Journal of Animal Science and Biotechnology最新文献

{"title":"Necroptosis contributes to deoxynivalenol-induced liver injury and inflammation in weaned piglets","authors":"Qilong Xu, Hanqiu Gong, Mohan Zhou, Junjie Guo, Shaokui Chen, Kan Xiao, Yulan Liu","doi":"10.1186/s40104-024-01117-1","DOIUrl":"https://doi.org/10.1186/s40104-024-01117-1","url":null,"abstract":"The aim of this study was to investigate the role of necroptosis in deoxynivalenol (DON)-induced liver injury and inflammation in weaned piglets. In Exp. 1, 12 weaned piglets were divided into 2 groups including pigs fed basal diet and pigs fed diet contaminated with 4 mg/kg DON for 21 d. In Exp. 2, 12 weaned piglets were divided into 2 groups including control piglets and piglets given a gavage of 2 mg/kg body weight (BW) DON. In Exp. 3, 24 weaned piglets were used in a 2 × 2 factorial design and the main factors including necrostatin-1 (Nec-1) (DMSO or 0.5 mg/kg BW Nec-1) and DON challenge (saline or 2 mg/kg BW DON gavage). On 21 d in Exp. 1, or at 6 h post DON gavage in Exp. 2 and 3, pigs were killed for blood samples and liver tissues. Liver histology, blood biochemical indicators, and liver inflammation and necroptosis signals were tested. Dietary or oral gavage with DON caused liver morphological damage in piglets. Dietary DON led to hepatocyte damage indicated by increased aspartate transaminase (AST) activity and AST/alanine aminotransferase (ALT) ratio, and DON gavage also caused hepatocyte damage and cholestasis indicated by increased AST and alkaline phosphatase (AKP) activities. Dietary DON caused liver necroptosis indicated by increased protein abundance of total receptor interacting protein kinase 3 (t-RIP3) and total mixed lineage kinase domain-like protein (t-MLKL). Moreover, DON gavage increased mRNA expression of interleukin (IL)-6 and IL-1β in liver. DON gavage also induced liver necroptosis demonstrated by increased protein abundance of t-RIP3, phosphorylated-RIP3 (p-RIP3), t-MLKL and p-MLKL. However, pretreatment with Nec-1, a specific inhibitor of necroptosis, inhibited liver necroptosis indicated by decreased protein expression of t-RIP3, p-RIP3, t-MLKL and p-MLKL. Nec-1 pretreatment reduced liver morphological damage after DON gavage. Pretreatment with Nec-1 also attenuated liver damage induced by DON indicated by decreased activities of AST and AKP. Furthermore, Nec-1 pretreatment inhibited liver mRNA expression of IL-6 and IL-1β after DON challenge. Our data demonstrate for the first time that necroptosis contributes to DON-induced liver injury and inflammation in piglets.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"198 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142759924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorogenic acid alleviates IPEC-J2 pyroptosis induced by deoxynivalenol by inhibiting activation of the NF-κB/NLRP3/caspase-1 pathway","authors":"Yanmei Xue, Fuchang Li, Rui Li, Xinru Zhang, Huijun Guo, Chunyang Wang","doi":"10.1186/s40104-024-01119-z","DOIUrl":"https://doi.org/10.1186/s40104-024-01119-z","url":null,"abstract":"Deoxynivalenol (DON) is a mycotoxin that severely pollutes feed ingredients, and methods for reducing DON toxicity have become a significant research direction. Chlorogenic acid (CGA) is an active polyphenol found in some plants, which has anti-inflammatory and antioxidant properties and a protective effect on animal intestinal health. The effects of CGA on DON-induced pyroptosis in the intestinal porcine epithelial cell line-J2 (IPEC-J2) and its potential mechanism were explored in this study. IPEC-J2 cells viability and membrane integrity were inversely correlated with DON concentration. Compared to those in the group treated with DON alone at 2,500 ng/mL, pretreatment with 80 μmol/L CGA for 4 h significantly improved cell viability (P < 0.01), and the alleviation of typical pyroptotic symptoms induced by DON were observed, including reduced cellular DNA fragmentation, decreased release of lactate dehydrogenase (LDH), normalized ROS levels, restoration of extracellular Ca2+ and K+ contents to normal levels (P < 0.01 ), as well as suppressed the enzyme activities of caspase-1 and caspase-4 (P < 0.01). Additionally, the mRNA expression levels of TNF, MDP, NOD2, TLR4, ASC and GSDMD were significantly improved (P < 0.01), while both mRNA and protein expression levels of NF-κB, NLRP3, caspase-1, IL-1β and IL-18 were significantly upregulated (P < 0.01) in the CGA + DON group, compare to those in the DON group. Pretreatment with 80 μmol/L CGA for 4 h effectively alleviated pyroptosis in IPEC-J2 cells induced by 2,500 ng/mL of DON through inhibiting activation of the NF-κB/ NLRP3/capase-1 pathway.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"3 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Dietary garcinol supplementation improves diarrhea and intestinal barrier function associated with its modulation of gut microbiota in weaned piglets","authors":"Tongxin Wang, Weilei Yao, Juan Li, Yafei Shao, Qiongyu He, Jun Xia, Feiruo Huang","doi":"10.1186/s40104-024-01134-0","DOIUrl":"https://doi.org/10.1186/s40104-024-01134-0","url":null,"abstract":"This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1186/s40104-020-0426-6.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"75 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of antioxidant capability by dietary N-acetyl cysteine supplementation alleviates bone loss induced by chronic heat stress in finisher broilers","authors":"Huaiyong Zhang, Herinda Pertiwi, Joris Michiels, Djoere Gaublomme, Maryam Majdeddin, Yuhuang Hou, Matthieu Boone, Dirk Elewaut, Iván Josipovic, Jeroen Degroote","doi":"10.1186/s40104-024-01114-4","DOIUrl":"https://doi.org/10.1186/s40104-024-01114-4","url":null,"abstract":"Heat stress (HS) incidence is associated with the accumulation of reactive substances, which might be associated with bone loss. N-Acetylcysteine (NAC) exhibits strong antioxidants due to its sulfhydryl group and being as the precursor for endogenous glutathione synthesis. Therefore, interplay between oxidative stress and bone turnover of broilers and the effects of dietary NAC inclusion on antioxidant capability and “gut-bone” axis were evaluated during chronic HS. Implementing cyclic chronic HS (34 °C for 7 h/d) evoked reactive oxygen species excessive production and oxidant stress, which was accompanied by compromised tibia mass. The RNA-seq of proximal tibia also revealed the enrichment of oxidation–reduction process and inflammatory outbursts during HS. Although no notable alterations in the growth performance and cecal microbiota were found, the diet contained 2 g/kg NAC enhanced the antioxidant capability of heat-stressed broiler chickens by upregulating the expression of Nrf2 in the ileum, tibia, and bone marrow. Simultaneously, NAC tended to hinder NF-κB pathway activation and decreased the mRNA levels of the proinflammatory cytokines in both the ileum and bone marrow. As a result, NAC suppressed osteoclastogenesis and osteoclast activity, thereby increasing osteocyte-related gene expression. Furthermore, the inclusion of NAC tended to increase the ash content and density of the whole tibia, as well as improve cortical thickness and bone volume of the diaphysis. These findings HS-mediated outburst of oxidant stress accelerates bone resorption and negatively regulates the bone quality of tibia, which is inhibited by NAC in broilers. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"203 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142756380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Embryotrophic effect of exogenous protein contained adipose-derived stem cell extracellular vesicles","authors":"Seonggyu Bang, Ahmad Yar Qamar, Sung Ho Yun, Na‑Yeon Gu, Heyyoung Kim, Ayeong Han, Heejae Kang, Hye Sun Park, Seung II Kim, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho","doi":"10.1186/s40104-024-01127-z","DOIUrl":"https://doi.org/10.1186/s40104-024-01127-z","url":null,"abstract":"&lt;p&gt;&lt;b&gt;Correction&lt;/b&gt;&lt;b&gt;: &lt;/b&gt;&lt;b&gt;J Animal Sci Biotechnol 15, 145 (2024)&lt;/b&gt;&lt;/p&gt;&lt;p&gt;&lt;b&gt;https://doi.org/10.1186/s40104-024-01106-4&lt;/b&gt;&lt;/p&gt;&lt;br/&gt;&lt;p&gt;Following publication of the original article [1], the authors reported an error in authors’ affiliations due to typesetting error, where the 3&lt;sup&gt;rd&lt;/sup&gt; and 4&lt;sup&gt;th&lt;/sup&gt; institutions are the same. Also, a full stop was erroneously added to author Seung II Kim’s name.&lt;/p&gt;&lt;p&gt;The full list of authors and affiliations is changed from:&lt;/p&gt;&lt;p&gt;Seonggyu Bang&lt;sup&gt;1,2&lt;/sup&gt;, Ahmad Yar Qamar&lt;sup&gt;3&lt;/sup&gt;, Sung Ho Yun&lt;sup&gt;5&lt;/sup&gt;, Na-Yeon Gu&lt;sup&gt;6&lt;/sup&gt;, Heyyoung Kim&lt;sup&gt;2,7&lt;/sup&gt;, Ayeong Han&lt;sup&gt;1,2&lt;/sup&gt;, Heejae Kang&lt;sup&gt;1,2&lt;/sup&gt;, Hye Sun Park&lt;sup&gt;4&lt;/sup&gt;, Seung II. Kim&lt;sup&gt;4&lt;/sup&gt;, Islam M. Saadeldin&lt;sup&gt;2,8&lt;/sup&gt;, Sanghoon Lee&lt;sup&gt;2&lt;/sup&gt; and Jongki Cho&lt;sup&gt;1&lt;/sup&gt;&lt;sup&gt;*&lt;/sup&gt;&lt;/p&gt;&lt;p&gt;1 College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea.&lt;/p&gt;&lt;p&gt;2 College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.&lt;/p&gt;&lt;p&gt;3 College of Veterinary and Animal Sciences, Jhang Sub-campus of University of Veterinary and Animal Sciences, Lahore, Pakistan.&lt;/p&gt;&lt;p&gt;4 College of Veterinary and Animal Sciences, Jhang, Sub-Campus of University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.&lt;/p&gt;&lt;p&gt;5 Korea Basic Science Institute (KBSI), Ochang, Chungcheongbuk‑Do 28119, Republic of Korea.&lt;/p&gt;&lt;p&gt;6 Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbuk‑Do 39660, Republic of Korea.&lt;/p&gt;&lt;p&gt;7 Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA)&lt;/p&gt;&lt;p&gt;Laboratory, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.&lt;/p&gt;&lt;p&gt;8 Comparative Medicine Department, King Faisal Specialist Hospital &amp; Research Centre, Riyadh 11211, Saudi Arabia.&lt;/p&gt;&lt;p&gt;To:&lt;/p&gt;&lt;p&gt;Seonggyu Bang&lt;sup&gt;1,2&lt;/sup&gt;, Ahmad Yar Qamar&lt;sup&gt;3&lt;/sup&gt;, Sung Ho Yun&lt;sup&gt;4&lt;/sup&gt;, Na‑Yeon Gu&lt;sup&gt;5&lt;/sup&gt;, Heyyoung Kim&lt;sup&gt;2,6&lt;/sup&gt;, Ayeong Han&lt;sup&gt;1,2&lt;/sup&gt;, Heejae Kang&lt;sup&gt;1,2&lt;/sup&gt;, Hye Sun Park&lt;sup&gt;4&lt;/sup&gt;, Seung II Kim&lt;sup&gt;4&lt;/sup&gt;, Islam M. Saadeldin&lt;sup&gt;2,7&lt;/sup&gt;, Sanghoon Lee&lt;sup&gt;2&lt;/sup&gt; and Jongki Cho&lt;sup&gt;1&lt;/sup&gt;&lt;sup&gt;*&lt;/sup&gt;&lt;/p&gt;&lt;p&gt;1 College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea.&lt;/p&gt;&lt;p&gt;2 College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.&lt;/p&gt;&lt;p&gt;3 College of Veterinary and Animal Sciences, Jhang Sub-campus of University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.&lt;/p&gt;&lt;p&gt;4 Korea Basic Science Institute (KBSI), Ochang, Chungcheongbuk‑Do 28119, Republic of Korea.&lt;/p&gt;&lt;p&gt;5 Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbuk‑Do 39660, Republic of Korea.&lt;/p&gt;&lt;p&gt;6 Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA)&lt;/p&gt;&lt;p&gt;Laboratory, Johns Hopkins ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"2675 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142753705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Dietary supplementation with pterostilbene activates the PI3K-AKT-mTOR signalling pathway to alleviate progressive oxidative stress and promote placental nutrient transport","authors":"Mingming Cao, Liyun Bai, Haoyun Wei, Yantong Guo, Guodong Sun, Haoyang Sun, Baoming Shi","doi":"10.1186/s40104-024-01124-2","DOIUrl":"https://doi.org/10.1186/s40104-024-01124-2","url":null,"abstract":"&lt;p&gt;&lt;b&gt;Correction&lt;/b&gt;&lt;b&gt;: &lt;/b&gt;&lt;b&gt;J Animal Sci Biotechnol 15, 133 (2024)&lt;/b&gt;&lt;/p&gt;&lt;p&gt;&lt;b&gt;https://doi.org/10.1186/s40104-024-01090-9&lt;/b&gt;&lt;/p&gt;&lt;br/&gt;&lt;p&gt;Following publication of the original article [1], the authors reported errors in the legend of Fig. 7 and the &lt;i&gt;P&lt;/i&gt; value of Fig. 8G (0.66 should be corrected to 0.066).\u0000&lt;/p&gt;&lt;p&gt;The originally published legend of Fig. 7 was:&lt;/p&gt;&lt;p&gt;Effect of PTE on milk composition, antioxidant capacity, inflammatory factors and immunoglobulins. &lt;b&gt;A&lt;/b&gt; Colostrum composition. &lt;b&gt;B&lt;/b&gt; Colostrum antioxidant capacity. &lt;b&gt;C&lt;/b&gt; Colostrum inflammatory factor levels. &lt;b&gt;D&lt;/b&gt; Colostrum immunoglobulin levels. &lt;b&gt;E&lt;/b&gt; Milk composition. &lt;b&gt;F&lt;/b&gt; Milk antioxidant capacity. &lt;b&gt;G&lt;/b&gt; Milk inflammatory factor levels. &lt;b&gt;H&lt;/b&gt; Milk immunoglobulin levels. CON: control group; PTE: Pterostilbene group. Data are expressed as mean ± SD (&lt;i&gt;n&lt;/i&gt; = 6 for each group). &lt;sup&gt;*&lt;/sup&gt;&lt;i&gt;P&lt;/i&gt; &lt; 0.05, compared to the control group.&lt;/p&gt;&lt;p&gt;The corrected legend of Fig. 7 should read:&lt;/p&gt;&lt;p&gt;Effect of PTE on milk composition, antioxidant capacity, inflammatory factors and immunoglobulins. &lt;b&gt;A&lt;/b&gt; Colostrum composition. &lt;b&gt;B&lt;/b&gt; Colostrum antioxidant capacity. &lt;b&gt;C&lt;/b&gt; Colostrum inflammatory factor levels and colostrum immunoglobulin levels. &lt;b&gt;D&lt;/b&gt; Milk composition. &lt;b&gt;E&lt;/b&gt; Milk antioxidant capacity. &lt;b&gt;F&lt;/b&gt; Milk inflammatory factor levels and milk immunoglobulin levels. CON: Control group; PTE: Pterostilbene group. Data are expressed as mean ± SD (&lt;i&gt;n&lt;/i&gt; = 6 for each group). &lt;sup&gt;*&lt;/sup&gt;&lt;i&gt;P&lt;/i&gt; &lt; 0.05, compared to the control group.&lt;/p&gt;&lt;p&gt;The originally published Fig. 8 was:&lt;/p&gt;&lt;figure&gt;&lt;figcaption&gt;&lt;b data-test=\"figure-caption-text\"&gt;Fig. 8&lt;/b&gt;&lt;/figcaption&gt;&lt;picture&gt;&lt;source srcset=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01124-2/MediaObjects/40104_2024_1124_Fig1_HTML.png?as=webp\" type=\"image/webp\"/&gt;&lt;img alt=\"figure 1\" aria-describedby=\"Fig1\" height=\"431\" loading=\"lazy\" src=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01124-2/MediaObjects/40104_2024_1124_Fig1_HTML.png\" width=\"685\"/&gt;&lt;/picture&gt;&lt;p&gt;Effect of PTE on fecal SCFAs in sows (&lt;b&gt;A&lt;/b&gt;–&lt;b&gt;G&lt;/b&gt;). CON: control group; PTE: Pterostilbene group. Data are expressed as mean ± SD (&lt;i&gt;n&lt;/i&gt; = 6 for each group). &lt;sup&gt;*&lt;/sup&gt;&lt;i&gt;P&lt;/i&gt; &lt; 0.05, compared to the control group&lt;/p&gt;&lt;span&gt;Full size image&lt;/span&gt;&lt;svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"&gt;&lt;use xlink:href=\"#icon-eds-i-chevron-right-small\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;/use&gt;&lt;/svg&gt;&lt;/figure&gt;&lt;p&gt;The corrected Fig. 8 should read:&lt;/p&gt;&lt;figure&gt;&lt;figcaption&gt;&lt;b data-test=\"figure-caption-text\"&gt;Fig. 8&lt;/b&gt;&lt;/figcaption&gt;&lt;picture&gt;&lt;source srcset=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01124-2/MediaObjects/40104_2024_1124_Fig2_HTML.png?as=webp\" type=\"image/webp\"/&gt;&lt;img alt=\"figure 2\" aria-describedby=\"Fig2\" height=\"483\" loading=\"lazy\" src=\"//media.springernature.com/lw685/springer-static/ima","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"256 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the dietary amino acid profiles and the relative biomarkers for amino acid balance in the low-protein diets for broiler chickens","authors":"Bin Wang, Xiaodan Zhang, Yongfa Liu, Mingkun Gao, Mi Wang, Yuan Wang, Xinzhi Wang, Yuming Guo","doi":"10.1186/s40104-024-01108-2","DOIUrl":"https://doi.org/10.1186/s40104-024-01108-2","url":null,"abstract":"Research on low-protein-level diets has indicated that even though the profiles of essential amino acids (EAAs) follow the recommendation for a normal-protein-level diet, broilers fed low-protein diets failed to achieve productive performance compared to those fed normal diets. Therefore, it is imperative to reassess the optimum profile of EAAs in low-protein diets and establish a new ideal pattern for amino acid balance. Furthermore, identifying novel sensitive biomarkers for assessing amino acid balance will greatly facilitate the development of amino acid nutrition and application technology. In this study, 12 dietary treatments [Con(+), Con(-), L&A(-), L&A(+), M&C(-), M&C(+), BCAA (-), BCAA(+), Thr(-), Thr(+), Trp(-) and Trp(+)] were established by combining different EAAs including lysine and arginine, methionine and cysteine, branched-chain amino acid (BCAA), threonine, and tryptophan to observe the growth and development of the broiler chickens fed with low-protein-level diets. Based on the biochemical parameters and untargeted metabolomic analysis of animals subjected to different treatments, biomarkers associated with optimal and suboptimal amino acid balance were identified. Growth performance, carcass characteristics, hepatic enzyme activity, serum biochemical parameters, and breast muscle mRNA expression differed significantly between male and female broilers under different dietary amino acid patterns. Male broilers exhibited higher sensitivity to the adjustment of amino acid patterns than female broilers. For the low-protein diet, the dietary concentrations of lysine, arginine, and tryptophan, but not of methionine, cystine, or threonine, needed to be increased. Therefore, further research on individual BCAA is required. For untargeted metabolomic analysis, Con(+) was selected as a normal diet (NP) while Con(-) represented a low-protein diet (LP). L&A(+) denotes a low-protein amino acid balanced diet (LPAB) and Thr(+) represents a low-protein amino acid imbalance diet (LPAI). The metabolites oxypurinol, pantothenic acid, and D-octopine in birds were significantly influenced by different dietary amino acid patterns. Adjusting the amino acid profile of low-protein diets is required to achieve normal growth performance in broiler chickens fed normal-protein diets. Oxypurinol, pantothenic acid, and D-octopine have been identified as potentially sensitive biomarkers for assessing amino acid balance.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"35 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriocin Microcin J25’s antibacterial infection effects and novel non-microbial regulatory mechanisms: differential regulation of dopaminergic receptors","authors":"Lijun Shang, Fengjuan Yang, Qingyun Chen, Ziqi Dai, Guangxin Yang, Xiangfang Zeng, Shiyan Qiao, Haitao Yu","doi":"10.1186/s40104-024-01115-3","DOIUrl":"https://doi.org/10.1186/s40104-024-01115-3","url":null,"abstract":"The antibacterial and immunomodulatory activities of bacteriocins make them attractive targets for development as anti-infective drugs. Although the importance of the enteric nervous system (ENS) in the struggle against infections of the intestine has been demonstrated, whether it is involved in bacteriocins anti-infective mechanisms is poorly defined. Here, we demonstrated that the bacteriocin Microcin J25 (J25) significantly alleviated diarrhea and intestinal inflammation in piglets caused by enterotoxigenic Escherichia coli (ETEC) infection. Mechanistically, macrophage levels were significantly downregulated after J25 treatment, and this was replicated in a mouse model. Omics analysis and validation screening revealed that J25 treatment induced significant changes in the dopaminergic neuron pathway, but little change in microbial structure. The alleviation of inflammation may occur by down-regulating dopamine receptor (DR) D1 and the downstream DAG-PKC pathway, thus inhibiting arachidonic acid decomposition, and the inhibition of macrophages may occur through the up-regulation of DRD5 and the downstream cAMP-PKA pathway, thus inhibiting NF-κB. Our studies’ findings provide insight into the changes and possible roles of the ENS in J25 treatment of ETEC infection, providing a more sophisticated foundational understanding for developing the application potential of J25.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"69 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary bile acids supplementation protects against Salmonella Typhimurium infection via improving intestinal mucosal barrier and gut microbiota composition in broilers","authors":"Dan Hu, Xiaoran Yang, Ming Qin, Li’an Pan, Haiyan Fang, Pengnan Chen, Yingdong Ni","doi":"10.1186/s40104-024-01113-5","DOIUrl":"https://doi.org/10.1186/s40104-024-01113-5","url":null,"abstract":"Salmonella Typhimurium (S. Typhimurium) is a common pathogenic microorganism and poses a threat to the efficiency of poultry farms. As signaling molecules regulating the interaction between the host and gut microbiota, bile acids (BAs) play a protective role in maintaining gut homeostasis. However, the antibacterial effect of BAs on Salmonella infection in broilers has remained unexplored. Therefore, the aim of this study was to investigate the potential role of feeding BAs in protecting against S. Typhimurium infection in broilers. A total of 144 1-day-old Arbor Acres male broilers were randomly assigned to 4 groups, including non-challenged birds fed a basal diet (CON), S. Typhimurium-challenged birds (ST), S. Typhimurium-challenged birds treated with 0.15 g/kg antibiotic after infection (ST-ANT), and S. Typhimurium-challenged birds fed a basal diet supplemented with 350 mg/kg of BAs (ST-BA). BAs supplementation ameliorated weight loss induced by S. Typhimurium infection and reduced the colonization of Salmonella in the liver and small intestine in broilers (P < 0.05). Compared to the ST group, broilers in ST-BA group had a higher ileal mucosal thickness and villus height, and BAs also ameliorated the increase of diamine oxidase (DAO) level in serum (P < 0.05). It was observed that the mucus layer thickness and the number of villous and cryptic goblet cells (GCs) were increased in the ST-BA group, consistent with the upregulation of MUC2 gene expression in the ileal mucosa (P < 0.05). Moreover, the mRNA expressions of Toll-like receptor 5 (TLR5), Toll-like receptor 4 (TLR4), and interleukin 1 beta (IL1b) were downregulated in the ileum by BAs treatment (P < 0.05). 16S rDNA sequencing analysis revealed that, compared to ST group, BAs ameliorated the decreases in Bacteroidota, Bacteroidaceae and Bacteroides abundances, which were negatively correlated with serum DAO activity, and the increases in Campylobacterota, Campylobacteraceae and Campylobacter abundances, which were negatively correlated with body weight but positively correlated with serum D-lactic acid (D-LA) levels (P < 0.05). Dietary BAs supplementation strengthens the intestinal mucosal barrier and reverses dysbiosis of gut microbiota, which eventually relieves the damage to the intestinal barrier and weight loss induced by S. Typhimurium infection in broilers.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"4 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens","authors":"Minghui Wang, Kelin Li, Hongchao Jiao, Jingpeng Zhao, Haifang Li, Yunlei Zhou, Aizhi Cao, Jianmin Wang, Xiaojuan Wang, Hai Lin","doi":"10.1186/s40104-024-01123-3","DOIUrl":"https://doi.org/10.1186/s40104-024-01123-3","url":null,"abstract":"&lt;p&gt;\u0000&lt;b&gt;Correction&lt;/b&gt;&lt;b&gt;: &lt;/b&gt;\u0000&lt;b&gt;J Animal Sci Biotechnol 15, 113 (2024)&lt;/b&gt;\u0000&lt;/p&gt;&lt;p&gt;&lt;b&gt;https://doi.org/10.1186/s40104-024-01071-y&lt;/b&gt;&lt;/p&gt;&lt;br/&gt;&lt;p&gt;Following publication of the original article [1], the authors reported a typo in the author name.&lt;/p&gt;&lt;p&gt;The author name &lt;b&gt;Jianming Wang&lt;/b&gt; should be corrected to &lt;b&gt;Jianmin Wang&lt;/b&gt;.&lt;/p&gt;&lt;p&gt;The original article [1] has been updated.&lt;/p&gt;&lt;ol data-track-component=\"outbound reference\" data-track-context=\"references section\"&gt;&lt;li data-counter=\"1.\"&gt;&lt;p&gt;Wang M, Li K, Jiao H, et al. Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens. J Animal Sci Biotechnol. 2024;15:113. https://doi.org/10.1186/s40104-024-01071-y.&lt;/p&gt;&lt;p&gt;Article CAS Google Scholar &lt;/p&gt;&lt;/li&gt;&lt;/ol&gt;&lt;p&gt;Download references&lt;svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"&gt;&lt;use xlink:href=\"#icon-eds-i-download-medium\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;/use&gt;&lt;/svg&gt;&lt;/p&gt;&lt;h3&gt;Authors and Affiliations&lt;/h3&gt;&lt;ol&gt;&lt;li&gt;&lt;p&gt;College of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China&lt;/p&gt;&lt;p&gt;Minghui Wang, Kelin Li, Hongchao Jiao, Jingpeng Zhao, Xiaojuan Wang &amp; Hai Lin&lt;/p&gt;&lt;/li&gt;&lt;li&gt;&lt;p&gt;College of Life Sciences, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China&lt;/p&gt;&lt;p&gt;Haifang Li&lt;/p&gt;&lt;/li&gt;&lt;li&gt;&lt;p&gt;College of Chemistry and Material Science, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China&lt;/p&gt;&lt;p&gt;Yunlei Zhou&lt;/p&gt;&lt;/li&gt;&lt;li&gt;&lt;p&gt;Shandong Longchang Animal Health Products Co., Ltd., Jinan, P. R. China&lt;/p&gt;&lt;p&gt;Aizhi Cao &amp; Jianmin Wang&lt;/p&gt;&lt;/li&gt;&lt;/ol&gt;&lt;span&gt;Authors&lt;/span&gt;&lt;ol&gt;&lt;li&gt;&lt;span&gt;Minghui Wang&lt;/span&gt;View author publications&lt;p&gt;You can also search for this author in &lt;span&gt;PubMed&lt;span&gt; &lt;/span&gt;Google Scholar&lt;/span&gt;&lt;/p&gt;&lt;/li&gt;&lt;li&gt;&lt;span&gt;Kelin Li&lt;/span&gt;View author publications&lt;p&gt;You can also search for this author in &lt;span&gt;PubMed&lt;span&gt; &lt;/span&gt;Google Scholar&lt;/span&gt;&lt;/p&gt;&lt;/li&gt;&lt;li&gt;&lt;span&gt;Hongchao Jiao&lt;/span&gt;View author publications&lt;p&gt;You can also search for this author in &lt;span&gt;PubMed&lt;span&gt; &lt;/span&gt;Google Scholar&lt;/span&gt;&lt;/p&gt;&lt;/li&gt;&lt;li&gt;&lt;span&gt;Jingpeng Zhao&lt;/span&gt;View author publications&lt;p&gt;You can also search for this author in &lt;span&gt;PubMed&lt;span&gt; &lt;/span&gt;Google Scholar&lt;/span&gt;&lt;/p&gt;&lt;/li&gt;&lt;li&gt;&lt;span&gt;Haifang Li&lt;/span&gt;View author publications&lt;p&gt;You can also search for this author in &lt;span&gt;PubMed&lt;span&gt; &lt;/span&gt;Google Scholar&lt;/span&gt;&lt;/p&gt;&lt;/li&gt;&lt;li&gt;&lt;span&gt;Yunlei Zhou&lt;/span&gt;View author publications&lt;p&gt;You can also search for this author in &lt;span&gt;PubMed&lt;span&gt; &lt;/span&gt;Google Scholar&lt;/span&gt;&lt;/p&gt;&lt;/li&gt;&lt;li&gt;&lt;span&gt;Aizhi Cao&lt;/span&gt;View author publications&lt;p&gt;You can also search for ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"69 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142598476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}