International Journal of Peptides最新文献

Amyloid β Peptide-Induced Changes in Prefrontal Cortex Activity and Its Response to Hippocampal Input β淀粉样蛋白肽诱导的前额叶皮层活动变化及其对海马输入的反应

International Journal of Peptides Pub Date : 2017-01-03 DOI: 10.1155/2017/7386809

Ernesto Flores-Martínez, F. Peña-Ortega

引用次数: 20

Peptide Inhibitor of Complement C1 Inhibits the Peroxidase Activity of Hemoglobin and Myoglobin. 补体C1肽抑制剂抑制血红蛋白和肌红蛋白过氧化物酶活性。

International Journal of Peptides Pub Date : 2017-01-01 Epub Date: 2017-09-10 DOI: 10.1155/2017/9454583

Pamela S Hair, Kenji M Cunnion, Neel K Krishna

{"title":"Peptide Inhibitor of Complement C1 Inhibits the Peroxidase Activity of Hemoglobin and Myoglobin.","authors":"Pamela S Hair, Kenji M Cunnion, Neel K Krishna","doi":"10.1155/2017/9454583","DOIUrl":"https://doi.org/10.1155/2017/9454583","url":null,"abstract":"Hemoglobin is the natural carrier of oxygen in red blood cells (RBCs). While intracellular hemoglobin provides life-sustaining oxygen transport, extracellular free hemoglobin displays toxicity due to inherent peroxidase activity generating reactive oxygen species that subsequently react with the hemoglobin molecule to produce toxic heme degradation products resulting in free radicals, oxidative stress damage, and lipid peroxidation. We have recently demonstrated that Peptide Inhibitor of Complement C1 (PIC1) inhibits peroxidase activity of the heme-based enzyme myeloperoxidase. To elucidate whether PIC1 could inhibit peroxidase activity of hemoglobin, we evaluated the consequence of PIC1 on RBC lysates, methemoglobin, and myoglobin using tetramethylbenzidine (TMB) as an oxidation target. PIC1 reversibly and dose-dependently prevented TMB oxidation to tetramethylbenzidine diimine by RBC lysates, methemoglobin, and myoglobin, having comparable activity to the inhibitor 4-aminobenzoic acid hydrazide. PIC1 inhibited TMB oxidation of RBC lysates similar to L-cysteine suggesting that the two cysteine residues contained in PIC1 may mediate peroxidase activity. PIC1 also inhibited heme destruction by NaOCl for RBC lysates, hemoglobin, and myoglobin as assayed by preservation of the Soret absorbance peak in the presence of NaOCl and reduction in free iron release. In conclusion, PIC1 inhibits peroxidase activity of hemoglobin and myoglobin likely via an antioxidant mechanism.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2017 ","pages":"9454583"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/9454583","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35502916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

A Synthetic Strategy for Conjugation of Paromomycin to Cell-Penetrating Tat(48-60) for Delivery and Visualization into Leishmania Parasites. Paromomycin与细胞穿透Tat(48-60)偶联的合成策略，用于利什曼原虫的递送和可视化。

International Journal of Peptides Pub Date : 2017-01-01 Epub Date: 2017-02-14 DOI: 10.1155/2017/4213037

Sira Defaus, Maria Gallo, María A Abengózar, Luis Rivas, David Andreu

引用次数: 8

The Dipeptides Ile-Tyr and Ser-Tyr Exert Distinct Effects on Catecholamine Metabolism in the Mouse Brainstem 二肽Ile-Tyr和Ser-Tyr对小鼠脑干儿茶酚胺代谢有明显影响

International Journal of Peptides Pub Date : 2016-02-11 DOI: 10.1155/2016/6020786

Kazuki Moriyasu, Takashi Ichinose, Akane Nakahata, Mitsuru Tanaka, T. Matsui, S. Furuya

引用次数: 7

Netrin-1 Peptide Is a Chemorepellent in Tetrahymena thermophila. 嗜热四膜虫中Netrin-1肽的化学驱避作用。

International Journal of Peptides Pub Date : 2016-01-01 Epub Date: 2016-03-31 DOI: 10.1155/2016/7142868

Heather Kuruvilla, Bradley Schmidt, Stephanie Song, Marian Bhajjan, Matthew Merical, Caleb Alley, Christopher Griffin, David Yoder, Josephine Hein, Daniel Kohl, Cambria Puffenberger, David Petroff, Elise Newcomer, Kortney Good, Graham Heston, Anna Hurtubise

{"title":"Netrin-1 Peptide Is a Chemorepellent in Tetrahymena thermophila.","authors":"Heather Kuruvilla, Bradley Schmidt, Stephanie Song, Marian Bhajjan, Matthew Merical, Caleb Alley, Christopher Griffin, David Yoder, Josephine Hein, Daniel Kohl, Cambria Puffenberger, David Petroff, Elise Newcomer, Kortney Good, Graham Heston, Anna Hurtubise","doi":"10.1155/2016/7142868","DOIUrl":"https://doi.org/10.1155/2016/7142868","url":null,"abstract":"Netrin-1 is a highly conserved, pleiotropic signaling molecule that can serve as a neuronal chemorepellent during vertebrate development. In vertebrates, chemorepellent signaling is mediated through the tyrosine kinase, src-1, and the tyrosine phosphatase, shp-2. Tetrahymena thermophila has been used as a model system for chemorepellent signaling because its avoidance response is easily characterized under a light microscope. Our experiments showed that netrin-1 peptide is a chemorepellent in T. thermophila at micromolar concentrations. T. thermophila adapts to netrin-1 over a time course of about 10 minutes. Netrin-adapted cells still avoid GTP, PACAP-38, and nociceptin, suggesting that netrin does not use the same signaling machinery as any of these other repellents. Avoidance of netrin-1 peptide was effectively eliminated by the addition of the tyrosine kinase inhibitor, genistein, to the assay buffer; however, immunostaining using an anti-phosphotyrosine antibody showed similar fluorescence levels in control and netrin-1 exposed cells, suggesting that tyrosine phosphorylation is not required for signaling to occur. In addition, ELISA indicates that a netrin-like peptide is present in both whole cell extract and secreted protein obtained from Tetrahymena thermophila. Further study will be required in order to fully elucidate the signaling mechanism of netrin-1 peptide in this organism. ","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2016 ","pages":"7142868"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/7142868","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34437953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mystixin-7 Peptide Protects Ionotropic Glutamatergic Mechanisms against Glutamate-Induced Excitotoxicity In Vitro. 神秘素-7肽保护嗜离子性谷氨酸机制对抗谷氨酸诱导的体外兴奋毒性。

International Journal of Peptides Pub Date : 2016-01-01 Epub Date: 2016-07-18 DOI: 10.1155/2016/5151843

Anatoly A Mokrushin

{"title":"Mystixin-7 Peptide Protects Ionotropic Glutamatergic Mechanisms against Glutamate-Induced Excitotoxicity In Vitro.","authors":"Anatoly A Mokrushin","doi":"10.1155/2016/5151843","DOIUrl":"https://doi.org/10.1155/2016/5151843","url":null,"abstract":"Hyperactivation of the N-methyl-D-aspartic acid type glutamate receptors (NMDARs) causes glutamate excitotoxicity, a process potentially important for many neurological diseases. This study aims to investigate protective effects of the synthetic corticotrophin-releasing factor-like peptide, mystixin-7 (MTX), on model glutamate-induced excitotoxicity in vitro. The technique online monitoring of electrophysiological parameters (excitatory glutamatergic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic (AMPAR) and NMDAR-dependent postsynaptic mechanisms) in the olfactory cortex slices was used. Application of L-glutamate in toxic concentration (20 mM) on slices evoked hyperactivation of NMDARs and weaker activation of the AMPARs. Upon further action agonist, the excessive activation of glutamate receptors was replaced by their irreversible blockade. Pretreatment of the slices using MTX in different concentrations (50 and 100 mg/mL) protected both NMDARs and AMPARs from glutamate-induced damage. An enzymatic treatment of MTX reduced hyperactivation of both NMDARs and AMPARs. The present study demonstrated that MTX minipeptide protected the functioning of both NMDARs and AMPARs against glutamate-induced damage. The MTX peptide is a prospective candidate for elaborated medication in treatment of neurological diseases. ","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2016 ","pages":"5151843"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34742429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

High Proteolytic Resistance of Spider-Derived Inhibitor Cystine Knots 蜘蛛衍生抑制剂胱氨酸结的高蛋白水解抗性

International Journal of Peptides Pub Date : 2015-12-30 DOI: 10.1155/2015/537508

K. Kikuchi, Mika Sugiura, Tadashi Kimura

{"title":"High Proteolytic Resistance of Spider-Derived Inhibitor Cystine Knots","authors":"K. Kikuchi, Mika Sugiura, Tadashi Kimura","doi":"10.1155/2015/537508","DOIUrl":"https://doi.org/10.1155/2015/537508","url":null,"abstract":"Proteolytic stability in gastrointestinal tract and blood plasma is the major obstacle for oral peptide drug development. Inhibitor cystine knots (ICKs) are linear cystine knot peptides which have multifunctional properties and could become promising drug scaffolds. ProTx-I, ProTx-II, GTx1-15, and GsMTx-4 were spider-derived ICKs and incubated with pepsin, trypsin, chymotrypsin, and elastase in physiological conditions to find that all tested peptides were resistant to pepsin, and ProTx-II, GsMTx-4, and GTx1-15 showed resistance to all tested proteases. Also, no ProTx-II degradation was observed in rat blood plasma for 24 hours in vitro and ProTx-II concentration in circulation decreased to half in 40 min, indicating absolute stability in plasma and fast clearance from the system. So far, linear peptides are generally thought to be unsuitable in vivo, but all tested ICKs were not degraded by pepsin and stomach could be selected for the alternative site of drug absorption for fast onset of the drug action. Since spider ICKs are selective inhibitors of various ion channels which are related to the pathology of many diseases, engineered ICKs will make a novel class of peptide medicines which can treat variety of bothering symptoms.","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90821311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

In silico conformational analysis of the short-sequence hypomurocin a peptides. 短序列亚黑素a肽的计算机构象分析。

International Journal of Peptides Pub Date : 2015-01-01 Epub Date: 2015-01-28 DOI: 10.1155/2015/281065

Zoltán Násztor, János Horváth, Balázs Leitgeb

{"title":"In silico conformational analysis of the short-sequence hypomurocin a peptides.","authors":"Zoltán Násztor, János Horváth, Balázs Leitgeb","doi":"10.1155/2015/281065","DOIUrl":"https://doi.org/10.1155/2015/281065","url":null,"abstract":"In this theoretical study, a conformational analysis was performed on short-sequence hypomurocin A peptides, in order to identify their characteristic structural properties. For each hypomurocin A molecule, not only the backbone conformations, but also the side-chain conformations were examined. The results indicated that certain tetrapeptide units could be characterized by types I and III β-turn structures, and considering the helical conformations, it could be concluded that the hypomurocin A peptides showed a preference for the 310-helical structure over the α-helical structure. Beside the backbone conformations, the side-chain conformations were investigated, and the preferred rotamer states of the side-chains of amino acids were determined. Furthermore, the occurrence of i ← i + 3 and i ← i + 4 intramolecular H-bonds was studied, which could play a role in the structural stabilization of β-turns and helical conformations. On the whole, our theoretical study supplied a comprehensive characterization of the three-dimensional structure of short-sequence hypomurocin A peptides. ","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2015 ","pages":"281065"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/281065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33399650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Proteins of Bartonella bacilliformis: Candidates for Vaccine Development. 巴通体杆菌的蛋白质:疫苗开发的候选物。

International Journal of Peptides Pub Date : 2015-01-01 Epub Date: 2015-08-30 DOI: 10.1155/2015/702784

Cesar Henriquez-Camacho, Palmira Ventosilla, Michael F Minnick, Joaquim Ruiz, Ciro Maguiña

引用次数: 18

Correlation between Saliva and Plasma Levels of Endothelin Isoforms ET-1, ET-2, and ET-3. 唾液和血浆内皮素异构体ET-1、ET-2和ET-3水平的相关性

International Journal of Peptides Pub Date : 2015-01-01 Epub Date: 2015-04-20 DOI: 10.1155/2015/828759

Roma Gurusankar, Prem Kumarathasan, Anusha Saravanamuthu, Errol M Thomson, Renaud Vincent

{"title":"Correlation between Saliva and Plasma Levels of Endothelin Isoforms ET-1, ET-2, and ET-3.","authors":"Roma Gurusankar, Prem Kumarathasan, Anusha Saravanamuthu, Errol M Thomson, Renaud Vincent","doi":"10.1155/2015/828759","DOIUrl":"https://doi.org/10.1155/2015/828759","url":null,"abstract":"Although saliva endothelins are emerging as valuable noninvasive cardiovascular biomarkers, reports on the relationship between isoforms in saliva and plasma remain scarce. We measured endothelins in concurrent saliva and plasma samples (n = 30 males; age 18-63) by HPLC-fluorescence. Results revealed statistically significant positive correlations among all isoforms between saliva and plasma: big endothelin-1 (BET-1, 0.55 ± 0.27 versus 3.35 ± 1.28 pmol/mL; r = 0.38, p = 0.041), endothelin-1 (ET-1, 0.52 ± 0.21 versus 3.45 ± 1.28 pmol/mL; r = 0.53, p = 0.003), endothelin-2 (ET-2, 0.21 ± 0.07 versus 1.63 ± 0.66 pmol/mL; r = 0.51, p = 0.004), and endothelin-3 (ET-3, 0.39 ± 0.19 versus 2.32 ± 1.44 pmol/mL; r = 0.75, p < 0.001). Correlations of BET-1, ET-1, and ET-3 within each compartment were positive in both plasma (p < 0.05) and saliva (p ≤ 0.1), whereas ET-2 was not significantly correlated with other isoforms in either plasma or saliva. For all isoforms, concentrations varied on average fivefold between individuals (90th/10th percentiles); individuals with high plasma endothelin levels generally had high saliva endothelin levels. Our results reveal that salivary ET isoform profiles portray the plasmatic profiles and support the view of coordinated regulation of ET-1 and ET-3, but distinct regulatory pathways for ET-2. ","PeriodicalId":14239,"journal":{"name":"International Journal of Peptides","volume":"2015 ","pages":"828759"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/828759","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33302761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5