International journal of immunopharmacology最新文献_第6页

Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection 黑籽油对小鼠巨细胞病毒感染的保护作用

International journal of immunopharmacology Pub Date : 2000-09-01 DOI: 10.1016/S0192-0561(00)00036-9

Mohamed Labib Salem , Mohammad Sohrab Hossain

{"title":"Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection","authors":"Mohamed Labib Salem , Mohammad Sohrab Hossain","doi":"10.1016/S0192-0561(00)00036-9","DOIUrl":"10.1016/S0192-0561(00)00036-9","url":null,"abstract":"<div>In this study, antiviral effect of black seed oil (BSO) from Nigella sativa was investigated using murine cytomegalovirus (MCMV) as a model. The viral load and innate immunity mediated by NK cells and Mφ during early stage of the infection were analyzed. Intraperitoneal (i.p.) administration of BSO to BALB/c mice, a susceptible strain of MCMV infection, strikingly inhibited the virus titers in spleen and liver on day 3 of infection with 1×105 PFU MCMV. This effect coincided with an increase in serum level of IFN-γ. Although BSO treatment decreased both number and cytolytic function of NK cells on day 3 of infection, it increased numbers of Mφ and CD4+ T cells. On day 10 of infection, the virus titer was undetectable in spleen and liver of BSO-treated mice, while it was detectable in control mice. Although spleen of both control and BSO-treated mice showed similar CTL activities on day 10 after infection, serum level of IFN-γ in BSO-treated mice was higher. Furthermore, BSO treatment upregulated suppressor function of Mφ in spleen. These results show that BSO exhibited a striking antiviral effect against MCMV infection which may be mediated by increasing of Mφ number and function, and IFN-γ production.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 9","pages":"Pages 729-740"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00036-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21726580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 296

Positive effects of dialyzable leukocyte extract (DLE) on recovery of mouse haemopoiesis suppressed by ionizing radiation and on proliferation of haemopoietic progenitor cells in vitro 可透析白细胞提取物(DLE)对电离辐射抑制小鼠造血功能恢复和体外造血祖细胞增殖的积极作用

International journal of immunopharmacology Pub Date : 2000-08-01 DOI: 10.1016/S0192-0561(00)00025-4

Antonı́n Vacek , Michal Hofer , Karel Barnet , Karel C̆ech , Josef Pekárek , Helena Schneiderová

{"title":"Positive effects of dialyzable leukocyte extract (DLE) on recovery of mouse haemopoiesis suppressed by ionizing radiation and on proliferation of haemopoietic progenitor cells in vitro","authors":"Antonı́n Vacek , Michal Hofer , Karel Barnet , Karel C̆ech , Josef Pekárek , Helena Schneiderová","doi":"10.1016/S0192-0561(00)00025-4","DOIUrl":"10.1016/S0192-0561(00)00025-4","url":null,"abstract":"<div>Dialyzed leukocyte extract (DLE) (Immodin SEVAC, Czech Republic) was shown to enhance the recovery of the pools of hemopoietic stem cells (CFUs) and of granulocyte–macrophage hemopoietic progenitor cells (GM-CFC) in the bone marrow in vivo, as well as to increase the numbers of leukocytes and thrombocytes in the peripheral blood of mice exposed to a sublethal dose of gamma-rays, with an ensuing increase in the numbers of mice surviving the lethal radiation dose. In experiments performed in vitro, DLE or sera of mice administered with DLE were added to cultures of intact mouse bone marrow cells containing suboptimal concentrations of hemopoietic stimulatory cytokines, namely recombinant mouse interleukin-3 (rmIL-3) or recombinant mouse granulocyte–macrophage colony-stimulating factor (rmGM-CSF); under these experimental conditions, both DLE and sera of mice administered DLE were found to increase the counts of GM-CFC colonies in the cultures. It can be hypothesized on the basis of the findings obtained in vitro that the described co-stimulating activity (CoSA) of DLE may play a role also under in vivo conditions; the enhancement of the recovery of hemopoiesis suppressed by ionizing radiation may be due to a co-operation of the stimulatory effects of DLE with the action of cytokines endogenously produced in irradiated tissues.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 8","pages":"Pages 623-634"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00025-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21822077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

MDP and 5-HT receptors MDP和5-HT受体

International journal of immunopharmacology Pub Date : 2000-08-01 DOI: 10.1016/S0192-0561(00)00021-7

Jan Ševčı́k, Vladimı́r Růžička, Josef Slánský, Karel Mašek

{"title":"MDP and 5-HT receptors","authors":"Jan Ševčı́k, Vladimı́r Růžička, Josef Slánský, Karel Mašek","doi":"10.1016/S0192-0561(00)00021-7","DOIUrl":"10.1016/S0192-0561(00)00021-7","url":null,"abstract":"<div>A possible interaction of immunomodulator muramyl dipeptide (MDP) with 5-HT7 (5-hydroxytryptamine) receptors was investigated. The activation of 5-HT7 receptors relaxes the guinea-pig distal ileum. The whole ileum segments were, therefore, cut and placed into the bath. The preparations were precontracted by substance P and potently relaxed by adding incremental concentrations of 5-carboxamidotryptamine (5-CT) (0.01–3.2 μM), less potently by 5-hydroxytryptamine (5-HT) (1–100 μM). The preparations most sensitive to 5-CT were also relaxed by MDP (1–100 μM). Noncumulative concentration–response curves (CRCs) for 5-HT or 5-CT were established in the absence or presence of 5-HT antagonist metergoline (320 nM). Metergoline inhibited the relaxations and shifted the CRCs to the right. In the preparations most sensitive to the effects of both 5-CT and metergoline, the latter substance also inhibited the effect of the highest concentration (100 μM) in CRCs for MDP. In another type of experiments, CRCs for 5-HT or 5-CT were constructed in the presence of low concentrations of MDP (5–500 nM). The relaxations evoked by either drug remained unchanged. These results suggest that low concentrations of MDP do not interact with activation of 5-HT7 receptors. In higher concentrations MDP acts on this receptor type as a very weak partial agonist.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 8","pages":"Pages 587-595"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00021-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21822073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Deferoxamine reduces tissue injury and lethality in LPS-treated mice 去铁胺减少lps处理小鼠的组织损伤和致死率

International journal of immunopharmacology Pub Date : 2000-08-01 DOI: 10.1016/S0192-0561(00)00026-6

Marisa Vulcano , Roberto P. Meiss , Martı́n A. Isturiz

引用次数: 53

The significance of timing of FTY720 administration on the immunosuppressive effect to prolong rat skin allograft survival FTY720给药时间对延长同种异体皮肤移植存活的免疫抑制作用的意义

International journal of immunopharmacology Pub Date : 2000-08-01 DOI: 10.1016/S0192-0561(00)00022-9

Yoshiki Yanagawa, Yukio Hoshino, Kenji Chiba

{"title":"The significance of timing of FTY720 administration on the immunosuppressive effect to prolong rat skin allograft survival","authors":"Yoshiki Yanagawa, Yukio Hoshino, Kenji Chiba","doi":"10.1016/S0192-0561(00)00022-9","DOIUrl":"10.1016/S0192-0561(00)00022-9","url":null,"abstract":"<div>FTY720, a potent immunosuppressant, dramatically decreases the number of peripheral blood lymphocytes within a few hours after administration. The current study assessed the significance of timing of FTY720 administration on the immunosuppressive effect to prolong rat skin allograft survival (WKAH donor to F344 recipient). The median survival time of allografts was 7 days in the control recipients. FTY720 (1 mg/kg/day) significantly prolonged allograft survival when administered from days 0 and 3, but failed to exert an immunosuppressive effect when administered from day 4. Intragraft T cells, especially CD8+ T cells, were markedly increased in number from day 4 to 6, peaking on day 5 in control recipients. FTY720 markedly decreased the number of intragraft CD8+ T cells on day 5 when administered from days 0 and 3. In recipients administered with FTY720 from day 4, the number of intragraft CD8+ T cells were only partially decreased on day 5. Intragraft CD8+ T-cell number in those recipients on day 5 was almost the same as that in control recipients on day 4. In addition, FTY720 did not affect the increase in frequency of CD25+ cells in the CD8+ T-cell subset in allografts. It is likely that recipients treated with FTY720 from day 4 reject allografts by intragraft immune responses involved in CD8+ T cells which had infiltrated before day 4, similar to control recipients. These findings suggest that FTY720 should be administered before increase in T cell infiltration into grafts to inhibit acute allograft rejection.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 8","pages":"Pages 597-602"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00022-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21822074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Protective role of IL-2 during activation of T cells with bryostatin 1 IL-2在苔藓抑素激活T细胞中的保护作用

International journal of immunopharmacology Pub Date : 2000-08-01 DOI: 10.1016/S0192-0561(00)00027-8

Ferdynand J. Kos , David L. Cornell , Anne B. Lipke , Laura J. Graham , Harry D. Bear

{"title":"Protective role of IL-2 during activation of T cells with bryostatin 1","authors":"Ferdynand J. Kos , David L. Cornell , Anne B. Lipke , Laura J. Graham , Harry D. Bear","doi":"10.1016/S0192-0561(00)00027-8","DOIUrl":"10.1016/S0192-0561(00)00027-8","url":null,"abstract":"<div>Pharmacologic agents such as bryostatin 1 (bryostatin) can regulate cell activation, growth, and differentiation by modulating the activities of protein kinase C isoenzymes. Inhibition of growth of tumor cells and activation of T lymphocytes in vitro are the most recognized consequences of bryostatin treatment. The effect of bryostatin on T cells ranges from induction of apoptotic cell death to T cell activation, expansion, and acquisition of antigen-specific effector functions. Here, we describe the conditions under which these wide ranging effects occur. Mouse mammary tumor 4TO7-IL-2-primed lymph node cells exposed ex vivo to bryostatin upregulated CD25 expression but lost the ability to secrete IL-2. Most of these cells died by apoptosis unless IL-2 was provided for the duration of bryostatin treatment. Analysis of T cell repertoire by screening of T cells for the expression of different Vβ T cell receptor (TCR) families revealed that bryostatin-induced T cell death was unbiased and Vβ-nonspecific. Within particular Vβ clones, only CD25+ T cells survived exposure to bryostatin and IL-2. Treatment of 4TO7 tumor-bearing mice with a single injection of low dose bryostatin followed by multiple low doses of IL-2, but not with bryostatin alone, delayed tumor growth. These results indicate that activation of T cells with bryostatin should be carried out under protection of exogenous IL-2 to ensure survival and expansion of T cells that may exhibit anti-tumor activity.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 8","pages":"Pages 645-652"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00027-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21822079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effects of interferons alpha and gamma on cytokine production and phenotypic pattern of human bronchial epithelial cells 干扰素α和γ对人支气管上皮细胞细胞因子产生和表型模式的影响

International journal of immunopharmacology Pub Date : 2000-08-01 DOI: 10.1016/S0192-0561(00)00020-5

Ilja Střı́ž , Tadashi Mio , Yuichi Adachi , Stefano Carnevali , Debra J. Romberger , Stephen I. Rennard

{"title":"Effects of interferons alpha and gamma on cytokine production and phenotypic pattern of human bronchial epithelial cells","authors":"Ilja Střı́ž , Tadashi Mio , Yuichi Adachi , Stefano Carnevali , Debra J. Romberger , Stephen I. Rennard","doi":"10.1016/S0192-0561(00)00020-5","DOIUrl":"10.1016/S0192-0561(00)00020-5","url":null,"abstract":"<div>Human bronchial epithelial cells are involved in airway immune mechanisms through secretion of cytokines and through cell-cell contacts with immunocompetent cells. The aim of our study was to assess the ability of interferon (IFN) alpha and gamma alone and in combination to modulate human bronchial epithelial cell (HBECs) release of the inflammatory cytokines IL-8 and IL-6 and fibronectin and to induce the surface expression of HLA-DR and ICAM-1 molecules involved in immune interactions with other cells. HBECs spontaneously secreted a limited amount of IL-8, which was significantly increased by IFN gamma. IFN alpha inhibited IFN gamma stimulated IL-8 secretion in a concentration-dependent manner. Further, IFN gamma induced IL-6 and fibronectin secretion, and this was also inhibited by IFN alpha. The expression of HLA-DR antigens was significantly increased by IFN gamma and partially inhibited by co-stimulation with IFN alpha. In contrast, IFN gamma also induced ICAM-1 expression by HBECs but co-stimulation with IFN alpha had no significant effect on the expression of this surface antigen. IFN alpha modulation of HBEC functions does not seem to be restricted to IFN gamma stimulation since either stimulatory or inhibitory effects of INF alpha on IL-8 production have been found in pilot experiments using IL-1 beta, TNF alpha, and TGF beta as stimuli. In summary, IFN-gamma induces a number of responses in HBECs including increased secretion of IL-6, IL-8 and fibronectin and increased expression of HLA-DR and ICAM-1. IFN alpha can inhibit all these except expression of ICAM-1 which is unaffected. IFN alpha can also interact with other inflammatory cytokines, but whether the effects are inhibitory or augmentive depends on the cytokines.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 8","pages":"Pages 573-585"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00020-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21822072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Pharmacokinetics and cytokine production in heroin and morphine-treated mice 海洛因和吗啡处理小鼠的药代动力学和细胞因子的产生

International journal of immunopharmacology Pub Date : 2000-08-01 DOI: 10.1016/S0192-0561(00)00023-0

Roberta Pacifici, Simonetta di Carlo, Antonella Bacosi, Simona Pichini, Piergiorgio Zuccaro

{"title":"Pharmacokinetics and cytokine production in heroin and morphine-treated mice","authors":"Roberta Pacifici, Simonetta di Carlo, Antonella Bacosi, Simona Pichini, Piergiorgio Zuccaro","doi":"10.1016/S0192-0561(00)00023-0","DOIUrl":"10.1016/S0192-0561(00)00023-0","url":null,"abstract":"<div>The parallelism between serum levels of heroin and morphine (M) metabolites and the production of interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-10 (IL-10), tumor necrosis factor α (TNF-α), transforming growth factor-β1 (TGF-β1), and interferon-γ (IFN-γ) from murine splenocyte cultures following s.c. injection with 20 mg/kg heroin or M in C57/BL mice is described. The pharmacokinetic profiles of M and inactive morphine-3-glucuronide (M3G) in morphine-treated mice nearly overlapped those in heroin-treated mice, with the only difference being the presence of 6-monoacetylmorphine (AM) in profiles of the latter group. Heroin and M significantly increased production of IL-1β, IL-2, TNF-α and IFN-γ at 3, 20 and 40 min from treatment, peaking at 20 min, though the effect was very brief. At 24 h production was greatly inhibited, and this depressive effect lasted longer than the stimulatory effect. At 48 h only a partial recovery was observed. Heroin and M also had a highly stimulatory effect on the release of anti-inflammatory cytokines such as TGF-β1 and IL-10, though this effect was observed after 120 min, peaking at 24 h and then somewhat decreasing at 48 h. This study demonstrates that the more rapid and pronounced immune response to heroin treatment was due to the presence of AM. Both heroin and M produced a biphasic effect on cytokine production: the central opioid or non-opioid receptors are involved in exogenous opiod-induced stimulatory effects, whereas peripheral opioid or non-opioid receptors are involved in depressive effects. Deficient or excess expression of these key mediators may predispose the host to aberrant defence mechanisms.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 8","pages":"Pages 603-614"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00023-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21822075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 117

Paclitaxel causes mouse splenic lymphocytes to a state hyporesponsive to lipopolysaccharide stimulation 紫杉醇使小鼠脾淋巴细胞对脂多糖刺激反应低下

International journal of immunopharmacology Pub Date : 2000-08-01 DOI: 10.1016/S0192-0561(00)00024-2

Michael Lee , Sung Su Yea , Young Jin Jeon

{"title":"Paclitaxel causes mouse splenic lymphocytes to a state hyporesponsive to lipopolysaccharide stimulation","authors":"Michael Lee , Sung Su Yea , Young Jin Jeon","doi":"10.1016/S0192-0561(00)00024-2","DOIUrl":"10.1016/S0192-0561(00)00024-2","url":null,"abstract":"<div>Multiple immune system actions have been ascribed to paclitaxel (taxol), a novel anticancer drug, including the capacity to induce macrophage antitumor cytotoxic molecule production. In the present studies, we demonstrated that paclitaxel produced a selective inhibition of lipopolysaccharide (LPS)-induced B cell proliferation. Similarly, in vitro polyclonal antibody-forming cell responses also were found to be inhibited by paclitaxel. Conversely, paclitaxel exhibited no inhibitory effects on concanavalin A (Con A)-induced T cell proliferation. To study the pathway leading to paclitaxel-induced immunosuppression, we analyzed Raf-1/ERK and JNK/p38 MAPK pathways, both of which have been reported to be involved in LPS signaling. Our results indicate that taxol treatment inhibits Raf-1 kinase activation while having no effect on ERK activation suggesting that ERK activation is distinct from upstream Raf-1 kinase in taxol-induced immunomodulation. Furthermore, paclitaxel pretreatment caused down-regulation of stress-activated MAPKs, JNK and p38 MAPK in lipopolysaccharide (LPS)-stimulated mouse splenic lymphocytes, demonstrating that spleen cells are induced to a state hyporesponsive to LPS stimulation by pre-exposing them to paclitaxel. Taken together, these results suggest that down-regulation of JNK/p38 MAP kinase may contribute to paclitaxel-induced immunosuppression in mouse splenic lymphocytes.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 8","pages":"Pages 615-621"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00024-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21822076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Restoration of splenic noradrenergic nerve fibers and immune reactivity in old F344 rats: a comparison between L-deprenyl and L-desmethyldeprenyl 老龄F344大鼠脾去甲肾上腺素能神经纤维的恢复及免疫反应性:l -去甲烯丙烯与l -去甲烯丙烯的比较

International journal of immunopharmacology Pub Date : 2000-07-01 DOI: 10.1016/S0192-0561(00)00016-3

S ThyagaRajan , K.S Madden , S.Y Stevens , D.L Felten

{"title":"Restoration of splenic noradrenergic nerve fibers and immune reactivity in old F344 rats: a comparison between L-deprenyl and L-desmethyldeprenyl","authors":"S ThyagaRajan , K.S Madden , S.Y Stevens , D.L Felten","doi":"10.1016/S0192-0561(00)00016-3","DOIUrl":"10.1016/S0192-0561(00)00016-3","url":null,"abstract":"<div>L-deprenyl, a monoamine oxidase-B inhibitor, partially reversed the age-associated decline in splenic sympathetic noradrenergic (NA) innervation and immune reactivity in old male rats. The purpose of the present study was to examine whether the effects of deprenyl on splenic sympathetic NA nerve fibers and immune functions are mediated through a metabolite of deprenyl, L-desmethyldeprenyl. Old male F344 rats were treated with 0, 0.25, or 1.0 mg L-(−)-deprenyl/kg BW; 0.025, 0.25, or 1.0 mg L-(−)-desmethyldeprenyl/kg BW; and 1.0 mg D-(+)-desmethyldeprenyl/kg BW i.p. daily for 8 weeks. The animals were sacrificed after a 10-day drug wash-out period and the spleens were removed for histofluorescence, immunocytochemistry, neurochemical, and immunological analysis. The volume density of NA nerve fibers was increased in the spleens of deprenyl- and L-desmethyldeprenyl-treated old rats. Con A-induced IFN-γ production by spleen cells was elevated in 1.0 mg/kg deprenyl- and L-desmethyldeprenyl-treated rats in comparison to saline- and D-desmethyldeprenyl-treated old rats. Deprenyl and desmethyldeprenyl treatment did not alter the percentage of CD5+ T cells, but treatment with 1.0 mg/kg deprenyl and 0.025 mg/kg L-desmethyldeprenyl prevented the decline in the percentage of sIgM+B cells in the spleens of old rats. These results suggest that L-desmethyldeprenyl may be as equipotent as deprenyl in preventing age-associated diminution in splenic sympathetic NA innervation and immunocompetence.</div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 7","pages":"Pages 523-536"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00016-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21633694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11