Journal of Analytical Methods in Chemistry最新文献

{"title":"A Fluorescence-Based High-Throughput Screening Method for EGFR Inhibitors Using an EGFR-Targeted Environment-Sensitive Fluorescent Probe.","authors":"Li-Xia Wang, Xiang-Dan Cuan, Xian-Yun Chen, Xuan-Jun Wang","doi":"10.1155/jamc/9333993","DOIUrl":"10.1155/jamc/9333993","url":null,"abstract":"<p><p>Fluorescence technology offers a highly sensitive, rapid, and efficient approach for drug screening at the cellular level, enabling the direct quantification of drug-target binding interactions through precise measurement of fluorescence intensity changes. In our previous research, a small-molecule fluorescent probe targeting epidermal growth factor receptor (EGFR) was successfully developed, featuring strong targeting specificity, low background signal, and an \"off-on\" response feature. Based on the EGFR-overexpressing A431 cell line, this study established a cell-based screening system for EGFR inhibitors and successfully identified flavokawain A (FKA), a novel potential EGFR inhibitor. FKA notably reduces the expression levels of <i>p</i>-EGFR and its downstream molecule <i>p</i>-Erk in the EGFR signaling pathway. It demonstrates a potent inhibitory effect on the proliferation of A431 cells, effectively suppressing clone formation and cellular migration, and exhibits a strong apoptosis-inducing effect against A431 cells. This fluorescence-based high-throughput screening method has demonstrated its effectiveness in identifying novel EGFR inhibitors from a wide range of natural materials. It holds significant application potential and plays a crucial role in expediting the discovery of EGFR inhibitors from medicinal sources.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9333993"},"PeriodicalIF":2.1,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13732097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149334014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Active Constituents and Underlying Mechanisms of Renshen Yangying Decoction Against Angina Pectoris Based on Integrated Serum Pharmacochemistry, Network Pharmacology, Molecular Docking, and Experimental Validation.","authors":"Ya Huang, Tao Xiong, Haixia Qiu, Rui Cheng, Yule Luo, Xingdong Wu","doi":"10.1155/jamc/6325730","DOIUrl":"10.1155/jamc/6325730","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to elucidate the pharmacological basis and underlying molecular mechanisms of Renshen Yangying decoction (RYD) in alleviating angina pectoris.</p><p><strong>Methods: </strong>UHPLC-Q-TOF-MS was used to profile chemical constituents in RYD and RYD-medicated serum. Compound identification was performed using a custom database, literature, reference standards, and the GNPS platform. Network pharmacology predicted disease-related targets and pathways, followed by molecular docking and in vitro AC16 cardiomyocyte assays for validation.</p><p><strong>Results: </strong>A total of 179 constituents in the decoction and 37 absorbed prototype compounds (mainly flavonoids, organic acids, alkaloids, and coumarins) were identified. 282 shared targets with angina were obtained, yielding 10 core targets (e.g., AKT1, TNF, BCL2, STAT3, and EGFR). Four main compounds (naringenin, schisantherin B, schisandrol A, and quercetin) were predicted to modulate EGFR, IGF-1R, and PDGFR-β pathways. Naringenin was experimentally confirmed to alleviate pathological damage by suppressing inflammatory responses.</p><p><strong>Conclusion: </strong>RYD exerts antiangina effects through a multicomponent, multitarget, multipathway mechanism involving EGFR, PI3K-Akt, and PDGFR-β signaling, with naringenin, schisantherin B, schisandrol A, and quercetin as major active constituents. These findings provide mechanistic insights supporting the clinical application of RYD.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"6325730"},"PeriodicalIF":2.1,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13728912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149321467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Portable, Low-Cost Spectrophotometer for Absorbance and Selected-Wavelength Fluorescence Measurements.","authors":"Sanam Pudasaini, Prakash Dangal, Rajesh Singh Rathore, Beni B Dangi, Vijay Singh Chhetri","doi":"10.1155/jamc/9371485","DOIUrl":"https://doi.org/10.1155/jamc/9371485","url":null,"abstract":"<p><p>We present a portable, low-cost, modular spectrophotometric platform designed for wavelength-selective optical measurements using interchangeable visible LEDs, an AS7343 multichannel spectral sensor, and Arduino-based data acquisition. The system was developed to provide a compact alternative for optical absorbance and fluorescence measurements while maintaining performance comparable to conventional laboratory instrumentation. The device was evaluated using aqueous solutions of copper (II) sulfate (CuSO₄) and potassium permanganate (KMnO₄), and results were benchmarked against a standard UV-Vis spectrophotometer. The system demonstrated concentration-dependent absorbance responses consistent with the Beer-Lambert law, with strong calibration linearity (<i>R</i> ² = 0.99 for CuSO₄ and <i>R</i> ² = 0.97 for KMnO₄). In addition, the platform was adapted for fluorescence measurements of Rhodamine B via a simple reconfiguration to a 90° excitation-emission geometry. The fluorescence response showed linear behavior over the 0-4-μM range, yielding an <i>R</i> ² value of 0.97. The results indicate that the proposed system provides reliable optical measurements for both absorbance and fluorescence modalities, offering a flexible and cost-effective alternative for analytical spectrophotometry applications in resource-limited laboratory settings.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9371485"},"PeriodicalIF":2.1,"publicationDate":"2026-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13717223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149297615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Evaluation of Ethylene Glycol Monopiperate as a Novel Antihyperlipidemic Prodrug: Synthesis, Antihyperlipidemic Activity, and Pharmacokinetics.","authors":"Guirong Borijihan, Mimi Liu, Mingming Bao, Zhengkun Sun, Meng He, Youxin Li","doi":"10.1155/jamc/3258325","DOIUrl":"https://doi.org/10.1155/jamc/3258325","url":null,"abstract":"<p><p>Piperine, a major active component isolated from the traditional Mongolian medicine <i>Piper longum L.</i>, exhibits antihyperlipidemic activity but also demonstrates inherent toxicity. In this study, ethylene glycol monopiperate (EGM), a piperine derivative, was synthesized via concentrated H₂SO₄-catalyzed esterification. Its structure was systematically characterized by FTIR, MS, ¹³C NMR, and ¹H NMR, with a yield of 60% and a purity of 99.2%. Subsequently, the in vitro cytotoxicity of EGM on HepG2 cells was evaluated, and the results indicated that over 90% cell viability was observed at concentrations up to 150 μg/mL. It was also investigated in male Wistar rats, and a sensitive, rapid, and accurate solid-phase extraction (SPE)-UPLC method was developed and validated for the quantification of EGM in rat plasma. The novel method showed good linearity over the concentration range of 1-16 µg/mL, a limit of detection (LOD) of 1 μg/mL, and high accuracy and precision (relative error [RE%] < 15%, relative standard deviation [RSD%] < 10%). In terms of antihyperlipidemic efficacy, EGM demonstrated excellent performance in reducing total cholesterol (TC), glucose (GLU), and low-density lipoprotein cholesterol (LDL-C), as well as elevating high-density lipoprotein cholesterol (HDL-C); these effects were notably better than the conventional control drug simvastatin. Pharmacokinetic analysis revealed that the plasma concentration-time profile of EGM followed a first-order open one-compartment model. Calculations using WINNONLIN software (ver. 5.2) showed a half-life (<i>t</i> _1/2) of 1.46 ± 0.67 h and an area under the curve (AUC₀-∞) of 7,15,530 ± 522.4 ng h/mL. EGM was stable in plasma over 24 h at room temperature and one month at -20°C, after three freeze-thaw cycles. These results verified that EGM may serve as a promising candidate for an antihyperlipidemic prodrug.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"3258325"},"PeriodicalIF":2.1,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13711490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149287047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Profile of Indigo Naturalis in Rat Plasma, Bile, Urine, and Feces by UPLC-Q-TOF MS Method.","authors":"Guozhe Zhang, Jianting Liu, Dan Yuan, Yiyu Qin","doi":"10.1155/jamc/8592761","DOIUrl":"https://doi.org/10.1155/jamc/8592761","url":null,"abstract":"<p><p>Indigo naturalis (IN, Qing-Dai) is a well-established traditional Chinese medicine (TCM) with extensive biological activities, particularly in the clinical management of ulcerative colitis (UC) and psoriasis. However, the comprehensive in vivo metabolic behavior of IN extract remains inadequately characterized. This research employed ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS) methodology to comprehensively examine the metabolic characteristics of IN in rat models. Biological samples, including plasma, bile, urine, and feces, were obtained from rats following single oral administration of IN extract. Sample preparation techniques involved protein precipitation, solid-phase extraction, and ultrasonic extraction for processing plasma, bile, urine, and feces specimens, respectively. Through precise mass measurement and fragment ion analysis, we identified 24 prototype compounds and 37 metabolites and clarified the transformation relationships between prototypes and their metabolites. The biotransformation pathways of IN components in rats primarily involved N- or O-glucuronidation, N- or O-sulfation, hydroxylation, dehydroxylation, demethylation, demethoxylation, decarbonylation, and reduction reactions. Our findings demonstrate that N-glucuronidation and N-sulfation represent the predominant metabolic reactions. This investigation marks the first systematic report on metabolite identification and metabolic pathway speculation of major constituents following oral administration of IN extract, providing a scientific foundation for understanding its in vivo processes.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"8592761"},"PeriodicalIF":2.1,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13711370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149287010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Analysis of Boron Trichloride Using ¹¹B Quantitative Nuclear Magnetic Resonance Spectroscopy (qNMR).","authors":"Daniel Van Niekerk, Jaspaul Tatlay, Hilaire V Kemami Wangun, Nicholas J Morehouse","doi":"10.1155/jamc/4351793","DOIUrl":"https://doi.org/10.1155/jamc/4351793","url":null,"abstract":"<p><p>Boron trichloride (BCl₃) is highly reactive and degrades rapidly when exposed to moisture, complicating analyses, and posing safety risks under standard laboratory conditions. To address these challenges, quantitative ¹¹B nuclear magnetic resonance (qNMR) spectroscopy has been introduced as an analytical method to accurately quantitate boron trichloride in solution. This technique involves the reaction of boron trichloride with methanol to produce trimethyl borate, which is then quantified using an external standard. The preparation process allows for the reaction and sample preparation to occur in a single step within the same vial, ensuring a safe, fast, and reliable analysis. The ¹¹B qNMR method was validated, demonstrating linearity (0.02 M to 0.50 M with <i>r</i> = 0.9999), accuracy (recovery of 100.05%), precision (inter- and intraday), stability, and robustness. This paper highlights the effectiveness of the ¹¹B qNMR method for the accurate, reliable, fast, and safe quantification of boron trichloride in organic solvents such as dichloromethane.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"4351793"},"PeriodicalIF":2.1,"publicationDate":"2026-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13695890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149267762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Analytical Methods for <i>N-</i>Nitrosamine Analysis as Impurities in Drug Substances and Drug Products.","authors":"Rimadani Pratiwi, Alifa Dhia Shalima, Mutakin, Aliya Nur Hasanah","doi":"10.1155/jamc/9550913","DOIUrl":"https://doi.org/10.1155/jamc/9550913","url":null,"abstract":"<p><p><i>N</i>-nitrosamine (hereinafter nitrosamine) is among the organic impurities characterized by high potency as genotoxic and probable human carcinogens found in drug substances and drug products. This type of impurity has become a major concern in pharmaceutical industry, since one of nitrosamine type of impurities, NDMA, was detected in valsartan active pharmaceutical ingredients back in 2018. As a result, the development of sensitive, selective, and reliable analytical methods is essential to maintain regulatory compliance and safeguard of patient health. This review provides an overview of recent analytical strategies in 2020-2025 for detecting nitrosamine impurities in pharmaceutical substances and products, with an emphasis on chromatography-based techniques and groundbreaking analytical approaches. Gas chromatography (GC) and liquid chromatography (LC) coupled with mass spectrometry are the predominant methods, offering high sensitivity and selectivity for quantifying trace levels of these impurities. GC-based techniques are most appropriate for volatile and thermally stable nitrosamines, whereas LC-MS methods are suitable for polar, nonvolatile, thermally unstable, and structurally complex nitrosamines, including nitrosamine drug substance-related impurities (NDSRIs). Additionally, alternative LC-based detectors, such as ultraviolet (UV) and fluorescence detection (FLD), as well as total nitrosamine assays, chemiluminescence, and colorimetric methods, are evaluated as complementary or cost-effective analytical options. Key analytical considerations, including sample preparation, ionization techniques, matrix effects, derivatization strategies, and method validation, are also examined. Furthermore, this review highlights current challenges and future directions in nitrosamine analysis.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9550913"},"PeriodicalIF":2.1,"publicationDate":"2026-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13688712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149267732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracing Hydrazine in Pharmaceuticals: Toxicological Concerns, Extraction Approaches, and Analytical Method Development.","authors":"Buhlebenkosi Mercy Donga, Rimadani Pratiwi, Aliya Nur Hasanah","doi":"10.1155/jamc/9609425","DOIUrl":"10.1155/jamc/9609425","url":null,"abstract":"<p><p>Hydrazine (N₂H₄) is a synthetic reagent that is used in the manufacturing of various active pharmaceutical ingredients (APIs) such as isoniazid, hydralazine, and carbidopa. The major concerns associated with its presence in medications as a residual reagent or degradation product arise because of its ability to damage the genetic material, cause mutations, and potentially trigger cancer. It has been classified under Class 2 genotoxic impurity (GTI), based on the ICH M7 guideline. Its multitissue toxicities including hepatotoxicity, neurotoxicity, and hematotoxicity have led to tight regulatory limits with the EMA threshold of toxicological concern (TTC) at 1.5 µg/day. Detecting hydrazine in trace concentrations is challenging because of its low molecular weight, high polarity, absence of chromophores, and chemical instability. In order to provide a unified framework for impurity profiling, we combine hydrazine's toxicological profiles, formation pathways, and analytical advancements. Recent advances on the extraction, derivatization, and analytical determination of hydrazine to improve its stability and detectability in pharmaceutical matrices ranging from 2020 to 2025 were collected. Comparative evaluation of the methodologies reveals that combining chemical derivatization with hyphenated techniques delivers the highest sensitivity. GC-MS/MS stands out as the gold standard, providing exceptionally low detection limits at the sub-ppm level with high precision and reliability, while UHPLC-fluorescence serves as an alternative with considerable sensitivity for detecting hydrazine. Gaps in method harmonization, reference standard availability, and validation for complex formulations still remain therefore future work should focus more on standardized analytical methods, development of greener methods of derivatization, as well as use of high-resolution detection systems.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9609425"},"PeriodicalIF":2.1,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Phytochemical Profiles and Bioactivity-Constituent Relationships in Seven <i>Pandanus</i> Species From Vietnam.","authors":"Do Hoang Giang, Le Thanh Huong, Hoang Thuy Duong, Nguyen Thu Uyen, Nguyen Tien Dat, Nguyen Hai Dang","doi":"10.1155/jamc/6373690","DOIUrl":"10.1155/jamc/6373690","url":null,"abstract":"<p><p>The genus <i>Pandanus</i> comprises numerous tropical plant species that are widely used in traditional medicine and local diets, yet comprehensive comparative studies linking their phytochemical composition to biological activities remain limited, particularly for underexplored species. In this study, forty-five methanolic extracts obtained from seven <i>Pandanus</i> species (<i>Pandanus tectorius, Pandanus amaryllifolius, Pandanus odoratissimus, Pandanus affinis, Pandanus nanofrutex, Pandanus humilis,</i> and <i>Pandanus tonkinensis</i>) collected in Vietnam were systematically evaluated for their phytochemical profiles and bioactivities. Total phenolic (TPC), flavonoid (TFC), saponin (TSC), and alkaloid (TAC) contents were quantified, and antioxidant activities (DPPH and hydroxyl radical scavenging) together with <i>α</i>-amylase inhibitory effects were assessed. Pronounced interspecific variation, together with selected exploratory organ-related patterns, was observed, with <i>P. tectorius</i> exhibiting consistently high phenolic, flavonoid, and saponin contents and the strongest antioxidant activities, while <i>P. amaryllifolius</i> was distinguished by exceptionally high alkaloid levels. To elucidate the relationships between chemical constituents and bioactivities, partial least squares regression (PLSR) and Spearman's correlation analyses were applied. The PLSR models showed strong explanatory and predictive performance (<i>Q</i> ² = 0.889), indicating that phenolic content showed the strongest association with free radical scavenging activities, whereas saponin content was most strongly associated with <i>α</i>-amylase inhibition. These patterns were consistent with the Spearman correlation analysis and the PLS biplot. Overall, this study provides an integrated phytochemical-bioactivity framework for <i>Pandanus</i> species, highlights distinct associations between metabolite classes and the measured bioactivities, and identifies underexplored taxa as promising candidates for future phytochemical and pharmacological investigations.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"6373690"},"PeriodicalIF":2.1,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13676034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frontiers in Neurotransmitter Probes: Innovations and Perspectives in Norepinephrine Detection Technology.","authors":"Rufei Ma, Zhao Wang","doi":"10.1155/jamc/5515088","DOIUrl":"10.1155/jamc/5515088","url":null,"abstract":"<p><p>Norepinephrine (NE), the primary neurotransmitter of locus coeruleus projections, plays a crucial role in regulating arousal, emotion, memory, and stress responses. Its dysregulation is closely associated with various neurological disorders such as depression, Parkinson's disease, and epilepsy. However, conventional detection techniques such as microdialysis coupled with liquid chromatography are constrained by limited spatiotemporal resolution and inherent invasiveness, largely due to NE's low concentration, rapid metabolism, and complex distribution in vivo. These limitations make real-time, in situ dynamic monitoring particularly challenging. In recent years, fluorescence and bioluminescence probe technologies, founded on molecular recognition or self-assembly strategies, have enabled notable advances in the in situ detection and imaging of NE, owing to their superior spatiotemporal resolution, favorable biocompatibility, and versatile design. This review summarizes several representative studies in the field, covering probe design principles, response mechanisms, analytical performance, and biological applications. It also highlights emerging frontiers such as subcellular localization, in vivo dynamic imaging, millisecond-scale monitoring, multitarget cooperative recognition, and integrated diagnosis and therapy. These technologies have deepened our understanding of the spatiotemporal dynamics of NE in physiological and pathological processes, while also providing powerful tools for mechanistic research, early diagnosis, and precise treatment of neuropsychiatric disorders.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"5515088"},"PeriodicalIF":2.1,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13676032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}