Hyung-Min Kim , Hong-Kun Rim , Taekyun Shin , Jeong Joong Kim , Seung Taeck Park , Jae Min Oh , Min Kyu Choi , Yeun Tai Chung , Hee Sub Rhee , Jae Yeal Jeung , Ki Nam Lee , Nam Song Kim , Cheorl-Ho Kim
{"title":"人绒毛膜促性腺激素诱导小鼠小胶质细胞合成一氧化氮","authors":"Hyung-Min Kim , Hong-Kun Rim , Taekyun Shin , Jeong Joong Kim , Seung Taeck Park , Jae Min Oh , Min Kyu Choi , Yeun Tai Chung , Hee Sub Rhee , Jae Yeal Jeung , Ki Nam Lee , Nam Song Kim , Cheorl-Ho Kim","doi":"10.1016/S0192-0561(00)00011-4","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>This study investigated the effects of human chorionic gonadotropin (hCG) on the synthesis of </span>nitric oxide<span><span> (NO) in murine neonatal microglial cells. When hCG was used in combination with interferon-γ (IFN-γ), there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as an increased amount of iNOS protein. The increase of NO synthesis by IFN-γ-plus-hCG was associated with the increase of tumor necrosis factor-α (TNF-α) secretion and hCG-induced NO production was decreased by the treatment with anti-murine TNF-α </span>neutralizing antibody. This study provides evidence that hCG activates expression of iNOS protein in murine microglial cells accompanied by NO accumulation via pathway dependent on </span></span><span>l</span>-arginine in the culture medium, and further offers that TNF-α acts on the NO synthesis from IFN-γ-primed murine microglial cells.</p></div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 6","pages":"Pages 453-461"},"PeriodicalIF":0.0000,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00011-4","citationCount":"7","resultStr":"{\"title\":\"Human chorionic gonadotropin induces nitric oxide synthesis by murine microglia\",\"authors\":\"Hyung-Min Kim , Hong-Kun Rim , Taekyun Shin , Jeong Joong Kim , Seung Taeck Park , Jae Min Oh , Min Kyu Choi , Yeun Tai Chung , Hee Sub Rhee , Jae Yeal Jeung , Ki Nam Lee , Nam Song Kim , Cheorl-Ho Kim\",\"doi\":\"10.1016/S0192-0561(00)00011-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>This study investigated the effects of human chorionic gonadotropin (hCG) on the synthesis of </span>nitric oxide<span><span> (NO) in murine neonatal microglial cells. When hCG was used in combination with interferon-γ (IFN-γ), there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as an increased amount of iNOS protein. The increase of NO synthesis by IFN-γ-plus-hCG was associated with the increase of tumor necrosis factor-α (TNF-α) secretion and hCG-induced NO production was decreased by the treatment with anti-murine TNF-α </span>neutralizing antibody. This study provides evidence that hCG activates expression of iNOS protein in murine microglial cells accompanied by NO accumulation via pathway dependent on </span></span><span>l</span>-arginine in the culture medium, and further offers that TNF-α acts on the NO synthesis from IFN-γ-primed murine microglial cells.</p></div>\",\"PeriodicalId\":14002,\"journal\":{\"name\":\"International journal of immunopharmacology\",\"volume\":\"22 6\",\"pages\":\"Pages 453-461\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00011-4\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of immunopharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0192056100000114\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of immunopharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0192056100000114","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Human chorionic gonadotropin induces nitric oxide synthesis by murine microglia
This study investigated the effects of human chorionic gonadotropin (hCG) on the synthesis of nitric oxide (NO) in murine neonatal microglial cells. When hCG was used in combination with interferon-γ (IFN-γ), there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as an increased amount of iNOS protein. The increase of NO synthesis by IFN-γ-plus-hCG was associated with the increase of tumor necrosis factor-α (TNF-α) secretion and hCG-induced NO production was decreased by the treatment with anti-murine TNF-α neutralizing antibody. This study provides evidence that hCG activates expression of iNOS protein in murine microglial cells accompanied by NO accumulation via pathway dependent on l-arginine in the culture medium, and further offers that TNF-α acts on the NO synthesis from IFN-γ-primed murine microglial cells.