Accomplishment of canine cloning through in vitro matured oocytes: a pioneering milestone.

IF 2.7 3区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Animal Science and Technology Pub Date : 2024-05-01 Epub Date: 2024-05-31 DOI:10.5187/jast.2024.e18
Kukbin Ji, Kangsun Park, Dongern Kim, Eunyoung Kim, Taeyoung Kil, Minkyu Kim
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Abstract

The in vitro maturation (IVM) rate of canine oocytes remains low compared to other mammals due to their unique reproductive characteristics. This study aimed to explore the effect of hormone supplementation during the IVM of canine immature oocytes on nuclear maturation and subsequently assess its potential application in canine somatic cell nuclear transfer (SCNT). Immature oocytes were collected and cultured in an IVM medium supplemented with hormones (follicle-stimulating hormone [FSH] and progesterone [P4]) or without hormones (control) for 24 hours. The maturation rates of oocytes in the hormone-treated group (94.92 ± 3.15%) were significantly higher than those in the control group (61.01 ± 4.23%). Both in vitro and in vivo matured oocytes underwent NT to evaluate their utility, and the fusion rates were higher in the in vitro matured group than those in the vivo matured group, not significant between in vivo and in vitro matured group (73.28% and 82.35%, respectively). As a result, 14 fused embryos from the in vitro matured group were transferred into two surrogates, with one surrogate achieving a successful pregnancy and delivering four puppies. Whereas in the in vivo matured group, 85 fused embryos were transferred to 8 surrogate mothers, leading to three surrogates becoming pregnant and delivering one, four, and two puppies. The pregnancy rates were not significant between both groups (50% and 37.50%), but the number of offspring exhibited a significant difference (28.57% and 8.23%). In conclusion, we achieved a remarkable milestone by successfully producing cloned puppies using in vitro matured oocytes, underscoring the feasibility of canine cloning from in vitro recovered oocytes. It is important to note that this study focused only on immature oocytes after ovulation and only during the estrus stage. Further research targeting other stages of the estrous cycle could potentially enhance canine cloning efficiency.

通过体外成熟卵母细胞完成犬克隆:一个开创性的里程碑。
与其他哺乳动物相比,犬卵母细胞的体外成熟(IVM)率仍然很低,这是因为犬具有独特的生殖特性。本研究旨在探索犬未成熟卵母细胞体外成熟过程中补充激素对核成熟的影响,并评估其在犬体细胞核移植(SCNT)中的潜在应用。收集未成熟卵母细胞并在添加激素(卵泡刺激素 [FSH] 和黄体酮 [P4])或不添加激素(对照组)的 IVM 培养基中培养 24 小时。激素处理组的卵母细胞成熟率(94.92 ± 3.15%)明显高于对照组(61.01 ± 4.23%)。体外和体内成熟的卵母细胞都进行了NT以评估其效用,体外成熟组的融合率高于体内成熟组,但体内成熟组和体外成熟组的融合率差异不显著(分别为73.28%和82.35%)。结果,体外成熟组的 14 个融合胚胎被移植到两个代孕动物体内,其中一个代孕动物成功怀孕并产下 4 只幼犬。而体内成熟组有 85 个融合胚胎移植给了 8 位代孕母亲,结果有 3 位代孕母亲怀孕,并分别产下 1 只、4 只和 2 只小狗。两组的妊娠率差异不大(50% 和 37.50%),但后代数量差异显著(28.57% 和 8.23%)。总之,我们利用体外成熟卵母细胞成功培育出克隆幼犬,这是一个了不起的里程碑,凸显了利用体外回收卵母细胞克隆犬类的可行性。值得注意的是,这项研究只关注排卵后和发情期的未成熟卵母细胞。针对发情周期其他阶段的进一步研究可能会提高犬克隆的效率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Animal Science and Technology
Journal of Animal Science and Technology Agricultural and Biological Sciences-Food Science
CiteScore
4.50
自引率
8.70%
发文量
96
审稿时长
7 weeks
期刊介绍: Journal of Animal Science and Technology (J. Anim. Sci. Technol. or JAST) is a peer-reviewed, open access journal publishing original research, review articles and notes in all fields of animal science. Topics covered by the journal include: genetics and breeding, physiology, nutrition of monogastric animals, nutrition of ruminants, animal products (milk, meat, eggs and their by-products) and their processing, grasslands and roughages, livestock environment, animal biotechnology, animal behavior and welfare. Articles generally report research involving beef cattle, dairy cattle, pigs, companion animals, goats, horses, and sheep. However, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will also be considered for publication. The Journal of Animal Science and Technology (J. Anim. Technol. or JAST) has been the official journal of The Korean Society of Animal Science and Technology (KSAST) since 2000, formerly known as The Korean Journal of Animal Sciences (launched in 1956).
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