Iranian Biomedical Journal最新文献_第2页

Evaluation of the Effects of Whey Protein and Moringa Oleifera Leaves Extract Mixture on Osseointegration in Rabbits. 评估乳清蛋白和油辣木叶提取物混合物对家兔骨结合的影响

Iranian Biomedical Journal Pub Date : 2024-03-01 DOI: 10.61186/ibj.4025

Nawar Bahjet Kamil, Nada M H Al-Ghaban

{"title":"Evaluation of the Effects of Whey Protein and Moringa Oleifera Leaves Extract Mixture on Osseointegration in Rabbits.","authors":"Nawar Bahjet Kamil, Nada M H Al-Ghaban","doi":"10.61186/ibj.4025","DOIUrl":"10.61186/ibj.4025","url":null,"abstract":"Background: Osteogenic, antioxidant and anti-inflammatory effects of Whey protein and M. oleifera gel prompted us to evaluate their role alone or in combination on osseointegration in rabbits.Methods: In this study, 24 titanium implants were inserted in the femurs of six rabbits. One implant was placed without treatment, and another one was coated with a mixture of whey protein and M. oleifera gel for each side. The animals were divided into two groups of 2- and 6-week intervals and evaluated using histopathological and immunohistochemical techniques.Results: Histological evaluation revealed a significant difference between the experimental and the control groups after two weeks in osteoblast and osteocyte counts. The experimental group had mature bone development after six weeks of implantation, while the control group had a woven bone. Immunohistochemical results showed that the experimental group, compared to the control group, exhibited early positive expression of osteoblast cells at two weeks after the experiment. Based on histopathological observations, the experimental group showed a tiny area of collagenous fiber in 6th week after the implantation.Conclusion: A mixture of whey protein and M. oleifera could accelerate osseointegration and healing processes.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"28 2&3","pages":"82-9"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11186610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141071065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of Wound Healing and Angiogenesis Using Mouse Embryo Fibroblasts Loaded in Decellularized Skin Scaffold. 利用负载在脱细胞皮肤支架中的小鼠胚胎成纤维细胞促进伤口愈合和血管生成

Iranian Biomedical Journal Pub Date : 2024-03-01 DOI: 10.61186/ibj.3971

Armaghan Gheytasvand, Hamed Bagheri, Shahram Pourbeyranvand, Mojdeh Salehnia

{"title":"Enhancement of Wound Healing and Angiogenesis Using Mouse Embryo Fibroblasts Loaded in Decellularized Skin Scaffold.","authors":"Armaghan Gheytasvand, Hamed Bagheri, Shahram Pourbeyranvand, Mojdeh Salehnia","doi":"10.61186/ibj.3971","DOIUrl":"10.61186/ibj.3971","url":null,"abstract":"Background: Synthetic and natural polymer scaffolds can be used to design wound dressing for repairing the damaged skin tissue. This study investigated acute wound healing process using a decellularized skin scaffold and mouse embryo fibroblast (MEF).Methods: Mouse skin fragments were decellularized and evaluated by DNA content, toxicity, H&E staining, Raman confocal microscopy, Masson’s trichrome staining, SEM, and biodegradation assays. The fragments were recellularized by the MEFs, and cell attachment and penetration were studied. De- and decellularized scaffolds were used wound dressings in mouse acute wound models as two experimental groups. Using morphological and immunohistochemical assessments, wound healing was evaluated and compared among the experimental and control groups.Results: DNA content of the decellularized tissue significantly reduced compared to the native control group (7% vs. 100%; p < 0.05). extracellular matrix components, e.g. collagen types I, III, and IV, elastin, and glycosaminoglycan, were well preserved in the decellularized group. The porosity and fiber arrangement in the stroma had a structure similar to normal skin tissue. A significant reduction in healing time was observed in the group treated with a decellularized scaffold. A thicker epidermis layer was observed in the recovered tissue in both experimental groups compared to the control group. Immunostaining showed a positive reaction for CD31 as an endothelial marker in both experimental groups, confirming new vascularization in these groups.Conclusion: Using MEFs with decellularized skin as a wound dressing increases the rate of wound healing and also the formation of new capillaries. This system could be beneficial for clinical applications in the field of tissue engineering.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"28 2&3","pages":"90-101"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11186609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141071057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyethylene Glycol -Mediated Exosome Isolation: A Method for Exosomal RNA Analysis. 聚乙二醇介导的外泌体分离：外泌体 RNA 分析方法。

Iranian Biomedical Journal Pub Date : 2024-03-01 DOI: 10.61186/ibj.4129

Abdulwahab Teflischi Gharavi, Azadeh Niknejad, Saeed Irian, Amirabbas Rahimi, Mona Salimi

{"title":"Polyethylene Glycol -Mediated Exosome Isolation: A Method for Exosomal RNA Analysis.","authors":"Abdulwahab Teflischi Gharavi, Azadeh Niknejad, Saeed Irian, Amirabbas Rahimi, Mona Salimi","doi":"10.61186/ibj.4129","DOIUrl":"10.61186/ibj.4129","url":null,"abstract":"Background: : Exosomal RNAs (ExoRNAs) offer valuable insights into their cellular origin. ExoRNA studies were faced with challenges in obtaining sufficient amounts of high-quality RNA. Herein, we aimed to compare three traditional exosome isolation methods to introduce an appropriate strategy to extract RNA from cancer-derived exosomes for further RNA analysis.Methods: Exosomes were isolated through ultracentrifugation, precipitation kit, and size exclusion column chromatography, and then characterized by dynamic light scattering and transmission electron microscopy, followed by extracting total RNA. The quality and quantity of the extracted RNAs were assessed by a NanoDrop and 2.5% agarose gel electrophoresis.Results: Extracted exosomes displayed a similar range of size and morphology. We found that polyethylene glycol-precipitation method resulted in a higher RNA yield with a 260/280 ratio of 1.9. The obtained exoRNA appeared as a smear in the agarose gel, indicative of small exoRNAs.Conclusion: We provide researchers a suitable approach to isolate exosomes based on yield and purity of exoRNA.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":" ","pages":"132-9"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11186611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140101598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Hydroxysteroid Dehydrogenase Type 1 As a Potential Bladder Tumor Marker. 鉴定作为潜在膀胱肿瘤标记物的 1 型羟类固醇脱氢酶

Iranian Biomedical Journal Pub Date : 2024-03-01 DOI: 10.61186/ibj.4068

Aida Albadawy, Mohammed Alqudaimi, Hanyue Cui, Xianghui Yan, Jing Sun, Ping Shi

{"title":"Identification of Hydroxysteroid Dehydrogenase Type 1 As a Potential Bladder Tumor Marker.","authors":"Aida Albadawy, Mohammed Alqudaimi, Hanyue Cui, Xianghui Yan, Jing Sun, Ping Shi","doi":"10.61186/ibj.4068","DOIUrl":"10.61186/ibj.4068","url":null,"abstract":"Background: The 17beta-hydroxysteroid dehydrogenase type 1 (HSD17B) family has been implicated in the prognosis and treatment prediction of various malignancies; however, its association with bladder cancer (BLCA) remains unclear. This study aimed to evaluate the potential of HSD17B1, as a prognostic biomarker, for the survival of patients with BLCA and to determine its effectiveness as a supplemental biomarker for BLCA.Methods: A series of bioinformatics techniques were applied to investigate the expression of HSD17B1 in different types of cancer and its potential association with the prognosis of BLCA patients using diverse databases. The UALCAN, Human Protein Atlas, cBioPortal, Metascape, GEPIA, MethSurv, and TIMER were employed to analyze expression differences, mutation status, enrichment analysis, overall survival, methylation, and immune-infiltrating cells. The real-time reverse transcription-PCR (qRT-PCR) was implemented to detect the messenger ribonucleic acid (mRNA) expression levels of HSD17B1 in vitro.Results: Elevated mRNA and protein levels of HSD17B1, surpassing normal levels, were observed in BLCA samples. In addition, the BLCA patients with higher mRNA expression level of HSD17B1 significantly reduced the overall survival. Also, several immune infiltrating cells, including mast cell resting CIBERSORT-ABS, have been identified as tumor-associated biomarker genes, with the potential to significantly influence the immunological environment. Finally, qRT-PCR analysis revealed a significant upregulation of HSD17B1 mRNA expression level in the cancer cells compared to the human 293T cells, which was consistent with the bioinformatics data.Conclusion: There is a strong correlation between the elevated HSD17B1 expression and positive prognosis in patients with BLCA. Therefore, HSD17B1 can be used as a prognostic biomarker in these patients.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"28 2&3","pages":"120-31"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11186615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumin Lowers the Accelerated Speed of Epileptogenesis by Traumatic Brain Injury. 姜黄素能降低创伤性脑损伤加速癫痫发生的速度

Iranian Biomedical Journal Pub Date : 2024-03-01 DOI: 10.61186/ibj.3978

Hanieh Jahi, Mansoureh Eslami, Mohammad Sayyah, Fariba Karimzadeh, Melika Alesheikh

{"title":"Curcumin Lowers the Accelerated Speed of Epileptogenesis by Traumatic Brain Injury.","authors":"Hanieh Jahi, Mansoureh Eslami, Mohammad Sayyah, Fariba Karimzadeh, Melika Alesheikh","doi":"10.61186/ibj.3978","DOIUrl":"10.61186/ibj.3978","url":null,"abstract":"Background: Traumatic brain injury or TBI can underlie epilepsy. Prevention of PTE has been of great interest to scientists. Given the antiepileptic, antioxidant and anti-inflammatory activities of curcumin, we examined whether this compound can affect epileptogenesis in rats after TBI.Methods: Curcumin was injected once a day for two weeks. TBI was induced in the temporal cortex of anesthetized rats using a controlled cortical impact device. One day after TBI, pentylenetetrazole (PTZ), 35 mg/kg, was injected i.p. every other day until manifestation of generalized seizures. The number of PTZ injections was then recorded. Moreover, the extent of cortical and hippocampal IL-1β and glial fibrillary acidic protein (GFAP) expression in the epileptic rats were measured by Western blot analysis.Results: Curcumin 50 and 150 mg/kg prevented the development of kindling, whereas TBI accelerated the rate of kindling. Curcumin 20 mg/kg prohibited kindling facilitation by TBI, and reduced the expression of IL-1β and GFAP induced by TBI.Conclusion: Curcumin can stop the acceleration of epileptogenesis after TBI in rats. Inhibiting hippocampal and cortical overexpression of IL-1β and GFAP seems to be involved in this activity.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":" ","pages":"113-9"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11186616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Monocyte Activation Test for Evaluating Recombinant Hepatitis B Vaccine: A Novel Approach for Pyrogen Assessment. 开发用于评估重组乙型肝炎疫苗的单核细胞活化试验：热原评估的新方法。

Iranian Biomedical Journal Pub Date : 2024-02-03 DOI: 10.61186/ibj.4100

Delaram Doroud, Zohre Eftekhari, Mojtaba Daneshi, Parisa Gheibi, Nazanin Jabbari, Maryam Khatami, Marzieh Hosseini

{"title":"Development of a Monocyte Activation Test for Evaluating Recombinant Hepatitis B Vaccine: A Novel Approach for Pyrogen Assessment.","authors":"Delaram Doroud, Zohre Eftekhari, Mojtaba Daneshi, Parisa Gheibi, Nazanin Jabbari, Maryam Khatami, Marzieh Hosseini","doi":"10.61186/ibj.4100","DOIUrl":"https://doi.org/10.61186/ibj.4100","url":null,"abstract":"Background: Injectable products, particularly human vaccines, must be free from fever-inducing agents and thoroughly tested for pyrogens as part of a quality control. Consequently, manufacturing facilities are required to conduct appropriate pyrogen tests per pharmacopeial standards. This study aimed to evaluate the reliability of the MAT in quantifying pyrogenic content in the recombinant hepatitis B vaccine.Methods: We assessed pyrogen activity in the API, formulated vaccine, and aluminum hydroxide by comparing the LAL, RPT, and MAT, measuring activity in RPU as per the European Pharmacopeia. Monocytes from healthy donors were isolated and identified via flow cytometry to measure the CD14+ marker frequency.Results: The study found that the pyrogenic concentration of LTA in the MAT was 50,000 ng/mL (5.19 EEU/mL). In contrast, the same concentration in the RPT was deemed non-pyrogenic based on rectal temperature assessments. The MAT showed sensitivity to the API and adjuvant, with a detection limit of 2.5 EU/mL for IL-6, outperforming the RPT, which had a detection limit of 5 EU/mL.Conclusion: A strong IL-6 response to both LPS and LTA stimulation was observed, indicating that IL-6 could serve as a valuable marker for pyrogen testing. The MAT appears to be an effective alternative to the RPT for assessing pyrogenicity, demonstrating commendable consistency and accuracy across various testing systems allowed by the Ph. Eur. General MAT Chapter, especially given the RPT's limitations in controlling pyrogenicity in injectable products.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocompatibility and Osseointegration of the Biomimetically Coated and Water-Soluble Eggshell Membrane Protein Cross-Linked Ti Alloy Screws. 生物仿生涂层和水溶性蛋壳膜蛋白交联钛合金螺钉的生物相容性和骨结合。

Iranian Biomedical Journal Pub Date : 2024-01-01 Epub Date: 2023-10-24 DOI: 10.61186/ibj.3939

Mehmet Bahadır İlik, Ercan Durmuş, İlhami Çelik

{"title":"Biocompatibility and Osseointegration of the Biomimetically Coated and Water-Soluble Eggshell Membrane Protein Cross-Linked Ti Alloy Screws.","authors":"Mehmet Bahadır İlik, Ercan Durmuş, İlhami Çelik","doi":"10.61186/ibj.3939","DOIUrl":"10.61186/ibj.3939","url":null,"abstract":"Background: The surface properties of dental and orthopedic implants are directly related to their osseointegration rate. Coating and/or modifying the implant surface might reduce the time of healing. In this study, we aimed to examine the effects of a hybrid surface consisting of a brushite surface coating and cross-linked water-soluble eggshell membrane protein on the osseointegration of titanium (Ti) screws under in vivo conditions.Methods: Twenty Ti alloy screws were implanted monocortically in anteromedial regions of New Zealand rabbit tibiae. Ten screws were untreated and used as controls. The remaining 10 screws were coated with calcium phosphate and following cross-linked with ostrich eggshell membrane protein. All rabbits were sacrificed six weeks after the surgery. Peri-screw tissues were evaluated by micro-computed tomography (µ-CT), histological and histomorphometrical methods.Results: The μ-CT assessments indicated that the experimental group had significantly higher mean bone surface area (BSA) and trabeculae number (TbN) than those of the control group (p ˂ 0.05). Bone surface area (BV), trabecular separation (TbSp), trabecular thickness (TbTh), and bone mineral density (BMD) scores of the control and experimental groups were quite similar (p > 0.05). The vascularization score of the experimental group was significantly higher than the control group (4.29 vs. 0.92%). No sign of the graft-versus-host reaction was observed.Conclusion: Our findings reveal that coating Ti alloy implants with calcium phosphate cross-linked with ostrich eggshell membrane protein increases the osseointegration of Ti alloy screws by increasing the bone surface area, number of trabeculae and vascularization in the implant site.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":" ","pages":"38-45"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10994639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140110273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumin Modulates NOX Gene Expression and ROS Production via P-Smad3C in TGF-β-Activated Hepatic Stellate Cells. 姜黄素通过 P-Smad3C 调节 TGF-β 激活的肝星状细胞中 NOX 基因的表达和 ROS 的产生

Iranian Biomedical Journal Pub Date : 2024-01-01 Epub Date: 2023-10-28 DOI: 10.61186/ibj.4005

Shahla Asadizade, Mahdi Hatami, Samaneh Salehipour Bavarsad, Benyamin Kabizade, Elham Shakerian, Mojtaba Rashidi

{"title":"Curcumin Modulates NOX Gene Expression and ROS Production via P-Smad3C in TGF-β-Activated Hepatic Stellate Cells.","authors":"Shahla Asadizade, Mahdi Hatami, Samaneh Salehipour Bavarsad, Benyamin Kabizade, Elham Shakerian, Mojtaba Rashidi","doi":"10.61186/ibj.4005","DOIUrl":"10.61186/ibj.4005","url":null,"abstract":"Background: Liver fibrosis, associated with hepatic stellate cells (HSCs), occurs when a healthy liver sustains damage, thereby impairing its function. NADPH oxidases (NOXs), specifically isoforms 1, 2, and 4, play a role in reactive oxygen species (ROS) production during hepatic injuries, resulting in fibrosis. Curcumin has shown strong potential in mitigating liver fibrosis. Our research aimed to investigate the effects of curcumin on lowering NOX and ROS levels. This compound was also studied for its effects on NOXs, ROS concentrations through the inhibition of Smad3 phosphorylation in transforming growth factor beta (TGF-β)-activated human HSCs.Methods: MTT assay investigated the cytotoxic effects of curcumin on HSCs. The cells were activated by exposure to TGF-β (2 ng/mL) for 24 hours. After activating, the cells were treated with curcumin at 25-150 μM concentrations. After administering curcumin to the cells, we employed RT-PCR and Western blot techniques to evaluate the related gene and protein expression levels. This evaluation was primarily focused on the mRNA expression levels of NOX1, NOX2, NOX4 and phosphorylated Smad3C.Results: The mRNA expression level of aforesaid NOXs as well as α-smooth muscle actin (α-SMA), collagen1-α, and ROS levels were significantly reduced following 100 μM curcumin treatment. Furthermore, curcumin significantly decreased the p-Smad3C protein level in TGF-β-activated cells, with fold changes of 3 and 2 observed at 75 and 100 μM, respectively.Conclusion: Curcumin decreased the levels of ROS and NOX, as well as the expression of α-SMA and collagen1-α. The primary mechanism for this reduction could be linked to the level of p-Smad3C. Hence, curcumin could serve as an effective therapeutic agent for liver fibrosis.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":" ","pages":"31-7"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10994637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140101596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lessons from COVID-19 Pandemic: A Successful Policy and Practice by Pasteur Institute of Iran. 从 COVID-19 大流行中吸取的经验教训：伊朗巴斯德研究所的成功政策与实践。

Iranian Biomedical Journal Pub Date : 2024-01-01 Epub Date: 2023-10-11 DOI: 10.61186/ibj.3964

Mona Sadat Larijani, Alireza Biglari, Rahim Sorouri, Mostafa Salehi-Vaziri, Delaram Doroud, Keyhan Azadmanesh, Fatemeh Fotouhi, Ehsan Mostafavi, Amitis Ramezani

引用次数: 0

Simvastatin-Loaded Nanoniosome Protects H9c2 Cells from Oxygen-Glucose Deprivation/ Reperfusion Injury by Downregulating Inflammation. 辛伐他汀载体纳米生物体通过抑制炎症保护H9c2细胞免受氧-葡萄糖剥夺/再灌注损伤的影响

Iranian Biomedical Journal Pub Date : 2024-01-01 Epub Date: 2023-09-30 DOI: 10.61186/ibj.3994

Maryam Naseroleslami, Mahdieh Mehrab Mohseni

{"title":"Simvastatin-Loaded Nanoniosome Protects H9c2 Cells from Oxygen-Glucose Deprivation/ Reperfusion Injury by Downregulating Inflammation.","authors":"Maryam Naseroleslami, Mahdieh Mehrab Mohseni","doi":"10.61186/ibj.3994","DOIUrl":"10.61186/ibj.3994","url":null,"abstract":"Background: Simvastatin (SIM) has anti-inflammatory and antioxidant properties against cardiac ischemia/reperfusion injury (I/RI). However, it suffers from low bioavailability and a short half-life. Nanoniosomes are novel drug delivery systems that may increase SIM effectiveness. The present research evaluates the impact of SIM-loaded nanoniosomes on the oxygen-glucose deprivation/reperfusion (OGD/R) injury model of H9c2 cells.Methods: Cells were seeded based on five groups: (1) control; (2) OGD/R; (3) OGD/R receiving SIM; (4) OGD/R receiving nanoniosomes; and (5) OGD/R receiving SIM loaded nanoniosomes. OGD/R injury of the H9c2 cells was treated with SIM or SIM loaded nanoniosomes. Cell viability, two inflammatory factors, necroptosis factors, along with HMGB1 and Nrf2 gene expressions were assessed.Results: The cells treated with SIM loaded nanoniosomes showed a significant elevation in the cell viability and a reduction in HMGB1, Nrf2, TNF-α, IL-1β, RIPK1, and ROCK1 expression levels compared to the OGD/R and SIM groups.Conclusion: Based on our findings, nanoniosomes could safely serve as a drug delivery system to counterbalance the disadvantages of SIM, resulting in improved aqueous solubility and stability.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":" ","pages":"15-22"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10994638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139691822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0