Journal of Animal Science and Technology最新文献

{"title":"Rapamycin Treatment During Prolonged In Vitro Maturation Enhances the Developmental Competence of Immature Porcine Oocytes","authors":"Seung-Eun Lee, Han-Bi Lee, Jae-Wook Yoon, Hyo-Jin Park, So-Hee Kim, Dong-Hun Han, Eun-Seo Lim, Eun-Young Kim, Se-Pill Park","doi":"10.5187/jast.2023.e101","DOIUrl":"https://doi.org/10.5187/jast.2023.e101","url":null,"abstract":"Porcine oocytes undergo <italic>in vitro</italic> maturation (IVM) for 42–44 h. During this period, most oocytes proceed to metaphase and then to pro-metaphase if the nucleus has sufficiently matured. Forty-four hours is sufficient for oocyte nuclear maturation but not for full maturation of the oocyte cytoplasm. This study investigated the influences of extension of the IVM duration with rapamycin treatment on molecular maturation factors. The phospho-p44/42 mitogen-activated protein kinase (MAPK) level was enhanced in comparison with the total p44/42 MAPK level after 52 h of IVM. Oocytes were treated with and without 10 μM rapamycin (10 R and 0 R, respectively) and examined after 52 h of IVM, whereas control oocytes were examined after 44 h of IVM. Phospho-p44/42 MAPK activity was upregulated the 10 R and 0 R oocytes than in control oocytes. The expression levels of maternal genes were highest in 10 R oocytes and were higher in 0 R oocytes than in control oocytes. Reactive oxygen species (ROS) activity was dramatically increased in 0 R oocytes but was similar in 10 R and control oocytes. The 10 R group exhibited an increased embryo development rate, a higher total cell number per blastocyst, and decreased DNA fragmentation. The mRNA level of development-related (<italic>POU5F1 </italic>and<italic> NANOG) </italic>mRNA<italic>, </italic>oocyte-apoptotic (<italic>BCL2L1) </italic>genes were highest in 10 R blastocysts. These results suggest that prolonged IVM duration with rapamycin treatment represses ROS production and increases expression of molecular maturation factors. Therefore, this is a good strategy to enhance the developmental capacity in porcine oocytes.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135866357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of genomic regions and genes associated with subclinical ketosis in periparturient dairy cows","authors":"Jihwan Lee, KwangHyeon Cho, Kent Weigel, Heather White, ChangHee Do, Inchul Choi","doi":"10.5187/jast.2023.e97","DOIUrl":"https://doi.org/10.5187/jast.2023.e97","url":null,"abstract":"Subclinical ketosis (SCK) is a prevalent metabolic disorder that occurs during the transition to lactation period. It is defined as a high blood concentration of ketone bodies (beta-hydroxybutyric acid ≥ 1.2 mmol/L) within the first few weeks of lactation, and often presents without clinical signs. SCK is mainly caused by negative energy balance (NEB). The objective of this study is to identify single nucleotide polymorphisms (SNPs) associated with SCK using genome-wide association studies (GWAS), and to predict the biological functions of proximal genes using gene-set enrichment analysis (GSEA). Blood samples were collected from 112 Holstein cows between 5 and 18 days postpartum to determine the incidence of SCK. Genomic DNA extracted from both SCK and healthy cows was examined using the Illumina Bovine SNP50K BeadChip for genotyping. GWAS revealed 194 putative SNPs and 163 genes associated with those SNPs. Additionally, GSEA showed that the genes retrieved by DAVID (Database for Annotation, Visualization, and Integrated Discovery) belonged to calcium signaling, starch and sucrose, immune network, and metabolic pathways. Furthermore, the proximal genes were found to be related to germ cell and early embryo development. In summary, this study proposes several feasible SNPs and genes associated with SCK through GWAS and GSEA. These candidates can be utilized in selective breeding programs to reduce the genetic risk for SCK and subfertility in high-performance dairy cows.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135110586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A chrono-physiological management protocol in form of simultaneous shifting of both lighting-cycle and feeding-time can enhance the production performance of heat-stressed goat kids","authors":"Mohammed Al-Badwi, Emad Samara, Majdi Bahadi, Khalid Abdoun, Ayman Swelum, Abdullah Al-Owaimer, Ahmed Al-Haidary","doi":"10.5187/jast.2023.e99","DOIUrl":"https://doi.org/10.5187/jast.2023.e99","url":null,"abstract":"In a journey exploring the influence of two external zeitgebers and their interaction on the biophysiological and productive performance of goat kids exposed to heat stress, 15 healthy Aardi male kids (6 months of age and 22.56±1.13 kg) individually housed in climatic chambers were allocated into three groups (5 kids/group). Kids in the first group (the control group, C) were placed under a normal light:dark (12L:12D) cycle and fed in the morning. Kids in the second group (T1) were fed in the morning but placed under a reversed 12D:12L cycle. Kids in the third group (T2) were placed under a reversed 12D:12L cycle and fed in the evening. During the experimental period (~5 weeks), kids were exposed to a hot condition (as manifest by the temperature-humidity index) using a biometeorologically simulated environment with a daily ambient temperature cycle of 25°C to 45°C, and multiple data (i.e., meteorology, biophysiology, and performance) were obtained. Reversing the lighting cycle alone (T1) and/or the simultaneous shifting of both the lighting cycle and feeding time protocol (T2) under hot climatic conditions had no influence on body rectal and skin temperatures as well as plasma concentrations of albumin and glucose. Kids in both treatments showed (<italic>p</italic> <italic>&amp;lt;</italic> 0.05) higher coat temperature and respiratory rate as well as plasma concentrations of triacylglycerol compared to the C group kids. Moreover, it was clearly evident that kids in T2 had (<italic>p</italic> <italic>&amp;lt;</italic> 0.05) reduced kids DFI, increased (<italic>p</italic> <italic>&amp;lt;</italic> 0.05) their ADG, which subsequently had been reflected on having (<italic>p</italic> <italic>&amp;lt;</italic> 0.05) better FCR compared to kids in other groups. Collectively, this would suggest that using such chrono-physiological management protocol had desynchronized the heat load emerging from the combined effects of both thermal stress and post-prandial metabolism. Compared to other protocols, our findings point out that simultaneous shifting of both lighting cycle and feeding time protocol might be suitable in enhancing the production performance of growing heat-stressed goats.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"185 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135110587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex ratio and conception rates of fresh/vitrified embryos at different developmental stages by ovum pick up in Hanwoo cows","authors":"Jihyun Park, Seonggyu Bang, Wonyou Lee, Kilyoung Song, Miyun Park, Junseo Chung, Islam Saadeldin, Sanghoon Lee, Junkoo Yi, Jongki Cho","doi":"10.5187/jast.2023.e98","DOIUrl":"https://doi.org/10.5187/jast.2023.e98","url":null,"abstract":"Embryo transfer plays a crucial role in enhancing the breeding value of livestock; it has been applied in Hanwoo cattle, which is a popular breed for beef production in Korea. Both <italic>in vivo</italic>-derived (IVD) and <italic>in vitro</italic>-produced (IVP) embryos are used for this purpose; however, IVP embryos have been preferred recently owing to advancements in ovum pick-up (OPU) technology and genomic selection. Despite technological advancements, comprehensive data on large-scale OPU/IVEP/embryo transfer in Hanwoo cows are lacking. In this study, 16 elite Hanwoo donor cows were selected on the basis of specific criteria. Oocytes were retrieved from 241 cows using OPU. The collected cumulus-oocyte complexes (COCs) were matured, fertilized, and cultured <italic>in vitro</italic> to produce transferable embryos. Embryos were classified according to their developmental stage and then transferred to 675 recipient cows. A total of 3,317 COCs were collected, with an average of 13.76 COCs per cow. The number of transferable embryos produced per cow was 3.7. Hanwoo OPU-derived IVP embryos exhibited a higher production yield than the global average, indicating a stable IVEP environment. Both fresh and frozen IVP embryos yielded similar conception rates; hence, the use of vitrified-thawed embryos in transfer plans feasible. However, frozen-thawed embryos at Stage 7 had a lower conception rate than those at earlier stages. There was no significant difference between the conception rates of sexually mature heifers and postpartum cows used as recipients. The male-to-female offspring ratio increased as the developmental stage progressed. Seasonal effects on conception rates were not observed; however, higher abortion rates and a higher proportion of male offspring were observed during winter. This study provides valuable data for improving the Korean embryo transfer industry, enabling more strategic growth of the domestic Hanwoo cow production.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135110585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of planting soil temperature and GDD impacts on silage corn (Zea mays L.) biomass","authors":"Moonju Kim, Jiyung Kim, Mu-Hwan Jo, Kyungil Sung, Kun-Jun Han","doi":"10.5187/jast.2023.e100","DOIUrl":"https://doi.org/10.5187/jast.2023.e100","url":null,"abstract":"The annual forage crop production system, enclosing silage corn (<italic>Zea mays</italic> L.) and following cool-season annual forage, can enhance forage production efficiency where available land is limited for pasture production. In this forage production system, successful silage corn cultivation has a significant value due to the great yield of highly digestible forage. However, some untimely planting or harvesting of corn due to changing weather often reduces biomass and feeding values. Therefore, a study was conducted to quantify the corn silage biomass reductions by the deviations from optimum planting soil temperature and optimum growing degree day. The approximations of maximum corn production were estimated based on field trial data conducted between 1978 and 2018 with early, medium, and late-maturity corn groups. Based on weather data, the recorded planting dates and harvest dates were converted into the corresponding trials’ soil temperatures at planting (STP) and the growing degree days (GDD). The silage corn biomass data were regressed against STP and GDD using a quadratic function. The maximum biomass point was modeled in a convex upward quadratic yield curve and the optimum STP and GDD were defined as those values at the maximum biomass for each maturity group. Optimized STP was at 16.6, 16.2, and 15.6°C for early, medium, and late maturity corn groups, respectively, while optimized GDD at harvest was at 1424, 1363, and 1542 °C. The biomass reductions demonstrated quadratic functions by the departures of STP or GDD. The 5% reductions were anticipated when STP departed from the optimum temperature by 2.2, 2.4, and 1.4°C for early, medium, and late maturity corns, respectively; the same degree of reductions were estimated when the GDD departed by 200, 180, and 130°C in the same order of the maturity groups. This result indicates that biomass reductions of late-maturity corn were more sensitive to the departures of STP or GDD than the early-maturity corn. Therefore, early maturing cultivars are more stable in biomass production in a silage corn–winter annual forage crop production system to enhance forage-based livestock production efficiency.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135110589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of supplemental bacteriophage on the gut microbiota and nutrient digestibility of ileal-cannulated pigs","authors":"Hyunwoong Jo, Geongoo Han, Eun Bae Kim, Changsu Kong, Beob Gyun Kim","doi":"10.5187/jast.2023.e96","DOIUrl":"https://doi.org/10.5187/jast.2023.e96","url":null,"abstract":"This study measured the potential changes of the microbiota in the gastrointestinal tract and energy and nutrient digestibility by supplemental bacteriophages in pigs. Twelve castrated male pigs (initial mean body weight = 29.5 ± 2.3 kg) were surgically cannulated using T-cannula. The animals were housed individually in pens equipped with a feeder and a nipple waterer. The pigs were allotted to 1 of 3 experimental diets in a quadruplicated 3 × 2 Latin square design with 3 experimental diets, 2 periods, and 12 pigs resulting in 8 replicates per diet. The 3 diets were a control mainly based on corn and soybean meal with no antibiotics or bacteriophages, a diet containing 0.1% antibiotics, and a diet containing 0.2% bacteriophages. On day 5 of the experimental period, feces were collected and on days 6 and 7, ileal digesta were collected. Genomic DNA for bacteria were extracted from the ileal digesta and feces and the V4 region of the 16S rRNA gene was amplified. The ileal and fecal digestibility of energy, dry matter, organic matter, crude protein, and fiber was unaffected by dietary antibiotics or bacteriophages. At the phylum level, the supplemental antibiotic or bacteriophage tended to result in a higher proportion of <italic>Firmicutes</italic> (<italic>p</italic> = 0.059) and a lower proportion of <italic>Bacteroidetes</italic> (<italic>p</italic> = 0.099) in the ileal digesta samples compared with the control group with no difference between the antibiotic and bacteriophage groups. At the genus level, the supplemental antibiotic or bacteriophage tended to result in a higher proportion of <italic>Lactobacillus</italic> (<italic>p</italic> = 0.062) and a lower proportion of <italic>Bacteroides</italic> (<italic>p</italic> = 0.074) and <italic>Streptococcus</italic> (<italic>p</italic> = 0.088) in the ileal digesta compared with the control group with no difference between the antibiotic and bacteriophage groups. In the feces, supplemental antibiotics or bacteriophages reduced the proportion of <italic>Bifidobacterium</italic> compared with the control group (<italic>p</italic> = 0.029) with no difference between the antibiotic and bacteriophage groups. Overall, supplemental antibiotics and bacteriophages showed positive effect on the microbiota of in the ileal digesta without largely affecting energy or nutrient digestibility, with no differences between the antibiotic and bacteriophage groups in growing pigs.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"365 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136023137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of paraformic acid supplementation, as an antibiotic replacement, on growth performance, intestinal morphology and gut microbiota of nursery pigs","authors":"Yuyi Zhong, Bin Zuo, Jiaqi Li, Yafeng Zhai, Richard Mudarra","doi":"10.5187/jast.2023.e95","DOIUrl":"https://doi.org/10.5187/jast.2023.e95","url":null,"abstract":"49 A total of 150 crossbred male pigs [21±1 days old; 8.85±0.15 Kg body weight (BW)] were randomly assigned to five 50 dietary treatments with five replicates per treatment and six pigs per pen to evaluate the effect of paraformic acid 51 (PFA), as a substitute to antibiotics, on growth performance, intestinal morphology, and gut microbiota of nursery 52 pigs. The treatments were: 1) NC: nutrient adequate control diet; 2) PFA1: similar to NC plus 0.30% PFA; 3) PFA2: 53 similar to NC plus 0.60% PFA; 4) PFA3: similar to NC plus 1.0% PFA; and 5) PC: similar to NC plus 0.15% of 54 chlortetracycline. Pigs were fed the same nutritional profile during the two-phase feeding regime [phase 1 (P1; d 0– 55 14), and phase 2 (P2; d 15–30)]. Initial BW, and BW and feed disappearance at the end of each phase were recorded 56 to calculate average daily feed intake (ADFI), average daily gain (ADG), and feed to gain ratio (F: G). The Fecal score 57 was determined at the end of P1, while the intestinal morphology and microbiota analysis were performed at the end 58 of P2. Pigs fed PFA2 had higher ADG than those fed NC in P1. A quadratic response was found in the overall phase 59 1 and phase 2 (P1&2) with the highest ADG in pigs fed PFA2 ( p < 0.05). Pigs fed PC had the highest ADFI during 60 P2 and overall P1&2 ( p < 0.05). The PFA2 group had the lowest F:G ratio among treatments in P1 and P2, with a 61 quadratic response in the overall P1&2 ( p < 0.05). Pigs fed PFA1, PFA2, PFA3, and PC showed better fecal 62 consistency than NC ( p < 0.05). No differences were found in intestinal morphology among treatments. PFA groups 63 supplementation modulated the relative abundance of Lactobacillus and Streptococcus in the jejunum. In the cecum, 64 PFA2 had a higher relative abundance of Prevotella when compared to NC, but lower than PC. In addition, pigs fed 65 the NC diet had higher abundance of Treponema and Methanobrevibacter than other treatments. In conclusion, the 66 supplementation of 0.6% PFA improved growth performance and modulated gut microbiota in nursery pigs. 67","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135097011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lauric acid reduces apoptosis by inhibiting FOXO3a-signaling in Deoxynivalenol-treated IPEC-J2 cells","authors":"Na Yeon Kim, Sang In Lee","doi":"10.5187/jast.2023.e92","DOIUrl":"https://doi.org/10.5187/jast.2023.e92","url":null,"abstract":"Deoxynivalenol (DON) is the most common mycotoxin contaminant of food or feed worldwide and causes disease in animals. Lauric acid (LA) is a medium-chain fatty acid useful for barrier functions such as antimicrobial activity in the intestine of monogastric animals. However, the molecular mechanisms by which lauric acid exerts its effects on the deoxynivalenol-exposed small intestine have not been studied. We used an intestinal porcine epithelial cell line (IPEC-J2) as an <italic>in vitro</italic> model to explore the molecular mechanism of lauric acid in alleviating deoxynivalenol-induced damage. We found that lauric acid reversed deoxynivalenol-induced reduction in cell viability. Our qRT-PCR results indicated that lauric acid alleviated deoxynivalenol-induced apoptosis through Annexin-V. Additionally, immunofluorescence and Western blotting showed that lauric acid attenuated deoxynivalenol-induced Forkhead box O3 (FOXO3a) translocation. These results suggest that lauric acid attenuates Forkhead box O3 translocation in the small intestine damaged by deoxynivalenol, thereby reducing apoptosis. In conclusion, this study found that lauric acid alleviates deoxynivalenol-induced damage in intestinal porcine epithelial cell line through various molecular mechanisms.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135453166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of zinc oxide and copper oxide nanoparticles as potential alternatives to antibiotics for managing fowl typhoid in broilers","authors":"Muhammad Raza, Kim Eungyung, Muhammad Shakeel, Muhammad Fiaz, Lei Ma, Hyeonjin Kim, Chae Yeon Kim, Dongwook Kim, Kanghyun Park, Muhammad Tariq Javed, Myoungok Kim","doi":"10.5187/jast.2023.e91","DOIUrl":"https://doi.org/10.5187/jast.2023.e91","url":null,"abstract":"","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"159 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135453760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dairy based-LAB Improved Constipation Via Increasing Fecal Bulk and Decreasing Concentration of Fecal Threonine While Preserving Colonic Goblet Cell Count","authors":"Mohamad Jaafar, Pei Xu, Uma-Mageswaran Mageswaran, Shandra-Devi Balasubramaniam, Maheswaran Solayappan, Jia Woon, Cindy Teh, Svetoslav Todorov, Yong Park, Guoxia Liu, Min Liong","doi":"10.5187/jast.2023.e93","DOIUrl":"https://doi.org/10.5187/jast.2023.e93","url":null,"abstract":"","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135453012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}