Aline Rosignoli da Conceição, Leidjaira Lopes Juvanhol, Aline Marcadenti, Ângela Cristine Bersch-Ferreira, Rachel H Vieira Machado, Josefina Bressan
{"title":"Improving cardiometabolic risk by dietary quality changes in secondary care patients in cardiology: four-year follow-up from BALANCE Program Trial.","authors":"Aline Rosignoli da Conceição, Leidjaira Lopes Juvanhol, Aline Marcadenti, Ângela Cristine Bersch-Ferreira, Rachel H Vieira Machado, Josefina Bressan","doi":"10.1080/09637486.2026.2675677","DOIUrl":"10.1080/09637486.2026.2675677","url":null,"abstract":"<p><p>We aimed to assess whether changes in the Brazilian Cardioprotective Nutritional Program Trial diet quality through the Dietary Inflammatory Index (DII), dietary Total Antioxidant Capacity (dTAC), overall, healthy and unhealthy Plant-Based Diet Index (PDI, hPDI and uPDI, respectively), and modified Alternative Healthy Eating Index (mAHEI) contributed to the improvement of cardiometabolic risk markers in patients with established CVD during 48 months of follow-up. The delta (changes) of DII and dTAC were positively and negatively associated, respectively, with the body mass index and waist-to-height ratio (WHtR) deltas. Also, the PDI and hPDI deltas were negatively associated with total cholesterol, low-density lipoprotein cholesterol, and fasting glucose deltas. Finally, the uPDI delta was positively associated with fasting glucose and systolic blood pressure deltas and the mAHEI delta was also negatively associated with the WHtR and triglycerides deltas. Thus, adherence to a healthier dietary pattern may impact positively cardiometabolic risk factors in this sample.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"384-396"},"PeriodicalIF":3.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147981743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Danila Cianciosi, Yasmany Armas Diaz, Bei Yang, Zexiu Qi, Ge Chen, José L Quiles, Massimiliano Gasparrini, Manuela Cassotta, Rubén Calderón Iglesias, Maurizio Battino, Francesca Giampieri
{"title":"Liquorice alters adipocyte-breast cancer cell crosstalk by modulating oxidative stress and suppressing aromatase and renin-angiotensin signalling.","authors":"Danila Cianciosi, Yasmany Armas Diaz, Bei Yang, Zexiu Qi, Ge Chen, José L Quiles, Massimiliano Gasparrini, Manuela Cassotta, Rubén Calderón Iglesias, Maurizio Battino, Francesca Giampieri","doi":"10.1080/09637486.2026.2666404","DOIUrl":"10.1080/09637486.2026.2666404","url":null,"abstract":"<p><p>Obesity is recognised to be a risk factor for breast cancer since adipose tissue influences the tumour microenvironment. This study aims to investigate the effect of the secretome of 3T3-L1 adipocytes untreated or treated with liquorice root extract (LRE), containing flavonoids, phenolic acids, and saponins on MCF-7 breast cancer cells. By treating adipocytes with LRE, the secretion of certain pro-tumorigenic factors like IGFBP-6, resistin, and VEGF was reduced. MCF-7 cells exposed to conditioned medium from LRE-treated adipocytes exhibited an increase in reactive oxygen species levels, downregulation of the Nrf2 antioxidant pathway, and increased autophagy. Those conditions reduced cell viability, migration, and colony formation. Additionally, there was downregulation of genes associated with oestrogen signalling and tumour-related processes, including CYP19A1 (aromatase), ERα, Her2, and components of the renin-angiotensin system (RAS). These findings suggest that LRE can modulate the adipocyte secretome to influence breast cancer cell behaviour under obesity-related <i>in vitro</i> conditions.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"326-349"},"PeriodicalIF":3.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147929021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Validation of an Italian version of a questionnaire to assess home cooking skills: HCSQ-ITA.","authors":"Federica Quintiero, Daniele Nucci, Aline Rissatto Teixeira, Betzabeth Slater Villar, Lenycia de Cassya Lopes Neri, Cinzia Ferraris","doi":"10.1080/09637486.2026.2670766","DOIUrl":"10.1080/09637486.2026.2670766","url":null,"abstract":"<p><p>Cooking skills significantly influence diet quality and help prevent chronic diseases. However, no validated instrument is currently available to assess home-cooking skills in Italy. This study aimed to culturally adapt and validate an Italian version of the Brazilian Home-Cooking Skills Assessment Scale for both nutrition professionals and the general population in Italy. The study involved forward-backward translation, Delphi technique validation with eight experts, pilot testing with 30 participants, and psychometric validation with 357 individuals (49.9% nutrition professionals). The Content Validity Index was above 0.9. Exploratory factor analysis revealed a three-factor structure explaining 50.9% of the variance, with strong model-fit indices. Reliability assessments showed excellent internal consistency (Cronbach's 𝛼 = 0.885) and high test-retest reliability (ICC = 0.88). The final instrument comprised 24 items distributed across four skill levels. The Home Cooking Skills Questionnaire - Italian version is a validated tool for assessing home-cooking skills in Italy and demonstrates strong psychometric properties.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"363-371"},"PeriodicalIF":3.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147948285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alberto Dolci, Alessandro Scuderi, Evelyn Frias-Toral, Leonardo Hernández, Andrea Di Mauro, Fabrizio Furnari, Alice Rosi, Francesca Scazzina, Giuseppe Grosso
{"title":"Environmental burden of fish in healthy and sustainable diets.","authors":"Alberto Dolci, Alessandro Scuderi, Evelyn Frias-Toral, Leonardo Hernández, Andrea Di Mauro, Fabrizio Furnari, Alice Rosi, Francesca Scazzina, Giuseppe Grosso","doi":"10.1080/09637486.2026.2658821","DOIUrl":"10.1080/09637486.2026.2658821","url":null,"abstract":"<p><p>Fish is widely promoted as part of healthy dietary patterns. The aim of this review was to summarise current literature on the environmental footprint of fish and its role within sustainable diets. Fish generally represents a minor share of total dietary environmental impacts, contributing to a smaller proportion of greenhouse-gas emissions (GHGe), land and water use than meat and other animal products. Several modelling studies showed that substituting meat with fish or increasing fish intake within optimised dietary patterns can reduce environmental impacts, although the magnitude varies by country, diet type, and fish species. However, some analyses reported increased GHGe associated with higher fish intake, especially in models ensuring nutritional quality. Overall, fish consumption is compatible with achieving nutritionally adequate and lower environmental impacts, although optimal match between environmental boundaries and nutritional needs is not always possible. These findings suggest that fish can play a constructive role in sustainable diets when integrated thoughtfully within broader dietary shifts.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"289-313"},"PeriodicalIF":3.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Association of circulating branched-chain or aromatic amino acids based on metabolome and apolipoprotein E genotype with incident dementia: a large cohort study.","authors":"Ziwei Liu, Yongjun Ai, Yue Wu, Yuncheng Zhang, Jianian Hua, Tong Liu, Dong Liu, Guo-Chong Chen, Guangfei Xu, Li-Hua Chen","doi":"10.1080/09637486.2026.2649766","DOIUrl":"10.1080/09637486.2026.2649766","url":null,"abstract":"<p><p>The associations between branched-chain amino acids (BCAAs) and dementia in individuals with chronic diseases remain unclear, and evidence on aromatic amino acids (AAAs) is limited. Using metabolic biomarker data from 117,892 UK Biobank participants, we found that higher circulating levels of BCAAs (HR=0.56, 95% CI: 0.41-0.76) and AAAs (HR=0.74, 95% CI: 0.55-0.98) were associated with lower risks of all-cause dementia and Alzheimer's disease, but not vascular dementia. Stratified analyses showed stronger inverse associations for BCAAs among women and individuals without diabetes (both <i>P</i> for interaction = 0.001). Similarly, AAAs were inversely associated with dementia risk primarily in non-diabetic participants, but not modified by hypertension or dyslipidemia. These findings highlight the potential protective roles of BCAAs and AAAs and underscore the importance of sex and diabetes status in dementia prevention.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"248-258"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Tristan Asensi, M Marinoni, L Costantini, P Degli Innocenti, G Pagliai, S Lotti, M Rendine, E Bertolotti, C Barbero Mazzucca, F Bibi, D Giustozzi, A Napoletano, P Biscotti, G Vici, M Guglielmetti, G Di Costanzo, V Gianfredi, C Marzi, C Del Bo', B Colombini, F Sofi, A Rosi, M Dinu
{"title":"The impact of perceived stress on adherence to the Mediterranean diet, eating behaviour and quality of life in a group of Italian adolescents.","authors":"M Tristan Asensi, M Marinoni, L Costantini, P Degli Innocenti, G Pagliai, S Lotti, M Rendine, E Bertolotti, C Barbero Mazzucca, F Bibi, D Giustozzi, A Napoletano, P Biscotti, G Vici, M Guglielmetti, G Di Costanzo, V Gianfredi, C Marzi, C Del Bo', B Colombini, F Sofi, A Rosi, M Dinu","doi":"10.1080/09637486.2026.2657945","DOIUrl":"10.1080/09637486.2026.2657945","url":null,"abstract":"<p><p>This cross-sectional study assessed perceived stress levels in a group of Italian adolescents and examined potential relationships with diet quality, eating behaviours and health-related quality of life (HR-QoL). A total of 1627 adolescents (52% male, median age 16 years) completed a survey including the Perceived Stress Scale (PSS-10), the KIDMED for Mediterranean diet (MD) adherence, the KIDSCREEN-10 for HR-QoL, and the Dutch Eating Behaviour Questionnaire for Children. Only 16.5% reported low stress levels, while 21.8% exhibited high perceived stress. Perceived stress was inversely associated with MD adherence (<i>β</i> = -0.06; <i>p</i> < 0.0001) and HR-QoL (<i>β</i> = -0.45; <i>p</i> < 0.0001), and positively associated with dysregulated eating behaviours, including emotional eating (<i>β</i> = 0.02; <i>p</i> value < 0.0001), external eating (<i>β</i> = 0.01; <i>p</i> < 0.0001) and restrained eating (<i>β</i> = 0.01; <i>p</i> < 0.0001). These results reinforce the importance of adolescent mental health and the need for strategies aimed at reducing stress and promoting balanced dietary patterns as MD.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"279-287"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147698583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huaqi Zhang, Qian Liang, Xi Chen, Quan Gan, Tianqi Tan, Weiming Wang, Jin Liu, Chuang Yang, Chunrong Zhong, Shangzhi Xu, Xiyu Cao, Meng Wu, Guoqiang Sun, Guoping Xiong, Hongying Yang, Xuefeng Yang, Liping Hao, Nianhong Yang
{"title":"Association between maternal yogurt consumption during late pregnancy and risk of infantile diarrhoea: a prospective cohort study.","authors":"Huaqi Zhang, Qian Liang, Xi Chen, Quan Gan, Tianqi Tan, Weiming Wang, Jin Liu, Chuang Yang, Chunrong Zhong, Shangzhi Xu, Xiyu Cao, Meng Wu, Guoqiang Sun, Guoping Xiong, Hongying Yang, Xuefeng Yang, Liping Hao, Nianhong Yang","doi":"10.1080/09637486.2026.2654462","DOIUrl":"10.1080/09637486.2026.2654462","url":null,"abstract":"<p><p>Yogurt is commonly recommended during pregnancy, but its association with infantile diarrhoea remains unclear. This prospective study was embedded in the Tongji Maternal and Child Health Cohort. Yogurt intake was assessed using a food frequency questionnaire and categorised based on frequency, amount and their combination. Infantile diarrhoea was diagnosed by doctors. Poisson regression models were used to estimate the adjusted relative risks (aRRs). Among 2161 mother-infant pairs, 72.9% mothers consumed yogurt, and 24.2% infants experienced diarrhoea. Each 100 g/week increase or each 1 time/week increase in yogurt intake were associated with reduced risks of infantile diarrhoea, with aRRs (95% CI) of 0.98 (0.96, 0.99) and 0.96 (0.94, 0.99), respectively. Compared with non-consumers, regular high consumers (≥3 times/week and ≥600 g/week had a significantly lower risk of infantile diarrhoea (aRR: 0.79, 95% CI: 0.65, 0.96). Regular high yogurt intake during late pregnancy may help protect infants from diarrhoea.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"271-278"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147673052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Trent Bozanich, Jack Dalla Via, Simone Radavelli-Bagatini, Cassandra Smith, Elsa Dent, Anthony Villani, Robin M Daly, Michael Tieland, Jonathan M Hodgson, Kun Zhu, Richard L Prince, Marc Sim
{"title":"Association between protein intake from animal and plant sources and the presence of frailty in community-dwelling Australian women.","authors":"Trent Bozanich, Jack Dalla Via, Simone Radavelli-Bagatini, Cassandra Smith, Elsa Dent, Anthony Villani, Robin M Daly, Michael Tieland, Jonathan M Hodgson, Kun Zhu, Richard L Prince, Marc Sim","doi":"10.1080/09637486.2026.2644869","DOIUrl":"10.1080/09637486.2026.2644869","url":null,"abstract":"<p><p>We investigated the cross-sectional association between total, animal and plant protein intake with the presence of a multidimensional measure of frailty in older women. Participants included 1380 community-dwelling Australian women, mean (SD) age 75.1 (2.7) years. A validated food frequency questionnaire was used to determine total, plant, and animal protein intake. Frailty was assessed using a frailty index across numerous health domains (scored 0-1, ≥0.25 indicating frailty). Cross-sectional associations were analysed using multivariable-adjusted logistic regression models. In total, 275 (19.9%) women were identified as frail. A non-linear inverse association was observed between higher intakes of total protein and the odds for frailty. Compared to lowest protein intakes (Quartile [Q]1, 0.77 g/kg BW/d), greater intakes (up to 1.66 g/kg BW/d, Q4; OR 0.51 95%CI 0.31-0.82) were associated with lower odds for frailty. Higher plant (∼0.60 g/kg BW/d [Q4 vs Q1: OR 0.35 95%CI 0.22-0.57]) and animal (up to ∼0.80 g/kg BW/d; [Q3 vs Q1: OR 0.72 95%CI 0.53-0.97] protein intakes, were associated with lower odds for frailty. Older women consuming higher total protein, including a combination of animal and plant sources, were less likely to be frail, with intakes of ∼1.1-1.6 g/kg likely to be most beneficial.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"238-247"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147591950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yazan Ranneh, Mohammed Hamsho, Abdulmannan Fadel, Seham Al Raish, Hadeel Ali Ghazzawi, Maha Hoteit, Gonzalo Saiz-Gonzalo
{"title":"Effect of dietary fibre supplementation on metabolic endotoxemia: a systematic review and meta-analysis of randomised clinical trials.","authors":"Yazan Ranneh, Mohammed Hamsho, Abdulmannan Fadel, Seham Al Raish, Hadeel Ali Ghazzawi, Maha Hoteit, Gonzalo Saiz-Gonzalo","doi":"10.1080/09637486.2026.2644867","DOIUrl":"10.1080/09637486.2026.2644867","url":null,"abstract":"<p><p>The objective of this systematic review and meta-analysis was to evaluate the impact of dietary fibre (DF) supplementation on metabolic endotoxemia (ME). A comprehensive search of multiple databases was conducted to identify relevant clinical trials. Selected trials were systematically reviewed, and a meta-analysis was performed using a random-effects model. The comprehensive database search retrieved 732 records; of these, 15 randomised controlled trials involving a total of 773 participants fulfilled the eligibility criteria and were subsequently included in the systematic review and meta-analysis. Supplementation with DF significantly decreased serum lipopolysaccharide levels (<i>p</i> < 0.00001, I<sup>2</sup> = 97%). TNF-α levels also decreased significantly (<i>p</i> < 0.02, I<sup>2</sup> = 90%), while IL-10 levels increased (<i>p</i> < 0.02, I<sup>2</sup> = 85%). DF supplementation did not significantly affect lipopolysaccharide-binding protein, C-reactive protein, IL-6, HDL, or LDL levels. The pooled evidence provides evidence to support the use of DF supplementation in ameliorating ME and inflammatory cytokines which could prevent chronic inflammation-related diseases.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"212-224"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
João Vitor Moraes-Santos, Josefina Bressan, Adriano Marçal Pimenta, Helen Hermana Miranda Hermsdorff, Patrícia Paula da Fonseca Grili, Valdete Regina Guandalini, Fernanda de Carvalho Vidigal, José Luiz Marques-Rocha
{"title":"Nutrient patterns associated with depression: evidence from the CUME+ study.","authors":"João Vitor Moraes-Santos, Josefina Bressan, Adriano Marçal Pimenta, Helen Hermana Miranda Hermsdorff, Patrícia Paula da Fonseca Grili, Valdete Regina Guandalini, Fernanda de Carvalho Vidigal, José Luiz Marques-Rocha","doi":"10.1080/09637486.2026.2654461","DOIUrl":"10.1080/09637486.2026.2654461","url":null,"abstract":"<p><p>The aim of the present study was to investigate the association between nutrient patterns and depression among participants of the Cohort of Universities of Minas Gerais Plus via an online questionnaire. Sociodemographic characteristics, health-related data, dietary intake, and a self-reported diagnosis of depression were analysed. Three nutrient patterns were identified using principal component analysis, with a cumulative variance of 43.7%. Multivariable-adjusted logistic regression analyses were performed. Among the 7.617 participants, 13.2% reported a diagnosis of depression. An animal-origin pattern was positively associated with depression among individuals classified as having normal weight (OR = 1.40; CI: 1.01-1.94). However, an anti-inflammatory pattern had a protective effect in the entire sample (OR = 0.754; CI: 0.599-0.948) as well as specific subgroups. These findings underscore the importance of promoting a healthy, adequate diet as a strategy for the prevention and management of depression among individuals with characteristics similar to those of the study population.</p>","PeriodicalId":14087,"journal":{"name":"International Journal of Food Sciences and Nutrition","volume":" ","pages":"259-270"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147722792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}