International Journal of Peptides最新文献_第3页

Structural Features of the Peptide Homologous to 6-25 Fragment of Influenza A PB1 Protein. 甲型流感PB1蛋白6-25片段同源肽的结构特征

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-12-24 DOI: 10.1155/2013/370832

Vladimir V Egorov, Oleg V Matusevich, Aram A Shaldzhyan, Alexey N Skvortsov, Yana A Zabrodskaya, Yuri P Garmay, Sergey B Landa, Dmitry V Lebedev, Vladimir V Zarubayev, Alexey K Sirotkin, Andrey V Vasin, Oleg I Kiselev

引用次数: 9

Nociceptin Signaling Involves a Calcium-Based Depolarization in Tetrahymena thermophila. 痛觉肽信号传导参与嗜热四膜虫钙基去极化。

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-04-29 DOI: 10.1155/2013/573716

Thomas Lampert, Cheryl Nugent, John Weston, Nathanael Braun, Heather Kuruvilla

{"title":"Nociceptin Signaling Involves a Calcium-Based Depolarization in Tetrahymena thermophila.","authors":"Thomas Lampert, Cheryl Nugent, John Weston, Nathanael Braun, Heather Kuruvilla","doi":"10.1155/2013/573716","DOIUrl":"https://doi.org/10.1155/2013/573716","url":null,"abstract":"Tetrahymena thermophila are free-living, ciliated eukaryotes. Their behavioral response to stimuli is well characterized and easily observable, since cells swim toward chemoattractants and avoid chemorepellents. Chemoattractant responses involve increased swim speed or a decreased change in swim direction, while chemorepellent signaling involves ciliary reversal, which causes the organism to jerk back and forth, swim in small circles, or spin in an attempt to get away from the repellent. Many food sources, such as proteins, are chemoattractants for these organisms, while a variety of compounds are repellents. Repellents in nature are thought to come from the secretions of predators or from ruptured organisms, which may serve as \"danger\" signals. Interestingly, several peptides involved in vertebrate pain signaling are chemorepellents in Tetrahymena, including substances P, ACTH, PACAP, VIP, and nociceptin. Here, we characterize the response of Tetrahymena thermophila to three different isoforms of nociceptin. We find that G-protein inhibitors and tyrosine kinase inhibitors do not affect nociceptin avoidance. However, the calcium chelator, EGTA, and the SERCA calcium ATPase inhibitor, thapsigargin, both inhibit nociceptin avoidance, implicating calcium in avoidance. This result is confirmed by electrophysiology studies which show that 50 μM nociceptin-NH2 causes a sustained depolarization of approximately 40 mV, which is eliminated by the addition of extracellular EGTA.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2013 ","pages":"573716"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/573716","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31481456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Phage Display Screening for Tumor Necrosis Factor- α -Binding Peptides: Detection of Inflammation in a Mouse Model of Hepatitis. 肿瘤坏死因子- α结合肽的噬菌体展示筛选:检测肝炎小鼠模型的炎症。

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-02-26 DOI: 10.1155/2013/348409

Coralie Sclavons, Carmen Burtea, Sébastien Boutry, Sophie Laurent, Luce Vander Elst, Robert N Muller

{"title":"Phage Display Screening for Tumor Necrosis Factor- α -Binding Peptides: Detection of Inflammation in a Mouse Model of Hepatitis.","authors":"Coralie Sclavons, Carmen Burtea, Sébastien Boutry, Sophie Laurent, Luce Vander Elst, Robert N Muller","doi":"10.1155/2013/348409","DOIUrl":"https://doi.org/10.1155/2013/348409","url":null,"abstract":"TNF- α is one of the most abundant cytokines produced in many inflammatory and autoimmune conditions such as multiple sclerosis, chronic hepatitis C, or neurodegenerative diseases. These pathologies remain difficult to diagnose and consequently difficult to treat. The aim of this work is to offer a new diagnostic tool by seeking new molecular probes for medical imaging. The target-specific part of the probe consists here of heptameric peptides selected by the phage display technology for their affinity for TNF- α . Several affinity tests allowed isolating 2 peptides that showed the best binding capacity to TNF- α . Finally, the best peptide was synthesized in both linear and cyclic forms and tested on the histological sections of concanavalin-A-(ConA-)treated mice liver. In this well-known hepatitis mouse model, the best results were obtained with the cyclic form of peptide 2, which allowed for the staining of inflamed areas in the liver. The cyclic form of peptide 2 (2C) was, thus, covalently linked to iron oxide nanoparticles (magnetic resonance imaging (MRI) contrast agent) and tested in the ConA-induced hepatitis mouse model. The vectorized nanoparticles allowed for the detection of inflammation as well as of the free peptide. These ex vivo results suggest that phage display-selected peptides can direct imaging contrast agents to inflammatory areas.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":" ","pages":"348409"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/348409","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40229272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Interaction between Pirenzepine and Ninjinto, a Traditional Japanese Herbal Medicine, on the Plasma Gut-Regulated Peptide Levels in Humans. 吡伦泽平与日本传统中草药忍冬对人血浆肠调节肽水平的相互作用。

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-03-27 DOI: 10.1155/2013/907850

Yuhki Sato, Itoh Hiroki, Yosuke Suzuki, Ryosuke Tatsuta, Masaharu Takeyama

{"title":"Interaction between Pirenzepine and Ninjinto, a Traditional Japanese Herbal Medicine, on the Plasma Gut-Regulated Peptide Levels in Humans.","authors":"Yuhki Sato, Itoh Hiroki, Yosuke Suzuki, Ryosuke Tatsuta, Masaharu Takeyama","doi":"10.1155/2013/907850","DOIUrl":"https://doi.org/10.1155/2013/907850","url":null,"abstract":"The Japanese herbal medicine (Kampo) Ninjinto has been used for the treatment of gastroenteritis, esogastritis, gastric atony, gastrectasis, vomiting, and anorexia. The pharmacological effects of Ninjinto on the gastrointestine are due to changes in the levels of gut-regulated peptide, such as motilin, somatostatin, calcitonin gene-related peptide (CGRP), substance P, and vasoactive intestinal polypeptide (VIP). The release of these peptides is controlled by acetylcholine (ACh) from the preganglionic fibers of the parasympathetic nerve. Thus, we examined the effects of the selective M1 muscarinic receptor antagonist pirenzepine on the elevation of Ninjinto-induced plasma the area under the plasma gut-regulated peptide concentration-time curve from 0 to 240 min (AUC0→240 min) in humans. Oral pretreatment with pirenzepine significantly reduced the Ninjinto-induced elevation of plasma motilin and substance P release (AUC0→240 min). Combined treatment with Ninjinto and pirenzepine significantly increased the release of plasma somatostatin (AUC0→240 min) compared with administration of Ninjinto alone or placebo. Ninjinto appeared to induce the release of substance P and motilin into plasma mainly through the activation of M1 muscarinic receptors, and pirenzepine may affect the pharmacologic action of Ninjinto by the elevation of plasma substance P, motilin, and somatostatin.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2013 ","pages":"907850"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/907850","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31467896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Peptide receptor targeting in cancer: the somatostatin paradigm. 肿瘤中的肽受体靶向:生长抑素范式。

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-02-07 DOI: 10.1155/2013/926295

Federica Barbieri, Adriana Bajetto, Alessandra Pattarozzi, Monica Gatti, Roberto Würth, Stefano Thellung, Alessandro Corsaro, Valentina Villa, Mario Nizzari, Tullio Florio

{"title":"Peptide receptor targeting in cancer: the somatostatin paradigm.","authors":"Federica Barbieri, Adriana Bajetto, Alessandra Pattarozzi, Monica Gatti, Roberto Würth, Stefano Thellung, Alessandro Corsaro, Valentina Villa, Mario Nizzari, Tullio Florio","doi":"10.1155/2013/926295","DOIUrl":"https://doi.org/10.1155/2013/926295","url":null,"abstract":"Peptide receptors involved in pathophysiological processes represent promising therapeutic targets. Neuropeptide somatostatin (SST) is produced by specialized cells in a large number of human organs and tissues. SST primarily acts as inhibitor of endocrine and exocrine secretion via the activation of five G-protein-coupled receptors, named sst1-5, while in central nervous system, SST acts as a neurotransmitter/neuromodulator, regulating locomotory and cognitive functions. Critical points of SST/SST receptor biology, such as signaling pathways of individual receptor subtypes, homo- and heterodimerization, trafficking, and cross-talk with growth factor receptors, have been extensively studied, although functions associated with several pathological conditions, including cancer, are still not completely unraveled. Importantly, SST exerts antiproliferative and antiangiogenic effects on cancer cells in vitro, and on experimental tumors in vivo. Moreover, SST agonists are clinically effective as antitumor agents for pituitary adenomas and gastro-pancreatic neuroendocrine tumors. However, SST receptors being expressed by tumor cells of various tumor histotypes, their pharmacological use is potentially extendible to other cancer types, although to date no significant results have been obtained. In this paper the most recent findings on the expression and functional roles of SST and SST receptors in tumor cells are discussed.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2013 ","pages":"926295"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/926295","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31295516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 103

Amyloid Beta peptides differentially affect hippocampal theta rhythms in vitro. 淀粉样β肽在体外对海马θ节律有不同影响。

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-06-25 DOI: 10.1155/2013/328140

Armando I Gutiérrez-Lerma, Benito Ordaz, Fernando Peña-Ortega

{"title":"Amyloid Beta peptides differentially affect hippocampal theta rhythms in vitro.","authors":"Armando I Gutiérrez-Lerma, Benito Ordaz, Fernando Peña-Ortega","doi":"10.1155/2013/328140","DOIUrl":"https://doi.org/10.1155/2013/328140","url":null,"abstract":"Soluble amyloid beta peptide (A β ) is responsible for the early cognitive dysfunction observed in Alzheimer's disease. Both cholinergically and glutamatergically induced hippocampal theta rhythms are related to learning and memory, spatial navigation, and spatial memory. However, these two types of theta rhythms are not identical; they are associated with different behaviors and can be differentially modulated by diverse experimental conditions. Therefore, in this study, we aimed to investigate whether or not application of soluble A β alters the two types of theta frequency oscillatory network activity generated in rat hippocampal slices by application of the cholinergic and glutamatergic agonists carbachol or DHPG, respectively. Due to previous evidence that oscillatory activity can be differentially affected by different A β peptides, we also compared Aβ 25-35 and Aβ 1-42 for their effects on theta rhythms in vitro at similar concentrations (0.5 to 1.0 μ M). We found that Aβ 25-35 reduces, with less potency than Aβ 1-42, carbachol-induced population theta oscillatory activity. In contrast, DHPG-induced oscillatory activity was not affected by a high concentration of Aβ 25-35 but was reduced by Aβ 1-42. Our results support the idea that different amyloid peptides might alter specific cellular mechanisms related to the generation of specific neuronal network activities, instead of exerting a generalized inhibitory effect on neuronal network function. ","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2013 ","pages":"328140"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/328140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31601074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Development of the schedule for multiple parallel "difficult" Peptide synthesis on pins. 制定在引脚上进行多个并行 "高难度 "多肽合成的时间表。

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-08-20 DOI: 10.1155/2013/197317

Ekaterina F Kolesanova, Maxim A Sanzhakov, Oleg N Kharybin

{"title":"Development of the schedule for multiple parallel \"difficult\" Peptide synthesis on pins.","authors":"Ekaterina F Kolesanova, Maxim A Sanzhakov, Oleg N Kharybin","doi":"10.1155/2013/197317","DOIUrl":"10.1155/2013/197317","url":null,"abstract":"Unified schedule for multiple parallel solid-phase synthesis of so-called \"difficult\" peptides on polypropylene pins was developed. Increase in the efficiency of 9-fluorenyl(methoxycarbonyl) N-terminal amino-protecting group removal was shown to have a greater influence on the accuracy of the \"difficult\" peptide synthesis than the use of more efficient amino acid coupling reagents such as aminium salts. Hence the unified schedule for multiple parallel solid-phase synthesis of \"difficult\" peptides included the procedure for N-terminal amino group deprotection modified by applying a more efficient reagent for the deprotection and the standard procedure of amino acid coupling by carbodiimide method with an additional coupling using aminium salts, if necessary. Amino acid coupling with the help of carbodiimide allows to follow the completeness of the coupling via the bromophenol blue indication, thus providing the accuracy of the synthesis and preventing an overexpenditure of expensive reagents. About 100 biotinylated hepatitis C virus envelope protein fragments, most of which represented \"difficult\" peptides, were successfully obtained by synthesis on pins with the help of the developed unified schedule. ","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2013 ","pages":"197317"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3762146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31724980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic ghrelin administration alters serum biomarkers of angiogenesis in diet-induced obese mice. 全身胃饥饿素给药改变饮食诱导的肥胖小鼠血管生成的血清生物标志物。

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-02-28 DOI: 10.1155/2013/249565

M Khazaei, Z Tahergorabi

{"title":"Systemic ghrelin administration alters serum biomarkers of angiogenesis in diet-induced obese mice.","authors":"M Khazaei, Z Tahergorabi","doi":"10.1155/2013/249565","DOIUrl":"https://doi.org/10.1155/2013/249565","url":null,"abstract":"Introduction. Ghrelin is a gastrointestinal endocrine peptide that was initially identified as the endogenous ligand of growth hormone secretagogue receptor; however, recently, the cardiovascular effect of this peptide has been indicated. In this study, we investigated the effect of ghrelin administration on serum biomarkers of angiogenesis including leptin, nitric oxide (NO), vascular endothelial growth factor (VEGF), and its soluble receptor (VEGF receptor 1 or sFlt-1) in control- and diet-induced obese mice. Methods. Male C57BL/6 mice were randomly divided into four groups, normal diet (ND) or control, ND + ghrelin, high-fat-diet (HFD) or obese and HFD + ghrelin (n = 6/group). Obese and control groups received either HFD or ND for 15 weeks. Then, the ghrelin was injected subcutaneously 100 µg/kg twice daily for 10 days. At the end of experiment, blood samples were collected for blood glucose, serum insulin, VEGF, sFlt-1, NO, and leptin measurements. Results. The obese animals had higher serum NO and leptin concentrations without changes in serum VEGF and sFlt-1 levels compared to control. Administration of ghrelin significantly increased serum VEGF and decreased serum leptin and NO concentrations in HFD group. Conclusion. Since ghrelin changes serum biomarkers of angiogenesis, it seems that it gets involved during states with abnormal angiogenesis.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":" ","pages":"249565"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/249565","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40229271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Antimicrobial lactoferrin peptides: the hidden players in the protective function of a multifunctional protein. 抗菌乳铁蛋白肽:隐藏在多功能蛋白保护功能中的玩家。

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-02-13 DOI: 10.1155/2013/390230

Mau Sinha, Sanket Kaushik, Punit Kaur, Sujata Sharma, Tej P Singh

引用次数: 104

High-throughput Peptide epitope mapping using carbon nanotube field-effect transistors. 利用碳纳米管场效应晶体管进行高通量肽表位定位。

International Journal of Peptides Pub Date : 2013-01-01 Epub Date: 2013-07-14 DOI: 10.1155/2013/849303

Steingrimur Stefansson, Martha Knight, Hena H Kwon, Lára A Stefansson, Saeyoung Nate Ahn

{"title":"High-throughput Peptide epitope mapping using carbon nanotube field-effect transistors.","authors":"Steingrimur Stefansson, Martha Knight, Hena H Kwon, Lára A Stefansson, Saeyoung Nate Ahn","doi":"10.1155/2013/849303","DOIUrl":"https://doi.org/10.1155/2013/849303","url":null,"abstract":"Label-free and real-time detection technologies can dramatically reduce the time and cost of pharmaceutical testing and development. However, to reach their full promise, these technologies need to be adaptable to high-throughput automation. To demonstrate the potential of single-walled carbon nanotube field-effect transistors (SWCNT-FETs) for high-throughput peptide-based assays, we have designed circuits arranged in an 8 × 12 (96-well) format that are accessible to standard multichannel pipettors. We performed epitope mapping of two HIV-1 gp160 antibodies using an overlapping gp160 15-mer peptide library coated onto nonfunctionalized SWCNTs. The 15-mer peptides did not require a linker to adhere to the non-functionalized SWCNTs, and binding data was obtained in real time for all 96 circuits. Despite some sequence differences in the HIV strains used to generate these antibodies and the overlapping peptide library, respectively, our results using these antibodies are in good agreement with known data, indicating that peptides immobilized onto SWCNT are accessible and that linear epitope mapping can be performed in minutes using SWCNT-FET.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2013 ","pages":"849303"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/849303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31667148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3