Japanese Journal of Oral Biology最新文献

{"title":"Immunohistochemical Demonstration of Cathepsins B and L in the Periodontal Ligament (PDL) of the Rat Molar","authors":"M. Takeyama, K. Irie, Hiroaki Nakamura, E. Kominami, K. Hanada, H. Ozawa, S. Ejiri","doi":"10.2330/JORALBIOSCI1965.43.194","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.194","url":null,"abstract":"","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"31 1","pages":"194-202"},"PeriodicalIF":0.0,"publicationDate":"2001-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90799176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent Microscopic Analysis of Mandibula Surrounding a Pair of Titanium Implants as Anchorage for Orthodontic Tooth Movement in Adult Male Beagle Dogs","authors":"Y. Ohtani, T. Morohashi, Sigeru Saitoh, Masami Ohmae, Y. Shibasaki, S. Yamada","doi":"10.2330/JORALBIOSCI1965.43.156","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.156","url":null,"abstract":"チタンインプラント (IPL) が歯の矯正移動の固定源として有用であるかについて, 4頭のビーグル成犬を用いて検索を行った。下顎第2, 第3前臼歯相当部位に植立した2本のIPLを鋳造冠で連結し, 第4前臼歯との間にアーチワイヤーを用いて200gの側方力を負荷した。反対側には矯正力を負荷しなかった。矯正力負荷から2週間ごとにテトラサイクリン (TC) を皮下投与した。24-32週間の飼育終了後, 両側でIPLの移動は観察されなかった。反射電子像をもとにIPL矢状断面の観察を行った後, IPL先端から連続横断切片を作製し, IPL近遠心に設定された測定部位における石灰化領域およびTC標識で囲まれた領域の割合を算出した。反射電子像では矯正力の有無によるインプラント周囲の骨の状態に顕著な差はみられなかった。石灰化領域は約80%, TC標識領域は約35%で, 両側間に有意な差はなかった。IPLが移動せずかつIPL周囲の石灰化が矯正力の有無にかかわらず起こっていたという結果は, IPLが矯正による歯の移動の固定源として有用であることを示唆した。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"26 1","pages":"156-165"},"PeriodicalIF":0.0,"publicationDate":"2001-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74447834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytoskeletal Components Related to the Transport of Fuzzy Coated Vesicles and Secretory Granules in Acinar Cells of the Rat Submandibular Gland by Quick-frozen Freeze-substitution Fixation Method","authors":"M. Odajima","doi":"10.2330/JORALBIOSCI1965.43.1","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.1","url":null,"abstract":"Cytoskeletal components concerned with the intracellular transport of the fuzzy coated vesicles from the Golgi apparatus to the immature secretory granules, and the secretory granules toward the plasma membrane in the acinar cells of the rat submandibular gland were investigated using the quick-frozen freeze-substitution fixation method. It was observed that actin-like filamentous structures (4.2nm) connected fuzzy coated vesicles with intermediate filaments (9.2nm) in the vicinity of the Golgi area. It was also demonstrated that mature secretory granules were connected to the microtubules by actin-like filamentous structures and rod-like structures.The rod-like structure was composed of two globular structures and a filamentous structure. These observations suggest that actin-like filamentous structures, intermediate filaments, rod-like structures and microtubules were involved in the transport of fuzzy coated vesicles and secretory granules.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"38 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2001-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90742600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relative Growth of Molar Sizes in Three Species of Arvicolinae, Eothenomys andersoni, Eothenomys smithii and Microtus montebelli.","authors":"E. Sakai, Y. Uematsu","doi":"10.2330/JORALBIOSCI1965.43.43","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.43","url":null,"abstract":"In this paper, the mesiodistal and buccolingual crown diameters of the upper and lower molars in three Arvicolinae species , Eothenomys andersoni, Eothenomys smithii and Microtus montebelli were measured and their relative growth patterns relative to each condylobasal length were studied from the perspective of infraspecific relative growth and compared among the three species. The mesiodistal and buccolingual crown diameters of each upper and lower molar in all three species continue growing with the growth of condylobasal length. Comparing the growth potentials among molars, in each upper and lower molar relative to the condylobasal length the growth potential of the third molar was higher than that of the first and second molars except for the buccolingual crown diameters of the upper and lower molars in E . smithii. The growth potentials of mesiodistal crown diameters were higher than those of buccolingual crown diameters of all molars in E. andersoni, of the upper and lower third molars in E . smithii and of the upper third molar in M. montebelli. Therefore, the forms of these molars became relatively more slender as their condylobasal lengths increased. It can be surmised that the characteristics in relative growth of the upper and lower molars correspond to the characteristics in eating habits (herbivore) and the mandibular movement of the three Arvicolinae species. Comparing growth potentials among the three species , there was no fixed tendency among the relative growth coefficients (a). 抄録:ハ タネ ズ ミ亜科 に属 す ヤチネ ズ ミ,ス ミスネズ ミお よびハ タネズ ミの上 ・下顎大 臼歯 の近遠 心径 お よび 頬舌径 を計 測 し,頭 蓋 骨 基底 全長 に対 す る相対成 長様 相 を種 内相対 成長 の立場 か ら検 討 し,さ らにそれ ら を種 間 で比較 した。3種 の上 ・下 顎各 大臼歯 の近遠 心径 お よび頬舌径 は,頭 蓋骨 基底 全長 の成 長 に伴 って成長 を続 ける。 歯 種別 に成 長能 を比較 す る と,ス ミス ネズ ミの上 ・下 顎大 臼歯 の頬 舌径 を除 き,第3大 臼歯 で第1,第2大 臼歯 よ り成長 能が 高 い。 近遠 心径 の成長 能 は,ヤ チ ネズ ミの全 大 臼歯,ス ミスネ ズ ミの上 ・下顎第3大 臼歯 お よびハ タ ネズ ミの上顎 第3大 臼歯 で頬舌径 の それ よ り大 きい。 したが っ て,こ れ らの大 臼歯 は頭蓋骨 基底 全長 の成長 が進 むに従 い,相 対的 によ り細長 い形 態 を とる。 これ らの結 果 は,3種 の食性(食 植性)お よび下 顎運 動 と強 く結 び つ い てい る こ とを示 唆 して いる。種 間 で成 長能 を比較 す る と,相 対 成長 係数 αの値 に一 定の傾 向 は認 め られ な い 。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"4 1","pages":"43-59"},"PeriodicalIF":0.0,"publicationDate":"2001-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87418829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a Low Calcium Diet on the Levels of Protein Kinases Activity in Rat Parotid Glands","authors":"Pao‐Li Wang, S. Shirasu, Y. Azuma, Y. Tachi, H. Shimpuku, M. Shinohara, M. Daito, K. Ohura","doi":"10.2330/JORALBIOSCI1965.43.89","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.89","url":null,"abstract":"The adult human body contains approximately 1kg of calcium (Ca), 99% of which is stored in bones and teeth as hydroxyapatite crystals . Although the Ca in bone appears to be in a static condition , it can be mobilized into the blood when necessary. The mobilization of Ca plays an important physiological role in many processes including hormone secretion , muscle constriction, and blood coagulation. Previous studies have shown that Ca also plays an important role in bone tissue formation, and that a diet deficient in this element adversely affects the growth and development of the jaw bone1). Therefore, it is natural to suspect that consuming a Ca-deficient diet will lead to dysfunction in the oral cavity. Although extensive studies on the effect of Ca on bones have been reported, relatively little is known about the effect of a protein-deficient diet2) or Cadeficient diet3,4) on salivary gland function. The proteins in acinar cells undergo three processes, synthesis, packaging, and storage and release. Each process is regulated by phosphorylation, a protein tyrosine kinase (PTK) and cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA)5-7). Therefore, cAMP stimulates the transcription of genes encoding salivary proteins. cAMP also stimulates post-translational modification such as glycosylation. Finally, cAMP stimulates exocytosis. Thus, an increase in the level of cAMP within a cell stimulates every step involved in protein secretion. In addition to PKA, acinar cells contain numerous other protein kinases including protein kinase C (PKC), that are involved in different aspects of protein synthesis and release6,7).","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"10 1","pages":"89-93"},"PeriodicalIF":0.0,"publicationDate":"2001-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82559898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ectopic Mineralization of Hen Leg Tendon","authors":"T. Ono, T. Nemoto","doi":"10.2330/JORALBIOSCI1965.43.34","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.34","url":null,"abstract":"生後1年以上のニワトリの足の腱には, 石灰化物の沈着がみられる。さらに, 電子顕微鏡観察によれば, すでに生後100日以上の個体においてその徴候が認められる。本研究では, ニワトリ足腱の異所性石灰化部位の無機成分と有機マトリックス成分をプラズマ重合膜法によりオスミウムコートし, その走査電子顕微鏡観察を行った。その結果, コラーゲンマトリックスと石灰化物のそれぞれに, 新規の構造物を見いだした。第1に, 有機成分を溶解した後には長径が1.86±0.38μm (平均±標準偏差), 短径が1.05±0.16μm (平均±標準偏差) の楕円形の石灰化顆粒がみられ, それらはさらに融合して多数の細長いロッド状の構造を形成していた。さらに透過電顕観察では, 石灰化顆粒の表面には特徴的な67nmの周期的な縞模様が検出され, 本構造が生体内ではコラーゲン線維と接して存在していることが明らかとなった。一方, 脱灰処理後の腱のコラーゲン線維には, 2.8±0.38μmの周期的な波状構造が観察された。しかも, その一端のみが波状を呈する領域が観察され, その場合, 波状部分は必ず石灰化側に, 非波状部分は非石灰化側に位置していた。これらの結果は, 波状のコラーゲン線維構造が顆粒状の石灰化物生成を誘導している可能性を示唆している。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"6 1","pages":"34-42"},"PeriodicalIF":0.0,"publicationDate":"2001-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87218415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrophoretic Analysis of Alkaline Phosphatase in Rat Incisor Pulps","authors":"Toshiyuki Toen, S. Hashimoto","doi":"10.2330/JORALBIOSCI1965.43.17","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.17","url":null,"abstract":"ラット切歯歯髄組織中のアルカリ性ホスファターゼ (ALP) 活性は, マウスおよびハムスターと同程度であり, ウサギ, ウシ, ブタに比べて約10倍高かった。ラット歯髄組織から抽出したALPを, 0.1% Nonidet P-40を用いたポリアクリルアミドゲル電気泳動 (native-PAGE) あるいはラウリル硫酸ナトリウムを用いた非還元のポリアクリルアミドゲル電気泳動 (SDS-PAGE) にかけると, 酵素はnative-PAGEのゲル中ではALP-N1とALP-N2に, SDS-PAGEのゲル中では130kと155kの2つに分かれた。ALP-N1とN2は, 2次元電気泳動上で130と155kにそれぞれ一致した。粗抽出ALPを単に37℃で加温するだけで, ALP-N1 (130k) はALP-N2 (155k) に変化した。このALPの変化はALP結合性タンパク質によるのではなく, ALPの抽出分画中のグリコシルホスファチジルイノシトール特異的ホスホリパーゼD (GPI-PLD) によって特異的に生じることが明らかになった。精製したALP-N1は, ALP-N2と同様の等電点 (4.3) と, ALP-N2抗体に対し同程度の結合親和性を有していた。これに対して, 脂肪酸 (C14-C18) はALP-N1中にのみガスクロマトグラフィで検出され, ALP-N2およびホスファチジルイノシトール特異的ホスホリパーゼCで処理したALP-N1中には脂肪酸は認められなかった。ALP-N1に結合したSDS量は, 脂肪酸のないALP-N2に比べて有意に多かった。これらの結果は, SDS-PAGEの泳動ゲル中をALP-N1 (130k) がALP-N2 (155k) より速く移動することを示唆している。本研究は, ラット歯髄組織のALPは分子量77kのホモダイマーであり, ALPを抽出するとき血液由来のGPI-PLDにより脂肪酸を有するものと消失したものの2種類の酵素分子が生じることを明らかにした。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"447 1","pages":"17-33"},"PeriodicalIF":0.0,"publicationDate":"2001-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76720815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic Understanding of the Maturation of Developing Enamel: Plausible Interactions among Crystals, Matrix Proteins and Proteases","authors":"Masamitsu Yamazaki, Kaori Sato, T. Aoba","doi":"10.2330/JORALBIOSCI1965.43.60","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.60","url":null,"abstract":"本研究では, ブタの基質形成期エナメル質から抽出された基質タンパク, 合成ハイドロキシアパタイト, タンパク分解酵素としてトリプシンを使用して, エナメル溶液相を擬した実験溶液中でのアメロジェニンの酵素分解と, 結晶・タンパク・分解酵素が共存した反応系での結晶沈殿の制御機構について検討した。アメロジェニンの酵素分解過程の解析には, C末端エピトープを特異的に認識する2種類のペプチド抗体を使用した。溶液内においてアメロジェニン分子間で会合体が形成された場合に, トリプシンは会合体表面に位置する親水性のC末端領域を最初に切断することが碓かめられた。生理的な溶液条件下で結晶・タンパクを共存させると, アメロジェニンは会合体を形成しながら結晶表面に集積した。その結果, C末端の親水性領域への分解酵素の接近が阻害され, アメロジェニンの低分子化が遅延された。また, 結晶沈殿が誘起される過飽和溶液においては, 新たな結晶表面へのタンパク吸着が継続することから, 分解酵素によるアメロジェニンの低分子化は抑制された。以上の結果から, 酵素分解によるアメロジェニンの低分子化は, エナメル質の石灰化 (結晶成長の制御) と成熟化 (基質脱却) に直結した重要な現象であり, この低分子化現象には分解酵素と基質 (エナメルタンパク) との相互作用のみならず, 結晶・溶液組成が相互に関連しながら影響を及ぼしていると結論された。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"31 1","pages":"60-71"},"PeriodicalIF":0.0,"publicationDate":"2001-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76127247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scannning Electron Microscopic Study of the Insertion of the Human Stylohyoid Muscle into the Hyoid Bone","authors":"H. Saito, I. Itoh","doi":"10.2330/JORALBIOSCI1965.43.8","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.8","url":null,"abstract":"舌骨は嚥下や発声において複雑な運動を示す。茎突舌骨筋は舌骨を後上方に引き上げる筋であるが, 舌骨に付着する部位はこれまで明確にされていなかった。SEMを用いて観察した結果, 茎突舌骨筋は正中部を除く舌骨体下面に広く付着していることが明らかになった。この筋の付着部に, 厚さ0.5-1mm, 長さ10-17mmの線維軟骨塊が舌骨体の下面に沿って認められた。茎突舌骨筋の腱線維束は線維軟骨に入る前に, ほかの舌骨付着筋と錯綜していた。また線維軟骨塊の内部においても同様の錯綜が認められた。これらの錯綜は互いの筋が結合を強めあうことを示していると考えられた。このように線維軟骨塊に多くの筋が入り込むことは, 嚥下運動などにおいて, 筋で支持されている舌骨がその位置を変える運動を円滑にしている。また嚥下運動において, 舌骨は同時に回転運動を行うことがすでに知られている。多くの筋が線維軟骨塊に入り込むことは, この線維軟骨塊を固定することを可能にしている。したがって, 舌骨は線維軟骨塊を軸として, オトガイ舌骨筋や甲状舌骨筋により前後に回旋すると考えられた。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"32 1","pages":"8-16"},"PeriodicalIF":0.0,"publicationDate":"2001-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85544736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex Difference in Expression of Metallothionein mRNA in Mouse Liver after Low Dose Cadmium Injection","authors":"T. Fujioka, N. Sogawa, C. Sogawa, N. Oda, H. Furuta","doi":"10.2330/JORALBIOSCI1965.43.83","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.83","url":null,"abstract":"Environmental pollution caused by cadmium (Cd) has been an object of public concern for considerable time. Among heavy metals, both Cd and mercury are well known to be severe toxic to living tissue. Interestingly, it has been suggested that there are sex differences in Cd effects1-4). For example, blood pressure in male rats was elevated by administration of Cd, but not in females2). Itai-Itai disease, that has been considered a chronic symptom of Cd poisoning, was reported in old women, especially5). However, there is no consensus whether Cd effects are more severe in males or females, so a sex difference in the effect of Cd has remained obscure. Since Margoshes, et al.6) reported that low molecular weight proteins from horse kidney cortex contained abundant Cd and Kagi, et al.7) identified these proteins as metallothionein (MT), there have been many discussions concerning the functions of MT. According to reports suggesting that MT is induced in animal tissues by Cd administration and noting a positive correlation between dose-dependent increases in hepatic MT and Cd LD50, the detoxification of heavy metal has been considered one of the main functions of MT8). Therefore, an investigation of differences in MT synthesis by Cd in male and female animals should be of considerable interest for evaluating sex differences in the influences of Cd. In this study, we investigated MT mRNA expressions in the livers of male and female mice after Cd injections using the RT-PCR method.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"26 1","pages":"83-88"},"PeriodicalIF":0.0,"publicationDate":"2001-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84968361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}