International Journal of Peptides最新文献_第4页

Total chemical synthesis of a heterodimeric interchain bis-lactam-linked Peptide: application to an analogue of human insulin-like Peptide 3. 异二聚体间链双内酰胺连接肽的全化学合成:在人胰岛素样肽3类似物中的应用。

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-10-28 DOI: 10.1155/2013/504260

John Karas, Fazel Shabanpoor, Mohammed Akhter Hossain, James Gardiner, Frances Separovic, John D Wade, Denis B Scanlon

{"title":"Total chemical synthesis of a heterodimeric interchain bis-lactam-linked Peptide: application to an analogue of human insulin-like Peptide 3.","authors":"John Karas, Fazel Shabanpoor, Mohammed Akhter Hossain, James Gardiner, Frances Separovic, John D Wade, Denis B Scanlon","doi":"10.1155/2013/504260","DOIUrl":"https://doi.org/10.1155/2013/504260","url":null,"abstract":"Nonreducible cystine isosteres represent important peptide design elements in that they can maintain a near-native tertiary conformation of the peptide while simultaneously extending the in vitro and in vivo half-life of the biomolecule. Examples of these cystine mimics include dicarba, diselenide, thioether, triazole, and lactam bridges. Each has unique physicochemical properties that impact upon the resulting peptide conformation. Each also requires specific conditions for its formation via chemical peptide synthesis protocols. While the preparation of peptides containing two lactam bonds within a peptide is technically possible and reported by others, to date there has been no report of the chemical synthesis of a heterodimeric peptide linked by two lactam bonds. To examine the feasibility of such an assembly, judicious use of a complementary combination of amine and acid protecting groups together with nonfragment-based, total stepwise solid phase peptide synthesis led to the successful preparation of an analogue of the model peptide, insulin-like peptide 3 (INSL3), in which both of the interchain disulfide bonds were replaced with a lactam bond. An analogue containing a single disulfide-substituted interchain lactam bond was also prepared. Both INSL3 analogues retained significant cognate RXFP2 receptor binding affinity. ","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2013 ","pages":"504260"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/504260","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31913852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Erratum to “Characterization of Selective Antibacterial Peptides by Polarity Index” 用极性指数表征选择性抗菌肽的勘误

International Journal of Peptides Pub Date : 2012-12-24 DOI: 10.1155/2012/613053

Carlos Polanco, J. L. Samaniego, T. Buhse, F. G. Mosqueira, A. Negrón-Mendoza, S. Ramos-Bernal, J. Castañón-González

引用次数: 37

Amyloid Beta Peptide slows down sensory-induced hippocampal oscillations. 淀粉样β肽会减缓感觉诱导的海马振荡

International Journal of Peptides Pub Date : 2012-01-01 Epub Date: 2012-04-29 DOI: 10.1155/2012/236289

Fernando Peña-Ortega, Ramón Bernal-Pedraza

{"title":"Amyloid Beta Peptide slows down sensory-induced hippocampal oscillations.","authors":"Fernando Peña-Ortega, Ramón Bernal-Pedraza","doi":"10.1155/2012/236289","DOIUrl":"10.1155/2012/236289","url":null,"abstract":"Alzheimer's disease (AD) progresses with a deterioration of hippocampal function that is likely induced by amyloid beta (Aβ) oligomers. Hippocampal function is strongly dependent on theta rhythm, and disruptions in this rhythm have been related to the reduction of cognitive performance in AD. Accordingly, both AD patients and AD-transgenic mice show an increase in theta rhythm at rest but a reduction in cognitive-induced theta rhythm. We have previously found that monomers of the short sequence of Aβ (peptide 25-35) reduce sensory-induced theta oscillations. However, considering on the one hand that different Aβ sequences differentially affect hippocampal oscillations and on the other hand that Aβ oligomers seem to be responsible for the cognitive decline observed in AD, here we aimed to explore the effect of Aβ oligomers on sensory-induced theta rhythm. Our results show that intracisternal injection of Aβ1-42 oligomers, which has no significant effect on spontaneous hippocampal activity, disrupts the induction of theta rhythm upon sensory stimulation. Instead of increasing the power in the theta band, the hippocampus of Aβ-treated animals responds to sensory stimulation (tail pinch) with an increase in lower frequencies. These findings demonstrate that Aβ alters induced theta rhythm, providing an in vivo model to test for therapeutic approaches to overcome Aβ-induced hippocampal and cognitive dysfunctions.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2012 ","pages":"236289"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3350957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30631515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of endurance and resistance training on calcitonin gene-related Peptide and acetylcholine receptor at slow and fast twitch skeletal muscles and sciatic nerve in male wistar rats. 耐力和阻力训练对雄性wistar大鼠慢、快抽动骨骼肌和坐骨神经降钙素基因相关肽和乙酰胆碱受体的影响。

International Journal of Peptides Pub Date : 2012-01-01 Epub Date: 2012-06-18 DOI: 10.1155/2012/962651

Abdolhossein Parnow, Reza Gharakhanlou, Zeinab Gorginkaraji, Somayeh Rajabi, Rasoul Eslami, Mahdi Hedayati, Reza Mahdian

{"title":"Effects of endurance and resistance training on calcitonin gene-related Peptide and acetylcholine receptor at slow and fast twitch skeletal muscles and sciatic nerve in male wistar rats.","authors":"Abdolhossein Parnow, Reza Gharakhanlou, Zeinab Gorginkaraji, Somayeh Rajabi, Rasoul Eslami, Mahdi Hedayati, Reza Mahdian","doi":"10.1155/2012/962651","DOIUrl":"https://doi.org/10.1155/2012/962651","url":null,"abstract":"The aim of this study was to investigate effects of endurance and resistance training (ET and RT) on CGRP and AChRs at slow and fast twitch muscles and sciatic nerve in rats. Twenty-five male rats were randomly assigned into three groups including sedentary (SED), endurance training (ET), and resistance training (RT). Animals of ET exercised for 12 weeks, five times/week, and 60 min/day at 30 m/min. Animals of RT were housed in metal cage with 2 m high wire-mesh tower, with water bottles set at the top. 48 h after the last session of training protocol, animals were anaesthetized. The right sciatic nerves were removed; then, Soleus (SOL) and Tibialis anterior (TA) muscles were excised and immediately snap frozen in liquid nitrogen. All frozen tissues were stored at -80°C. Results showed that, after both ET and RT, CGRP content as well as AChR content of SOL and TA muscles significantly increased. But there was no significant difference among groups at sciatic nerve' CGRP content. In conclusion, data demonstrate that ET and RT lead to changes of CGRP and AChR content of ST and FT muscles. The changes indicate to the importance of neuromuscular activity.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2012 ","pages":"962651"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/962651","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30733738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Molecular Cloning and Sequence Analysis of the cDNAs Encoding Toxin-Like Peptides from the Venom Glands of Tarantula Grammostola rosea. 红狼蛛毒腺毒素样肽编码cdna的克隆与序列分析。

International Journal of Peptides Pub Date : 2012-01-01 Epub Date: 2012-02-29 DOI: 10.1155/2012/731293

Tadashi Kimura, Seigo Ono, Tai Kubo

{"title":"Molecular Cloning and Sequence Analysis of the cDNAs Encoding Toxin-Like Peptides from the Venom Glands of Tarantula Grammostola rosea.","authors":"Tadashi Kimura, Seigo Ono, Tai Kubo","doi":"10.1155/2012/731293","DOIUrl":"https://doi.org/10.1155/2012/731293","url":null,"abstract":"Tarantula venom glands produce a large variety of bioactive peptides. Here we present the identification of venom components obtained by sequencing clones isolated from a cDNA library prepared from the venom glands of the Chilean common tarantula, Grammostola rosea. The cDNA sequences of about 1500 clones out of 4000 clones were analyzed after selection using several criteria. Forty-eight novel toxin-like peptides (GTx1 to GTx7, and GTx-TCTP and GTx-CRISP) were predicted from the nucleotide sequences. Among these peptides, twenty-four toxins are ICK motif peptides, eleven peptides are MIT1-like peptides, and seven are ESTX-like peptides. Peptides similar to JZTX-64, aptotoxin, CRISP, or TCTP are also obtained. GTx3 series possess a cysteine framework that is conserved among vertebrate MIT1, Bv8, prokineticins, and invertebrate astakines. GTx-CRISP is the first CRISP-like protein identified from the arthropod venom. Real-time PCR revealed that the transcripts for TCTP-like peptide are expressed in both the pereopodal muscle and the venom gland. Furthermore, a unique peptide GTx7-1, whose signal and prepro sequences are essentially identical to those of HaTx1, was obtained.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2012 ","pages":"731293"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/731293","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30573948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Identification of histoplasma-specific peptides in human urine. 人尿中组织浆特异性肽的鉴定。

International Journal of Peptides Pub Date : 2012-01-01 Epub Date: 2012-03-26 DOI: 10.1155/2012/621329

David K Crockett, Mark M Kushnir, Joann L Cloud, Edward R Ashwood, Alan L Rockwood

{"title":"Identification of histoplasma-specific peptides in human urine.","authors":"David K Crockett, Mark M Kushnir, Joann L Cloud, Edward R Ashwood, Alan L Rockwood","doi":"10.1155/2012/621329","DOIUrl":"https://doi.org/10.1155/2012/621329","url":null,"abstract":"Histoplasmosis is a severe dimorphic fungus infection, which is often difficult to diagnose due to similarity in symptoms to other diseases and lack of specific diagnostic tests. Urine samples from histoplasma-antigen-positive patients and appropriate controls were prepared using various sample preparation strategies including immunoenrichment, ultrafiltration, high-abundant protein depletion, deglycosylation, reverse-phase fractions, and digest using various enzymes. Samples were then analyzed by nanospray tandem mass spectrometry. Accurate mass TOF scans underwent molecular feature extraction and statistical analysis for unique disease makers, and acquired MS/MS data were searched against known human and histoplasma proteins. In human urine, some 52 peptides from 37 Histoplasma proteins were identified with high confidence. This is the first report of identification of a large number of Histoplasma-specific peptides from immunoassay-positive patient samples using tandem mass spectrometry and bioinformatics techniques. These findings may lead to novel diagnostic markers for histoplasmosis in human urine.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":" ","pages":"621329"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/621329","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40185828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Pan-PPAR Agonist, Bezafibrate, Restores Angiogenesis in Hindlimb Ischemia in Normal and Diabetic Rats. 泛ppar激动剂贝扎布特恢复正常和糖尿病大鼠后肢缺血血管生成。

International Journal of Peptides Pub Date : 2012-01-01 Epub Date: 2012-06-03 DOI: 10.1155/2012/637212

M Khazaei, E Salehi, B Rashidi

{"title":"Pan-PPAR Agonist, Bezafibrate, Restores Angiogenesis in Hindlimb Ischemia in Normal and Diabetic Rats.","authors":"M Khazaei, E Salehi, B Rashidi","doi":"10.1155/2012/637212","DOIUrl":"https://doi.org/10.1155/2012/637212","url":null,"abstract":"Introduction. The aim of this study was to investigate the effect of bezafibrate as a pan-PPAR agonist on angiogenesis and serum nitrite, the main metabolite of nitric oxide (NO), vascular endothelial growth factor (VEGF) and VEGF receptor-2 (VEGFR-2) concentrations in hindlimb ischemia model of normal and type I diabetic rats. Methods. 28 male Wistar rats were divided into control and diabetic groups. Then, all rats underwent unilateral hindlimb ischemia. After recovery, they were randomly assigned to one of the following experimental groups: (1) control; (2) control + bezafibrate (400 mg/kg/day); (3) diabetic; (4) diabetic + beztafibrate. After three weeks, blood samples were taken and capillary density was evaluated in the gasterocnemius muscle of ischemic limb. Results. Bezafibrate increased capillary density and capillary/fiber ratio in ischemic leg of diabetic and control rats (P < 0.05). Serum VEGF and VEGFR-2 concentrations did not alter after bezafibrate administration, however, serum nitrite concentration was significantly higher in bezafibrate-treated groups than non-treated groups (P < 0.05). Discussion. It seems that bezafibrate, as a pan PPAR agonist, restores angiogenesis in hindlimb ischemic diabetic animals and is useful for prevention and/or treatment of peripheral artery disease in diabetic subjects.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2012 ","pages":"637212"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/637212","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30693383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

A meta-analysis of the therapeutic effects of glucagon-like Peptide-1 agonist in heart failure. 胰高血糖素样肽-1激动剂治疗心力衰竭疗效的荟萃分析。

International Journal of Peptides Pub Date : 2012-01-01 Epub Date: 2012-07-01 DOI: 10.1155/2012/249827

Mohammed Munaf, Pierpaolo Pellicori, Victoria Allgar, Kenneth Wong

{"title":"A meta-analysis of the therapeutic effects of glucagon-like Peptide-1 agonist in heart failure.","authors":"Mohammed Munaf, Pierpaolo Pellicori, Victoria Allgar, Kenneth Wong","doi":"10.1155/2012/249827","DOIUrl":"https://doi.org/10.1155/2012/249827","url":null,"abstract":"We conducted a meta-analysis of the existing literature of the therapeutic effects of using GLP-1 agonists to improve the metabolism of the failing heart. Animal studies showed significant improvement in markers of cardiac function, such as left ventricular ejection fraction (LVEF), with regular GLP-1 agonist infusions. In clinical trials, the potential effects of GLP-1 agonists in improving cardiac function were modest: LVEF improved by 4.4% compared to placebo (95% C.I 1.36-7.44, P = 0.005). However, BNP levels were not significantly altered by GLP-1 agonists in heart failure. In two trials, a modest increase in heart rate by up to 7 beats per minute was noted, but meta-analysis demonstrated this was not significant statistically. The small number of studies plus variation in the concentration and length of the regime between the trials would limit our conclusions, even though statistically, heterogeneity chi-squared tests did not reveal any significant heterogeneity in the endpoints tested. Moreover, studies in non-diabetics with heart failure yielded conflicting results. In conclusion, the use of GLP-1 agonists has at best a modest effect on ejection fraction improvement in heart failure, but there was no significant improvement in BNP levels in the meta-analysis.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2012 ","pages":"249827"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/249827","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30774773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Large Scale Solid Phase Synthesis of Peptide Drugs: Use of Commercial Anion Exchange Resin as Quenching Agent for Removal of Iodine during Disulphide Bond Formation. 多肽药物的大规模固相合成：使用商用阴离子交换树脂作为淬火剂去除二硫键形成过程中的碘。

International Journal of Peptides Pub Date : 2012-01-01 Epub Date: 2012-10-15 DOI: 10.1155/2012/323907

K M Bhaskara Reddy, Y Bharathi Kumari, Dokka Mallikharjunasarma, Kamana Bulliraju, Vanjivaka Sreelatha, Kuppanna Ananda

{"title":"Large Scale Solid Phase Synthesis of Peptide Drugs: Use of Commercial Anion Exchange Resin as Quenching Agent for Removal of Iodine during Disulphide Bond Formation.","authors":"K M Bhaskara Reddy, Y Bharathi Kumari, Dokka Mallikharjunasarma, Kamana Bulliraju, Vanjivaka Sreelatha, Kuppanna Ananda","doi":"10.1155/2012/323907","DOIUrl":"10.1155/2012/323907","url":null,"abstract":"The S-acetamidomethyl (Acm) or trityl (Trt) protecting groups are widely used in the chemical synthesis of peptides that contain one or more disulfide bonds. Treatment of peptides containing S-Acm protecting group with iodine results in simultaneous removal of the sulfhydryl protecting group and disulfide formation. However, the excess iodine needs to be quenched or adsorbed as quickly as possible after completion of the disulfide bond formation in order to minimize side reactions that are often associated with the iodination step. We report here a simple method for simultaneous quenching and removal of iodine and isolation of disulphide bridge peptides. The use of excess inexpensive anion exchange resin to the oxidized peptide from the aqueous acetic acid/methanol solution affords quantitative removal of iodine and other color impurities. This improves the resin life time of expensive chromatography media that is used in preparative HPLC column during the purification of peptide using preparative HPLC. Further, it is very useful for the conversion of TFA salt to acetate in situ. It was successfully applied commercially, to the large scale synthesis of various peptides including Desmopressin, Oxytocin, and Octreotide. This new approach offers significant advantages such as more simple utility, minimal side reactions, large scale synthesis of peptide drugs, and greater cost effectiveness.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2012 ","pages":"323907"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31022289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial peptides as infection imaging agents: better than radiolabeled antibiotics. 抗菌肽作为感染显像剂:优于放射性标记抗生素。

International Journal of Peptides Pub Date : 2012-01-01 Epub Date: 2012-05-17 DOI: 10.1155/2012/965238

Muammad Saeed Akhtar, Muhammad Babar Imran, Muhammad Afzal Nadeem, Abubaker Shahid

{"title":"Antimicrobial peptides as infection imaging agents: better than radiolabeled antibiotics.","authors":"Muammad Saeed Akhtar, Muhammad Babar Imran, Muhammad Afzal Nadeem, Abubaker Shahid","doi":"10.1155/2012/965238","DOIUrl":"https://doi.org/10.1155/2012/965238","url":null,"abstract":"Nuclear medicine imaging techniques offer whole body imaging for localization of number and site of infective foci inspite of limitation of spatial resolution. The innate human immune system contains a large member of important elements including antimicrobial peptides to combat any form of infection. However, development of antibiotics against bacteria progressed rapidly and gained popularity over antimicrobial peptides but even powerful antimicrobials failed to reduce morbidity and mortality due to emergence of mutant strains of bacteria resulting in antimicrobial resistance. Differentiation between infection and inflammation using radiolabeled compounds with nuclear medicine techniques has always been a dilemma which is still to be resolved. Starting from nonspecific tracers to specific radiolabeled tracers, the question is still unanswered. Specific radiolabeled tracers included antibiotics and antimicrobial peptides which bind directly to the bacteria for efficient localization with advanced nuclear medicine equipments. However, there are merits and demerits attributed to each. In the current paper, radiolabeled antibiotics and radiolabeled peptides for infection localization have been discussed starting with the background of primitive nonspecific tracers. Radiolabeled antimicrobial peptides have certain merits compared with labeled antibiotics which make them superior agents for localization of infective focus.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2012 ","pages":"965238"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/965238","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30673833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49