International Journal of Neuroscience最新文献_第9页

Effect of pyridoxine or cobalamin supplementation on apoptosis and cell cycle progression in a human glioblastoma cell line. 补充吡哆醇或钴胺素对人胶质母细胞瘤细胞系凋亡和细胞周期进展的影响。

IF 1.7 4区医学

International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-10-03 DOI: 10.1080/00207454.2023.2263815

Carolina A Martínez-Mendiola, José A Estrada, Luis Á Zapi-Colín, Gerson G Contreras-Chávez, Irazú Contreras

{"title":"Effect of pyridoxine or cobalamin supplementation on apoptosis and cell cycle progression in a human glioblastoma cell line.","authors":"Carolina A Martínez-Mendiola, José A Estrada, Luis Á Zapi-Colín, Gerson G Contreras-Chávez, Irazú Contreras","doi":"10.1080/00207454.2023.2263815","DOIUrl":"10.1080/00207454.2023.2263815","url":null,"abstract":"Glioblastoma is the most aggressive type of brain tumor, with current therapies failing to significantly improve patient survival. Vitamins have important effects on cellular processes that are relevant for tumor development and progression.Aim: The present study explored the effect of pyridoxine or cobalamin supplementation on the viability and cell cycle progression of human glioblastoma cell line U-87 MG.Method: Cell cultures were treated with increasing concentrations of pyridoxine or cobalamin for 24-72 h. After supplementation, cell viability and cell cycle progression were assessed by spectrophotometry and flow cytometry. Analysis of Bcl-2 and active caspase 3 expression in supplemented cells was performed by western blot.Result: The results show that pyridoxine supplementation decreases cell viability in a dose and time dependent manner. Loss of viability in pyridoxin-supplemented cells is probably related to less cell cycle progression, higher active caspase 3 expression and apoptosis. In addition, Bcl-2 expression did not appear to be altered by vitamin supplementation, but active caspase 3 expression was significantly increased in pyridoxine-, but not cobalamin-supplemented cells, furthermore, cobalamin inhibited the pyridoxine cytotoxicity in the cell viability assay when combined.Conclusion: The results suggest that pyridoxine supplementation promotes apoptosis in human glioblastoma-derived cells and may be useful to enhance the effect of cytotoxic therapies in vivo.","PeriodicalId":14161,"journal":{"name":"International Journal of Neuroscience","volume":" ","pages":"1320-1331"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41130294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactome analysis implicates class II transactivator (CIITA) in depression and other neuroinflammatory disorders. 相互作用分析提示II类反式激活因子（CIITA）在抑郁症和其他神经炎性疾病中的作用。

IF 1.7 4区医学

International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-11-17 DOI: 10.1080/00207454.2023.2279502

Kishore Nagasubramanian, Krishnakant Gupta

{"title":"Interactome analysis implicates class II transactivator (CIITA) in depression and other neuroinflammatory disorders.","authors":"Kishore Nagasubramanian, Krishnakant Gupta","doi":"10.1080/00207454.2023.2279502","DOIUrl":"10.1080/00207454.2023.2279502","url":null,"abstract":"Purpose: Inappropriate inflammatory responses within the nervous system (neuroinflammation) have been implicated in several neurological conditions. Class II transactivator (CIITA), a principal regulator of the major histocompatibility complex II (MHCII), is known to play essential roles in inflammation. Hence, CIITA and its interactors could be potentially involved in multiple neurological disorders. However, the molecular mechanisms underlying CIITA-mediated neuroinflammation (NI) are yet to be understood.Materials and methods: In this regard, we analyzed the potential involvement of CIITA and its interactome in the regulation of neuroinflammation. In the present study, using various computational tools, we aimed (1) to identify NI-related proteins, (2) to filter the critical interactors in the CIITA-NI network, and (3) to analyze the protein-disease interactions and the associated molecular pathways through which CIITA could influence neuroinflammation.Results: CIITA was found to interact with P T GS2, GSK3B, and NR3C1 and may influence depressive disorders. Further, the IL4/IL13 pathway was found to be potentially underlying the CIITA-interactomemediated effects on neurological disorders. Moreover, CIITA was found to be connected to genes associated with depressive disorder through IL4, wherein CIITA was found to be potentially involved in depressive disorders through IL-4/IL-13 and hippo pathways. However, the present study is based on the existing data on protein interactomes and could be re-evaluated as newer interactions are discovered. Also, the functional mechanisms of CIITA's roles in neuroinflammation must be evaluated further.Conclusion: Notwithstanding these limitations, the results presented here, could form a basis for further experimental studies to assess CIITA as a potential therapeutic target in managing depression and other neuroinflammatory disorders.","PeriodicalId":14161,"journal":{"name":"International Journal of Neuroscience","volume":" ","pages":"1153-1171"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71481423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into the advances in therapeutic drugs for neuroinflammation-related diseases. 对神经炎症相关疾病治疗药物进展的见解。

IF 1.7 4区医学

International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-09-21 DOI: 10.1080/00207454.2023.2260088

Bozhi Luo

{"title":"Insights into the advances in therapeutic drugs for neuroinflammation-related diseases.","authors":"Bozhi Luo","doi":"10.1080/00207454.2023.2260088","DOIUrl":"10.1080/00207454.2023.2260088","url":null,"abstract":"Studies have shown that neurodegenerative diseases such as AD and PD are related to neuroinflammation. Neuroinflammation is a common inflammatory condition that can lead to a variety of dysfunction in the body. At present, it is no medications specifically approved to prevent or cure neuroinflammation, so even though many drugs can temporarily control the neurological symptoms of neuroinflammation, but no one can reverse the progress of neuroinflammation, let al.one completely cure neuroinflammation. Therefore, it is urgent to develop new drug development for neuroinflammation treatment. In this review, we highlight the therapeutic advancement in the field of neurodegenerative disorders, by focusing on the impact of neuroinflammation treatment has on these conditions, and the effective drugs for the treatment of neuroinflammation and neurodegenerative diseases and their latest research progress are reviewed according to the related signaling pathway, as well as the prospect of their clinical application is also discussed. The purpose of this review is to enable specialists to better understand the mechanisms underlying neuroinflammation and anti-inflammatory drugs, promote the development of therapeutic drugs for neuroinflammation and neurodegenerative diseases, and further provide therapeutic references for clinical neurologists.","PeriodicalId":14161,"journal":{"name":"International Journal of Neuroscience","volume":" ","pages":"1256-1281"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10656877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy and safety of intravenous thrombolytic therapy in patients over eighty years. 静脉溶栓治疗80岁以上患者的疗效和安全性。

IF 1.7 4区医学

International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-10-26 DOI: 10.1080/00207454.2023.2260086

Vedat Ali Yürekli, Burak Gürel, Nihat Şengeze, Mustafa Adanır, Cengiz Baloğlu

{"title":"Efficacy and safety of intravenous thrombolytic therapy in patients over eighty years.","authors":"Vedat Ali Yürekli, Burak Gürel, Nihat Şengeze, Mustafa Adanır, Cengiz Baloğlu","doi":"10.1080/00207454.2023.2260086","DOIUrl":"10.1080/00207454.2023.2260086","url":null,"abstract":"Introduction: Intravenous thrombolytic therapy (IVTT) is licensed for patients under 80 years in many countries. In this study, we aimed to demonstrate functional results and complication rates of IVTT in patients over eighty years and whether there is a difference in efficacy and safety between low dose and standard dose recombinant tissue plasminogen activator (rTPA).Methods: A retrospective observational study of patients over eighty who admitted to Suleyman Demirel University Faculty of Medicine Hospital between August 2016 and April 2021 and to Isparta City Hospital between April 2017 and April 2021 and diagnosed with acute ischemic stroke were conducted. Third month modified rankin scores (mRS) and mortality rates of patients and hemorrhagic transformations were determined.Results: There were 29 patients in IVTT group and 25 patients in non-IVTT group. By the third month, it was observed that functional independence (mRS 0-2) ratio was increased more in IVTT group, but it wasn't statistically significant (p: 0.087). In mortality and symptomatic intracerebral hemorrhage rates, there wasn't statistically significant difference between IVTT and non-IVTT groups and low dose and standard dose rTPA groups by the third month.Conclusion: The efficacy and complication rates of IVTT in patients over 80 years were found similar to not receive IVTT. These results support the safety of IVTT in patients over 80 years. In low or standard dose rTPA preference, we observed that there was not statistically significance in efficacy and safety. We believe that these results will be supported by studies with larger number of patients.","PeriodicalId":14161,"journal":{"name":"International Journal of Neuroscience","volume":" ","pages":"1250-1255"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracranial internal carotid aneurysm treated with a covered stent in an infant. 应用覆膜支架治疗婴儿颅外颈内动脉瘤。

IF 1.7 4区医学

International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-11-09 DOI: 10.1080/00207454.2023.2277665

David Dimitris Chlorogiannis, Athina Maria Aloizou, Theodore Mavridis, Angeliki Gkaifyllia, Maria Politi, Wolfgang Reith, Panagiotis Papanagiotou

引用次数: 0

Decreased anxiety-like behavior in a selectively bred high nicotine-preferring rat line. 选择性繁殖的高尼古丁偏好大鼠系中焦虑样行为的减少。

IF 1.7 4区医学

International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-11-15 DOI: 10.1080/00207454.2023.2279505

Merve Bayoglu, Meliha Ozturk Bintepe, Lutfiye Kanit, Burcu Balkan, Oguz Gozen, Ersin O Koylu, Aysegul Keser

{"title":"Decreased anxiety-like behavior in a selectively bred high nicotine-preferring rat line.","authors":"Merve Bayoglu, Meliha Ozturk Bintepe, Lutfiye Kanit, Burcu Balkan, Oguz Gozen, Ersin O Koylu, Aysegul Keser","doi":"10.1080/00207454.2023.2279505","DOIUrl":"10.1080/00207454.2023.2279505","url":null,"abstract":"Genetic vulnerability contributes significantly to the individual variability observed in nicotine dependence. Selective breeding for sensitivity to a particular effect of abused drugs has produced rodent lines useful for studying genetic vulnerability to drug addiction. Previous research showed that anxiety-related personality traits are associated with nicotine dependence. Therefore, we examined the differences in anxiety-like behavior between a high nicotine-preferring rat line and their controls. At the beginning of the study, all rats, naïve to any drug, were exposed sequentially to open field arena, marble-burying and elevated plus-maze paradigms. In the second step, all rats received nicotine in drinking water for 7 weeks. Behavioral tests were rerun on the final 2 weeks of chronic nicotine treatment. Elevated plus-maze testings under basal condition and during chronic nicotine treatment showed that the time spent on the open arms, preference for being in the open arms, and the latency to enter the closed arms were higher, whereas open arm avoidance index was lower in nicotine-preferring rats compared to the controls. In the open field test, nicotine-preferring rats spent longer time in the central zone and excreted less fecal pellets; they buried less marbles in the marble-burying test. These findings indicate a lower level of anxiety-like behavior in nicotine-preferring rat line under basal conditions and during chronic nicotine treatment. We conclude that lower anxiety level in nicotine-preferring rat line is consistent with novelty-seeking personality type and may increase vulnerability to nicotine dependence in this rat line.","PeriodicalId":14161,"journal":{"name":"International Journal of Neuroscience","volume":" ","pages":"1403-1413"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71481419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding corticomotor mechanisms for activation of non-target muscles during unilateral isometric contractions of leg muscles after stroke. 了解卒中后腿部肌肉单侧等长收缩过程中非靶肌肉激活的皮质运动机制。

IF 1.7 4区医学

International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-10-03 DOI: 10.1080/00207454.2023.2263817

Brice T Cleland, Matt Giffhorn, Arun Jayaraman, Sangeetha Madhavan

{"title":"Understanding corticomotor mechanisms for activation of non-target muscles during unilateral isometric contractions of leg muscles after stroke.","authors":"Brice T Cleland, Matt Giffhorn, Arun Jayaraman, Sangeetha Madhavan","doi":"10.1080/00207454.2023.2263817","DOIUrl":"10.1080/00207454.2023.2263817","url":null,"abstract":"Purpose: Muscle activation often occurs in muscles ipsilateral to a voluntarily activated muscle and to a greater extent after stroke. In this study, we measured muscle activation in non-target, ipsilateral leg muscles and used transcranial magnetic stimulation (TMS) to provide insight into whether corticomotor pathways contribute to involuntary activation.Materials and methods: Individuals with stroke performed unilateral isometric ankle dorsiflexion, ankle plantarflexion, knee extension, and knee flexion. To quantify involuntary muscle activation in non-target muscles, muscle activation was measured during contractions from the ipsilateral tibialis anterior (TA), medial gastrocnemius (MG), rectus femoris (RF), and biceps femoris (BF) and normalized to resting muscle activity. To provide insight into mechanisms of involuntary non-target muscle activation, TMS was applied to the contralateral hemisphere, and motor evoked potentials (MEPs) were recorded.Results: We found significant muscle activation in nearly every non-target muscle during isometric unilateral contractions. MEPs were frequently observed in non-target muscles, but greater non-target MEP amplitude was not associated with greater non-target muscle activation.Conclusions: Our results suggest that non-target muscle activation occurs frequently in individuals with chronic stroke. The lack of association between non-target TMS responses and non-target muscle activation suggests that non-target muscle activation may have a subcortical or spinal origin. Non-target muscle activation has important clinical implications because it may impair torque production, out-of-synergy movement, and muscle activation timing.","PeriodicalId":14161,"journal":{"name":"International Journal of Neuroscience","volume":" ","pages":"1332-1341"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10963339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41115925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety and effectiveness of nusinersen, a treatment for spinal muscular atrophy, in 524 patients: results from an interim analysis of post-marketing surveillance in Japan. 524例脊髓性肌萎缩症治疗药物nusinersen的安全性和有效性：来自日本上市后监测的中期分析结果

IF 1.7 4区医学

International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-09-07 DOI: 10.1080/00207454.2023.2251662

Yosuke Tachibana, Ryusuke Sato, Haruki Makioka, Misuzu Hoshino, Mingshou Jin

{"title":"Safety and effectiveness of nusinersen, a treatment for spinal muscular atrophy, in 524 patients: results from an interim analysis of post-marketing surveillance in Japan.","authors":"Yosuke Tachibana, Ryusuke Sato, Haruki Makioka, Misuzu Hoshino, Mingshou Jin","doi":"10.1080/00207454.2023.2251662","DOIUrl":"10.1080/00207454.2023.2251662","url":null,"abstract":"Purpose: Nusinersen is an antisense oligonucleotide approved for the treatment of spinal muscular atrophy (SMA). A post-marketing surveillance (PMS) has been ongoing (August 2017-August 2025) in all patients in Japan who were administered nusinersen intrathecally in real-world clinical settings. We report the interim analysis results for safety and effectiveness.Methods: This interim analysis was conducted using data collected from 524 patients whose case report forms were obtained at least once by May 30, 2022. Collected data included patient demographics and adverse events (AEs) for safety, and motor function assessments and Clinical Global Impressions of Improvement (CGI-I) for effectiveness.Results: Of the 524 patients in the safety analysis set, 522 patients who were diagnosed with SMA were included in the effectiveness analysis (infantile-onset SMA [n = 153, 29.3%], later-onset SMA [n = 369, 70.7%]). The median duration of treatment was 785.0 (range 1-1549) days. AEs occurred in 35.9% of patients (49.0% in infantile-onset SMA and 30.6% in later-onset SMA). Nusinersen treatment significantly improved Hammersmith Infant Neurological Examination scores in patients with infantile-onset SMA and Hammersmith Functional Motor Scale-Expanded scores in patients with later-onset SMA for up to nearly 3 years. Based on CGI-I assessments, 98.5-100% of patients receiving nusinersen 'improved' or remain 'unchanged'.Conclusions: This interim analysis of the large-scale, all-case PMS in patients who were administered nusinersen in Japan supports the safety and effectiveness of nusinersen. The benefit-risk balance of nusinersen treatment remains favorable.","PeriodicalId":14161,"journal":{"name":"International Journal of Neuroscience","volume":" ","pages":"1185-1197"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10165894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of 11 variants of the dopaminergic and cognitive pathways genes with major depression, schizophrenia and bipolar disorder in the Pakistani population. 巴基斯坦人群中11种多巴胺能和认知途径基因变异与重度抑郁症、精神分裂症和双相情感障碍的关联

IF 1.7 4区医学

International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-12-04 DOI: 10.1080/00207454.2023.2251661

Aisha Nasir Hashmi, Merlyn Sabina Raja, Rizwan Taj, Raees Ahmed Dharejo, Zehra Agha, Raheel Qamar, Maleeha Azam

{"title":"Association of 11 variants of the dopaminergic and cognitive pathways genes with major depression, schizophrenia and bipolar disorder in the Pakistani population.","authors":"Aisha Nasir Hashmi, Merlyn Sabina Raja, Rizwan Taj, Raees Ahmed Dharejo, Zehra Agha, Raheel Qamar, Maleeha Azam","doi":"10.1080/00207454.2023.2251661","DOIUrl":"10.1080/00207454.2023.2251661","url":null,"abstract":"Background: The dopaminergic pathways control neural signals that modulate mood and behaviour along and have a vital role in the aetiology of major depression (MDD), schizophrenia (SHZ) and bipolar disorder (BD). Genome-wide association studies (GWAS) have reported several dopaminergic and cognitive pathway genes association with these disorders however, no such comprehensive data was available regarding the Pakistani population.Objective: The present study was conducted to analyse the 11 genetic variants of dopaminergic and cognitive system genes in MDD, SHZ, and BD in the Pakistani population.Methods: A total of 1237 subjects [MDD n = 479; BD n = 222; SHZ n = 146; and controls n = 390], were screened for 11 genetic variants through polymerase chain reaction (PCR) techniques. Univariant followed by multivariant logistic regression analysis was applied to determine the genetic association.Results: Significant risk associations were observed for rs4532 and rs1799732 with MDD; and rs1006737 and rs2238056 with BD. However, after applying multiple test corrections rs4532 and rs1799732 association did not remain significant for MDD. Moreover, a protective association was found for three variants; DRD4-120bp, rs10033951 and rs2388334 in the current cohort.Conclusions: The present study revealed the risk association of single nucleotide polymorphisms (SNPs) rs1006737 and rs2238056 with BD and the protective effect of the DRD4-120bp variant in MDD and BD, of rs2388334 in BD and of rs10033951 in MDD, BD, and SHZ in the current Pakistani cohort. Thus, the study is valuable in understanding the genetic basis of MDD, BD and SHZ in the Pakistani population, which may pave the way for future functional studies.","PeriodicalId":14161,"journal":{"name":"International Journal of Neuroscience","volume":" ","pages":"1172-1184"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10109388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of donepezil hydrochloride on the transgenic Drosophila expressing human Aβ-42. 盐酸多奈哌齐对表达人Aβ-42的转基因果蝇的影响。

IF 1.7 4区医学

International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-11-21 DOI: 10.1080/00207454.2023.2262109

Yasir Hasan Siddique, Falaq Naz, Rahul, Himanshi Varshney, Mantasha Idrisi, M Shahid

{"title":"Effect of donepezil hydrochloride on the transgenic Drosophila expressing human Aβ-42.","authors":"Yasir Hasan Siddique, Falaq Naz, Rahul, Himanshi Varshney, Mantasha Idrisi, M Shahid","doi":"10.1080/00207454.2023.2262109","DOIUrl":"10.1080/00207454.2023.2262109","url":null,"abstract":"Aim: In the present study, the effect of donepezil hydrochloride was studied on the transgenic Drosophila expressing human amyloid beta-42 in the neurons.Methods: Donepezil hydrochloride at final concentration of 0.1, 1 and 10 mM was mixed in the diet and the flies expressing human amyloid beta-42 under Upstream Activation Sequence control (Alzheimer Disease [AD] flies) were allowed to feed on it for 30 days.Results: The AD flies exposed to various doses of Donepezil hydrochloride showed a dose dependent significant delay in the loss of climbing ability, increase in activity, reduction in the oxidative stress and apoptotic markers. A significant improvement was also observed in cognitive parameters. A dose dependent significant reduction in the activity of acetylcholinesterase was also observed. The docking studies suggest the positive interaction between donepezil, amyloid beta-42 and acetylcholinesterase. The results obtained from immunohistochemistry also showed a dose dependent significant reduction in the amyloid beta-42 aggregates.Conclusion: The results suggest that donepezil hydrochloride is potent enough to reduce the AD symptoms being mimicked in transgenic flies.","PeriodicalId":14161,"journal":{"name":"International Journal of Neuroscience","volume":" ","pages":"1293-1308"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41125972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0