International journal of immunopharmacology最新文献_第8页

Differential regulation of type IV collagenases and metalloelastase in murine macrophages by the synthetic bacterial lipopeptide JBT 3002 合成细菌脂肽JBT 3002对小鼠巨噬细胞IV型胶原酶和金属弹性酶的差异调节

International journal of immunopharmacology Pub Date : 2000-06-01 DOI: 10.1016/S0192-0561(00)00008-4

Rakesh Kumar, Keping Xie, Ines Eue, Zhongyun Dong, Jerald J. Killion, Isaiah J. Fidler

{"title":"Differential regulation of type IV collagenases and metalloelastase in murine macrophages by the synthetic bacterial lipopeptide JBT 3002","authors":"Rakesh Kumar, Keping Xie, Ines Eue, Zhongyun Dong, Jerald J. Killion, Isaiah J. Fidler","doi":"10.1016/S0192-0561(00)00008-4","DOIUrl":"10.1016/S0192-0561(00)00008-4","url":null,"abstract":"<div>We determined whether the expression of matrix metalloproteinases (MMP) and tissue inhibitors of MMPs (TIMP) in murine macrophages is regulated by the novel synthetic bacterial lipopeptide JBT 3002. Multilamellar liposomes (MLV) encapsulating JBT 3002 (MLV-JBT 3002) stimulated the production of 72-kDa and 92-kDa (gelatinase A and B) type IV collagenase and inhibited the production of murine metalloelastase (MME) in a dose-dependent manner in murine peritoneal macrophages. MLV-JBT 3002 also induced production of TIMP-1. MLV-JBT 3002 did not induce collagenase production in tumor cells. Priming murine macrophages with interferon-gamma (IFN-γ) inhibited JBT 3002-stimulated production of both MMP-9 and MMP-2 and further inhibited production of MME by a mechanism involving nitric oxide (NO). This conclusion is based on data showing that IFN-γ failed to inhibit production of MMP in the presence of l-methyl arginine or in macrophages from inducible nitric oxide synthase knockout mice. These data suggest that JBT 3002 differentially regulates the production of various MMPs and TIMP in macrophages.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 6","pages":"Pages 431-443"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00008-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21579038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Suppression of tumor necrosis factor secretion from white blood cells by synthetic antisense phosphorothioate oligodeoxynucleotides 合成反义硫代寡脱氧核苷酸抑制白细胞肿瘤坏死因子分泌

International journal of immunopharmacology Pub Date : 2000-06-01 DOI: 10.1016/S0192-0561(00)00009-6

Rey-Heng Hu, Shu-Hsun Chu

{"title":"Suppression of tumor necrosis factor secretion from white blood cells by synthetic antisense phosphorothioate oligodeoxynucleotides","authors":"Rey-Heng Hu, Shu-Hsun Chu","doi":"10.1016/S0192-0561(00)00009-6","DOIUrl":"10.1016/S0192-0561(00)00009-6","url":null,"abstract":"<div>In this ex vivo, rather than in vitro, experiment, a synthetic antisense oligodeoxynucleotide was tested to suppress tumor necrosis factor — α(TNF) secretion from lipopolysaccharide-stimulated white blood cells. Antisense oligomer showed significant and specific suppressive effect to the secretion of TNF at concentrations of 1.0 and 10 μM. At the concentration of 1 μM, there were 68.4 and 63.9% suppression of TNF secretion at 2 and 24 h after resuspension of blood cells. At the concentration of 10 μM, the suppressions were slightly higher than those at 1 μM, which were 71.8 and 76.2%, respectively. A 50%-matched scrambler showed suppressive effect only at 10 μM concentration, and the suppression only occurred at 2 and 24 h after incubation. Sense oligomer showed no suppressive effects at any of the concentrations. The specificity of this oligomer was documented by dose-effect phenomenon, sequence-dependent suppression and absence of effect on the synthesis of another cytokine (interleukin-6). A series of parallel studies was performed and showed that all three oligomers at any concentration tested had no effect on the interleukin-6 secretion after LPS stimulation.In conclusion, properly designed antisense oligodeoxynucleotide can significantly and specifically suppress the secretion of TNF by blood cells in an ex vivo system and it may be a good “information” drug to treat diseases that are caused by over production of TNF.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 6","pages":"Pages 445-452"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00009-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21579039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Modulation of lung local immune responses by oral administration of a herbal medicine Sho-saiko-to 口服中草药shoo -saiko-to对肺局部免疫反应的调节

International journal of immunopharmacology Pub Date : 2000-06-01 DOI: 10.1016/S0192-0561(00)00007-2

Nobuhiro Ohtake , Rie Suzuki , Haruyuki Daikuhara , Youichiro Nakai , Masahiro Yamamoto , Sakae Amagaya , Atsushi Ishige , Hiroshi Sasaki , Yasuhiro Komatsu , Kazunori Fukuda , Seiji Hayashi

{"title":"Modulation of lung local immune responses by oral administration of a herbal medicine Sho-saiko-to","authors":"Nobuhiro Ohtake , Rie Suzuki , Haruyuki Daikuhara , Youichiro Nakai , Masahiro Yamamoto , Sakae Amagaya , Atsushi Ishige , Hiroshi Sasaki , Yasuhiro Komatsu , Kazunori Fukuda , Seiji Hayashi","doi":"10.1016/S0192-0561(00)00007-2","DOIUrl":"10.1016/S0192-0561(00)00007-2","url":null,"abstract":"<div>Sho-saiko-to (SST), a Chinese/Japanese herbal medicine (Kampo medicine) widely used to treat chronic hepatitis in Japan, is known to modulate immune responses, and thus its immunomodulating activity may be responsible for its bi-directional effects on the lungs as therapeutic efficacy in various lung diseases and involvement in development of interstitial pneumonia. We administered SST to BALB/c mice orally and examined the lung tissue levels of pro/anti-inflammatory cytokines, interleukin-1beta (IL-1β), tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), and the effects of SST on acute lung injury induced by instillation of lipopolysaccharide (LPS) or IL-1. Although SST had no effect on lung TNF-α or IL-1β level, it increased IL-6. Investigation of active fractions of SST suggested that multiple ingredients were supposed to be responsible for IL-6-inducing activity. Liquiritigenin, a metabolite of liquiritin which is one of the major ingredients in SST enhanced in vitro IL-6 production in anti-CD3 monoclonal antibody (anti-CD3 mAb)-stimulated lung mononuclear cells in a cell-type specific and dose-dependent manner. SST suppressed LPS-induced lung injury at the later phase when lung leak was evident while being ineffective on initial neutrophil sequestration to the lung in these models. These findings suggest that SST modulates lung inflammation by regulating local immune response.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 6","pages":"Pages 419-430"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00007-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21579037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Inhibition of interleukin 5 production with no influence on interleukin 4 production by an anti-allergic drug, tranilast, in Toxocara canis-infected mice 抗过敏药物曲尼司特对犬弓形虫感染小鼠白细胞介素5产生的抑制作用，但对白细胞介素4的产生无影响

International journal of immunopharmacology Pub Date : 2000-06-01 DOI: 10.1016/S0192-0561(00)00013-8

Masahiro Hiratochi , Masaya Takamoto , Satoshi Tatemichi , Kazuo Sugane

{"title":"Inhibition of interleukin 5 production with no influence on interleukin 4 production by an anti-allergic drug, tranilast, in Toxocara canis-infected mice","authors":"Masahiro Hiratochi , Masaya Takamoto , Satoshi Tatemichi , Kazuo Sugane","doi":"10.1016/S0192-0561(00)00013-8","DOIUrl":"10.1016/S0192-0561(00)00013-8","url":null,"abstract":"<div>Tranilast is well-known as a useful drug for allergic diseases. This drug is believed to exhibit its therapeutic effects by inhibiting the release of chemical mediators from mast cells and basophils. Effects of tranilast on T helper type 2 (Th2) cytokine production were investigated in mice infected with Toxocara canis (Tc). Tranilast reduced interleukin (IL)-5 production in a dose-dependent manner but not IL-4 production at all in lung and spleen cells from Tc-infected mice cultured under stimulation with excretory–secretory antigen. Obvious IL-5 mRNA expression was observed at week 1 in the lung alone, and IL-4 mRNA expression was detected at similar levels at weeks 1–6 of infection in both lung and spleen. IL-5 but not IL-4 mRNA expression in the lung was significantly inhibited by daily administration of 100 mg/kg of tranilast for 1 week. This treatment also reduced the serum IL-5 level. Thus, tranilast inhibited IL-5 but not IL-4 production either in vitro or in vivo. The results imply that IL-5 and IL-4 production by Th2 cells may be controlled through different mechanisms.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 6","pages":"Pages 463-471"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00013-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21579041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Immunopharmacological and immunotoxicological activities of a water-soluble (1→3)-β-d-glucan, CSBG from Candida spp. 念珠菌水溶性(1→3)-β-d-葡聚糖CSBG的免疫药理和免疫毒理学活性

International journal of immunopharmacology Pub Date : 2000-05-01 DOI: 10.1016/S0192-0561(99)00093-4

Kazuhiro Tokunaka , Naohito Ohno , Yoshiyuki Adachi , Shigenori Tanaka , Hiroshi Tamura , Toshiro Yadomae

{"title":"Immunopharmacological and immunotoxicological activities of a water-soluble (1→3)-β-d-glucan, CSBG from Candida spp.","authors":"Kazuhiro Tokunaka , Naohito Ohno , Yoshiyuki Adachi , Shigenori Tanaka , Hiroshi Tamura , Toshiro Yadomae","doi":"10.1016/S0192-0561(99)00093-4","DOIUrl":"10.1016/S0192-0561(99)00093-4","url":null,"abstract":"<div>We have established a convenient, two-step procedure to solubilize the yeast cell wall (1→3)-β-d-glucan using the combination of NaClO oxidation and DMSO extraction. Candida soluble β-d-glucan (CSBG) was mainly composed of a linear β-1,3 glucan with a linear β-1,6-glucan moiety. In this study, we screened for several immunopharmacological activities of CSBG and found the following activities: (1) interleukin-6 synthesis of macrophages in vitro; (2) antagonistic effect for zymosan mediated-tumor necrosis factor synthesis of macrophages; (3) augmentation for lipopolysaccharide mediated tumor necrosis factor and nitrogen oxide syntheses of macrophages; (4) activation of alternative pathway of complement; (5) hematopoietic response on cyclophosphamide induced leukopenia; (6) the antitumor effect on ascites form tumor; (7) Enhanced vascular permeability; (8) priming effect on lipopolysaccharide triggered TNF-α synthesis; and (9) adjuvant effect on antibody production. These results strongly suggested that CSBG possessed various immunopharmacological activity.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 5","pages":"Pages 383-394"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(99)00093-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21561217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 107

Generation of antibodies directed against the low-immunogenic peptide-toxins microcystin-LR/RR and nodularin 产生针对低免疫原性肽毒素微囊藻毒素lr /RR和结节素的抗体

International journal of immunopharmacology Pub Date : 2000-05-01 DOI: 10.1016/S0192-0561(99)00086-7

W Baier , M Loleit , B Fischer , G Jung , U Neumann , M Weiß , J Weckesser , P Hoffmann , W.G Bessler , K Mittenbühler

{"title":"Generation of antibodies directed against the low-immunogenic peptide-toxins microcystin-LR/RR and nodularin","authors":"W Baier , M Loleit , B Fischer , G Jung , U Neumann , M Weiß , J Weckesser , P Hoffmann , W.G Bessler , K Mittenbühler","doi":"10.1016/S0192-0561(99)00086-7","DOIUrl":"10.1016/S0192-0561(99)00086-7","url":null,"abstract":"<div>The preparation of antibodies against the liver toxin microcystin, as described here, is of major importance for its detection and purification in food and water, and for a therapeutic approach to neutralize the toxin by passive immunization. Microcystin-LR (MLR) and microcystin-RR (MRR) were purified from cyanobacterial cell materials by extraction, Sephadex LH-20-, ODS silica gel-, ionic exchange and RP-HPLC-chromatography. In order to reduce the toxicity for parenteral administration, microcystins were coupled by the carbodiimide method to poly-l-lysine (PLL50.000). Mice and rabbits were immunized with the conjugates in the presence of two lipopeptide immunoadjuvants (P3CSK4 and P3CS-Th). High MLR-specific antibody levels were observed after parenteral coadministration of antigen and lipopeptides, whereas no anti-MLR antibodies were obtained with free microcystin or the microcystin-PLL50.000-conjugate in the absence of lipopeptide. In oral immunization, coadministration of antigen and adjuvants resulted in an accelerated development of anti MLR-specific antibodies and high antibody levels. Using the antisera, we could detect different microcystins and nodularin down to a concentration range of 10–50 ng/ml by a competitive inhibition ELISA; detection of microcystins in crude cell preparations was also possible. Furthermore, microcystins from different sources could be detected and discriminated from cyclic cyanopeptolines.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 5","pages":"Pages 339-353"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(99)00086-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21561256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

In vivo macrophage activation in chickens with Acemannan, a complex carbohydrate extracted from Aloe vera 用从芦荟中提取的复合碳水化合物阿西甘露聚糖激活鸡体内巨噬细胞

International journal of immunopharmacology Pub Date : 2000-05-01 DOI: 10.1016/S0192-0561(99)00091-0

A Djeraba, P Quere

{"title":"In vivo macrophage activation in chickens with Acemannan, a complex carbohydrate extracted from Aloe vera","authors":"A Djeraba, P Quere","doi":"10.1016/S0192-0561(99)00091-0","DOIUrl":"10.1016/S0192-0561(99)00091-0","url":null,"abstract":"<div>Acemannan (ACM 1), a β-(1,4) -acetylated mannan isolated from Aloe vera, can be used as an effective adjuvant in vaccination against some avian viral diseases. Our results demonstrate a quick and lasting in vivo priming effect of ACM 1 on macrophage response after intramuscular inoculation in chickens (500 μg per 2-month-old bird). In response to IFN-γ in vitro, monocytes from ACM 1-treated chickens exhibited a strong enhancement of NO production from 3 to 9 days p.i., but a weaker effect on MHC II cell surface antigen expression on day 3 p.i.. A stimulating effect of ACM 1 treatment was also observed on spontaneous and inducible NO production for splenocytes only on day 3 p.i.. By that time, splenocytes exhibited a strong higher capacity to proliferate in response to the T cell-mitogen PHA. At the same time, the in vivo capacity to produce NO, measured by the (NO−2+NO−3) serum level after intravenous LPS injection, increased greatly from 3 to 9 days p.i.. In conclusion, ACM 1 was able efficiently and durably to increase the activation capacity of macrophages from the systemic immune compartment (in particular from the blood and spleen after an intramuscular injection) in chickens, especially for NO production. These findings provide a better understanding of the adjuvant activity of ACM 1 for viral and tumoral diseases.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 5","pages":"Pages 365-372"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(99)00091-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21561258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 146

Delta9-tetrahydrocannabinol selectively increases aspartyl cathepsin D proteolytic activity and impairs lysozyme processing by macrophages δ 9-四氢大麻酚选择性地增加天冬氨酸组织蛋白酶D的蛋白水解活性，并损害巨噬细胞对溶菌酶的加工

International journal of immunopharmacology Pub Date : 2000-05-01 DOI: 10.1016/S0192-0561(99)00092-2

Marina Matveyeva, Constance B Hartmann, M.Travis Harrison, Guy A Cabral, Kathleen L McCoy

{"title":"Delta9-tetrahydrocannabinol selectively increases aspartyl cathepsin D proteolytic activity and impairs lysozyme processing by macrophages","authors":"Marina Matveyeva, Constance B Hartmann, M.Travis Harrison, Guy A Cabral, Kathleen L McCoy","doi":"10.1016/S0192-0561(99)00092-2","DOIUrl":"10.1016/S0192-0561(99)00092-2","url":null,"abstract":"<div>Delta9-tetrahydrocannabinol (THC) causes an antigen-dependent defect in the ability of macrophages to activate helper T cells, and this drug-induced impairment is mediated through the peripheral CB2 receptor. Various requirements for the processing of the antigen, lysozyme, were examined to determine where along the pathway THC exerts its influence. A THC-exposed macrophage hybridoma inefficiently stimulated interleukin-2 secretion by a helper T cell hybridoma in response to native lysozyme and its reduced form, suggesting that disulfide bond reduction was unaffected. Cell surface expression of major histocompatibility complex class II molecules was normal on THC-exposed macrophages. The drug-exposed macrophages also competently presented a lysozyme peptide to the T cells, indicating that the class II molecules were functional. The proteolytic activity of two thiol cathepsins was unaltered, but aspartyl cathepsin D activity was significantly increased in THC-exposed macrophages. Thus, selective up-regulation of aspartyl cathepsin activity accompanied the deficiency in lysozyme processing and may contribute, at least in part, to the antigen-dependent processing defect in THC-exposed macrophages.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 5","pages":"Pages 373-381"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(99)00092-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21561259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Effect of Salmonella typhi wild type and O-antigen mutants on human natural killer cell activity 伤寒沙门菌野生型和o抗原突变体对人自然杀伤细胞活性的影响

International journal of immunopharmacology Pub Date : 2000-05-01 DOI: 10.1016/S0192-0561(99)00089-2

Javier Puente , Luz Blanco , Margarita Montoya , Dante Miranda , Inés Contreras , Enrique Vinés , Marion E Wolf , Aron D Mosnaim

{"title":"Effect of Salmonella typhi wild type and O-antigen mutants on human natural killer cell activity","authors":"Javier Puente , Luz Blanco , Margarita Montoya , Dante Miranda , Inés Contreras , Enrique Vinés , Marion E Wolf , Aron D Mosnaim","doi":"10.1016/S0192-0561(99)00089-2","DOIUrl":"10.1016/S0192-0561(99)00089-2","url":null,"abstract":"<div>We investigated the effect of glutaraldehyde-fixed Salmonella typhi Ty2 (Vi−) wild-type (World Health Organization’s vaccine strain) and mutant strains MEI028 (rough, O-antigen−) and MEI012 [smooth (O-antigen+) or rough (partially expressed O-antigen) phenotype when grown at 37 or 30°C, respectively] on natural killer (NK) cell activity of peripheral blood mononuclear cell (PBMC) samples and highly purified (HP; >95%), immunomagnetically isolated NK cell preparations. Incubation of PBMC with each and every one of the S. typhi strains studied consistently and significantly, increased this cellular immune function, as well as the supernatant level of the various cytokines tested e.g. IFN-γ, TNF-α, IL-10 and IL-12 (ELISA). In similar experiments, a significant increase in the cytolytic activity of HPNK cells was elicited by S. typhi Ty2 but not by mutant strain MEI028; neither of the cytokines assayed (IFN-γ and TNF-α) was detected in the supernatant.Our results suggest that S. typhi O-antigen plays an essential role in a mechanism resulting in the direct activation of NK cell activity in HPNK cell preparations. However, the relative quantitative significance of this antigen in the direct stimulation of NK cell cytotoxicity expression in PBMC samples is less clear, as it appears that in this case bacterial-induced monocyte-released cytokines plays a most important role. Incubation with S. typhi Ty2 or MEI028 elicited significant expression of CD69, an early marker of NK cell activation, in PBMC but not in HPNK cell samples (flow cytometry); in similar experiments, the expression of CD16/56 and activation marker CD25 remained essentially unchanged.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 5","pages":"Pages 355-364"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(99)00089-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21561257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effect of adrenalectomy on ethanol-associated changes in lymphocyte cell numbers and subpopulations in thymus, spleen, and gut-associated lymphoid tissues 肾上腺切除术对胸腺、脾脏和肠道相关淋巴组织中淋巴细胞数量和亚群的乙醇相关变化的影响

International journal of immunopharmacology Pub Date : 2000-04-01 DOI: 10.1016/S0192-0561(99)00083-1

Eric L. Padgett , Don A. Sibley , Thomas R. Jerrells

{"title":"Effect of adrenalectomy on ethanol-associated changes in lymphocyte cell numbers and subpopulations in thymus, spleen, and gut-associated lymphoid tissues","authors":"Eric L. Padgett , Don A. Sibley , Thomas R. Jerrells","doi":"10.1016/S0192-0561(99)00083-1","DOIUrl":"10.1016/S0192-0561(99)00083-1","url":null,"abstract":"<div>Consumption of ethanol (ETOH) by experimental animals and human beings is associated with elevated serum levels of corticosteroids. One of the most robust findings associated with ETOH consumption is a loss of lymphocytes from thymus and spleen, as well as from peripheral lymphoid organs to include mesenteric lymph nodes and Peyer’s patches, which are lymphoid organs associated with the gastrointestinal tract. To study the role of corticosteroids in loss of cells from thymus, spleen, and gut-associated lymphoid organs, adrenalectomized (ADX) or intact C57Bl/6 mice were fed a liquid diet containing ETOH (to supply 36% of calories as ETOH) or an isocaloric control diet with a pair-feeding protocol. Loss of lymphocytes from all lymphoid organs was associated closely with serum corticosterone levels in both ETOH-fed and pair-fed groups. ETOH-fed ADX animals showed much less cell loss than did ETOH-fed intact animals. However, there was still an association between ETOH consumption and cell loss when cell loss in ETOH-fed ADX animals was compared with that in ADX pair-fed and ADX chow-fed groups. In both intact and ADX animals ETOH consumption was associated with a loss of immature (CD4+ and CD8+) cells from the thymus. These data lead to the suggestion that corticosteroids are responsible for most of the cell loss from thymus, spleen, mesenteric lymph nodes, and Peyer’s patches in association with ETOH consumption. Some cell loss, however, is independent of corticosteroids. The data presented here also support the suggestion that cell loss from lymphoid organs could be the result of nutritional factors.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 4","pages":"Pages 285-298"},"PeriodicalIF":0.0,"publicationDate":"2000-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(99)00083-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21543509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39