Iranian Biomedical Journal最新文献

Dietary Conjugated Linoleic Acid Isomers Modify the Fatty Acid Composition of Liver and Adipose Tissues. sdafsdf。

Iranian Biomedical Journal Pub Date : 2025-08-04 DOI: 10.61186/ibj.4990

Guillermo Hernández Díaz, Julio C. Ramírez Nava, Isaac Aguirre Maldonaldo, Beatriz Agame Lagunes, Narvick L. Cortez Ríos, Sandra Hernandez Leyva, Hugo S. García, Alfonso Alexander Aguilera

{"title":"Dietary Conjugated Linoleic Acid Isomers Modify the Fatty Acid Composition of Liver and Adipose Tissues.","authors":"Guillermo Hernández Díaz, Julio C. Ramírez Nava, Isaac Aguirre Maldonaldo, Beatriz Agame Lagunes, Narvick L. Cortez Ríos, Sandra Hernandez Leyva, Hugo S. García, Alfonso Alexander Aguilera","doi":"10.61186/ibj.4990","DOIUrl":"10.61186/ibj.4990","url":null,"abstract":"ewrtr.Background: Conjugated linoleic acids are a group of isomers derived from linoleic acid, naturally present in beef, lamb, and dairy products. CLA has been reported to exert therapeutic effects on cancer, obesity, and diabetes. This study evaluated the effects of a mixture containing equal proportions of CLA isomers (c9,t11 and t10,c12) on IR and their incorporation into the plasma membranes of adipocytes and hepatocytes in SHRs.Methods: For eight weeks, one group of hypertensive rats received sunflower oil supplemented with CLA isomers, while the control group received only vegetable oil. Serum parameters were measured, and the fatty acid composition of plasma membranes was analyzed using gas chromatography.Results: Rats treated with CLA isomers showed a reduction in BW, IR, and hypertension. Additionally, there was a significant incorporation of CLA into the plasma membranes of adipocytes and hepatocytes. In contrast, the control rats displayed higher levels of n-6 PUFA and AA in their membranes, which promote the synthesis of eicosanoids and hypertensive prostaglandins. These findings were further supported by data mining analysis, which linked the results to the expression levels of genes encoding enzymes involved in the synthesis of PGE2, D2, and F2α from AA.Conclusion: The mixture of CLA isomers reduced BW, IR, and HTN in SHRs. These effects were associated with the incorporation of c9,t11 and t10,c12 isomers into the plasma membranes of adipocytes and hepatocytes.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transdermal Iontophoresis Insulin in Laboratory Animals: A Systematic Review. 实验动物经皮离子导入胰岛素：系统综述。

Iranian Biomedical Journal Pub Date : 2025-07-26 DOI: 10.61186/ibj.5127

Zeinab Ahmadpour Emshi, Mohammad Mohsen Roostayi, Aliyeh Daryabor, Sedigheh Sadat Naimi, Maryam Niajalili

引用次数: 0

Apyrase Protein (PsSP42) Expression in DNA Construct Transfected COS-7 Cells. Apyrase Protein （PsSP42）在DNA构建体转染COS-7细胞中的表达

Iranian Biomedical Journal Pub Date : 2025-06-25 DOI: 10.61186/ibj.5055

Samira Hosseinpour Jahednia, Hossein Rezvan, Alireza Nourian, Tahereh Taheri, Negar Seyed, Zahra Eghbali, Elham Gholami, Sima Rafati

{"title":"Apyrase Protein (PsSP42) Expression in DNA Construct Transfected COS-7 Cells.","authors":"Samira Hosseinpour Jahednia, Hossein Rezvan, Alireza Nourian, Tahereh Taheri, Negar Seyed, Zahra Eghbali, Elham Gholami, Sima Rafati","doi":"10.61186/ibj.5055","DOIUrl":"https://doi.org/10.61186/ibj.5055","url":null,"abstract":"Background: DNA vaccines offer dual humoral and cellular immunity, showing potential effectiveness against intracellular parasites, including Leishmania. However, challenges in delivering large DNA constructs has been already existed, which influence protein expression and immune efficacy. This study evaluated the expression of P. sergenti salivary protein PsSP42 in COS-7 cells. Confirming the expression of PsSP42 is crucial before its use as a DNA vaccine in preclinical experiments.Methods: The expression of the PsSP42 protein was achieved using two plasmids: the small, antibiotic-free plasmid NTC9385R and the conventional plasmid VR1020. Both recombinant vectors were transfected into COS-7 cells using electroporation and PEI-mediated transfection methods. Ni-NTA beads were utilized to enrich proteins from the supernatants collected from transfected cells, and expression was confirmed by Western blotting.Results: Successful expression of the PsSP42 protein was affirmed from both VR1020-PsSP42 and NTC-PsSP42 constructs in the transfected COS-7 cells, regardless of the transfection method employed (PEI or electroporation). A 39.6 kDa band corresponding to the PsSP42 protein was detected, indicating its secretion into the supernatants of COS-7 cells transfected with both plasmids.Conclusion: Plasmids VR1020-PsSP42 and NTC-PsSP42 demonstrated similar protein expression levels in vitro, regardless of the transfection method used. Performing these evaluations is recommended to thoroughly assess construct expression levels before conducting in vivo studies.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoferritin-Functionalized with Wheat Germ Agglutinin and Loaded with Antibiotic for Targeting Bacteria. 用小麦胚芽凝集素功能化载铁蛋白并装载抗生素靶向细菌。

Iranian Biomedical Journal Pub Date : 2025-06-25 DOI: 10.61186/ibj.5039

Qristine G Gyurjyan, Gayane G Poghosyan, Vardan K Gasparyan

{"title":"Apoferritin-Functionalized with Wheat Germ Agglutinin and Loaded with Antibiotic for Targeting Bacteria.","authors":"Qristine G Gyurjyan, Gayane G Poghosyan, Vardan K Gasparyan","doi":"10.61186/ibj.5039","DOIUrl":"https://doi.org/10.61186/ibj.5039","url":null,"abstract":"Background: The selective delivery of drugs to their targets prevents their possible side effects; hence, the development of selective transport systems is considered extremely promising. In the present study, we aimed to develop a drug delivery system for targeting bacteria.Methods: Functionalization of apoferritin with WGA, as a bacteria-recognizing lectin, was conducted. Afterwards, the complex was loaded with ampicillin. The affinity of the conjugate to Gram-positive bacteria, B. subtilis, was evaluated by anisotropic AgNPs conjugated with this complex, and its interaction with the bacteria was also assessed.Results: The drug-delivery capabilities of the composite were evaluated. Results from the disk diffusion assay revealed that its bactericidal activity is 10-fold greater compared to free antibiotics. The effectiveness of the bactericidal effects of ampicillin-loaded ferritin was also confirmed in whole blood.Conclusion: Lectin-conjugated and ampicillin-loaded apoferritin can be considered as effective drug delivery systems for targeting bacteria.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145053608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Cancer Treatment: A Comprehensive Review of Active and Passive Drug Delivery Strategies. 优化癌症治疗：主动和被动给药策略的综合综述。

Iranian Biomedical Journal Pub Date : 2025-06-04 DOI: 10.61186/ibj.4960

Parastoo Tarighi, Seyedeh Mona Mousavi Esfahani, Ali Emamjomeh, Seyedeh Zohreh Mirjalili, Parastoo Mirzabeigi

引用次数: 0

Design and Assessment of the Immunogenic Potential of a Multi-Peptide Vaccine Construct Against Uropathogenic Escherichia coli-Associated Urinary Tract Infection. 针对尿路致病性大肠杆菌相关尿路感染的多肽疫苗结构的免疫原性潜力的设计和评估。

Iranian Biomedical Journal Pub Date : 2025-05-22 DOI: 10.61186/ibj.5149

Saeide Mirsharifi, Mehri Habibi, Touraj Rahimi, Fatemeh Foroohi, Mohammad Reza Asadi Karam

{"title":"Design and Assessment of the Immunogenic Potential of a Multi-Peptide Vaccine Construct Against Uropathogenic Escherichia coli-Associated Urinary Tract Infection.","authors":"Saeide Mirsharifi, Mehri Habibi, Touraj Rahimi, Fatemeh Foroohi, Mohammad Reza Asadi Karam","doi":"10.61186/ibj.5149","DOIUrl":"https://doi.org/10.61186/ibj.5149","url":null,"abstract":"Background: Urinary tract infection caused by UPEC is a common infectious disease. The growing frequency of antibiotic resistance highlights the need for alternative strategies, such as vaccines, to combat UTIs. This study aimed to evaluate the immunogenicity of a novel vaccine candidate targeting UPEC.Methods: Different bioinformatics servers were used to design a vaccine candidate composed of PapG II and FimH antigens from UPEC, along with the N- (1-173) and C-terminal (401-495) domains of FliC from S. typhimurium. The final construct was cloned into the pET28a vector, expressed, purified, and confirmed using SDS-PAGE and Western blotting. Mice were immunized with the recombinant protein, both with and without alum adjuvant, and antibody responses were measured using ELISA.Results: The final vaccine construct included one domain of PapG II (81 aa) and FimH (83 aa). The conserved domains of FliC were incorporated into the construct. SDS-PAGE and Western blot confirmed the purification of the protein, with a size of 53 kDa. Immunization of mice with PapG.FimH.FliC protein induced significantly higher levels of serum IgG, IgG isotypes, IgA, as well as mucosal IgA and IgG responses compared to the controls (p < 0.05). The addition of alum to the protein significantly enhanced serum IgG1 and IgA, as well as mucosal IgG, compared to the protein without alum (p < 0.05).Conclusion: The vaccine construct induced significant humoral responses in the mouse model, suggesting its potential as a promising candidate against UPEC. However, additional experimental analyses are required to validate the efficacy of the vaccine construct.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing Oral Films Based on Beeswax: Comparative Assessment of 3D Printing and Solvent Casting. 基于蜂蜡的口腔膜设计：3D打印与溶剂铸造的对比评价。

Iranian Biomedical Journal Pub Date : 2025-05-22 DOI: 10.61186/ibj.5098

Pegah Torabi, Shahab Bohlooli, Shadab Shahsavari, Leila Rezaei Shirmard

{"title":"Designing Oral Films Based on Beeswax: Comparative Assessment of 3D Printing and Solvent Casting.","authors":"Pegah Torabi, Shahab Bohlooli, Shadab Shahsavari, Leila Rezaei Shirmard","doi":"10.61186/ibj.5098","DOIUrl":"https://doi.org/10.61186/ibj.5098","url":null,"abstract":"Background: From the perspective of DDSs, OFs have received increased attention, mainly for pediatric and geriatric applications. Beeswax, a naturally derived and FDA-approved material, is often mixed with other polymers to enhance its mechanical properties. This study presented the first use of precisely controlled, solvent-free pressure-assisted micro-syringe printing to produce OFs.Methods: Solvent casting and pressure-assisted micro-syringe printing were employed to produce hybrid film structures composed of beeswax, PVA, borax, and HPMC, loaded with betamethasone as a model drug. The films were characterized based on their physical appearance, mechanical attributes, surface structure, and ultrastructure morphology via SEM, drug content, and in vitro drug release.Results: Films without the drug showed greater irregularities and roughness compared to the drug-loaded films. The physical properties of the formulations improved through 3D printing.Conclusion: By using 3D printing methods in pharmaceuticals, the treatment procedurewould be very acceptable to the patient and increases the patient's adherence to treatmentand is useful for personal drug delivery.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of rs4646287 Polymorphism with the Risk of Hepatitis B Virus Infection and the Progression of Hepatocellular Carcinoma in an Iranian Population. sdfsd。

Iranian Biomedical Journal Pub Date : 2025-05-21 DOI: 10.61186/ibj.4979

Hassan Akrami, Mohammad Reza Fattahi, Mastaneh Zeraatiannejad, Jamal Sarvari, Yousef Nikmanesh, Zahra Mansourabadi, Zahra Yazdani

{"title":"Association of rs4646287 Polymorphism with the Risk of Hepatitis B Virus Infection and the Progression of Hepatocellular Carcinoma in an Iranian Population.","authors":"Hassan Akrami, Mohammad Reza Fattahi, Mastaneh Zeraatiannejad, Jamal Sarvari, Yousef Nikmanesh, Zahra Mansourabadi, Zahra Yazdani","doi":"10.61186/ibj.4979","DOIUrl":"10.61186/ibj.4979","url":null,"abstract":"sadfsadfsdafsdf.Background: Hepatitis B virus is responsible for more than one million deaths annually, mainly due to HBV-related diseases and HCC. HBV enters hepatocytes and interacts with the NTCP. While several SNPs have been linked to HBV infection and HCC, further research is needed to clarify the precise role of SNPs. The relationship of the rs4646287 SNP with the risk of HBV infection and the progression of cirrhosis and HCC has been investigated in different populations. This study aimed to evaluate the association of the rs4646287 SNP with HBV infection, cirrhosis, and HCC in an Iranian population.Methods: The whole blood DNA was extracted from healthy individuals and patients with HBV, cirrhosis, and HCC. Primers for the C and T variants were designed using Primer1. The genotypes of the samples were identified using Tetra-ARMS PCR. The Tetra-ARMS PCR products were analyzed by electrophoresis on 2.5% agarose gels.Results: Individuals with the rs4646287 TT genotype exhibited a significantly reduced risk of developing cirrhosis and HCC compared to healthy controls. The TT genotype also showed a decreased correlation between the HBV group and those with cirrhosis and HCC.Conclusion: Our findings suggest that the rs4646287 TT genotype is associated with a lower risk of developing HBV-related diseases and HCC in an Iranian population.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Experimental Platforms to Assess Helicobacter pylori HopQ Interaction with Host CEACAM Molecules. 幽门螺杆菌HopQ与宿主CEACAM分子相互作用实验平台的建立

Iranian Biomedical Journal Pub Date : 2025-05-01 DOI: 10.61186/ibj.5029

Nazanin Shans, Maryam Esmaeili, Kimia Abraheh, Niloofar Asadi Hanjani, Maedeh Farrokhi, Negar Sardarpour, Yeganeh Talebkhan, Fatemeh Kazemi-Lomedasht, Esmat Mirabzadeh, Marjan Mohammadi

{"title":"Development of Experimental Platforms to Assess Helicobacter pylori HopQ Interaction with Host CEACAM Molecules.","authors":"Nazanin Shans, Maryam Esmaeili, Kimia Abraheh, Niloofar Asadi Hanjani, Maedeh Farrokhi, Negar Sardarpour, Yeganeh Talebkhan, Fatemeh Kazemi-Lomedasht, Esmat Mirabzadeh, Marjan Mohammadi","doi":"10.61186/ibj.5029","DOIUrl":"10.61186/ibj.5029","url":null,"abstract":"Background: Helicobacter pylori is an extracellular bacterium responsible for various gastrointestinal diseases, such as peptic ulcers and gastric cancer. It uses multiple mechanisms to colonize the harsh, acidic environment of the stomach and establish its pathogenic processes, mostly through CagA translocation. While cell surface integrin molecules were previously believed to be the main mediators anchoring H. pylori and facilitating this process, recent studies highlight the critical role of the interaction between the bacterial adhesin Helicobacter pylori outer membrane protein Q (HopQ) and host carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) in CagA translocation and subsequent pathogenic signaling.Methods: Recombinant proteins, including HopQ, HopQ-GFP (green fluorescent protein), HopQ-HRP (horseradish peroxidase), and recombinant N-terminal domain of human CEACAM1 (C1ND), were produced via gene cloning, expression, and purification techniques. Ligand-receptor interactions were evaluated using FACS analysis along with antigen- and cell-based ELISA assays.Results: In this study, we have developed antigen and cell-based platforms using recombinant fusion proteins (HopQ-GFP and HopQ-HRP) that effectively interact with recombinant C1ND, as well as various CEACAM molecules expressed on gastric cell lines (MKN45 and AGS).Conclusion: These assay platforms enable detailed investigation of the HopQ-CEACAM interaction and supports high-throughput screening of inhibitors, facilitating the identification of potential drugs or vaccine candidates targeting H. pylori infection.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 3","pages":"138-148"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12397998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Peptides and Bioactive Peptides on Acute Kidney Injury: A Review Study. 多肽和生物活性肽在急性肾损伤中的作用研究综述。

Iranian Biomedical Journal Pub Date : 2025-05-01 DOI: 10.61186/ibj.5000

Zeynab Mohamadi Yarijani, Houshang Najafi

引用次数: 0