Journal of Animal Science and Biotechnology最新文献

{"title":"Synthesis and efficacy of tetraacetyl vitamin C ester: a novel alternative to traditional vitamin C and sodium acetate in aquafeeds","authors":"Yanqing Li, Min Fu, Lijuan Yu, Zhen Zhang, Zhiyong Xue, Lanhui Huang, Rolf Erik Olsen, Yuanyuan Yao, Yalin Yang, Chao Ran, Chen Wang, Qianwen Ding, Zhigang Zhou","doi":"10.1186/s40104-026-01394-y","DOIUrl":"https://doi.org/10.1186/s40104-026-01394-y","url":null,"abstract":"The application of traditional vitamin C (VC) and sodium acetate (SA) in aquafeeds is hampered by significant limitations. VC suffers from poor stability during feed processing and storage, as well as low bioavailability. SA is highly hygroscopic, thermally unstable, and rapidly absorbed, leading to short duration of action. This study aimed to design, synthesize, and evaluate tetraacetyl vitamin C ester (TVCE), a novel VC-acetic acid conjugate, as a superior alternative.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"21 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing genomic prediction for key production traits in chickens through ultrasound phenotyping and multi-model comparative analysis","authors":"Ranran Zhu, Yuxiang Jiang, Wanyi Xiong, Yu Zhang, Ziyi Lian, Danni Gou, Zhandeng Li, Xiuping Wang, Xuemei Deng","doi":"10.1186/s40104-026-01384-0","DOIUrl":"https://doi.org/10.1186/s40104-026-01384-0","url":null,"abstract":"Growth performance and carcass traits are economically vital in poultry breeding. In Wenchang chickens, reducing excessive abdominal fat represents a critical breeding objective. However, as a typical carcass trait, abdominal fat thickness has traditionally been measurable only post-slaughter, resulting in inefficient and costly selection processes that hinder genetic progress for these traits. To overcome this limitation, we developed an integrated approach combining non-invasive ultrasound phenotyping and multi-model genomic selection to evaluate growth and fat-related traits in Wenchang chickens.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycerol monolaurate attenuates necrotic enteritis in broilers by improving gut–liver health and remodeling the gut microbiota","authors":"Linglian Kong, Xinran Zhang, Xue Pan, Xuewei Dong, Zhigang Song","doi":"10.1186/s40104-026-01388-w","DOIUrl":"https://doi.org/10.1186/s40104-026-01388-w","url":null,"abstract":"Necrotic enteritis (NE) is a widespread avian intestinal disease that causes substantial economic losses in poultry production. With increasing restrictions on antibiotic use, sustainable alternatives are urgently needed. Glycerol monolaurate (GML) has anti-inflammatory and antimicrobial properties, but its role in preventing NE remains unclear. This study aimed to investigate the protective effects of GML on gut–liver health and the cecal microbiota in NE-challenged broilers.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"14 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147702314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline","authors":"Mingyue Gao, Junnan Zhang, Boxuan Zhang, Xinwei Jiang, Bowen Niu, Conghao Zhong, Fangren Lan, Wenxin Zhang, Ning Yang, Congjiao Sun","doi":"10.1186/s40104-026-01392-0","DOIUrl":"https://doi.org/10.1186/s40104-026-01392-0","url":null,"abstract":"Aging-related production decline is widely observed in poultry farming, making it challenging to prolong the laying cycle of hens. However, its genetic determinants remain unclear. This study aimed to systematically identify key genes associated with hens with age-related production decline and reveal dynamic changes in gene expression during tissue functional decline.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"31 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of gut microbiome and metabolomic profiles to POLYCAN, a β-glucan derived from Aureobasidium pullulans SM-2001 in beagles","authors":"Vetriselvi Sampath, Kyejin Lee, Minjeong Kim, Young Suk Kim, Dae Hong Min, Kyudong Han, Sungbo Cho, Dae-Kyung Kang, In Ho Kim","doi":"10.1186/s40104-026-01381-3","DOIUrl":"https://doi.org/10.1186/s40104-026-01381-3","url":null,"abstract":"The importance of glucan additives has been widely recognized in farm animals. Yet the precise role of POLYCAN, a β-glucan derived from the black yeast <italic>Aureobasidium pullulans</italic> SM-2001, remains limited in companion animals. Therefore, this study aims to evaluate its effect on performance, nutrient digestibility, hematology, and the gut microbiome and serum metabolites in beagle dogs.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"23 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147642132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histone lactylation maintains bovine early embryo development via regulating embryonic genome activation","authors":"Wenjie Yu, Yi Xia, Wenzhuo Wang, Zhiyuan Yang, Zhengqiu Liu, Xinqi Gan, Mengya Zhang, Beibei Zhou, Yunsheng Li, Yunhai Zhang, Zubing Cao","doi":"10.1186/s40104-026-01398-8","DOIUrl":"https://doi.org/10.1186/s40104-026-01398-8","url":null,"abstract":"Early embryo loss is an important factor affecting the reproductive capacity of cattle. Recent studies have revealed that during the process of embryonic genome activation (EGA), epigenetic modification, such as histone lactylation remodeling, is crucial for early embryonic development. However, the effects of histone lactylation on early embryonic development in bovines and the related mechanisms remain unknown. In this study, in vitro fertilized embryos were utilized to investigate the effects of histone lactylation on EGA and early embryo development in cattle.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"117 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite-mediated crosstalk: unraveling the interactions between gut microbiota and host in fatty liver hemorrhagic syndrome of laying hens","authors":"Shaobo Zhang, Xinghua Zhao, Xin He, Wanyu Shi, Ning Ma","doi":"10.1186/s40104-025-01319-1","DOIUrl":"https://doi.org/10.1186/s40104-025-01319-1","url":null,"abstract":"Fatty liver hemorrhagic syndrome (FLHS) in laying hens is a metabolic disorder characterized by excessive hepatic lipid accumulation, inflammation, and hemorrhage, bearing pathological similarities to human non-alcoholic fatty liver disease. With the rise of intensive poultry farming, the incidence of FLHS has markedly increased, resulting in significant economic losses in the poultry industry. The gut microbiota plays a crucial role in host digestion, metabolism, and immune regulation, particularly in liver diseases. Gut microbiota and its metabolites influence liver health via the gut-liver axis. This review aims to explore metabolite-mediated interactions between the laying hens and the gut microbiota, elucidating their role in the pathogenesis of FLHS. Host-derived metabolites, such as lipids, bile acids, amino acids, and carbohydrates, regulate the structure and function of the gut microbiota through the gut-liver axis, playing a role in FLHS progression. Concurrently, microbial metabolites, including short-chain fatty acids, bile acids, and amino acid derivatives, influence hepatic lipid metabolism, inflammation, and oxidative stress, driving the development of FLHS. Key microbes, such as <italic>Bacteroides</italic>, <italic>Lactobacillus</italic>, and <italic>Akkermansia muciniphila</italic>, are considered potential therapeutic targets due to their involvement in metabolite production. By integrating multi-omics data and mechanistic studies, this review highlights the central role of host–gut microbiota communication in FLHS and provides a theoretical basis and research direction for the development of microbiota-based intervention strategies.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"42 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145903755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring cattle structural variation in the era of long reads, pangenome graphs, and near-complete assemblies","authors":"George E. Liu","doi":"10.1186/s40104-025-01294-7","DOIUrl":"https://doi.org/10.1186/s40104-025-01294-7","url":null,"abstract":"Structural variations (SVs ≥ 50 bp) are a critical but underexplored source of genetic diversity in cattle, shaping traits vital for productivity, adaptability, and health. Advances in long-read sequencing, pangenome graph construction, and near-complete genome assemblies now allow accurate SV detection and genotyping. These innovations overcome the limitations of single-reference genomes, enabling the discovery of complex SVs, including nested and overlapping variants, and providing access to previously inaccessible genomic regions such as centromeres and telomeres. This review highlights the current landscape of cattle SV research, with emphasis on integrating long-read sequencing and pangenome frameworks to uncover breed-specific and population-level variation. While many SVs are linked to economically important traits such as feed efficiency and disease resistance, their broader regulatory impacts remain an active area of investigation. Emerging functional genomics approaches, including transcriptomics, epigenomics, and genome editing, will clarify how SVs influence gene regulation and phenotype. Looking forward, the integration of SV catalogs with multi-omics data, imputation resources, and artificial intelligence-driven models will be essential for translating discoveries into breeding and conservation applications. Integrating structural variants into breeding pipelines promises to revolutionize livestock genomics, enabling precision selection and sustainable agriculture despite challenges in cost, data sharing, and functional validation.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"86 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145583721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox status regulates eggshell color by modulating protoporphyrin IX biosynthesis via the SIRT1/PGC-1α/ALAS1 axis in brown-shelled hens","authors":"Yu Fu, Mingyuan Lu, Dongkai Liu, Jianping Wang, Haijun Zhang, Guanghai Qi, Jing Wang","doi":"10.1186/s40104-025-01292-9","DOIUrl":"https://doi.org/10.1186/s40104-025-01292-9","url":null,"abstract":"This study investigated the molecular mechanisms by which redox status regulates protoporphyrin IX (PpIX) biosynthesis and eggshell coloration in brown-shelled laying hens. This study consisted of two experiments involving 48 and 32 healthy 60-week-old Hy-Line Brown hens, respectively. The hens exhibited either dark (L* = 51.99 ± 2.08) or light (L* = 64.12 ± 3.02) brown eggshell colors. In Exp. 1, light brown-shelled hens were fed a basal diet (Lb group), while dark brown-shelled hens received either a basal diet (Db group) or a basal diet with 10 mg/kg ammonium metavanadate (Dbv group) for 20 d. In Exp. 2, light brown-shelled hens received either a basal diet (Lbc group) or a basal diet supplemented with 200 mg/kg resveratrol (Lbr group) for 12 weeks. Compared to the Db group, eggshell L* values increased, and PpIX concentrations in both eggshell and uterus decreased in Dbv and Lb groups. These groups also showed oxidative stress, as indicated by reduced hepatic T-SOD and CAT activities. Uterine redox status changes were further confirmed by increased T-AOC level (Dbv) and reduced CAT gene expression (Lb). These redox disturbances led to reduced expression of ND4 and COX1 mtDNA, decreased ATP production and CS activity, along with upregulation of IR, PI3K, HK, and PK gene expression, reflecting altered mitochondrial energy metabolism. Notably, the SIRT1/PGC-1α signaling cascade and its downstream target ALAS1 were significantly downregulated at both mRNA and protein levels in Dbv and Lb groups. Compared to the Lbc group, the Lbr group exhibited higher antioxidant capacity by increasing hepatic CAT activity and uterine T-SOD and GSH-Px activities, and reducing MDA levels. Moreover, the Lbr group restored mitochondrial function and PpIX biosynthesis by upregulating ND4 and COX1 mtDNA, CS and SDHA gene expression, and SIRT1/PGC-1α/ALAS1 signaling, while downregulating LDH activity and the expression of IR and PI3K, thereby alleviating eggshell color fading. Oxidative stress induces eggshell depigmentation by impairing mitochondrial function and downregulating the SIRT1/PGC-1α/ALAS1 pathway, leading to reduced PpIX biosynthesis. Specifically, vanadium-induced or endogenous oxidative stress disrupts mitochondrial energy metabolism and suppresses key components of this pathway, while resveratrol alleviates oxidative damage and restores mitochondrial function and ALAS1-driven PpIX synthesis through reactivation of the SIRT1/PGC-1α axis.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"25 1","pages":"157"},"PeriodicalIF":7.0,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145559482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mining expansin-like proteins from rumen microbiota and functional characterization of two anaerobic fungal expansin-like proteins","authors":"Hongjian Dai, Jian Gao, Yuling Wei, Qi Wang, Weiyun Zhu, Yanfen Cheng","doi":"10.1186/s40104-025-01287-6","DOIUrl":"https://doi.org/10.1186/s40104-025-01287-6","url":null,"abstract":"Sustainable livestock production is essential for food security and environmental management. Lignocellulosic biomass can be used in animal feed, thereby reducing feed production costs and enhancing sustainability. Expansin-like proteins (ELPs) play essential roles in plant cell wall degradation, yet their functions remain largely underexplored in rumen microbes. The purpose of this study was to investigate the effects of rumen microbial ELPs on lignocellulose degradation. This study systematically identified 396 ELPs within the rumen microbiota, uncovering remarkable diversity, particularly among anaerobic fungi. Three representative ELPs from Pecoramyces ruminantium F1 (PFLoos_1, PFSWO1_1, PFSWO2_1) were selected for biochemical characterization. While PFSWO2_1 could not be expressed, PFLoos_1 and PFSWO1_1 exhibited significant synergy with cellulases. The CBM10-containing PFSWO1_1 demonstrated superior thermal stability (up to 65 °C) and substrate affinity, increasing rice straw hydrolysis efficiency by 21.6% (reducing sugar yield) compared to cellulase alone. Structural analyses revealed that CBM10 enabled PFSWO1_1 to preferentially bind complex substrates, whereas the single-domain PFLoos_1 targeted simpler substrates. Notably, ELP pretreatment of corn stover significantly improved fermentation quality (pH and lactic acid) and nutritional value (neutral detergent fiber, acid detergent fiber, and water-soluble carbohydrates). These findings indicate that ELPs are abundant in the rumen and play a synergistic role in lignocellulosic biomass conversion.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"238 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145554481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}