Invertebrate Neuroscience最新文献_第8页

NemaCount: quantification of nematode chemotaxis behavior in a browser. NemaCount:在浏览器中量化线虫趋化行为。

Invertebrate Neuroscience Pub Date : 2016-06-01 Epub Date: 2016-05-21 DOI: 10.1007/s10158-016-0188-1

Damien M O'Halloran

{"title":"NemaCount: quantification of nematode chemotaxis behavior in a browser.","authors":"Damien M O'Halloran","doi":"10.1007/s10158-016-0188-1","DOIUrl":"https://doi.org/10.1007/s10158-016-0188-1","url":null,"abstract":"Nematodes such as Caenorhabditis elegans offer a very effective and tractable system to probe the underlying mechanisms of diverse sensory behaviors. Numerous platforms exist for quantifying nematode behavior and often require separate dependencies or software. Here I describe a novel and simple tool called NemaCount that provides a versatile solution for the quantification of nematode chemotaxis behavior. The ease of installation and user-friendly interface makes NemaCount a practical tool for measuring diverse behaviors and image features of nematodes such as C. elegans. The main advantage of NemaCount is that it operates from within a modern browser such as Google Chrome or Apple Safari. Any features that change in total number, size, shape, or angular distance between control and experimental preparations are suited to NemaCount for image analysis, while commonly used chemotaxis assays can be quantified, and statistically analyzed using a suite of functions from within NemaCount. NemaCount also offers image filtering options that allow the user to improve object detection and measurements. NemaCount was validated by examining nematode chemotaxis behavior; angular distances of locomotory tracks in C. elegans; and body lengths of Heterorhabditis bacteriophora nematodes. Apart from a modern browser, no additional software is required to operate NemaCount, making NemaCount a cheap, simple option for the analysis of nematode images and chemotaxis behavior. ","PeriodicalId":14430,"journal":{"name":"Invertebrate Neuroscience","volume":"16 2","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10158-016-0188-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34569687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Report on the 13th symposium on invertebrate neurobiology held 26-30 August 2015 at the Balaton Limnological Institute, MTA Centre for ecological research of the Hungarian Academy of Sciences, Tihany, Hungary. 2015年8月26日至30日在匈牙利科学院生态研究中心巴拉顿湖沼研究所举行的第13届无脊椎神经生物学研讨会报告。

Invertebrate Neuroscience Pub Date : 2016-06-01 DOI: 10.1007/s10158-016-0186-3

Anna Crisford, Lindy Holden-Dye, Robert J Walker

引用次数: 0

Context-dependent regulation of feeding behaviour by the insulin receptor, DAF-2, in Caenorhabditis elegans. 草履虫胰岛素受体 DAF-2 对摄食行为的情境依赖性调控

Invertebrate Neuroscience Pub Date : 2016-06-01 Epub Date: 2016-05-21 DOI: 10.1007/s10158-016-0187-2

James Dillon, Lindy Holden-Dye, Vincent O'Connor, Neil A Hopper

{"title":"Context-dependent regulation of feeding behaviour by the insulin receptor, DAF-2, in Caenorhabditis elegans.","authors":"James Dillon, Lindy Holden-Dye, Vincent O'Connor, Neil A Hopper","doi":"10.1007/s10158-016-0187-2","DOIUrl":"10.1007/s10158-016-0187-2","url":null,"abstract":"Insulin signalling plays a significant role in both developmental programmes and pathways modulating the neuronal signalling that controls adult behaviour. Here, we have investigated insulin signalling in food-associated behaviour in adult C. elegans by scoring locomotion and feeding on and off bacteria, the worm's food. This analysis used mutants (daf-2, daf-18) of the insulin signalling pathway, and we provide evidence for an acute role for insulin signalling in the adult nervous system distinct from its impact on developmental programmes. Insulin receptor daf-2 mutants move slower than wild type both on and off food and showed impaired locomotory responses to food deprivation. This latter behaviour is manifest as a failure to instigate dispersal following prolonged food deprivation and suggests a role for insulin signalling in this adaptive response. Insulin receptor daf-2 mutants are also deficient in pharyngeal pumping on food and off food. Pharmacological analysis showed the pharynx of daf-2 is selectively compromised in its response to 5-HT compared to the excitatory neuropeptide FLP-17. By comparing the adaptive pharyngeal behaviour in intact worms and isolated pharyngeal preparations, we determined that an insulin-dependent signal extrinsic to the pharyngeal system is involved in feeding adaptation. Hence, we suggest that reactive insulin signalling modulates both locomotory foraging and pharyngeal pumping as the animal adapts to the absence of food. We discuss this in the context of insulin signalling directing a shift in the sensitivity of neurotransmitter systems to regulate the worm's response to changes in food availability in the environment. ","PeriodicalId":14430,"journal":{"name":"Invertebrate Neuroscience","volume":"16 2","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4875951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34569686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and molecular characterization of a putative voltage-gated sodium channel gene in the crayfish 小龙虾电压门控钠通道基因的克隆与分子特性研究

Invertebrate Neuroscience Pub Date : 2016-03-31 DOI: 10.1007/s10158-016-0185-4

C. Coskun, N. Puralı

引用次数: 4

The impact of Directive 2010/63/EU on cephalopod research 指令2010/63/EU对头足类动物研究的影响

Invertebrate Neuroscience Pub Date : 2015-12-08 DOI: 10.1007/s10158-015-0183-y

G. Cristina, P. Andrews, G. Ponte, V. Galligioni, G. Fiorito

引用次数: 16

Single-cell analysis of peptide expression and electrophysiology of right parietal neurons involved in male copulation behavior of a simultaneous hermaphrodite 雌雄同体雄性交配行为中右顶叶神经元肽表达和电生理的单细胞分析

Invertebrate Neuroscience Pub Date : 2015-12-06 DOI: 10.1007/s10158-015-0184-x

Z. Filali, P. D. de Boer, bullet A W Pieneman, bullet R P J De Lange, bullet R F Jansen, bullet A Ter Maat, bullet R C Van Der Schors, bullet K W Li, bullet N M Van Straalen, J. Koene

引用次数: 4

Invertebrate neuroscience and CephsInAction at the Mediterranean Society for Neuroscience Meeting Cagliari 2015. 2015年地中海神经科学学会会议上的无脊椎神经科学和CephsInAction。

Invertebrate Neuroscience Pub Date : 2015-12-01 DOI: 10.1007/s10158-015-0182-z

Lindy Holden-Dye, Graziano Fiorito, Giovanna Ponte

引用次数: 0

Decrease in levels of the evolutionarily conserved microRNA miR-124 affects oligodendrocyte numbers in Zebrafish, Danio rerio. 进化上保守的microRNA miR-124水平的降低影响斑马鱼少突胶质细胞数量。

Invertebrate Neuroscience Pub Date : 2015-09-01 Epub Date: 2015-07-10 DOI: 10.1007/s10158-015-0180-1

Jacqueline K Morris, Anthony Chomyk, Ping Song, Nate Parker, Sadie Deckard, Bruce D Trapp, Sanjay W Pimplikar, Ranjan Dutta

{"title":"Decrease in levels of the evolutionarily conserved microRNA miR-124 affects oligodendrocyte numbers in Zebrafish, Danio rerio.","authors":"Jacqueline K Morris, Anthony Chomyk, Ping Song, Nate Parker, Sadie Deckard, Bruce D Trapp, Sanjay W Pimplikar, Ranjan Dutta","doi":"10.1007/s10158-015-0180-1","DOIUrl":"https://doi.org/10.1007/s10158-015-0180-1","url":null,"abstract":"Oligodendrocytes produce multi-lamellar myelin membranes that surround axons in the central nervous system (CNS). Preservation and generation of myelin are potential therapeutic targets for dysmyelinating and demyelinating diseases. MicroRNAs (miRNAs) play a vital role in oligodendrocyte differentiation and overall CNS development. miR-124 is a well-conserved neuronal miRNA with important roles in neuronal differentiation and function. miR-124 levels increase following loss of myelin in both human and rodent brains. While the role of neuronal miR-124 in neurogenesis has been established, its effects on axonal outgrowth and oligodendrocytes are not currently known. We therefore explored the possible effect of selective knockdown of miR-124 in Danio rerio using a morpholino-based knockdown approach. No morphological abnormalities or loss of motor neurons were detected despite loss of axonal outgrowth. Morpholino-based knockdown of miR-124 led to reciprocal increases in mRNA levels of target genes that inhibit axonal and dendritic projections. Importantly, loss of miR-124 led to decreased oligodendrocyte cell numbers and myelination of axonal projections in the ventral hindbrain. Taken together, our results add a new dimension to the existing complexity of neuron-glial relationships and highlight the utility of Danio rerio as a model system to investigate such interactions. ","PeriodicalId":14430,"journal":{"name":"Invertebrate Neuroscience","volume":"15 3","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10158-015-0180-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33993162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Transient effects of levamisole on Brugia malayi microfilariae. 左旋咪唑对马来布鲁氏微丝虫的瞬时效应。

Invertebrate Neuroscience Pub Date : 2015-09-01 Epub Date: 2015-07-18 DOI: 10.1007/s10158-015-0181-0

Eman Mostafa, Bob Storey, Adel Mohammed Farghaly, Hany Abd El-Hai Afify, Afaf Abd El-Raouf Taha, Adrian J Wolstenholme

引用次数: 5

Habituation of LG-mediated tailflip in the crayfish. 小龙虾脑电介导的尾巴翻转的习惯化。

Invertebrate Neuroscience Pub Date : 2015-06-01 Epub Date: 2015-03-22 DOI: 10.1007/s10158-015-0178-8

Toshiki Nagayama, Makoto Araki

{"title":"Habituation of LG-mediated tailflip in the crayfish.","authors":"Toshiki Nagayama, Makoto Araki","doi":"10.1007/s10158-015-0178-8","DOIUrl":"https://doi.org/10.1007/s10158-015-0178-8","url":null,"abstract":"Crayfish escape from threatening stimuli by tailflipping. If a stimulus is applied to the rear, crayfish escape up and forwards in a summersault maneuver that is mediated by the activation of lateral giant (LG) interneurons. The occurrence probability of LG-mediated tailflip, however, diminishes and habituates if a stimulus is repeatedly applied. Since crayfish have a relatively simple CNS with many identifiable neurons, crayfish represent a good animal to analyze the cellular basis of habituation. A reduction in the amplitude of the EPSP in the LGs, caused by direct chemical synaptic connection from sensory afferents by repetitive stimulations, is essential to bring about an inactivation of the LGs. The spike response of the LGs recovers within several minutes of habituation, but the LGs subsequently fail to spike when an additional stimulus is applied after specific periods following habituation. These results indicate that a decline in synaptic efficacy from the mechanosensory afferents recovers readily after a short delay, but then the excitability of the LGs themselves decreases. Furthermore, the processes underlying habituation are modulated depending on a social status. When two crayfish encounter each other, a winner-loser relationship is established. With a short interstimulus interval of 5 s, the rate of habituation of the LG in both socially dominant and subordinate crayfish becomes lower than in socially isolated animals. Serotonin and octopamine affect this social status-dependent modulation of habituation by means of activation of downstream second messenger system of cAMP and IP3 cascades, respectively. ","PeriodicalId":14430,"journal":{"name":"Invertebrate Neuroscience","volume":"15 2","pages":"178"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10158-015-0178-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33151499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9