ISRN Microbiology最新文献_第2页

Prevalence of haemoplasma infections in stray cats in northern Italy. 意大利北部流浪猫血原体感染的流行情况。

ISRN Microbiology Pub Date : 2014-02-23 eCollection Date: 2014-01-01 DOI: 10.1155/2014/298352

Eva Spada, Daniela Proverbio, Paola Galluzzo, Alessandra Della Pepa, Giada Bagnagatti De Giorgi, Roberta Perego, Elisabetta Ferro

{"title":"Prevalence of haemoplasma infections in stray cats in northern Italy.","authors":"Eva Spada, Daniela Proverbio, Paola Galluzzo, Alessandra Della Pepa, Giada Bagnagatti De Giorgi, Roberta Perego, Elisabetta Ferro","doi":"10.1155/2014/298352","DOIUrl":"https://doi.org/10.1155/2014/298352","url":null,"abstract":"This study investigated the prevalence of feline haemoplasma infections in a number of stray cat colonies in Milan, Northern Italy. Blood samples from 260 stray cats were evaluated, with conventional PCR, for the presence of DNA associated with Mycoplasma haemofelis (Mhf) and \"Candidatus Mycoplasma haemominutum\" (CMhm). Odd ratios (OR) were calculated to identify risk factors for haemoplasma infections. PCR was positive in 86 out of 260 subjects (33.1%), with a prevalence of 10.8% (28/260 cats) for Mhf and 22.3% (58/260 cats) for CMhm. No coinfections were registered. There were significant associations between infections and season of sampling, that is, a negative association between winter sampling and a haemoplasma positive status (OR = 0.29, P = 0.001), or CMhm positive status (OR = 0.29, P = 0.01). Haemoplasma infections are common in stray cats in Milan. Thus, domestic cats with outdoor access should be routinely monitored and treated for ectoparasites to minimize risks of disease acquisition. Moreover, as these infections are transmitted via blood, feline blood donors from this area should be screened by PCR and preferably be drawn from a population of indoor cats regularly treated for fleas. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2014 ","pages":"298352"},"PeriodicalIF":0.0,"publicationDate":"2014-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/298352","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32240732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Occurrence of Methicillin Resistant and Enterotoxigenic Staphylococcus aureus in Traditional Cheeses in the North West of Iran. 伊朗西北部传统奶酪中耐药甲氧西林金黄色葡萄球菌和肠毒性金黄色葡萄球菌的发生率

ISRN Microbiology Pub Date : 2014-02-13 eCollection Date: 2014-01-01 DOI: 10.1155/2014/129580

Dariush Shanehbandi, Behzad Baradaran, Saeed Sadigh-Eteghad, Habib Zarredar

{"title":"Occurrence of Methicillin Resistant and Enterotoxigenic Staphylococcus aureus in Traditional Cheeses in the North West of Iran.","authors":"Dariush Shanehbandi, Behzad Baradaran, Saeed Sadigh-Eteghad, Habib Zarredar","doi":"10.1155/2014/129580","DOIUrl":"10.1155/2014/129580","url":null,"abstract":"Traditional dairy products are potential sources of a variety of microorganisms which participate in food poisoning. Staphylococcus aureus is a conspicuous example of toxigenic bacteria causative for food-borne diseases. Moreover, resistance to methicillin is a prominent index in food hygiene studies. In the present study, we have aimed at characterization and identification of enterotoxigenic methicillin resistant S. aureus (MRSA) isolated from traditional cheeses in Azerbaijan region in the northwest of Iran during 2012. A number of phenotypical and molecular assays were utilized for screening of S. aureus. Subsequently, the prevalence of the genes responsible for the five staphylococcal enterotoxins (SEA-SEE) and also methicillin resistance gene was assessed. The outcomes of phenotypical methods were in conformity with those of the molecular procedures. The results indicated that 16% of cheese samples were contaminated by S. aureus. 110 isolates were authenticated by both phenotypical and molecular methods. All of the mentioned isolates were positive for coa, nuc, and 16S rDNA primers. 21% of these isolates were mecA positive and 60.8% of these MRSA were positive for SEs. Regarding the frequent outbreaks of enterotoxigenic MRSA, new hygiene policies and management practices should be considered to increase food safety and avoid extra treatment costs. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2014 ","pages":"129580"},"PeriodicalIF":0.0,"publicationDate":"2014-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32227548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mode of Action and Specificity of Bacillus thuringiensis Toxins in the Control of Caterpillars and Stink Bugs in Soybean Culture. 苏云金芽孢杆菌毒素在大豆栽培中防治毛虫和臭虫的作用方式和特异性。

ISRN Microbiology Pub Date : 2014-01-20 eCollection Date: 2014-01-01 DOI: 10.1155/2014/135675

Rogério Schünemann, Neiva Knaak, Lidia Mariana Fiuza

{"title":"Mode of Action and Specificity of Bacillus thuringiensis Toxins in the Control of Caterpillars and Stink Bugs in Soybean Culture.","authors":"Rogério Schünemann, Neiva Knaak, Lidia Mariana Fiuza","doi":"10.1155/2014/135675","DOIUrl":"https://doi.org/10.1155/2014/135675","url":null,"abstract":"The bacterium Bacillus thuringiensis (Bt) produces delta-endotoxins that possess toxic properties and can be used as biopesticides, as well as a source of genes for the construction of transgenic plants resistant to insects. In Brazil, the introduction of Bt soybean with insecticidal properties to the velvetbean caterpillar, the main insect pest of soybean, has been seen a promising tool in the management of these agroecosystems. However, the increase in stink bug populations in this culture, in various regions of the country, which are not susceptible to the existing genetically modified plants, requires application of chemicals that damage the environment. Little is known about the actual toxicity of Bt to Hemiptera, since these insects present sucking mouthparts, which hamper toxicity assays with artificial diets containing toxins of this bacterium. In recent studies of cytotoxicity with the gut of different hemipterans, susceptibility in the mechanism of action of delta-endotoxins has been demonstrated, which can generate promising subsidies for the control of these insect pests in soybean. This paper aims to review the studies related to the selection, application and mode of action of Bt in the biological control of the major pest of soybean, Anticarsia gemmatalis, and an analysis of advances in research on the use of Bt for control hemipterans. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2014 ","pages":"135675"},"PeriodicalIF":0.0,"publicationDate":"2014-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/135675","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32157876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 110

Canola Cake as a Potential Substrate for Proteolytic Enzymes Production by a Selected Strain of Aspergillus oryzae: Selection of Process Conditions and Product Characterization. 将油菜籽饼作为一种潜在的底物，供一种精选的黑曲霉菌株生产蛋白水解酶：工艺条件的选择和产品特性。

ISRN Microbiology Pub Date : 2013-12-25 DOI: 10.1155/2013/369082

Adriana C Freitas, Ruann J S Castro, Maria A Fontenele, Antonio S Egito, Cristiane S Farinas, Gustavo A S Pinto

{"title":"Canola Cake as a Potential Substrate for Proteolytic Enzymes Production by a Selected Strain of Aspergillus oryzae: Selection of Process Conditions and Product Characterization.","authors":"Adriana C Freitas, Ruann J S Castro, Maria A Fontenele, Antonio S Egito, Cristiane S Farinas, Gustavo A S Pinto","doi":"10.1155/2013/369082","DOIUrl":"10.1155/2013/369082","url":null,"abstract":"Oil cakes have excellent nutritional value and offer considerable potential for use in biotechnological processes that employ solid-state fermentation (SSF) for the production of high value products. This work evaluates the feasibility of using canola cake as a substrate for protease production by a selected strain of Aspergillus oryzae cultivated under SSF. The influences of the following process parameters were considered: initial substrate moisture content, incubation temperature, inoculum size, and pH of the buffer used for protease extraction and activity analysis. Maximum protease activity was obtained after cultivating Aspergillus oryzae CCBP 001 at 20°C, using an inoculum size of 10(7) spores/g in canola cake medium moistened with 40 mL of water to 100 g of cake. Cultivation and extraction under selected conditions increased protease activity 5.8-fold, compared to the initial conditions. Zymogram analysis of the enzymatic extract showed that the protease molecular weights varied between 31 and 200 kDa. The concentrated protease extract induced clotting of casein in 5 min. The results demonstrate the potential application of canola cake for protease production under SSF and contribute to the technological advances needed to increase the efficiency of processes designed to add value to agroindustrial wastes. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2013 ","pages":"369082"},"PeriodicalIF":0.0,"publicationDate":"2013-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32055761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Multidrug Resistant Shigella and Vibrio cholerae O1 Infections in Patients Treated at an Urban and a Rural Hospital in Bangladesh. 孟加拉国城市和农村医院患者多重耐药志贺氏菌和霍乱弧菌感染的特点

ISRN Microbiology Pub Date : 2013-12-22 DOI: 10.1155/2013/213915

Sumon Kumar Das, Erik H Klontz, Ishrat J Azmi, Abu I M S Ud-Din, Mohammod Jobayer Chisti, Mokibul Hassan Afrad, Mohammad Abdul Malek, Shahnawaz Ahmed, Jui Das, Kaisar Ali Talukder, Mohammed Abdus Salam, Pradip Kumar Bardhan, Abu Syed Golam Faruque, Karl C Klontz

{"title":"Characteristics of Multidrug Resistant Shigella and Vibrio cholerae O1 Infections in Patients Treated at an Urban and a Rural Hospital in Bangladesh.","authors":"Sumon Kumar Das, Erik H Klontz, Ishrat J Azmi, Abu I M S Ud-Din, Mohammod Jobayer Chisti, Mokibul Hassan Afrad, Mohammad Abdul Malek, Shahnawaz Ahmed, Jui Das, Kaisar Ali Talukder, Mohammed Abdus Salam, Pradip Kumar Bardhan, Abu Syed Golam Faruque, Karl C Klontz","doi":"10.1155/2013/213915","DOIUrl":"https://doi.org/10.1155/2013/213915","url":null,"abstract":"We determined the frequency of multidrug resistant (MDR) infections with Shigella spp. and Vibrio cholerae O1 at an urban (Dhaka) and rural (Matlab) hospital in Bangladesh. We also compared sociodemographic and clinical features of patients with MDR infections to those with antibiotic-susceptible infections at both sites. Analyses were conducted using surveillance data from the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), for the years 2000-2012. Compared to patients with antibiotic-susceptible for Shigella infections, those in Dhaka with MDR shigellosis were more likely to experience diarrhea for >24 hours, while, in Matlab, they were more likely to stay inhospital >24 hours. For MDR shigellosis, Dhaka patients were more likely than those in Matlab to have dehydration, stool frequency >10/day, and diarrheal duration >24 hours. Patients with MDR Vibrio cholerae O1 infections in Dhaka were more likely than those in Matlab to experience dehydration and stool frequency >10/day. Thus, patients with MDR shigellosis and Vibrio cholerae O1 infection exhibited features suggesting more severe illness than those with antibiotic-susceptible infections. Moreover, Dhaka patients with MDR shigellosis and Vibrio cholerae O1 infections exhibited features indicating more severe illness than patients in Matlab. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2013 ","pages":"213915"},"PeriodicalIF":0.0,"publicationDate":"2013-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/213915","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32056365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Growth of Mycobacterium avium subsp. paratuberculosis, Escherichia coli, and Salmonella Enteritidis during Preparation and Storage of Yogurt. 鸟分枝杆菌亚种的生长。酸奶制备和储存过程中的副结核杆菌、大肠杆菌和肠炎沙门氏菌。

ISRN Microbiology Pub Date : 2013-12-16 DOI: 10.1155/2013/247018

K Cirone, Y Huberman, C Morsella, L Méndez, M Jorge, F Paolicchi

{"title":"Growth of Mycobacterium avium subsp. paratuberculosis, Escherichia coli, and Salmonella Enteritidis during Preparation and Storage of Yogurt.","authors":"K Cirone, Y Huberman, C Morsella, L Méndez, M Jorge, F Paolicchi","doi":"10.1155/2013/247018","DOIUrl":"10.1155/2013/247018","url":null,"abstract":"The purpose of this study was to determine the viability of Mycobacterium avium subsp. paratuberculosis (MAP), Escherichia coli (E. coli), and Salmonella Enteritidis (S. Enteritidis) during preparation and refrigerated storage of yogurt. Three yogurts were prepared using pasteurized commercial milk. Each yogurt was artificially contaminated with (1) MAP, (2) E. coli + S. Enteritidis, and (3) MAP + E. coli + S. Enteritidis. Samples were taken during and after the fermentation process until day 20 after inoculation. MAP was not detected during their preparation and short-term storage but was recuperated after starting at 180 min after inoculation storage. Live bacterial counts of E. coli, and S. Enteritidis increased during the first 24 hours, followed by a slight decrease towards the end of the study. In this study it was shown how MAP, E. coli, and S. Enteritidis resisted the acidic conditions generated during the preparation of yogurt and low storage temperatures. This work contributes to current knowledge regarding survival of MAP, E. coli, and S. Enteritidis during preparation and refrigerated storage of yogurt and emphasizes the need to improve hygiene measures to ensure the absence of these pathogenic microorganisms in dairy products. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2013 ","pages":"247018"},"PeriodicalIF":0.0,"publicationDate":"2013-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32055760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of fungi isolated from environmental samples for xylanase and cellulase production. 产木聚糖酶和纤维素酶真菌的环境分离筛选。

ISRN Microbiology Pub Date : 2013-12-12 eCollection Date: 2013-01-01 DOI: 10.1155/2013/283423

Mohammed Inuwa Ja'afaru

引用次数: 54

Acoustic Emission Signal of Lactococcus lactis before and after Inhibition with NaN 3 and Infection with Bacteriophage c2. 乳球菌NaN 3抑制及c2噬菌体感染前后的声发射信号

ISRN Microbiology Pub Date : 2013-11-17 eCollection Date: 2013-01-01 DOI: 10.1155/2013/257313

Debasish Ghosh, John M Stencel, Clair D Hicks, Fred Payne, Didem Ozevin

{"title":"Acoustic Emission Signal of Lactococcus lactis before and after Inhibition with NaN 3 and Infection with Bacteriophage c2.","authors":"Debasish Ghosh, John M Stencel, Clair D Hicks, Fred Payne, Didem Ozevin","doi":"10.1155/2013/257313","DOIUrl":"https://doi.org/10.1155/2013/257313","url":null,"abstract":"The detection of acoustic emission (AE) from Lactococcus lactis, ssp lactis is reported in which emission intensities are used to follow and define metabolic activity during growth in nutrient broths. Optical density (OD) data were also acquired during L. lactis growth at 32°C and provided insight into the timing of the AE signals relative to the lag, logarithmic, and stationary growth phases of the bacteria. The inclusion of a metabolic inhibitor, NaN3, into the nutrient broth eliminated bacteria metabolic activity according to the OD data, the absence of which was confirmed using AE data acquisition. The OD and AE data were also acquired before and after the addition of Bacteriophage c2 in L. lactis containing nutrient broths during the early or middle logarithmic phase; c2 phage m.o.i. (Multiplicity of infection) was varied to help differentiate whether the detected AE was from bacteria cells during lysis or from the c2 phage during genome injection into the cells. It is proposed that AE measurements using piezoelectric sensors are sensitive enough to detect bacteria at the amount near 10(4) cfu/mL, to provide real time data on bacteria metabolic activity and to dynamically monitor phage infection of cells. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2013 ","pages":"257313"},"PeriodicalIF":0.0,"publicationDate":"2013-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/257313","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31965369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Distribution and antimicrobial susceptibility pattern of bacterial pathogens causing urinary tract infection in urban community of meerut city, India. 印度密鲁特市城市社区尿路感染病原菌分布及药敏模式

ISRN Microbiology Pub Date : 2013-10-29 eCollection Date: 2013-01-01 DOI: 10.1155/2013/749629

Devanand Prakash, Ramchandra Sahai Saxena

{"title":"Distribution and antimicrobial susceptibility pattern of bacterial pathogens causing urinary tract infection in urban community of meerut city, India.","authors":"Devanand Prakash, Ramchandra Sahai Saxena","doi":"10.1155/2013/749629","DOIUrl":"https://doi.org/10.1155/2013/749629","url":null,"abstract":"Urinary tract infection is one of the common infections in the Indian community. Distribution and susceptibility of UTI-causing pathogens change according to time and place. This study was conducted to determine the distribution and antimicrobial susceptibility of uropathogens in the Indian community as well as to determine the effect of gender and age on the etiology of bacterial uropathogens. Clean catch midstream urine samples were collected from 288 patients of the age ranging from 15 to ≥48 years. Antimicrobial susceptibility was performed on all isolated bacteria by Kirby Bauer's disc diffusion method. The multiple antibiotic resistance (MAR) index of each antibiotic was calculated. The UTI prevalence was 53.82% in patients; however, the prevalence was significantly higher in females than in males (females: 73.57%; males: 35.14%; P = 0.000). Females within the age group of 26-36 years and elderly males of ≥48 years showed higher prevalence of UTI. Gram negative bacteria (90.32%) were found in high prevalence than Gram positive (9.68%). Escherichia coli (42.58%) was the most prevalent gram negative isolate. Nitrofurantoin (78.71%) was found the most resistant drug among all uropathogens. Tested carbapenems were found the most susceptible drug against isolated uropathogens which showed 92.26% and 84.52% susceptibility, respectively. ","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2013 ","pages":"749629"},"PeriodicalIF":0.0,"publicationDate":"2013-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/749629","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31911742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 188

Receptors and Lethal Effect of Bacillus thuringiensis Insecticidal Crystal Proteins to the Anticarsia gemmatalis (Lepidoptera, Noctuidae). 苏云金芽孢杆菌杀虫晶体蛋白的受体及对夜蛾的杀伤作用。

ISRN Microbiology Pub Date : 2013-09-30 eCollection Date: 2013-01-01 DOI: 10.1155/2013/940284

Lidia Mariana Fiuza, Neiva Knaak, Rogério Fernando Pires da Silva, João Antônio Pêgas Henriques

引用次数: 13