International review of cell and molecular biology最新文献

DOGMA-seq and multimodal, single-cell analysis in acute myeloid leukemia. DOGMA-seq和急性髓系白血病的多模态单细胞分析。

3区生物学

International review of cell and molecular biology Pub Date : 2025-01-01 Epub Date: 2024-09-07 DOI: 10.1016/bs.ircmb.2024.08.001

JangKeun Kim, Nathan Schanzer, Ruth Subhash Singh, Mohammed I Zaman, J Sebastian Garcia-Medina, Jacqueline Proszynski, Saravanan Ganesan, Dan Landau, Christopher Y Park, Ari M Melnick, Christopher E Mason

{"title":"DOGMA-seq and multimodal, single-cell analysis in acute myeloid leukemia.","authors":"JangKeun Kim, Nathan Schanzer, Ruth Subhash Singh, Mohammed I Zaman, J Sebastian Garcia-Medina, Jacqueline Proszynski, Saravanan Ganesan, Dan Landau, Christopher Y Park, Ari M Melnick, Christopher E Mason","doi":"10.1016/bs.ircmb.2024.08.001","DOIUrl":"10.1016/bs.ircmb.2024.08.001","url":null,"abstract":"Acute myeloid leukemia (AML) is a complex cancer, yet advances in recent years from integrated genomics methods have helped improve diagnosis, treatment, and means of patient stratification. A recent example of a powerful, multimodal method is DOGMA-seq, which can measure chromatin accessibility, gene expression, and cell-surface protein levels from the same individual cell simultaneously. Previous bimodal single-cell techniques, such as CITE-seq (Cellular indexing of transcriptomes and epitopes), have only permitted the transcriptome and cell-surface protein expression measurement. DOGMA-seq, however, builds on this foundation and has implications for examining epigenomic, transcriptomic, and proteomic interactions between various cell types. This technique has the potential to be particularly useful in the study of cancers such as AML. This is because the cellular mechanisms that drive AML are rather heterogeneous and require a more complete understanding of the interplay between the genetic mutations, disruptions in RNA transcription and translation, and surface protein expression that cause these cancers to develop and evolve. This technique will hopefully contribute to a more clear and complete understanding of the growth and progression of complex cancers.","PeriodicalId":14422,"journal":{"name":"International review of cell and molecular biology","volume":"390 ","pages":"67-108"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetics and CTCs: New biomarkers and impact on tumor biology. 表观遗传学和CTCs：新的生物标志物及其对肿瘤生物学的影响。

3区生物学

International review of cell and molecular biology Pub Date : 2025-01-01 Epub Date: 2024-06-01 DOI: 10.1016/bs.ircmb.2024.03.002

Aliki Ntzifa, Evi Lianidou

{"title":"Epigenetics and CTCs: New biomarkers and impact on tumor biology.","authors":"Aliki Ntzifa, Evi Lianidou","doi":"10.1016/bs.ircmb.2024.03.002","DOIUrl":"https://doi.org/10.1016/bs.ircmb.2024.03.002","url":null,"abstract":"DNA methylation is one of the best-known epigenetic markers and plays a critical role in the control of gene activity and the architecture of the nucleus of the cell. Epigenetic alterations and especially DNA methylation have a high potential to provide valuable and innovative cancer biomarkers. Liquid biopsy is a unique minimally invasive tool for the management of cancer patients based on the extraction of information through detailed molecular analysis of circulating genetic material in peripheral blood and allows us to characterize the evolution of a solid tumor in real time. DNA methylation in combination with liquid biopsy is very powerful when it comes to providing circulating epigenetic biomarkers of clinical importance. Numerous DNA methylation markers are now being tested in liquid biopsies as potential biomarkers in various types of cancer. DNA methylation is mostly being studied in ctDNA but there are also a small number of studies up to now performed in gDNA isolated from CTCs. A highly important dimension of the combination of liquid biopsy/DNA methylation analysis is its high potential for early cancer detection since alterations in DNA methylation in plasma can be detected very early during cancer pathogenesis. Methylated DNA, modified nucleosomes and noncoding RNAs can be used as blood circulating epigenetic biomarkers for real-time and minimally-invasive cancer monitoring. DNA methylation is very promising and powerful in providing novel biomarkers for improving cancer diagnostics, while another important dimension in this field is the use of DNA methylation inhibitors in cancer treatment. In this chapter we present the current findings on epigenetic alterations detected in CTCs in various types of cancer, and further discuss their potential as novel liquid biopsy-based DNA methylation biomarkers.","PeriodicalId":14422,"journal":{"name":"International review of cell and molecular biology","volume":"392 ","pages":"177-198"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circulating tumor cells in lung cancer: Integrating stemness and heterogeneity to improve clinical utility. 肺癌循环肿瘤细胞：整合干性和异质性以提高临床应用。

3区生物学

International review of cell and molecular biology Pub Date : 2025-01-01 Epub Date: 2024-03-19 DOI: 10.1016/bs.ircmb.2024.03.001

Teresa Beninato, Giuseppe Lo Russo, Rita Leporati, Luca Roz, Giulia Bertolini

{"title":"Circulating tumor cells in lung cancer: Integrating stemness and heterogeneity to improve clinical utility.","authors":"Teresa Beninato, Giuseppe Lo Russo, Rita Leporati, Luca Roz, Giulia Bertolini","doi":"10.1016/bs.ircmb.2024.03.001","DOIUrl":"10.1016/bs.ircmb.2024.03.001","url":null,"abstract":"Circulating tumor cells (CTC), released by primary tumors into the bloodstream, represent a valuable source to inform on cancer heterogeneity, cancer progression, metastatic disease and therapy efficacy without the need of invasive tumor biopsies. However, the extreme rarity and heterogeneity of CTCs, occurring at genotypic, phenotypic and functional levels, poses a major challenge for the study of this population and explains the lack of standardized strategies of CTC isolation. Lung cancer, the leading causes of cancer-related death worldwide, is a paradigmatic example of how CTC heterogeneity can undermine the clinical utility of this biomarker, since contrasting data have been reported using different isolation technologies. Some evidences suggest that only a fraction of CTC, characterized by stem-like feature and partial epithelial-mesenchymal transition (EMT) phenotype, can sustain metastasis initiation. Cancer stem cells (CSCs) have the potential to maintain primary tumors, initiate metastasis and escape both chemotherapy and immunotherapy treatments. Moreover, a close connection has been reported in several tumor types among hybrid phenotype, characterized by retention of epithelial and mesenchymal traits, acquisition of CSC feature and increased metastatic potential. This review focuses on the phenotypic and functional heterogeneity of CTCs and the resulting implications for their isolation and clinical validation, especially in the setting of non-small cell lung cancer (NSCLC). In particular, we discuss the most relevant studies providing evidence for the presence and prognostic/predictive value of CTC subsets characterized by stem-like and hybrid EMT phenotype. Despite technical and conceptual issues, tracking circulating CSCs has the potential to improve the prognostic/predictive value of CTCs in NSCLC setting and could provide novel insights into the comprehension of the metastatic process and identification of novel therapeutic targets.","PeriodicalId":14422,"journal":{"name":"International review of cell and molecular biology","volume":"392 ","pages":"1-66"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circuits involving the hypothalamic suprachiasmatic nucleus for controlling diverse physiologies verified by the aid of optogenetics and chemogenetics. 涉及下丘脑视交叉上核的控制多种生理的电路已被光遗传学和化学遗传学证实。

3区生物学

International review of cell and molecular biology Pub Date : 2025-01-01 Epub Date: 2024-06-26 DOI: 10.1016/bs.ircmb.2024.06.002

Sui-Wen Hsiao, Masao Doi

{"title":"Circuits involving the hypothalamic suprachiasmatic nucleus for controlling diverse physiologies verified by the aid of optogenetics and chemogenetics.","authors":"Sui-Wen Hsiao, Masao Doi","doi":"10.1016/bs.ircmb.2024.06.002","DOIUrl":"10.1016/bs.ircmb.2024.06.002","url":null,"abstract":"The suprachiasmatic nucleus (SCN) functions as the master circadian pacemaker in mammals. Since 2015, facilitated by cutting-edge optogenetic and chemogenetic techniques, significant progress has been made in understanding the circuits involving the SCN that mediate diverse physiological functions. The time-specific and cell type-selective manipulation of neuronal activity within and outside the SCN drove the verification of both expected and previously unrecognized circuits operating for controlling various functions, including circadian locomotor activity, itch behavior, anticipatory thirst, aggression, corticosterone release, food-anticipatory activity, wakefulness, and photoperiod-related adaptive behavior. In addition, optogenetic/chemogenetic approaches verified the functional connection of the SCN to the control of body temperature, heart rate, and insulin sensitivity through as-yet-unknown circuit details. This review intends to provide an overview of SCN input/output pathways elucidated by optogenetics and chemogenetics. A fundamental question remains regarding the coherence of the identified numerous output pathways that are dictated by the SCN. Deciphering the potential coordination among the SCN's circuits via optogenetics and chemogenetics is needed to understand the mechanism underlying the harmonious regulation of multiple circadian physiologies.","PeriodicalId":14422,"journal":{"name":"International review of cell and molecular biology","volume":"393 ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preface. 前言。

3区生物学

International review of cell and molecular biology Pub Date : 2025-01-01 DOI: 10.1016/S1937-6448(25)00066-8

引用次数: 0

Circulating biomarkers for diagnosis, prognosis, and treatment response prediction in cancer. 用于癌症诊断、预后和治疗反应预测的循环生物标志物。

3区生物学

International review of cell and molecular biology Pub Date : 2025-01-01 DOI: 10.1016/S1937-6448(25)00031-0

Lorenzo Galluzzi, Sheila Spada

引用次数: 0

Advances in metastatic prostate cancer circulating tumor cell enrichment technologies and clinical studies. 转移性前列腺癌循环肿瘤细胞富集技术及临床研究进展。

3区生物学

International review of cell and molecular biology Pub Date : 2025-01-01 Epub Date: 2024-07-29 DOI: 10.1016/bs.ircmb.2024.06.005

Naira Essam Abou-Ghali, Paraskevi Giannakakou

{"title":"Advances in metastatic prostate cancer circulating tumor cell enrichment technologies and clinical studies.","authors":"Naira Essam Abou-Ghali, Paraskevi Giannakakou","doi":"10.1016/bs.ircmb.2024.06.005","DOIUrl":"https://doi.org/10.1016/bs.ircmb.2024.06.005","url":null,"abstract":"Circulating tumor cells (CTCs) have emerged as a pivotal tool that enables molecular interrogation of patient tumor cells and association with clinical outcomes. In prostate cancer specifically, where tumor biopsies from patients with bone metastasis are extremely challenging, CTCs offer a viable and established source of tumor \"biopsy\". While the prognostic value of CTC enumeration in metastatic prostate cancer is established, there is a compelling need for molecular CTC characterization for effective patient stratification and disease management. The clinical utility of CTCs has been advanced by the evolution of enrichment technologies and their molecular characterization. Enrichment technologies have evolved from strictly EpCAM-based enrichment to antigen-agnostic enrichment, while their clinical utility has evolved from enumeration to advanced downstream analyses including CTC proteomics, transcriptomics and genomics. This chapter offers a comprehensive overview of recent advancements in CTC enrichment and analytical technologies while highlighting pivotal clinical studies in prostate cancer, that utilize CTCs to determine the molecular basis of clinical response and resistance, to assist in disease management and treatment customization.","PeriodicalId":14422,"journal":{"name":"International review of cell and molecular biology","volume":"392 ","pages":"151-175"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic modulation of doxorubicin resistance and strategies for enhancing chemotherapeutic sensitivity. 阿霉素耐药的表观遗传调控及提高化疗敏感性的策略。

3区生物学

International review of cell and molecular biology Pub Date : 2025-01-01 Epub Date: 2024-10-07 DOI: 10.1016/bs.ircmb.2024.09.004

Sruthi Sritharan, Nageswaran Sivalingam

引用次数: 0

Epigenetic alteration in cervical cancer induced by human papillomavirus. 人乳头瘤病毒诱发宫颈癌的表观遗传改变。

3区生物学

International review of cell and molecular biology Pub Date : 2025-01-01 Epub Date: 2024-10-04 DOI: 10.1016/bs.ircmb.2024.09.001

Elisa-Elvira Figueroa-Angulo, Jonathan Puente-Rivera, Yussel Fernando Perez-Navarro, Edgar Mendieta Condado, María-Elizbeth Álvarez-Sánchez

引用次数: 0

Revisiting epigenetic regulation in cancer: Evolving trends and translational implications. 回顾表观遗传调控在癌症：演变趋势和翻译意义。

3区生物学

International review of cell and molecular biology Pub Date : 2025-01-01 Epub Date: 2024-10-04 DOI: 10.1016/bs.ircmb.2024.09.002

Mohammad Afsar Khan, Dhruv Mishra, Ranjan Kumar, Hifzur R Siddique

{"title":"Revisiting epigenetic regulation in cancer: Evolving trends and translational implications.","authors":"Mohammad Afsar Khan, Dhruv Mishra, Ranjan Kumar, Hifzur R Siddique","doi":"10.1016/bs.ircmb.2024.09.002","DOIUrl":"10.1016/bs.ircmb.2024.09.002","url":null,"abstract":"Cancer is a leading cause of mortality worldwide. The evolving role of epigenetics and tumor microenvironments of cancer pose significant challenges to the management of cancer. Besides genetics, epigenetic changes play a crucial role in the alteration of cellular machinery, progression, metastasis, epithelial-mesenchymal transition, and chemoresistance. Epigenetic changes such as DNA and RNA methylation, histone modifications, and chromatin modeling directly or indirectly influence the different stages of cancer from initiation to chemoresistant phenotype. In addition, alterations in the epigenetic machinery, such as hypo- or hyperactivation of proteins involved in epigenetic modifications, can lead to different health complications, including cancer. Recently, epi-drugs or epigenetic drugs offer emerging hope for the treatment and management of this deadly disease. Various epigenetic drugs targeting factors responsible for epigenetic modifications in cancer are currently under clinical trials. This chapter provides an overview of epigenetic modifications, their clinical implications, and the potential of epigenetic drugs for cancer treatment.","PeriodicalId":14422,"journal":{"name":"International review of cell and molecular biology","volume":"390 ","pages":"1-24"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0