Japanese journal of medical science & biology最新文献_第4页

Rapid and highly sensitive enzyme immunoassay for quantitative determination of tetrodotoxin. 快速、高灵敏度的河豚毒素定量测定酶免疫分析法。

Japanese journal of medical science & biology Pub Date : 1997-06-01 DOI: 10.7883/yoken1952.50.133

K Kawatsu, Y Hamano, T Yoda, Y Terano, T Shibata

{"title":"Rapid and highly sensitive enzyme immunoassay for quantitative determination of tetrodotoxin.","authors":"K Kawatsu, Y Hamano, T Yoda, Y Terano, T Shibata","doi":"10.7883/yoken1952.50.133","DOIUrl":"https://doi.org/10.7883/yoken1952.50.133","url":null,"abstract":"A monoclonal antibody against tetrodotoxin (TTX) was obtained from Balb/c mice immunized with TTX-bovine serum albumin (BSA) conjugate. The monoclonal antibody was highly specific for TTX and had no cross-reaction to tetrodonic acid, which is a TTX derivative, or gonyautoxins, although a minor cross-reaction to anhydro-tetrodotoxin was observed. The monoclonal antibody neutralized the lethal activity of TTX. By using the monoclonal antibody, a rapid and highly sensitive competitive enzyme immunoassay (EIA) for quantitative analysis of TTX was developed. By the competitive EIA system, TTX can be determined quantitatively in about 30 min (90 min are required if the time for preparation of the solid-phase antigen was included), and the working range for quantitative analysis of TTX was 2-100 ng/ml. In recovery tests and examinations of TTX samples, results of the mouse bioassay and EIA analyses correlated well (r = 0.987). Moreover, it was demonstrated that low concentrations of TTX, which could not be detected by the mouse bioassay, could be determined quantitatively by the competitive EIA.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"50 3","pages":"133-50"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7883/yoken1952.50.133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20478381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 79

Entomological and rodent surveillance in plague-suspected areas during September 1994 and thereafter. 1994年9月及以后在疑似鼠疫地区进行昆虫学和啮齿动物监测。

Japanese journal of medical science & biology Pub Date : 1997-06-01 DOI: 10.7883/yoken1952.50.97

K Kumar, S Jamil-Ur-Rahman, S K Sharma, K S Gill, R Katyal, R Kaur, T G Thomas, K Barua

{"title":"Entomological and rodent surveillance in plague-suspected areas during September 1994 and thereafter.","authors":"K Kumar, S Jamil-Ur-Rahman, S K Sharma, K S Gill, R Katyal, R Kaur, T G Thomas, K Barua","doi":"10.7883/yoken1952.50.97","DOIUrl":"https://doi.org/10.7883/yoken1952.50.97","url":null,"abstract":"Studies carried out in the states of Maharashtra, Gujarat, Uttar Pradesh and Union Territory of Delhi after the bubonic plague outbreak during 1994 revealed the presence of seven species of rodents, viz. Rattus rattus, R. norvegicus, Mus. musculus, Tatera indica, Suncus murinus, Bandicoota bengalensis and B. indica. The flea species encountered were Xenopsylla cheopis and X. astia. The X. cheopis and X. astia index recorded in different areas of Beed district of Maharashtra; Surat, Vadodra and Baruch districts in Gujarat and Varanasi district in Uttar Pradesh and their implications have been discussed. Insecticide suspectibility tests carried out against DDT, dieldrin, malathion and deltamethrin with X. cheopis collected from Maharashtra, Delhi and Varanasi revealed that this vector species is resistant to DDT and dieldrin but susceptible to malathion and deltamethrin. The prevalence and distribution of rodents species, high cheopis index and prevalence of Tatera indica just at the door steps of houses in village Mamla of Beed district provides highly congenital conditions for the intermingling of wild and domestic rodents and transfer of flea population from wild to domestic rodents and vice-versa. These conditions were found to be highly supportive for bubonic plague transmission in the district. The presence of Yersinia pestis antibodies in Rattus rattus collected from Beed, Surat and Varanasi areas are also indicative of bubonic plague in Beed and Varanasi and pneumonic plague in Surat during 1994.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"50 3","pages":"97-111"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7883/yoken1952.50.97","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20478463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Molecular typing of Vibrio vulnificus isolates by random amplified polymorphic DNA (RAPD) analysis. 随机扩增多态性DNA (RAPD)分析创伤弧菌分离株的分子分型。

Japanese journal of medical science & biology Pub Date : 1997-06-01 DOI: 10.7883/yoken1952.50.113

D W Ryang, S W Cho, M G Shin, J H Shin, S P Suh

{"title":"Molecular typing of Vibrio vulnificus isolates by random amplified polymorphic DNA (RAPD) analysis.","authors":"D W Ryang, S W Cho, M G Shin, J H Shin, S P Suh","doi":"10.7883/yoken1952.50.113","DOIUrl":"https://doi.org/10.7883/yoken1952.50.113","url":null,"abstract":"This study was undertaken to determine molecular types and genetic similarity among V. vulnificus isolates by RAPD analysis. We compared these results with serotypes of V. vulnificus. Ninety-seven V. vulnificus strains including 69 strains from Chonnam University Hospital (CUH; Kwangju, Korea), 13 from Wonkwang University Hospital (WUH; Iksan, Korea), 13 from the Japanese National Institute of Health (JNIH) and two reference strains (ATCC 33815 and ATCC 27562) were analyzed. Four molecular types comprising all the strains were obtained by RAPD analysis. Type I was the most common (60/95) and included 58 strains from CUH. Type I showed a further subdivision into seven subtypes. Type II (23/95) composed of 11 strains from CUH, nine from WUH, three from JNIH and two reference strains. Six type III strains comprised four WUH strains and two JNIH strains. All six strains of type IV were from JNIH. The range of genetic similarity values among V. vulnificus isolates was 0.24 to 1.00. The serotypes of 95 strains were 04 (84.2%), 014 (3.2%), 01 (2.1%), 013 (2.1%), and R (2.1%). The most common 04 serotype strains were distributed among types I (60 strains), II (23 strains), III and IV (six strains). Although the V. vulnificus isolates showed a wide range of genetic similarity values, RAPD analysis could separate V. vulnificus strains into four molecular types, and the isolates from the same hospitals tended to belong to the same molecular type. There was no specific correlation between molecular types and serotypes of V. vulnificus.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"50 3","pages":"113-21"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20478379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Effects of thymosin alpha-1 on erythrocyte lipid levels and erythrocyte membrane (Na(+)-K+)-ATPase activity in experimental hypercholesterolemia. 胸腺酶α -1对实验性高胆固醇血症红细胞脂质水平和红细胞膜(Na(+)-K+)- atp酶活性的影响。

Japanese journal of medical science & biology Pub Date : 1997-04-01

C Gökkuşu, E Ademoğlu, H Oz, U M Türkoğlu

引用次数: 0

A comparison of young and aged populations for the diphtheria and tetanus antitoxin titers in Japan. 日本年轻人和老年人白喉和破伤风抗毒素滴度的比较。

Japanese journal of medical science & biology Pub Date : 1997-04-01 DOI: 10.7883/yoken1952.50.87

M Takahashi, T Komiya, T Fukuda, Y Nagaoka, R Ishii, F Goshima, H Arimoto, H Kaku, N Nakajima, T Goshima, T Katoh

引用次数: 21

Distribution of Rift Valley fever among cattle in Zambia. 裂谷热在赞比亚牛群中的分布。

Japanese journal of medical science & biology Pub Date : 1997-04-01 DOI: 10.7883/yoken1952.50.73

K L Samui, S Inoue, A S Mweene, A M Nambota, J E Mlangwa, P Chilonda, M Onuma, C Morita

引用次数: 22

Discrimination of Listeria monocytogenes strains of serotype 4b by restriction enzyme analysis of chromosomal DNA. 染色体DNA限制性内切酶分析鉴定血清型4b单核细胞增生李斯特菌。

Japanese journal of medical science & biology Pub Date : 1997-04-01 DOI: 10.7883/yoken1952.50.63

A Saito, T Sawada, Y Tokumaru, R Hondo

引用次数: 1

Effects of thymosin alpha-1 on erythrocyte lipid levels and erythrocyte membrane (Na(+)-K+)-ATPase activity in experimental hypercholesterolemia. 胸腺酶α -1对实验性高胆固醇血症红细胞脂质水平和红细胞膜(Na(+)-K+)- atp酶活性的影响。

Japanese journal of medical science & biology Pub Date : 1997-04-01 DOI: 10.7883/YOKEN1952.50.45

C. Gökkuşu, E. Ademoglu, H. Oz, U. Türkoğlu

引用次数: 1

Evaluation of culture, histological examination, serology and the rapid urease test for diagnosis of Helicobacter pylori in patients with dyspepsia in Bangladesh. 孟加拉国消化不良患者幽门螺杆菌培养、组织学检查、血清学和快速脲酶试验诊断的评价

Japanese journal of medical science & biology Pub Date : 1997-04-01 DOI: 10.7883/yoken1952.50.55

M G Morshed, F Jinnah, M S Islam, M A Rumi, S Ahmed, M M Ahmed, M Sadeque, M F Chowdhury

引用次数: 8

The effects of cyclosporin A on the polykaryocyte formation induced by measles virus in a monkey kidney cell line. 环孢素A对麻疹病毒诱导的猴肾细胞系多核细胞形成的影响。

Japanese journal of medical science & biology Pub Date : 1997-04-01 DOI: 10.7883/yoken1952.50.79

E Watari, K Yokomuro

引用次数: 1