International Journal of Fertility & Sterility最新文献

{"title":"<i>miR-222</i> and <i>miR-146a</i> Variants in Idiopathic Male Infertility: A Case-Control Study.","authors":"Zahra Sadat Hoseini, Leila Kohan","doi":"10.22074/ijfs.2024.2029599.1691","DOIUrl":"10.22074/ijfs.2024.2029599.1691","url":null,"abstract":"<p><strong>Background: </strong>Idiopathic Male Infertility is a widespread problem affecting approximately 10 to 15 percent of men of reproductive age. Several genes have been studied related to idiopathic male infertility. Thereafter, among the family of non-coding RNAs, microRNAs are shown to play a very important role in the regulation of a subset of genes. It has been documented that infertile men have differentially expressed miRNA. The aim of this study is to evaluate the impact of two genetic variants, <i>miR-222</i> rs2858060 and <i>miR-146a</i> rs2910164 on idiopathic male infertility.</p><p><strong>Materials and methods: </strong>In this case-control study, Blood samples were taken from 201 men with idiopathic infertility and 201 men in a healthy state for this case-control investigation. Genotype determination of desired polymorphisms was done using the Tetra-ARMS PCR technique and data was analyzed by SPSS software version 20. Significant associations were determined at an alpha level of 0.05.</p><p><strong>Results: </strong>The study found a significant association between the <i>miR-222</i> rs2858060 GG genotype and idiopathic male infertility in the recessive genetic model [odds ratio (OR)=2.02, 95% confidence interval (CI)=1.25-3.24, P=0.004]. After adjusting for age, body mass index (BMI), smoking, and alcohol consumption, the association between the genotypes and the disease remained significant. No significant association was observed between miR-146a rs2910164 genotypes and male infertility in all genetic models (P>0.05). Moreover, haplotype analysis showed that the CC (rs2858060/ rs2910164) haplotype is associated with the decreased risk of idiopathic male infertility (OR=0.728, 95% CI =0.54-0.98, P=0.035).</p><p><strong>Conclusion: </strong><i>miR-222</i> rs2858060 and <i>miR-146a</i> rs2910164 polymorphisms influence the risk of male infertility in Iranian population. This is the first report to examine the role of <i>miR-222</i> rs2858060 and <i>miR-146a</i> rs2910164 in male infertility, further studies involving different ethnicity and larger sample sizes are needed to confirm our findings.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"19 3","pages":"284-289"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> Culture of Vitrified Immature Mouse Testicular Tissue in The Presence of N-acetylcysteine Antioxidant.","authors":"Parmiss Nikoosokhan, Zeinab Ghezelayagh, Samira Hajiaghalou, Alireza Alizadeh Moghadam Masouleh, Bita Ebrahimi","doi":"10.22074/ijfs.2025.2039641.1749","DOIUrl":"10.22074/ijfs.2025.2039641.1749","url":null,"abstract":"<p><strong>Background: </strong>Cryopreservation of immature testicular tissue is a suitable method for spermatogonial stem cell (SSC) preservation in prepubertal boys, who are at risk of infertility due to cancer treatments. Viable spermatozoa can be obtained by transplantation or <i>in vitro</i> culture of cryopreserved testicular tissue. Optimizing the culture conditions is essential for reducing tissue damage caused by oxidative stress produced during cryopreservation and culture. Our objective was to improve the culture conditions of vitrified immature mouse testicular tissue by using N-acetylcysteine (NAC) antioxidant.</p><p><strong>Materials and methods: </strong>In this experimental study, testicular tissues of 6-day-old immature NMRI mice were isolated, vitrified, and distributed into three groups: control, culture I (cultured without NAC), and culture II (cultured in the presence of 125 mM NAC). After seven days of culture, histological analysis, cell viability, apoptotic-related gene expression, promyelocytic leukaemia zinc finger (<i>Plzf</i>) gene expression, and Caspase-3 protein expression were assessed. Moreover, the malondialdehyde (MDA) level was measured in the culture media.</p><p><strong>Results: </strong>Tissue integrity and higher viability level were observed in the culture II group compared to the other two groups. Furthermore, the <i>Bax/Bcl-2</i> ratio and MDA level were decreased significantly in the culture ӀӀ group, whereas <i>Caspase-3</i> and <i>Plzf</i> gene expression were significantly increased.</p><p><strong>Conclusion: </strong>Our data revealed that the presence of 125 mM NAC improves the developmental process of vitrifiedwarmed immature mouse testicular fragments during <i>in vitro</i> culture, thus it may have potential implications for <i>in vitro</i> culturing of human prepubertal testicular tissues.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"19 3","pages":"296-304"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Vesicles Present in Bone Marrow Mesenchymal Stromal/Stem Cell Conditioned Media Restore Spermatogenesis in Azoospermic Mice.","authors":"Rano Zhankina, Afshin Zare, Alireza Afshar, Arezoo Khoradmehr, Mohammad Reza Dorvash, Farhad Rahmanifar, Nader Tanideh, Omid Koohi Hosseinabadi, Ali Arabi Monfared, Shahrokh Zare, Nadiar Maratovich Mussin, Asset Askerovich Kaliyev, Ulanbek Zhanbyrbekuly, Amin Tamadon","doi":"10.22074/ijfs.2024.2021445.1619","DOIUrl":"10.22074/ijfs.2024.2021445.1619","url":null,"abstract":"<p><p> Background: We aimed to examine the therapeutic efficacy of exosome-enriched conditioned media (CM), known for its high concentration of extracellular vesicles (EVs), in comparision with mesenchymal stromal/stem cells (MSCs) in treating non-obstructive azoospermia.</p><p><strong>Materials and methods: </strong>In this experimental study, we used adipose tissue-derived MSCs (AT-MSCs), bone marrowderived MSCs (BM-MSCs), and BMCM containing EVs to treat busulfan-induced azoospermia in animal models. The study included thirty adult male Balb/C mice and two female enhanced green fluorescent protein-positive (eGFP+/+) Balb/C mice for experimental groups and stem cell culturing. Groups consisted of an intact control, an azoospermia group, an AT-MSC therapy group, a BM-MSC therapy group, a BMCM therapy group, and a spontaneous healing group. Testes were removed from all mice, and histomorphometry and flow cytometry analyses were performed 60 days post-treatment. Additionally, protein structure extraction, protein-protein docking analysis, and data visualization were conducted.</p><p><strong>Results: </strong>Histomorphometry and flow cytometry showed that most seminiferous tubules in the therapy groups exhibited normal morphology and restored spermatogenesis, unlike the azoospermia group. In silico protein docking analysis revealed that exosome factors in BM-MSCs positively impacted spermatogenesis. The BM-MSC and BMCM therapy groups showed more favorable outcomes compared to other groups. Key exosome factors like Basigin, E3 ubiquitinprotein ligase (UBR2), transforming growth factor beta (TGF-β), hepatocyte growth factor (HGF), and programmed death-ligand 1 (PD-L1) interacted with receptors critical to this process.</p><p><strong>Conclusion: </strong>Our findings indicate that both BMCM enriched with EVs and the administration of AT-MSCs and BM-MSCs effectively induced spermatogenesis in mice with busulfan-induced azoospermia. Specifically, BM-MSC therapy exhibited superior outcomes compared to AT-MSCs and BMCM alone. This study highlights the potential of EV-based therapies, particularly BMCM, as a promising strategy for treating non-obstructive azoospermia. Furthermore, the interaction of key exosome factors with critical receptors enhances our understanding of the underlying molecular mechanisms involved in restoring reproductive function in testes.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"19 3","pages":"305-318"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12207096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PGT-M as A Family Planning Tool for A Couple in Which The Woman Carries A Novel NCAH Variant and A CYP21A2 Variant also Present in Her Husband: A Case Report.","authors":"Melissa Cavagnoli, Maria Augusta Tamm, Vickie White Loureiro Souza, Ana Cerveró, Taccyanna Mikulski Ali, Juliana Cuzzi, Márcia Riboldi, Maite Del Collado","doi":"10.22074/ijfs.2025.2032427.1716","DOIUrl":"10.22074/ijfs.2025.2032427.1716","url":null,"abstract":"<p><p>The hyperandrogenic non-classic congenital adrenal hyperplasia (NCAH) is an autosomal recessive disorder that can lead to a decrease in reproductive function in women due to alterations in hormone levels. Couples in which the woman is affected must investigate the occurrence of the mutation in the partner to assess the possibility of passing on the condition on their offspring. In such cases, genetic counseling plays a crucial role in educating the patient about the risk of having a child with the same disorder. Additionally, assisted reproduction techniques (ART), including preimplantation genetic testing for monogenic diseases (PGT-M), are important to increase the chances of the couple conceiving a healthy child. In this case report, we describe a multidisciplinary approach aimed at achieving two infants who appeared unaffected by the classic form of the condition. The couple carried variants in CYP21A2 gene, and the woman, affected by NCAH, presented a novel variant.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"19 3","pages":"337-343"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12207327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Value of Serum Cancer Antigen 125, Carcinoembryonic Antigen, Cancer Antigen 19-9, Anti Müllerian Hormone, White Blood Cell Count, Platelet Count, and Neutrophil to Lymphocyte Ratio in Endometriosis: A Retrospective Study.","authors":"Behnaz Nouri, Niki Talebian, Ladan Kharaz, Mahdi Falah Tafti","doi":"10.22074/ijfs.2024.2010957.1534","DOIUrl":"10.22074/ijfs.2024.2010957.1534","url":null,"abstract":"<p><strong>Background: </strong>Endometriosis is among the leading causes of morbidity in the female population worldwide. Currently, the definite diagnosis of endometriosis depends on laparoscopy as the gold-standard method. Potential biomarkers, such as inflammatory biomarkers, cancer antigens, and hormones, offer non-invasive alternatives. This study was designed to investigate the utility of some hematological markers, including white blood cell (WBC) count, neutrophil to lymphocyte ratio (NLR), platelet count, cancer antigen 19-9 (CA 19-9), and 125 (CA-125), carcinoembryonic antigen (CEA), and anti Müllerian hormone (AMH), as non-invasive methods for endometriosis diagnosis.</p><p><strong>Materials and methods: </strong>In this retrospective study, which was performed on 346 females, the case group consisted of 230 endometriosis patients. The data of 116 patients with benign tumors or other benign conditions such as Müllerian anomalies, were used as the control group. Receiver-operating characteristic (ROC) analysis was implemented to calculate specificities and sensitivities of CA-125, CA 19-9, CEA, NLR, WBC count, platelet (PLT) count, and AMH.</p><p><strong>Results: </strong>Significantly higher mean values were observed for CA-125, CA 19-9, WBC count, and NLR in the case group compared with the control group (P<0.001). The combination of NLR and CA-125 demonstrated the highest diagnostic performance with an area under the curve (AUC) of 0.903. However, the AUC for CA-125 (0.896) was lower and the value of 12.7 IU/mL was the most appropriate cut-off point (sensitivity=93.9%, specificity=60.9%). The cut-off value of 35 for CA-125 was also evaluated (sensitivity=61.4%, specificity=98.3%). The AUC for NLR was 0.699 and the best cut-off point was 1.5 (sensitivity=83.4%, specificity=52.6%).</p><p><strong>Conclusion: </strong>Combined measurement of CA-125 and NLR showed the highest performance in the diagnosis of endometriosis and can be considered as a diagnostic marker. However, it is necessary to conduct more research to evaluate the applicability of these biomarkers.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"19 3","pages":"259-263"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of Apoptosis by Ethanol, Methanol, and Ethyl Acetate Extracts from <i>Cyperus Rotundus'</i> Leaf on <i>In Vitro</i> Human Ovarian, Cervix, and Breast Cancer Cell Lines.","authors":"Mahdi Moradi Kooshkmeydani, Arezoo Khoradmehr, Ali Dehghani Firoozabadi, Alireza Tavassoli, Farzad Mohammad Rezazadeh, Gholamhossein Mohebbi, Asset A Kaliyev, Nadiar Maratovich Mussin, Mojtaba Farjam, Kulyash R Zhilisbayeva, Amin Tamadon","doi":"10.22074/ijfs.2024.2019125.1602","DOIUrl":"10.22074/ijfs.2024.2019125.1602","url":null,"abstract":"<p><strong>Background: </strong>Cervical, breast, and ovarian cancers exhibit significant incidence and fatality rates, necessitating diverse approaches for effective cancer cell eradication while preserving normal cells. The aim of this study is to explore the <i>in vitro</i> apoptosis-inducing properties of hydroalcoholic extracts from <i>Cyperus rotundus'</i> (C. rotundus) leaf using human gynecological cancer cell lines.</p><p><strong>Materials and methods: </strong>In this experimental study, Hydroalcoholic extracts were prepared from the leaf of C. rotundus using ethanol, methanol, and ethyl acetate. These extracts were applied to MCF-7, HeLa, OVCAR-3, and Vero cell lines at concentrations of 3.125, 6.25, 12.5, and 25 g/ml. MTT test, assessing inhibition of proliferation at 50% (IC₅₀), was employed to evaluate each extract's ability to inhibit cell proliferation. Subsequently, apoptosis-related gene and protein regulation were examined using reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis.</p><p><strong>Results: </strong>Gas chromatography-mass spectrometry (GC-MS) analysis revealed that the methanolic extract contained hexadecanoic acid and dodecanoic acid. The ethanolic extract was found to have norspermidine and desulphosinigrin. Additionally, the ethyl acetate extract included vitamin E, 1-heptatriacotanol, lupeol, betulin, stigmasterol, and stearic acid. The HeLa treatment group with 6.25 μg/ml of ethyl acetate extract, MCF-7, and OVCAR-3 cells with 3.125 μg/ ml of methanol extract treatment group exhibited the most significant growth inhibition in the MTT assay. Further analysis of these treatment groups revealed that the transcription and translation of BAX, Caspase-3, Caspase-8, and Caspase-9 increased overall, whereas Bcl-2 decreased in all cell lines.</p><p><strong>Conclusion: </strong>Hydroalcoholic extracts from <i>C. rotundus'</i> leaf may enhance the apoptosis of cancer cells by modulating the transcription, translation, and post-translation of proteins, with minimal impact on the growth and survival of non-cancerous cells.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"19 3","pages":"326-336"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12207328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pregnancy Outcomes of Frozen-Thawed Blastocysts versus Blastocysts Derived from Frozen-Thawed Cleavage Embryos: A Retrospective Study.","authors":"Huy Phuong Tran, Tuyet Thi-Diem Hoang, Ha Le-Bao Tran, Trang Nguyen-Khanh Huynh, Vy Nguyen-Thao Do, Chau Kim Mai, Son Truong Dang","doi":"10.22074/ijfs.2024.2036618.1739","DOIUrl":"10.22074/ijfs.2024.2036618.1739","url":null,"abstract":"<p><strong>Background: </strong>Limited data exist regarding the outcomes of frozen-thawed cleavage-stage embryos that undergo extended culture to reach the blastocyst stage. This study aimed to compare the pregnancy outcomes between two approaches: transferring blastocysts derived from frozen-thawed cleavage embryos (D3-5 group) and frozen-thawed blastocysts (D5 group).</p><p><strong>Materials and methods: </strong>This retrospective observational cohort analysis was conducted at Hung Vuong Hospital (CS/HV/24/23) from January 2022 to December 2023. The D3-5 group comprised 167 patients who underwent embryo transfer with frozen-thawed cleavage embryos, which were subsequently cultured for 2 days before being transferred as blastocysts. The D5 group included 342 patients who received frozen-thawed blastocysts. Positive human chorionic gonadotropin (hCG) rate, clinical pregnancy rate, ongoing pregnancy rate, live birth rate, pregnancy failure rate and cancellation rate were compared between the two groups.</p><p><strong>Results: </strong>In the D3-5 group, a significant proportion of cycles (65.3%) were cancelled, primarily due to the absence of developed blastocysts for transfer (85.3%), while the remaining 14.7% of cancellations were attributed to other reasons. Patients in the D3-5 group demonstrated comparable pregnancy outcomes to those in the D5 group: positive hCG rate (52 vs. 53%, P=0.898), clinical pregnancy rate (45 vs. 48%, P=0.785), ongoing pregnancy rate (34 vs. 33%, P=0.873), live birth rate (31 vs. 29%, P=0.839), and pregnancy failure rate (21 vs. 24%, P=0.656).</p><p><strong>Conclusion: </strong>The strategy of culturing frozen-thawed cleavage embryos for two days and transferring them as blastocysts is not inferior to the transfer of frozen-thawed blastocysts. It increases workload for embryologists and poses a risk of cycle cancellation. We propose that the use of frozen-thawed blastocysts may be a more efficient and patient-friendly option.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"19 3","pages":"264-270"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Dual Trigger on <i>In Vitro</i> Fertilization/Intracytoplasmic Sperm Injection Outcomes in Patients with Suboptimal Ovarian Response (POSEIDON Classification Group I): A Randomized Clinical Trial.","authors":"Maryam Hafezi, Arezoo Arabipoor, Maryam Zareei, Samira Vesali, Parisa Mostafaei, Nadia Zameni","doi":"10.22074/ijfs.2025.2027114.1669","DOIUrl":"10.22074/ijfs.2025.2027114.1669","url":null,"abstract":"<p><strong>Background: </strong>The study was conducted to investigate the effect of dual trigger with gonadotropin-releasing hormone agonist (GnRH-a) and standard dose human chorionic gonadotropin (hCG) on in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) outcomes in patients with a history of suboptimal ovarian response.</p><p><strong>Materials and methods: </strong>In this randomized clinical trial, 52 infertile women who were referred to Royan Institute from November 2019 to November 2022 for a second treatment with IVF/ICSI cycles, and had a suboptimal ovarian response [the Patient-Oriented Strategies Encompassing IndividualizeD Oocyte Number (POSEIDON) classification group I] in their previous cycle were evaluated. Ovulation stimulation was performed in all patients with the standard antagonist protocol. At the point of the final ovarian stimulation, patients were randomly assigned into two groups using the permuted block randomization method. In the dual trigger group, 0.2 mg of GnRH-a and two ampoules of recombinant hCG subcutaneously were administered to the patients at the same time. In control group, only two ampoules of recombinant hCG were injected subcutaneously. The ovarian stimulation outcomes and pregnancy rates were compared between groups utilizing appropriate statistical methods.</p><p><strong>Results: </strong>The two groups were homogeneous in terms of baseline characteristics. The statistical differences were found between groups in terms of, the total number of retrieved oocytes and the number of metaphase II (MII) oocytes as well as the number of obtained and frozen embryos in the dual trigger group were significantly more than those of in the control group (P=0.001, P=0.022, P=0.01, and P=0.035, respectively). The clinical pregnancy and live birth rates in the dual group were higher than those of the control group; nevertheless, the differences were not statistically significant (40.9 vs. 25% and 40.9 vs. 20%, P=0.275 and P=0.143, respectively).</p><p><strong>Conclusion: </strong>By virtue of these findings, dual trigger significantly improved the ovarian stimulation outcomes in the patients with a history of unexpected poor response. To validate the current findings, further clinical trials with larger sample sizes are required (registration number: NCT04549649).</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"19 3","pages":"251-258"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relation between Celiac Disease and Ovarian Reserve: A Case-Control Study.","authors":"Niloufar Mahmoudi, Sima Besharat, Mehrangiz Pichak, Somayeh Livani","doi":"10.22074/ijfs.2024.2019472.1604","DOIUrl":"10.22074/ijfs.2024.2019472.1604","url":null,"abstract":"<p><strong>Background: </strong>Celiac disease (CD) is one of the most prevalent chronic digestive disorders, often presenting with intestinal symptoms as well beyond. These symptoms may include reproductive complications. To gain a deeper understanding of a women's fertility potential, medical professionals employ sonographic and laboratory techniques to predict the ovarian reserve. The aim of this study was to explore the ovarian reserve in women affected by CD in comparison to healthy women in their reproductive years, this study aimed to find the relationship between CD and ovarian reserve.</p><p><strong>Materials and methods: </strong>This case-control study included a total of 27 patients diagnosed with CD, alongside an agematched group of 27 women without CD. A comprehensive checklist for demographic information was completed for each participant. During days 2-4 of the menstrual cycle, blood samples were collected to measure the levels of follicular stimulating hormones (FSH), luteinizing hormone (LH), thyroid stimulating hormone (TSH), anti-mullerin hormone (AMH), and prolactin. Simultaneously, pelvic ultrasound was conducted to assess the number of antral follicles count (AFC) and ovarian volume on the same day.</p><p><strong>Results: </strong>There was no significant difference in age, pregnancy history, spontaneous abortion, stillbirth, premature births, and low birth weight babies between the case and control groups (P>0.05). However, there were significant variations in mean weight, body mass index, and menarche age between the two groups (P<0.05). Moreover, the serum levels of FSH and AFC of the right and left ovaries also showed significant differences between the two groups (P<0.05). However, no significant difference was observed regarding AMH, LH, TSH, prolactin, and ovarian volumes (P>0.05).</p><p><strong>Conclusion: </strong>The present study showed that women with CD have significantly higher AFC and serum FSH levels than healthy women.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"19 3","pages":"271-276"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12207330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Study of <i>MTHFR</i> Methylation on Sperm Parameters in Infertile Males: A Case-Control Study.","authors":"Tayebeh Amjadian, Parichehreh Yaghmaei, Nasim Hayati Roodbari, Kheirollah Yari","doi":"10.22074/ijfs.2024.2025042.1646","DOIUrl":"10.22074/ijfs.2024.2025042.1646","url":null,"abstract":"<p><strong>Background: </strong>The important roles of aberrant DNA methylation on semen abnormality have been demonstrated. Alternatively, 5,10-Methylenetetrahydrofolate reductase (MTHFR) is a vital enzyme to regulate the sperm DNA methylation patterns. This study aimed to investigate the methylation of <i>MTHFR</i> at differentially methylated regions (DMR) and the correlation between sperm DNA methylation patterns with semen quality parameters for assessing reproductive health and fertility status.</p><p><strong>Materials and methods: </strong>In this case-control study, semen samples were collected from 30 infertile (asthenospermia and oligoasthenoteratospermia), and 15 healthy men. Following the modification of DNAs by sodium bisulfite treatment, the methylation status of the <i>MTHFR</i> gene at MDRs was evaluated by the quantitative methylation specific PCR (qMSP) method.</p><p><strong>Results: </strong>Men with oligoasthenoteratospermia showed a statistically significant difference in mean MTHFR-DMR methylation levels compared to controls (P=0.047) and asthenospermia (P=0.034). Moreover, significant trends of decreasing values were observed in all parameters of the ejaculate (sperm concentration, their overall motility or vitality, and morphology) in men with asthenospermia and oligoasthenoteratospermia. These findings suggest a potential association between increased MTHFR-DMR methylation and reduced semen quality such as spermatozoa count (P=0.002), spermatozoa concentration (P=0.003), progressive (P=0.019), and normal morphology (P=0.003).</p><p><strong>Conclusion: </strong>We found that abnormal DNA methylation of <i>MTHFR</i> at DMR region was correlated with decreased sperm parameters and therefore male infertility. Further research is needed to explore the mechanisms affecting of MTHFR methylation on male infertility.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":"19 3","pages":"290-295"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}