{"title":"高表达的SPAG6在急性髓系白血病中作为促肿瘤因子并与不良预后相关。","authors":"Jie Luo, Haiqiu Zhao, Xiaoyan Zang, Lin Liu","doi":"10.1111/ijlh.14457","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Acute myeloid leukemia (AML) is a malignant hematological disease that has shown an increased prevalence in recent years. Despite advancements in treatment, significant limitations remain. Therefore, more information about AML mechanisms is essential to improve therapeutic strategies.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>RT-qPCR, IHC, and western blot were used to analyze SPAG6 expression levels. The association between SPAG6 expression and patient survival was evaluated using LeukemiaDB, GEPIA, and Bloodspot databases. Cell viability was detected by CCK-8 assay. Flow cytometry was applied to measure cell apoptosis and cell cycle.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Database analysis revealed elevated SPAG6 expression in AML. Subsequent RT-qPCR confirmed high SPAG6 expression in AML, MDS, MPN, and ALL samples. Clinical data analysis demonstrated a positive correlation between SPAG6 expression and risk stratification in AML patients. Notably, it was found that the overall survival time of AML patients with high SPAG6 expression was shorter than that of patients with low SPAG6 expression. Moreover, SPAG6 knockdown in the AML cell line HL60 promoted apoptosis and arrested the cell cycle in the G1 phase.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Therefore, we believe that SPAG6 may be a pro-tumor factor in AML.</p>\n </section>\n </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 4","pages":"680-689"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"High Expression of SPAG6 Acts as a Pro-Tumor Factor and Associated With Poor Prognosis in Acute Myeloid Leukemia\",\"authors\":\"Jie Luo, Haiqiu Zhao, Xiaoyan Zang, Lin Liu\",\"doi\":\"10.1111/ijlh.14457\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Acute myeloid leukemia (AML) is a malignant hematological disease that has shown an increased prevalence in recent years. Despite advancements in treatment, significant limitations remain. Therefore, more information about AML mechanisms is essential to improve therapeutic strategies.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>RT-qPCR, IHC, and western blot were used to analyze SPAG6 expression levels. The association between SPAG6 expression and patient survival was evaluated using LeukemiaDB, GEPIA, and Bloodspot databases. Cell viability was detected by CCK-8 assay. Flow cytometry was applied to measure cell apoptosis and cell cycle.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Database analysis revealed elevated SPAG6 expression in AML. Subsequent RT-qPCR confirmed high SPAG6 expression in AML, MDS, MPN, and ALL samples. Clinical data analysis demonstrated a positive correlation between SPAG6 expression and risk stratification in AML patients. Notably, it was found that the overall survival time of AML patients with high SPAG6 expression was shorter than that of patients with low SPAG6 expression. Moreover, SPAG6 knockdown in the AML cell line HL60 promoted apoptosis and arrested the cell cycle in the G1 phase.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>Therefore, we believe that SPAG6 may be a pro-tumor factor in AML.</p>\\n </section>\\n </div>\",\"PeriodicalId\":14120,\"journal\":{\"name\":\"International Journal of Laboratory Hematology\",\"volume\":\"47 4\",\"pages\":\"680-689\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-03-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Laboratory Hematology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/ijlh.14457\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Laboratory Hematology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ijlh.14457","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}
High Expression of SPAG6 Acts as a Pro-Tumor Factor and Associated With Poor Prognosis in Acute Myeloid Leukemia
Background
Acute myeloid leukemia (AML) is a malignant hematological disease that has shown an increased prevalence in recent years. Despite advancements in treatment, significant limitations remain. Therefore, more information about AML mechanisms is essential to improve therapeutic strategies.
Methods
RT-qPCR, IHC, and western blot were used to analyze SPAG6 expression levels. The association between SPAG6 expression and patient survival was evaluated using LeukemiaDB, GEPIA, and Bloodspot databases. Cell viability was detected by CCK-8 assay. Flow cytometry was applied to measure cell apoptosis and cell cycle.
Results
Database analysis revealed elevated SPAG6 expression in AML. Subsequent RT-qPCR confirmed high SPAG6 expression in AML, MDS, MPN, and ALL samples. Clinical data analysis demonstrated a positive correlation between SPAG6 expression and risk stratification in AML patients. Notably, it was found that the overall survival time of AML patients with high SPAG6 expression was shorter than that of patients with low SPAG6 expression. Moreover, SPAG6 knockdown in the AML cell line HL60 promoted apoptosis and arrested the cell cycle in the G1 phase.
Conclusion
Therefore, we believe that SPAG6 may be a pro-tumor factor in AML.
期刊介绍：
The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology.
The journal publishes invited reviews, full length original articles, and correspondence.
The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines.
The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.
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