人绒毛膜促性腺激素诱导小鼠小胶质细胞合成一氧化氮

Hyung-Min Kim , Hong-Kun Rim , Taekyun Shin , Jeong Joong Kim , Seung Taeck Park , Jae Min Oh , Min Kyu Choi , Yeun Tai Chung , Hee Sub Rhee , Jae Yeal Jeung , Ki Nam Lee , Nam Song Kim , Cheorl-Ho Kim
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引用次数: 7

摘要

研究了人绒毛膜促性腺激素(hCG)对小鼠新生小胶质细胞一氧化氮(NO)合成的影响。当hCG与干扰素-γ (IFN-γ)联合使用时,以剂量依赖的方式显著协同诱导NO合成。NO合成的增加表现为iNOS蛋白的增加。IFN-γ- + hcg对NO合成的增加与肿瘤坏死因子-α (TNF-α)分泌的增加有关,抗小鼠TNF-α中和抗体处理可降低hcg诱导的NO生成。本研究证明了hCG在小鼠小胶质细胞中通过l-精氨酸依赖的途径激活iNOS蛋白的表达,并伴随NO的积累,进一步证明了TNF-α对IFN-γ引发的小鼠小胶质细胞中NO的合成起作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human chorionic gonadotropin induces nitric oxide synthesis by murine microglia

This study investigated the effects of human chorionic gonadotropin (hCG) on the synthesis of nitric oxide (NO) in murine neonatal microglial cells. When hCG was used in combination with interferon-γ (IFN-γ), there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as an increased amount of iNOS protein. The increase of NO synthesis by IFN-γ-plus-hCG was associated with the increase of tumor necrosis factor-α (TNF-α) secretion and hCG-induced NO production was decreased by the treatment with anti-murine TNF-α neutralizing antibody. This study provides evidence that hCG activates expression of iNOS protein in murine microglial cells accompanied by NO accumulation via pathway dependent on l-arginine in the culture medium, and further offers that TNF-α acts on the NO synthesis from IFN-γ-primed murine microglial cells.

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