International Journal of Legal Medicine最新文献_第10页

Do forensic genetic markers disclose more information about us than they should? (A review). 法医基因标记是否透露了更多关于我们的信息？(审查)。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2025-01-08 DOI: 10.1007/s00414-024-03395-w

Carlota Manglano de la Fuente, Sara Palomo-Díez

{"title":"Do forensic genetic markers disclose more information about us than they should? (A review).","authors":"Carlota Manglano de la Fuente, Sara Palomo-Díez","doi":"10.1007/s00414-024-03395-w","DOIUrl":"https://doi.org/10.1007/s00414-024-03395-w","url":null,"abstract":"The 20 established STRs that make up the CoDIS package must comply with national and international privacy rights and legal policies. Current research reveals that it is possible that certain genetic markers, used in forensic contexts, may show information about other neighboring markers that could reflect certain private characteristics of individuals. Therefore, we will aim to find out, through a literature review, whether there may indeed be associations between some of the STRs alleles established by CoDIS and medical and phenotypic conditions, with the aim of checking whether this problem has a real basis. To carry out this review, a systematic search has been carried out in different databases, as well as a critical evaluation of the articles collected and a synthesis of all the relevant studies. The results generally show that private phenotypic data of the individual can be known from certain STRs established by CoDIS, due to their association with neighboring genes. However, most of the papers state that practically none of the established associations offer proof of causality, but rather a relationship between some STRs and a phenotype.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex estimation by CT image analysis of the rib cage in a Mediterranean population. 通过对地中海地区人群的肋骨CT图像分析进行性别估计。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2025-01-01 Epub Date: 2024-10-09 DOI: 10.1007/s00414-024-03349-2

Manuel Partido Navadijo, Jose Francisco Muñoz Molina, Fernando Navarro Merino, Inmaculada Alemán Aguilera

{"title":"Sex estimation by CT image analysis of the rib cage in a Mediterranean population.","authors":"Manuel Partido Navadijo, Jose Francisco Muñoz Molina, Fernando Navarro Merino, Inmaculada Alemán Aguilera","doi":"10.1007/s00414-024-03349-2","DOIUrl":"10.1007/s00414-024-03349-2","url":null,"abstract":"Sexual dimorphism in the human species is key to the development of sex estimation techniques in the human skeleton. This dimorphism is manifested, as in other regions of the skeleton, in the bones that constitute the thoracic cage, according to the existing bibliography. In this aspect, the study of the human skeleton through 3D images has also proved to be useful for the development and validation of sex estimation methodologies for the reconstruction of the osteobiological profile.For this purpose, a sample of 240 thoracic CT scans of adult individuals was selected from a collection of 3D images belonging to the University of Granada, provided by the Castilla-La Mancha Health Service (SESCAM). Different measurements of the thoracic bones (ribs R2 to R5 width, sternum length and width, and clavicles width) have been taken with OsiriX software, with the aim of developing discriminant functions for sex estimation.The obtained results are positive, allowing sex estimation through 3D images of the thorax with up to 89.6% accuracy through discriminant functions, which shows the usefulness of image analysis for the reconstruction of the osteobiological profile.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"419-427"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multidisciplinary approach to forensic biological profiling on a single tooth and nail sample. 对单一牙齿和指甲样本进行法医生物特征分析的多学科方法。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2025-01-01 Epub Date: 2024-10-25 DOI: 10.1007/s00414-024-03357-2

Rogier van der Hulst, Reza R R Gerretsen, Lisette M Kootker, Sanne W L Palstra, Arnoud J Kal, Saskia T M Ammer, Shirley P Verschoor, Lennaert C P Borra, Carina S M van Leeuwen, Miranda Verschraagen, Gareth R Davies, Roelof-Jan Oostra, Daniël J Touw

{"title":"A multidisciplinary approach to forensic biological profiling on a single tooth and nail sample.","authors":"Rogier van der Hulst, Reza R R Gerretsen, Lisette M Kootker, Sanne W L Palstra, Arnoud J Kal, Saskia T M Ammer, Shirley P Verschoor, Lennaert C P Borra, Carina S M van Leeuwen, Miranda Verschraagen, Gareth R Davies, Roelof-Jan Oostra, Daniël J Touw","doi":"10.1007/s00414-024-03357-2","DOIUrl":"10.1007/s00414-024-03357-2","url":null,"abstract":"Introduction: Analysis of a single tooth and nail can provide valuable forensic information, including year of birth, year of death, age, sex, DNA-profile, geographic residence during childhood and at time of death and drug exposure. The aim is to minimize the amount of used bodily material and to validate the applicability of a multidisciplinary sampling protocol.Methods: A nail of the big toe, a tooth and blood of seven deceased individuals were collected postmortem. Collected materials were sampled and segmented in accordance with the multidisciplinary sampling protocol. DNA analysis was conducted on the pulp of the tooth, isotope analysis (Sr, Pb, O and C) on the enamel and 14C-, toxicological and tooth cementum annulation analysis on root segments. DNA-, isotope (Sr, Pb, O and C) -, toxicological-, and 14C -analysis were conducted on toenail segments. The acquired DNA profiles were compared with profiles acquired from blood.Results: Material from seven deceased persons was analysed. 45 out of 56 analyses on dental samples were successful, constituting a success rate of 80%. Additionally, 27 out of 35 analyses were successful on nail samples, yielding a success rate of 77%. DNA-, toxicological and 14C- analyses performed better in nail than in tooth. Isotope analyses performed better in tooth than in nail. A profile with personal characteristics was constructed and matched for 62% of parameters with collected medical information.Conclusion: The performed sampling protocol for simultaneous multidisciplinary forensic analysis on a single tooth and nail sample provided applicable results and valuable information.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"361-374"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical forensic medicine in emergency departments: a pilot study of a forensic training and evaluation of its effectiveness in an Italian hospital. 急诊科的临床法医学：在意大利一家医院开展的法医培训试点研究及其效果评估。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2025-01-01 Epub Date: 2024-08-27 DOI: 10.1007/s00414-024-03313-0

Stefano Tambuzzi, Cecilia Rossi, Donatella Pavanello, Riccardo Primavera, Giorgio Costantino, Cristina Cattaneo

{"title":"Clinical forensic medicine in emergency departments: a pilot study of a forensic training and evaluation of its effectiveness in an Italian hospital.","authors":"Stefano Tambuzzi, Cecilia Rossi, Donatella Pavanello, Riccardo Primavera, Giorgio Costantino, Cristina Cattaneo","doi":"10.1007/s00414-024-03313-0","DOIUrl":"10.1007/s00414-024-03313-0","url":null,"abstract":"Because emergency departments are often the first point of contact for victims of violence, it is critical to provide the appropriate treatment in compliance with all necessary medicolegal precautions. For this reason, a randomized controlled trial was conducted at the Policlinico Hospital of Milan (Italy) in which an intervention group (12 physicians) received a 6-h course on clinical forensic medicine and their performance in medicolegal procedures in claimed cases of violence was compared with that of a control group (13 physicians) by means of a 16-item assessment scale over the 3 months before and the 3 months after the course. Overall, 195 medical records were included in the statistical analysis. Out of these cases, 105 occurred before the course (60 analyzed by the control group and 45 by the intervention group) and 90 occurred after the course (45 analyzed by the control group and 45 by the intervention group). The results showed that the overall mean score of physicians who participated to the course increased from 14.0 (IQR 7.0) to 19.0 (IQR 8.0) with a p-value < 0.0001 and that the comparison between the intervention group and the control group after the course was 19.0 (IQR = 8.0) and 14.0 (IQR = 7.0), respectively, with a p-value < 0.0001. The improvement was very little and below the expectations pointing out that educational courses, although they can be a first step towards raising the ED physicians' awareness of clinical forensics, may not be enough and that more structured training and new strategies should be implemented.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"275-284"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling STRs instability in a colorectal cancer FFPE sample: a case report. 揭示结直肠癌 FFPE 样本中 STRs 的不稳定性：一份病例报告。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2025-01-01 Epub Date: 2024-10-08 DOI: 10.1007/s00414-024-03341-w

Giulia Soldati, Chiara Saccardo, Dario Raniero, Domenico De Leo, Stefania Turrina

{"title":"Unveiling STRs instability in a colorectal cancer FFPE sample: a case report.","authors":"Giulia Soldati, Chiara Saccardo, Dario Raniero, Domenico De Leo, Stefania Turrina","doi":"10.1007/s00414-024-03341-w","DOIUrl":"10.1007/s00414-024-03341-w","url":null,"abstract":"In forensic genetics, sometimes formalin-fixed paraffin-embedded (FFPE) biopsy material taken during life is the only biological sample available for individual identification or paternity testing. In most cases, this biological tissue is characterized by the presence of tumor cells characterized by instability and loss of heterozygosity of microsatellites (MSI/LOH) compared to the DNA present in cells of normal tissue.In this case report, two FFPE samples from the same male subject were available for genetic investigation: one sample with colorectal cancer tissue and the other with normal tissue (no cancerous histopathological features). The comparison of the genetic profiles obtained from DNA extracted from the two tissues showed in the tumor tissue the presence of three genomic instability phenomena affecting FGA, CSF1P0, D21S2055 loci, located on three distinct autosomal chromosomes, and one duplication phenomenon affecting the DYS438. Therefore, due to the MSI/LOH phenomena, the genetic profile acquired from the tumor tissue was distorted and thus generated a fictitious genetic profile, not corresponding to the subject's real one (normal tissue free of tumor cells).","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"61-65"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of commercial targeted amplicon sequencing assays for human remains identification casework. 比较用于人类遗骸鉴定案例工作的商业定向扩增片段测序测定。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2025-01-01 Epub Date: 2024-10-15 DOI: 10.1007/s00414-024-03335-8

Dennis McNevin, Jessica Watson, Kelly Grisedale, Ayusha Dahal, Corey Goodwin, Jodie Ward

{"title":"Comparison of commercial targeted amplicon sequencing assays for human remains identification casework.","authors":"Dennis McNevin, Jessica Watson, Kelly Grisedale, Ayusha Dahal, Corey Goodwin, Jodie Ward","doi":"10.1007/s00414-024-03335-8","DOIUrl":"10.1007/s00414-024-03335-8","url":null,"abstract":"Targeted amplicon sequencing (TAS) facilitates the genotyping of forensically informative single nucleotide polymorphisms (SNPs) using massively parallel sequencing (MPS). For human remains identification, where any extracted DNA is likely to be degraded, TAS may succeed when short tandem repeat (STR) profiling using capillary electrophoresis fails. Further, as well as yielding identity information, SNPs can provide information about ancestry, phenotype, kinship and paternal lineage (Y chromosome haplotypes). Two TAS platforms were compared in this study: Ion AmpliSeq™ panels coupled with Ion Torrent sequencing on an Ion GeneStudio™ S5 Plus System, manufactured by Thermo Fisher Scientific, and the ForenSeq® Kintelligence Kit coupled with Illumina sequencing on the MiSeq FGx® Sequencing System, manufactured by QIAGEN. Four Ion AmpliSeq™ panels (Precision ID Identity, Precision ID Ancestry, DNA Phenotyping and HID Y-SNP) share 177 SNPs with the ForenSeq® Kintelligence Kit and all five were used to profile the DNA extracted from the petrous part of the temporal bone from six skeletonised cadavers. Of the 6 × 177 = 1,062 SNP genotype comparisons, 1,055 (99%) were concordant between the Ion AmpliSeq™ panels and Kintelligence Kit. Of the seven (< 1%) non-concordant SNPs, only three of them (0.3%) would have resulted in erroneous genotypes being reported as a result of allele dropout by either assay, using our optimised relative variant frequency windows for allele calling. We conclude that both the Ion AmpliSeq™ panels and the ForenSeq® Kintelligence Kit were suitable for TAS applied to the human remains in this study.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"49-60"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First evidence in an oversea French department of the deadly risk of protonitazene use: about 5 post mortem cases. 在法国海外省首次发现使用质子他嗪的致命风险：约 5 例尸检病例。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2025-01-01 Epub Date: 2024-08-22 DOI: 10.1007/s00414-024-03309-w

Christophe Maruejouls, Alice Ameline, Laurie Gheddar, Cédric Mazoyer, Kati Teston, Frédéric Aknouche, Pascal Kintz

{"title":"First evidence in an oversea French department of the deadly risk of protonitazene use: about 5 post mortem cases.","authors":"Christophe Maruejouls, Alice Ameline, Laurie Gheddar, Cédric Mazoyer, Kati Teston, Frédéric Aknouche, Pascal Kintz","doi":"10.1007/s00414-024-03309-w","DOIUrl":"10.1007/s00414-024-03309-w","url":null,"abstract":"Protonitazene is a synthetic benzoimidazole opioid of the nitazenes class, developed in the 1950s as an effective analgesic, but never released in the market due to severe side effects and major risk of dependence. The laboratory was involved in the determination of the cause of death for 5 subjects deceased in a French department of the Indian Ocean. The 5 victims were male, aged between 20 and 35 years. The first 2 victims were found dead in their prison cell and the 3 other victims were found dead in a squat. Therefore, we have developed and validated a specific procedure to identify and quantify the drug in post mortem specimens using LC-MS/MS. The procedure involves extraction of 0.5 mL fluid at pH 9.5 with a mixture of organic solvents in presence of 20 ng fentanyl-d5 used as internal standard. Linearity of the method was verified from 0.1 to 20 ng/mL in both whole blood and urine (r2 = 0.9983 and 0.9993, respectively). The limit of detection was estimated at 0.05 ng/mL in each matrix. Protonitazene was identified at < LOQ to 0.8 ng/mL, 0.4 to 2.9 ng/mL and 3.0 to 8.0 ng/mL in femoral blood, urine and bile, respectively. Post mortem concentrations were very low, which is consistent with reported high toxicity of protonitazene. As nitazenes represent a growing threat to public health in various parts of the world, this method seems to be a good response to the challenges posed by the identification of this class of substances.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"87-93"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic review on the use of C-reactive protein in autopsy practice. 关于在尸检实践中使用 C 反应蛋白的系统综述。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2025-01-01 Epub Date: 2024-09-20 DOI: 10.1007/s00414-024-03328-7

Asyraff Md Najib, Khairunnisa' Md Yusof, Wan Muhammad Azfar Wan Shuaib, Mohamed Swarhib Shafee, Faridah Mohd Nor

{"title":"A systematic review on the use of C-reactive protein in autopsy practice.","authors":"Asyraff Md Najib, Khairunnisa' Md Yusof, Wan Muhammad Azfar Wan Shuaib, Mohamed Swarhib Shafee, Faridah Mohd Nor","doi":"10.1007/s00414-024-03328-7","DOIUrl":"10.1007/s00414-024-03328-7","url":null,"abstract":"Postmortem analysis of C-reactive protein (CRP) in autopsies has been extensively researched for its potential utility. This analysis could aid forensic pathologists in screening for and investigating the potential infectious or inflammatory causes of death, thereby guiding appropriate autopsy procedures. To assess the diagnostic accuracy of postmortem CRP analysis in autopsy settings, a thorough electronic literature search was conducted across databases such as PubMed, Scopus, Web of Science, and Cochrane Library. Two independent reviewers screened eligible studies, followed by a methodological quality assessment using the QUADAS-2 checklist. Utilising a random-effects model, hierarchical summary receiver operating characteristic (HSROC) curve analysis and bivariate model meta-analysis were performed to evaluate heterogeneity across studies. Of the 1286 studies initially identified, nine met the eligibility criteria for the final analysis. The pooled sensitivity of postmortem CRP analysis was 0.93 (95% CI, 0.76, 0.98), with a pooled specificity of 0.80 (95% CI, 0.71, 0.87). The prevalence across studies ranged from 0.23 to 0.68, with a median of 0.5. Moderate variability was observed in the heterogeneity assessment across the primary studies. In summary, the study findings indicate that postmortem serum CRP analysis demonstrates high diagnostic accuracy with moderate heterogeneity. Additionally, postmortem CRP testing may be useful as a screening tool in autopsy practice to rule out the likelihood of sepsis.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"253-265"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A statistical evaluation of the sexual dimorphism of the acetabulum in an Iberian population. 对伊比利亚人髋臼性别二态性的统计评估。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2025-01-01 Epub Date: 2024-09-27 DOI: 10.1007/s00414-024-03334-9

Varsha Warrier, Marta San-Millán

{"title":"A statistical evaluation of the sexual dimorphism of the acetabulum in an Iberian population.","authors":"Varsha Warrier, Marta San-Millán","doi":"10.1007/s00414-024-03334-9","DOIUrl":"10.1007/s00414-024-03334-9","url":null,"abstract":"Sex estimation is essential for human identification within bioarchaeological and medico-legal contexts. Amongst the sexually dimorphic skeletal elements commonly utilised for this purpose, the pelvis is usually preferred because of its direct relationship with reproduction. Furthermore, the posterior part of the innominate bone has proven to have better preservation within degraded contexts. With the aim of investigating the potential of the vertical acetabular diameter as a sex marker, 668 documented individuals from three different Iberian skeletal collections were randomly divided into training and test samples and eventually analysed using different statistical approaches. Two traditional (Discriminant Function Analysis and Logistic Regression Analysis) and four Machine learning methodologies (Support Vector Classification, Decision Tree Classification, k Nearest Neighbour Classification, and Neural Networks) were performed and compared. Amongst these statistical modalities, Machine Learning methodologies yielded better accuracy outcomes, with DTC garnering highest accuracy percentages of 83.59% and 89.85% with the sex-pooled and female samples, respectively. With males, ANN yielded highest accuracy percentage of 87.70%, when compared to other statistical approaches. Higher accuracy obtained with ML, along with its minimal statistical assumptions, warrant these approaches to be increasingly utilised for further investigations involving sex estimation and human identification. In this line, the creation of a statistical platform with easier user interface can render such robust statistical modalities accessible to researchers and practitioners, effectively maximising its practical use. Future investigations should attempt to achieve this goal, alongside examining the influence of factors such as age, on the obtained accuracy outcomes.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"393-409"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Petrous bones versus tooth cementum for genetic analysis of aged skeletal remains. 用于老年骨骼遗骸基因分析的石质骨骼与牙骨质。

IF 2.2 3区医学

International Journal of Legal Medicine Pub Date : 2025-01-01 Epub Date: 2024-10-12 DOI: 10.1007/s00414-024-03346-5

Irena Zupanič Pajnič, Tonja Jeromelj, Tamara Leskovar

{"title":"Petrous bones versus tooth cementum for genetic analysis of aged skeletal remains.","authors":"Irena Zupanič Pajnič, Tonja Jeromelj, Tamara Leskovar","doi":"10.1007/s00414-024-03346-5","DOIUrl":"10.1007/s00414-024-03346-5","url":null,"abstract":"A proper sampling strategy is important to obtain sufficient DNA for successful identification of aged skeletal remains. The petrous bone is the highest DNA-yielding bone in the human body. Because DNA extraction from the petrous bone is very destructive, the demand for other DNA sources is significant. When investigating aged skeletal remains, teeth are usually preserved, and recent studies have shown that DNA in teeth can be best preserved in the dental cementum that surrounds the surface of the tooth root. To extract DNA from the surface of the tooth root, a nondestructive method without grinding was used. Petrous bones and teeth from 60 archaeological adult skeletons were analyzed. The DNA yield, degree of DNA degradation, and STR typing success were compared, and the results showed higher DNA yield and higher amplification success in petrous bones, despite higher degradation of petrous bones' DNA. The greater success of petrous bones is associated with poorly preserved DNA in a quarter of the teeth analyzed. When teeth with badly preserved DNA were excluded from the statistical analysis, no differences in the success of STR loci amplification were observed even if DNA yield was higher in petrous bones, which can be explained by greater degradation of petrous bones' DNA. When teeth are well preserved, they can be used for genetically analyzing aged skeletal remains instead of petrous bones, and a rapid nondestructive extraction method can be applied to shorten the identification process and to physically preserve the biological specimen.","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"41-48"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0