Journal of Analytical Methods in Chemistry最新文献

Hollow Fiber-Supported ZIF-8@GO Reinforced Sol-Gel for Preconcentration of Paraquat Before Determination by UV-Vis Spectrophotometry. 中空纤维支撑ZIF-8@GO增强溶胶-凝胶在紫外可见分光光度法测定前的预富集。

IF 2.3 3区化学

Journal of Analytical Methods in Chemistry Pub Date : 2025-06-21 eCollection Date: 2025-01-01 DOI: 10.1155/jamc/6883692

Narges Vaezi, Naser Dalali

{"title":"Hollow Fiber-Supported ZIF-8@GO Reinforced Sol-Gel for Preconcentration of Paraquat Before Determination by UV-Vis Spectrophotometry.","authors":"Narges Vaezi, Naser Dalali","doi":"10.1155/jamc/6883692","DOIUrl":"10.1155/jamc/6883692","url":null,"abstract":"A novel, sensitive, and efficient method was developed for the determination of paraquat in soil samples, centered on solid-phase microextraction using hollow fiber-supported ZIF-8@GO reinforced sol-gel combined with UV-Vis spectrophotometry at 257 nm. Silica-based ZIF-8@GO was synthesized through sol-gel technology by reacting tetraethyl orthosilicate (TEOS) with hydrochloric acid (HCl) as a catalyst. The resulting solution was injected into hollow polypropylene fiber segments for in situ gelation. Key microextraction parameters, such as the donor phase pH and volume, stirring rate, extraction time, and desorption conditions (solvent type and volume), were systematically studied and optimized. Under optimal conditions, the method demonstrated linearity within the range of 0.5-2000 μg L-1, with a correlation coefficient of 0.999. The relative standard deviation for seven replicate measurements at a concentration of 600 μg L-1 paraquat was 0.4%. A preconcentration factor of 631 and a detection limit of 0.15 μg L-1 were achieved. Validation of the method was conducted by analyzing soil samples from various sites, yielding recoveries between 96% and 105% with low relative standard deviations, indicating its high accuracy and reliability in detecting trace levels of paraquat.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"6883692"},"PeriodicalIF":2.3,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Facile Method for Screening DPP IV Inhibitors in Living Cell System Based on Enzyme Activity Probe. 基于酶活性探针筛选活细胞系统中DPP IV抑制剂的简便方法

IF 2.3 3区化学

Journal of Analytical Methods in Chemistry Pub Date : 2025-06-16 eCollection Date: 2025-01-01 DOI: 10.1155/jamc/1616740

Shu-Mei Pan, Chun-Yu Xing, Hong-Wei Li, Rui-Min Wang, Xin-Yue Pu, Tie-Gang Wang, Dan-Dan Wang, Li-Wei Zou

{"title":"A Facile Method for Screening DPP IV Inhibitors in Living Cell System Based on Enzyme Activity Probe.","authors":"Shu-Mei Pan, Chun-Yu Xing, Hong-Wei Li, Rui-Min Wang, Xin-Yue Pu, Tie-Gang Wang, Dan-Dan Wang, Li-Wei Zou","doi":"10.1155/jamc/1616740","DOIUrl":"10.1155/jamc/1616740","url":null,"abstract":"Dipeptidyl peptidase IV (DPP IV), one of the most essential peptidase, is widely distributed in various organs and tissues of the human body, which affects protein stability by acting on peptide bonds at the end of peptide chains and is further involved in physiological processes such as cellular metabolism and signaling. Recent studies have shown that DPP IV is not only involved in normal physiological processes but also closely related to various pathological processes, making it an important target for the treatment of metabolic diseases. Deciphering the relevance of DPP IV to human diseases and screening the inhibitors of DPP IV requires reliable tools, which can sense the function of this key enzyme in complex biological samples. Therefore, we aimed to construct a simple and easy-to-use assay for human DPP IV activity in a living cell system to achieve highly sensitive and selective detection of DPP IV function and further screening for its inhibitors. An easy-to-use assay for DPP IV function at the cellular level with a specific fluorescence probe toward DPP IV using a fluorescence microplate reader was the first to be established. Then, the experimental conditions were systematically optimized in terms of cell density, concentrations of probe, and incubation time. After that, the efficacy of the positive DPP IV inhibitors was evaluated by utilizing the optimized easy-to-use assay. Overall, this easy-to-use assay exhibited good precision, robustness, high throughput, and reliability. In conclusion, a straightforward and user-friendly method has been developed for detecting DPP IV activity in live cells, enabling accurate assessment of DPP IV function and efficient screening of inhibitors. This method may hold significant implications for advancing understanding of the relationship between DPP IV and disease progression, early disease diagnosis, and personalized drug therapy.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"1616740"},"PeriodicalIF":2.3,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Competitive ELISAs Suitable for Detection of Recombinant Human Growth Hormone in Illegal Nutritional Supplements. 适用于非法营养补充剂中重组人生长激素检测的竞争性elisa试剂盒的建立。

IF 2.3 3区化学

Journal of Analytical Methods in Chemistry Pub Date : 2025-06-06 eCollection Date: 2025-01-01 DOI: 10.1155/jamc/3021570

L Karamonová, B Procházková, M Volný, A Nemeškalová, D Sýkora, M Kuchař, L Fukal, B Holubová

{"title":"Development of Competitive ELISAs Suitable for Detection of Recombinant Human Growth Hormone in Illegal Nutritional Supplements.","authors":"L Karamonová, B Procházková, M Volný, A Nemeškalová, D Sýkora, M Kuchař, L Fukal, B Holubová","doi":"10.1155/jamc/3021570","DOIUrl":"10.1155/jamc/3021570","url":null,"abstract":"The use of human growth hormone (hGH) by athletes remains widespread despite being banned by the World Anti-Doping Agency (WADA). Current commercial ELISA kits for the determination of hGH are predominantly based on the sandwich format of this method, which allows the analysis of the whole hGH molecules, but not their fragments. The abuse of these fragments by athletes is the current trend in doping. Therefore, a competitive ELISA format is needed; however, only a limited number of kits on the market are based on this principle. Furthermore, these kits are designed to detect and quantify hGH only in blood plasma, serum or tissue cultures. When analysing a nutritional supplement containing hGH using one of these ELISA kits, a false negative result was observed. In the present work, two competitive ELISA methods for the detection of hGH in illegal nutritional supplements were developed. The development of the methods involved the selection of suitable immunoreagents and their combinations with subsequent optimisation of the conditions for their use. The methods were then characterised (with LOD 0.4 and 18.4 ng/mL, according to the primary antibody) and applied to analyse real nutritional supplements. In all 34 samples suspected of containing hGH, the hormone was detected using both ELISA methods and subsequently confirmed by LC-UV and LC-MS. Therefore, the newly developed ELISA methods could become a valuable tool for the police in identifying illegal preparations.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"3021570"},"PeriodicalIF":2.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Safflower Adulteration in Saffron Using Ion Mobility Spectroscopy. 离子迁移光谱法检测藏红花中红花的掺假。

IF 2.3 3区化学

Journal of Analytical Methods in Chemistry Pub Date : 2025-05-27 eCollection Date: 2025-01-01 DOI: 10.1155/jamc/6366923

Mahtab Heyrani, Mohammad-Taghi Golmakani, Mohammadreza Khalesi

{"title":"Detection of Safflower Adulteration in Saffron Using Ion Mobility Spectroscopy.","authors":"Mahtab Heyrani, Mohammad-Taghi Golmakani, Mohammadreza Khalesi","doi":"10.1155/jamc/6366923","DOIUrl":"10.1155/jamc/6366923","url":null,"abstract":"Saffron (Crocus sativus L.), an exceptionally valuable and expensive spice on an international scale, has become the target of a rapid increase in fraudulent practices. In an effort to decrease expenses, stigmas of safflower (Carthamus tinctorius), which closely resemble saffron, are often added to pure saffron as a typical method of adulteration. Hence, by quantifying the extent of eugenol modifications in the samples and employing ion mobility spectrometry (IMS) to identify and quantify these adulterants in saffron, the objective of this research has been accomplished. The analysis of eugenol showed a significant increase in peak intensity as the concentration of safflower increased in laboratory-prepared samples of pure saffron and safflower as well as the mixture of them (25%, 50%, 75%, and 100%, v/v). In the subsequent phase, a total of 20 saffron samples procured from nearby markets were examined under an optical microscope to identify any adulteration with safflower. Five samples, which included saffron containing safflower at varying concentrations (8.3%, 14.9%, 19.4%, 25.4%, and 33.7% W/W), were chosen for additional IMS analysis. The results showed that the peak intensity of eugenol climbed from 0.20 to 0.28 mV by augmenting the safflower content in saffron. Therefore, by increasing the level of safflower contamination in saffron, the concentration of eugenol in the IMS rose. The outcomes demonstrated that the selection method effectively detects saffron adulterated with safflower, improving both precision and specificity, and could aid in defining standard quality control procedures for saffron authenticity and quality.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"6366923"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12133364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Active Ingredients and Potential Mechanism of Qijia Rougan Decoction in Autophagy and Hepatic Stellate Cell Activation Modulation in Liver Fibrogenesis. 七家柔肝汤在肝纤维化自噬和肝星状细胞活化调节中的有效成分及其潜在机制。

IF 2.3 3区化学

Journal of Analytical Methods in Chemistry Pub Date : 2025-05-12 eCollection Date: 2025-01-01 DOI: 10.1155/jamc/4646858

Gui-Yu Li, Bai-Xue Li, Hong-Fei Song, Jie-Wen Gou, Li Wen, Quan-Sheng Feng

{"title":"The Active Ingredients and Potential Mechanism of Qijia Rougan Decoction in Autophagy and Hepatic Stellate Cell Activation Modulation in Liver Fibrogenesis.","authors":"Gui-Yu Li, Bai-Xue Li, Hong-Fei Song, Jie-Wen Gou, Li Wen, Quan-Sheng Feng","doi":"10.1155/jamc/4646858","DOIUrl":"10.1155/jamc/4646858","url":null,"abstract":"Background and Objectives: Liver fibrosis results from chronic inflammation. Qijia Rougan decoction, a traditional Chinese medicinal formulation, shows hepatoprotective potential, yet its mechanisms remain unclear. This study aims to investigate its antifibrotic effects and underlying mechanisms. Methods: Rat liver fibrosis was induced by carbon tetrachloride (CCl4) and ethanol exposure. Histopathological assessment was performed using hematoxylin-eosin (HE) and Masson's trichrome staining. Hepatic stellate cell (HSC) activation and autophagic processes were examined through western blot analysis, immunofluorescence staining, and other in vitro assays. Components of Qijia Rougan decoction were analyzed by BATMAN-TCM platform. The pharmacological network was constructed using BATMAN-TCM platform, while disease-related targets were identified through DisGeNET database. Pathway enrichment analysis was conducted using KEGG pathway database. Results: Significant reductions in hepatic index and serum biomarkers (ALT, AST, ALP, TBA, and γ-GT) were observed following Qijia Rougan decoction treatment, with maximal efficacy at 6 weeks. The decoction downregulated of LC3B and α-SMA expression in fibrotic tissues. In vitro, it suppressed LPS-induced α-SMA expression and autophagosome formation in HSC-T6 cells. Network pharmacology analysis of Qijia Rougan decoction identified 274 bioactive compounds and 12,883 potential targets, with pathway analysis indicating PI3K/AKT signaling as the predominant regulatory mechanism. Conclusion: Qijia Rougan decoction alleviates liver fibrosis, potentially by inhibiting HSC activation and autophagy processes via PI3K/AKT/mTOR pathway.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"4646858"},"PeriodicalIF":2.3,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12088832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the Extraction Conditions and Evaluation of Bioactivities of the Phenolic Enrichment From Pandanus amaryllifolius Leaves. 香豆叶酚类富集物提取条件优化及生物活性评价。

IF 2.3 3区化学

Journal of Analytical Methods in Chemistry Pub Date : 2025-05-07 eCollection Date: 2025-01-01 DOI: 10.1155/jamc/5256388

Do Hoang Giang, Bui Thi Nhat Le, Nguyen Thi Thu Minh, Nguyen Thi Thu Thuy, Nguyen Hai Dang, Hoang Le Tuan Anh, Nguyen Ngoc Tung, Nguyen Tien Dat

{"title":"Optimization of the Extraction Conditions and Evaluation of Bioactivities of the Phenolic Enrichment From Pandanus amaryllifolius Leaves.","authors":"Do Hoang Giang, Bui Thi Nhat Le, Nguyen Thi Thu Minh, Nguyen Thi Thu Thuy, Nguyen Hai Dang, Hoang Le Tuan Anh, Nguyen Ngoc Tung, Nguyen Tien Dat","doi":"10.1155/jamc/5256388","DOIUrl":"https://doi.org/10.1155/jamc/5256388","url":null,"abstract":"This study investigates the optimal conditions to enrich the phenolic content of the extract from Pandanus amaryllifolius leaves and evaluates the bioactivities of this enrichment. The phenolic enrichment was prepared under optimized conditions using the response surface methodology (RSM) with the Box-Behnken design. The antioxidant properties were assessed using DPPH and hydroxyl radical scavenging assays, while the NO production inhibition was measured in LPS-stimulated RAW 264.7 macrophage cells. Results indicated that the phenolic enrichment showed potent antioxidant activity comparable to ascorbic acid and catechin and significantly higher NO inhibition than the separated nonalkaloid and alkaloid fractions. The study also highlights the synergistic effect of phenolic and alkaloid compounds on the antioxidants and anti-inflammatory activities of the phenolic enrichment from Pandanus amaryllifolius leaves.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"5256388"},"PeriodicalIF":2.3,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the Active Ingredients and Prediction of the Potential Anticolitis Mechanism of the Feng-Liao-Chang-Wei-Kang Capsule via Mass Spectrometry and Network Pharmacology. 风辽长胃康胶囊有效成分表征及抗结肠炎机制的质谱及网络药理学预测。

IF 2.3 3区化学

Journal of Analytical Methods in Chemistry Pub Date : 2025-05-05 eCollection Date: 2025-01-01 DOI: 10.1155/jamc/2948965

Tingting Liu, Zhijiang He, Witiao Lv, Liyun Deng, Xizhe Sun, Yanfei Chen

{"title":"Characterization of the Active Ingredients and Prediction of the Potential Anticolitis Mechanism of the Feng-Liao-Chang-Wei-Kang Capsule via Mass Spectrometry and Network Pharmacology.","authors":"Tingting Liu, Zhijiang He, Witiao Lv, Liyun Deng, Xizhe Sun, Yanfei Chen","doi":"10.1155/jamc/2948965","DOIUrl":"10.1155/jamc/2948965","url":null,"abstract":"The Feng-Liao-Chang-Wei-Kang (FLCWK) capsule is a nationally protected Chinese patent medicine for the treatment of colitis. However, the potential active components and the pharmacological mechanism underlying the anticolitis effect of the FLCWK capsule remain unclear. This study aimed to reveal the active ingredients and possible anticolitis mechanism of the FLCWK capsule using an integrated approach combining mass spectrometry and network pharmacology analysis. Ultra-performance liquid chromatography plus Q-Exactive Orbitrap tandem mass spectrometry (UPLC-Q-Exactive Orbitrap MS) was applied to identify the components of the FLCWK capsule. A network pharmacology study, including target gene prediction and functional enrichment, was applied to screen the active ingredients of the FLCWK capsule and explore its potential mechanism for the treatment of colitis. A total of 115 components were identified in the FLCWK capsule. Network pharmacology results showed that 46 of these compounds with good bioavailability and drug-likeness, such as 4',5-dihydroxyflavone, pinostrobin, naringenin chalcone, apigenin, and morin, were selected as active ingredients. The active ingredients may act on 352 core protein targets, including EGFR, AKT1, PIK3R1, PIK3CB, and MAPK1, thereby modulating relevant pathways, such as MAPK and PI3K-Akt signaling pathways, and thus alleviating inflammation and intestinal damage in colitis. This study provided a useful approach to identify active components and the anticolitis mechanism of the FLCWK capsule and built up a reliable foundation for its clinical treatment.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"2948965"},"PeriodicalIF":2.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution and Blood Penetration of Hirudin in Various Organs and Tissues of Rabbits With Carotid Artery Injury by Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry. 超高效液相色谱-串联质谱法研究水蛭素在颈动脉损伤家兔各器官组织中的分布及血液渗透。

IF 2.3 3区化学

Journal of Analytical Methods in Chemistry Pub Date : 2025-05-02 eCollection Date: 2025-01-01 DOI: 10.1155/jamc/5644566

Yiran Feng, Lin Yang, Chunxia Guo, Ganyu Deng, Yudong Rao, Hao Zhou, Ying Zhang, Xueya Zhang

{"title":"Distribution and Blood Penetration of Hirudin in Various Organs and Tissues of Rabbits With Carotid Artery Injury by Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry.","authors":"Yiran Feng, Lin Yang, Chunxia Guo, Ganyu Deng, Yudong Rao, Hao Zhou, Ying Zhang, Xueya Zhang","doi":"10.1155/jamc/5644566","DOIUrl":"https://doi.org/10.1155/jamc/5644566","url":null,"abstract":"Background: Hirudin is a major active ingredient of the traditional Chinese medicine leech. It has been proved to have good antithrombotic and anticoagulant effects. Objective: To determine the tissue distribution of hirudin and its pharmacokinetics in vivo by ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods: A total of 21 SPF adult New Zealand rabbits were acclimatized for 1 week, and one was randomly selected as a blank control, while the remaining 18 were randomized to the control group and the model group. The heart, liver, spleen, lung, kidney, brain, plasma, blood vessels, and colon tissues were taken by execution at 1, 3, and 6 h. The samples were assayed using the natural hirudin standard as an external standard. Results: The established UPLC-MS/MS method with good precision, accuracy, recovery, and stability of hirudin proved to be reliable. The correlation between different concentrations of natural hirudin standard and the response value was R 2 ≥ 0.9997. The results of the distribution of various tissues showed that hirudin in the two groups had the highest content in plasma and blood vessels, followed by spleen, lung, and liver tissues, and was weaker in the brain and the heart, and the content of hirudin in the control group was higher than that of the model group, and the difference was statistically significant. Conclusion: The absorption and metabolism of hirudin in rabbits with carotid artery injury mainly acted through vascular tissues and blood, and normal rabbits mainly metabolized it through spleen, lung, and liver.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"5644566"},"PeriodicalIF":2.3,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Index Quantitative Analysis of Pharyngiyan Tablet Characteristics by Screening Different Quality Control Components. 咽咽炎片质量控制成分筛选的多指标定量分析。

IF 2.3 3区化学

Journal of Analytical Methods in Chemistry Pub Date : 2025-04-25 eCollection Date: 2025-01-01 DOI: 10.1155/jamc/6691730

Huan Cao, Liang Zhang, Xiaoning Zhan, Nan Hu, Xueyan Bi, Yanhong Wang

{"title":"Multi-Index Quantitative Analysis of Pharyngiyan Tablet Characteristics by Screening Different Quality Control Components.","authors":"Huan Cao, Liang Zhang, Xiaoning Zhan, Nan Hu, Xueyan Bi, Yanhong Wang","doi":"10.1155/jamc/6691730","DOIUrl":"https://doi.org/10.1155/jamc/6691730","url":null,"abstract":"Pharyngiyan tablet is a traditional Chinese medicine (TCM) renowned for its efficacy in moisturizing the lungs, clearing the throat. However, it exhibits quality variations due to discrepancies in regulatory standards and challenges in comprehensive evaluation. In this study, ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) was used to qualitatively analyze the chemical composition of pharyngiyan tablets. Full composition analysis detected 131 chemical constituents. Gallic acid, paeoniflorin, rutin, baicalin, and harpagoside were identified as quality control (QC) indexes through rational screening. These indexes were selected based on their effectiveness in demonstrating differences, stability after processing, specificity, and cost-effectiveness in measurement. For the multimetric quality assessment of the five screening components, a high-performance liquid chromatography with diode array detector (HPLC-DAD) method with wavelength switching was developed. A total of 104 batches of samples from 10 manufacturers were included in the analyses. The content of each ingredient varied significantly across enterprises and batches, with certain enterprises characterized by inferior drug feeding, deviation from prescribed ingredient amounts, and nonstandardized feeding practices. Overall, aquality assessment method was established to provide a basis for the market regulation and quality assessment of pharyngiyan tablets.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"6691730"},"PeriodicalIF":2.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of RP-HPLC Method for Simultaneous Determination of Triclabendazole and Ivermectin in Pharmaceutical Suspension Dosage Form. 反相高效液相色谱法同时测定药物悬浮液剂型中三氯咪唑和伊维菌素的含量。

IF 2.3 3区化学

Journal of Analytical Methods in Chemistry Pub Date : 2025-04-22 eCollection Date: 2025-01-01 DOI: 10.1155/jamc/5522915

Taj Ur Rahman, Ajmal Zaman, Ali Bahadur, Muhammad Aurang Zeb, Wajiha Liaqat, Eman Y Santali, Sarah Alharthi, Ruwida M K Omar, Saif A Alharthy, Ashraf Ali

{"title":"Development of RP-HPLC Method for Simultaneous Determination of Triclabendazole and Ivermectin in Pharmaceutical Suspension Dosage Form.","authors":"Taj Ur Rahman, Ajmal Zaman, Ali Bahadur, Muhammad Aurang Zeb, Wajiha Liaqat, Eman Y Santali, Sarah Alharthi, Ruwida M K Omar, Saif A Alharthy, Ashraf Ali","doi":"10.1155/jamc/5522915","DOIUrl":"https://doi.org/10.1155/jamc/5522915","url":null,"abstract":"A reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for simultaneous determination of triclabendazole (TCB) and ivermectin (IVM) in pharmaceutical dosage form. A mobile phase consisting of acetonitrile/water (50:50 v/v) with a flow rate of 1.5 mL/min was used for chromatographic separation of the mixture of TCB and IVM. The developed method was found to be linear with the correlation coefficient (r = 0.999) for TCB and IVM in the presence of suspension. The limit of quantitation (LOQ), robustness, specificity, accuracy, and precision were validated for the developed method. The peak areas of five replicates of the samples were recorded, and the acceptance rate of suspension recovery was 98%. The intraday accuracies for TCB and IVM were 98.71% and 100.79%, respectively, with a relative standard deviation (RSD) of 0.87%. The limits of detection (LOD) of TCB and IVM were 0.058 mg/mL and 0.112 μg/mL, respectively, while the LOQ of TCB and IVM were 0.178 μg/mL and 0.340 μg/mL, respectively. The method's % RSD for intra- and interday precision was deemed satisfactory. The developed method could be utilized for the determination and measurement of TCB and IVM in other samples.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2025 ","pages":"5522915"},"PeriodicalIF":2.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0