Journal of Advanced Pharmaceutical Technology & Research最新文献

{"title":"Exploring the bioactive compounds and molecular mechanisms of onion (<i>Allium cepa</i> L.) peels against prostate cancer through molecular docking and network pharmacology.","authors":"Paulus Chandra, Santoso Chandra","doi":"10.4103/JAPTR.JAPTR_232_25","DOIUrl":"10.4103/JAPTR.JAPTR_232_25","url":null,"abstract":"<p><p>Studies have shown onion peels to have potential anticancer effects on prostate cancer (PCa) development but with limited understanding of the underlying mechanisms. Therefore, this study aimed to explore potential bioactive compounds, molecular mechanisms, target proteins, and synergistic interactions of onion peels against PCa development. This study employed literature searching and pharmacokinetic property prediction to identify candidate compounds in onion peels. Swiss Target Prediction and Similarity Ensemble Approach were used to screen for potential compounds' targets, while GeneCards was searched for PCa related targets. Ten candidate compounds were identified, and target screening yielded 117 intersecting targets, used to construct protein-protein interaction (PPI) networks. Topological parameter analyses identified 21 core targets, while computationally enriched pathways include endocrine resistance, HIF 1 and PI3K-AKT signaling pathway, PCa, and pathways in cancer. Molecular docking was performed for all candidate compounds paired with each core target and revealed RAC alpha serine/threonine protein kinase 1 as the primary target, involved in all five highest affinity pairs: Quercetin, morin, isorhamnetin, kaempferol, and epicatechin. Potential essential amino acid residues included leucine 264, tryptophan 80, lysine 268, valine 270, threonine 211, and leucine 210. In conclusion, these findings provide computational evidence supporting the predicted underlying mechanisms, bioactive compounds, and targets potentially associated with the anticancer effects of onion peels in PCa development, although further experimental validation may be necessary.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 1","pages":"14-23"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12981719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of sperm cryopreservation and preparation before freezing-thawing process: A study of DNA fragmentation and ultrastructure.","authors":"Silvia Werdhy Lestari, Manggiasih Dwiayu Larasati, Zuraida Zuraida, Gita Pratama, Mulyoto Pangestu, Aufa Raihana, Arya Nandhana Syariendrar, Rafi Akbar Putranto","doi":"10.4103/JAPTR.JAPTR_221_25","DOIUrl":"10.4103/JAPTR.JAPTR_221_25","url":null,"abstract":"<p><p>Male fertility preservation can be achieved through assisted reproductive technology, in which sperm cryopreservation plays an essential role. However, the freezing-thawing process induces cellular stress, primarily through intracellular ice crystal formation, which damages membranes, organelles, and exacerbates osmotic and oxidative stress. This study aims to determine whether sperm cryopreservation and preparation affect sperm concentration and motility, cryosurvival rate (CSR), DNA fragmentation, and ultrastructure after thawing. A total of 10 human sperm samples were rapidly frozen in cryovials, with prefreeze and postthaw analyses compared against fresh controls, assessing sperm parameters, DNA fragmentation, and ultrastructure (scanning electron microscope). Statistical analysis included paired comparisons for two groups and multigroup tests with <i>post hoc</i> analysis. CSR was compared to 100% using a one-sample <i>t</i>-test. <i>P</i> < 0.05 was considered statistically significant. Sperm concentration decreased both after washing and after thawing compared to before freezing, while motility increased after washing, but it significantly decreased after thawing. The sperm motility result was confirmed by a significantly reduced CSR and DNA fragmentation index, which significantly increased at after thawing. Ultrastructure analysis also revealed more sperm damage after thawing. Cryopreservation impairs sperm motility, CSR, DNA fragmentation, and ultrastructure. Since <i>in vitro</i> fertilization requires sperm with good morphology and motility, new strategies are needed to minimize cryodamage.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 1","pages":"31-36"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12981717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the therapeutic effects of 10% bangun-bangun (<i>Coleus amboinicus</i>) leaf gel on second-degree burn healing in Wistar rats.","authors":"Yunita Sari Pane, Sufitni Sufitni, Sana Noreen","doi":"10.4103/JAPTR.JAPTR_163_25","DOIUrl":"10.4103/JAPTR.JAPTR_163_25","url":null,"abstract":"<p><p>Burns are traumatic injuries that compromise the skin's natural barrier, initiating a complex process of inflammation, proliferation, and tissue maturation. Second-degree burns, in particular, cause extensive tissue damage and require effective treatments that both accelerate healing and reduce pain. Traditionally, the Karo community has used <i>Bangun-Bangun</i> (<i>Coleus amboinicus</i>) leaves for wound care, owing to their analgesic and anti-inflammatory properties. This study aimed to investigate the therapeutic potential of a 10% Bangun-Bangun leaf extract (BBE) gel in managing pain and enhancing wound healing in Wistar rats with second-degree burns. It was hypothesized that BBE gel would provide better pain relief and faster recovery compared to standard therapies. A total of 54 Wistar rats were divided into three main groups: Base gel (negative control), Bioplacenton® (positive control), and 10% BBE gel (treatment). Each group was further separated into subgroups based on treatment duration (days 1-3, 1-14, and 1-21). Treatments were administered twice daily for 21 days. Pain levels were measured using a standard pain scale, whereas leukocyte migration was assessed through hematoxylin and eosin staining. Data were analyzed using SPSS with one-way analysis of variance and Bonferroni <i>post hoc</i> tests for normally distributed data, whereas nonparametric data were evaluated with the Kruskal-Wallis test. Pain reduction in the BBE gel group was not significantly different from Bioplacenton® (<i>P</i> = 0.139). However, rats treated with BBE gel (Group-III) demonstrated significantly lower leukocyte counts compared to controls (<i>P</i> = 0.010 for Group-I; <i>P</i> = 0.006 for Group-II), indicating improved inflammation control. Macroscopically, Group-III exhibited the fastest wound closure. A 10% BBE gel may serve as a promising, natural topical agent for second-degree burns by regulating inflammation and promoting healing. Further studies are required to explore its molecular mechanisms and safety profile.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 1","pages":"37-43"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12981708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of the ethanolic extract from the Benjakul remedy against oxidative stress and inflammation.","authors":"Worawat Surarit, Nitra Nuengchamnong, Srisopa Ruangnoo, Pakakrong Thongdeeying, Pintusorn Hansakul","doi":"10.4103/JAPTR.JAPTR_255_25","DOIUrl":"10.4103/JAPTR.JAPTR_255_25","url":null,"abstract":"<p><p>Oxidative stress and inflammation contribute to liver damage from xenobiotics, leading to chronic diseases. The ethanolic extract of the Benjakul remedy (BJE), a traditional Thai medicine, is recognized for its potential antioxidant and anti-inflammatory effects, though scientific validation is limited. This study assessed BJE's antioxidant effects against various radicals using chemical and cellular assays, evaluated its anti-inflammatory effects by measuring inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) levels, and determined protective effects by evaluating cell viability and oxidative damage. Total phenolic content was measured colorimetrically, and compounds were identified using liquid chromatography combined with electrospray ionization quadrupole time-of-flight mass spectrometry. BJE significantly scavenged synthetic radicals, nitric oxide radicals, and lipid radicals, with IC₅₀ values ranging from 28.76 to 113.46 μg/mL, and diminished ferrous ions with an IC₅₀ value of 1.48 ± 0.09 mg/mL. Furthermore, BJE potently neutralized superoxide anions and nitric oxide radicals within cells, yielding IC₅₀ values of 44.43 ± 6.79 and 15.89 ± 0.93 μg/mL, respectively. BJE reduced iNOS expression by 90% and COX-2 by 60% in a dose-dependent manner. BJE protected liver BNL CL.2 cells from oxidative stress and inflammation induced by pyrogallol, improving cell viability, decreasing oxidative damage, and restoring critical antioxidant enzyme activities. BJE contained 6-gingerol, alkaloid amides, and nonalkaloid amides. This study marks the antioxidant and anti-inflammatory effects of BJE at the molecular levels.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 1","pages":"1-7"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12981716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147468031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nephroprotective effect of lercanidipine against gentamicin-induced kidney damage.","authors":"Maryam H Shubbar, Hussam H Kadhum","doi":"10.4103/JAPTR.JAPTR_243_25","DOIUrl":"10.4103/JAPTR.JAPTR_243_25","url":null,"abstract":"<p><p>Gentamicin, an aminoglycoside antibiotic, induces nephrotoxicity mainly through oxidative stress. This study evaluated the nephroprotective potential of lercanidipine, a calcium-channel blocker, against gentamicin-induced renal injury in rats. Adult Sprague-Dawley rats were randomly divided into four groups: control, lercanidipine (3 mg/kg/day, p.o.), gentamicin (50 mg/kg/day, i.m.), and a combination group pretreated with lercanidipine for 5 days followed by concurrent gentamicin for 5 days. Serum urea, creatinine, sodium (Na⁺), and potassium (K⁺) were determined, and kidney homogenates were analyzed for malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD). Gentamicin treatment produced marked renal dysfunction, with elevated serum urea and creatinine, increased Na ⁺ by 29%, and decreased K ⁺ by 27.5% relative to control, accompanied by a 37% reduction in urine volume. Co-treatment with lercanidipine restored electrolyte balance, lowering Na ⁺ by 20% and raising K ⁺ by 27% compared with gentamicin alone, while increasing urine volume by 42%. Lercanidipine also markedly attenuated oxidative stress, reducing MDA by about 65% and increasing SOD and GSH activities by approximately 183% and 140%, respectively, relative to gentamicin alone. These findings demonstrate that lercanidipine significantly protects against gentamicin-induced nephrotoxicity by improving renal function, restoring electrolyte homeostasis, and enhancing the antioxidant defence system.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 1","pages":"8-13"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12981715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147468034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic profiling and regenerative potential of bovine placental extract in wound healing models.","authors":"Julian Arbey Gonzalez Ospina, Erika M Muñoz Martinez, Sebastian Hernandez Sampa, Juan S Gomez, Mauricio Palacios, Alvaro A Barrera-Ocampo","doi":"10.4103/JAPTR.JAPTR_157_25","DOIUrl":"10.4103/JAPTR.JAPTR_157_25","url":null,"abstract":"<p><p>The placenta is rich in proteins and metabolites that modulate repair processes. Bovine placental extract (BPE) offers a practical alternative to human-derived materials for regenerative applications. The study aimed to characterize the proteomic composition of BPE and evaluate its regenerative potential <i>in vitro</i> and <i>in vivo</i>. Proteins were profiled by liquid chromatography-tandem mass spectrometry and analyzed through bioinformatic enrichment of signaling pathways. Regenerative activity was assessed in New Zealand rabbit excisional wounds and human fibroblast cultures using viability and scratch assays. A total of 204 proteins were identified, including 89 differentially expressed proteins enriched in transforming growth factor-β, WNT, and extracellular matrix pathways. Topical BPE accelerated wound closure in rabbits compared with saline controls (<i>P</i> < 0.05). In fibroblasts, viability remained above 80% across all concentrations, and migration showed a nonsignificant dose-dependent trend. BPE contains bioactive proteins linked to key repair pathways and enhances wound healing without cytotoxic effects. These results support its potential as a safe, accessible biomaterial for regenerative medicine.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 1","pages":"71-77"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12981714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and nitric oxide production inhibitory activities of <i>Liriope platyphylla</i> and <i>Asparagus cochinchinensis</i> (<i>Liliaceae</i>) extracts.","authors":"Kyoung-Sun Seo, Kyeong Won Yun","doi":"10.4103/JAPTR.JAPTR_16_25","DOIUrl":"10.4103/JAPTR.JAPTR_16_25","url":null,"abstract":"<p><p><i>Liriope platyphylla</i> and <i>Asparagus cochinchinensis</i> have been utilized as adjunctive therapies for disorders involving immune dysfunction in Korea. This study investigated the antioxidant and nitric oxide (NO) production inhibitory activities in different extracts of the two plants. The antioxidant activity of the tuber-derived extracts was assessed <i>in vitro</i> 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The cell of RAW 264.7 macrophages were treated with extracts from two plants, and cell viability and nitric oxide (NO) production was determined using the MTT and Griess assay, respectively. The ethanol extract of <i>L. platyphylla</i> showed the highest antioxidant activity, showing the lowest IC₅₀ values of 132.14 ± 2.37 μg/mL in the DPPH assay and 183.82 ± 3.24 μg/mL in the ABTS assay. All tested concentrations of the ethanol extract from <i>L. platyphylla</i> maintained cell viability above 100%. Neither ethanol nor hot water extracts of the two plants induced cytotoxicity. Moreover, NO production in RAW 264.7 macrophages was inhibited in a concentration-dependent manner by the tested extracts. These results provide scientific backing for the traditional use of the two plants and suggest that the <i>in vitro</i> antioxidant and NO inhibitory properties may have potential applications in functional foods, pharmaceuticals, and cosmeceuticals.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 1","pages":"66-70"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12981711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemometric classification of red ginger (<i>Zingiber officinale</i> var. Rubrum) oleoresin from different regions using Fourier-transform infrared-ATR spectroscopy.","authors":"Arif Nurmansyah, Shaum Shiyan, Yudi Arina, Iyan Sopyan, Muchtaridi Muchtaridi","doi":"10.4103/JAPTR.JAPTR_175_25","DOIUrl":"10.4103/JAPTR.JAPTR_175_25","url":null,"abstract":"<p><p>Red ginger contains valuable oleoresins, primarily <i>gengerols, shogaol</i>, and <i>zingerone</i>. However, its chemical profile can vary by geographic origin. This study aimed to classify the red ginger samples from different regions based on their chemical characteristics. Ethanol extracts of red ginger were prepared via maceration. Chemical profiles were obtained using Fourier-transform infrared (FTIR) spectroscopy, and the data were analyzed using principal component analysis-correspondence analysis (PCA-CA) chemometrics for classification. PCA-CA analysis revealed the highest similarity between samples from Pematang Simalungun and Sidomulyo Jalur 18 villages. When compared against a <i>zingerone</i> standard, only the sample from pagerharjo village showed a close chemical relationship. FTIR combined with PCA-CA chemometrics effectively distinguishes red ginger based on its chemical composition and geographic origin.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 1","pages":"51-58"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12981718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Investigation of antioxidant, anti collagenase, anti-elastase, and anti-hyaluronidase activities of <i>Solanum trilobatum</i> L. for pharmaceutical development.","authors":"","doi":"10.4103/JAPTR.JAPTR_32_26","DOIUrl":"10.4103/JAPTR.JAPTR_32_26","url":null,"abstract":"<p><p>[This corrects the article on p. 201 in vol. 16, PMID: 41234353.].</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"17 1","pages":"85"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12981713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of variables on buccal mucoadhesive film-forming spray for clotrimazole.","authors":"Nidhal Khazaal Maraie, Reem Wael Shahadha","doi":"10.4103/JAPTR.JAPTR_83_25","DOIUrl":"10.4103/JAPTR.JAPTR_83_25","url":null,"abstract":"<p><p>Buccal spray is an attractive dosage form for its ease of application, especially for infant and geriatric patients. The aim in this work was to optimize and prepare a clotrimazole buccal spray through exploring the role of main variables producing a mucoadhesive film, to evaluate its effectiveness against <i>Candida albicans</i>. The method of preparation involved solubilizing the drug using a suitable amount of PEG as well as PG, utilizing different most commonly applied bioadhesive polymers to achieve buccal mucoadhesion and compromising the viscosity. The solution was conserved in a plastic container with a metered dose designed to give 0.2 ml per puff. The optimized formula containing 0.5% polyvinyl alcohol, 60% PEG, and 15% PG exhibited suitable pH (6.16 ± 0.002), high drug release (100% ±1.098% within 90 min), drug content (104.1% ±2.001%), volume per actuation (0.19 ± 0.010 ml), spray of angle (47.2° ±0.896°) and acceptable viscosity (74 ± 1.3 cp), superior anti-<i>Candida</i> activity than commercial product with no irritation. Overall, choosing suitable additives can compromise the viscosity and have a great effect in obtaining the required dose upon a certain accurate value of actuations utilizing metered dose spray, enabling the product to flow out as ultrafine droplets instantly spread and adhere to buccal mucosa, improving residence time and diffusion, hence enhancing the efficacy of clotrimazole against candidiasis.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":"16 4","pages":"183-189"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12610290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145512763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}