International Journal of Laboratory Hematology最新文献

{"title":"Verification of an Algorithm for Detection of Unstable Haemoglobin Variants With Sysmex XN-10 Yielded a High Degree of False Positives","authors":"Madeleen Bosma, Richard M. Noordervliet, Mercedeh Tajdar, Christine van Laer","doi":"10.1111/ijlh.14467","DOIUrl":"10.1111/ijlh.14467","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 4","pages":"748-749"},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CD200 Expression by CD4+ T Cells Using Flow Cytometry as a Valuable Marker for Distinguishing Hodgkin Lymphoma From Inflammatory Conditions","authors":"Valentin Pourchet, Radu Chiriac, Lucile Baseggio","doi":"10.1111/ijlh.14471","DOIUrl":"10.1111/ijlh.14471","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 4","pages":"750-755"},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Expression of SPAG6 Acts as a Pro-Tumor Factor and Associated With Poor Prognosis in Acute Myeloid Leukemia","authors":"Jie Luo,&nbsp;Haiqiu Zhao,&nbsp;Xiaoyan Zang,&nbsp;Lin Liu","doi":"10.1111/ijlh.14457","DOIUrl":"10.1111/ijlh.14457","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Acute myeloid leukemia (AML) is a malignant hematological disease that has shown an increased prevalence in recent years. Despite advancements in treatment, significant limitations remain. Therefore, more information about AML mechanisms is essential to improve therapeutic strategies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>RT-qPCR, IHC, and western blot were used to analyze SPAG6 expression levels. The association between SPAG6 expression and patient survival was evaluated using LeukemiaDB, GEPIA, and Bloodspot databases. Cell viability was detected by CCK-8 assay. Flow cytometry was applied to measure cell apoptosis and cell cycle.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Database analysis revealed elevated SPAG6 expression in AML. Subsequent RT-qPCR confirmed high SPAG6 expression in AML, MDS, MPN, and ALL samples. Clinical data analysis demonstrated a positive correlation between SPAG6 expression and risk stratification in AML patients. Notably, it was found that the overall survival time of AML patients with high SPAG6 expression was shorter than that of patients with low SPAG6 expression. Moreover, SPAG6 knockdown in the AML cell line HL60 promoted apoptosis and arrested the cell cycle in the G1 phase.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Therefore, we believe that SPAG6 may be a pro-tumor factor in AML.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 4","pages":"680-689"},"PeriodicalIF":2.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplement Article","authors":"","doi":"10.1111/ijlh.14436","DOIUrl":"https://doi.org/10.1111/ijlh.14436","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 S1","pages":"3-34"},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of the Performance of Automated Digital Cell Morphology Analyzers for Leukocyte Differentiation in Hematologic Malignancies: Mindray MC-80 Versus West Medical Vision Hema","authors":"Sara Sacchetti,&nbsp;Matteo Bellia,&nbsp;Matteo Vidali,&nbsp;Valentina Zanotti,&nbsp;Luca Giacomini,&nbsp;Gianluca Gaidano,&nbsp;Andrea Patriarca,&nbsp;Umberto Dianzani,&nbsp;Roberta Rolla","doi":"10.1111/ijlh.14470","DOIUrl":"10.1111/ijlh.14470","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>The use of artificial intelligence in hematology laboratories has improved the diagnostic evaluation of peripheral blood cells. The aim of this study is to compare the performance of two automated digital cell morphology analyzers, the Mindray MC-80 and the West Medical Vision Hema Pro, with manual microscopy, the gold standard, for leukocyte differentiation in patients with hematologic malignancies and infections.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Peripheral blood smears from 75 patients were analyzed, including cases of acute lymphoblastic leukemia (ALL, 4), chronic lymphocytic leukemia (CLL, 20), acute myeloid leukemia (AML, 20), chronic myeloid leukemia (CML, 5), other lymphoproliferative disorders (LPD, 20), and infections (6). The agreement between microscopy, Vision Hema, and MC-80 was assessed by Bland–Altman analysis for eight leukocyte populations (neutrophils, lymphocytes, monocytes, eosinophils, basophils, band cells, myelocytes, and metamyelocytes).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Vision Hema demonstrated better agreement with manual microscopy for eight normally expected leukocyte populations (neutrophils, lymphocytes, monocytes, eosinophils, basophils, band cells, myelocytes, and metamyelocytes), whereas MC-80 exhibited greater biases, particularly in lymphocytes, basophils, and immature granulocytes. For pathologic cells, VH significantly overestimated blasts, while MC-80 classified them more accurately, showing better agreement with manual microscopy in acute leukemias. Additionally, MC-80 showed potential clinical value in detecting abnormal lymphocytes and promyelocytes, which may be relevant for hematologic malignancies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Vision Hema provides more reliable classification of normally expected leukocyte populations, while MC-80 shows advantages in detecting abnormal cells, particularly in hematologic malignancies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 4","pages":"643-650"},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient Moving Average for Continuous Real-Time QC; Real-World Application Illustrated","authors":"Huub H. van Rossum,&nbsp;Jean-Marc Giannoli,&nbsp;Tony Badrick","doi":"10.1111/ijlh.14462","DOIUrl":"10.1111/ijlh.14462","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"570-573"},"PeriodicalIF":2.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sysmex XN-Based Evaluation of the Diagnostic Performance of High-Fluorescent Cells From CSF as a Supportive Diagnostic Criterion in Neurological Diseases","authors":"Benedict Schwarz,&nbsp;Christopher Hardt,&nbsp;Katharina Friedrich,&nbsp;Monika Prpic,&nbsp;Anja Osterloh,&nbsp;Frank L. Heppner,&nbsp;Klemens Ruprecht,&nbsp;Kai Kappert,&nbsp;Amir Jahic","doi":"10.1111/ijlh.14466","DOIUrl":"10.1111/ijlh.14466","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Automatic cytological analysis of cerebrospinal fluid (CSF) by Sysmex XN-Series represents a convenient laboratory platform for quantitative examination of nucleated CSF cells (monomorphonuclear (MN), polymorphonuclear (PMN), high-fluorescent (HF)). HF cells (HFC), a research laboratory parameter so far, seem to be associated with certain clinical patterns. Hence, we aimed to determine the diagnostic HFC value for different clinical categories in neurological settings.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Morphological classification of automatically detected HFC was carried out using manual light microscopy. Automatic method precision for cell differentiation was evaluated in comparison. In 284 cases, multiple correlation strategies and mathematical disease modellings enabled an explorative analysis of HFC suitability for case stratification into the categories Hemorrhage, Inflammation, Neoplasia, other, and unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Manual microscopic reevaluation revealed plasma cells, macrophages, and malignant cells being HFC correlates in 80% of automatically detected HFC. Method correlation for automatic and manual CSF cell differentiation approaches was 95%, yielding a negative bias of 4.3% for MN and positive bias of 0.4% and 3.9% for PMN and HF, respectively. When HFC were used as a “stand-alone” predicting tool, diagnostic accuracy, specificity, and sensitivity depended on the clinical condition, ranging from &gt; 0.5 up to &gt; 0.7 for Hemorrhage, Inflammation, and Neoplasia. However, multiparametric correlation analyses combining laboratory CSF diagnostics and mathematical methods defined HFC as a relevant laboratory parameter for adequate clinical case stratification. With an expected random distribution of 25% for four clinical categories, almost 70% of cases were correctly classified when the HFC-based mathematical algorithm was applied.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>HFC has significant diagnostic and/or predictive value for neurological diseases.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 4","pages":"632-642"},"PeriodicalIF":2.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14466","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mast Cell Blast Crisis in a Patient of Chronic Myeloid Leukemia With Concurrent BCR::ABL1 and RUNX1::RUNX1T1 Rearrangement or Should We Call It Myelomastocytic Leukemia? A Diagnostic Challenge With Nomenclature Dilemma","authors":"Aastha Gupta,&nbsp;Karthika Rudrakumar,&nbsp;Surbhi Singh,&nbsp;Shrinidhy Nathany,&nbsp;Rahul Bhargava","doi":"10.1111/ijlh.14465","DOIUrl":"10.1111/ijlh.14465","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 5","pages":"783-785"},"PeriodicalIF":2.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Germline Variants in Idiopathic Erythrocytosis Identified by Multigene Panel Sequencing","authors":"Min-Seung Park,&nbsp;Boram Kim,&nbsp;Hyun-Young Kim,&nbsp;Chul Won Jung,&nbsp;Jun Ho Jang,&nbsp;Hee-Jin Kim","doi":"10.1111/ijlh.14458","DOIUrl":"10.1111/ijlh.14458","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 3","pages":"563-566"},"PeriodicalIF":2.2,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards Sensitive and Cost-Effective Chimerism Assays Using FABCASE: A Fast Approach for Estimating Assay Informativity","authors":"Matthijs Vynck","doi":"10.1111/ijlh.14460","DOIUrl":"10.1111/ijlh.14460","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Chimerism monitoring is part of the standard of care for patients following an allogeneic hematopoietic stem cell transplantation. There has recently been a move towards sensitive, high-throughput (next-generation) sequencing analysis of biallelic markers for this purpose. Determining the number and properties of the markers to include in an assay to achieve reliable yet cost-effective chimerism quantification is an underexposed but critical part of chimerism assay development, optimization, and validation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We develop <i>FABCASE</i> (Fast and Accurate Biallelic Chimerism Assay Size Estimation), an approach to estimate the required number of markers to screen to obtain a given informativity rate. We explore several practical examples that illustrate the diverse applications of <i>FABCASE</i> beyond calculating the required number of markers to screen.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p><i>FABCASE</i> offers a more than four orders of magnitude speed improvement compared to a previously introduced Monte Carlo simulation approach, unlocking extensive <i>in silico</i> scenario analyses. We find that minor allele frequency (MAF) and informative rate estimation based on small sample series (tens) are reasonably accurate. MAFs may vary drastically between populations, and the number of required markers to attain a preset informativity rate is inflated (here, +28%) when not optimized. Marker subset selection from a pool of candidate markers is little impacted by small-to-medium MAF differences (0%–20% MAF). Prioritizing markers with uniform amplification efficiency reduces sequencing depth requirements and improves cost-effectiveness. A web graphical user interface facilitating marker set informativity evaluation is available at https://mvynck.shinyapps.io/FABCASE.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>FABCASE facilitates the design, refinement, and implementation of sensitive and cost-effective chimerism assays. Due attention should be given to an assay's marker MAFs, sensitivities, and amplification efficiencies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 4","pages":"690-697"},"PeriodicalIF":2.2,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}