Molecular typing of Vibrio vulnificus isolates by random amplified polymorphic DNA (RAPD) analysis.

Abstract

This study was undertaken to determine molecular types and genetic similarity among V. vulnificus isolates by RAPD analysis. We compared these results with serotypes of V. vulnificus. Ninety-seven V. vulnificus strains including 69 strains from Chonnam University Hospital (CUH; Kwangju, Korea), 13 from Wonkwang University Hospital (WUH; Iksan, Korea), 13 from the Japanese National Institute of Health (JNIH) and two reference strains (ATCC 33815 and ATCC 27562) were analyzed. Four molecular types comprising all the strains were obtained by RAPD analysis. Type I was the most common (60/95) and included 58 strains from CUH. Type I showed a further subdivision into seven subtypes. Type II (23/95) composed of 11 strains from CUH, nine from WUH, three from JNIH and two reference strains. Six type III strains comprised four WUH strains and two JNIH strains. All six strains of type IV were from JNIH. The range of genetic similarity values among V. vulnificus isolates was 0.24 to 1.00. The serotypes of 95 strains were 04 (84.2%), 014 (3.2%), 01 (2.1%), 013 (2.1%), and R (2.1%). The most common 04 serotype strains were distributed among types I (60 strains), II (23 strains), III and IV (six strains). Although the V. vulnificus isolates showed a wide range of genetic similarity values, RAPD analysis could separate V. vulnificus strains into four molecular types, and the isolates from the same hospitals tended to belong to the same molecular type. There was no specific correlation between molecular types and serotypes of V. vulnificus.