International Journal of Legal Medicine最新文献_第9页

Developmental validation of the STRSeqTyper122 kit for massively parallel sequencing of forensic STRs. 用于法医 STR 大规模并行测序的 STRSeqTyper122 试剂盒的开发验证。

IF 2.1 3区医学

International Journal of Legal Medicine Pub Date : 2024-07-01 Epub Date: 2024-02-28 DOI: 10.1007/s00414-024-03195-2

Li-Liang Guo, Jia-Hui Yuan, Chi Zhang, Jie Zhao, Yi-Ren Yao, Ke-Li Guo, Yang Meng, An-Quan Ji, Ke-Lai Kang, Le Wang

引用次数: 0

Identification of a murder caused by brodifacoum poisoning based on clinical examinations and LC-MS/MS results. 根据临床检查和 LC-MS/MS 结果鉴定溴鼠灵中毒谋杀案。

IF 2.1 3区医学

International Journal of Legal Medicine Pub Date : 2024-07-01 Epub Date: 2024-03-05 DOI: 10.1007/s00414-024-03198-z

Zhonghao Yu, Erjuan Zhao, Qing Shi, Yuhao Yuan, Jinghong Ma, Luwang Zhou, Yijie Duan, Yiwu Zhou

引用次数: 0

Dental age assessment and dental scoring systems: combined different statistical methods. 牙龄评估和牙科评分系统：结合不同的统计方法。

IF 2.1 3区医学

International Journal of Legal Medicine Pub Date : 2024-07-01 Epub Date: 2024-03-28 DOI: 10.1007/s00414-024-03216-0

Cristiana Palmela Pereira, Ana Rodrigues, Diana Augusto, Adriana Santos, Valon Nushic, Rui Santos

{"title":"Dental age assessment and dental scoring systems: combined different statistical methods.","authors":"Cristiana Palmela Pereira, Ana Rodrigues, Diana Augusto, Adriana Santos, Valon Nushic, Rui Santos","doi":"10.1007/s00414-024-03216-0","DOIUrl":"10.1007/s00414-024-03216-0","url":null,"abstract":"Introduction: Age Estimation has been considered as a human basic right, carried out through the use of tables for dental age assessment based on the chronology of tooth eruption. As such, the final aim of this investigation is to create tables with applicability to the Portuguese population, for the different scoring systems used and combined different statistical approaches.Materials and methods: For this purpose, dental age assessment was achieved in all four third molars, using different scoring systems, in a total sample of 626 orthopantomograms (324 females, 302 males), aged between 12 and 25 years old, from the database population of Lisbon North University Hospital Center, approved by the Ethic Committee.Results: The values of validation showed excellent results both on precision and on reproducibility. Mostly all methods showed statistically significant differences between the estimated age and the chronological age and, therefore, the presence of estimation errors. Kullman's and Mincer's methods are the ones with best applicability in the Portuguese population, in the lower third molars. The reliability measures (sensitivity, specificity and accuracy) values decrease as age increases.Conclusion: A combination of the scoring systems as a protocol for dental age assessment in Portuguese nationality was established. Tables, for all the scoring systems used, were made with applicability in the Portuguese population.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypothermic sepsis in time since death estimation - a case report. 估算死亡时间时的低温败血症--病例报告。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2024-07-01 Epub Date: 2024-02-20 DOI: 10.1007/s00414-024-03193-4

Stefan Potente, Victoria Hanser, Sara Heinbuch, Arne Wrede, Nadine Schäfer, Peter Schmidt

{"title":"Hypothermic sepsis in time since death estimation - a case report.","authors":"Stefan Potente, Victoria Hanser, Sara Heinbuch, Arne Wrede, Nadine Schäfer, Peter Schmidt","doi":"10.1007/s00414-024-03193-4","DOIUrl":"10.1007/s00414-024-03193-4","url":null,"abstract":"Both hyper- and hypothermia are problematic in temperature based forensic time since death estimation. Hyperthermia may occur in infection, traumatic brain injury, and intoxication. Hypothermia is encountered predominantly in exposure. Sepsis may present itself clinically as hypothermic. Sepsis is not uncommon in the forensic setting and mostly occurs in the context of malpractice accusations. There is usually little overlap between sepsis and typical forensic time since death estimation scenarios of violent or otherwise suspicious deaths. In the presented case, hypothermia and time since death estimations did collide. An inmate was found dead in his jail cell. Wardens claimed they had visually approached him alive relatively shortly prior. Rectal temperature measurements, using two separate crime scene thermometers as well as temperature loggers, revealed low rectal temperature at relatively high ambient temperature. These findings suggested a much longer postmortem interval and consequently raised doubts about the stated timeline. The wardens' claims were however confirmed by camera recordings, which also allowed a reasonable estimate of the true time of death. The cause of death was confirmed as septic organ failure at autopsy, which explained low rectal temperature. The presence of WISCHNEWSKI-spots was noted. When the PRISM-method was applied to the temperature recordings, low rectal temperature at the time of death was detected successfully. However, adaptation of the underlying equation for lower \"starting temperature\" did not produce satisfactory results. It is concluded that even though hypothermia at the time of death may possibly be detected from temperature data, attempts at time since death estimation for cases of hypothermia by adaptation of the equation should be avoided.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139905604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical analysis of PDGFR-α for wound age determination. 对 PDGFR-α 进行免疫组化分析，以确定伤口年龄。

IF 2.1 3区医学

International Journal of Legal Medicine Pub Date : 2024-07-01 Epub Date: 2024-03-23 DOI: 10.1007/s00414-024-03208-0

Wei Zhang, Yuko Ishida, Mizuho Nosaka, Yumi Kuninaka, Akiko Ishigami, Haruki Yasuda, Ayumi Kofuna, Ten Kamata, Utako Goto, Toshikazu Kondo

引用次数: 0

Development of a novel five dye insertion/deletion (INDEL) panel for ancestry determination. 开发用于确定祖先的新型五染插入/缺失（INDEL）面板。

IF 2.1 3区医学

International Journal of Legal Medicine Pub Date : 2024-07-01 Epub Date: 2024-02-19 DOI: 10.1007/s00414-024-03196-1

Lucio L Avellaneda, Damani T Johnson, Ryan M Gutierrez, Lindsey Thompson, Sarah A Sturm, Kelly A Sage, Rachel M Houston, Bobby L LaRue

{"title":"Development of a novel five dye insertion/deletion (INDEL) panel for ancestry determination.","authors":"Lucio L Avellaneda, Damani T Johnson, Ryan M Gutierrez, Lindsey Thompson, Sarah A Sturm, Kelly A Sage, Rachel M Houston, Bobby L LaRue","doi":"10.1007/s00414-024-03196-1","DOIUrl":"10.1007/s00414-024-03196-1","url":null,"abstract":"The use of genetic markers, specifically Short Tandem Repeats (STRs), has been a valuable tool for identifying persons of interest. However, the ability to analyze additional markers including Single Nucleotide Polymorphisms (SNPs) and Insertion/Deletion (INDELs) polymorphisms allows laboratories to explore other investigative leads. INDELs were chosen in this study because large panels can be differentiated by size, allowing them to be genotyped by capillary electrophoresis. Moreover, these markers do not produce stutter and are smaller in size than STRs, facilitating the recovery of genetic information from degraded samples. The INDEL Ancestry Informative Markers (AIMs) in this study were selected from the 1000 Genomes Project based on a fixation index (FST) greater than 0.50, high allele frequency divergence, and genetic distance. A total of 25 INDEL-AIMs were optimized and validated according to SWGDAM guidelines in a five-dye multiplex. To validate the panel, genotyping was performed on 155 unrelated individuals from four ancestral groups (Caucasian, African, Hispanic, and East Asian). Bayesian clustering and principal component analysis (PCA) were performed revealing clear separation among three groups, with some observed overlap within the Hispanic group. Additionally, the PCA results were compared against a training set of 793 samples from the 1000 Genomes Project, demonstrating consistent results. Validation studies showed the assay to be reproducible, tolerant to common inhibitors, robust with challenging casework type samples, and sensitive down to 125 pg. In conclusion, our results demonstrated the robustness and effectiveness of a 25 loci INDEL system for ancestry inference of four ancestries commonly found in the United States.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative evaluation of endometrium-expressed mRNAs for the purpose of discriminating between menstruation and traumatic vaginal injury in sexual assault cases. 定量评估子宫内膜表达的 mRNA，以区分性侵犯案件中的月经和外伤性阴道损伤。

IF 2.1 3区医学

International Journal of Legal Medicine Pub Date : 2024-07-01 Epub Date: 2024-02-27 DOI: 10.1007/s00414-024-03199-y

Tomoko Akutsu, Saki Minegishi, Ken Watanabe, Kochi Toyomane, Takayuki Yamagishi, Koichi Sakurada

{"title":"Quantitative evaluation of endometrium-expressed mRNAs for the purpose of discriminating between menstruation and traumatic vaginal injury in sexual assault cases.","authors":"Tomoko Akutsu, Saki Minegishi, Ken Watanabe, Kochi Toyomane, Takayuki Yamagishi, Koichi Sakurada","doi":"10.1007/s00414-024-03199-y","DOIUrl":"10.1007/s00414-024-03199-y","url":null,"abstract":"In sexual assault cases, it is crucial to discriminate between peripheral blood and menstrual blood to provide evidence for vaginal intercourse with traumatic injury. In this study, the menstrual blood mRNA markers progestagen-associated endometrial protein (PAEP), matrix metallopeptidase 7 (MMP7), and left-right determination factor 2 (LEFTY2) were evaluated by quantitative RT-PCR (RT-qPCR) for the discrimination of menstrual blood from peripheral blood and vaginal fluid. As a result, all markers with cutoff delta cycle quantification (ΔCq) values were specifically determined in menstrual blood among forensically relevant body fluids. Even though the changes in the expression levels of each marker differed during the menstrual cycle, all markers were determined to be positive in most of the randomly collected menstrual blood samples that were analyzed. Additionally, the markers with proposed cutoff ΔCq values could discriminate between menstrual blood and peripheral blood-mixed vaginal fluid samples. The determination of positive markers was less affected by storage temperature under dry conditions than under wet conditions, while PAEP was detectable in samples stored below room temperature under wet conditions. The detectability of PAEP was considered to be the result of its higher expression level compared with MMP7 and LEFTY2. In conclusion, menstrual blood markers for the RT-qPCR procedure evaluated in this study were highly specific for menstrual blood. The proposed procedure could be useful for discriminating between menstruation and traumatic bleeding in the female genital tract. In particular, PAEP is expected to be applicable to forensic casework samples because of its high specificity and robustness.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139971797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of metal ions and DNA recovery from the surface of fired and unfired brass ammunition to improve STR profiling. 评估已发射和未发射黄铜弹药表面的金属离子和 DNA 回收情况，以改进 STR 分析。

IF 2.1 3区医学

International Journal of Legal Medicine Pub Date : 2024-07-01 Epub Date: 2024-03-04 DOI: 10.1007/s00414-024-03200-8

Natalia Czado, Rachel Houston, Sheree Hughes

{"title":"Evaluation of metal ions and DNA recovery from the surface of fired and unfired brass ammunition to improve STR profiling.","authors":"Natalia Czado, Rachel Houston, Sheree Hughes","doi":"10.1007/s00414-024-03200-8","DOIUrl":"10.1007/s00414-024-03200-8","url":null,"abstract":"Interest in recovering DNA from the surface of ammunition evidence for genotyping has increased over the past few years. Numerous studies have examined a variety of methods to maximize DNA recovery from these types of challenging samples, but successful DNA profiling has been inconsistent. Low amounts of DNA and PCR inhibition due to metal ions have been suggested as the leading causes of poor results; however, no study quantitatively examined the presence of metal ions at various stages of the DNA analysis workflow from DNA collection through to amplification. In this study, the effectiveness of six different DNA collection and purification methods commonly used by forensic laboratories to process brass ammunition for DNA evidence was investigated. The amount of copper, zinc, and other metals co-recovered from fired and unfired brass casings during DNA collection (using numerous soaking, swabbing, and direct PCR protocols) was quantified via Inductively Coupled Plasma - Optical Emission Spectrometry (ICP-OES). This same panel of metals was subsequently quantified after DNA lysis and purification steps. Results demonstrated that low amounts of DNA, DNA damage, and degradation are more detrimental to STR typing results than PCR inhibition, as metal ions were successfully removed by all DNA purification methods tested. Furthermore, the use of metal ion chelators increased the amount of DNA recovered and number of reportable STR alleles. This research informs the forensic community on the most effective way to collect and process trace amounts of biological material from brass ammunition and similar evidence.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140021721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human identification: an investigation of 3D models of paranasal sinuses to establish a biological profile on a modern UK population. 人类识别：利用副鼻窦三维模型建立现代英国人口生物特征的研究。

IF 2.1 3区医学

International Journal of Legal Medicine Pub Date : 2024-07-01 Epub Date: 2024-02-13 DOI: 10.1007/s00414-024-03179-2

Madeline Robles, Sherry Nakhaeizadeh, Carolyn Rando, Ruth M Morgan

{"title":"Human identification: an investigation of 3D models of paranasal sinuses to establish a biological profile on a modern UK population.","authors":"Madeline Robles, Sherry Nakhaeizadeh, Carolyn Rando, Ruth M Morgan","doi":"10.1007/s00414-024-03179-2","DOIUrl":"10.1007/s00414-024-03179-2","url":null,"abstract":"Medical imaging is a valuable source for facilitating empirical research and provides an accessible gateway for developing novel forensic anthropological methods for analysis including 3D modelling. This is especially critical for the United Kingdom (UK), where methods developed from modern UK populations do not currently exist. This study introduces a new approach to assist in human identification using 3D models of the paranasal sinuses. The models were produced from a database of 500 modern CT scans provided by University College London Hospital. Linear measurements and elliptic Fourier coefficients taken from 1500 three-dimensional models across six ethnic groups assessed by one-way ANOVA and discriminant function analysis showed a range of classification rates with certain rates reaching 75-85.7% (p < 0.05) in correctly classifying age and sex according to size and shape. The findings offer insights into the potential for employing paranasal sinuses as an attribute for establishing the identification of unknown human remains in future crime reconstructions.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139729575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catch me if you can-emission patterns of human bodies in relation to postmortem changes. 如果可以，请抓住我--人体的发射模式与死后变化的关系。

IF 2.1 3区医学

International Journal of Legal Medicine Pub Date : 2024-07-01 Epub Date: 2024-03-08 DOI: 10.1007/s00414-024-03194-3

Alexandra Schieweck, Nicole Schulz, Jens Amendt, Christoph Birngruber, Franziska Holz

{"title":"Catch me if you can-emission patterns of human bodies in relation to postmortem changes.","authors":"Alexandra Schieweck, Nicole Schulz, Jens Amendt, Christoph Birngruber, Franziska Holz","doi":"10.1007/s00414-024-03194-3","DOIUrl":"10.1007/s00414-024-03194-3","url":null,"abstract":"The present study examines for the first time the emission patterns and olfactory signatures of 9 complete human corpses of different stages of decomposition. Air sampling was performed inside the body bags with solid sorbents and analysed by coupled gas chromatography-mass spectrometry after thermal desorption (TD-GC-MS). Furthermore, odour-related substances were detected by gas chromatography-olfactometry (GC-O). Sulfurous compounds (mainly dimethyl di- and trisulfide) were identified as most important to the odour perception. Around 350 individual organic substances were detected by TD-GC-MS, notably sulfurous and nitrogenous substances as well as branched alkanes, aldehydes, ketones, alcohols, carboxylic acids, carboxylic acid esters and ethers. A range of terpenes was detected for the first time in a characteristic emission pattern over all decomposition stages. Concentrations of the substances varied greatly, and no correlation between the emission patterns, the stage of decomposition and the cause of death could be found. While previous studies often analysed pig cadavers or only parts of human tissue, the present study shows the importance of analysing complete human corpses over a range of decomposition stages. Moreover, it is shown that using body bags as a kind of \"emission test chamber\" is a very promising approach, also because it is a realistic application considering the usual transport and store of a body before autopsy.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140059316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0