Journal de mycologie medicale最新文献

{"title":"Cryptococcal meningoencephalitis in a patient on immunomodulatory drug: A case report","authors":"Terhi Juusola ,&nbsp;Elina Aho-Laukkanen ,&nbsp;Marko Rahkonen ,&nbsp;Vesa Mäki-Koivisto ,&nbsp;Ilkka S. Junttila","doi":"10.1016/j.mycmed.2024.101529","DOIUrl":"10.1016/j.mycmed.2024.101529","url":null,"abstract":"<div><h3>Background</h3><div><em>Cryptococcus neoformans</em> is an important fungal pathogen causing pneumonia and central nervous system infections mainly in immunocompromised hosts. Fingolimod is an immunomodulatory drug approved in the US and Europe for the treatment of multiple sclerosis.</div></div><div><h3>Case presentation</h3><div>We herein report a case of cryptococcal meningoencephalitis in a 46-year-old male with a history of fingolimod for five years. He suffered from a progressive headache and visual impairment. These symptoms led to a suspicion of a central nervous system infection and <em>C. neoformans</em> was identified with nucleic acid-based PCR method. Subsequently, appropriate treatment was initiated, and the patient recovered.</div></div><div><h3>Conclusions</h3><div>Our case underlines the importance of active diagnostic measures such as cerebrospinal fluid analysis in patients under fingolimod treatment with central nervous symptoms. While multiple sclerosis may cause headache and vision impairment, similar symptoms may be caused by central nervous system infections. It has been suggested that fingolimod may subject one to infections and this may occur even years after initiation of the treatment. For our case patient Cerebrospinal fluid sample combined with PCR-based identification provided a rapid diagnosis.</div></div>","PeriodicalId":14824,"journal":{"name":"Journal de mycologie medicale","volume":"35 1","pages":"Article 101529"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zingerone effect against Candida albicans growth and biofilm production","authors":"Sayali Chougule ,&nbsp;Sargun Basrani ,&nbsp;Tanjila Gavandi ,&nbsp;Shivani Patil ,&nbsp;Shivanand Yankanchi ,&nbsp;Ashwini Jadhav ,&nbsp;Sankunny Mohan Karuppayil","doi":"10.1016/j.mycmed.2024.101527","DOIUrl":"10.1016/j.mycmed.2024.101527","url":null,"abstract":"<div><h3>Background</h3><div>The increasing resistance of <em>Candida albicans</em> biofilms underscores the urgent need for effective antifungals. This study evaluated the efficacy of zingerone and elucidated its mode of action against <em>C. albicans</em> ATCC 90028 and clinical isolate C1.</div></div><div><h3>Experimental Procedure</h3><div>Minimum inhibitory concentrations (MICs) of zingerone were determined using CLSI methods against planktonic cells, biofilm formation, and yeast-to-hyphal transition. The mode of action was investigated through fluorescent microscopy, ergosterol assays, cell cycle analysis, and RT-PCR for gene expression.</div></div><div><h3>Key Results</h3><div>Zingerone inhibited planktonic growth and biofilm formation at in <em>C. albicans</em> ATCC 90028 and clinical isolate C1 at 2 mg/mL 4 mg/mL and 1 mg/mL and 2 mg/mL respectively. Treatment with the MIC concentration caused significant cell cycle arrest at the G0/G1 phase, halting proliferation in both the strains. Propidium iodide Staining revealed compromised membrane integrity in both the strains. Also, acridine orange and ethidium bromide dual staining showed increased dead cell proportions in <em>C. albicans</em> ATCC 90028. RT-PCR studies showed downregulation of <em>BCY1, PDE2, EFG1</em>, and upregulation of negative regulators <em>NRG1, TUP1</em> disrupting growth and virulence pathways. Zingerone induced elevated reactive oxygen species (ROS) levels, triggering apoptosis, evidenced by DNA fragmentation and upregulation of apoptotic markers. It also inhibited ergosterol synthesis in a concentration-dependent manner, crucial for membrane integrity. Importantly, zingerone exhibited minimal hemolytic activity. In an <em>in vivo</em> silkworm model, zingerone demonstrated significant antifungal efficacy, protecting silkworms from infection. It also modulated stress response genes, highlighting its multifaceted action.</div></div><div><h3>Conclusions</h3><div><em>In vitro</em> and <em>in vivo</em> findings confirm the potent antifungal efficacy of zingerone against <em>C. albicans</em> ATCC 90028 and clinical isolate C1, suggesting its promising potential as a therapeutic agent that warrants further exploration.</div></div>","PeriodicalId":14824,"journal":{"name":"Journal de mycologie medicale","volume":"35 1","pages":"Article 101527"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ibrexafungerp for the treatment of vulvovaginal candidiasis: A systematic review and meta-analysis of randomized placebo-controlled trials","authors":"Chia Siang Kow ,&nbsp;Dinesh Sangarran Ramachandram ,&nbsp;Syed Shahzad Hasan ,&nbsp;Kaeshaelya Thiruchelvam","doi":"10.1016/j.mycmed.2025.101534","DOIUrl":"10.1016/j.mycmed.2025.101534","url":null,"abstract":"<div><h3>Introduction</h3><div>Vulvovaginal candidiasis (VVC) is a prevalent fungal infection affecting millions of women globally, primarily caused by <em>Candida</em> species, most notably <em>Candida albicans</em>. Ibrexafungerp emerges as a promising candidate in the treatment arsenal against VVC, presenting a novel approach to combating this prevalent fungal infection.</div></div><div><h3>Methods</h3><div>A systematic literature search was conducted across major databases, including PubMed, EMBASE, and the Cochrane Library, to identify relevant randomized controlled trials (RCTs) evaluating the efficacy and safety of ibrexafungerp in the treatment of VVC. Following rigorous methodology, data extraction, risk of bias assessment using Cochrane's RoB 2 tool, and meta-analysis were conducted.</div></div><div><h3>Results</h3><div>Four RCTs were included in the analyses. The ibrexafungerp regimen utilized across the studies were 300 mg administered twice daily for one day. Meta-analysis revealed that ibrexafungerp was associated with significantly higher clinical cure rates compared to placebo in patients with VVC (pooled odds ratio (OR) 2.32; 95 % confidence interval (CI) 1.80 to 2.98). Complete symptom resolution was achieved in a greater proportion of participants receiving ibrexafungerp (pooled OR 2.76; 95 % CI 1.62 to 4.71). Analysis of treatment-emergent adverse events revealed a significant higher incidence of at least one treatment-emergent adverse event with ibrexafungerp compared to placebo (pooled OR 2.83; 95 % CI 2.06 to 3.88).</div></div><div><h3>Conclusion</h3><div>This study provides robust support for the efficacy of ibrexafungerp in the treatment of VVC. While the safety profile of ibrexafungerp appears favorable with mostly mild adverse events reported, decision-making in the clinical context should be guided by individual patient factors.</div></div>","PeriodicalId":14824,"journal":{"name":"Journal de mycologie medicale","volume":"35 1","pages":"Article 101534"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disseminated histoplasmosis in a 4-month-old infant presenting with prolonged fever and pancytopenia: A case report","authors":"Brady Floyd ,&nbsp;Farrah Gaston ,&nbsp;Reem Saadoon ,&nbsp;Nadia Shaikh ,&nbsp;Mustafa Bakir","doi":"10.1016/j.mycmed.2025.101532","DOIUrl":"10.1016/j.mycmed.2025.101532","url":null,"abstract":"<div><h3>Background</h3><div>Histoplasmosis is the most prevalent endemic mycosis in the United States, typically affecting immunocompromised individuals. Diagnosis of histoplasmosis in immunocompetent patients is rare, particularly among young infants, with only a few cases reported.</div></div><div><h3>Case presentation</h3><div>We present a 4-month-old female with a history of prematurity who initially presented with 11 days of fever. She visited her local emergency department, where she was diagnosed with Rhino/enterovirus bronchiolitis. Pancytopenia was noted at that time, attributed to viral bone marrow suppression. Persistent fever and pancytopenia led to her transfer to our hospital for further evaluation and treatment. On admission, she exhibited hepatosplenomegaly, pancytopenia, elevated procalcitonin, and lactate dehydrogenase levels. Additionally, T cell deficiency was observed. Initially, there was concern for hemophagocytic lymphohistiocytosis; however, bone marrow biopsy and aspirate confirmed T cell deficiency. As fever persisted on day 19, further investigations were conducted, including a positive beta-D-glucan assay. Subsequent urine and serum <em>Histoplasma</em> antigen tests were positive, with metagenomic sequencing confirming the diagnosis of histoplasmosis. Treatment comprised one week of amphotericin B followed by three months of oral itraconazole. Resolution of fever, pancytopenia, T cell deficiency, and hepatosplenomegaly occurred, and the patient has shown no signs of recurrence to date.</div></div><div><h3>Conclusions</h3><div>This case serves as a reminder to clinicians regarding the necessity of considering disseminated histoplasmosis in young infants who present with fever of unknown origin, pancytopenia, and hepatosplenomegaly, despite its rarity. Failure to do so can lead to fatal outcomes.</div></div>","PeriodicalId":14824,"journal":{"name":"Journal de mycologie medicale","volume":"35 1","pages":"Article 101532"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledging our reviewers","authors":"","doi":"10.1016/S1156-5233(25)00010-1","DOIUrl":"10.1016/S1156-5233(25)00010-1","url":null,"abstract":"","PeriodicalId":14824,"journal":{"name":"Journal de mycologie medicale","volume":"35 1","pages":"Article 101541"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of tinea capitis among schoolchildren in Mahajanga, northern Madagascar: An epidemio-clinical survey using conventional, proteomic and molecular approaches","authors":"Nambininiavo Marianne Ranorohasimanana ,&nbsp;Mohammad Akhoundi ,&nbsp;Alexandra Dorleans ,&nbsp;Emma Benamari ,&nbsp;Sedera Radoniaina Rakotondrasoa ,&nbsp;Dina Haribenja Rasoavololona ,&nbsp;Masy Nasandratra Razafindrakotosoa ,&nbsp;Arezki Izri ,&nbsp;Lala Rasoamialy-Soa Razanakolona ,&nbsp;Sophie Brun","doi":"10.1016/j.mycmed.2024.101528","DOIUrl":"10.1016/j.mycmed.2024.101528","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to determine the prevalence, clinical, and epidemiological features of tinea capitis (TC) in schoolchildren in Mahajanga city, north-west Madagascar, to identify the etiological dermatophyte species by morphological, proteomic and molecular approaches and ultimately to analyze the risk factors promoting TC in the studied region.</div></div><div><h3>Methods</h3><div>A survey was conducted in a randomly selected primary school. Symptomatic schoolchildren with signs resembling TC were sampled by scraping and sterile swabbing after examination of the scalp with a Wood's lamp. The collected specimens were then analyzed by a combination of conventional, proteomic, and molecular examinations</div></div><div><h3>Results</h3><div>The results of this study revealed a prevalence of TC of 8.7 % (39/449). Boys were much more affected than girls (M/<em>F</em> = 3.3). A little more than half (53.8 %) of TC cases exhibited large (&gt;2 cm) alopecic patches. Pruritus was the most frequent clinical sign in 74.4 % of cases. The incriminated species identified by morphology and confirmed by MALDI-TOF mass spectrometry and ITS sequencing were exclusively <em>Microsporum audouinii</em>. Playing football, running and contact with animals were statistically associated with TC. Inversely, the use of soap and the application of styling oil were protective against TC.</div></div><div><h3>Conclusion</h3><div>In this first investigation carried out in Mahajanga city, the prevalence of TC was 8.7 % and <em>M. audouinii</em> was the only responsible agent identified by three methods. Molecular approach allowed identification of the dermatophyte species in the specimens with inconclusive culture but positive direct examination. The risk factors such as playing football, running and contact with animals were significantly associated with TC.</div></div>","PeriodicalId":14824,"journal":{"name":"Journal de mycologie medicale","volume":"35 1","pages":"Article 101528"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bloodstream infection in a neonatal intensive care unit: Epidemiology, Antifungal susceptibility and new drug delivered strategies","authors":"Matheus Ribeiro ,&nbsp;Luciana da Silva Ruiz ,&nbsp;Rinaldo Ferreira Gandra ,&nbsp;Rubiana Mara Mainardes ,&nbsp;Carine Teles Sangaleti Miyahara ,&nbsp;Débora Moreira ,&nbsp;Maurício Domingues-Ferreira ,&nbsp;Diniz Pereira Leite Junior ,&nbsp;Claudete Rodrigues Paula ,&nbsp;Marcos Ereno Auler","doi":"10.1016/j.mycmed.2025.101535","DOIUrl":"10.1016/j.mycmed.2025.101535","url":null,"abstract":"<div><div>Bloodstream infection in neonates is a complicated disease and presents a major challenge both in diagnosis and in therapeutic intervention. The focus of the present study was to investigate the incidence, the species distribution and the risk factors associated with mortality of bloodstream infections in a neonatal intensive care unit (NICU) and evaluating the antifungal susceptibility of traditional antifungal drugs and three nanoparticle-based drug delivery systems based on nanoparticles. A total of 458 patients were evaluated, and 9.38 % were confirmed to have bloodstream infections through laboratory tests. The death rate was higher among neonates with fungal infections (66.7 %) compared to those with bacterial infections (5.4 %). Severe health conditions contributed to the increased mortality rate, especially gestational age <em>&lt;</em>28 weeks and weight <em>&lt;</em>1.000 g. Coagulase-negative staphylococci were the major pathogens (64.9 %) considering cases of neonatal sepsis. <em>Candida albicans</em> was the predominant causative agent among neonates with fungemia, although non-<em>albicans</em> species led to the highest mortality rates. <em>In vitro</em> antifungal activity evidenced resistance of <em>C. tropicalis</em> to fluconazole and voriconazole. Three nanoparticles were evaluated: chitosan-coated PLGA containing Amphotericin B, zein containing voriconazole and PLA containing voriconazole, and results were considered promising. The present findings demonstrate the importance of constant epidemiological surveillance in a NICU and the severity of fungal infection in neonates. The results suggest the potential of nanotechnology as an alternative in the treatment of fungal infection.</div></div>","PeriodicalId":14824,"journal":{"name":"Journal de mycologie medicale","volume":"35 1","pages":"Article 101535"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An unexpected guest: First report of Tintelnotia destructans as an agent of maxillary fungus ball","authors":"Jacques Sevestre ,&nbsp;Justin Michel ,&nbsp;Romain Appay ,&nbsp;Stéphane Ranque ,&nbsp;Thomas Radulesco ,&nbsp;Carole Cassagne","doi":"10.1016/j.mycmed.2025.101533","DOIUrl":"10.1016/j.mycmed.2025.101533","url":null,"abstract":"<div><div>Fungal rhinosinusal infections comprise several nosological entities, including sinus fungus ball. Diagnosis of sinus fungus ball relies on patient interrogation and clinical and paraclinical findings. Mold species commonly involved include <em>Aspergillus</em>, as well as dematiaceous fungi. Nevertheless, thanks to improvements in fungal culture and species level identification in clinical settings, new species are continuously described as agents in rhinosinusal infections. We present the first report of <em>Tintelnotia destructans</em> causing maxillary sinus fungus ball in a female patient.</div></div>","PeriodicalId":14824,"journal":{"name":"Journal de mycologie medicale","volume":"35 1","pages":"Article 101533"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143038978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory potency of active metabolites from different polarities of Datura Stramonium seed extracts: GC-MS analysis, biological evaluations, and molecular docking studies","authors":"K. Hjouji ,&nbsp;A. El Barnossi ,&nbsp;M. Er-rajy ,&nbsp;I. Atemni ,&nbsp;A. Grenha ,&nbsp;M. Yagoubi ,&nbsp;T. Ainane ,&nbsp;M. Taleb ,&nbsp;Z. Rais","doi":"10.1016/j.mycmed.2024.101521","DOIUrl":"10.1016/j.mycmed.2024.101521","url":null,"abstract":"<div><div><em>Datura Stramonium</em> is a well-known and important medicinal plant that is widely used in various medical systems to treat conditions such as asthma, diabetes, and inflammatory diseases. The aim of this study was to prepare extracts of <em>D. stramonium</em> seeds in different solvent polarities for assessing phytochemical potential, in vitro biological activities, and molecular docking studies. Phytochemical screening was conducted to determine the phytochemical composition, while GC-MS analysis was used to identify secondary metabolites of <em>D. Stramonium</em>. The seed extracts were molecularly docked to evaluate bioactive and antioxidant activity. The minimum inhibitory concentration (MIC) of the extracts against bacteria such as <em>S. aureus, E. coli, Bacillus subtilis</em>, and <em>Proteus mirabilis</em> was determined<em>.</em> Antifungal activity was also tested against fungi including <em>Aspergillus flavus, Fusarium oxysporum, Aspergillus niger</em>, and <em>Candida albicans</em>. The results of phytochemical screening indicated that the primary plant constituents in all extracts of different polarities are alkaloids, flavonoids, tannins, mucilage, sterols, heterosides, triterpenoids, and cardiac glycosides. Most molecules in the hexane, ether, and chloroform extracts consist of fatty acids, sterols, glycosides, triterpenoids, alkaloids, and phenolic compounds, as revealed by GC-MS analysis. Conversely, extracts from polar solvents like methanol, ethanol, and water are abundant in alkaloids. In vitro antibacterial and antifungal activities demonstrated that ether, methanol, and ethanol extracts were more effective than inhibiting the four bacterial strains compared to the antibiotics <em>Oxacillin</em> and <em>Cefuroxime</em>. The ether and methanol extracts exhibited better zones of inhibition and significant MIC values against <em>A. niger</em> and <em>C. albicans</em> compared to the control and fluconazole. The free radical inhibition (DPPH) (24.92 ± 5.31 μg/mL) for the ethanol extract indicates <em>Datura's</em> antioxidant capacity. It is believed that the main phytochemicals are responsible for the enhanced antioxidant activity observed in other studies. The docking study revealed that the bioactive compounds linoleic acid and atropine formed better hydrogen bonding interactions with proteins than pi-alkyl and alkyl bonds.</div></div>","PeriodicalId":14824,"journal":{"name":"Journal de mycologie medicale","volume":"35 1","pages":"Article 101521"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laboratory practices for the diagnosis and management of mucormycosis in France, 2024","authors":"Laurence Millon ,&nbsp;Françoise Botterel ,&nbsp;Julie Bonhomme ,&nbsp;Stéphane Valot ,&nbsp;Philippe Poirier ,&nbsp;Marie-Fleur Durieux ,&nbsp;Jeanne Bigot ,&nbsp;Guillaume Desoubeaux ,&nbsp;Adélaïde Chesnais ,&nbsp;Florent Morio ,&nbsp;Marc Pihet ,&nbsp;Kévin Brunet ,&nbsp;Anne-Pauline Bellanger ,&nbsp;Sébastien Imbert ,&nbsp;Gilles Nevez ,&nbsp;Solène Le Gal ,&nbsp;Nathalie Bourgeois ,&nbsp;Anne Debourgogne ,&nbsp;Marjorie Cornu ,&nbsp;Florence Persat ,&nbsp;Eric Dannaoui","doi":"10.1016/j.mycmed.2024.101520","DOIUrl":"10.1016/j.mycmed.2024.101520","url":null,"abstract":"<div><div>This study investigates the diagnostic practices for mucormycosis among 30 French University Hospital mycology laboratories, in 2024. All laboratories perform both direct examination and culture, with fluorescent brighteners being the most commonly used method for direct examination. While 77 % of the participating laboratories routinely identify Mucorales to the species level, with 70 % having adopted Mucorales-specific quantitative PCR, primarily for the diagnosis of invasive fungal infections. Antifungal susceptibility testing practices varied between centers, with 36.7 % of laboratories consistently performing these tests, primarily using gradient concentration strips. Amphotericin B, posaconazole, and isavuconazole were the most frequently tested antifungals. These findings highlight variations in laboratory practices and emphasize the importance of establishing uniform diagnostic and susceptibility testing methods to optimize mucormycosis management.</div></div>","PeriodicalId":14824,"journal":{"name":"Journal de mycologie medicale","volume":"34 4","pages":"Article 101520"},"PeriodicalIF":2.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}