International Journal of Legal Medicine最新文献

{"title":"A statistical evaluation of the sexual dimorphism of the acetabulum in an Iberian population.","authors":"Varsha Warrier, Marta San-Millán","doi":"10.1007/s00414-024-03334-9","DOIUrl":"10.1007/s00414-024-03334-9","url":null,"abstract":"<p><p>Sex estimation is essential for human identification within bioarchaeological and medico-legal contexts. Amongst the sexually dimorphic skeletal elements commonly utilised for this purpose, the pelvis is usually preferred because of its direct relationship with reproduction. Furthermore, the posterior part of the innominate bone has proven to have better preservation within degraded contexts. With the aim of investigating the potential of the vertical acetabular diameter as a sex marker, 668 documented individuals from three different Iberian skeletal collections were randomly divided into training and test samples and eventually analysed using different statistical approaches. Two traditional (Discriminant Function Analysis and Logistic Regression Analysis) and four Machine learning methodologies (Support Vector Classification, Decision Tree Classification, k Nearest Neighbour Classification, and Neural Networks) were performed and compared. Amongst these statistical modalities, Machine Learning methodologies yielded better accuracy outcomes, with DTC garnering highest accuracy percentages of 83.59% and 89.85% with the sex-pooled and female samples, respectively. With males, ANN yielded highest accuracy percentage of 87.70%, when compared to other statistical approaches. Higher accuracy obtained with ML, along with its minimal statistical assumptions, warrant these approaches to be increasingly utilised for further investigations involving sex estimation and human identification. In this line, the creation of a statistical platform with easier user interface can render such robust statistical modalities accessible to researchers and practitioners, effectively maximising its practical use. Future investigations should attempt to achieve this goal, alongside examining the influence of factors such as age, on the obtained accuracy outcomes.</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"393-409"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Petrous bones versus tooth cementum for genetic analysis of aged skeletal remains.","authors":"Irena Zupanič Pajnič, Tonja Jeromelj, Tamara Leskovar","doi":"10.1007/s00414-024-03346-5","DOIUrl":"10.1007/s00414-024-03346-5","url":null,"abstract":"<p><p>A proper sampling strategy is important to obtain sufficient DNA for successful identification of aged skeletal remains. The petrous bone is the highest DNA-yielding bone in the human body. Because DNA extraction from the petrous bone is very destructive, the demand for other DNA sources is significant. When investigating aged skeletal remains, teeth are usually preserved, and recent studies have shown that DNA in teeth can be best preserved in the dental cementum that surrounds the surface of the tooth root. To extract DNA from the surface of the tooth root, a nondestructive method without grinding was used. Petrous bones and teeth from 60 archaeological adult skeletons were analyzed. The DNA yield, degree of DNA degradation, and STR typing success were compared, and the results showed higher DNA yield and higher amplification success in petrous bones, despite higher degradation of petrous bones' DNA. The greater success of petrous bones is associated with poorly preserved DNA in a quarter of the teeth analyzed. When teeth with badly preserved DNA were excluded from the statistical analysis, no differences in the success of STR loci amplification were observed even if DNA yield was higher in petrous bones, which can be explained by greater degradation of petrous bones' DNA. When teeth are well preserved, they can be used for genetically analyzing aged skeletal remains instead of petrous bones, and a rapid nondestructive extraction method can be applied to shorten the identification process and to physically preserve the biological specimen.</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"41-48"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postmortem interval estimation of human skeletonized remains through luminol chemiluminescence.","authors":"Catarina Ermida, Joana Rosa, Eugénia Cunha, Maria Teresa Ferreira","doi":"10.1007/s00414-024-03343-8","DOIUrl":"10.1007/s00414-024-03343-8","url":null,"abstract":"<p><p>Postmortem interval (PMI) estimation represents a significant challenge in the forensic sciences, particularly when dealing with human skeletal remains. A screening protocol for distinguishing possible remains of forensic interest is a crucial tool for judicial purposes. In this context, luminol chemiluminescence emerges as a promising method, with low overall costs and required time. This method is primarily used as a presumptive test, based on the understanding that the intensity of the chemiluminescence reactions decreases with an increase in the postmortem interval, thus underlining its practical implications.This research aims to expand previous research on the potential of luminol chemiluminescence, evaluating its usefulness in estimating PMI. Our sample comprised 239 human clavicles, with known PMI. The luminol solution was sprayed on each powder bone sample in a dark room, observed by the naked eye and photographed. The intensity of the chemiluminescence reaction was measured using a binary and a 5-level scale.The present results reveal that this method is a suitable tool for PMI estimation as a presumptive test, reducing time and costs in criminal investigations. The findings underscore the high sensitivity of luminol chemiluminescence for detecting recent PMI but also highlight a notable incidence of false positives. Thus, our results confirm luminol chemiluminescence as a powerful tool for dating time of death, particularly for identifying forensic relevant remains. Still, the relatively low specificity indicates that it should be complemented with additional tests for further confirmation and scientific validation of the remains' forensic relevance.</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"429-440"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery unbalanced DNA mixtures and evaluation mixing ratio via a droplet digital PCR method.","authors":"Xiaoyi Fu, Xueyun Li, Yuepeng Wang, Mingkun Xie, Dan Wen, Xuan Tang, Chudong Wang, Hongtao Jia, Yi Liu, Siqi Chen, Yue Wang, Lagabaiyila Zha, Jienan Li","doi":"10.1007/s00414-024-03306-z","DOIUrl":"10.1007/s00414-024-03306-z","url":null,"abstract":"<p><strong>Background: </strong>Small amounts of DNA from a perpetrator collected during crime-scene investigations can be masked by large amounts of DNA from the victim. These samples can provide important information for the perpetrator's conviction. Short tandem repeat (STR) detection system is not sensitive enough to detect trace amounts of minor components in unbalanced mixed DNA. We developed a system using droplet digital polymerase chain reaction (ddPCR) capable of discovering trace components and accurately determining the ratio of mixed DNA in extremely unbalanced mixtures.</p><p><strong>Methods: </strong>The non-recombining regions of the X chromosome and Y chromosome were quantified in the DNA of male and female mixtures using duplex ddPCR. Absolute quantification of low-abundance portions of trace samples and unbalanced mixtures was done using different mixing ratios.</p><p><strong>Results: </strong>The ddPCR system could be used to detect low-abundance samples with < 5 copies of DNA components in an extremely unbalanced mixture at a mixing ratio of 10000:1. The high sensitivity and specificity of the system could identify the mixing ratio of mixed DNA accurately.</p><p><strong>Conclusions: </strong>A ddPCR system was developed for evaluation of mixed samples of male DNA and female DNA. Our system could detect DNA quantities as low as 5 copies in extremely unbalanced mixed samples with good specificity and applicability. This method could assist forensic investigators in avoiding the omission of important physical evidence, and evaluating the ratio of mixed male/female trace samples.</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"67-76"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: A probability model for estimating age in young individuals relative to key legal thresholds: 15, 18 or 21-year.","authors":"Nina Heldring, Ali-Reza Rezaie, André Larsson, Rebecca Gahn, Brita Zilg, Simon Camilleri, Antoine Saade, Philipp Wesp, Elias Palm, Ola Kvist","doi":"10.1007/s00414-024-03347-4","DOIUrl":"10.1007/s00414-024-03347-4","url":null,"abstract":"","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"457"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Questionnaire survey for the International Journal of Legal Medicine on the occassion of the 26th triennal meeting of the International Academy of Legal Medicine.","authors":"Andreas Schmeling, Tony Fracasso","doi":"10.1007/s00414-024-03310-3","DOIUrl":"10.1007/s00414-024-03310-3","url":null,"abstract":"<p><p>A questionnaire was prepared in advance of the 26th triennial conference of the International Academy of Legal Medicine (IALM) and sent to 474 email addresses included in the IALM mailing list. The questionnaire addressed three current challenges faced by the International Journal of Legal Medicine (IJLM): the publication of guidelines and validation studies in the field of legal medicine, the publication ethics of case reports, and the recruitment of new reviewers for the IJLM. The response rate was 20%. The survey results highlight the need for international guidelines in various areas of legal medicine. Some desired guidelines already exist. To provide visibility and knowledge of the existing national guidelines, the IJLM has launched a Topical Collection on Quality Assurance in Legal Medicine. This collection aims to inform readers about country-specific characteristics of legal medicine structures and the existing national guidelines.Around 80% of the participants stated that there are legal or ethical requirements for the publication of forensic case reports or case series. Various options for obtaining consent for publication are discussed. Eighty-six of the 97 participants indicated their willingness to review manuscripts for the IJLM. It is emphasized that the contributions of reviewers should be duly recognized and valued.</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"285-291"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postmortem study of adrenomedullin and cortisol in femoral serum and pericardial fluid related to acute pulmonary edema.","authors":"Daniel Martínez-Jiménez, Juan Pedro Hernández Del Rincón, Maria Sabater-Molina, Cristina Pérez-Martínez, Carmen Torres, María D Pérez-Cárceles, Aurelio Luna","doi":"10.1007/s00414-024-03337-6","DOIUrl":"10.1007/s00414-024-03337-6","url":null,"abstract":"<p><p>Currently, various tools aid in determining the cause of death and the circumstances surrounding it. Thanatochemistry is one such method that provides insights into the physiopathological mechanisms of death and the behavior of specific biomarkers in different body fluids postmortem. Certain biomarkers, characterized by their stability and specificity to vital tissues like the lungs, are associated with mechanisms contributing to death, such as acute pulmonary edema (APE). This study aims to analyze the behavior of midregional pro-adrenomedullin (MR-proADM) and cortisol levels, measured in pericardial fluid and femoral serum, in relation to the severity of APE, categorized according to specific criteria. Samples were collected from a total of 92 corpses (77 males, 15 females) with a mean age of 56.7 ± 15.2 years. The severity of APE associated with the deaths was classified into three groups: slight or absent (n = 7; 8.6%), medium or moderate (n = 16; 19.8%), and intense (n = 58;71.6%).The determination of MR-proADM and cortisol levels was conducted using ELISA kits and an Immunoassay Analyzer, respectively. Our results reveal a significant increase in MR-proADM concentration with the severity of APE. Furthermore, a correlation was established between cortisol and MR-proADM concentrations in both pericardial fluid and femoral serum samples. This indicates that the severity of APE influences the production of ADM, regardless of the specific underlying pathophysiological mechanisms. Cortisol values were also found to be higher in the intense APE group compared to the moderate group.This study contributes to our understanding of the relationship between MR-proADM and cortisol, and the severity of APE, shedding light on potential applications in postmortem investigations.</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"353-359"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a diagnostic variable number tandem repeat marker and dual TaqMan genotyping assay to distinguish Lophophora species.","authors":"Eun-Mi Hwang, Kyu-Sik Jeong, Seong Yeon Yoo, Jihyun Kim, Sanggil Choe, Joo-Young Kim","doi":"10.1007/s00414-024-03318-9","DOIUrl":"10.1007/s00414-024-03318-9","url":null,"abstract":"<p><p>The Lophophora genus of the Cactaceae family includes Lophophora diffusa and Lophophora williamsii, which has traditionally been used as a natural analgesic; however, its use is now under strict regulation worldwide as it contains mescaline, a unique psychotropic agent. Recently, non-medical and illegal distribution and abuse of L. williamsii have increased worldwide; thus, effective species identification methods are urgently needed. Here, we identified a new variable number tandem repeat (VNTR) marker in the trnL intron region to identify and characterize species in forensic analyses. The VNTR marker has a unique structure of tandem repeats, each with 13 nucleotides; one repeat unit was found in L. williamsii and two in L. diffusa. Phylogenetic and length polymorphism analyses confirmed that this novel VNTR marker could distinguish between Lophophora species. Furthermore, our newly developed TaqMan genotyping assay utilizes two probes; the color and position of dots on the discrimination plot differ according to the tandem repeat count within the VNTR marker. The limits of detection of the assay were 0.000063 ng (LW-VNTR probe-1) and 0.000066 ng (LW-VNTR probe-2), indicating high sensitivity. Moreover, when crime scene samples of 16 presumed L. williamsii species were analyzed, the results coincided with those of gas chromatography-mass spectrometry, confirming the applicability of our marker for Lophophora species identification. Thus, the tandem repeats within the trnL intron region can be exploited as a VNTR marker to identify L. williamsii and L. diffusa. Our dual TaqMan genotyping assay based on a novel marker demonstrates potential for forensic applications.</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"1-13"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142072788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening a new set of microhaplotypes in exonic regions for sample identity testing and paternity testing during whole exome sequencing analysis.","authors":"Yu Tan, Huan Tian, Yuanyuan Xiao, Bocheng Xu, He Wang, Mei Yang, Shanling Liu","doi":"10.1007/s00414-024-03326-9","DOIUrl":"10.1007/s00414-024-03326-9","url":null,"abstract":"<p><p>Whole exome sequencing (WES) is widely used in clinical diagnosis. Before obtaining an accurate diagnosis, it is essential to conduct sample identity testing and paternity testing on trio samples. Currently, there is a lack of optimal genetic markers for these purposes, with limited literature available in this area. Microhaplotypes (MHs) are promising genetic markers due to their high polymorphism, low mutation rate, short amplified fragments, absence of stutter and amplification bias. These characteristics make them suitable for sample tracking and paternity testing during WES analysis. In this study, we screened out a set of polymorphic MHs in exonic regions for the above purposes. The results showed that the power of discrimination (PD) and probability of exclusion (PE) of this set of markers ranged from 0.2682 to 0.8878 and 0.0178 to 0.4583, respectively. Both the cumulative power of discrimination (CPD) and cumulative probability of exclusion (CPE) exceeded 0.999999, indicating the great value of these markers in paternity testing and individual identification in the study population. However, these markers had the effective number of alleles (A_e) values ranging from 1.1784 to 3.8727 (average 2.1805) and informativeness (I_n) values ranging from 0.0151 to 0.2209 (average 0.0766), showing limited value in DNA mixture analysis and biogeographical ancestry inference.</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"77-85"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the binary female/male sex classification: The impact of (trans)gender on the identification of human remains.","authors":"L Küppers, B Gahr, S Ritz","doi":"10.1007/s00414-024-03348-3","DOIUrl":"10.1007/s00414-024-03348-3","url":null,"abstract":"<p><p>In cases of unidentified deceased persons, sex determination is a routine task in forensic medicine. However, the binary biological sex categories 'female' and 'male' may be challenged if it is not clear whether the information in the missing persons databases refers to the biological sex or the (felt and lived) gender. An umbrella term for people who do not identify with their birth sex (which usually is the biological, chromosomal sex) is 'transgender'. In recent decades, the legal and social situation of transgender people has changed in many countries making it easier to live their felt gender more openly. This development highlights the issue of potential challenges in the postmortem identification of transgender individuals. Serious problems in corresponding cases may be rare-but they must be considered and addressed in forensic practice to minimize the risk of delayed or failed identification. The impact of (trans)gender on the identification of human remains was examined by a narrative literature review under special consideration of the prevalences of transgender identities in general populations and in the group of unidentified deceased; possible actions to avoid problems in the postmortem identification of transgender persons in forensic practice are being proposed. One can assume that 1 of 200 people in the United States, the European Union and comparable societies is transgender with an opposite-sex identification, and 2 to 3 of 100 people live outside the typical female/male binary, with numbers increasing. If legally possible, an increasing number of transgender individuals will change their name and gender in civil registration. Transgender individuals are likely to be overrepresented in suicides and in victims of homicides. Although there are no precise data on the prevalence of transgender individuals in the group of unidentified deceased, the remarkably high reported prevalence in the general population and the over-representation of transgender individuals in suicides and homicides suggest that the topic is relevant to forensic practice. An autopsy does not always provide evidence of transgender identity, for example in skeletal remains. Particularly in unsolved cases, the possibility that an unidentified person may have been transgender should be considered. Knowledge and awareness of forensic practitioners on this topic should be strengthened by research and training. Databases and data reporting should be optimized. Recording in antemortem databases should clearly distinguish between 'biological sex' and 'apparent sex /lived gender identity'. When collecting postmortem data, a clear distinction should be made between \"chromosomal sex\" and \"sex based on morphological findings\". CLINICAL TRIAL NUMBER: Not applicable (review article).</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"267-274"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}