International journal of immunopharmacology最新文献_第7页

Down-regulation by a new anti-inflammatory compound, FR167653, of differentiation and maturation of human monocytes and bone marrow CD34+ cells to dendritic cells 新的抗炎化合物FR167653下调人单核细胞和骨髓CD34+细胞向树突状细胞的分化和成熟

International journal of immunopharmacology Pub Date : 2000-07-01 DOI: 10.1016/S0192-0561(00)00014-X

Naoki Nishimura, Yasuhiko Nishioka, Tsutomu Shinohara, Kenji Tani, Saburo Sone

{"title":"Down-regulation by a new anti-inflammatory compound, FR167653, of differentiation and maturation of human monocytes and bone marrow CD34+ cells to dendritic cells","authors":"Naoki Nishimura, Yasuhiko Nishioka, Tsutomu Shinohara, Kenji Tani, Saburo Sone","doi":"10.1016/S0192-0561(00)00014-X","DOIUrl":"10.1016/S0192-0561(00)00014-X","url":null,"abstract":"<div>FR167653 (1-[7-(4-fluorophenyl)-1,2,3,4-tetrahydro-8 (4-pyridyl) pyrazoro [5-1-c] [1], [2], [4] triazin-2-yl]-2-phenylethanedion sulfate monohydrate), one of the pyridinyl imidazoles, is an immunosuppressive agent which was developed to inhibit proinflammatory cytokine production. We examined the effect of FR167653 on the differentiation and maturation phases of both human bone marrow-derived dendritic cells (BM-DC) and blood monocyte-derived DC (Mo-DC). DC induced from either BM-DC or Mo-DC progenitors in the presence of FR167653 had lower expression of CD1a, CD83 and CD86 (B7.2). FR167653 also significantly suppressed the ability of Mo-DC to produce both TNF-α and IL-1β in response to LPS stimulation. Mixed lymphocyte reaction (MLR) stimulation was significantly lower in FR167653-treated Mo-DC than in control Mo-DC, although the suppressive effect of FR167653 was much less on BM-DC. These results indicate novel immunosuppressive properties of FR167653, which may be therapeutically useful in controlling chronic immune and/or inflammatory diseases through down-regulation of DC differentiation and maturation.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 7","pages":"Pages 501-514"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00014-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The effects of allopurinol on immune function in normal BALB/c and SCID mice 别嘌呤醇对正常BALB/c和SCID小鼠免疫功能的影响

International journal of immunopharmacology Pub Date : 2000-07-01 DOI: 10.1016/S0192-0561(00)00018-7

C Kato , K Sato , A Wakabayashi , Y Eishi

{"title":"The effects of allopurinol on immune function in normal BALB/c and SCID mice","authors":"C Kato , K Sato , A Wakabayashi , Y Eishi","doi":"10.1016/S0192-0561(00)00018-7","DOIUrl":"10.1016/S0192-0561(00)00018-7","url":null,"abstract":"<div>To clarify the relationship between purine metabolism and immunity, the in vivo immunosuppressive effects of allopurinol (AL), a xanthinoxidase (XO) inhibitor, were studied using normal BALB/c and severe combined immunodeficient (SCID) mice. Following AL administration for 14 weeks (long term), a decreased immune response to ovalbumin (OVA) in the peripheral blood was observed in normal mice, which might not be only due to direct B cell suppression but also due to suppression of helper T cell function. In the SCID mice, there was a markedly late and reduced recovery of surface immunoglobulin (sIg) positive cells, which are markers for mature B lymphocytes, in the peripheral blood after AL administration. The total immunoglobulin G (IgG) titers in the AL treated group were significantly lower than in the control group 6 weeks after stem cell transfer, but increased until there was no difference in the titers between the two groups at week 14. CD4 positive helper T cells and CD8 positive T cells were slow to recover, though these gradually recovered to reach normal levels in the mature stage. These data suggest that the administration of AL may modulate B cell and T cell responses in OVA-immunized antibody formation. Furthermore, this study showed that AL could influence immune functions during the pre-natal and developmental periods and that its effects might differ according to the stages of maturity of the immune cells.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 7","pages":"Pages 547-556"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00018-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Cefodizime enhances phagocytosis and intracellular killing of Staphylococcus aureus but does not influence polymorphonuclear leukocytes response to fMLP stimulation 头孢地辛增强金黄色葡萄球菌的吞噬和细胞内杀伤，但不影响多形核白细胞对fMLP刺激的反应

International journal of immunopharmacology Pub Date : 2000-07-01 DOI: 10.1016/S0192-0561(00)00019-9

P. Bialasiewicz , R. Stolarek , P. Wejner , G. Piasecka , B. Dutkiewicz , J. Mikucki , D. Nowak

引用次数: 4

Experimental evidence pointing to the bidirectional interaction between the immune system and the thyroid axis 实验证据指向免疫系统和甲状腺轴之间的双向相互作用

International journal of immunopharmacology Pub Date : 2000-07-01 DOI: 10.1016/S0192-0561(00)00012-6

A.J. Klecha , A.M. Genaro , A.E. Lysionek , R.A. Caro , A.G. Coluccia , G.A. Cremaschi

{"title":"Experimental evidence pointing to the bidirectional interaction between the immune system and the thyroid axis","authors":"A.J. Klecha , A.M. Genaro , A.E. Lysionek , R.A. Caro , A.G. Coluccia , G.A. Cremaschi","doi":"10.1016/S0192-0561(00)00012-6","DOIUrl":"10.1016/S0192-0561(00)00012-6","url":null,"abstract":"<div>Among the many examples of neuroendocrine–immune system interactions the relationship between the thyroid axis and the immune function has yet to be clearly established. Here we studied the influence of thyroid hormones on the course of an alloimmune response. Murine T3 and T4 levels were found to be increased a few days after the immunization of mice with allogeneic lymphoid cells. Besides in vivo treatment with T4 was shown to increase alloantibody titers during the early stages of alloimmunization and to enforce lymphoid proliferation in vitro in a mixed lymphocyte reaction. Conversely, lowering thyroid hormone seric levels by propylthiouracil treatment, negatively modulates the humoral and cellular alloimmune responses. The evidence here points to the existence of a bidirectional communication between both systems. The possibility that the antigenic challenge would increase the thyroid gland activity thus leading to a positive modulatory action upon the immune response is also discussed.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 7","pages":"Pages 491-500"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00012-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 89

(9-[4-acetyl-3-hydroxy-2-n-propylphenoxy) methyl]-3-(1H-tetrazol-5-yl)-4H-pyrido [1, 2-a] pyrimidin-4-one), AS-35, inhibits leukotriene synthesis (9-[4-乙酰基-3-羟基-2-n-丙基苯氧基)甲基]-3-(1H-tetrazol-5-yl)- 4h -pyrido [1,2 -a]嘧啶-4-one) AS-35抑制白三烯合成

International journal of immunopharmacology Pub Date : 2000-07-01 DOI: 10.1016/S0192-0561(00)00010-2

Yuhei Hamasaki, Masafumi Zaitu, Kosuke Tsuji, Michiko Miyazaki, Rika Hayasaki, Eriko Muro, Shuichi Yamamoto, Ikuko Kobayashi, Muneaki Matsuo, Tomohiro Ichimaru, Sumio Miyazaki

{"title":"(9-[4-acetyl-3-hydroxy-2-n-propylphenoxy) methyl]-3-(1H-tetrazol-5-yl)-4H-pyrido [1, 2-a] pyrimidin-4-one), AS-35, inhibits leukotriene synthesis","authors":"Yuhei Hamasaki, Masafumi Zaitu, Kosuke Tsuji, Michiko Miyazaki, Rika Hayasaki, Eriko Muro, Shuichi Yamamoto, Ikuko Kobayashi, Muneaki Matsuo, Tomohiro Ichimaru, Sumio Miyazaki","doi":"10.1016/S0192-0561(00)00010-2","DOIUrl":"10.1016/S0192-0561(00)00010-2","url":null,"abstract":"<div>AS-35, (9-[4-acetyl-3-hydroxy-2-n-propylphenoxy) methyl]-3-(1H-tetrazol-5-yl)-4H-pyrido[1, 2-a] pyrimidin-4-one), was developed as a leukotriene (LT) receptor antagonist, which also inhibited IgE-mediated release of leukotrienes (LTs). We have investigated the action of AS-35 on the enzyme activities which are involved in the synthesis of LTC4 and LTB4 (LT-synthesizing enzymes); cytosolic phospholipase A2 (cPLA2), 5-lipoxygenase (5-LO), leukotriene (LT)C4 synthase and LTA4 hydrolase. AS-35 dose-dependently inhibited IgE- and A23187-stimulated production of LTC4 by up to 71.5–84.8% and that of LTB4 by 48.3–49.2% at 2.5×10−5 M. The assays for cPLA2−, 5-LO-, LTC4 synthase- and LTA4 hydrolase-activities revealed that the inhibition is attributable to suppression of cPLA2, 5-LO and LTC4 synthase but not LTA4 hydrolase. We have also studied the action of AS-35 on the release of β-hexosaminidase (β-HEX) as a marker of preformed mediators. AS-35 had only weak inhibitory action on the release of β-HEX. The results indicate that anti-allergic action of AS-35 is predominantly attributable to its inhibition of LT synthesis by suppressing three consecutive enzymes for LTC4 synthesis.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 7","pages":"Pages 483-490"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00010-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Allergen-induced bronchial eosinophilia in guinea-pigs is inhibited by both pre- and post-induction cyclosporin-A treatments 豚鼠过敏原诱导的支气管嗜酸性粒细胞增多症可被诱导前和诱导后的环孢素a治疗所抑制

International journal of immunopharmacology Pub Date : 2000-07-01 DOI: 10.1016/S0192-0561(00)00015-1

Charles I Ezeamuzie , Reginald N.P Nwankwoala

{"title":"Allergen-induced bronchial eosinophilia in guinea-pigs is inhibited by both pre- and post-induction cyclosporin-A treatments","authors":"Charles I Ezeamuzie , Reginald N.P Nwankwoala","doi":"10.1016/S0192-0561(00)00015-1","DOIUrl":"10.1016/S0192-0561(00)00015-1","url":null,"abstract":"<div>Repeated treatment of sensitized guinea-pigs with cyclosporin-A (CS-A) before aerosol allergen challenge is known to inhibit the subsequent bronchial eosinophilia. It is not known, however, if the drug is also effective on established/on-going bronchial eosinophilia. We have, therefore, studied the effect of CS-A on allergen-induced eosinophil recruitment into the bronchoalveolar lavage (BAL) fluid of guinea-pigs when given before or after induction.Ovalbumin-immunized guinea-pigs were treated with CS-A (20 mg/kg subcutaneously) or vehicle daily for varying periods before a single aerosol allergen challenge. In animals in which bronchial eosinophilia was maintained with repeated aerosol allergen challenge, CS-A or vehicle was given daily for varying periods after the first allergen challenge. BAL and cell count were performed 24 h after the last challenge.In vehicle-treated animals, a single allergen challenge caused a 4–5 fold increase in the number of eosinophils in the BAL fluid after 24 h, declining to baseline by 7 days. In repeatedly-challenged animals, this response was sustained throughout. Eosinophil infiltration was significantly inhibited when CS-A was given daily for 7–14 days, but not for 1 or 3 days, before allergen challenge. When given during an established/on-going eosinophil infiltration, a significant inhibition was seen after administration for 5 or 7 days, but not for 1 or 3 days.These results show that repeated CS-A administration inhibits not only the induction of allergic bronchial eosinophilia but also the maintenance of an established one. This may be relevant in the treatment of allergic diseases, such as asthma, in which drug administration often begins when eosinophilia is already established.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 7","pages":"Pages 515-522"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00015-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Impaired estrogen priming of progesterone receptors in uterus of MRL/MP-lpr/lpr mice, a model of systemic lupus erythematosus (SLE) 系统性红斑狼疮(SLE)模型MRL/MP-lpr/lpr小鼠子宫孕酮受体雌激素启动受损

International journal of immunopharmacology Pub Date : 2000-07-01 DOI: 10.1016/S0192-0561(00)00017-5

Yacoub Y Dhaher , Kay Chan , Ben D Greenstein , Elizabeth de Fougerolles Nunn , Munther A Khamashta , Graham R.V Hughes

{"title":"Impaired estrogen priming of progesterone receptors in uterus of MRL/MP-lpr/lpr mice, a model of systemic lupus erythematosus (SLE)","authors":"Yacoub Y Dhaher , Kay Chan , Ben D Greenstein , Elizabeth de Fougerolles Nunn , Munther A Khamashta , Graham R.V Hughes","doi":"10.1016/S0192-0561(00)00017-5","DOIUrl":"10.1016/S0192-0561(00)00017-5","url":null,"abstract":"<div>Estrogens exacerbate the autoimmune disease SLE and progesterone is immunoprotective. Estrogens increase synthesis of progesterone receptors (PR) and it is hypothesized that this physiological balance may be impaired in SLE. To test this, cytosolic PR were measured in hypothalamus, thymus and uterus from 6-week-old female ovariectomized BALB/c and MRL/MP-lpr/lpr mice 48 h after s.c. injection of estradiol benzoate (3.2 μg/0.1 ml; OB) in peanut oil or 0.1 ml peanut oil alone. PR were measured using [3H]ORG 2058, which does not bind to corticosteroid-binding globulin (CBG), and bound and free ligand were separated using minicolumns of Sephadex LH20 at 0°C. PR were measured in cytosols from hypothalamus and uterus of oil-treated BALB/c mice, but were undetectable in thymus, whereas receptors were measurable in all three tissues of MRL mice. There was a significantly greater priming effect of OB on PR in uterus of BALB/c mice, but not in hypothalamus, and PR became detectable in thymus cytosols from BALB/c mice. Also, the apparent affinity of the binding reaction between [3H]ORG 2058 and PR was significantly higher than those measured in other tissues in hypothalamic cytosols of both strains. These results suggest that there is an impairment of estrogen priming of progesterone receptors in uterus and perhaps thymus of MRL mice.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 7","pages":"Pages 537-545"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00017-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Human chorionic gonadotropin induces nitric oxide synthesis by murine microglia 人绒毛膜促性腺激素诱导小鼠小胶质细胞合成一氧化氮

International journal of immunopharmacology Pub Date : 2000-06-01 DOI: 10.1016/S0192-0561(00)00011-4

Hyung-Min Kim , Hong-Kun Rim , Taekyun Shin , Jeong Joong Kim , Seung Taeck Park , Jae Min Oh , Min Kyu Choi , Yeun Tai Chung , Hee Sub Rhee , Jae Yeal Jeung , Ki Nam Lee , Nam Song Kim , Cheorl-Ho Kim

{"title":"Human chorionic gonadotropin induces nitric oxide synthesis by murine microglia","authors":"Hyung-Min Kim , Hong-Kun Rim , Taekyun Shin , Jeong Joong Kim , Seung Taeck Park , Jae Min Oh , Min Kyu Choi , Yeun Tai Chung , Hee Sub Rhee , Jae Yeal Jeung , Ki Nam Lee , Nam Song Kim , Cheorl-Ho Kim","doi":"10.1016/S0192-0561(00)00011-4","DOIUrl":"10.1016/S0192-0561(00)00011-4","url":null,"abstract":"<div>This study investigated the effects of human chorionic gonadotropin (hCG) on the synthesis of nitric oxide (NO) in murine neonatal microglial cells. When hCG was used in combination with interferon-γ (IFN-γ), there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as an increased amount of iNOS protein. The increase of NO synthesis by IFN-γ-plus-hCG was associated with the increase of tumor necrosis factor-α (TNF-α) secretion and hCG-induced NO production was decreased by the treatment with anti-murine TNF-α neutralizing antibody. This study provides evidence that hCG activates expression of iNOS protein in murine microglial cells accompanied by NO accumulation via pathway dependent on l-arginine in the culture medium, and further offers that TNF-α acts on the NO synthesis from IFN-γ-primed murine microglial cells.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 6","pages":"Pages 453-461"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00011-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21579040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Differential impact of nicotine on cellular proliferation and cytokine production by LPS-stimulated murine splenocytes 尼古丁对lps刺激小鼠脾细胞增殖和细胞因子产生的差异影响

International journal of immunopharmacology Pub Date : 2000-06-01 DOI: 10.1016/S0192-0561(00)00005-9

Amal Hakki, Nora Hallquist, Herman Friedman, Susan Pross

{"title":"Differential impact of nicotine on cellular proliferation and cytokine production by LPS-stimulated murine splenocytes","authors":"Amal Hakki, Nora Hallquist, Herman Friedman, Susan Pross","doi":"10.1016/S0192-0561(00)00005-9","DOIUrl":"10.1016/S0192-0561(00)00005-9","url":null,"abstract":"<div>The immunoregulatory effects of nicotine have not been fully clarified and the reported data are often conflicting. The present study investigated the role of nicotine as an immunomodulator of murine splenocytes stimulated by lipopolysaccharide (LPS), the endotoxin component of gram-negative bacteria. BALB/c female mice of two different ages, young (2–3 months) and old (18–22 months), were used. The cells were incubated with nicotine at two different time points, 3 h pre-incubation and concurrent incubation relevant to LPS stimulation, before further incubation for 48 or 72 h. Treatment of murine splenocytes with nicotine showed an impact on cellular proliferation as well as on the production of the pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6). The results indicated that nicotine significantly inhibited cellular proliferation of murine splenocytes in a concentration-related manner (32, 64 and 128 μg/ml). Timing of nicotine exposure prior to LPS stimulation was critical in terms of immunological impact on cytokine production. TNF-α and IL-6 production were significantly enhanced by 1 μg/ml of nicotine when cells were pre-incubated with nicotine for 3 h compared to concurrent incubation relative to LPS stimulation. The alteration in cytokine production varied with the age of the mouse. TNF-α production was significantly inhibited by nicotine in young mice, while IL-6 production was significantly inhibited by nicotine in old mice. Since any immunomodulation that alters the profile of these cytokines may cause an imbalance in the immune system impinging on health status, these findings may be important when dealing with the concept of nicotine as a therapeutic agent.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 6","pages":"Pages 403-410"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00005-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21579035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Inhibitory effect of anaphylactic shock by caffeine in rats 咖啡因对大鼠过敏性休克的抑制作用

International journal of immunopharmacology Pub Date : 2000-06-01 DOI: 10.1016/S0192-0561(00)00006-0

Hye-Young Shin , Chul-Seung Lee , Han-Jung Chae , Hyung-Ryong Kim , Seung-Hwa Baek , Nyeon-Hyoung An , Hyung-Min Kim

{"title":"Inhibitory effect of anaphylactic shock by caffeine in rats","authors":"Hye-Young Shin , Chul-Seung Lee , Han-Jung Chae , Hyung-Ryong Kim , Seung-Hwa Baek , Nyeon-Hyoung An , Hyung-Min Kim","doi":"10.1016/S0192-0561(00)00006-0","DOIUrl":"10.1016/S0192-0561(00)00006-0","url":null,"abstract":"<div>Caffeine is known to reduce evoked histamine secretion, but the effects of caffeine on anaphylactic shock have not been clarified. We have investigated the effects of caffeine on anaphylactic shock in rats. Systemic anaphylactic shock by compound 48/80 injection was monitored for 1 h. An IgE-dependent local anaphylactic shock was generated by sensitizing the skin with anti-dinitrophenyl (DNP) IgE followed 48 h later with an injection of antigen. Caffeine inhibited compound 48/80-induced anaphylatic shock to 40% with a dose of 1 mg/kg. Caffeine (0.1 mg/kg) inhibited to 56.4±0.4% passive cutaneous anaphylactic shock activated by anti-DNP IgE. Caffeine (5–20 mM) significantly inhibited histamine release from rat peritoneal mast cells (RPMCs) activated by compound 48/80 or anti-DNP IgE. Especially, caffeine (20 mM) inhibited by 96.7±0.5% histamine release activated by compound 48/80. Moreover, caffeine (1–20 mM) had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-α production from RPMCs. The level of cAMP in RPMCs, when caffeine (20 mM) was added, increased significantly after 5–60 min compared with that of a normal control. These results indicate that caffeine inhibits immediate-type allergic reactions by inhibition of mast cell degranulation in vivo and in vitro.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 6","pages":"Pages 411-418"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00006-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21579036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16