Journal of Advances in Microbiology最新文献

{"title":"In-silico Analysis of Newcastle Disease Virus Strains from Outbreaks in Zaria and Kano of Nigeria","authors":"Mohammed Usman Sajo, Bello Usman, Musa Kalim Adam, Mubarak Sambo, Maman Moutari Souley, Olusegun Adesina Fagbohun, Lawal Sa’idu","doi":"10.9734/jamb/2023/v23i10759","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i10759","url":null,"abstract":"Introduction: Newcastle Disease (ND) is the major constraint to poultry production in developing country like Nigeria. Numerous outbreak cases of ND have being reported frequently even in vaccinated farms. The large antigenic disparity of the Newcastle Disease Virus (NDV) and potential for significant evolutionary divergence between field strains and widely used vaccinations are thought to be the root of the issue.
 Aim/Objectives: A cross-sectional studies was carried-out to identify a discrepancy between the vaccinal strains and circulating NDV field strains in Zaria and Kano so as to provide solution for the control of ND.
 Materials and Methods: In this study, molecular characterization and pathotyping of the field strains of NDV and strains of ND vaccines was carried out to evaluate their relatedness. Using RT-PCR with primers targeting partial fusion gene, 19 out of the (n= 120) samples purposefully collected for the detection of NDV in Zaria (n= 44) and Kano (n= 76) were positives. Twelve of the 19 positive amplicons were successfully sequenced and analyzed.
 Results: Only few number of the study’s sample have similarity with some of the vaccine strains.
 Conclusion: This study has raised concerns about the methods used to prevent NDV in Nigeria.
 Recommendation: It is crucial to develop an ND vaccine that matches the field strains within the country, along with ongoing surveillance of outbreaks for the effective control of the disease.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136342347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety Concerns on Microbes Associated with Fresh and Smoked Fish Sold in Igbokoda Fish Market, Nigeria","authors":"Olagunju Johnson Adetuwo, Kehinde Tope Adegbehingbe, Omodara Tolani Rachael","doi":"10.9734/jamb/2023/v23i10758","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i10758","url":null,"abstract":"Fishes are important source of food for human globally because man gets a lot of minerals, vitamins, lipids and proteins from fishes and theirs products. Microbiological quality of fish is important to public health as indicative parameters to ensure safe consumption of fish by man. This study was carried out in order to evaluate microbes associated with fresh and smoked fishes on sales at Igbokoda fish market and their antimicrobial susceptibility profiles. Ten different fresh and smoked fish samples were collected from different fish-sellers at different selling points in Igbokoda fish market, aseptically using sterile containers. Samples from the skin, intestine and gill were obtained and cultured into three different media (MacConkey Agar, Nutrient Agar, Salmonella-Shigella Agar) for bacteria isolation and characterization. Other samples from the skin, intestine and gill were also cultured into Potato Dextrose Agar for fungal isolation and characterization. After 48 hours, on examination the total bacterial counts obtained from the skin, intestine and gill ranged between 2.4 to 8.7 x 106 cfu/ml while the total fungal counts ranged between 4.6 to 9.2 x 104 cfu/ml. Highest microbial load was obtained from the skin of the fish samples (7.2 x 106 cfu/ml), while the gill had the lowest microbial load (4.6 x 104 cfu/ml). The bacteria species isolated from the fish samples and their percentage of occurrence were; Aeromonas hydrophilia (11.2%), Bacillus species (6.8%), Citrobacter freundii (3.3%), Escherichia coli (23.2%), Enterobacter cloacae (6.1%), Enterococcus faecalis (6.5%), Listeria monocytogenes (4.5%), Pseudomonas aeroginosa (8.2%), Salmonella species (10.2%), Staphylococcus aureus (16.5%) and Streptococcus species (3.5%). The fungi isolated and their percentage of occurrence were; Penicillium species (16.2%), Aspergillus flavus (8.4%), Aspergillus niger (25.5%), Candida species (16.4%), Fusarium species (6.5%), Mucor species (8.6%), Rhodotorula species (7.2%) and Rhizopus stolonifer (11.2%). Antibiotics susceptibility testing showed that all bacteria isolates were susceptible to Chloramphenicol, Ciprofloxacin, Augmentin, and Amoxillin. While Salmonella species, Staphylococcus aureus and Streptococcus species showed strong resistance to Septrin, Gentamycin and Streptomycin but intermediate susceptible to Erythromycin, Ampiclox, and Tetracycline. The antifungal susceptibility testing of the fungal isolated from fish samples showed that all the isolates were susceptible to all the antifungal agents used except Candida species that showed little resistance to clotrimazole. The study showed that microbial loads in the fish samples were low and, also there was no presence of multidrug resistant bacteria in sampled fish; therefore the fishes were safe for consumption. Although, it is recommended that good processing method should be adopted by fish handlers to prevent health risks to the fish consumers.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135199007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Virulence Genes in Extended Spectrum Beta Lactamase-Producing Escherichia coli Isolated from Fresh Leafy Vegetables Sold in Rivers State, Nigeria","authors":"Okwelle, Austin Achinike, None Osakuade, Felicia O.","doi":"10.9734/jamb/2023/v23i10757","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i10757","url":null,"abstract":"Extended-spectrum β-lactamases (ESBL’S) are a rapidly growing group of β-lactamases which have the ability to hydrolyze third generation antibiotics such as Cephalosporins and Aztreonam. The aim of this study is the determination of virulence genes in ESBL- producing Escherichia. coli isolated from cabbage, spinach and water leaf. but are inhibited by Clavulanic Acid. The randomised experimental design was used to collect cabbage (Brassica oleracea), water leaf (Hydrophyllum macrophyllum) and spinach (Spinacia oleracea) samples from retailer sellers in two different markets (Choba and Rumuosi daily markets) of Obio/Akpor Local Government Area, Rivers State. Water leaf samples had a mean count of 2.9×103, spinach sample mean count was 2.8×104 Cfu/g and the highest, while the lowest mean count was obtained from cabbage samples; 1.6×103 Cfu/g. The study has established the presence of pathogenic E. coli (Enterohaemorrhagic) which are Shiga toxin producers that are Highly virulent with low infections doses.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135014722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Lactobacillus Isolated from the Microflora of Different Fermentation Processes and Doderlein Flora","authors":"Coulibaly Adja Mansagna, Coulibaly Ibourahema, Ouattara Mohamed Baguy, Tiekoura Konan Bertin, Guédé Kipre Bertin, Toty Abale Anatole, Konan Kouadio Fernique, Mariko Safiatou Tenin. Soumahoro, Nathalie Kouadio Guessennd, Le GER-BMR","doi":"10.9734/jamb/2023/v23i10756","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i10756","url":null,"abstract":"Lactobacillus contribute to the organoleptic quality of foods during the fermentation process in which they are involved. They also have the ability to inhibit the growth of undesirable germs by producing metabolites such as bacteriocins. The aim of this study was to isolate and identify the different Lactobacillus species from various fermentation processes and from the Doderlein flora. Lactobacillus were isolated from raw cow milk, curdled milk, cassava ferment (Magnan) and Doderlein flora on MRS agar. Strains were identified by mass spectrometry (MALDI-TOF). 213 Lactobacillus strains were isolated and identified from the different matrices. The main species isolated from raw cow's milk and curdled milk were Lactobacillus plantarum (31.58%), Lactobacillus casei (26.31%), Lactococcus lactis (21.05%), Lactobacillus delbrueckii spp bulgaricus (31%), Lactobacillus helveticus (30%) and Lactobacillus fermentum (25%) respectively. In the cassava ferment, Lactobacillus plantarum (37.5%), Lactobacillus rhamnosus (25%) and Lactobacillus reuteri (25%) were the most isolated species. The main species of Doderlein flora were Lactobacillus acidophilus (50%) and Lactobacillus plantarum (25%). This study showed a diversity of Lactobacillus present and involved in the different fermentation processes of foods and Doderlein flora (Commensal flora of the vagina). The qualitative and quantitative proportions of the different Lactobacillus species depend on the matrix. These Lactobacillus can potentially produce substances (bacteriocins) that inhibit the growth of pathogenic bacteria and multi-resistant bacteria.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135153385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological Impact of Fermented Soybean Condiments Produced from Indigenous Fermenters","authors":"C. M. Iheukwumere, I. H. Iheukwumere, U. O. Okoli, C. H. Ugwu","doi":"10.9734/jamb/2023/v23i10754","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i10754","url":null,"abstract":"Several studies have shown that most condiments consumed in Nigeria today are fortified with chemicals that alter the nature and stripped the natural immunological adjuvants designed by nature to protect the body system. Several condiments have been developed from fermented foods in order to control this ugly situation but there is still controversial thought in the choice of microorganism to be used in order to produce a non-toxic condiment with an improved immunological function. The aim of this study was to evaluate the toxicity, nutritive value and immunological impact of fermented soybean condiments. Soybean (Glycine max) sample was fermented with indigenous microorganisms isolated from 7 days old fermented soybean sample; this was oven-dried, pulverized and packaged in a cleaned sterile screw capped container. The immunological impact, pro-inflammatory and anti-inflammatory activities were determined using in vivo and instrumentation techniques. Lactobacillus plantarum strain ZS 2058 (L), Bacillus subtilis strain 168 (B) and Saccharomyces cerevisiae strain YJM555 (Y) were the indigenous microbes used both singly and in consortium for the production of light to dark brown condiments with water activity ranging from 0.27 – 0.37 for the fermented soybean in the plate and 0.22 - 0.36 for the fermented soybean wrapped with Thaumatococcus danielli leaves (called Uma in Igbo and Ewe eran in Yoruba). There was pronounced delay type hypersensitivity (DTH) and significant (P<0.05) elevation of lymphocyte population, rosette counts, antibody titers, phagocytic indices, total lymphocytes and malondialdehyde (MDA) values, of which pronounced activities were mostly seen on those condiments prepared with consortium of the organisms (BLY). Therefore condiments produced from fermentation of soybean using indigenous B, L, Y, BL and BLY are recommended as this would enhance its immunological impact and also safe, and those fermented in plastic plates using BLY were most efficient, preferable and acceptable.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135436859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity of Prepared Fermented Soybean Condiments from Indigenious Fermenters","authors":"C. M. Iheukwumere, I. H. Iheukwumere, C. H. Ugwu, U. O. Okoli","doi":"10.9734/jamb/2023/v23i10755","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i10755","url":null,"abstract":"Several studies have shown that most condiments consumed in Nigeria today are fortified with chemicals that alter the nature, and negatively affect organs and cells. Several condiments have been developed from fermented foods in order to control this ugly situation and to produce a non-toxic condiment with improve health qualities. The aim of this study was to evaluate the toxicity of the fermented soybean condiments produced using indigenous fermenters. Soybean (Glycine max) sample was fermented with indigenous microorganisms isolated from 7 days old fermented soybean sample; this was oven-dried, pulverized and packaged in a cleaned sterile screw capped container. The toxicity was evaluated using in vivo technique. Lactobacillus plantarum strain ZS 2058 (L), Bacillus subtilis strain 168 (B) and Saccharomyces cerevisiae strain YJM555 (Y) were the indigenous microbes used singly and in consortium for the production of light to dark brown condiments with water activity ranging from 0.27 – 0.37 for the fermented soybean in the plate and 0.22 - 0.36 for the fermented soybean wrapped with Thaumatococcus danielli leaves (called Uma in Igbo and Ewe eran in Yoruba). No death was recorded from the experimental rats.The experimented rats showed significant (P<0.05) increased in body weight, no evidence of severe deformities in the organs and cells, slight or no increased in organ weights and these were statistically non-significant (P>0.05). Therefore condiments produced from fermentation of soybean using indigenous B, L, Y, BL and BLY are recommended safe and non-toxic and those fermented in plastic plates using BLY were most efficient, preferable and acceptable.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"205 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135397615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and Evaluation of IgM and NS1 Ag Detection for Early Diagnosis of Dengue Cases in a Tertiary Care Hospital","authors":"Charu Jain, Nikita Birhman, Swati Sinha, Anshdha Shah, Shukla Das, N. P. Singh, Poonam Mathur, Narendra Singh Mogha, Divya Kashyap","doi":"10.9734/jamb/2023/v23i10753","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i10753","url":null,"abstract":"Introduction: Dengue fever, a mosquito borne illness, have grown to pose a serious danger to public health systems in developing nations. The Dengue virus (DENV), a member of the Flavivirus genus has four serotypes that cause the disease which range in severity from subclinical infections to severe disease with high morbidity &amp; mortality. This study aims to assess the seroprevalence of dengue fever cases in a tertiary care hospital from East- Delhi region.&#x0D; Material &amp; Methods: This study was a retrospective observational study conducted in the Department of Microbiology at UCMS &amp; GTBH. The data was collected from laboratory records from a period of Jan 2019 to December 2019.&#x0D; Results: A total of 1449 clinically suspected cases of dengue were tested for the presence of NS1 antigen and IgM antibodies by capture ELISA. This study showed 32.22% (467/1449) seropositivity of dengue infection. Out of 1272, a total of 456 samples were positive for NS1 Ag ELISA. Whereas, out of 177, a total 11 samples were positive for IgM ELISA.&#x0D; Observation: The study highlights the importance of early diagnosis and prompt management of dengue cases to prevent severe disease and reduce morbidity and mortality. This underscores the need for increased awareness, early detection, and appropriate management of dengue fever in endemic areas. especially during the peak months of October and November.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134913484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological Evidence of Chikungunya Infection in Kaltungo, Gombe State, Nigeria","authors":"Bashir Muhammad, Momodu Idris Babatunde, Umar Adamu Musa, Abdullahi Tanimu Bindawa, Jennifer Johnny, Mu’azzam Hassan Aliyu, Sufyan Chiroma Salihu, Ayuba Isiyaku, Katimu Yusuf, Musa Sale Makeri","doi":"10.9734/jamb/2023/v23i10752","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i10752","url":null,"abstract":"Introduction: Chikungunya is a re-emerging arthropod-borne viral disease that displays a large cell organ tropism, and causes a broad range of clinical symptoms in humans. The virus is listed on the WHO blueprint priority pathogens. However, Chikungunya is a neglected tropical disease in Nigeria and has never been investigated in Kaltungo. Chikungunya virus resembles malaria/typhoid fever in clinical syndrome; misdiagnosis is often common among clinicians.&#x0D; Aim/Objectives: A descriptive cross-sectional hospital-based study was carried out aimed at “serological evidence of acute chikungunya virus infection among outpatients with febrile illness attending general hospital Kaltungo, Gombe State Nigeria”.&#x0D; Materials and Methods: Rapid Test Device (RTD) and enzyme-linked immunosorbent assay (ELISA) were used to demonstrate the presence of acute infection due to CHIKV. A well-structured pre-tested questionnaire along with the consent forms was used to collect both demographic and clinical information of the study participants.&#x0D; Results: Sera of 200 consented patients examined, 112(56%) females and 88(44%) males were collected. Chi-square test was used for the analysis. Of the Sera tested, 14(7.0%) and 18(9.0%) were found to be positive for CHIKV IgM antibody using RTD and ELISA, respectively. There were statistical significant associations between gender, age and CHIKV infection (P≤0.05); males had 9(4.5%) IgM while females had 5(2.5%), age group 31-40 years had the highest positivity of 3.5% IgM and was found to be more infected with the virus. IgM antibody was detected higher 16(8.0%) among samples collected from 1-7 days while only 2(1.0%) were obtained on samples collected from 7-10 days. Antimalarial/antibiotic intake and recent travelling had not shown any statistical significant association (P&gt;0.05).&#x0D; Conclusion: The techniques used in this study revealed the presence of CHIKV IgM antibody among febrile patients evidencing the existence of CHIKV in Kaltungo.&#x0D; Recommendation: There is need for the inclusion of CHIKV in routine differential diagnosis of febrile patients to avoid misdiagnosis of febrile conditions.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135878891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical and Microbiological Assessment of Utaewa Estuary Water","authors":"Jonathan Okokon Ekanem, A. Y. Itah, Uduak Ndubuisi-Nnaji, Patience Uche-awaji Gabriel, Mkpoikanke Ibanga Raphael, Mary Ufon Godswill","doi":"10.9734/jamb/2023/v23i10751","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i10751","url":null,"abstract":"Most communities in the oil rich Niger Delta find it difficult to access to potable water due to constant pollution of their natural water sources. The sole aim of this study was to examine the microbiological and physicochemical qualities of the Estuary water at Utaewa to ascertain its suitability for potable use. Standard culture dependent techniques as well as metagenomics approach using Next Generation Sequencing (NGS) on Illumina Miseq platform was used to determine the microbiological characteristics while standard analytical procedures were employed to ascertain the physicochemical properties of Utaewa in Imo River. The average microbial counts ranged as follows: Total Heterotrophic Bacteria; 2.17±0.08x106 to 2.23±0.08x106 cfu/ml, Hydrocarbon Utilizing Bacteria; 1.03±0.06x105 to 1.39±0.08x105 cfu/ml, Total Heterotrophic Fungi; 1.23±0.13x105 to 1.64±0.09x105 cfu/ml, Hydrocarbon Utilizing Fungi; 7.9±0.21x103 to 8.6±1.00x103 cfu/ml. Bacterial isolates belonging to the genera Escherichia, Citrobacter, Bacillus, Salmonella, Shigella, Proteus, Flavobacterium, Vibrio, Micrococcus and Pseudomonas. were isolated and identified. The fungal isolates belonged to the genera Aspergillus, Rhizopus, Fusarium, Penicillium, Saccharomyces and Candida. On the other hand, Unknown (50.16%), Acinetobacter (8.34%), Ignatzschineria (4.73%), Planctomyces (3.28%), Anaerospora (3.27%), RS62 (2.73%), Methylophaga (1.64%), Wohlfahrtiimonas (1.31%), Myroides (1.23%) and Candidatus (0.95%) were captured by metagenomics analysis at the generic level. All the physicochemical parameters conformed to WHO and NIS standards for potable water except for Turbidity and Magnesium. Cultural methods used in this study were able to identify many potential water borne pathogens, metagenomics captured more microbial groups and give a better insight to bacterial composition and diversity. The presence of theses pathogenic bacteria underlies poor water quality and can pose public health threat to man and aquatic fauna. There is need to adhere to good hygienic practices and minimize the direct discharge of waste without proper treatment.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"273 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72917617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Some Fermentation Conditions for Bioethanol Production from Yam Peels (Dioscorea rotundata) Using Box-Behnken Design (BBD)","authors":"Z. Saulawa, L. Nura, Muntari Bala, Abdullahi Abdulkadir Imam","doi":"10.9734/jamb/2023/v23i9750","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i9750","url":null,"abstract":"Aim: The aim of this research was to optimize the production of bioethanol from a select second generation feedstock (yam peels). \u0000Methodology: Bioethanol was produced from yam peels using Box Behnken Design (BBD). The independent variables selected for optimization of bioethanol yield were; fermentation temperature (˚C), fermentation time (hours) and yeast concentration (%w/v). A set of 17 experiments were considered by design expert software. All experiments were run in triplicates. Test for bioethanol was carried out using acidified potassium dichromate method and absorbance was taken using a spectrophotometer at 600nm. \u0000Results: From the experiments carried out above, the optimum conditions under which the highest bioethanol (yield%) was produced were a fermentation temperature of 30̊C, yeast concentration of 5.50%w/v and fermentation time of 96hours under which a bioethanol yield of 45.79% was produced. \u0000Conclusion: Yam peels may therefore have the potentials to serve as substrates for bioethanol production rather than using food crops such as sugarcane juice and cassava flesh which may lead to food shortages and crisis especially is developing countries such as Nigeria.  ","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82686029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}