BcChi-A. The transition temperature of thermal unfolding decreased from 77.2 ˚C (BcChi-A) to 63.3 ˚C (BcChi-A+L-II) by insertion of Loop-II. Enzymatic activities toward the chitin tetramer (GlcNAc)<sub>4</sub> and the polymeric substrate glycol chitin were also suppressed by the Loop-II insertion to 12 and 9 %, respectively. The Loop-II inserted into BcChi-A was found to be markedly flexible and disadvantageous for protein stability and enzymatic activity.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"64 2","pages":"39-42"},"PeriodicalIF":1.1,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5458/jag.jag.JAG-2016_015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39289243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Efficient Alkaline Isomerization of Lactose to Lactulose in the Presence of an Organogermanium Compound. 有机锗化合物存在时乳糖到乳果糖的高效碱性异构化。
IF 1.1
Journal of applied glycoscience Pub Date : 2017-05-20 eCollection Date: 2017-01-01 DOI: 10.5458/jag.jag.JAG-2016_018
Takae Nagasawa, Katsuyuki Sato, Takafumi Kasumi
{"title":"Efficient Alkaline Isomerization of Lactose to Lactulose in the Presence of an Organogermanium Compound.","authors":"Takae Nagasawa, Katsuyuki Sato, Takafumi Kasumi","doi":"10.5458/jag.jag.JAG-2016_018","DOIUrl":"10.5458/jag.jag.JAG-2016_018","url":null,"abstract":"<p><p>Lactulose, a disaccharide widely used in pharmaceuticals and functional foods, is produced by lactose isomerization. Lactose and lactulose have an aldose-ketose relationship. Less than 25 % conversion of lactose into lactulose is achieved using the Lobry de Bruyn-Alberda van Ekenstein transformation with heating, whereas the conversion is increased to 80 % by the addition of an approximately equimolar concentration of the organogermanium compound 3-(trihydroxygermyl)propanoic acid (THGP) to the reaction mixture. To further understand this phenomenon, in this study, we analyzed the affinity between THGP and sugar isomers using <sup>1</sup>H nuclear magnetic resonance spectroscopy. For the dimethyl derivative of THGP with lactose and lactulose, the complex formation ratios at 0.1 M (1:1 mixing ratio) were 14 and 59 %, respectively, with complex formation constants of 1.8 and 43 M<sup>-1</sup>, respectively. The complex formation capacity was approximately 24-fold higher for lactulose than for lactose. Moreover, THGP is considered to protect lactulose from alkaline degradation, resulting in high production yield of lactulose. Therefore, we concluded that high affinity for the isomerization product may promote isomerization and that promotion of sugar isomerization using an organogermanium compound is an effective method for converting lactose to lactulose.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"64 2","pages":"27-32"},"PeriodicalIF":1.1,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d2/ae/JAG-64-027.PMC8056929.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39289241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An α2,3-Linked Sialylglycopolymer as a Multivalent Glycoligand Against Avian and Human Influenza Viruses. 一种 α2,3-连接的 Sialylglycopolymer 作为多价糖配体，可对抗禽流感和人类流感病毒。
IF 1.1
Journal of applied glycoscience Pub Date : 2017-05-20 eCollection Date: 2017-01-01 DOI: 10.5458/jag.jag.JAG-2017_003
Tomonari Tanaka, Yiting Zhou, Chihiro Tamoto, Yuuki Kurebayashi, Tadanobu Takahashi, Takashi Suzuki
{"title":"An α2,3-Linked Sialylglycopolymer as a Multivalent Glycoligand Against Avian and Human Influenza Viruses.","authors":"Tomonari Tanaka, Yiting Zhou, Chihiro Tamoto, Yuuki Kurebayashi, Tadanobu Takahashi, Takashi Suzuki","doi":"10.5458/jag.jag.JAG-2017_003","DOIUrl":"10.5458/jag.jag.JAG-2017_003","url":null,"abstract":"<p><p>A glycopolymer bearing α2,3-linked sialyltrisaccharides was synthesized by living radical polymerization using a glycomonomer prepared by a protecting-group-free process, direct azidation of the free sialyllactose, and subsequent azide-alkyne cycloaddition. The prepared glycopolymer with pendant 3´-sialyllactose moieties strongly interacted with both avian and human influenza viruses analyzed by the hemagglutination inhibition assay and the quartz crystal microbalance method.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"64 2","pages":"43-48"},"PeriodicalIF":1.1,"publicationDate":"2017-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ed/80/JAG-64-043.PMC8056908.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39280802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of Three Fungal Isomaltases Belonging to Glycoside Hydrolase Family 13 That Do not Show Transglycosylation Activity. 不显示转糖基化活性的三种属于糖苷水解酶家族13的真菌异麦芽糖酶的表征。
IF 1.1
Journal of applied glycoscience Pub Date : 2017-02-20 eCollection Date: 2017-01-01 DOI: 10.5458/jag.jag.JAG-2016_009
Hiroki Eisawa, Shun Ogawa, Nobuhiro Yamazaki, Kohki Maekawa, Takahiro Yamaguchi, Shota Sato, Kazuma Shiota, Takashi Yoshida
{"title":"Characterization of Three Fungal Isomaltases Belonging to Glycoside Hydrolase Family 13 That Do not Show Transglycosylation Activity.","authors":"Hiroki Eisawa,&nbsp;Shun Ogawa,&nbsp;Nobuhiro Yamazaki,&nbsp;Kohki Maekawa,&nbsp;Takahiro Yamaguchi,&nbsp;Shota Sato,&nbsp;Kazuma Shiota,&nbsp;Takashi Yoshida","doi":"10.5458/jag.jag.JAG-2016_009","DOIUrl":"https://doi.org/10.5458/jag.jag.JAG-2016_009","url":null,"abstract":"<p><p>α-1,6-Glucosidase (isomaltase) belongs to glycoside hydrolase (GH) families 13 and 31. Genes encoding 3 isomaltases belonging to GH family 13 were cloned from filamentous fungi, <i>Aspergillus oryzae</i> (<i>agl1</i>), <i>A. niger</i> (<i>agdC</i>),and <i>Fusarium oxysporum</i> (<i>foagl1</i>), and expressed in <i>Escherichia coli</i>. The enzymes hydrolyzed isomaltose and α-glucosides preferentially at a neutral pH, but did not recognize maltose, trehalose, and dextran. The activity of AgdC and Agl1 was inhibited in the presence of 1 % glucose, while Foagl1 was more tolerant to glucose than the other two enzymes were. The three fungal isomaltases did not show transglycosylation when isomaltose was used as the substrate and a similar result was observed for AgdC and Agl1 when <i>p</i>-nitrophenyl-α-glucoside was used as the substrate.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"64 1","pages":"9-13"},"PeriodicalIF":1.1,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2b/4b/JAG-64-009.PMC8056888.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39289238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Structural Analysis of Novel Low-Digestible Sucrose Isomers Synthesized from D-Glucose and D-Fructose by Thermal Treatment. d -葡萄糖和d -果糖热合成新型低消化蔗糖异构体的结构分析。
IF 1.1
Journal of applied glycoscience Pub Date : 2017-02-20 eCollection Date: 2017-01-01 DOI: 10.5458/jag.jag.JAG-2016_011
Akira Yamamori, Yusuke Takata, Eri Fukushi, Jun Kawabata, Hideki Okada, Naoki Kawazoe, Keiji Ueno, Shuichi Onodera, Norio Shiomi
{"title":"Structural Analysis of Novel Low-Digestible Sucrose Isomers Synthesized from D-Glucose and D-Fructose by Thermal Treatment.","authors":"Akira Yamamori, Yusuke Takata, Eri Fukushi, Jun Kawabata, Hideki Okada, Naoki Kawazoe, Keiji Ueno, Shuichi Onodera, Norio Shiomi","doi":"10.5458/jag.jag.JAG-2016_011","DOIUrl":"10.5458/jag.jag.JAG-2016_011","url":null,"abstract":"<p><p>The synthesis of the saccharide β-D-fructopyranosyl-(2→6)-D-glucopyranose, which was isolated from Super Ohtaka<sup>®</sup>, has recently been reported. During the synthesis of this saccharide, the formation of two novel saccharides from D-glucose and D-fructose was observed. The present study aimed to confirm the structures of the two disaccharides synthesized from D-glucose and D-fructose by thermal treatment. Furthermore, various properties of the saccharides were investigated. Both saccharides were isolated from the reaction mixture by carbon-Celite column chromatography and an HPLC system and were determined to be novel sucrose-isomers, β-D-fructopyranosyl-(2↔1)-β-D-glucopyranoside (1) and β-D-fructofuranosyl-(2↔1)-β-D-glucopyranoside (2), by MALDI-TOF MS and NMR analyses. Both saccharides showed low digestibility <i>in vitro</i>, and the sweetness of saccharide 2 was 0.45 times that of sucrose.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"64 1","pages":"15-19"},"PeriodicalIF":1.1,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5458/jag.jag.JAG-2016_011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39289239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
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