ISRN Microbiology最新文献_第3页

Growth Limiting pH, Water Activity, and Temperature for Neurotoxigenic Strains of Clostridium butyricum. 丁酸梭菌神经毒素菌株的生长限制pH、水活度和温度。

ISRN Microbiology Pub Date : 2013-09-30 eCollection Date: 2013-01-01 DOI: 10.1155/2013/731430

Hamid B Ghoddusi, Richard E Sherburn, Olusimbo O Aboaba

{"title":"Growth Limiting pH, Water Activity, and Temperature for Neurotoxigenic Strains of Clostridium butyricum.","authors":"Hamid B Ghoddusi, Richard E Sherburn, Olusimbo O Aboaba","doi":"10.1155/2013/731430","DOIUrl":"https://doi.org/10.1155/2013/731430","url":null,"abstract":"Some rare strains of Clostridium butyricum carry the gene encoding the botulinal type E neurotoxin and must be considered as possible hazards in certain types of food. The limiting growth conditions for C. butyricum were determined in peptone yeast glucose starch (PYGS) broth incubated anaerobically at 30°C for up to 42 days. The minimum pH values permitting growth depended on the acidulant and strain. Organic acids were more effective at inhibiting growth than HCl as expected. The lowest pH values at which growth of toxigenic and nontoxigenic strains of C. butyricum was observed in broth acidified with HCl were 4.1 and 4.2, respectively. In organic acids, however, the minimum pH varied between 4.4 and 5.1 depending on acid type and concentration. The minimum water activity for growth of toxigenic strains of C. butyricum was 0.96. The minimum growth temperatures of the toxigenic strains of C. butyricum (ca 10-11°C) were somewhat higher than for non-toxigenic ones (8°C). It was concluded that control of toxigenic C. butyricum in the food industry needs to allow for the greater pH tolerance of this species compared with proteolytic C. botulinum. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2013 ","pages":"731430"},"PeriodicalIF":0.0,"publicationDate":"2013-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/731430","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31835867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Viable but nonculturable bacteria: food safety and public health perspective. 可存活但不可培养的细菌:食品安全和公共卫生观点。

ISRN Microbiology Pub Date : 2013-09-26 DOI: 10.1155/2013/703813

Md Fakruddin, Khanjada Shahnewaj Bin Mannan, Stewart Andrews

引用次数: 143

The effect of gold and iron-oxide nanoparticles on biofilm-forming pathogens. 金和氧化铁纳米粒子对形成生物膜的病原体的影响。

ISRN Microbiology Pub Date : 2013-09-25 eCollection Date: 2013-01-01 DOI: 10.1155/2013/272086

Madhu Bala Sathyanarayanan, Reneta Balachandranath, Yuvasri Genji Srinivasulu, Sathish Kumar Kannaiyan, Guruprakash Subbiahdoss

引用次数: 0

Production of a Lipopeptide Biosurfactant by a Novel Bacillus sp. and Its Applicability to Enhanced Oil Recovery. 一种新型芽孢杆菌生产脂肽类生物表面活性剂及其对提高采收率的适用性。

ISRN Microbiology Pub Date : 2013-09-24 eCollection Date: 2013-01-01 DOI: 10.1155/2013/621519

Thivaharan Varadavenkatesan, Vytla Ramachandra Murty

{"title":"Production of a Lipopeptide Biosurfactant by a Novel Bacillus sp. and Its Applicability to Enhanced Oil Recovery.","authors":"Thivaharan Varadavenkatesan, Vytla Ramachandra Murty","doi":"10.1155/2013/621519","DOIUrl":"https://doi.org/10.1155/2013/621519","url":null,"abstract":"Biosurfactants are surface-active compounds derived from varied microbial sources including bacteria and fungi. They are secreted extracellularly and have a wide range of exciting properties for bioremediation purposes. They also have vast applications in the food and medicine industry. With an objective of isolating microorganisms for enhanced oil recovery (EOR) operations, the study involved screening of organisms from an oil-contaminated site. Morphological, biochemical, and 16S rRNA analysis of the most promising candidate revealed it to be Bacillus siamensis, which has been associated with biosurfactant production, for the first time. Initial fermentation studies using mineral salt medium supplemented with crude oil resulted in a maximum biosurfactant yield of 0.64 g/L and reduction of surface tension to 36.1 mN/m at 96 h. Characterization studies were done using thin layer chromatography and Fourier transform infrared spectroscopy. FTIR spectra indicated the presence of carbonyl groups, alkyl bonds, and C-H and N-H stretching vibrations, typical of peptides. The extracted biosurfactant was stable at extreme temperatures, pH, and salinity. Its applicability to EOR was further verified by conducting sand pack column studies that yielded up to 60% oil recovery. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2013 ","pages":"621519"},"PeriodicalIF":0.0,"publicationDate":"2013-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/621519","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31843987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 85

16S rRNA PCR-Denaturing Gradient Gel Electrophoresis of Oral Lactobacillus casei Group and Their Phenotypic Appearances. 口腔干酪乳杆菌群的16S rRNA pcr -变性梯度凝胶电泳及其表型表现。

ISRN Microbiology Pub Date : 2013-09-23 eCollection Date: 2013-01-01 DOI: 10.1155/2013/342082

S Piwat, R Teanpaisan

{"title":"16S rRNA PCR-Denaturing Gradient Gel Electrophoresis of Oral Lactobacillus casei Group and Their Phenotypic Appearances.","authors":"S Piwat, R Teanpaisan","doi":"10.1155/2013/342082","DOIUrl":"https://doi.org/10.1155/2013/342082","url":null,"abstract":"This study aimed to develop a 16S rRNA PCR-denaturing gradient gel electrophoresis (DGGE) to identify the species level of Lactobacillus casei group and to investigate their characteristics of acid production and inhibitory effect. PCR-DGGE has been developed based on the 16S rRNA gene, and a set of HDA-1-GC and HDA-2, designed at V2-V3 region, and another set of CARP-1-GC and CARP-2, designed at V1 region, have been used. The bacterial strains included L. casei ATCC 393, L. paracasei CCUG 32212, L. rhamnosus ATCC 7469, L. zeae CCUG 35515, and 46 clinical strains of L. casei/paracasei/rhamnosus. Inhibitory effect against Streptococcus mutans and acid production were examined. Results revealed that each type species strain and identified clinical isolate showed its own unique DGGE pattern using CARP1-GC and CARP2 primers. HDA1-GC and HDA2 primers could distinguish the strains of L. paracasei from L. casei. It was found that inhibitory effect of L. paracasei was stronger than L. casei and L. rhamnosus. The acid production of L. paracasei was lower than L. casei and L. rhamnosus. In conclusion, the technique has been proven to be able to differentiate between closely related species in L. casei group and thus provide reliable information of their phenotypic appearances. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2013 ","pages":"342082"},"PeriodicalIF":0.0,"publicationDate":"2013-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/342082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31834132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Admission Screening of Methicillin-Resistant Staphylococcus aureus with Rapid Molecular Detection in Intensive Care Unit: A Three-Year Single-Centre Experience in Hong Kong. 用快速分子检测法筛查重症监护室耐甲氧西林金黄色葡萄球菌的入院情况:香港单中心三年经验

ISRN Microbiology Pub Date : 2013-09-19 eCollection Date: 2013-01-01 DOI: 10.1155/2013/140294

Eddie Chi Man Leung, May Kin Ping Lee, Raymond Wai Man Lai

{"title":"Admission Screening of Methicillin-Resistant Staphylococcus aureus with Rapid Molecular Detection in Intensive Care Unit: A Three-Year Single-Centre Experience in Hong Kong.","authors":"Eddie Chi Man Leung, May Kin Ping Lee, Raymond Wai Man Lai","doi":"10.1155/2013/140294","DOIUrl":"https://doi.org/10.1155/2013/140294","url":null,"abstract":"Background. The admission screening of methicillin-resistant Staphylococcus aureus (MRSA) by rapid molecular assay is considered to be an effective method in reducing the transmission of MRSA in intensive care unit (ICU). Method. The admission screening on patients from ICU once on their admissions by BD GeneOhm MRSA assay has been introduced to Prince of Wales Hospital, Hong Kong, since 2008. The assay was performed on weekdays and reported on the day of testing. Patients pending for results were under standard precautions until the negative screening results were notified, while contact precautions were implemented for MRSA-positive patients. In this study, we compared the MRSA transmission rate in molecular screening periods (2008 to 2010) with the historical culture periods (2006 to 2007) as control. Results. A total of 4679 samples were tested; the average carriage rate of MRSA on admission was 4.45%. By comparing with the historical culture periods, the mean incidence ICU-acquired MRSA infection was reduced from 3.67 to 1.73 per 1000 patient bed days. Conclusion. The implementation of admission screening of MRSA with molecular method in intensive care unit could reduce the MRSA transmission, especially in the area with high MRSA prevalence situation in Hong Kong. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":" ","pages":"140294"},"PeriodicalIF":0.0,"publicationDate":"2013-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/140294","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40276855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

β -Lactamase-Producing Multidrug-Resistant Bacterial Pathogens from Tracheal Aspirates of Intensive Care Unit Patients at National Institute of Neurological and Allied Sciences, Nepal. 尼泊尔国家神经和相关科学研究所重症监护病房患者气管吸入物中产生β -内酰胺酶的多重耐药细菌病原体。

ISRN Microbiology Pub Date : 2013-09-02 eCollection Date: 2013-01-01 DOI: 10.1155/2013/847569

Santosh Khanal, Dev Raj Joshi, Dwij Raj Bhatta, Upendra Devkota, Bharat Mani Pokhrel

{"title":"β -Lactamase-Producing Multidrug-Resistant Bacterial Pathogens from Tracheal Aspirates of Intensive Care Unit Patients at National Institute of Neurological and Allied Sciences, Nepal.","authors":"Santosh Khanal, Dev Raj Joshi, Dwij Raj Bhatta, Upendra Devkota, Bharat Mani Pokhrel","doi":"10.1155/2013/847569","DOIUrl":"https://doi.org/10.1155/2013/847569","url":null,"abstract":"The widespread use of tracheal intubation and mechanical ventilation to support the critically ill patients increases the risk of development of tracheobronchitis and bronchopneumonia. This cross-sectional study was conducted with an aim to isolate and identify bacterial pathogens from tracheal aspirates producing extended-spectrum β -lactamase (ESBL), AmpC β -lactamase, and metallo- β -lactamase (MBL) from August 2011 to April 2012 at National Institute of Neurological and Allied Sciences (NINAS), Kathmandu, Nepal. ESBL was detected by combined disk assay using cefotaxime and cefotaxime with clavulanate, AmpC β -lactamase by inhibitor-based method using cefoxitin and phenylboronic acid, and MBL by Imipenem-EDTA combined disk method. 167 bacterial strains were isolated from 187 samples and majority of them were Acinetobacter spp. followed by Klebsiella pneumoniae with 32.9% and 25.1%, respectively. 68.8% of isolates were multidrug resistant (MDR) and Acinetobacter spp. constituted 85.4%. ESBL, AmpC β -lactamase, and MBL were detected in 35 (25%), 51 (37.2%), and 11 (36.7%) isolates, respectively. Pseudomonas spp. (42.8%) were the predominant ESBL producer while Acinetobacter spp. were the major AmpC β -lactamase producer (43.1%) and MBL producer (54.5%). ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2013 ","pages":"847569"},"PeriodicalIF":0.0,"publicationDate":"2013-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/847569","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31768882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 56

The effect of curcumin and cotrimoxazole in salmonella typhimurium infection in vivo. 姜黄素和复方新诺明对鼠伤寒沙门菌体内感染的影响。

ISRN Microbiology Pub Date : 2013-08-29 eCollection Date: 2013-01-01 DOI: 10.1155/2013/601076

Siwipeni Irmawanti Rahayu, Nurdiana Nurdiana, Sanarto Santoso

引用次数: 10

Role of flies as vectors of foodborne pathogens in rural areas. 农村地区苍蝇作为食源性病原体载体的作用。

ISRN Microbiology Pub Date : 2013-08-04 eCollection Date: 2013-01-01 DOI: 10.1155/2013/718780

Cláudia Barreiro, Helena Albano, Joana Silva, Paula Teixeira

{"title":"Role of flies as vectors of foodborne pathogens in rural areas.","authors":"Cláudia Barreiro, Helena Albano, Joana Silva, Paula Teixeira","doi":"10.1155/2013/718780","DOIUrl":"10.1155/2013/718780","url":null,"abstract":"This study aims to evaluate flies as a vector for foodborne pathogens. For this purpose, several flies were collected from different sites from rural areas. These flies were then analyzed for the presence of Enterobacteriaceae, Escherichia coli, Staphylococcus coagulase positive, and Listeria monocytogenes. Another aim of this study was to evaluate some virulence factors of the collected pathogens: susceptibility to some antibiotics and the presence of enterotoxigenic S. aureus. The results showed that flies in the presence of animals demonstrated a significantly higher prevalence of the studied pathogens than those collected in the kitchens, and kitchens situated in the closest proximity to the animal husbandry had a higher count than the kitchens in private houses. Enterobacteriaceae was the indicator organism with the highest microbial counts followed by E. coli and S. aureus. Listeria monocytogenes was not detected from any of the collected flies. The antimicrobial susceptibility test showed that the bacteria carried by the flies possessed multiantibiotic resistance profiles, and enterotoxin A was produced by 17.9% of the confirmed S. aureus isolates. These results demonstrate that flies can transmit foodborne pathogens and their associated toxin and resistance and the areas of higher risk are those in closer proximity to animal production sites. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2013 ","pages":"718780"},"PeriodicalIF":0.0,"publicationDate":"2013-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31689615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host-Microbe Interactions in Caenorhabditis elegans. 秀丽隐杆线虫中宿主-微生物的相互作用。

ISRN Microbiology Pub Date : 2013-08-01 eCollection Date: 2013-01-01 DOI: 10.1155/2013/356451

Rui Zhang, Aixin Hou

引用次数: 51