Journal of Animal Science and Biotechnology最新文献

{"title":"Dietary glycyrrhizin enhances reproductive performance by improving intestinal microbiota, liver lipid metabolism and ovarian senescence in aged breeder hens","authors":"Zhenwu Huang, Huchuan Liu, Guangju Wang, Huan Ge, Yanru Shi, Jinghai Feng, Chunmei Li, Minhong Zhang","doi":"10.1186/s40104-025-01288-5","DOIUrl":"https://doi.org/10.1186/s40104-025-01288-5","url":null,"abstract":"The decline in reproductive performance of aged hens is mainly attributed to oxidative damage in reproductive organs, hepatic lipid metabolism disorders, and intestinal microbiota dysbiosis. Glycyrrhizin (GL) has been proven to enhance antioxidant capacity, regulate lipid metabolism and gut microbiota in mammals, but its efficacy in hens remains unclear. Hence, this study aimed to investigate whether dietary GL supplementation improves reproductive performance in hens during the late laying stage by modulating intestinal microbiota composition, hepatic lipid metabolism and ovarian antioxidant status. Dietary supplementation with 100 mg/kg GL significantly improved the egg production rate, egg quality, and hatching rate in aged breeder hens (P < 0.05). GL supplementation also increased the serum levels of HDL-C, TP and ALB, and enhanced the antioxidant capacity in both serum and ovary (P < 0.05). In addition, dietary GL elevated the serum progesterone (P4) levels by enhancing the transcription level of steroid synthesis key enzymes (CYP11A1 and 3β-HSD) in the ovary (P < 0.05). Dietary GL also promoted the synthesis and transport of vitellogenin (VTG) by upregulating the VTG-II (P < 0.05) and APOV1 (P = 0.077) expression levels in the liver, thereby increasing the number of grade follicles and small yellow follicles. Moreover, dietary GL enhanced hepatic fatty acid β-oxidation by upregulating PPARα and CPT-I (P < 0.05), and downregulating ACC expression levels (P < 0.05). In agreement, liver metabolomics analysis revealed that dietary GL supplementation significantly altered hepatic metabolism, with 389 differentially identified metabolites (P < 0.05). The key metabolites (e.g., taurocholic acid, tauroursodeoxycholic acid, nicotinuric acid, glycodeoxycholic acid (hydrate)) were identified, and they were mainly functionally enriched in beta-alanine metabolism nicotinate, taurine and hypotaurine metabolism (P < 0.05). Finally, 16S rRNA gene sequencing revealed that dietary GL reversed age-induced changes in gut microbiota composition, characterized by a significant increase in Lactobacillus abundance and a decrease in Bacteroides (P < 0.05). These results collectively demonstrate that dietary supplementation with 100 mg/kg GL improved reproductive performance by reversing age-induced changes in gut microbiota, enhancing hepatic vitellogenin synthesis, and ameliorating ovarian function in aged breeder hens. This study suggests that dietary GL is a potential strategy to improve reproductive performance in broiler breeder hens during the late laying period. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"14 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145554417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevotella copri alleviates diarrhea in weaning piglets through gut microbiota modulation and arachidonic acid–AHR–NRF2 pathway activation","authors":"Cong Lan, Wen Ren, Aimin Wu, Bing Yu, Jun He, Yuheng Luo, Daiwen Chen","doi":"10.1186/s40104-025-01273-y","DOIUrl":"https://doi.org/10.1186/s40104-025-01273-y","url":null,"abstract":"Diarrhea remains a major health concern in both young animals and humans. Prevotella spp., a dominant commensal genus in the healthy porcine gut, becomes increasingly abundant following weaning, suggesting a potential role during this critical transitional period. However, its involvement in post-weaning diarrhea remains poorly understood. Here, we aim to elucidate the role and underlying mechanisms of Prevotella in alleviating diarrhea in weaned piglets. To model unsanitary housing conditions, piglets were housed in uncleaned pens containing residual fecal matter from previous occupants and exposed to cold stress by maintaining the ambient temperature at 19 °C, below the optimal 28 °C. Under these conditions, piglets were orally administered either a blank medium (CON, n = 10 × 2) or Prevotella copri at 1 × 108 CFU (Pc, n = 10 × 2) on d 1, 3, and 5. After 28 d, cold stress induced a diarrhea incidence of 33.45% in the CON group, while P. copri supplementation significantly reduced the diarrhea rate to 19.73%. Treatment with P. copri markedly improved intestinal morphology in the small intestine, decreased serum levels of lipopolysaccharide (LPS) and intestinal fatty acid-binding protein (i-FABP), and enhanced total antioxidant capacity (T-AOC) and catalase (CAT) activity. Quantitative PCR and 16S rRNA gene sequencing revealed that P. copri significantly increased the colonic abundance of Prevotella, reshaping both the composition and functional profile of the gut microbiota. Moreover, P. copri enhanced the modularity and robustness of microbial ecological networks. Untargeted metabolomic profiling of colonic contents revealed a significant enrichment of metabolites involved in the arachidonic acid pathway following P. copri supplementation. In parallel, untargeted metabolomics of P. copri culture supernatants identified differential metabolic pathways including metabolic pathways, biosynthesis of secondary metabolites, and biosynthesis of antibiotics. In vitro assays demonstrated that P. copri-derived metabolites inhibited the growth of three common porcine intestinal pathogens. Furthermore, both P. copri metabolites and arachidonic acid enhanced intestinal barrier integrity and suppressed TNF-α-induced inflammation and apoptosis in Caco-2 cells through activation of the AHR–Nrf2 signaling pathway. These findings highlight the role of P. copri in maintaining gut homeostasis and provide new insights into microbiota-based interventions for early-life intestinal disorders. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145554671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chicken gut microbiome in conventional and alternative production systems","authors":"Yu-Chieh Cheng, Margret Krieger, Anna-Maria Korves, Amélia Camarinha-Silva","doi":"10.1186/s40104-025-01293-8","DOIUrl":"https://doi.org/10.1186/s40104-025-01293-8","url":null,"abstract":"The poultry gut microbiome plays a key role in nutrient digestion, immune function, and overall health. Differences among various farming systems, including conventional, antibiotic-free, free-range, and organic systems, influence microbial composition and function through variations in diet, genetic selection, environmental exposure, and antibiotic use. Conventional systems typically rely on formulated diets and controlled housing conditions, often with routine antimicrobial use. In contrast, organic systems emphasize natural feed ingredients, including roughage, outdoor access, and strict limitations on the use of antibiotics. These divergent practices shape the gut microbiota differently, with organic systems generally associated with greater exposure to environmental microbes and, consequently, greater microbial diversity. However, the implications of this increased diversity for poultry health and performance are complex, as organic systems may also carry a higher risk of pathogen exposure. This review summarizes current findings on the chicken gut microbiome across conventional and alternative production systems (antibiotic-free, free-range, and organic), focusing on microbial diversity, functional potential, and disease resilience. The need for standardized methodologies and consistent nomenclature in microbiome research is also discussed to improve comparability across studies. Understanding how production systems influence the gut microbiota is essential for improving poultry health and productivity while addressing challenges related to antimicrobial resistance and sustainable farming practices.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"13 1","pages":"153"},"PeriodicalIF":7.0,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145545823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in understanding the role of gut microbiota in fat deposition and lipid metabolism","authors":"Yi Zhong, Yuhang Lei, Shan Jiang, Dujun Chen, Xinyi Wang, Kai Wang, Tianci Liao, Rongjie Liao, Mailin Gan, Lili Niu, Ye Zhao, Lei Chen, Xiaofeng Zhou, Yan Wang, Li Zhu, Linyuan Shen","doi":"10.1186/s40104-025-01284-9","DOIUrl":"https://doi.org/10.1186/s40104-025-01284-9","url":null,"abstract":"The gut microbiota has emerged as a pivotal regulator of host lipid metabolism and energy homeostasis. A growing body of evidence reveals that variations in the composition and metabolic activity of intestinal microbes are closely associated with differences in adipose tissue deposition across species. Notably, increased abundance of Firmicutes and a reduced proportion of Bacteroidetes and butyrate-producing bacteria have been linked to enhanced fat accumulation. Key microbial metabolites such as short-chain fatty acids (SCFAs) influence lipid metabolism through multiple pathways, including the activation of GPR41/43 receptors, modulation of the bile acid–FXR/TGR5 axis, and regulation of hepatic lipogenesis. Additionally, the gut–brain axis plays a critical role in controlling feeding behavior via neuroendocrine signaling. This review summarizes current advances in understanding the roles of dominant bacterial phyla and beneficial genera—including Clostridium butyricum and Faecalibacterium prausnitzii—in fat metabolism. We further explore the mechanisms by which gut microbiota modulate lipid synthesis and catabolism through SCFA production, bile acid signaling, and AMPK/PPAR-related pathways. These insights highlight the potential of microbiota-targeted strategies to restore lipid metabolic balance, offering novel opportunities for applications in health management, nutritional interventions, and microbial therapeutics. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145535432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salvianolic acid C inhibits methane emissions in dairy cows by targeting MCR and reshaping the rumen microbial community","authors":"Zihao Liu, Li Xiao, Xiangfang Tang, Yue He, Xuemei Nan, Hui Wang, Yuming Guo, Benhai Xiong","doi":"10.1186/s40104-025-01285-8","DOIUrl":"https://doi.org/10.1186/s40104-025-01285-8","url":null,"abstract":"Methane (CH4) emissions from ruminants significantly contribute to greenhouse gas effects and energy loss in livestock production. Methyl-coenzyme M reductase (MCR) is the key enzyme in methanogenesis, making it a promising target for CH4 mitigation. This study aimed to identify and validate plant-derived inhibitors by using molecular docking to screen compounds with strong binding affinity to the F430 active site of MCR and assessing their efficacy in reducing CH4 emissions. Molecular docking analysis identified salvianolic acid C (SAC) as a potent inhibitor of MCR, showing a strong binding affinity to the F430 active site (binding energy: −8.2 kcal/mol). Enzymatic inhibition assays confirmed its inhibitory effect, with a half-maximal inhibitory concentration (IC50) of 692.3 µmol/L. In vitro rumen fermentation experiments demonstrated that SAC supplementation (1.5 mg/g DM) significantly reduced CH4 production (P < 0.01) without negatively affecting major fermentation parameters. Microbial community analysis using 16S rRNA sequencing and metagenomics revealed that SAC selectively altered the rumen microbiota, increasing the relative abundance of Bacteroidota while significantly reducing Methanobrevibacter (P = 0.04). Moreover, metagenomic analysis showed the downregulation of key methanogenesis-related genes (mcrA and rnfC), suggesting a dual mechanism involving direct enzymatic inhibition and microbial community modulation. These findings indicate that SAC effectively reduces CH4 production by inhibiting MCR activity and reshaping the rumen microbial community. As a plant-derived compound with strong inhibitory effects on methanogenesis, SAC presents a promising and sustainable alternative to synthetic CH4 inhibitors, offering potential applications for mitigating CH4 emissions in livestock production. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"49 1","pages":"151"},"PeriodicalIF":7.0,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145531727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing CRISPR-Cas9 for Lactobacillus improvement in silage production: current knowledge and future perspectives","authors":"Jing Ma, Jiao Zhang, Xusheng Guo","doi":"10.1186/s40104-025-01282-x","DOIUrl":"https://doi.org/10.1186/s40104-025-01282-x","url":null,"abstract":"High-quality silage is the cornerstone to sustainable livestock development and animal food production. As the core fermentation bacteria of silage, Lactobacillus directly regulates silage fermentation by producing lactic acid, enzymes, and other bioactive molecules. However, traditional screening methods for functional strains are labor-intensive and time-consuming. Recent advances in synthetic biology, particularly the development of CRISPR-Cas genome editing technology, offer a revolutionary approach to designing Lactobacillus strains with customized traits. This review systematically reviewed the importance of silage in sustainable agricultural development and the limitations of current silage preparation and promotion. It also discussed the application of strain engineering approaches in optimizing the phenotypic performance of Lactobacillus for better silage. Building on this, we reviewed the research progress of CRISPR-Cas9 gene editing in Lactobacillus and discussed how to leverage its high efficiency and precision to optimize the strain’s traits for improved silage quality and functionality. CRISPR-Cas9 toolkits are expected to achieve directed evolution of strain performance, ultimately yielding next-generation silage microbial inoculants with multiple functions, adaptability to multiple substrates, and eco-friendly characteristics. The use of such innovative biotechnologies would facilitate resource-efficient utilization, promote animal performance and health for sustainable development in livestock production. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145516068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new vision of Panax ginseng leaf polysaccharide function: multiple roles in improving growth, flesh quality and muscle energy metabolism of sub-adult grass carp (Ctenopharyngodon idella)","authors":"Jianrong Yang, Pei Wu, Weidan Jiang, Yang Liu, Yaobin Ma, Xiaowan Jin, Hongmei Ren, Hequn Shi, Xiaoqiu Zhou, Lin Feng","doi":"10.1186/s40104-025-01256-z","DOIUrl":"https://doi.org/10.1186/s40104-025-01256-z","url":null,"abstract":"As living standards improve, consumers are placing greater emphasis on the enhancement of fish flesh quality, making its improvement increasingly critical. Plant-derived polysaccharides positively affect the improvement of animal flesh quality. Panax ginseng leaf polysaccharides (PGLP) have a similar composition and lower cost compared with Panax ginseng root polysaccharides. However, its function and application effects in grass carp (Ctenopharyngodon idella) are unclear. A total of 540 sub-adult grass carp (679 ± 1.29 g), one of the important economic fish species, were used as experimental models and fed diets supplemented with 0, 100, 200, 300, 400, or 500 mg/kg PGLP for 60 d. After 60 d, grass carp were weighed, and their muscles were collected to explore the effects of PGLP on the growth and development of myofibers and energy metabolism-related parameters. Our study found that PGLP increased the growth performance and muscle nutritional composition as well as improved muscle hardness, springiness, cohesiveness, chewiness, and hyperplasia of myofibers of sub-adult grass carp. Besides, PGLP promoted muscle energy metabolism by increasing creatine, glycogen, pyruvate, and acetyl-CoA contents and creatine kinase (CK), pyruvate kinase (PK), phosphofructokinase (PFK), and hexokinase (HK) activities, while decreasing lactate dehydrogenase (LDH) activity and lactate content in fish muscle. Finally, our study found that PGLP enhanced mitochondrial function by increasing the protein expression of mitochondrial complexes I–V, biogenesis, and fusion and decreasing autophagy and fission in fish muscle. PGLP improved growth performance and flesh quality of sub-adult grass carp, which may be related to enhancing hyperplasia of myofibers by promoting energy metabolism. We concluded that the recommended amount of PGLP in sub-adult grass carp feed to optimize growth performance is 100–200 mg/kg. This study provides a theoretical basis for the application of PGLP in fish feed and for the analysis of the mechanism of nutrition and feed regulating fish flesh quality, which is of great significance. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"102 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145509494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Succinic acid-driven gut-fat axis orchestrates abdominal fat deposition in chickens via adipocyte-macrophage crosstalk","authors":"Jiahui Chen, Chuang Hu, Yu Wang, Lin Qi, Haoqi Peng, Genghua Chen, Qinghua Nie, Xiquan Zhang, Wen Luo","doi":"10.1186/s40104-025-01278-7","DOIUrl":"https://doi.org/10.1186/s40104-025-01278-7","url":null,"abstract":"Excessive abdominal fat in broilers not only reduces feed efficiency and increases processing costs but also raises environmental concerns. This pathological overaccumulation results from complex metabolic dysregulation across multiple organs. While current research largely centers on adipogenesis within adipose tissue, a comprehensive understanding of the cross-organ regulatory factors influencing this process remains elusive. Here, we employed a high-fat diet (HFD) model and multi-omics approaches to investigate cross-organ regulatory mechanisms underlying abdominal fat deposition in broilers. Our results demonstrated that HFD not only promoted fat accumulation but also altered meat quality traits. Through 16S rRNA amplicon sequencing, we identified significant gut microbiota dysbiosis in HFD-fed chickens, manifested by an increased abundance of Lactobacillus and a decreased abundance of Enterococcus. However, jejunal microbiota transplantation from HFD donors did not induce abdominal fat deposition in recipient chickens. Metabolomic profiling revealed that HFD elevated the level of succinic acid, a metabolite positively correlated with Lactobacillus abundance and potentially generated by Lactobacillus. This increase in succinic acid (SA) further triggered metabolic inflammation response in both jejunal tissue and serum. In vivo validation established succinic acid as a key inflammatory mediator facilitating HFD-induced cross-organ communication between the jejunum and abdominal adipose tissue, enhancing intestinal lipid uptake and subsequent abdominal fat deposition. Bulk and single-nucleus RNA sequencing (snRNA-seq) revealed that HFD induced macrophage population expansion and intensified adipocyte-macrophage crosstalk. Adipocyte-macrophage co-culture systems further elucidated that macrophages are an indispensable factor in succinic acid-induced fat deposition. This study delineates a succinic acid-driven \"gut-fat axis\" governing abdominal fat deposition in broilers, integrating gut microbiota dysbiosis and macrophage-mediated inflammatory adipogenesis. By identifying succinic acid as a cross-organ signaling molecule that enhances lipid absorption and activates macrophage-dependent adipogenesis, we establish systemic metabolic-immune crosstalk as a pivotal regulatory mechanism. These findings redefine fat deposition as a process extending beyond adipose-centric models, advancing multi-omics-guided strategies for sustainable poultry production.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"171 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145509543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in zearalenone-degrading enzymes research: characteristics, mining, improvement, and application","authors":"Yu Tang, Aimin Chen, Yongpeng Guo, Yanan Wang, Lihong Zhao","doi":"10.1186/s40104-025-01281-y","DOIUrl":"https://doi.org/10.1186/s40104-025-01281-y","url":null,"abstract":"Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin that extensively contaminates feed and feed ingredients, posing a significant threat to animal health and food safety. Enzymatic degradation of ZEN is regarded as a promising strategy due to its high efficiency and safety. This review provides a comprehensive summary of recent advances in ZEN-degrading enzymes from a novel perspective, encompassing the types and catalytic mechanisms for characterizing ZEN-degrading enzymes, the methods for mining ZEN-degrading enzymes, the strategies for improving ZEN-degrading enzymes, and the applications of ZEN-degrading enzymes. The objective of this review is to offer a reliable reference framework for the enzymatic detoxification of ZEN in feed and feed ingredients, as well as to provide insights for mining other mycotoxin degrading enzyme in the future.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"19 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145499478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota and metabolites in lipid metabolism and intramuscular fat deposition: mechanisms and implications for meat quality","authors":"Xiaofeng Song, Chenglong Jin, Ruifan Wu, Yongjie Wang, Xiaofan Wang","doi":"10.1186/s40104-025-01279-6","DOIUrl":"https://doi.org/10.1186/s40104-025-01279-6","url":null,"abstract":"Intramuscular fat (IMF) content serves as the key determinants of meat quality. Emerging evidence indicates that gut microbiota and their metabolites significantly influence IMF deposition levels by modulating host lipid metabolism through multiple pathways, positioning microbial regulation as a pivotal target for meat quality improvement. However, existing studies remain fragmented, predominantly focusing on isolated mechanisms or correlations without a systematic view of the regulatory network. This review consolidates the core mechanisms through which microbiota-derived metabolites including short-chain fatty acids, bile acids, branched-chain amino acids, trimethylamine N-oxide, tryptophan derivatives, succinate, polyamines etc., regulate IMF deposition and proposes a targeted intervention framework, the “gut microbiota/metabolites-IMF axis”. By integrating these insights, we provide a theoretical foundation and define practical research pathways to assess the potential of microbial-based strategies for improving meat quality in swine production.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"25 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145499477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}