Japanese journal of medical science & biology最新文献_第10页

ADENOVIRUS SURVEILLANCE, 1982-1993, JAPAN 腺病毒监测，1982-1993年，日本

Japanese journal of medical science & biology Pub Date : 1995-01-01 DOI: 10.7883/YOKEN1952.48.199

S. Yamadera, K. Yamashita, M. Akatsuka, N. Kato, M. Hashido, S. Inouye, S. Yamazaki

引用次数: 23

Potential use of specific human and chicken antibodies for detection of Hanganutziu-Deicher antigen(s) in sera of cancer patients. 人类和鸡特异性抗体在癌症患者血清中检测汉格努茨乌-德歇尔抗原的潜在应用。

Japanese journal of medical science & biology Pub Date : 1994-10-01 DOI: 10.7883/yoken1952.47.253

C J Mukuria, A Noguchi, E Suzuki, M Naiki

引用次数: 5

Production of a factor inhibiting tumor-cell migration in patients with gastric and breast cancer. 胃癌和乳腺癌患者肿瘤细胞迁移抑制因子的产生。

Japanese journal of medical science & biology Pub Date : 1994-10-01 DOI: 10.7883/yoken1952.47.265

I T Raichev, R Borissova

{"title":"Production of a factor inhibiting tumor-cell migration in patients with gastric and breast cancer.","authors":"I T Raichev, R Borissova","doi":"10.7883/yoken1952.47.265","DOIUrl":"https://doi.org/10.7883/yoken1952.47.265","url":null,"abstract":"Production of a factor with a biological activity to inhibit the in vitro tumor-cell migration (TCM) from peripheral blood E rosette-forming cells (ERFC), CD4+ and CD8+ T cells in patients with gastric and breast carcinoma was investigated. The cells were stimulated for 2 or 24 hr with allogeneic gastric or breast cancer extracts in samples of cell suspensions. A microculture system at an initial cell concentration from 2,500 cells to 1 cell per well was used. Feeder cells, PHA, IL-2-containing supernatant and cancer extract were added to each well. Ehrlich ascites tumor cells were employed in the migration-inhibition assay. ERFC and CD4+ T cells produced in the culture supernatants a factor inhibiting TCM, when these cells were stimulated with cancer extracts, but not with extracts of benign tissue. Stimulated CD8+ T cells did not produce such a factor. The production of the factor inhibiting TCM in the microculture system was also significantly correlated with the type of cells in the wells, particularly with ERFC and CD4+ T cells, but not with CD8+ T cells (r = 0.94, p < 0.001). It could be suggested that this factor probably took part in in vivo blockading the migration of tumor cells in small cancer foci.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"47 5-6","pages":"265-80"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18740611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Growth inhibition of herpes simplex virus-1 in the cells expressing abundant 2.0-kilobase latency-associated transcripts. 单纯疱疹病毒-1在表达丰富的2.0千碱基潜伏期相关转录物的细胞中的生长抑制

Japanese journal of medical science & biology Pub Date : 1994-10-01 DOI: 10.7883/yoken1952.47.281

K Hara, M Toba, Y Yogo, F Taguchi

引用次数: 0

Cure with cisplatin (II) or murine malaria infection and in vitro inhibition of a chloroquine-resistant Plasmodium falciparum isolate. 治疗顺铂(II)或小鼠疟疾感染和体外抑制氯喹耐药恶性疟原虫分离物。

Japanese journal of medical science & biology Pub Date : 1994-10-01 DOI: 10.7883/yoken1952.47.241

L Nair, V K Bhasin

{"title":"Cure with cisplatin (II) or murine malaria infection and in vitro inhibition of a chloroquine-resistant Plasmodium falciparum isolate.","authors":"L Nair, V K Bhasin","doi":"10.7883/yoken1952.47.241","DOIUrl":"https://doi.org/10.7883/yoken1952.47.241","url":null,"abstract":"Antiplasmodium properties of cisplatin [cis-platinum (II) diamine dichloride], a neoplastic drug, have been assessed in in vivo and in vitro model systems of malarial parasite. A well-tolerated dose of 6 mg/kg body weight of the compound cured the mice infected with Plasmodium berghei and the amount of cisplatin required for in vitro inhibition (IC50) of a chloroquine-resistant Plasmodium falciparum isolate was smaller than either chloroquine or quinine. The minimum inhibitory concentration (MIC) needed to prevent the in vitro multiplication of asexual blood parasites was 30 ng/ml. Late ring and trophozoite stages of the erythrocytic cycle were the most susceptible, whereas schizont and early ring stages were the least sensitive to the toxic effect of cisplatin. Multiple smaller doses were more effective in curing malaria in mice than a single large dose. In a few of the mice treated with a single intraperitoneal large dose of 6 mg/kg body weight, there was a delay in appearance of parasitemia but most of them recovered completely but slowly. This compound exerts its toxicity mainly by randomly damaging and cross-linking DNA strands as shown by Southern hybridization with a synthetic oligonucleotide probe, which is a repeat sequence in the falciparum genome. The report clearly demonstrates the antimalarial potentials of this compound and suggests a closer evaluation of this and other related compounds, specially in combination with antimalarial drugs to probe their synergistic properties.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"47 5-6","pages":"241-52"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7883/yoken1952.47.241","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18740607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Epidemics of aseptic meningitis due to echovirus 30 in Japan. A report of the National Epidemiological Surveillance of Infectious Agents in Japan. 埃可病毒30在日本引起的无菌性脑膜炎流行。日本全国传染病流行病学监测报告。

Japanese journal of medical science & biology Pub Date : 1994-08-01 DOI: 10.7883/yoken1952.47.221

K Yamashita, K Miyamura, S Yamadera, N Kato, M Akatsuka, M Hashido, S Inouye, S Yamazaki

{"title":"Epidemics of aseptic meningitis due to echovirus 30 in Japan. A report of the National Epidemiological Surveillance of Infectious Agents in Japan.","authors":"K Yamashita, K Miyamura, S Yamadera, N Kato, M Akatsuka, M Hashido, S Inouye, S Yamazaki","doi":"10.7883/yoken1952.47.221","DOIUrl":"https://doi.org/10.7883/yoken1952.47.221","url":null,"abstract":"Two rages of epidemic of aseptic meningitis (AM) due to echovirus 30 (E30) in Japan were analyzed with respect to two sources of information, AM incidence and E30 isolation, both gathered through the National Epidemiological Surveillance of Infectious Diseases. The first E30 epidemic spread throughout Japan in 1983 and ceased within the year. The second epidemic, starting in 1989, continued for the three successive years, and in the last year, 1991, the total E30 reports numbered 4,061, the largest number of a single virus type ever reported. Although the epidemic showed temporal and geographical shift and lasted for one or two years in some areas, most laboratories reported the largest number of E30 isolation in 1991. Among E30-yielding cases with clinical information during 1982-1992, the associating frequency with AM was as high as 82.5%. Other central nervous system involvements such as encephalitis, myelitis, encephalomyelitis and/or paralysis were reported in 36 E30-yielding cases and their monthly and age distributions were different from those of AM cases. The proportion of such disease among E30-yielding cases (0.60%) was close to that of other enteroviruses (0.56%). During the epidemics, E30 was isolated more frequently from cerebrospinal fluid than was E4 or E9 which prevailed coincidentally. E30 was most frequently isolated from cases of 4-7 years of age, sharing the common characteristic pattern of age distribution with other enteroviral meningitis. E30-yielding cases, however, involved a large number of older age groups than those of other enterovirus infections, and this tendency was the most pronounced in the first epidemic year, 1983. The contribution of these E30 epidemics on the yearly trend of clinically reported AM incidence and on the shift of its age distribution was also analyzed.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"47 4","pages":"221-39"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7883/yoken1952.47.221","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18717567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Trans-suppression of gene expression by hepatitis C viral core protein. 丙型肝炎病毒核心蛋白反式抑制基因表达。

Japanese journal of medical science & biology Pub Date : 1994-08-01 DOI: 10.7883/yoken1952.47.211

D W Kim, R Suzuki, T Harada, I Saito, T Miyamura

引用次数: 65

Heterogeneity in lysosomal fusion with phagocytic vesicles and cell membrane in non-phagocytosing guinea-pig polymorphonuclear leukocyte. 非吞噬豚鼠多形核白细胞溶酶体与吞噬囊泡和细胞膜融合的异质性。

Japanese journal of medical science & biology Pub Date : 1994-08-01 DOI: 10.7883/yoken1952.47.179

F Amano, R Hashida, D Mizuno

{"title":"Heterogeneity in lysosomal fusion with phagocytic vesicles and cell membrane in non-phagocytosing guinea-pig polymorphonuclear leukocyte.","authors":"F Amano, R Hashida, D Mizuno","doi":"10.7883/yoken1952.47.179","DOIUrl":"https://doi.org/10.7883/yoken1952.47.179","url":null,"abstract":"The fusion of lysosomes and other granules with phagocytic vesicles (endo-fusion) and cell membrane (exocytosis) was simultaneously examined in non-phagocytosing guinea-pig polymorphonuclear leukocytes (PMNs). beta-Glucuronidase as a typical lysosomal enzyme, acid phosphatase as another lysosomal enzyme, and alkaline phosphatase as a specific granule enzyme were assayed. PMNs released these three enzymes in the presence of serum but the extents of exocytosis differed considerably: release of beta-glucuronidase, alkaline phosphatase and acid phosphatase was 6.9, 4.3 and 3.3%, respectively. Acid phosphatase was released even in the absence of serum, whereas the other two enzymes were not. These three enzymes showed also different responses in endofusion: the preformed phagocytic vesicles fused with the granules containing beta-glucuronidase and acid phosphatase, but scarcely fused with those containing alkaline phosphatase, although all these enzymes were recovered in increasing amounts in the phagolysosome fraction when the cells were allowed to phagocytose continuously. These results suggest that fusion of these three types of granules with phagocytic vesicles (endo-fusion) and plasma membrane (exocytosis) is heterogeneous and regulated by different mechanisms in guinea-pig PMNs.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"47 4","pages":"179-93"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18717564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Class II-restricted presentation of an immunoglobulin heavy-chain-gene product by a gene-transfected B-cell line. ii类限制免疫球蛋白重链基因产物由基因转染的b细胞系呈递。

Japanese journal of medical science & biology Pub Date : 1994-08-01 DOI: 10.7883/yoken1952.47.195

Y Matsuura, S Onishi, Y Yamamoto, T Taniguchi, S Obana, H Yamamoto

{"title":"Class II-restricted presentation of an immunoglobulin heavy-chain-gene product by a gene-transfected B-cell line.","authors":"Y Matsuura, S Onishi, Y Yamamoto, T Taniguchi, S Obana, H Yamamoto","doi":"10.7883/yoken1952.47.195","DOIUrl":"https://doi.org/10.7883/yoken1952.47.195","url":null,"abstract":"The presentation of an antigen endogenously processed by B lymphocytes was investigated. The expression plasmid vectors, harboring genomic rearranged V genes from two monoclonal B cells and genomic mu-constant region gene, were constructed. Two B-cell lines, the MOPC104E myeloma mu-heavy chain expressing AMB line and the control hybridoma mu-heavy chain expressing AHB line, were established by gene transfection into A20.2J B lymphoma cell line. The cloned transfectant cell lines expressed surface and cytoplasmic IgM. Radioimmunoprecipitation analysis of surface IgM revealed that both cell lines used transfected mu-heavy chain and host-derived kappa-light chain. The T-cell line, MRT-2, specific for the MOPC104E protein, proliferated on AME B cell lines but not on control AHB-cell lines. MRT-2 proliferation was inhibited by anti-I-Ed,k,p,r but not by anti-I-Ad monoclonal antibody. Although the AME-transfectant lines secrete IgM into the culture medium, double chamber-type culture-experiments revealed that MRT-2 proliferation is not mediated by the uptake of secreted IgM. The results suggest that B cells process and present their own immunoglobulin heavy-chain V-region peptides to T cells in the context of MHC class-II molecules.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"47 4","pages":"195-210"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18717565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic comparison between archetypal and disease-associated JC virus isolates in Japan. 日本典型与疾病相关JC病毒分离株的系统发育比较。

Japanese journal of medical science & biology Pub Date : 1994-06-01 DOI: 10.7883/yoken1952.47.167

K Kato, J Guo, F Taguchi, O Daimaru, M Tajima, H Haibara, J Matsuda, M Sumiya, Y Yogo

{"title":"Phylogenetic comparison between archetypal and disease-associated JC virus isolates in Japan.","authors":"K Kato, J Guo, F Taguchi, O Daimaru, M Tajima, H Haibara, J Matsuda, M Sumiya, Y Yogo","doi":"10.7883/yoken1952.47.167","DOIUrl":"https://doi.org/10.7883/yoken1952.47.167","url":null,"abstract":"We examined the phylogenetical correlation between two types of JC virus (JCV) isolates, archetypes derived from the urine of nonimmunocompromised individuals and PML-types derived from the brain of patients with progressive multifocal leukoencephalopathy (PML) in Japan. A phylogenetic tree was constructed for eight JCV isolates, five PML-types obtained in this and previous studies and three representative archetypes, from DNA sequence data on the VP1 (major capsid protein) gene. The eight isolates were divided into two major groups, named subtypes MY and CY after the representative archetypal isolates. Four of five PML-type isolates belonged to subtype MY, and the other one to subtype CY. Isolates belonging to subtype MY were further divided into two groups; one group containing archetype MY and three PML-types and the other one containing archetype YI and a PML-type. These findings, together with those in our previous study that correlated various JCV isolates in the world provide evidence for the hypothesis that JCVs associated with PML may have been generated from archetypal JCVs persisting in the patients.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"47 3","pages":"167-78"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18823441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21