Hong Ngoc Pham, Chieu An Tran, Thi Diep Trinh, Ngoc Lan Nguyen Thi, Huynh Nhu Tran Phan, Van Nhan Le, Ngoc Hung Le, Van Trung Phung
{"title":"UHPLC-Q-TOF-MS/MS Dereplication to Identify Chemical Constituents of <i>Hedera helix</i> Leaves in Vietnam.","authors":"Hong Ngoc Pham, Chieu An Tran, Thi Diep Trinh, Ngoc Lan Nguyen Thi, Huynh Nhu Tran Phan, Van Nhan Le, Ngoc Hung Le, Van Trung Phung","doi":"10.1155/2022/1167265","DOIUrl":"https://doi.org/10.1155/2022/1167265","url":null,"abstract":"<p><p><i>Hedera helix</i> has been reported to contain a wide range of metabolites and produce many pharmacological effects. This research demonstrates the determination and evaluation of the phytochemical profiling of <i>H. helix</i> grown in central Vietnam. Methanolic extract of ivy had been analyzed by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS). MS, and MS/MS experiments were manipulated using both negative and positive ionization modes to provide molecular mass information and production spectra for the structural elucidation of compounds. A total of 46 compounds including 24 triterpene saponins and other compounds were successfully identified of which four established saponin structures have been reported for the first time. This study has provided a base for building a quality control of the raw materials according to the profile of triterpene saponins and assessment of pharmaceutical ingredients of <i>H. helix</i> planted in Vietnam.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40619720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An Electrochemical-Based Point-of-Care Testing Methodology for Uric Acid Measurement.","authors":"Yuetong Zhao, Xia Song","doi":"10.1155/2022/8555842","DOIUrl":"https://doi.org/10.1155/2022/8555842","url":null,"abstract":"<p><p>Point-of-care technology (POCT) is an important method in clinical testing in the future, which can achieve the purpose of rapid analysis. In this work, we assembled an electrochemical POC sensor for uric acid (UA) by surface modification of a screen-printed electrode. Copper nanowires were used as electrode modifiers to achieve high-performance electrochemical oxidation of UA. This electrochemical sensor can achieve linear detection of UA in the range of 10 <i>μ</i>M to 2 mM. The detection limit of the sensor was calculated to be 2 <i>μ</i>M. Although the detection performance of this sensor is not competitive with high-performance electrochemical sensors, it has been able to meet the needs of POC detection. At the same time, the sensor has excellent anti-interference performance. It has also been used successfully to test urine and serum samples from healthy and gout patients.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40577039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lihua Li, Wenzhi Zhang, Yan Wei, Lizhen Yu, Dexiang Feng
{"title":"A Sensitive Fluorescent Immunoassay for Prostate Specific Antigen Detection Based on Signal Amplify Strategy of Horseradish Peroxidase and Silicon Dioxide Nanospheres.","authors":"Lihua Li, Wenzhi Zhang, Yan Wei, Lizhen Yu, Dexiang Feng","doi":"10.1155/2022/6209731","DOIUrl":"https://doi.org/10.1155/2022/6209731","url":null,"abstract":"<p><p>A simple, sensitive, and fluorescent immunoassay for the detection of prostate-specific antigen (PSA) based on horseradish peroxidase and silicon dioxide nanospheres as a signal amplification strategy has been described. In the design, the primary antibody (Ab<sub>1</sub>) of PSA was first immobilized on the 96-well plates via physical adsorption between polystyrene and hydrophobic groups of the antibody molecule. The silicon dioxide nanospheres (SiO<sub>2</sub> NSs), with large surface area and good biocompatibility, were loaded with horseradish peroxidase (HRP) and horseradish peroxidase-labeled secondary antibodies (HRP-Ab<sub>2</sub>) as signal amplification systems. In the presence of PSA, a sandwich-type immunocomplex composed of Ab<sub>1</sub>-Ag-HRP-Ab<sub>2</sub> had been formed. Fluorescence technology was employed to obtain the response signal of the immunoassay in the <i>L</i>-tyrosine and H<sub>2</sub>O<sub>2</sub> systems. Experiment results showed that a strong and stable fluorescent signal at 416 nm (excitation wavelength: 325 nm) was observed, and the changes in fluorescent intensity were related to the levels of PSA. Under the optimal conditions, the relative fluorescence intensity was linear with the logarithm of PSA concentration from 0.03 to 100 ng·mL<sup>-1</sup>, with a detection limit of 0.01 ng·mL<sup>-1</sup> (at a signal/noise ratio of 3). In contrast to other fluorescent immunoassays, the sandwich-type immunoreaction based on the high binding ELISA plates has the advantages of being simple, stable, and easy to operate, high selectivity, small sample quantity, etc., which can be widely used in the selective detection of a variety of targets, from DNA to proteins and small molecules. Such fluorescent immunoassays should be feasible for the fields of molecular diagnosis and other life science fields in the future.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40590925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marzieh Rahimi, Soleiman Bahar, S Mojtaba Amininasab
{"title":"Preparation of Molecular Imprinted Polymer Based on Chitosan as the Selective Sorbent for Solid-Phase Microextraction of Phenobarbital.","authors":"Marzieh Rahimi, Soleiman Bahar, S Mojtaba Amininasab","doi":"10.1155/2022/9027920","DOIUrl":"https://doi.org/10.1155/2022/9027920","url":null,"abstract":"<p><p>This study reports the construction of a novel SPME fiber based on chitosan and glutaraldehyde as coating material composites combined with high-performance liquid chromatography with an ultraviolet detector (HPLC-UV) for extraction and detection of phenobarbital. In this technique, the chitosan biopolymer, as a new coating of SPME fiber, was produced on the stainless-steel wire, using glutaraldehyde and phenobarbital as cross-linker and template, respectively. For comparison, a nonimprinted polymer was created using the same procedure to evaluate fiber selectivity (but without the addition of phenobarbital). The SPME-MIP fiber coating was characterized by field emission scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermal gravimetric analysis. The efficiency of fiber was then improved by adjusting the impact of numerous factors such as pH, extraction time, desorption time, desorption solvent, and stirring rate. The results showed that the proposed fiber has a linear range of 0.01-4 <i>μ</i>g·mL<sup>-1</sup>, and detection limit of 7.5 ng·mL<sup>-1</sup>. The average recoveries in the four concentration levels for the spiked river and well water samples were 95.7 and 95.3%, with relative standard deviations of 3.8 and 5.9% for single fiber and between fibers, respectively.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40644825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of Temperature Programmed Oxidation-Infrared Technique in the Analysis of Sulfur Occurrence and Genesis in Phosphate Rock.","authors":"Qiuyuan Xu, Shiyun Tang, Anjiang Tang, Yazhou Tian","doi":"10.1155/2022/3255760","DOIUrl":"https://doi.org/10.1155/2022/3255760","url":null,"abstract":"<p><p>In this study, a temperature programmed oxidation-infrared (TPO-IR) technique was improved and applied in the analysis of sulfur occurrence and genesis in phosphate rock. Phosphate rocks from three regions (KYP, ZJP, and WAP) were selected for the detection of sulfur species by TPO-IR combined with XRD, SEM, EDS, and XPS characterization. TPO-IR results show that the total sulfur contents of the three phosphate rocks were 2.14% for KYP, 1.18% for ZJP, and 1.06% for WAP. In the low-temperature area (<1000°C), TPO-IR detected that both KYP and WAP contain FeS with a characteristic temperature of about 513°C and their contents were 9.22‰ and 0.64‰, respectively. In high-temperature areas (>1000°C), the TPO-IR curves suggest that sulfate is the main sulfur species in the three phosphate rocks. Typically, the characteristic temperature near 1070<sup>o</sup>C belongs to MgSO<sub>4</sub>, and the characteristic temperature near 1290°C belongs to CaSO<sub>4</sub>. Due to the incomplete TPO-IR database of sulfur reference materials at present, it is not possible to assign all sulfur species in high-temperature areas. However, in a sense, this research provides theoretical basis and experimental support for the application of the TPO-IR technique for the detection of sulfur species in other solid minerals.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40622902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pharmacokinetic Study of Coadministration with Cefuroxime Sodium for Injection Influencing ReDuNing Injection-Derived Seven Phytochemicals and Nine Metabolites in Rats.","authors":"Qiulong Zhao, Chunxue Wang, Jiaxin Cheng, Hui Yan, Ling Wang, Dawei Qian, Jinao Duan","doi":"10.1155/2022/2565494","DOIUrl":"https://doi.org/10.1155/2022/2565494","url":null,"abstract":"<p><p>According to the sixth edition of China's \"New Coronavirus Diagnosis and Treatment Plan (NCDTP),\" ReDuNing injection (RDN) was firstly introduced to treat severe and critical COVID-19, whereas its combination with broad-spectrum antibiotics was suggested to take with extreme caution and full reasons. Therefore, we aim to describe the pharmacokinetics of seven active phytochemicals and semiquantification of nine relevant metabolites in ReDuNing injection (RDN) after combining with cefuroxime sodium (CNa) for injection in rat plasma. Male Sprague-Dawley rats were randomly assigned to six groups, and they were intravenously administered, respectively, with different prescriptions of RDN (2 mL/kg) and CNa (225 mg/kg). At different time points (0.03, 0.08, 0.17, 0.24, 0.33, 0.50, 0.67, 1, and 6 h) after administration, the drug concentrations of iridoids glycosides, organic acids, and metabolites in rat plasma were determined using ultrahigh-pressure liquid chromatography coupled with linear ion rap-orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap-MS), and main pharmacokinetic parameters were estimated by noncompartment model. The results showed that there were differences in pharmacokinetic parameters, AUC<sub>(0-t)</sub>, T<sub>1/2</sub>, <i>C</i> <sub>max</sub>, CL of iridoids glycosides, and organic acids, after the intravenous administration of the different combinations of RDN and CNa. Moreover, different combinations of the injections also resulted in different curves of relative changes of each metabolite. The obtained results suggested that RDN and CNa existed pharmacokinetic drug-herb interactions in rats. The findings not only lay the foundation for evaluating the safety of RDN injection combined with CNa but also make contributions to clinically applying RDN injection combined with CNa, which works potentially against severe forms of COVID-19.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40476661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quantitative Analysis of Multicomponents in Qufeng Zhitong Capsule and Application of Network Pharmacology to Explore the Anti-Inflammatory Activity of Focused Compounds.","authors":"Mengjie Xue, Yuting Zhao, Ying Cui, Jing Yang, Yuefei Wang, Xin Chai","doi":"10.1155/2022/4229945","DOIUrl":"https://doi.org/10.1155/2022/4229945","url":null,"abstract":"<p><p>Qufeng Zhitong capsule (QZC) is a well-known Chinese patent medicine that has been widely applied for the clinical treatment of rheumatoid arthritis and other inflammatory diseases. To date, its material basis is still unclear, which has greatly limited its clinical application. In this study, by taking advantage of ultra-high-performance liquid chromatography tandem <i>Q</i>-Exactive Orbitrap high-resolution mass spectrometry, 16 chemical components such as gallic acid, protocatechuic acid, and neochlorogenic acid in QZC were characterized and unambiguously identified based on comparison with the corresponding reference standards. In addition, the correlation between the focused components and their corresponding raw herbs from QZC prescription was investigated. For the first time, the relationship between the components mentioned above and their anti-inflammatory activity was explored via network pharmacology analysis, and a visualized network of \"medicinal materials-QZC-compounds-targets-pathways\" was established. Based on the brief prediction results of network pharmacological analysis, ultra-performance liquid chromatography coupled with photodiode array detector method was validated in terms of linearity, limit of detection, limit of quantification, precision, repeatability, stability, and recovery test and was successfully employed to determine 16 compounds in 28 batches of QZCs, which confirmed the feasibility and reliability of the established method for the quantitative analysis of 16 compounds in QZC. Considering the content and bioactivity of the tested components, four compounds were recommended as candidate indicators for quality evaluation ultimately. The potential value of this study could not only support a quality evaluation of QZC but also provide a theoretical basis for further in-depth research of QZC in clinical research.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40491533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass Spectrometry.","authors":"Chen Lan, Bing Zhao, Lu Yang, Yusen Zhou, Siyi Guo, Xuebin Zhang, Junli Zhang","doi":"10.1155/2022/7015311","DOIUrl":"https://doi.org/10.1155/2022/7015311","url":null,"abstract":"<p><p>Nucleotide sugars, the activated forms of monosaccharides, are important intermediates of carbohydrate metabolism in all organisms. Here, we describe a method for the detection and quantification of UDP-glucose and UDP-galactose in maize in order to compare their metabolism in both wild-type and mutated plants. Triple quadrupole operating in a multiple reaction monitoring mode was used to quantify nucleotide sugars. The limits of detection for UDP-glucose and UDP-galactose were 0.50 and 0.70 ng·mL<sup>-1</sup>, respectively. The recoveries of the method ranged from 98.3% to 103.6% with the relative standard deviations less than 6.3%. To prove the applicability of this method, we analyzed several sets of maize extracts obtained from different cultivars grown under standardized greenhouse conditions. All the results demonstrated the suitability of the developed method to quantify UDP-glucose and UDP-galactose in maize extracts.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40480210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuanyuan Wang, Zhijuan Meng, Chunyan Su, Sufang Fan, Yan Li, Haiye Liu, Xuan Zhang, Pingping Chen, Yunyun Geng, Qiang Li
{"title":"Rapid Screening of 352 Pesticide Residues in Chrysanthemum Flower by Gas Chromatography Coupled to Quadrupole-Orbitrap Mass Spectrometry with Sin-QuEChERS Nanocolumn Extraction.","authors":"Yuanyuan Wang, Zhijuan Meng, Chunyan Su, Sufang Fan, Yan Li, Haiye Liu, Xuan Zhang, Pingping Chen, Yunyun Geng, Qiang Li","doi":"10.1155/2022/7684432","DOIUrl":"https://doi.org/10.1155/2022/7684432","url":null,"abstract":"<p><p>To analyze pesticide residues, GC coupled with quadrupole-Orbitrap MS (GC-Orbitrap-MS) has become a powerful tool because of its unique characteristics of accurate mass full-spectrum acquisition, high resolution, fast acquisition rates, and overcoming matrix interference. This paper presents an efficiency evaluation of GC-Orbitrap-MS for identification and quantitation in the 352 pesticide residues analysis of chrysanthemum flowers in full-scan mode. A streamlined pretreatment approach using one-step extraction and dilution was used, which provided high-throughput processing and excellent recovery. The samples were extracted using acetonitrile. The extracted solution was purified by a Sin-QuEChERS Nano column to suppress the matrix in chrysanthemum flowers and determined by GC-Orbitrap-MS. The calibration curves for the 352 pesticides obtained by GC-Orbitrap-MS were linear in the range of 0.5-200 <i>μ</i>g·kg<sup>-1</sup>, with the correlation coefficients higher than 0.99. The limits of detection (LODs) and the limits of quantification (LOQs) for the 352 pesticide residues were 0.3-3.0 <i>μ</i>g·kg<sup>-1</sup> and 1.0-10.0 <i>μ</i>g·kg<sup>-1</sup>, respectively. The average recoveries in chrysanthemum flower at three levels were 95.2%, 88.6%, and 95.7%, respectively, with relative standard deviations (RSDs) of 7.1%, 7.5%, and 7.2%, respectively. Lastly, the validated method and retrospective analysis was applied to a total of 200 chrysanthemum flower samples bought in local pharmacies. The proposed method can simultaneously detect multipesticide residues with a good performance in qualitative and quantitative detection.</p>","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40402916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Snake Venom Analysis for Facilitating Wildlife Forensics: A Pilot Study","authors":"S. Bhargava, K. Kumari, R. Sarin, Rajvinder Singh","doi":"10.1155/2022/8644993","DOIUrl":"https://doi.org/10.1155/2022/8644993","url":null,"abstract":"Confirm and authentic identification of species is required for the implementation of wildlife laws in cases of illegal trafficking of snake venoms. Illegally trafficked snake venom might be misidentified with other drugs of abuse, and sometimes, the species of venom-yielding snake cannot be verified. Snake venoms from medically important snake species, Naja naja and Daboia russelii, were procured from Irula Snake Catcher's Society, Tamil Nadu, India. Comparative analyses of both venoms were carried out using SDS-PAGE, LC-MS/MS, ICP-MS, and mtDNA analysis. The protein concentration of Naja naja and Daboia russelii venoms was 76.1% and 83.9%, respectively. SDS analysis showed a distinct banding pattern of both venoms. LC-MS/MS results showed proteins and toxins from 12 to 14 protein families in Naja naja and Daboia russelii venoms. Elemental analysis using ICP-MS showed a different profile of some elements in both venoms. mtDNA analysis of venoms using universal primers against Cyt b gene showed homology with sequence of Naja naja and Daboia russelii genes. The study proposed a template of various conventional and advanced molecular and instrumental techniques with their pros and cons. The template can be used by forensic science laboratories for detection, screening, and confirmatory analysis of suspected venoms of snakes. Clubbing of various techniques can be used to confirm the identification of species of snake from which the alleged venom was milked. The results can be helpful in framing charge-sheets against accused of illegal venom trafficking and can also be used to verify the purity and quality of commercially available snake venoms.","PeriodicalId":14974,"journal":{"name":"Journal of Analytical Methods in Chemistry","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82267357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}