Journal of Analytical Methods in Chemistry最新文献

{"title":"QTOF and Orbitrap Technologies in Modern Pesticide Analysis: Applications in Residue Detection and Degradation Pathway Elucidation.","authors":"Ruilong Li, Liu Min, Kaili Gao, Liqun Zhong, Xingang Meng, Yue Cheng","doi":"10.1155/jamc/7886371","DOIUrl":"10.1155/jamc/7886371","url":null,"abstract":"<p><p>QTOF and Orbitrap techniques, as advanced mass spectrometry tools, show great potential in the field of pesticide residue analysis and degradation product identification. This paper reviews the application of these two techniques in pesticide residue analysis and degradation product identification. The principles and characteristics of QTOF and Orbitrap techniques are first introduced, including the advantages of high resolution, high sensitivity and high accuracy. Then, the applications of these techniques in different types of samples, including agricultural products, environmental samples and air samples, were explored. These studies demonstrate that QTOF and Orbitrap techniques can effectively detect and characterize low levels of pesticide residues and degradation products in complex samples. In addition, this paper summarizes the advantages and limitations of these techniques and suggests directions for future research. Taken together, the QTOF and Orbitrap techniques have important application prospects in pesticide residue analysis and degradation product identification and will provide strong support in areas such as food safety and environmental protection.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"7886371"},"PeriodicalIF":2.1,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13329766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148390491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Analysis of Flavonols and Their Glycosides and Gastrointestinal Transformation of Flavonol Glycosides in Yinxing Mihuan Oral Solution.","authors":"Yun Lyu, Wenqian Cheng, Kejing Niu, Shaoqing He, Lulu Ma, Jing Yang, Huijuan Yu","doi":"10.1155/jamc/1287338","DOIUrl":"10.1155/jamc/1287338","url":null,"abstract":"<p><p>Yinxing Mihuan Oral Solution (YMOS) is extensively utilized in the clinical treatment of cardiovascular and cerebrovascular diseases (CCVDs), with flavonols and their glycosides identified as the primary active ingredients. Currently, there are relatively few established quantitative methods available for analyzing flavonols and their glycosides, and the research regarding their transformation patterns under simulated gastrointestinal conditions is still limited. In this study, network pharmacology was employed to predict the active compounds and targets of YMOS associated with cardiovascular and cerebrovascular protection. A comprehensive network integrating compounds, targets, bioactivities, and CCVDs was meticulously constructed for 10 flavonol glycosides and their aglycones. It revealed that these compounds exert their blood circulation-promoting and blood stasis-eliminating effects by modulating 20 key targets, highlighting their therapeutic potential in CCVDs treatment. Furthermore, we quantitatively analyzed the 10 targeted flavonols and their glycosides in YMOS using ultra-performance liquid chromatography (UPLC), demonstrating consistent flavonoid levels across all tested samples. This method was also employed to investigate biotransformation rules between flavonol glycosides and their aglycones in vitro. Notably, it was observed that flavonol glycosides undergo a deglycosylation reaction in the rat's intestinal microbial culture medium, which was witnessed in a relatively stable manner under the simulated artificial gastrointestinal fluid. These findings provide important scientific insights into the mechanism of action and the quality control of YMOS.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"1287338"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13320375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148368376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Affordable Arsenic Detection Device Using Handmade Lokta Paper for Decentralized and Sustainable Manufacturing.","authors":"Ramdeep Shrestha, Bhanu Bhakta Neupane, Basant Giri","doi":"10.1155/jamc/9918530","DOIUrl":"10.1155/jamc/9918530","url":null,"abstract":"<p><p>The reliance on centralized manufacturing systems often shows vulnerability of global supply chains, as evidenced by disruptions during the COVID-19 pandemic and geopolitical conflicts. This study reports an affordable and portable paper strip sensor for arsenic detection in groundwater, developed using distributed manufacturing and sustainability principles. Handmade Lokta Paper (HLP), produced in the laboratory from bark of a native Lokta plant, <i>Daphne</i>, through eco-friendly methods, served as the sensor substrate. The HLP strip employed the Gutzeit reaction and integrated smartphone imaging for qualitative, semiquantitative, and quantitative arsenic analyses. The assay optimization was achieved with 1% (w/v) HgBr₂ impregnation, 5-min dipping time, 15-min arsine exposure, and imaging within 25 min. Testing of 21 groundwater samples from Nepal revealed strong agreement between the HLP strip and a commercial kit Econo-Quick (EQ) (Cohen's kappa = 0.811). The HLP strip demonstrated a lower limit of detection (18 vs. 20 µg/L) and limit of quantification (54 vs. 61 µg/L). Most water samples had arsenic concentrations below 10 µg/L, with few reaching Nepal's regulatory limit of 50 µg/L. In addition to robust analytical performance, the HLP strip exemplifies the scalability and cost-effectiveness of arsenic sensors, with a production cost of USD 0.37 per test-significantly lower than commercial alternatives. This innovation may foster local economic growth, minimize supply chain dependency, and reduce environmental impact through the sustainable utilization of Lokta paper. The study highlights the potential of integrating traditional materials with advanced analytical techniques to address global health and environmental challenges while promoting regional resilience and sustainability.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9918530"},"PeriodicalIF":2.1,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13197832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESSION OF CONCERN: Synthesis of Iron Oxide Nanoparticle Functionalized Activated Carbon and Its Applications in Arsenic Adsorption.","authors":"Journal Of Analytical Methods In Chemistry","doi":"10.1155/jamc/9762942","DOIUrl":"10.1155/jamc/9762942","url":null,"abstract":"","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9762942"},"PeriodicalIF":2.1,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13192401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Performance Liquid Chromatography Method Optimization and Validation for Potassium Bromate Detection in Bread Using a Quality-by-Design Approach.","authors":"Tesfamichael Gebretsadikan, Mebrahtom Gebrelibanos Hiben, Kalayou Hiluf Gebremedhin, Mulaynesh Zebrehe, Hayelom Fisseha, Teklu Hailu Abay","doi":"10.1155/jamc/9966003","DOIUrl":"10.1155/jamc/9966003","url":null,"abstract":"<p><strong>Background: </strong>Potassium bromate (KBrO₃) is a flour additive that strengthens dough and increases bread volume. However, the International Agency for Research on Cancer (IARC) has labeled it as a possible human carcinogen. Despite being banned in many countries, KBrO₃ is still found in bread, particularly in resource-limited settings like Ethiopia, which can pose serious health risks.</p><p><strong>Objective of study: </strong>The goal of this study is to develop and validate a sensitive, selective, and accurate high-performance liquid chromatography (HPLC) method for detecting and measuring KBrO₃ in bread using a quality by design (QbD) approach.</p><p><strong>Methodology: </strong>A reverse-phase HPLC method was developed and optimized using the central composite design (CCD) expert. Chromatographic analysis was performed on a C18 column with a mobile phase consisting of acetonitrile and phosphate buffer. The method was validated following the ICH Q2(R1) guidelines. Fifteen commercial bread samples were collected from each subcity of Mekelle and analyzed using the developed method.</p><p><strong>Results: </strong>The developed method demonstrated excellent linearity, precision, and accuracy, with recovery rates between 96% and 99%. The limit of detection (LOD) was 0.015 mg/kg. Bromate residues were detected in all bread samples, with concentrations ranging from 4.5 to 9.15 mg/kg, which is significantly higher than the WHO/FDA safety limit of 0.02 mg/kg.</p><p><strong>Conclusion: </strong>The validated HPLC method provides a reliable and accurate tool for routine monitoring of KBrO₃ in bread. The results highlight significant public health risks associated with excessive bromate use in local bread production and call attention to the urgent need for strengthened regulatory controls and enforcement of food safety standards in Ethiopia.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9966003"},"PeriodicalIF":2.1,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13189449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147987408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Detection of Tetracycline Residues in Milk Using Colorimetric Sensor Based on Polymethylmethacrylate (PMMA) and Polystyrene (PS) Polymers.","authors":"Rimadani Pratiwi, Putri Nur Azizah, Aliya Nur Hasanah","doi":"10.1155/jamc/5412774","DOIUrl":"10.1155/jamc/5412774","url":null,"abstract":"<p><p>In this study, the colorimetric approach is applied on an indicator strip for tetracycline detection in milk residue. Indicator strips made from PMMA-concentrated H₂SO₄ (9:1), PMMA-Mecke (9:1), and PMMA-Marquis (9:1) are the best compositions that can detect tetracycline within 10 s. The test results using scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDS) and infrared spectrophotometer showed that the reagent had entered the PMMA-based indicator strip. The limit of quantification (LOQ) and limit of detection (LOD) were 11.64 ppm and 3.49 ppm, respectively. The indicator strip has high selectivity toward tetracycline and is stable for 60 days at room temperature in a desiccator. The indicator strip detects tetracycline in milk samples with an accuracy of 83.24%-99.66%. The accuracy test results using liquid chromatography-tandem mass spectrometry (LC-MS/MS) also observed. The indicator strip may be appropriate as an initial screening tool for high-level tetracycline contamination; however, its utility is limited for purposes of strict regulatory compliance.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"5412774"},"PeriodicalIF":2.1,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13189459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147987453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breath by Breath: Navigating the Landscape of Exhaled Breath in Clinical Research and Prospects for the Future.","authors":"Nisha Parikh, Hetansh Rao, Akshada Dabhade, Vishwa Patel, Vikalp Shroff, Deep Prajapati, Palak Parikh, Ketan Ranch, B Mahalakshmi, Shery Jacob, Sai H S Boddu","doi":"10.1155/jamc/9959007","DOIUrl":"10.1155/jamc/9959007","url":null,"abstract":"<p><p>Exhaled breath analysis (EBA) has evolved as a noninvasive diagnostic technique with enormous promise in healthcare. The development in this area is based on the concept that the breathalyzers employed to measure the alcohol concentration in the blood can be utilized to evaluate health by analyzing the intricate chemical makeup of exhaled breath, which includes various volatile organic compounds (VOCs). Breath analysis could be the driver of personalized medicine and used in early disease identification as it involves the detection of a variety of disease-specific biomarkers. In diagnostics, EBA offers a noninvasive procedure of illness identification and monitoring. This approach allows for early detection, personalized treatment options, and therapeutic monitoring by detecting disease-specific VOC biomarkers such as acetone in diabetes and ethane in lipid peroxidation. Also, EBA can measure environmental exposures such as air pollution or occupational dangers. By identifying VOCs from various sources, researchers can measure exposure levels and assess related health hazards. Advances in the analytical methods, including mass spectrometry, gas chromatography, and sensor arrays made it possible to quantify and identify VOCs in exhaled air with high sensitivity and precision. This review focuses on the biomarkers present in breath and their role in identifying diseases. It covers the essential compounds found in breath and their biochemical processes. Furthermore, an in-depth examination of different methods for identifying biomarkers in breath and the factors influencing VOC concentrations, including age, storage conditions, external sources, and the difference between dead space air and alveolar air, has been discussed.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"9959007"},"PeriodicalIF":2.1,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13147357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Free Testosterone Chemiluminescence Detection Kit and Its Clinical Application.","authors":"Shuang Han, Min Liu, Simin Zhao, Wenjun Yan, Zhixian Lu, Xuan Huang","doi":"10.1155/jamc/5165899","DOIUrl":"10.1155/jamc/5165899","url":null,"abstract":"<p><strong>Background: </strong>Free testosterone (FT), the bioactive form comprising 1%-2% of total testosterone, directly enters cells. Unlike total testosterone, FT levels are less affected by sex hormone-binding globulin and better reflect biological activity. Accurate serum FT measurement is crucial for diagnosing conditions like male hypogonadism, PCOS, metabolic syndrome, osteoporosis, and Alzheimer's. However, existing methods suffer from inadequate sensitivity, complexity, and high cost, necessitating improved detection technologies. We developed and evaluated a chemiluminescence assay kit for FT.</p><p><strong>Methods: </strong>FT was quantified using a competitive immunoassay where sample FT competes with acridinium ester-labeled testosterone derivatives for binding to biotinylated antitestosterone antibodies on magnetic beads. Key parameters (magnetic bead concentration, biotinylation, labeling, and antibody/derivative concentrations) were optimized. Kit performance was rigorously assessed for linearity, limit of blank (LoB), accuracy, precision, stability, specificity, and clinical relevance. FT levels were measured in 1615 male and 2035 female patient samples to analyze clinical significance.</p><p><strong>Results: </strong>The assay demonstrated excellent linearity (<i>r</i> > 0.99), low LoB (0.021 pg/mL), high accuracy (deviation < 5%), precision (CV < 5%), and 12-month stability. Specificity testing showed no cross-reactivity. Method comparison with 392 clinical samples yielded a strong correlation (<i>r</i> = 0.9941). Analysis of patient samples revealed significant FT level differences among males with various diagnoses: lower levels in prostate cancer patients and higher levels in conditions like hair loss.</p><p><strong>Conclusion: </strong>The developed chemiluminescence FT assay kit exhibits superior performance, low cost, and high automation, fully meeting clinical requirements. FT measurement provides a valuable reference for diagnosing and assessing specific diseases, aiding improved clinical management.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"5165899"},"PeriodicalIF":2.1,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147772080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of Potential Antidepressant Active Ingredients of <i>Albiziae Flos</i> via HS-GC-MS, Chemometrics, and Network Pharmacology.","authors":"Dan Yang, Dan He, Yiwu Wang, Yuan Shen, Jialing Yu, Ruijia Yang, Lin Yang","doi":"10.1155/jamc/2885034","DOIUrl":"10.1155/jamc/2885034","url":null,"abstract":"<p><p><i>Albiziae Flos</i> (AF) is a traditional Chinese medicine with an extensive historical background. This study presented an integrated approach combining headspace-gas chromatography-mass spectrometry (HS-GC-MS), chemometrics, and network pharmacology to comprehensively evaluate the volatile components of AF and explore their potential antidepressant mechanisms. A total of 34 volatile compounds were identified through HS-GC-MS analysis. Fingerprint assessment revealed high consistency among 16 batches (similarity: 0.790-0.998), while chemometric analysis successfully discriminated samples from different geographical origins. Network pharmacology screening identified 15 active components and 131 potential targets, revealing multicomponent, multitarget characteristics of AF's antidepressant effects. Molecular docking simulations demonstrated strong binding affinity between linalool oxide configurations and 12 core targets, primarily through hydrogen bonding and hydrophobic interactions. This work lies in its comprehensive investigation of AF's volatile components using an integrated analytical-pharmacological approach, providing both a methodological framework for quality assessment and mechanistic insights for antidepressant drug development. Our findings established scientific foundations for quality control of AF and revealed its potential antidepressant mechanisms through multiple pathways.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"2885034"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12950218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Colorimetric Multimetabolite Assay for Quantitative Measurement of Keto Acids in Urine for At-Home Monitoring of Metabolic Disorders.","authors":"Dipanjan Bhattacharyya, Abby Kropielnicki, Brian L Lee, Yeganeh Khaniani, Marcia A LeVatte, David S Wishart","doi":"10.1155/jamc/4116313","DOIUrl":"10.1155/jamc/4116313","url":null,"abstract":"<p><p>Inborn errors of metabolism such as phenylketonuria (PKU) and maple syrup urine disease (MSUD) can cause severe developmental problems. Both conditions can lead to harmful levels of keto acids in biofluids-phenylpyruvic acid (PPA) in PKU and branched-chain α-keto acids in MSUD. Monitoring urinary keto acids helps track dietary adherence and reduces the risk of metabolic crisis. However, current at-home tests are qualitative and difficult to interpret, while existing metabolomic assays require expensive equipment and must be conducted in a lab. This study aimed to develop a simple, quantitative, rapid, at-home assay for detecting multiple urinary keto acids associated with PKU and MSUD. A modified two-step 2,4-dinitrophenylhydrazine (DNPH)-based multimetabolite assay was developed, where sodium hydroxide (NaOH) converts the yellow hydrazone precipitate to a stable amber solution, enabling quantification of multiple keto acids (700-7200 μM) within 10 min. The assay was validated using spiked urine samples and adapted into a prototype at-home kit using caprolactam-immobilized NaOH. Nuclear magnetic resonance (NMR)-based metabolomics was used as a reference method to authenticate readings from a PKU patient. Correlation studies demonstrated strong linearity for MSUD (<i>R</i> ² = 0.91-0.96)- and PKU (<i>R</i> ² = 0.95-0.99)-spiked samples. Quantification of keto acids in authentic PKU urine samples showed excellent agreement with the results of quantitative NMR-based metabolomics assays (<i>R</i> ² = 0.99). Low-cost, at-home colorimetric tests for urinary keto acids could enable screening, detection, and monitoring of PKU and MSUD in the 90% of the world without access to advanced metabolic clinics.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":"2026 ","pages":"4116313"},"PeriodicalIF":2.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12949077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147326034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}