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引用次数: 54
摘要
本工作的目的是从锯末、土壤和腐烂木材中分离出具有生产木聚糖酶和纤维素酶潜力的丝状真菌菌株。共分离到110株真菌。57种真菌从土壤样本中分离，32种从锯末中分离，19种从腐烂的木材中分离。木霉和曲霉的相对发生率最高，分别为42.6%和40.8%。木霉Fd18对木聚糖酶的蛋白质比活性最高，为1.30 U mg(-1);木霉F4对纤维素酶的蛋白质比活性最高，为1.23 U mg(-1)。分离得到的真菌具有合成水解酶的潜力。
Screening of fungi isolated from environmental samples for xylanase and cellulase production.
The aim of this work is to select filamentous fungal strains isolated from saw dust, soil, and decaying wood with the potential to produce xylanase and cellulase enzymes. A total of 110 fungi were isolated. Fifty-seven (57) of these fungi were isolated from soil samples, 32 from sawdust, and 19 from decaying wood. Trichoderma and Aspergillus had the highest relative occurrence of 42.6% and 40.8%, respectively. Trichoderma viride Fd18 showed the highest specific activity of 1.30 U mg(-1) protein for xylanase, while the highest cellulase activity of 1.23 U mg(-1) was shown by Trichoderma sp. F4. The isolated fungi demonstrated potential for synthesizing the hydrolytic enzymes.
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