Journal of Analytical Methods in Chemistry最新文献

{"title":"The Effect of Hemolysis on Biochemical Tests: Determining Interference Cutoffs and Managing Hemolytic Samples.","authors":"Babak Shirazi Yeganeh, Neda Soleimani, Saeedeh Zare, Sahand Mohammadzadeh, Farzaneh Amiri, Mohammad Javad Esmaeili, Davoud Soleimani, Soodabeh Khoshnyat","doi":"10.1155/jamc/9307894","DOIUrl":"10.1155/jamc/9307894","url":null,"abstract":"<p><strong>Background: </strong>Hemolysis is a common source of interference in biochemical tests, potentially leading to significant errors in clinical decision-making and patient management. This study aimed to evaluate the impact of varying degrees of hemolysis on routine biochemical analytes and to find a local hemolytic index (HIX) cutoff for safe reporting.</p><p><strong>Methods: </strong>This experimental study was conducted on 30 serum samples from healthy individuals with normal biochemical profiles. Baseline concentrations of 20 routine analytes were measured using a DIRUI CS-1200 analyzer. To simulate hemolysis, increasing concentrations of autologous hemolysate were added to each sample, creating three grades of hemolysis based on HIX (mild, moderate, and severe). All analytes were remeasured, and the absolute and relative biases were calculated against baseline levels. These biases were then compared with the CLIA-defined total allowable error (TEa) to obtain the safe reporting cutoff for each analyte.</p><p><strong>Results: </strong>All analytes showed a positive bias with increasing hemolysis, though the magnitude of interference varied significantly. LDH, uric acid, AST, and total bilirubin, in that order, were more impacted by hemolysis. In mild hemolysis (HIX < 0.5), none of the analyte biases exceeded TEa limits (<i>p</i> value < 0.05). In moderate and severe hemolysis, however, most exceeded acceptable limits. Based on these results, a HIX cutoff of 0.5 was regarded for reporting mildly hemolyzed samples.</p><p><strong>Conclusion: </strong>Hemolysis interference is both analyte- and system-dependent. Laboratories are recommended to conduct similar research tailored to their local laboratory settings and population to improve analytical quality and reduce unnecessary specimen rejection.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9307894"},"PeriodicalIF":2.1,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12946476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147326036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and Mechanism Prediction of Active Components in Fuganlin Oral Liquid for Respiratory Tract Infections Using UPLC-Q-TOF-MS and Network Pharmacology.","authors":"Mengyue Zhang, Feng Han, Mingxuan Yang, Zhishan Ye, Ying Cui, Yuefei Wang, Jing Yang, Xin Chai","doi":"10.1155/jamc/3075224","DOIUrl":"10.1155/jamc/3075224","url":null,"abstract":"<p><p>Fuganlin oral liquid (FOL) has been clinically employed for the treatment of pediatric qi deficiency colds, manifesting symptoms such as fever, cough, asthma, and sore throat. However, the chemical composition and bioactive components of FOL have not been clearly elucidated. In this study, a comprehensive qualitative analysis of FOL was conducted utilizing ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry in conjunction with network pharmacology. A total of 124 chemical components were tentatively characterized, comprising flavonoids, phenolic acids, saponins, coumarins, and others. Among these, 43 compounds were unequivocally identified by comparison with authentic reference standards. Furthermore, network pharmacology analysis revealed that the ingredients of FOL exhibited anti-inflammatory properties and demonstrated potential efficacy in relieving cough and asthma associated with respiratory tract infections. Collectively, this study provides the first comprehensive characterization of the chemical composition in FOL and explores the potential pharmacological mechanisms of its bioactive constituents, thereby providing scientific support for quality control standards and clinical applications.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"3075224"},"PeriodicalIF":2.1,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12894422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Making of the Vindolanda Wooden Writing Tablets: A Noninvasive Multianalytical Protocol for the Characterisation of Black Roman Inks.","authors":"Giovanna Vasco, Joanne Dyer, Richard Hobbs, Caroline R Cartwright, Alessandro Buccolieri","doi":"10.1155/jamc/5142007","DOIUrl":"10.1155/jamc/5142007","url":null,"abstract":"<p><p>Within the 'Making History' project, the British Museum investigated the materiality of the Vindolanda ink writing tablets for the first time, with a particular focus on the possible differentiation of the ink sources employed. Thanks to the application of complementary scientific techniques, it was possible to develop an analytical protocol for the documentation of the ink writing from the palaeographic and conservation points of view and the characterisation of their manufacture. From the macroscopic to the microscopic scale, the use of imaging techniques (MBI, SWIR and digital microscope) highlighted the unique features of the different handwriting styles and allowed the identification of the ink typology. Raman spectroscopy and elemental analyses (XRF and SEM-EDX) were then applied to gain information about the chemical-physical nature of the inks. The main achievement was the possibility to differentiate the sources of carbon-based inks using Raman spectroscopy and multivariate analysis. It was possible to examine ink production at the edge of the Roman Empire, comparing it with the ancient literature and the different practices arising in the Mediterranean area.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"5142007"},"PeriodicalIF":2.1,"publicationDate":"2026-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12884006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146157309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive and Rapid Chemical Profiling of <i>Cirsium japonicum</i> DC Utilizing UHPLC-Q-Orbitrap MS With Parallel Reaction Monitoring.","authors":"Jia-Yi Wang, Yu-Feng Zou, Kai-Lin Li, Yi-Fan Chen, Ling Liu, Shu-Sen Wu, Hui Li","doi":"10.1155/jamc/5782099","DOIUrl":"10.1155/jamc/5782099","url":null,"abstract":"<p><p><i>Cirsium japonicum</i>, a traditional medicinal plant, has been widely used for its therapeutic properties in treating various ailments. However, a comprehensive analysis of its chemical composition remains limited, hindering a full understanding of its chemical basis and pharmacological activities. This study aims to identify and characterize the chemical constituents of <i>C. japonicum</i> using ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap MS) and parallel reaction monitoring (PRM). A total of 94 compounds were identified, including 57 organic acids, 25 flavonoids, 3 phenylpropanoids, and 9 other components, with chlorogenic acid dominating the organic acid fraction. Organic acids and caffeic acid, among organic acid compounds, exhibit hemostatic effects based on prior evidence. The UHPLC-Q-Exactive Orbitrap MS-based approach provided a detailed chemical profile of <i>C. japonicum</i>, which could facilitate a deeper understanding of its medicinal properties and guide future pharmacological studies. The identified compounds can be used as potential biomarkers for the standardization and quality control of medicinal products of <i>C. japonicum</i>.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"5782099"},"PeriodicalIF":2.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12859162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Detection of Organochlorine Pesticide Residues in Sesame Seeds (<i>Sesamum indicum L</i>.) Using Advanced GC-MS/MS Techniques.","authors":"Kero Assefa Ago, Molla Tefera","doi":"10.1155/jamc/8312847","DOIUrl":"10.1155/jamc/8312847","url":null,"abstract":"<p><p>Considering the economic and edible values of sesame seeds, it is important to monitor sesame seed for the safety of consumers and for international trade as it helps the country government and suppliers as products to gain market acceptance more effectively. The objective of this study was to determine selected organochlorine pesticide (OCP) residues in sesame seeds. Quick, easy, cheap, efficient, rugged, and safe method followed by gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) was used for the rapid separation and determination of 20 OCPs in sesame seeds. Acetonitrile in combination with 1% glacial acetic acid was used as an extraction solvent. Primary secondary amine, graphitized carbon black, and octadecylsilane in QuEChERS kit were used for d-SPE clean up before GC-MS/MS analysis. The GC-MS/MS analysis was evaluated in terms of linearity, recovery, and precision. The calibration curves were obtained for all analytes and displayed good linearity over the selected concentration range with regression coefficients (<i>r</i> ²) ≥ 0.999. The recoveries for spiked analytes in sesame seed samples were ranged from 93.58 to 115.81 with RSDs lower than 1%. The LOD and LOQ for all investigated pesticides were in the range of 0.05-0.88 μg/kg and from 0.16 to 2.93 μg/kg, respectively.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"8312847"},"PeriodicalIF":2.1,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12817196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Hollow Fiber-Supported ZIF-8@GO Reinforced Sol-Gel for Preconcentration of Paraquat Before Determination by UV-Vis Spectrophotometry\".","authors":"","doi":"10.1155/jamc/9874058","DOIUrl":"10.1155/jamc/9874058","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/jamc/6883692.].</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9874058"},"PeriodicalIF":2.2,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12812251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Phenolic- and Saponin-Enriched Extraction From <i>Pandanus tectorius</i> Fruit Using Box-Behnken Design and Evaluation of Their Bioactivities.","authors":"Do Hoang Giang, Nguyen Hai Dang, Tran Thi Thu Phuong, Le Thanh Huong, Nguyen Thu Uyen, Nguyen Thi Luyen, Nguyen Thi Thu Thuy, Hoang Le Tuan Anh, Nguyen Ngoc Tung, Nguyen Tien Dat","doi":"10.1155/jamc/5539843","DOIUrl":"10.1155/jamc/5539843","url":null,"abstract":"<p><p><i>Pandanus tectorius</i> fruits are a promising but underutilized source of bioactive constituents. We optimized extraction conditions for phenolic- and saponin-enriched fractions using Box-Behnken/response surface methodology across ethanol concentration, temperature, solvent-to-material ratio, and time and then evaluated antioxidant and anti-inflammatory activities. Total phenolic content (TPC; Folin-Ciocalteu, 760 nm) and total saponin content (TSC; vanillin-sulfuric acid, 560 nm) served as responses for model fitting (<i>R</i> ² > 0.96), validation, and multiresponse optimization that yielded seven distinct optimums targeting different extract profiles. Phenolic-rich extracts showed potent DPPH and hydroxyl radical scavenging, whereas saponin-rich extracts more strongly inhibited LPS-induced nitric oxide in RAW 264.7 cells; excessive saponin enrichment, however, coincided with cytotoxicity. These results demonstrate that tuned extraction can deliver purpose-built extracts for antioxidant or anti-inflammatory applications, supporting the valorization of <i>P. tectorius</i> as a natural source for functional and nutraceutical ingredients.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"5539843"},"PeriodicalIF":2.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12767014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145911523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Evaluation of Decoction Pieces of <i>Fagopyri Dibotryis Rhizoma</i> Based on HPLC Fingerprint, Q-TOF-MS/MS, and Chemical Pattern Recognition Qualitative Analysis Combined With Multicomponent Quantitative Analysis.","authors":"Yi Cao, Meng He, Yaru Feng, Yujia Li, Nan Qin, Zhihua Dou","doi":"10.1155/jamc/2328049","DOIUrl":"10.1155/jamc/2328049","url":null,"abstract":"<p><p><i>Fagopyri Dibotryis Rhizoma</i> (FDR), the dried rhizome of <i>Fagopyrum dibotrys</i> (D. Don) Hara (<i>F. dibotrys</i>), is a famous herbal drug with a long application history in China. Recently, FDR and its preparations have attracted wide attention due to their therapeutic values for coronavirus disease 2019 (COVID-19) and COVID-19-related acute lung injury. There is a significant difference in quality among FDRs from different habitats, which can seriously affect the clinical efficacy. The original medicinal materials can only be used in the clinic after being processed into decoction pieces, but there is currently a lack of comprehensive quality evaluation of FDR decoction pieces prepared using FDRs from different origins. In this study, the HPLC fingerprints of 23 batches of FDR decoction pieces prepared using FDRs from 8 provinces such as Yunnan and Guizhou in China were established; 47 common peaks in these fingerprints were marked, among which, 80 components were identified by Q-TOF-MS/MS, including 32 tannins, 17 phenols, 12 flavonoids, 11 phenylpropanoid glycosides, 3 amino acids, 2 organic acids, 1 terpenoid, 1 alkaloid, and 1 other component; the chemical pattern recognition analysis, including hierarchical cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis, was conducted by using the quantified peak areas of the common peaks as variables; and the contents of 4 tannins such as procyanidin B1, procyanidin B3, procyanidin C1, and procyanidin A2, 3 phenolics such as gallic acid, protocatechuic acid, and protocatechualdehyde, and 3 flavonoids such as catechin, epicatechin, and epicatechin gallate were determined in 23 batches of FDR decoction pieces. The results indicate that there is a significant difference in the quality between the decoction pieces prepared using FDRs from Yunnan and Guizhou and those prepared using FDRs from 6 other provinces, and the former have a better quality compared with the latter.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"2328049"},"PeriodicalIF":2.2,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12643668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145604252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Characteristics of Soil Nonvolatile Metabolites From Geographical Locations in Guizhou Using UPLC Fingerprint Profiling.","authors":"Jie Zhang, Jinxiu Luo, Wei Jiang, Ruijuan Zhao, Yechun Lin, Dongqing Xu, Wenjing Zhu, Weichang Gao, Kai Cai","doi":"10.1155/jamc/5197541","DOIUrl":"10.1155/jamc/5197541","url":null,"abstract":"<p><p>Soil metabolites are not only the integrated expression of soil biological activities but also the pivotal drivers of biogeochemical cycling, thereby significantly influencing the formation of crop quality. This study established an improved ultra-performance liquid chromatography (UPLC) fingerprint with chemometrics for characterizing the nonvolatile metabolite profiles of soils from geographical locations defined by different tobacco flavor styles in Guizhou. The homogenization extraction and then UPLC analysis were selected as the optimal system due to their superior repeatability and reproducibility, with intraday and interday RSD% of the common peaks (retention time and peak area) below 2.75%. The fingerprint profiling was established using 18 soil samples from three locations, namely the honey-sweet region I, II, and the fresh-sweet region. Thirty common peaks were identified, with similarities ranging from 0.809 to 0.956. Then, the common peaks were subjected to chemometrics analysis. These results indicated that significant differences were observed by principal component analysis (PCA), and 17 characteristic metabolites were viewed as primarily discriminatory by partial least squares discriminant analysis (PLS-DA). The total content of characteristic metabolites followed a trend of honey-sweet region II > honey-sweet region I > fresh-sweet region, with individual metabolites generally higher in the honey-sweet region. Finally, external validation using the hierarchical cluster analysis (HCA) and Fisher discriminant analysis (FDA) model accurately classified four soil samples, further confirming the representativeness of the characteristic metabolites. This study supplies a theoretical foundation to understand the relationship between tobacco flavor formation and soil metabolism, showing great potential applications in agricultural research.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"5197541"},"PeriodicalIF":2.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12530930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145329048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reliable and Cost-Effective QuEChERS-UHPLC-MS/MS Method for the Simultaneous Analysis of Ten Mycotoxins in Dendrobium Officinale: A Study on Food-Medicine Homology Quality Control.","authors":"Zejun Wang, Qingcheng Wang, Fei Xu, Linhua Wang, Jingjing Liang, Qing Shen, Shunyuan Guo","doi":"10.1155/jamc/8995925","DOIUrl":"10.1155/jamc/8995925","url":null,"abstract":"<p><p>This study developed a method for simultaneously detecting 10 mycotoxins in Dendrobium officinale using the QuEChERS technique combined with ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The method was optimized for sample purification, pretreatment, chromatographic conditions, and mass spectrometric settings. It effectively addressed the matrix effects from impurities like pigments and cellulose. The validation of the method showed good linearity (<i>R</i> ² > 0.990), with limits of detection (LODs) ranging from 0.23 to 8.61 μg kg^-1 and limits of quantification (LOQs) from 0.77 to 28.7 μg kg^-1. Average recoveries for the 10 mycotoxins ranged from 77.9% to 98.5%, with relative standard deviations (RSD) between 2.26% and 8.28%. The method demonstrated high accuracy, precision, and suitability for large-scale screening of mycotoxins in Dendrobium officinale. When applied to 84 samples, the contamination rate was 2.38%, with the main contaminants being AFB1, ZEN, and AFG1. This method provides a reliable, cost-effective approach for detecting mycotoxin contamination in traditional Chinese medicine.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"8995925"},"PeriodicalIF":2.2,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}