International Journal of Molecular and Cellular Medicine最新文献

{"title":"Prenatal Diagnosis of Triploidy in Fetus with Unexpected Chromosomal Translocation of Maternal Origin.","authors":"Ajinkya Jadhav, Yamini Jadhav, Vidya Bhairi, Rukaiya Ansari, Premkumar Torane, Krutika Patil","doi":"10.22088/IJMCM.BUMS.12.1.81","DOIUrl":"10.22088/IJMCM.BUMS.12.1.81","url":null,"abstract":"<p><p>Triploidy is a lethal chromosomal abnormality. Fetuses with triploid condition have a tendency to die in early conception and very few survive to term. In this study, we report the prenatal diagnosis of fetal triploidy with unexpected chromosomal translocation. A 27 years old women was referred to our clinical cytogenetic department due to history of previous conceptus with intrauterine growth retardation at 21-22 weeks of gestation and in present pregnancy, the quadruple marker screen test had suggested a high risk for Trisomy 18 with the risk >1:50. The study was performed on the amniotic fluid and peripheral blood samples received at the clinical cytogenetics department. The interphase FISH and conventional karyotype methods were followed. The prenatal diagnosis using an amniotic fluid sample found a triploid fetus with unexpected balanced chromosomal translocation: 69, XXX,t(2;9)(q11.2;p22)x2. Later the origin of translocation was confirmed by parental chromosomal study. Cytogenetic analysis showed the presence of translocation involving chromosome 2 and 9 in the mother which confirms the maternal origin of translocation in fetal triploidy. Prenatal diagnosis of fetal triploidy with balanced translocation of maternal origin is a rare finding. In present study, the triploidy arises from the failure to expel the second polar body. It is important to perform prenatal fetal imaging with ultrasound at 18-22 weeks to identify any fetal anomalies or intrauterine growth retardation which is associated with triploidy.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"12 1","pages":"81-85"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71521402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Contribution of the Adrenergic, Cholinergic, and Serotonergic Systems to Leiomyoma Development and Treatment.","authors":"Ângel Inácio, Laura Aguiar, Raquel Carrilho, Patrícia Pires, Joana Ferreira, Luís Coelho, Mário Rui Mascarenhas, Luís Sardinha, Tiago Bilhim, João Pisco, Manuel Bicho, Maria Clara Bicho","doi":"10.22088/IJMCM.BUMS.12.4.320","DOIUrl":"10.22088/IJMCM.BUMS.12.4.320","url":null,"abstract":"<p><p>The link between the autonomic nervous system and tumor biology is being unfold. We aim to study the contribution of genes of the adrenergic (ADBR2 - rs1042713, NM_000024.6:c.46G>A, NP_000015.2:p. Gly16Arg), cholinergic (CHRNA5 - rs16969968, NM_000745.3:c.1192G>A, NP_000736.2:p.Asp398Asn), and serotonergic systems (SLC6A4 - 5-HTTVNTR-intron2, HTR2A - rs6313, NM_000621.5:c.102C>T, NP_ 001365853 .1: p. Ser 34=) to gynecological tumorigenesis and their treatment by embolization. A total of 517 DNA samples from women were analyzed. Samples were genotyped by PCR, PCR-RFLP and EndPoint genotyping. Results show a statistically significant association between the AA genotype of the ADBR2 gene and GG genotype of the CHRNA5 gene with leiomyoma (OR = 2.311; p = 0.003 and OR = 2.165; p = 0.001, respectively), and the epistatic interaction between genotypes increases the risk (OR = 2.458; p= 0.043). The GG genotype (CHRNA5) shows a lower reduction of the volume of the main leiomyoma after treatment (p=0.015). Combination of the genotypes 12/12-AA (SLC6A4 - ADBR2) increases the risk to leiomyoma (OR = 2.540, p= 0.030). TT genotype of HTR2A gene in combination with any of the two risk genotypes (of ADBR2 or CHRNA5) increases substantially the risk (OR = 5.266, p = 0.006; OR = 6.364, p=0.007, respectively). We conclude that ADBR2 and CHRNA5 genes have a relevant role that is enhanced by the epistatic relationship with the genes HTR2A and SLC6A4. CHRNA5 gene may also be a modulator of the success of embolization. We confirm the contribution of the genetics of Autonomous Nervous System to tumor biology.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"12 4","pages":"320-334"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11240054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stigmasterol- an Acetylcholinesterase Inhibitor from <i>Phormidium retzii</i> with relevance to Alzheimer's disease Therapy.","authors":"Rincy Yesudas, Vinoth Kumar Thirumalairaj, Geetharamani Durairaj, Amrutha Chacko, Lakshmanasenthil Shanmugaasokan, Suja Gunasekaran","doi":"10.22088/IJMCM.BUMS.12.2.100","DOIUrl":"10.22088/IJMCM.BUMS.12.2.100","url":null,"abstract":"<p><p>This study observed <i>in vitro</i> screening, purification and identification of cholinesterase inhibitors from the microalgae <i>Phormidium retzii</i>. Mixed microalgal culture was screened from freshwater samples for <i>Phormidium</i> sp. Single colony was purified and authenticated as <i>P. retzii</i>. Acetylcholinesterase (AChE) enzyme was purified from hRBC ghost. Sequential extraction of <i>P. retzii</i> was performed using organic solvents. Cholinesterase enzyme activity and its inhibition by various extracts were then tested. The active fractions were then subjected to partial purification and characterization. Petroleum ether extract of <i>P. retzii</i> showed maximum inhibition of 68.6 % against AChE while other solvent extracts showed no inhibition. Seven fractions were obtained from the active extract using thin layer chromatography. Among which fraction no. 5 showed maximum inhibition of 86.37 % towards AChE. Fraction no. 5 when subjected to GC-MS led to determination of the active principle as stigmasterol. The maximum inhibition of stigmasterol (0.45µM) was 81.2±0.08% with IC50 value of 0.214. Stigmasterol from <i>P. retzii</i> inhibited AChE projecting itself as safer drug for Alzheimer's disease with minimal side effects.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"12 2","pages":"100-107"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10837910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139681102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of OptrA Gene Mediated Linezolid Resistance among Enterococcus Faecium: A Pilot Study from a Tertiary Care Hospital, India.","authors":"Vandana Rani, Ajit Prakash, Mohammad Amin-Ul Mannan, Priyanka Das, Hitha Haridas, Rajni Gaindaa","doi":"10.22088/IJMCM.BUMS.12.3.242","DOIUrl":"10.22088/IJMCM.BUMS.12.3.242","url":null,"abstract":"<p><p><i>E. faecium</i> is the third most common cause of nosocomial infections. Linezolid (LNZ) is a reserve antibiotic recommended for infections caused by vancomycin resistant <i>E. faecium</i> (VREfm).  The aim of the present study was to investigate the prevalence of <i>optrA</i> gene among linezolid resistant <i>E. faecium</i> (LREfm) and to study the molecular epidemiology using pulse field gel electrophoresis (PFGE). Clinically significant LREfm were identified and antimicrobial susceptibility was performed by disc diffusion. Minimum inhibitory concentration (MIC) of linezolid, vancomycin, daptomycin and quinupristin/dalfopristin was determined by E-test. PCR and PCR-RFPL were performed for the detection of <i>optrA/cfr</i> gene and G2576T mutation respectively. Molecular epidemiology was studied by PFGE. A total of 1081 clinically significant <i>Enterococci</i> species were isolated which included <i>E. faecium</i> 63.5% (n=687) and <i>E. faecalis</i> 36.5% (n=394). LREfm (30/687) were further studied. Multidrug resistance and <b>v</b>ancomycin resistance was 100% and 80%, respectively. Linezolid MIC range was 8-256µg/ml and the most common mechanism of resistance was <i>optrA</i> gene (83.3%) followed by G2576T mutation (33.3%). PFGE analysis demonstrated 4 major clones. The <i>optrA</i> gene mediated linezolid resistance was high and PFGE suggests resistance was emerging in the different background strains irrespective of resistance mechanism. Studies are required to investigate factors driving the emergence of linezolid resistance. The review suggests that this is the first report of <i>optrA-</i>mediated resistance in <i>E. faecium</i> from India.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"12 3","pages":"242-256"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11092898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140945065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression Assessment of the <i>Helicobacter pylori</i><i>babA</i> and <i>sabA</i> Genes in Patients with Peptic Ulcer, Duodenal Ulcer and Gastric Cancer.","authors":"Javad Shokri Shirvani, Maryam Salehi, Amirmohammad Rezaei Majd, Farzin Sadeghi, Elaheh Ferdosi-Shahandashti, Soraya Khafri, Mehdi Rajabnia","doi":"10.22088/IJMCM.BUMS.12.2.211","DOIUrl":"10.22088/IJMCM.BUMS.12.2.211","url":null,"abstract":"<p><p><i>Helicobacter pylori</i> as a common gastrointestinal (GI) pathogen must possess certain virulence characteristics to colonize the stomach, evade host immune responses, and subsequently induce GI diseases. This research aimed to investigate the expression level of two important genes, the sialic acid-binding adherence (<i>SabA</i>) and the blood group antigen-binding adhesion (<i>BabA</i>) in <i>H. pylori</i> strains isolated from adult patients living in the northern part of Iran, and their association with peptic ulcer disease (PUD) and gastric cancer (GC). This cross-sectional study was carried out on adult patients referring to the GI clinic of the hospitals affiliated to Babol University of Medical Sciences, Iran. New cases diagnosed with gastritis, peptic ulcer or gastric cancer were included. Endoscopic-guided gastric biopsies were examined and <i>H. pylori</i> positive colonies were analyzed to determine the expression of <i>babA</i> and <i>sabA</i> genes, utilizing specific primers and the SYBR Green dye. Among 175 patients with mean age of 51.6±15.6 years, 101 (57.7%) of the individuals tested positive for H. pylori infection. Statistical analysis revealed a significant correlation between <i>sabA</i> (P=0.003) and <i>babA</i> (P=0.002) gene expression and development of PUD and GC. Smoking (P=0.052), gender (P=0.004) and positive <i>babA</i> gene expression (P=0.009) had the greatest association with occurrence of PUD or GC in <i>H. pylori</i> positive patients.  In summary, the presence of the <i>sabA</i> gene in people infected with <i>H. pylori</i> increased the risk of GC compared to gastritis, while, the presence of the <i>babA</i> gene was significantly increased in gastric ulcer patients. Considering the diversity of <i>H. pylori</i> isolates and the varying results observed in different geographical regions, further comprehensive studies are required to evaluate the function of these genes in <i>H. pylori</i> pathogenesis and their relationship with clinical outcomes.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"12 2","pages":"211-219"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10837913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139681098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Fluorapatite/Bioactive Glass Nanocomposite Foams as Bone Tissue Scaffold: An in Vivo Study.","authors":"Seyedali Seyedmajidi, Maryam Seyedmajidi, Sina Haghanifar","doi":"10.22088/IJMCM.BUMS.12.4.388","DOIUrl":"10.22088/IJMCM.BUMS.12.4.388","url":null,"abstract":"<p><p>The present study investigated the suitability of nanocomposite foams of fluorapatite and bioactive glass (FA /BG) in different weight ratios as scaffolds for bone tissue in rat tibia regeneration to determine the optimal composition. FA and BG nano powders with a weight ratio of 25% FA/75% BG (compound 1) and 75% FA/25% BG (compound 2) were used as precursors for gel casting to produce nanocomposite foams. Thirty rats were randomly divided into two equal groups. Disk-shaped samples of each compound were implanted into the tibias of 15 rats. After 15, 30, or 60 days, five rats from each group were sacrificed and subjected to radiological, histopathological, and histomorphometrical examination. Data were analyzed using SPSS software. No foreign body reaction was observed in either group at all intervals, and the bone-biomaterial junction was direct. Overall, the inflammation rate, and the number of blood vessels, osteoblasts, and osteoclasts decreased over time in both groups. However, the number of osteocytes, trabecular bone thickness, and the percentage of new bone formation increased, in contrast to the remaining biomaterial percentage. Most of the changes in the group implanted with compound 2 were significantly more significant and faster than in the other group. Although the composite with the higher percentage of FA was superior to the composite with the higher percentage of BG, considering the results of our previous similar studies, the composite with the same percentage of FA and BG is more favorable to be used as a substitute for bone tissue in the body.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"12 4","pages":"388-400"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11240056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amyloid Beta Alters the Expression of microRNAs Regulating HMGCR and ABCA1 Genes in Astrocytes of C57BL/6J Mice.","authors":"Hossein Azizi Dariuni, Mehrnaz Karimi Darabi, Zahra Nazeri, Shirin Azizidoost, Alireza Kheiroallah, Azam Khedri, Maryam Cheraghzadeh","doi":"10.22088/IJMCM.BUMS.12.1.30","DOIUrl":"10.22088/IJMCM.BUMS.12.1.30","url":null,"abstract":"<p><p>Dysregulation of brain cholesterol homeostasis causes the accumulation of extracellular protein deposits called amyloid plaques in the hippocampus which eventually leads to neuronal death, memory and learning deficits. The aim of the present study was to investigate the effect of beta amyloid on miRNAs regulating HMGCR and ABCA1 as cholesterol synthesis and homeostasis genes. Primary astrocytes were isolated from C57BL/6J mice, and were treated with 0.5 μM amyloid beta (Aβ). Expression levels of genes and miRNAs were measured by real-time PCR. In comparison to control, Aβ treatment resulted in a significant decrease in miR-96-5p expression as a positive and negative regulator of HMGCR and ABCA1, respectively. There was no significant increase in miR-27a-3p expression as a negative regulator of HMGCR. miR- 106b- 5p and miR-143-3p expressions were also dramatically decreased as ABCA1 negative regulators. Amyloid beta can alter the expression of major genes in the cholesterol homeostasis pathway via their regulatory miRNAs.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"12 1","pages":"30-39"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71521381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory Responses of Women with Polycystic Ovary Syndrome <i>in Vitro</i> Differ from Healthy Women.","authors":"Fatemeh Rezayat, Mehri Hajiaghayi, Nazanin Ghasemi, Mehrnaz Mesdaghi, Fahimeh Ramezani Tehrani, Nariman Mosaffa","doi":"10.22088/IJMCM.BUMS.12.1.70","DOIUrl":"10.22088/IJMCM.BUMS.12.1.70","url":null,"abstract":"<p><p>Polycystic ovary syndrome is a low-grade inflammatory state with increased serum levels of TNF-α. The present study has compared the inflammatory responses to breast cancer cell lines in women with PCOS with healthy women. Peripheral blood mononuclear cells (PBMCs) isolated from 50 women with PCOS and 50 healthy controls were cultured in the trans-well co-culture system. These cells were stimulated with two distinct breast cancer cell lines. The proliferation of PBMCs, CD3+CD8+T cell percentages, and tumor necrosis factor-alpha (TNF-α) concentration were evaluated after 48 and 72 hours of incubation. TNF-α concentration and the proliferation rate of PBMCs after 48 hours of incubation significantly increased in the PCOS group. However, after 72 hours, TNF-α secretion significantly decreased in the PCOS group. The ability of PBMCs to produce TNF-α decreased gradually in women with PCOS. When the effects of low-grade inflammation and endocrine conditions on the cells decrease, the inability of PBMCs to create an inflammatory response will be altered.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"12 1","pages":"70-80"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71521383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal Stem Cells Therapy Led to the Improvement of Spatial Memory in Rats with Alzheimer's disease Through Changing the Expression of LncRNA TUSC7/ miR-449a/ PPARγ and CD36 Genes in the Brain Tissue.","authors":"Seyedeh Pardis Pezeshki, Mehrnaz Karimi Darabi, Zahra Nazeri, Alireza Sarkaki, Mojtaba Rashidi, Hossein Babaahmadi-Rezaei, Alireza Kheirollah, Maryam Cheraghzadeh","doi":"10.22088/IJMCM.BUMS.12.2.108","DOIUrl":"10.22088/IJMCM.BUMS.12.2.108","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs) have the ability to phagocytize amyloid beta (Aβ) plaques and lower inflammation through the activity of microglia. Peroxisome proliferator-activated receptor gamma (PPARγ) is a protein involved in reducing inflammation through the activity of microglia and the phagocytosis of Aβ plaques by scavenger receptor CD36, in this study, the effect of MSCs therapy on memory function and plaques was investigated. A total of 24 adult male Wistar rats were randomly divided into three groups:1) the control group, 2) the Aβ-treated group (Alzheimer's disease (AD)), and 3) the MSC-treated group (AD + MSC). After the treatment with Aβ and MSCs, western blotting and real-time polymerase chain reaction (PCR) techniques were used to assess protein and gene expression levels, respectively. MSCs improved spatial learning and memory in the AD group (p ≤0.05). The expression levels of PPARγ, lncRNA TUSC7, and CD36 genes were significantly elevated in the group receiving MSCs compared to the AD group (p≤0.0001). Also, the expression level of miR-449a significantly decreased in the AD + MSC group (p≤0.0001). Moreover, western blot analysis revealed that PPARγ and CD36 protein levels were enhanced in the AD + MSC group compared to the AD group (p≤0.0001). MSC treatment led to the positive regulation of the PPARγ gene and its protein expression by ncRNAs, which could have a beneficial impact on CD36 protein levels, and subsequently, reduce the number of plaques in the cell recipient.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"12 2","pages":"108-119"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10837912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139681101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Capparis spinosa</i> Fruit Hydroalcoholic Extract on Paraquat-Induced Pulmonary Fibrosis in the Rat.","authors":"Tahmine Ostovar, Sahar Rezaei, Hajar Shokri-Afra, Sadra Samavarchi Tehrani, Fatemeh Namvarjah, Masoume Aliabadi, Hosein Effatpanah, Hemen Moradi-Sardareh","doi":"10.22088/IJMCM.BUMS.12.4.423","DOIUrl":"10.22088/IJMCM.BUMS.12.4.423","url":null,"abstract":"<p><p>Pulmonary fibrosis (PF) is a lethal inflammatory disease and there has been no effective medication for this progressive disease up to now. Paraquat is commonly used in agricultural settings to control weed growth and is one of the important risk factors for PF. Additionally, emerging evidence has demonstrated <i>Capparis spinosa</i> (<i>C. spinose</i>) fruit extract has anti-fibrotic, anti-inflammatory, and antioxidant properties. We aimed to evaluate whether <i>C. spinose</i> fruit hydroalcoholic extract has a positive effect against Paraquat-induced PF in rats. 30 male Wistar rats were randomly divided into 5 groups, which included: a control group, a Paraquat control group, a <i>C. spinose</i> group with a dose of 20 mg/kg, a <i>C. spinose</i> group with a dose of 30 mg/kg, a <i>C. spinose</i> group with a dose of 50 mg/kg. After 21 days of the treatment, levels of hydroxyproline and malondialdehyde (MDA) in lung tissue were assessed and lung indices and semi-quantitative histopathological changes were determined. The results showed that treatment with <i>C. spinose</i>, led to increased weight gain, whereas reduced lung weight. <i>C. spinose</i> demonstrated a decreasing effect on levels of MDA, and hydroxyproline in lung tissue. Moreover, histopathological data and the number of lung indices indicated the preventive role of <i>C. spinose</i> Paraquat-induced PF in rats.</p>","PeriodicalId":14152,"journal":{"name":"International Journal of Molecular and Cellular Medicine","volume":"12 4","pages":"423-434"},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11240055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}