International Journal of Fertility & Sterility最新文献

{"title":"Nanocurcumin Decreases Nucleotide-Binding Oligomerization Domain-Like Receptor Family Pyrin Domain-Containing 3 Complex Expressions in An Experimental Testicular Torsion Model.","authors":"Negar Malekshahi Fard, Mohammad Bayat, Seyed Mohammad Jafar Haeri, Maryam Baazm","doi":"10.22074/ijfs.2024.2008608.1511","DOIUrl":"https://doi.org/10.22074/ijfs.2024.2008608.1511","url":null,"abstract":"<p><strong>Background: </strong>Testicular torsion is a surgical emergency leads to severe acute ischemia injuries, and may eventually cause male infertility. The nucleotide-binding oligomerization domain (NOD)-like receptor family pyrin domaincontaining 3 (NLRP3) inflammasome is involved in testicular torsion pathophysiology. The aim of this study was to evaluate the effects of nanocurcumin (nCur) on testicular tissue and the NLRP3 inflammasome components.</p><p><strong>Materials and methods: </strong>In this experimental study, male Wistar rats (n=36) were randomly divided into six equal groups: controls, ischemia-reperfusion (I/R), I/R+nCur (50 or 100 mg/kg thirty minutes before reperfusion), and I/ R+nCur (50 or 100 mg/kg thirty minutes before reperfusion and continued for seven days). The left testis was rotated 720 (2×360) degrees counterclockwise to induce testicular torsion. After two hours of ischemia, detorsion was performed. At the end of treatment, an orchiectomy was carried out. The testis histopathology and the mRNA levels of NLRP3, apoptosis-associated speck-like protein (<i>ASC</i>), and <i>Caspase-1</i> were evaluated.</p><p><strong>Results: </strong>Our results revealed that, testicular I/R had a detrimental effect on testis histology such as the number of spermatogonia (14.5 ± 0.57, P<0.001) and the seminiferous tubules epithelium thickness (28.5 ± 11.7, P=0.007). It also significantly increased the expression of the NLRP3 inflammasome components (P<0.001). Treatment with nCur (in both doses) improved testicular damage and significantly reduced the expression of <i>NLRP3</i> (P=0.007), ASC (P=0.003), and <i>Caspase-1</i> (P<0.001).</p><p><strong>Conclusion: </strong>These results imply that nCur might be a useful therapeutic strategy in the field of reproductive medicine to diminish the side effects of testicular I/R via its anti-inflammatory properties and may be employed as adjuvant therapy to lessen testicular torsion complications.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"18 4","pages":"411-416"},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laparoscopic and Hysteroscopic Findings in A Selected Group of Women with Unexplained Infertility: A Cross-Sectional Study.","authors":"Maryam Hashemi, Helma Mohseni, Ataallah Ghahiri, Safoura Rouholamin","doi":"10.22074/ijfs.2023.2005476.1484","DOIUrl":"https://doi.org/10.22074/ijfs.2023.2005476.1484","url":null,"abstract":"<p><strong>Background: </strong>The role of laparoscopy and hysteroscopy in managing unexplained infertility (UI) is debatable because of the improved success rate of assisted reproductive technologies (ART). This study aims to assess the findings of laparoscopy and hysteroscopy in selected women diagnosed with UI to determine the frequency of such pathological conditions in order to manage them properly.</p><p><strong>Materials and methods: </strong>The current cross-sectional study was conducted on 96 women who attended an infertility clinic at the educational hospitals of Isfahan University of Medical Sciences from March 2018 to February 2020. The participants had one or more of the following conditions: had failed to conceive after 2-3 cycles of ovulation induction with clomiphene citrate and intrauterine insemination (IUI), had a history of pelvic infection, pelvic surgery, or ectopic pregnancy (EP). Laparoscopy and hysteroscopic findings were recorded for all participants.</p><p><strong>Results: </strong>Fifty-nine (61.4%) women had primary infertility, while 37 (38.6%) suffered from secondary infertility. In patients with primary and secondary infertility, 42.3 and 43.2% had laparoscopic abnormalities, respectively. Additionally, 33.8 and 21.6% of the participants had hysteroscopic abnormalities in the primary and secondary groups, respectively. The most common findings in the two groups of infertility who had done laparoscopy were endometriosis (21.8%, P=0.201) followed by tubal pathology (13.5%, P=0.952). Also, the most common intrauterine pathology found in both groups were uterine septum (7.2%, P=0.753) and endometritis (6.2%, P=0.241).</p><p><strong>Conclusion: </strong>Based on the findings of this study, laparoscopy is recommended in UI after three failed IUI and ovarian stimulation, a history of pelvic pain, pelvic surgery, or pelvic infection; however, it seems that further investigation is required to recommend universal hysteroscopy to all women with UI. Nonetheless, it is still emphasized that regional practice in one's local settings may also be effective concerning the prevalence of these pathologies.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"18 4","pages":"339-343"},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining Factors Influencing The Successful Embryo Transfer and Pregnancy during The Frozen Cycle of <i>In Vitro</i> Fertilization: A Retrospective Cohort Study.","authors":"Chanakarn Suebthawinkul, Pranee Numchaisrika, Akarawin Chaengsawang, Vijakhana Pilaisangsuree, Sadanan Summat, Wisan Sereepapong","doi":"10.22074/ijfs.2023.2009998.1532","DOIUrl":"https://doi.org/10.22074/ijfs.2023.2009998.1532","url":null,"abstract":"<p><strong>Background: </strong>Frozen embryo transfer (FET) has been increasingly used due to advancements in cryopreservation techniques and the safety advantages. This study aims to determine various factors influencing the successful FET.</p><p><strong>Materials and methods: </strong>Retrospective cohort analysis included 1112 women who underwent programmed FET between January 2012 and October 2022, at King Chulalongkorn Memorial Hospital, Thailand. Patient characteristics, embryo characteristics, endometrial preparation protocol, endometrial characteristics (thickness, pattern), embryo transfer procedure (tip and flow during transfer, embryo placement location, the difficulty of the procedure, presence of blood and mucous at catheter), and operator factor were analyzed. Multiple logistic regression analysis was used to assess the relationship between collected variables and successful embryo transfer which is defined by clinical pregnancy.</p><p><strong>Results: </strong>The overall clinical pregnancy rate was 34.2%. Women aged 35-40 years and >40 years were less likely to have a clinical pregnancy compared to those aged <35 years [adjusted odds ratio (aOR): 0.523; 95% confidence intervals (CI): 0.360-0.757, P<0.001 and aOR: 0.260; 95% CI: 0.152-0.434, P<0.001, respectively]. Obese women with body mass index (BMI) ≥25 kg/m2 were significantly associated with decreased clinical pregnancy (aOR: 0.632; 95% CI: 0.403-0.978, P=0.042) compared to those with normal BMI. Day-3 and day-4 embryo transfer showed a significant decrease in clinical pregnancy compared to blastocyst transfer (aOR: 0.294; 95% CI: 0.173-0.485, P<0.001 and aOR: 0.497; 95% CI: 0.265-0.900, P=0.024). Double embryo transfer (DET) was 1.78 times more likely to have a clinical pregnancy than women with single embryo transfer (SET) (aOR: 1.779; 95% CI: 1.293-2.458, P<0.001). The cycles with endometrial thickness <8 mm were associated with a decrease in clinical pregnancy compared with those with a thickness ≥8 mm (aOR: 0.443; 95% CI: 0.225-0.823, P=0.013).</p><p><strong>Conclusion: </strong>Older age, obesity, non-blastocyst transfer, single embryo transfer, and endometrial thickness of <8 mm were significantly associated with a decreased clinical pregnancy in programmed FET.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"18 4","pages":"352-361"},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of L-Arginine for Preventing Preeclampsia and Improving Maternal and Neonatal Outcomes in High-Risk Pregnancies: A Systematic Review and Meta-Analysis.","authors":"Fatemeh Naderipour, Fariba Keshavarzi, Hosna Mirfakhraee, Parisa Dini, Niousha Jamshidnezhad, Nastaran Abolghasem, Foroogh Sabzghabaei, Fatemeh Shariatinia","doi":"10.22074/ijfs.2024.2016433.1580","DOIUrl":"https://doi.org/10.22074/ijfs.2024.2016433.1580","url":null,"abstract":"<p><p>Preeclampsia (PE) remains a significant cause of maternal and neonatal morbidity and mortality, particularly in high-risk pregnancies. The present study aims to assess the efficacy of L-arginine supplementation in preventing PE and improving maternal and neonatal outcomes in high-risk pregnancies. We searched international databases (ISI, PubMed, Scopus, and Embase) and extracted studies that evaluated the efficacy of L-arginine for preventing PE and improving maternal and neonatal outcomes in high-risk pregnancies. The data collected were analysed utilising the random-effects model in Stata (version 15). Out of the collected studies, 10 met the eligibility criteria, comprising a total sample size of 1165 subjects (586 cases and 584 controls). The mean age of the cases was 28 ± 5.05 years, while that of the controls was 27.32 ± 4.58 years. The results revealed that L-arginine was more effective in reducing the incidence of PE than placebo [odds ratio (OR)=0.36, 95% confidence interval (CI): 0.17, 0.77]. Also, the results showed no statistically significant difference between the two groups in systolic blood pressure (SBP; standard mean difference (SMD): -0.35, 95% CI: -91, 21) and diastolic blood pressure (DBP; SMD: -0.50, 95% CI: -1.08, 0.07). There was a statistically significant difference in neonatal birth weight between the two groups (SMD: -0.16, 95% CI: -31, -0.01). No statistically significant difference existed between the two groups in terms of gestational age (SMD: 0.6, 95% CI: -0.06, 0.18). Furthermore, there was no significant difference between the two groups in the APGAR score at one minute (SMD: 0.40, 95% CI: -0.02, 0.82). Our findings revealed that L-arginine supplementation during pregnancy reduced the incidence of PE in high-risk pregnancies. However, it does not significantly improve maternal and neonatal outcomes.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"18 4","pages":"323-328"},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Hepatitis B Virus Infection on Semen Quality of Infertile Men: A Systematic Review and Meta-Analysis of Observational Studies.","authors":"Elaheh Mansouri Ghezelhesari, Elnaz Iranifard, Atefeh Yas, Ali Taghipour, Maliheh Mahmoudinia, Robab Latifnejad Roudsari","doi":"10.22074/ijfs.2023.1987000.1421","DOIUrl":"https://doi.org/10.22074/ijfs.2023.1987000.1421","url":null,"abstract":"<p><p>Semen quality is considered as an alternative measure for men's fertility. Several studies have examined the association between the hepatitis B virus (HBV) infection and semen quality, but the findings have been inconclusive. This study investigated the effects of HBV on semen quality. This systematic review and meta-analysis was conducted according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) 2020, as well as Meta-analysis Of Observational Studies in Epidemiology (MOOSE) guideline. In this study, international electronic databases including PubMed (National Library of Medicine), Web of Science, Scopus and Cochrane Library and also Persian databases including Scientific Information Database (SID) and Magiran were searched without a time limit until June 2023. All cohort and case-control studies examining the effects of HBV infection on the semen parameters of infertile men were included in the study. Animal studies, letters to the editor and case reports were excluded. The Newcastle-Ottawa scale was used to evaluate the quality of the included articles. Data analysis was done with Stata software. Of the 913 studies, 13 observational studies comprised a total of 10156 patients were included in this review, nine of which were included in the meta-analysis. The results showed that HBV infection has a negative effect on semen volume (95% confidence interval (CI): [-0.33, -0.09], P<0.001), sperm concentration (95% CI: [-8.53, -2.66], Pv<0.001), sperm motility (95% CI: [-10.06, -3.39], P<0.001), sperm normal morphology (95% CI: [-6.42, -1.06], P<0.001) and sperm viability (95% CI: [-11.64, -0.31], P<0.001). These results suggest that the HBV infection has a negative effect on the semen quality in infertile men. Nonetheless, further research with more robust design is recommended.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"18 4","pages":"329-338"},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered Expression of Circulating <i>miR-223-3p</i> in Patients with Idiopathic Recurrent Pregnancy Loss: A Case and Control Study.","authors":"Parisa Ostad Hasanzadeh, Saeid Ghorbian, Jafar Mohseni, Sedigheh Abdollahi Fard, Changiz Amadizadeh","doi":"10.22074/ijfs.2023.2005351.1483","DOIUrl":"https://doi.org/10.22074/ijfs.2023.2005351.1483","url":null,"abstract":"<p><strong>Background: </strong>Circulating microRNAs (miRNAs or miRs) are key regulators of a wide array of biological processes that aberrantly express under physiological as well as pathological conditions. This study aims to determine whether altered expression of circulating <i>miR-223-3p</i> in plasma is associated with an increased risk of idiopathic recurrent pregnancy loss (iRPL), and if it could be a novel non-invasive biomarker for diagnosis of iRPL.</p><p><strong>Materials and methods: </strong>In this case and control study, Plasma samples were obtained from 60 women with a history of ≥ 2 consecutive miscarriages and 60 women with at least one full-term pregnancy and no history of miscarriage. The samples were used to assess the expression of circulating <i>miR-223-3p</i> by quantitative real-time polymerase chain reaction (qPCR) and determine the correlation between patient clinico-demographic parameters and miR-223-3p expression levels. We used receiver operating characteristic (ROC) curve analysis to evaluate the diagnostic accuracy of <i>miR-223-3p</i> for the diagnosis of iRPL.</p><p><strong>Results: </strong>There was significant up-regulation in <i>miR-223-3p</i> expression in patients with iRPL compared with healthy controls (P=0.002). No significant correlation was found between the expression level of <i>miR-223-3p</i> and the number of miscarriages in iRPL patients (P=0.344). ROC curve analysis revealed that the area under the curve (AUC) value for <i>miR-223-3p</i> was 0.658 [95% confidence interval (95% CI): 0.5590.757, P=0.002).</p><p><strong>Conclusion: </strong>These results suggest that a higher expression level of circulating <i>miR-223-3p</i> may be closely related with the increased risk of iRPL and possibly serve as a promising non-invasive diagnostic biomarker for iRPL.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"18 4","pages":"373-377"},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship between The Expression of Sperm <i>MicroRNA-149b</i> and <i>34c</i> and Sperm Quality in Men with Oligoasthenoteratozoospermia in Endometriosis.","authors":"Fatemeh Dujaili, Maryam Khoshsokhan Muzaffar, Rahil Jannatifar","doi":"10.22074/ijfs.2024.2013568.1555","DOIUrl":"https://doi.org/10.22074/ijfs.2024.2013568.1555","url":null,"abstract":"<p><strong>Background: </strong>The objective of this study was to investigate the correlation between the expression levels of sperm <i>microRNA-149b</i> and <i>34c</i> and sperm quality in men diagnosed with oligoasthenoteratozoospermia.</p><p><strong>Materials and methods: </strong>In the experimental study, we recruited 30 infertile men with oligoasthenoteratozoospermia and 30 control samples. In this study, <i>miR-149b</i> and <i>miR 34c</i> expression using reverse transcription polymerase chain reaction (RT-PCR) were evaluated. Semen samples were collected and subjected to initial analysis, including semen parameters, following the protocol provided by the World Health Organization (WHO). The levels of total antioxidant capacity (TAC) and malondialdehyde (MDA) were measured using an ELISA kit.</p><p><strong>Results: </strong>The expression levels of <i>miR-149b</i> and <i>miR-34c</i> were significantly downregulated in men with oligoasthenoteratozoospermia compared to age-matched normozoospermic men, as determined by RT-qPCR (P=0.001, and P=0.003 respectively). Correlation analysis revealed a positive correlation between the expression levels of m<i>miR-149b</i> and <i>miR-34c</i> and various sperm parameters, including sperm count, motility, morphology, viability, sperm mitochondrial membrane potential, sperm capacity, and TAC. Additionally, an inverse correlation was observed between the expression levels of <i>miR-149b, miR-34c,</i> DNA fragmentation, and MDA levels.</p><p><strong>Conclusion: </strong>The findings of this study indicate that the decreased expression of <i>miR-149b</i> and <i>miR-34c</i> is associated with oligoasthenoteratozoospermia, potentially affecting fundamental semen parameters. These results provide a basis for future research aimed at exploring potential therapeutic interventions for male infertility.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"18 4","pages":"404-410"},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Combination of Artificial Intelligence with Genetic Algorithms on Static Time-Lapse Images Improves Consistency in Blastocyst Assessment, An Interpretable Tool to Automate Human Embryo Evaluation: A Retrospective Cohort Study.","authors":"Marco Toschi, Lorena Bori, Jose Celso Rocha, Cristina Hickman, Marcelo Fabio Gouveia Nogueira, Andre Satoshi Ferreira, Murilo Costa Maffeis, Jonas Malmsten, Qiansheng Zhan, Nikica Zaninovic, Marcos Meseguer","doi":"10.22074/ijfs.2024.2008339.1510","DOIUrl":"https://doi.org/10.22074/ijfs.2024.2008339.1510","url":null,"abstract":"<p><strong>Background: </strong>In recent times, various algorithms have been developed to assist in the selection of embryos for transfer based on artificial intelligence (AI). Nevertheless, the majority of AI models employed in this context were characterized by a lack of transparency. To address these concerns, we aim to design an interpretable tool to automate human embryo evaluation by combining artificial neural networks (ANNs) and genetic algorithms (GA).</p><p><strong>Materials and methods: </strong>This retrospective cohort study included 223 human blastocyst time-lapse (TL) images taken at 110 hours post-injection. All the images were evaluated by five embryologists from different clinics in terms of blastocyst expansion (BE), quality of the inner cell mass (ICM), and trophectoderm (TE). The embryo database was used to develop an AI system (70% training, 15% validation, and 15% test) for automate blastocyst assessment. The entire set of images underwent a standardization process, followed by processing and segmentation using Matlab software. The resulting quantified variables were utilized in AI techniques (ANN and GA). Finally, the accuracy and performance of the automation tool was assessed with the area under the receiver operating characteristic (ROC) curve (AUC). Then, the level of agreement among embryologists and between embryologists and the AI system was compared with Kappa Index.</p><p><strong>Results: </strong>The overall agreement among embryologists was low (Kappa: 0.4 for BE; and 0.3 for TE and ICM). The AI tool achieved higher consistency (Kappa 0.7 for BE and ICM; and 0.4 for TE). The AI exhibited high accuracy in classifying BE (test 81.5%), ICM (test 78.8%), and TE (test 78.3%) and better performance for BE (AUC 0.888-0.956) than for ICM (AUC 0.605-0.854) and TE (AUC 0.726-0.769) assessment.</p><p><strong>Conclusion: </strong>Our AI tool highlighted the superior consistency of AI compared to human operators in grading blastocyst morphology. This research represents an important step towards fully automating objective embryo evaluation.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"18 4","pages":"378-383"},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Effects of Soy Isoflavones and Broccoli Extract on Oxidative Stress, Autophagy, and Apoptosis Gene Markers in Endometriosis.","authors":"Soudabeh Sabetian, Bahia Namavar Jahromi, Sina Vakili, Mohammad Samare-Najaf, Sarah Siahbani, Fatemeh Zal, Nader Tanideh, Mahintaj Dara, Zahra Khodabandeh","doi":"10.22074/ijfs.2023.1999395.1457","DOIUrl":"https://doi.org/10.22074/ijfs.2023.1999395.1457","url":null,"abstract":"<p><strong>Background: </strong>Endometriosis is an idiopathic gynecological condition affecting women with pelvic pain and infertility in reproductive ages. Due to preventive and therapeutic effects of soy isoflavones (SI) and broccoli extract (BE) on tumor angiogenesis, inflammation and oxidative stress and since endometriosis is accompanied by chronic inflammation, in this study, we aim to evaluate the potential role of these compounds on the pathological scores of endometriosis and also consider the expression level of the gene markers of autophagy, apoptosis, and oxidative stress in an endometriosis rat model.</p><p><strong>Materials and methods: </strong>In the present experimental study, 45 mature female Sprague- Dawley rats weighing 220 ± 20 g at the age of eight weeks with surgically induced endometriosis was divided into five groups and treated for six weeks with normal saline (control group), BE, SI, BE+SI by oral gavage, and also Diphereline intramuscularly. The histopathological scores of the endometrial implants (0, 1, 2, 3: no, poorly, moderately and well-preserved epithelial layers, respectively) and the mRNA expression level of Bcl-2, Bax, Caspase-3, Beclin-1, Lc3, and Sod within peritoneal tissue were compared among the groups.</p><p><strong>Results: </strong>Pathologic scores of the implants in the Diphereline (1.2 ± 0.27) and BE+SI (1.2 ± 0.41) groups were declined significantly in comparison with the control group (2.08 ± 0.44) (P≤0.001). In the endometriotic structures, the mRNA expression levels of our target genes were improved significantly (P≤0.01) in comparison with the control group.</p><p><strong>Conclusion: </strong>The findings of the current study demonstrated that the simultaneous consumption of a certain amount of broccoli extract and SI can be considered as a promising therapeutic strategy for treatment of endometriosis.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"18 4","pages":"384-390"},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Microfluidic Sperm Sorting on Embryonic Euploidy in Infertile Patients with Sperm DNA Damage: A Retrospective Study.","authors":"Sandra Lara-Cerrillo, Ana Raquel Jiménez Macedo, Olga Hortal, Candela Rosado Iglesias, Tania Lacruz Ruiz, Joan Carrera, Agustín García Peiró","doi":"10.22074/ijfs.2024.2007775.1499","DOIUrl":"https://doi.org/10.22074/ijfs.2024.2007775.1499","url":null,"abstract":"<p><strong>Background: </strong>Sperm DNA fragmentation is an important factor that affects male fertility. This study intends to evaluate the impact of sperm DNA damage [single-strand breaks (SSB) and double-strand breaks (DSB)] on fertilisation and embryonic euploidy after intracytoplasmic sperm injection (ICSI). Of the different sperm selection techniques, the novel microfluidic sperm sorting (MSS) ZyMōt™ ICSI device reduces both SSB and DSB in semen samples. The second objective is to study the impact of this MSS device on fertilisation and embryonic euploidy.</p><p><strong>Materials and methods: </strong>This retrospective study included data from 167 ICSI treatments. The alkaline and neutral Comet assays were used to analyse SSB and DSB, respectively. Analysis of fertilisation and embryo euploidy rates was performed in four groups of patients with normal/altered SSB or DSB values. Density gradient centrifugation (DGC) and the ZyMōt™ ICSI MSS device were used for semen preparation.</p><p><strong>Results: </strong>Fertilisation rates in 167 ICSI cycles were higher when using sperm from patients with normal SSB values (70.84%) and patients with abnormal SSB whose samples were processed using the MSS device (69.71%) compared to patients with abnormal SSB values using DGC (58.49%). Preimplantation genetic testing for aneuploidies (PGTA) revealed a higher embryo euploidy rate in patients with normal DSB values (60.00%) and patients with abnormal DSB whose samples were processed using the MSS device (44.59%) compared to patients with abnormal DSB values using DGC (36.84%). When female age was considered, there were fewer euploid embryos in women ≥35 years of age compared to younger women, independently of SSB and DSB. The number of euploid embryos increased when the MSS device was used.</p><p><strong>Conclusion: </strong>High SSB and DSB values in semen samples decreased fertilisation rates and embryonic euploidy, respectively. The ZyMōt™ ICSI device for semen preparation increased both rates, especially in couples that included women <35 years old.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"18 4","pages":"417-423"},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}