Iranian Journal of Biotechnology最新文献

{"title":"Anti-Inflammatory Properties of Extract of the Hairy Root of Native <i>Portulaca Oleracea</i> L. and Its Effect on Some Inflammatory Genes Expression in Rat Microglial Cells.","authors":"Somayeh Babashpour, Khosro Piri, Farzaneh Sabouni, Sevil Babashpour","doi":"10.30498/ijb.2023.323542.3249","DOIUrl":"https://doi.org/10.30498/ijb.2023.323542.3249","url":null,"abstract":"<p><strong>Background: </strong>Most herbs play significant roles in the treatment of various diseases. Because dopamine functions in the anti-inflammatory process and the presence of this substance in <i>Portulaca Oleracea L.</i> native plant, investigating this plant's anti-inflammatory properties in treating neurological diseases is interesting.</p><p><strong>Objectives: </strong>The objective of this study was to estimate the NO production and the expression level of inflammatory genes in lipopolysaccharide (LPS)-treated microglial cells affected by <i>P. oleracea L.</i> extraction.</p><p><strong>Materials and methods: </strong><i>P. oleracea L.</i> hairy root extract was isolated, and the primary microglial cell of the rat was isolated from glial cells and confirmed by immunocytochemistry analysis. Microglial cells were pretreated with different concentrations of <i>P. oleracea L.</i> extract and then treated with 1 μg.mL^-1 LPS. The control group did not receive any treatment. The NO level in culture supernatants was measured by the Griess method. The mRNA expression levels of <i>iNOS</i> (inducible Nitric oxide synthase) and <i>TNF-α</i> (tumor necrosis factor-alpha) in LPS-treated microglial cells were evaluated using Real-Time PCR.</p><p><strong>Results: </strong>The present study determined that 0.1 mg. mL^-1 of the <i>P. oleracea L.</i> extract decreased the NO production in rat microglial cells. Different concentrations of the <i>P. oleracea L.</i> extract had no prominent effects on LPS-treated cell viability. The results of real-time PCR indicated that <i>P. oleracea L</i> extracts suppressed the mRNA expression levels of <i>iNOS</i> and <i>TNF-α</i> in LPS-treated cells. MTT assay determined that <i>P. oleracea L.</i> extract was not cytotoxic, and the anti-inflammatory <i>P. oleracea L.</i> extract effects observed were not because of cell death.</p><p><strong>Conclusion: </strong><i>P. oleracea L.</i> extract might be helpful as an anti-inflammatory agent in treating inflammatory diseases.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning, Prokaryotic Expression and Functional Characterization of <i>NifH</i> Gene from the Associative Nitrogen-Fixing Bacteria <i>Klebsiella Variicola</i> DX120E.","authors":"Ying Qin, Yu-Yan Huang, Qaisar Khan, Kun-Kun Zhang, Dao-Jun Guo, Li-Tao Yang, Yang-Rui Li, Yong-Xiu Xing","doi":"10.30498/ijb.2023.352117.3451","DOIUrl":"https://doi.org/10.30498/ijb.2023.352117.3451","url":null,"abstract":"<p><strong>Background: </strong>Biological nitrogen fixation (BNF) is a unique mechanism in which microorganisms utilize the nitrogenase enzyme to catalyze the conversion of atmospheric nitrogen (N₂) to ammonia (NH₃). Fe protein, encoded by the <i>nifH</i> gene, is an essential component of the nitrogenase in <i>Klebsiella variicola</i> DX120E. However, the function of this gene in regulating nitrogen fixing activity is still unclear.</p><p><strong>Objectives: </strong>The objective of this study was to reveal the function of <i>nifH</i> gene in associative nitrogen-fixing bacteria <i>Klebsiella variicola</i> DX120E and micro-sugarcane system by immunoassay and gene editing.</p><p><strong>Materials and methods: </strong>In the current investigation, the <i>nifH</i> gene was cloned in a pET-30a (+) vector and expressed in <i>Escherichia coli</i>. The NifH protein was purified and used to immunize rabbit, and then the serum was collected and purified to obtain rabbit anti-NifH polyclonal antibodies. The CRISPR-Cas9 system was applied to produce <i>nifH</i> mutant strains, and the nitrogen-fixing enzyme activity, gene, and protein expression were analyzed.</p><p><strong>Results: </strong>Both <i>in vitro</i> and <i>in vivo</i> NifH proteins were detected by Western blotting, which were 43 and 32 kDa respectively. The expression of <i>nifD</i> and <i>nifK</i> genes was decreased, and nitrogenase activity was reduced in the <i>nifH</i> mutant strain.</p><p><strong>Conclusion: </strong>The <i>nifH</i> gene mutant weakened the nitrogenase activity by regulating the expression of Fe protein, which suggests a potential strategy to study the nitrogen fixation-related genes and the interactions between endophytic nitrogen-fixing bacteria and sugarcane.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PPI Identification of Immune-Related Biomarkers in Esophageal Cancer on the Basis of Gene Co-Expression Network.","authors":"Shengjia Chen, Hongfu Zeng, Aiping Zhang, Jinlan Lin, Kai Zhu","doi":"10.30498/ijb.2023.341601.3377","DOIUrl":"https://doi.org/10.30498/ijb.2023.341601.3377","url":null,"abstract":"<p><strong>Background: </strong>The mortality rate of esophageal cancer is on the continuous increase. Fortunately, with the development of immunotherapy, the prognosis and survival rate of patients with esophageal cancer have been improved gradually.</p><p><strong>Objective: </strong>Immune markers have a crucial part in immunotherapy. Therefore, it is of great meaning to delve further into immune-related biomarkers of esophageal cancer for better treatment.</p><p><strong>Materials and methods: </strong>In this study, gene co-expression networks were established using weighted gene co-expression network analysis, thus forming gene modules with different clusters. The tumor immune microenvironment was assessed with the ESTIMATE algorithm.</p><p><strong>Results: </strong>Analysis of the module Eigen gene -immune score trait indicated that the black module was markedly associated with immune score, with the top 80 genes regarding correlation ranking as the candidate hub gene set. Enrichment analysis revealed that genes within the black module were primarily enriched in tumor immune-related functions. To mine the hub genes that were closely connected with immunity, protein-protein interaction networks were constructed by STRING for genes within the black module, and genes with the interaction score top10 were retained. They were intersected with hub genes to finally obtain four hub genes: <i>CCR5, LCP2, PTPRC</i> and <i>TYROBP</i>. The samples were divided into high- and low-expression groups by the median expression of hub gene, and survival analysis was performed in combination with clinical information. The results revealed that the high-expression groups of genes <i>LCP2</i> and <i>PTPRC</i> had a poor prognosis. TIMER immune cell infiltration analysis revealed that the expression levels of the 4 hub genes were positively correlated with immune cell infiltration and negatively correlated with tumor purity. In addition, these 4 hub genes were correlated with the expression of immune checkpoint genes <i>CTLA-4</i> and <i>PDCD1</i> positively. Gene set enrichment analysis enrichment analysis demonstrated that there were differences in tumor immunity and cancer-related pathways between high and low expression of 4 hub genes.</p><p><strong>Conclusion: </strong>Altogether, we identified four biomarkers that may have connection with tumor immunity, and speculated that these genes may influence patient prognosis by affecting pathways related to esophageal cancer immunity. This study will pave the way for the research of immune mechanisms of esophageal cancer and the analysis of patient's prognosis.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>MiRNA-145-5p</i> Restrains Malignant Behaviors of Breast Cancer Cells Via Downregulating <i>H2AFX</i> Expression.","authors":"Deshun Yao, Xin Chen","doi":"10.30498/ijb.2023.349450.3433","DOIUrl":"https://doi.org/10.30498/ijb.2023.349450.3433","url":null,"abstract":"<p><p>Breast cancer is a prevalent tumor with high aggressiveness among female populations. <i>MiRNA-145-5p</i> plays an important role in multiple cancers.</p><p><strong>Materials and methods: </strong>qRT-PCR detected <i>miRNA-145-5p</i> and histone protein family member X (<i>H2AFX</i>) mRNA expression in breast cancer cells, and western blot determined the protein expression of H2AFX. After predicting the target genes via the bioinformatics methods, the targeting relationship between <i>miRNA-145-5p</i> and <i>H2AFX</i> was verified by dual-luciferase, RIP, and RNA pull-down assays. The relationship between <i>H2AFX</i> and clinical indexes was also analyzed. Furthermore, the effects of <i>miRNA-145-5p/H2AFX</i> regulatory axis on breast cancer cell progression were determined by colony formation, wound healing, CCK-8, and Transwell assays.</p><p><strong>Results: </strong>The results suggested that miRNA-145-5p was markedly lowly-expressed in breast cancer tissue and cells, while <i>H2AFX</i> was upregulated, which had a positive correlation with T stages of breast cancer. Besides, overexpressed <i>miRNA-145-5p</i> was found to remarkably suppress progression of breast cancer cells. As bioinformatic analysis predicted that <i>H2AFX</i> was the potential target of <i>miRNA-145-5p</i>, the dual-luciferase assay was conducted, which demonstrated that <i>miRNA-145-5p</i> negatively regulated the expression of H2AFX by targeting its 3'-UTR. The rescue experiment demonstrated that overexpression of miRNA-145-5p could offset the promotion effects of oe-H2AFX on malignant progression.</p><p><strong>Objective: </strong>Our study is aimed at exploring how <i>miRNA-145-5p</i> functions in breast cancer cells.</p><p><strong>Conclusion: </strong>Our findings confirmed that <i>miRNA-145-5p</i> hindered malignant progression of breast cancer by negatively regulating <i>H2AFX</i>. <i>MiRNA-145-5p/H2AFX</i> axis may be a novel therapeutic target for breast cancer.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dexmedetomidine Suppresses Glutamate-Stimulated Neuronal Injury Via MicroRNA-433/Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Pathway.","authors":"Lin Zhao, Junlan Huang, Zhouquan Wu, Guowei Fu","doi":"10.30498/ijb.2023.318052.3214","DOIUrl":"https://doi.org/10.30498/ijb.2023.318052.3214","url":null,"abstract":"<p><strong>Background: </strong>Cerebral ischemia has been a hotpot in the prevention and treatment of cerebral ischemia. Dexmedetomidine (Dex) is a new type of highly selective α2 adrenergic receptor agonist with pharmacological properties.</p><p><strong>Objective: </strong>Quantitative studies have shown that Dex has a protective effect on glutamate (Glu)-induced neuronal damage. however, its mechanism has not been fully elucidated. The purpose of this study was to explore the underlying molecular mechanism by which Dex ameliorates Glu-induced neuronal injury by regulating miR-433/JAK2/STAT3 axis.</p><p><strong>Materials and methods: </strong>A model of neuronal injury was constructed by Glu treatment and intervened with Dex. miRNA expression profiling assay was conducted to screen potential miRNAs affected by Dex. Cell viability, lactate dehydrogenase (LDH) release and apoptosis were detected by MTT assay, LDH kit, and TUNEL staining, respectively. Oxidative stress indicators were assessed by ELISA whereas mitochondrial membrane potential (MMP) was assessed by C11-BODIPY581/591 staining. The targeting relationship between the miR-433 and JAK2 was verified by dual-luciferase reporter assay and gene expression was analyzed by quantitative PCR and Western blot.</p><p><strong>Results: </strong>Glu treatment decreased cell viability and MMP and promoted LDH release, apoptosis and oxidative damage. Glu-induced changes in neurons were reversed after Dex treatment through upregulating the miR-433 expression to block the activation of JAK2/STAT3 pathway.</p><p><strong>Conclusions: </strong>Dex protects against Glu-induced neuronal injury by regulating miR-433/JAK2/STAT3 pathway, which provides new insights into the treatment of neuronal injury.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Collagen and Fibrin Hydrogels Encapsulated with Adipose Tissue Mesenchymal Stem Cell-Derived Exosomes for Treatment of Spinal Cord Injury in a Rat Model.","authors":"Zohreh Afsartala, Mahmoudreza Hadjighassem, Sadegh Shirian, Somayeh Ebrahimi-Barough, Leila Gholami, Gilda Parsamanesh, Ziba Veisimalekshahi, Latifeh Karimzadehbardeei, Jafar Ai","doi":"10.30498/ijb.2023.362229.3505","DOIUrl":"https://doi.org/10.30498/ijb.2023.362229.3505","url":null,"abstract":"<p><strong>Background: </strong>Mesenchymal stem cell (MSC) derived exosomes (MSC-DE) have been demonstrated to be potential candidates for the treatment of rat spinal cord injury (SCI).</p><p><strong>Objective: </strong>The effect of AD-MSC and AD-MSC-DE encapsulated into collagen and fibrin hydrogels on the treatment of SCI in a rat animal model was investigated for introducing a new effective SCI treatment method.</p><p><strong>Materials and methods: </strong>The AD-MSC-DE was isolated using ultra-centrifugation at 100,000×g for 120 min and characterized by different methods. Fibrin and collagen hydrogels were synthesized and then mixed with AD-MSC-DE suspension. the characterized AD-MSC-DE were encapsulated into collagen and fibrin hydrogels. eighteen adult male Wister rats were randomly classified into 3 equal groups (n=6): the control group (SCI rat without treatment), SCI rat treated with either AD-MSC-DE encapsulated in collagen hydrogel or encapsulated in fibrin hydrogel groups. the treatment approaches were evaluated using clinical, histological, and molecular assays.</p><p><strong>Results: </strong>The AD-MSC-DE encapsulated into fibrin and collagen groups showed better clinical function than the control group. The AD-MSC-DE encapsulated into fibrin and collagen also improved SCI-induced polio and leuko-myelomalacia and leads to higher expression of NF protein than the control group. In the AD-MSC-DE encapsulated into collagen and fibrin leads to up-regulation the mean levels of NEFL (23.82 and 24.33, respectively), eNOS (24.31 and 24.53, respectively), and CK19 mRNAs (24.23 and 23.98, respectively) compared to the control group.</p><p><strong>Conclusion: </strong>The AD-MSC-DE encapsulated within ECM-based hydrogel scaffolds such as collagen and fibrin can regenerate the injured nerve in SCI rats and reduce spinal cord lesion-induced central neuropathic pain.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Affinity Chromatography Based on Sepharose 4B- chitin for Rapid Purification of <i>Urtica dioica</i> Agglutinin.","authors":"Mohammadkazem Heydari, Abasalt Hosseinzadeh Colagar, Davood Sabour","doi":"10.30498/ijb.2023.339309.3364","DOIUrl":"https://doi.org/10.30498/ijb.2023.339309.3364","url":null,"abstract":"<p><strong>Background: </strong>Today, numerous antimicrobial and anticancer properties have been reported for plant lectins due to their ability to bind to carbohydrates. The <i>Urtica dioica</i> agglutinin (UDA lectin) is a monomeric, small, and low molecular weight glycoprotein. It has attracted the attention of many researchers for identification, treatment, and other clinical purposes.</p><p><strong>Objectives: </strong>The aim of this study is the optimization of the chitin affinity chromatography based on Sepharose 4B (CNBr-activated Sepharose 4B) for the rapid purification of UDA lectin from <i>Urtica dioica</i> rhizome.</p><p><strong>Materials and methods: </strong>The chitin ligands were dissolved in 40% Trichloroacetic acid and attached to Sepharose 4B according to the Amersham-Biosciences instructions. The attachment of the ligand to the Sepharose 4B beads was investigated by Fourier transform infrared (FTIR) spectroscopy. An acidic crude extract of nettle rhizome passes from chromatographic columns in two sizes with dimensions: 24 x 0.51 cm and 8.44 x 0.86 cm. Quantity and quality of purified lectin were calculated by the Bradford microplate method: SDS-PAGE gel electrophoresis and human erythrocyte cell (RBC) hemagglutination, respectively.</p><p><strong>Results: </strong>The analysis of FTIR spectrograms showed that major changes were observed in the fingerprint regions. Besides, due to the dissolution of Sepharose 4B and chitin in the aqueous phase, this difference was not significant in the Imine and Nitrile regions. On the other hand, the comparative results of purification chromatograms showed that increasing the column length causes a smaller half-width and increases the length of the purified peak. Also, it leads to high-quality purified UDA lectin, with a molecular weight of almost 12.5 kDa in gel electrophoresis. Hemagglutination activity on trypsinized red blood cells was displayed, and agglutination of purified UDA lectin started at least at 300 μg.mL^-1 concentration.</p><p><strong>Conclusion: </strong>According to our findings, we suggested that dissolving chitin in the polar solvent of Trichloroacetic acid, using Sepharose 4B as the beads of a matrix, and increasing the column length might lead to a decrease in the half-width of the peak. These can increase the purity and concentration of purified UDA lectin, and speed up the purification process. These findings could be used by researchers to accelerate the purification of UDA lectin in other studies, dealing with drug delivery systems, ELISA techniques, and cell growth.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data-Mining of Barley to Identify Salt Stress Hub Genes, Gene Expression Analysis and Recombinant Plasmid Construction.","authors":"Ehsan Sohrabi, Masoud Tohidfa, Asadolah Ahmadikhah, Rahele Ghanbari Moheb Seraj","doi":"10.30498/ijb.2023.343700.3389","DOIUrl":"https://doi.org/10.30498/ijb.2023.343700.3389","url":null,"abstract":"<p><strong>Background: </strong>Salinity is one of the major abiotic stresses that limit the production and yields of agricultural crops worldwide.</p><p><strong>Objectives: </strong>In order to identify key barley genes under salinity stress, the available metadata were examined by two methods of Cytoscape and R software. Next, the hub expression of the selected gene was evaluated under different salinity stress treatments and finally, this gene was cloned into cloning and expression vector and recombinant plasmid was made.</p><p><strong>Materials and methods: </strong>In this study, we extracted salinity stress tolerant genes from several kinds of literature and also microarray data related to barley under salinity conditions from various datasets. The list of genes related to literature analyzed using string and Cytoscape. The genes from the datasets were first filtered and then the hub genes were identified by Cytoscape and R methods. Next, these hub genes were analyzed for the promoter.</p><p><strong>Results: </strong>Ten hub genes were selected and their promoters were analyzed, the <i>cis</i>-element of which was often cis-acting regulatory element involved in the methyl jasmonate -responsiveness, common <i>cis</i>-acting element in promoter and enhancer regions and MYBHv1 binding site. Finally, the sedoheptulose-1,7-bisphosp gene (<i>SBPase</i>), which had the highest interaction in both gene lists and both types of gene networks, was selected as hub gene. Next, the expression of <i>SBPase</i> gene was examined in two variety of Youssef variety (salt tolerant) and Fajr variety (salt sensitive) under salinity stress (NaCl 100mM) at 0 (control), 3, 6, 12 and 24 hours after stress. The results showed that the expression of this gene increased with increasing the duration of stress in both varieties. Comparison of the two varieties showed that the expression of <i>SBPase</i> gene in the tolerant genotype was twice as high as sensitive. Finally, <i>SBPase</i> gene as a key gene for salinity stress was cloned in both cloning (pTG19) and expression (pBI121) vectors.</p><p><strong>Conclusions: </strong>According to our results, SBPase gene increased growth and photosynthesis in barley under various abiotic stresses, therefore, over-expression of this gene in barley is recommended to produce plants resistant to abiotic stresses.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SOX2 Overlapping Transcript (<i>SOX2-OT</i>) Enhances the Lung Cancer Malignancy Through Interaction with <i>miR-194-5p/</i>SOX5 Axis.","authors":"Zahra Sadeghi, Fatemeh Dodangeh, Jamshid Raheb","doi":"10.30498/ijb.2023.364919.3530","DOIUrl":"https://doi.org/10.30498/ijb.2023.364919.3530","url":null,"abstract":"<p><strong>Background: </strong>Lung cancer is one of the most common types of cancer and a leading cause of cancer-related deaths worldwide. Therefore, it is useful to know the biomarkers involved in the malignancy of lung cancer.</p><p><strong>Objectives: </strong>This study aimed to show that <i>SOX2-OT</i> as a long non-coding RNA (IncRNA) regulates gene expression via the <i>SOX2-OT/miR-194-5p/</i>SOX5 axis molecular pathway in lung cancer.</p><p><strong>Materials and methods: </strong>A549 cells transfected with siRNA-<i>SOX2-OT</i> and the expression of <i>SOX2-OT</i> and <i>miR-194-5p</i> genes were analyzed by real-time PCR before and after transfection. In addition, the expression of the B-catenin, MMP9, phosphorylated and activated STAT3 (p-STAT3), SOX5, and VEGF proteins before and after transfection was investigated by Western blotting.</p><p><strong>Results: </strong>After using siRNA-<i>SOX2-OT</i>, an increase in the expression of <i>miR-194-5p</i> and a decrease in the expression of B-catenin, SOX5, p-STAT3 activated STAT3, VEGF, and MMP9 proteins was observed.</p><p><strong>Conclusions: </strong>According to the results of the present study, an increase in <i>SOX2-OT</i> in lung cancer seems to stimulate the expression of beta-catenin, SOX5, MMP9, and VEGF thus support the malignancy of lung cancer cells.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of an <i>STK11</i> Mutation and Immune-Related Prognostic Prediction Model in Lung Adenocarcinoma.","authors":"Bo Tang,&nbsp;Xia Zhao,&nbsp;Hongbing Liu,&nbsp;Qingfeng Zhang,&nbsp;Kui Liu,&nbsp;Xiaoyan Yang,&nbsp;Yun Huang","doi":"10.30498/ijb.2022.307202.3168","DOIUrl":"https://doi.org/10.30498/ijb.2022.307202.3168","url":null,"abstract":"<p><strong>Background: </strong><i>STK11</i> mutation in LUAD affects immune cell infiltration in tumor tissue, and is associated with tumor prognosis.</p><p><strong>Objective: </strong>This study aimed to construct a <i>STK11</i> mutation and immune-related LUAD prognostic model.</p><p><strong>Materials and methods: </strong>The mutation frequency of <i>STK11</i> in LUAD was queried via cBioPortal in TCGA and PanCancer Atlas databases. The degree of immune infiltration was analyzed by CIBERSORT analysis. DEGs in <i>STK11</i>mut and <i>STK11</i>wt samples were analyzed. Metascape, GO and KEGG methods were adopted for functional and signaling pathway enrichment analysis of DEGs. Genes related to immune were overlapped with DEGs to acquire immune-related DEGs, whose Cox regression and LASSO analyses were employed to construct prognostic model. Univariate and multivariate Cox regression analyses verified the independence of riskscore and clinical features. A nomogram was established to predict the OS of patients. Additionally, TIMER was introduced to analyze relationship between infiltration abundance of 6 immune cells and expression of feature genes in LUAD.</p><p><strong>Results: </strong>The mutation frequency of <i>STK11</i> in LUAD was 16%, and the degrees of immune cell infiltration were different between the wild-type and mutant <i>STK11</i>. DEGs of <i>STK11</i> mutated and unmutated LUAD samples were mainly enriched in immune-related biological functions and signaling pathways. Finally, 6 feature genes were obtained, and a prognostic model was established. Riskscore was an independent immuno-related prognostic factor for LUAD. The nomogram diagram was reliable.</p><p><strong>Conclusion: </strong>Collectively, genes related to <i>STK11</i> mutation and immunity were mined from the public database, and a 6-gene prognostic prediction signature was generated.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/23/0b/IJB-21-e3168.PMC10203181.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9578693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}