Journal of Analytical Methods in Chemistry最新文献

{"title":"Breath by Breath: Navigating the Landscape of Exhaled Breath in Clinical Research and Prospects for the Future.","authors":"Nisha Parikh, Hetansh Rao, Akshada Dabhade, Vishwa Patel, Vikalp Shroff, Deep Prajapati, Palak Parikh, Ketan Ranch, B Mahalakshmi, Shery Jacob, Sai H S Boddu","doi":"10.1155/jamc/9959007","DOIUrl":"https://doi.org/10.1155/jamc/9959007","url":null,"abstract":"<p><p>Exhaled breath analysis (EBA) has evolved as a noninvasive diagnostic technique with enormous promise in healthcare. The development in this area is based on the concept that the breathalyzers employed to measure the alcohol concentration in the blood can be utilized to evaluate health by analyzing the intricate chemical makeup of exhaled breath, which includes various volatile organic compounds (VOCs). Breath analysis could be the driver of personalized medicine and used in early disease identification as it involves the detection of a variety of disease-specific biomarkers. In diagnostics, EBA offers a noninvasive procedure of illness identification and monitoring. This approach allows for early detection, personalized treatment options, and therapeutic monitoring by detecting disease-specific VOC biomarkers such as acetone in diabetes and ethane in lipid peroxidation. Also, EBA can measure environmental exposures such as air pollution or occupational dangers. By identifying VOCs from various sources, researchers can measure exposure levels and assess related health hazards. Advances in the analytical methods, including mass spectrometry, gas chromatography, and sensor arrays made it possible to quantify and identify VOCs in exhaled air with high sensitivity and precision. This review focuses on the biomarkers present in breath and their role in identifying diseases. It covers the essential compounds found in breath and their biochemical processes. Furthermore, an in-depth examination of different methods for identifying biomarkers in breath and the factors influencing VOC concentrations, including age, storage conditions, external sources, and the difference between dead space air and alveolar air, has been discussed.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9959007"},"PeriodicalIF":2.2,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13147357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Free Testosterone Chemiluminescence Detection Kit and Its Clinical Application.","authors":"Shuang Han, Min Liu, Simin Zhao, Wenjun Yan, Zhixian Lu, Xuan Huang","doi":"10.1155/jamc/5165899","DOIUrl":"https://doi.org/10.1155/jamc/5165899","url":null,"abstract":"<p><strong>Background: </strong>Free testosterone (FT), the bioactive form comprising 1%-2% of total testosterone, directly enters cells. Unlike total testosterone, FT levels are less affected by sex hormone-binding globulin and better reflect biological activity. Accurate serum FT measurement is crucial for diagnosing conditions like male hypogonadism, PCOS, metabolic syndrome, osteoporosis, and Alzheimer's. However, existing methods suffer from inadequate sensitivity, complexity, and high cost, necessitating improved detection technologies. We developed and evaluated a chemiluminescence assay kit for FT.</p><p><strong>Methods: </strong>FT was quantified using a competitive immunoassay where sample FT competes with acridinium ester-labeled testosterone derivatives for binding to biotinylated antitestosterone antibodies on magnetic beads. Key parameters (magnetic bead concentration, biotinylation, labeling, and antibody/derivative concentrations) were optimized. Kit performance was rigorously assessed for linearity, limit of blank (LoB), accuracy, precision, stability, specificity, and clinical relevance. FT levels were measured in 1615 male and 2035 female patient samples to analyze clinical significance.</p><p><strong>Results: </strong>The assay demonstrated excellent linearity (<i>r</i> > 0.99), low LoB (0.021 pg/mL), high accuracy (deviation < 5%), precision (CV < 5%), and 12-month stability. Specificity testing showed no cross-reactivity. Method comparison with 392 clinical samples yielded a strong correlation (<i>r</i> = 0.9941). Analysis of patient samples revealed significant FT level differences among males with various diagnoses: lower levels in prostate cancer patients and higher levels in conditions like hair loss.</p><p><strong>Conclusion: </strong>The developed chemiluminescence FT assay kit exhibits superior performance, low cost, and high automation, fully meeting clinical requirements. FT measurement provides a valuable reference for diagnosing and assessing specific diseases, aiding improved clinical management.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"5165899"},"PeriodicalIF":2.2,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147772080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of Potential Antidepressant Active Ingredients of <i>Albiziae Flos</i> via HS-GC-MS, Chemometrics, and Network Pharmacology.","authors":"Dan Yang, Dan He, Yiwu Wang, Yuan Shen, Jialing Yu, Ruijia Yang, Lin Yang","doi":"10.1155/jamc/2885034","DOIUrl":"https://doi.org/10.1155/jamc/2885034","url":null,"abstract":"<p><p><i>Albiziae Flos</i> (AF) is a traditional Chinese medicine with an extensive historical background. This study presented an integrated approach combining headspace-gas chromatography-mass spectrometry (HS-GC-MS), chemometrics, and network pharmacology to comprehensively evaluate the volatile components of AF and explore their potential antidepressant mechanisms. A total of 34 volatile compounds were identified through HS-GC-MS analysis. Fingerprint assessment revealed high consistency among 16 batches (similarity: 0.790-0.998), while chemometric analysis successfully discriminated samples from different geographical origins. Network pharmacology screening identified 15 active components and 131 potential targets, revealing multicomponent, multitarget characteristics of AF's antidepressant effects. Molecular docking simulations demonstrated strong binding affinity between linalool oxide configurations and 12 core targets, primarily through hydrogen bonding and hydrophobic interactions. This work lies in its comprehensive investigation of AF's volatile components using an integrated analytical-pharmacological approach, providing both a methodological framework for quality assessment and mechanistic insights for antidepressant drug development. Our findings established scientific foundations for quality control of AF and revealed its potential antidepressant mechanisms through multiple pathways.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"2885034"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12950218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Colorimetric Multimetabolite Assay for Quantitative Measurement of Keto Acids in Urine for At-Home Monitoring of Metabolic Disorders.","authors":"Dipanjan Bhattacharyya, Abby Kropielnicki, Brian L Lee, Yeganeh Khaniani, Marcia A LeVatte, David S Wishart","doi":"10.1155/jamc/4116313","DOIUrl":"https://doi.org/10.1155/jamc/4116313","url":null,"abstract":"<p><p>Inborn errors of metabolism such as phenylketonuria (PKU) and maple syrup urine disease (MSUD) can cause severe developmental problems. Both conditions can lead to harmful levels of keto acids in biofluids-phenylpyruvic acid (PPA) in PKU and branched-chain α-keto acids in MSUD. Monitoring urinary keto acids helps track dietary adherence and reduces the risk of metabolic crisis. However, current at-home tests are qualitative and difficult to interpret, while existing metabolomic assays require expensive equipment and must be conducted in a lab. This study aimed to develop a simple, quantitative, rapid, at-home assay for detecting multiple urinary keto acids associated with PKU and MSUD. A modified two-step 2,4-dinitrophenylhydrazine (DNPH)-based multimetabolite assay was developed, where sodium hydroxide (NaOH) converts the yellow hydrazone precipitate to a stable amber solution, enabling quantification of multiple keto acids (700-7200 μM) within 10 min. The assay was validated using spiked urine samples and adapted into a prototype at-home kit using caprolactam-immobilized NaOH. Nuclear magnetic resonance (NMR)-based metabolomics was used as a reference method to authenticate readings from a PKU patient. Correlation studies demonstrated strong linearity for MSUD (<i>R</i> ² = 0.91-0.96)- and PKU (<i>R</i> ² = 0.95-0.99)-spiked samples. Quantification of keto acids in authentic PKU urine samples showed excellent agreement with the results of quantitative NMR-based metabolomics assays (<i>R</i> ² = 0.99). Low-cost, at-home colorimetric tests for urinary keto acids could enable screening, detection, and monitoring of PKU and MSUD in the 90% of the world without access to advanced metabolic clinics.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"4116313"},"PeriodicalIF":2.2,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12949077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147326034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Hemolysis on Biochemical Tests: Determining Interference Cutoffs and Managing Hemolytic Samples.","authors":"Babak Shirazi Yeganeh, Neda Soleimani, Saeedeh Zare, Sahand Mohammadzadeh, Farzaneh Amiri, Mohammad Javad Esmaeili, Davoud Soleimani, Soodabeh Khoshnyat","doi":"10.1155/jamc/9307894","DOIUrl":"https://doi.org/10.1155/jamc/9307894","url":null,"abstract":"<p><strong>Background: </strong>Hemolysis is a common source of interference in biochemical tests, potentially leading to significant errors in clinical decision-making and patient management. This study aimed to evaluate the impact of varying degrees of hemolysis on routine biochemical analytes and to find a local hemolytic index (HIX) cutoff for safe reporting.</p><p><strong>Methods: </strong>This experimental study was conducted on 30 serum samples from healthy individuals with normal biochemical profiles. Baseline concentrations of 20 routine analytes were measured using a DIRUI CS-1200 analyzer. To simulate hemolysis, increasing concentrations of autologous hemolysate were added to each sample, creating three grades of hemolysis based on HIX (mild, moderate, and severe). All analytes were remeasured, and the absolute and relative biases were calculated against baseline levels. These biases were then compared with the CLIA-defined total allowable error (TEa) to obtain the safe reporting cutoff for each analyte.</p><p><strong>Results: </strong>All analytes showed a positive bias with increasing hemolysis, though the magnitude of interference varied significantly. LDH, uric acid, AST, and total bilirubin, in that order, were more impacted by hemolysis. In mild hemolysis (HIX < 0.5), none of the analyte biases exceeded TEa limits (<i>p</i> value < 0.05). In moderate and severe hemolysis, however, most exceeded acceptable limits. Based on these results, a HIX cutoff of 0.5 was regarded for reporting mildly hemolyzed samples.</p><p><strong>Conclusion: </strong>Hemolysis interference is both analyte- and system-dependent. Laboratories are recommended to conduct similar research tailored to their local laboratory settings and population to improve analytical quality and reduce unnecessary specimen rejection.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9307894"},"PeriodicalIF":2.2,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12946476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147326036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and Mechanism Prediction of Active Components in Fuganlin Oral Liquid for Respiratory Tract Infections Using UPLC-Q-TOF-MS and Network Pharmacology.","authors":"Mengyue Zhang, Feng Han, Mingxuan Yang, Zhishan Ye, Ying Cui, Yuefei Wang, Jing Yang, Xin Chai","doi":"10.1155/jamc/3075224","DOIUrl":"10.1155/jamc/3075224","url":null,"abstract":"<p><p>Fuganlin oral liquid (FOL) has been clinically employed for the treatment of pediatric qi deficiency colds, manifesting symptoms such as fever, cough, asthma, and sore throat. However, the chemical composition and bioactive components of FOL have not been clearly elucidated. In this study, a comprehensive qualitative analysis of FOL was conducted utilizing ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry in conjunction with network pharmacology. A total of 124 chemical components were tentatively characterized, comprising flavonoids, phenolic acids, saponins, coumarins, and others. Among these, 43 compounds were unequivocally identified by comparison with authentic reference standards. Furthermore, network pharmacology analysis revealed that the ingredients of FOL exhibited anti-inflammatory properties and demonstrated potential efficacy in relieving cough and asthma associated with respiratory tract infections. Collectively, this study provides the first comprehensive characterization of the chemical composition in FOL and explores the potential pharmacological mechanisms of its bioactive constituents, thereby providing scientific support for quality control standards and clinical applications.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"3075224"},"PeriodicalIF":2.2,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12894422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Making of the Vindolanda Wooden Writing Tablets: A Noninvasive Multianalytical Protocol for the Characterisation of Black Roman Inks.","authors":"Giovanna Vasco, Joanne Dyer, Richard Hobbs, Caroline R Cartwright, Alessandro Buccolieri","doi":"10.1155/jamc/5142007","DOIUrl":"10.1155/jamc/5142007","url":null,"abstract":"<p><p>Within the 'Making History' project, the British Museum investigated the materiality of the Vindolanda ink writing tablets for the first time, with a particular focus on the possible differentiation of the ink sources employed. Thanks to the application of complementary scientific techniques, it was possible to develop an analytical protocol for the documentation of the ink writing from the palaeographic and conservation points of view and the characterisation of their manufacture. From the macroscopic to the microscopic scale, the use of imaging techniques (MBI, SWIR and digital microscope) highlighted the unique features of the different handwriting styles and allowed the identification of the ink typology. Raman spectroscopy and elemental analyses (XRF and SEM-EDX) were then applied to gain information about the chemical-physical nature of the inks. The main achievement was the possibility to differentiate the sources of carbon-based inks using Raman spectroscopy and multivariate analysis. It was possible to examine ink production at the edge of the Roman Empire, comparing it with the ancient literature and the different practices arising in the Mediterranean area.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"5142007"},"PeriodicalIF":2.2,"publicationDate":"2026-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12884006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146157309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive and Rapid Chemical Profiling of <i>Cirsium japonicum</i> DC Utilizing UHPLC-Q-Orbitrap MS With Parallel Reaction Monitoring.","authors":"Jia-Yi Wang, Yu-Feng Zou, Kai-Lin Li, Yi-Fan Chen, Ling Liu, Shu-Sen Wu, Hui Li","doi":"10.1155/jamc/5782099","DOIUrl":"10.1155/jamc/5782099","url":null,"abstract":"<p><p><i>Cirsium japonicum</i>, a traditional medicinal plant, has been widely used for its therapeutic properties in treating various ailments. However, a comprehensive analysis of its chemical composition remains limited, hindering a full understanding of its chemical basis and pharmacological activities. This study aims to identify and characterize the chemical constituents of <i>C. japonicum</i> using ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap MS) and parallel reaction monitoring (PRM). A total of 94 compounds were identified, including 57 organic acids, 25 flavonoids, 3 phenylpropanoids, and 9 other components, with chlorogenic acid dominating the organic acid fraction. Organic acids and caffeic acid, among organic acid compounds, exhibit hemostatic effects based on prior evidence. The UHPLC-Q-Exactive Orbitrap MS-based approach provided a detailed chemical profile of <i>C. japonicum</i>, which could facilitate a deeper understanding of its medicinal properties and guide future pharmacological studies. The identified compounds can be used as potential biomarkers for the standardization and quality control of medicinal products of <i>C. japonicum</i>.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"5782099"},"PeriodicalIF":2.2,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12859162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Detection of Organochlorine Pesticide Residues in Sesame Seeds (<i>Sesamum indicum L</i>.) Using Advanced GC-MS/MS Techniques.","authors":"Kero Assefa Ago, Molla Tefera","doi":"10.1155/jamc/8312847","DOIUrl":"10.1155/jamc/8312847","url":null,"abstract":"<p><p>Considering the economic and edible values of sesame seeds, it is important to monitor sesame seed for the safety of consumers and for international trade as it helps the country government and suppliers as products to gain market acceptance more effectively. The objective of this study was to determine selected organochlorine pesticide (OCP) residues in sesame seeds. Quick, easy, cheap, efficient, rugged, and safe method followed by gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) was used for the rapid separation and determination of 20 OCPs in sesame seeds. Acetonitrile in combination with 1% glacial acetic acid was used as an extraction solvent. Primary secondary amine, graphitized carbon black, and octadecylsilane in QuEChERS kit were used for d-SPE clean up before GC-MS/MS analysis. The GC-MS/MS analysis was evaluated in terms of linearity, recovery, and precision. The calibration curves were obtained for all analytes and displayed good linearity over the selected concentration range with regression coefficients (<i>r</i> ²) ≥ 0.999. The recoveries for spiked analytes in sesame seed samples were ranged from 93.58 to 115.81 with RSDs lower than 1%. The LOD and LOQ for all investigated pesticides were in the range of 0.05-0.88 μg/kg and from 0.16 to 2.93 μg/kg, respectively.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"8312847"},"PeriodicalIF":2.2,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12817196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Hollow Fiber-Supported ZIF-8@GO Reinforced Sol-Gel for Preconcentration of Paraquat Before Determination by UV-Vis Spectrophotometry\".","authors":"","doi":"10.1155/jamc/9874058","DOIUrl":"10.1155/jamc/9874058","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/jamc/6883692.].</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9874058"},"PeriodicalIF":2.2,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12812251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}