Journal of Analytical Methods in Chemistry最新文献

{"title":"Tracing Hydrazine in Pharmaceuticals: Toxicological Concerns, Extraction Approaches, and Analytical Method Development.","authors":"Buhlebenkosi Mercy Donga, Rimadani Pratiwi, Aliya Nur Hasanah","doi":"10.1155/jamc/9609425","DOIUrl":"https://doi.org/10.1155/jamc/9609425","url":null,"abstract":"<p><p>Hydrazine (N₂H₄) is a synthetic reagent that is used in the manufacturing of various active pharmaceutical ingredients (APIs) such as isoniazid, hydralazine, and carbidopa. The major concerns associated with its presence in medications as a residual reagent or degradation product arise because of its ability to damage the genetic material, cause mutations, and potentially trigger cancer. It has been classified under Class 2 genotoxic impurity (GTI), based on the ICH M7 guideline. Its multitissue toxicities including hepatotoxicity, neurotoxicity, and hematotoxicity have led to tight regulatory limits with the EMA threshold of toxicological concern (TTC) at 1.5 µg/day. Detecting hydrazine in trace concentrations is challenging because of its low molecular weight, high polarity, absence of chromophores, and chemical instability. In order to provide a unified framework for impurity profiling, we combine hydrazine's toxicological profiles, formation pathways, and analytical advancements. Recent advances on the extraction, derivatization, and analytical determination of hydrazine to improve its stability and detectability in pharmaceutical matrices ranging from 2020 to 2025 were collected. Comparative evaluation of the methodologies reveals that combining chemical derivatization with hyphenated techniques delivers the highest sensitivity. GC-MS/MS stands out as the gold standard, providing exceptionally low detection limits at the sub-ppm level with high precision and reliability, while UHPLC-fluorescence serves as an alternative with considerable sensitivity for detecting hydrazine. Gaps in method harmonization, reference standard availability, and validation for complex formulations still remain therefore future work should focus more on standardized analytical methods, development of greener methods of derivatization, as well as use of high-resolution detection systems.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9609425"},"PeriodicalIF":2.1,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Phytochemical Profiles and Bioactivity-Constituent Relationships in Seven <i>Pandanus</i> Species From Vietnam.","authors":"Do Hoang Giang, Le Thanh Huong, Hoang Thuy Duong, Nguyen Thu Uyen, Nguyen Tien Dat, Nguyen Hai Dang","doi":"10.1155/jamc/6373690","DOIUrl":"10.1155/jamc/6373690","url":null,"abstract":"<p><p>The genus <i>Pandanus</i> comprises numerous tropical plant species that are widely used in traditional medicine and local diets, yet comprehensive comparative studies linking their phytochemical composition to biological activities remain limited, particularly for underexplored species. In this study, forty-five methanolic extracts obtained from seven <i>Pandanus</i> species (<i>Pandanus tectorius, Pandanus amaryllifolius, Pandanus odoratissimus, Pandanus affinis, Pandanus nanofrutex, Pandanus humilis,</i> and <i>Pandanus tonkinensis</i>) collected in Vietnam were systematically evaluated for their phytochemical profiles and bioactivities. Total phenolic (TPC), flavonoid (TFC), saponin (TSC), and alkaloid (TAC) contents were quantified, and antioxidant activities (DPPH and hydroxyl radical scavenging) together with <i>α</i>-amylase inhibitory effects were assessed. Pronounced interspecific variation, together with selected exploratory organ-related patterns, was observed, with <i>P. tectorius</i> exhibiting consistently high phenolic, flavonoid, and saponin contents and the strongest antioxidant activities, while <i>P. amaryllifolius</i> was distinguished by exceptionally high alkaloid levels. To elucidate the relationships between chemical constituents and bioactivities, partial least squares regression (PLSR) and Spearman's correlation analyses were applied. The PLSR models showed strong explanatory and predictive performance (<i>Q</i> ² = 0.889), indicating that phenolic content showed the strongest association with free radical scavenging activities, whereas saponin content was most strongly associated with <i>α</i>-amylase inhibition. These patterns were consistent with the Spearman correlation analysis and the PLS biplot. Overall, this study provides an integrated phytochemical-bioactivity framework for <i>Pandanus</i> species, highlights distinct associations between metabolite classes and the measured bioactivities, and identifies underexplored taxa as promising candidates for future phytochemical and pharmacological investigations.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"6373690"},"PeriodicalIF":2.1,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13676034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frontiers in Neurotransmitter Probes: Innovations and Perspectives in Norepinephrine Detection Technology.","authors":"Rufei Ma, Zhao Wang","doi":"10.1155/jamc/5515088","DOIUrl":"10.1155/jamc/5515088","url":null,"abstract":"<p><p>Norepinephrine (NE), the primary neurotransmitter of locus coeruleus projections, plays a crucial role in regulating arousal, emotion, memory, and stress responses. Its dysregulation is closely associated with various neurological disorders such as depression, Parkinson's disease, and epilepsy. However, conventional detection techniques such as microdialysis coupled with liquid chromatography are constrained by limited spatiotemporal resolution and inherent invasiveness, largely due to NE's low concentration, rapid metabolism, and complex distribution in vivo. These limitations make real-time, in situ dynamic monitoring particularly challenging. In recent years, fluorescence and bioluminescence probe technologies, founded on molecular recognition or self-assembly strategies, have enabled notable advances in the in situ detection and imaging of NE, owing to their superior spatiotemporal resolution, favorable biocompatibility, and versatile design. This review summarizes several representative studies in the field, covering probe design principles, response mechanisms, analytical performance, and biological applications. It also highlights emerging frontiers such as subcellular localization, in vivo dynamic imaging, millisecond-scale monitoring, multitarget cooperative recognition, and integrated diagnosis and therapy. These technologies have deepened our understanding of the spatiotemporal dynamics of NE in physiological and pathological processes, while also providing powerful tools for mechanistic research, early diagnosis, and precise treatment of neuropsychiatric disorders.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"5515088"},"PeriodicalIF":2.1,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13676032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Polyphenol Recovery From <i>Embelia ribes</i> Using MP-UAE, UAE, and MAE and In Silico Studies Against HCV Protease.","authors":"Faiza Imtiaz, Dildar Ahmed, Saira Zumar, Samiah H Al-Mijalli, Munawar Iqbal","doi":"10.1155/jamc/5570481","DOIUrl":"10.1155/jamc/5570481","url":null,"abstract":"<p><p><i>Embelia ribes</i> (ER) is well regarded in herbal medicine for its therapeutic properties, making the development of efficient methods to obtain its bioactive extracts of high yield essential. This study evaluated and optimized three extraction techniques, microwave-pretreated ultrasound-assisted extraction (MP-UAE), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE), to isolate bioactive compounds from ER seeds. Embelin, a primary bioactive, was quantified in the optimized extracts, while 13 bioactives, including embelin, were analyzed through in silico studies targeting HCV NS3 protease. Process optimization using response surface methodology (RSM) focused on key parameters: total phenolic content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity (DRSA), and ABTS radical scavenging activity (ARSA). Experimental results validated the models, with MP-UAE yielding the highest TPC (183.00 mg GAE/g DW), TFC (54.54 mg RE/g DW), DRSA (72.80%), and ARSA (81.33%). Optimized conditions included microwave power of 238 W, 48-min sonication, and a 19-mL/g solvent-to-plant ratio for MP-UAE. Embelin content, measured spectrophotometrically, was highest in MP-UAE at 39.98 ± 0.97 mg/g DW. Molecular docking using Maestro 12.5 identified amentoflavone (-8.991 kcal/mol), embelin (-8.152 kcal/mol), quercetin (-8.107 kcal/mol), and aloe-emodin (-8.014 kcal/mol) as top compounds, surpassing the standard drug boceprevir (-5.342 kcal/mol). This study establishes a green, efficient extraction method for ER seed bioactives, highlighting the antiviral potential of embelin and contributing to herb-based pharmaceutical advancements, particularly in antiviral drug development.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"5570481"},"PeriodicalIF":2.1,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13618570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149074603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction, Purification, Structural Elucidation, and Antioxidant Assessment of the Bioactive Polysaccharides From Xinjiang Dried Apricot.","authors":"Hongying Ma, Xiaotian Wang, Zhongyang Li, Huakun Ying, Cheng Ma, Shuailian Zhao, Jiangli Lin, Bin Wu","doi":"10.1155/jamc/2526753","DOIUrl":"10.1155/jamc/2526753","url":null,"abstract":"<p><strong>Background: </strong>Xinjiang apricots (<i>Prunus armeniaca</i> L.) are rich in bioactive polysaccharides with antioxidant, anti-inflammatory, and immunomodulatory properties. As natural antioxidants, such polysaccharides offer advantages over synthetic counterparts in mitigating ROS-related diseases. Their antioxidant depends on structural features, yet Xinjiang apricot polysaccharides remain poorly characterized. This study employs ultrasound-assisted enzymatic extraction, DEAE-52 purification, and multitechnique structural analysis to investigate these polysaccharides, aiming to establish their structure-activity relationship for potential functional food or therapeutic applications.</p><p><strong>Results: </strong>In this study, a galacturonic acid-rich heteropolysaccharide (UEDAP-4) was successfully extracted and isolated from dried apricots from Xinjiang, China. The polysaccharide is primarily composed of five monosaccharides: arabinose (Ara), rhamnose (Rha), galactose (Gal), glucose (Glc), and galacturonic acid (GalA), with a molar ratio of 28.78:20.36:6.87:5.51:38.48. The main chain of UEDAP-4 is characterized by the following structure:⟶4)-α-D-GalpA-(1 ⟶ 5)-α-L-Araf-(1 ⟶ 4)-α-D-GalpA-(1 ⟶ 2)-α-L-Araf-(1 ⟶ 3)-α-L-Araf-(1 ⟶ 4)-α-D-GalpA-(1 ⟶ 2)-α-L-Rhap-(1 ⟶ 4)-α-L-Rhap-(1 ⟶ 4)-α-D-GalpA-(1⟶, with three additional branched chains. The half-maximal inhibitory concentrations (IC₅₀) of UEDAP-4 against DPPH·, ·OH, and ABTS·⁺ were determined to be 5.399, 8.984, and 14.151 mg/mL, respectively. These results demonstrate that UEDAP-4 exhibits potent free radical scavenging activity, likely due to its high content of GalA and Ara.</p><p><strong>Conclusions: </strong>This study significantly advances our understanding of the relationship between the structural properties of polysaccharides derived from Xinjiang dried apricots and their antioxidant activity. This discovery highlights UEDAP-4 as a promising candidate for natural antioxidants, with potential applications in food preservation, pharmaceutical formulations, and functional food development.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"2526753"},"PeriodicalIF":2.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149017024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the Structural Variability of Doxycycline Hyclate: Mechanistic Insights and Comparative Assessment of Four Chemically Diverse Spectrophotometric Pathways.","authors":"Mona A Abdel Rahman, Reem H Obaydo","doi":"10.1155/jamc/2780011","DOIUrl":"10.1155/jamc/2780011","url":null,"abstract":"<p><p>Four distinct, chemically asynchronous spectrophotometric pathways each tapping into a different reactive node of the polyfunctional doxycycline hyclate (DOX) framework were planned to bypass the limitations of single mechanism assays. It works amazingly well. By exploiting the drug's inherent structural mutability, we developed a multipronged quantification matrix. Take the drug's reducing capacity, for instance. In Method A, this specific driving force reduces Fe (III) in the presence of potassium ferricyanide, yielding a highly conjugated Turnbull's blue complex characterized by a distinct bathochromic shift (<i>λ</i> _max 783.5 nm). Method B switches components entirely. It forces an alkaline electrophilic coupling with Fast Red B salt to lock down a resilient red azo chromophore (<i>λ</i> _max 498 nm). Method C determines for selective oxidation using N-bromosuccinimide (NBS) in a strongly acidic environment to generate a stable yellow derivative (<i>λ</i> _max 383.5 nm) (a sequence highly dependent on strict kinetic control). Method D abandons covalent modification altogether, relying instead on the generation of a lipophilic ion-pair associate with bromothymol blue (BTB) at pH 3.0, which partition readily into chloroform (<i>λ</i> _max 409.5 nm). Linearity spans diverse concentration intervals: 0.5-4, 5-60, 20-140, and 5-35 μg mL^-1 for protocols A through D. Sensitivity peaks at a remarkably low LOD of 0.16 μg mL^-1. Rigorous compliance with ICH validation protocols confirmed tight fidelity regarding accuracy and reproducibility. When applied to real-world commercial capsules, the matrix remained entirely unaffected by matrix excipients. This makes it an incredibly cheap, solvent-lean alternative for high-throughput benchtop quality control.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"2780011"},"PeriodicalIF":2.1,"publicationDate":"2026-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13526241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148864743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of an UPLC-MS/MS Method for Remodelin Quantification in Mouse Plasma and Tissues: Application to Biodistribution and Ultrastructural Assessment.","authors":"Litao Wang, Quanyu Liu, Cheng Yu, Yu Zhong, Minxia Wu, Xi Lin, Yan Hu","doi":"10.1155/jamc/9654340","DOIUrl":"10.1155/jamc/9654340","url":null,"abstract":"<p><p>Remodelin, a selective N-acetyltransferase 10 (NAT10) inhibitor, has emerged as a promising therapeutic candidate for degenerative diseases with nuclear abnormalities and acetylation dysregulation. However, its pharmacological development has been hindered by the lack of a sufficiently sensitive bioanalytical method. To address this limitation, a simple, sensitive, and reliable ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method was established and fully validated. This method achieved accurate quantification of remodelin at low pg/mL levels, representing a significant improvement in sensitivity and excellent linearity (<i>R</i> ² > 0.99). All validation parameters met the predefined bioanalytical acceptance criteria. This method was applied to quantify remodelin in C57BL/6J mouse plasma and various tissues (carotid artery, heart, liver, and kidney), with higher concentrations observed in the liver and kidney. Given the proposed role of remodelin in maintaining nuclear integrity, its protective effect was further investigated by transmission electron microscopy (TEM). The analysis provided direct evidence of a protective effect on nuclear morphology, with treated tissues maintaining intact nuclear membranes and clear cellular architecture, whereas untreated tissues exhibited varying degrees of structural damage. These findings established a robust analytical method for remodelin quantification and provided preliminary ultrastructural evidence of its protective effects, supporting further pharmacodynamic investigation.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9654340"},"PeriodicalIF":2.1,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148812822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC-Triple Quadrupole MS Enables Sample Classification in <i>Viscum combreticola</i>: Detecting Subtle Photochemical Isomerization in Hydroxycinnamic Acid Derivatives.","authors":"Mailane Rose Sekgobela, Anza-Tshilidzi Ramabulana, Lutendo Michael Mathomu, Babra Moyo, Ntakadzeni Edwin Madala","doi":"10.1155/jamc/7032335","DOIUrl":"10.1155/jamc/7032335","url":null,"abstract":"<p><p>High-resolution MS techniques such as time-of-flight (TOF)-MS are preferred for putative metabolite identification due to their unprecedented high resolution and accuracy in metabolomics studies. However, exploring cost-effective alternative instruments for routine analyses would be advantageous, particularly in laboratories where access to high-resolution mass spectrometry (MS) is limited. This study investigated the ability of the LC-QQQ-MS instrument operating in Q3 scan mode in replicating results obtained from the qTOF-MS. Methanolic extracts of non-treated and UV-treated samples of <i>Viscum combreticola</i> were used as model samples for the analysis. Multivariate statistical models including principal component analysis (PCA), hierarchical clustering analysis and volcano plots were employed to highlight metabolite differences between UV-treated and non-treated plant extracts. The findings of this study demonstrated that the LC-QQQ-MS is effective in distinguishing between the two contrasting extracts of <i>V. combreticola</i>. Several <i>cis</i> isomers of hydroxycinnamic acid containing molecules such as 3-feruloylquinic acid, 4,5-dicaffeoylquinic acid and 4-<i>p</i>-coumaroyl-5-caffeoylquinic acids that underwent geometrical isomerization following UV exposure were identified as differentiating molecules (biomarkers), consistent with previous findings using high-resolution qTOF-MS instruments. This study positions QQQ-MS as a valuable tool for authenticating samples containing known metabolites, particularly when the identification of unknowns is not the primary objective and high-resolution MS instruments are not accessible.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"7032335"},"PeriodicalIF":2.1,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Plastic Composition in the Combustion Material on the Persistent Organic Pollutant Content in Smoked Dried Shiitake Mushroom.","authors":"Uyen Nguyen-Thu, Giang Do-Hoang, Luyen Nguyen-Thi, Minh Nguyen-Thi-Thu, Duong Hoang-Thuy, Le Bui-Thi-Nhat, Ngoan Nguyen-Thi, Anh Hoang-Le-Tuan, Tung Nguyen-Ngoc, Dat Nguyen-Tien","doi":"10.1155/jamc/1257545","DOIUrl":"10.1155/jamc/1257545","url":null,"abstract":"<p><p>Smoking is a traditional preservation method in Vietnam, widely applied to shiitake mushrooms (<i>Lentinula edodes</i>) as well as meats, yet the use of plastic-contaminated fuels poses emerging food safety concerns. This study provides the first congener-specific characterization of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and polychlorinated dibenzo-<i>p</i>-dioxins and furans (PCDD/Fs) in smoke-dried shiitake produced with wood and wood adulterated by polyethylene (PE), polystyrene (PS), or polyvinyl chloride (PVC). Using GC-MS/MS combined with multivariate analyses, we observed clear differences in contaminant accumulation driven by fuel composition. Clean wood (W) yielded the lowest burdens, with PCB levels < 150 ng/kg, PCDD/Fs negligible, and PAHs < 650 µg/kg. Plastic-adulterated fuels significantly increased contamination (Kruskal-Wallis <i>p</i> < 0.001), with PAH and PCB/PCDD/F levels following robust gradients of W < PE < PS < PVC (PCBs/PCDD/Fs) and W < PE < PVC < PS (PAHs). PE was characterized by light PAHs (naphthalene, acenaphthylene, and acenaphthene) and moderate PCB increases; PS, by high-molecular-weight PAHs and coplanar PCBs; and PVC, by the broadest enrichment, including nearly all tested PCDD/F congeners. Hierarchical cluster analysis and PCA confirmed clustering by fuel type and revealed pollutant fingerprints specific to each plastic. These findings demonstrate, for the first time, the toxicological risks of plastic-contaminated smoking fuels in Vietnamese shiitake processing and provide baseline data essential for risk assessment and food safety policy.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"1257545"},"PeriodicalIF":2.1,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13435889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Simple and Accurate LC-MS/MS Method for the Determination of Indobufen in Human Plasma and Its Clinical Application.","authors":"Hongyan Ji, Haijun Qu, Qie Guo, Donghua Liu, Changli Xu, Xue Yang, Fanbo Jin, Wen Xu","doi":"10.1155/jamc/2232924","DOIUrl":"10.1155/jamc/2232924","url":null,"abstract":"<p><p>As an antithrombotic agent, indobufen is widely used in China. However, the relationship between drug concentration and therapeutic efficacy in specific patient populations remains unclear. Therefore, it is crucial to establish a method for quantifying indobufen concentrations in human plasma to guide dosage recommendations. An electrospray ionization (ESI) power supply was utilized in conjunction with multireaction monitoring (MRM) in positive ion mode. Protein precipitation was employed to extract indobufen from the plasma sample. Separation was carried out using a ChromCORE-C18 column with a gradient elution of the mobile phase, which was composed of 0.1% formic acid in water (Solvent A) and methanol (Solvent B) at a flow rate of 0.3 mL/min. The typical standard curve equation was <i>Y</i> = 0.763<i>X</i> + 0.0986 (<i>r</i> = 0.9993), demonstrating a good linear relationship within the range of 0.5-30 μg/mL. All methodological outcomes complied with regulatory guidelines. In patients with moderate renal insufficiency, it is advisable to reduce the dosage to 100 mg once daily. For patients with severe renal insufficiency, the dosage should be reduced to 1/3-1/4 of the standard dose. For patients undergoing continuous renal replacement therapy (CRRT), indobufen can be gradually eliminated, and most patients do not require dose adjustment.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"2232924"},"PeriodicalIF":2.1,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13408705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148619827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}