International journal of hygiene and environmental health最新文献

{"title":"Environmental exposure to polychlorinated dibenzo-p-dioxins and dibenzofurans among population living in a contaminated area in Lausanne, Switzerland","authors":"Christelle Oltramare ,&nbsp;David Vernez ,&nbsp;Valentin Rousson ,&nbsp;Markus Zennegg ,&nbsp;Yves Henchoz ,&nbsp;Murielle Bochud ,&nbsp;Aurélie Berthet","doi":"10.1016/j.ijheh.2025.114619","DOIUrl":"10.1016/j.ijheh.2025.114619","url":null,"abstract":"<div><div>Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) are ubiquitous in the environment and the main route of exposure for humans is food. A widespread soil contamination was discovered in 2021 in Lausanne city. Previous human exposure assessment study showed that consumption of home-produced eggs and cucurbits were dominant exposure scenarios. In the absence of recent biomonitoring data on PCDD/Fs in the Swiss general population, we conducted a cross-sectional study in 2023 to assess the difference between control (n = 50) and exposed (n = 51) groups, living in the contaminated area and reporting to consume selected home-produced foods (eggs and cucurbits). We compared the two groups using multivariable modelling. The geometric mean serum PCDD/Fs concentration was 6.1 (Interquartile range (IQR): 4.2) and 6.9 (IQR: 5.0) pg TEQ₀₅/g lipid in the control and exposed groups, respectively. In the exposed group, the serum concentrations were 7.7 (IQR: 4.6) and 6.4 (IQR: 3.8) pg TEQ₀₅/g lipid for those who consumed home-produced eggs or cucurbit, respectively. In unadjusted analysis, control and exposed groups did not significantly differ. However, multivariable analyses showed that older age, female sex, higher body fat mass and consumption of home-produced eggs from contaminated areas were independently and significantly associated with higher PCDD/F concentrations. Health risk for humans living on contaminated areas depends on the land use. These results confirm the importance of dietary factors of animal origin as an important route of PCDD/Fs exposure. Unlike cucurbits, home-produced eggs from contaminated areas represent an important source of exposure to PCDD/Fs, which helps guiding public health recommendations.</div></div>","PeriodicalId":13994,"journal":{"name":"International journal of hygiene and environmental health","volume":"268 ","pages":"Article 114619"},"PeriodicalIF":4.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First application of enterophages for monitoring faecal pollution in the temperate Baltic region of Northern Poland","authors":"Agata Jurczak-Kurek ,&nbsp;Joanna Całkiewicz ,&nbsp;Ewa Kotlarska","doi":"10.1016/j.ijheh.2025.114622","DOIUrl":"10.1016/j.ijheh.2025.114622","url":null,"abstract":"<div><div>This study investigates the abundance of enterophages, a novel faecal indicator, in raw and treated sewage at two Wastewater Treatment Plants (WWTPs) in Pomerania, Poland, serving a population of 1.2 million inhabitants. Using the ISO-standardized double agar layer (DAL) method, we compared enterophages with other biological indicators, including coliphages, <em>Escherichia coli</em>, and enterococci. We observed that, although enterophages were present, they formed smaller plaques than coliphages, which complicated detection. Despite this, the study identified a strong presence of <em>E. coli</em> in both raw and treated wastewater, with lower concentrations of enterophages, consistent with other global studies. Enterophages were found to be less abundant than bacterial indicators and coliphages, suggesting a potentially more specific human source. The concentration of enterophages in treated sewage decreased significantly, supporting their potential as indicators of untreated sewage contamination in water bodies. Our results also revealed that enterophages counts in raw sewage were higher than those reported in tropical regions, possibly due to differences in methodology and bacterial strains used. Furthermore, correlation analyses indicated that microbial indicators in raw sewage were linked with chemical parameters such as temperature and orthophosphates, which may influence their presence. The study concludes that while enterophages are viable alternative faecal indicators, their low abundance in treated wastewater highlights the need for further research, including survivability studies and the development of molecular detection methods. This research contributes to the understanding of enterophages in temperate climates and underscores the importance of regional collaboration in optimizing faecal contamination monitoring methodologies.</div></div>","PeriodicalId":13994,"journal":{"name":"International journal of hygiene and environmental health","volume":"268 ","pages":"Article 114622"},"PeriodicalIF":4.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144605479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardised protocol for the detection and quantification of nontuberculous mycobacteria (NTM) in tap water and its application in investigating the source of NTM clinical infections","authors":"Camille Allam ,&nbsp;Sophie Haenn ,&nbsp;Emma Giraud ,&nbsp;Zeina Awad ,&nbsp;Jérôme Robert ,&nbsp;Emmanuelle Cambau ,&nbsp;Laurent Moulin ,&nbsp;Faïza Mougari","doi":"10.1016/j.ijheh.2025.114618","DOIUrl":"10.1016/j.ijheh.2025.114618","url":null,"abstract":"<div><div>Nontuberculous mycobacteria (NTM) are emerging pathogens causing pulmonary and extrapulmonary diseases, including healthcare-related infections. Although water is one of the main infection sources, isolating NTM from water samples is rarely done due to the NTM fastidious growth and lack of a standardised method. We propose a standardised NTM detection protocol from tap water. We set a protocol for NTM cultivation, measured its repeatability (n = 20 experiments, 5 for each condition), reproducibility (n = 20, 5 for each condition), and performed an inter-laboratory comparison (n = 6) using sterile and tap water samples spiked with slowly growing (<em>M. avium</em>) and rapidly growing (<em>M. chelonae</em>) NTM, plus artificial contamination (<em>Pseudomonas aeruginosa</em>). We investigated water-related NTM infections using this protocol from 2014 to 2024. The protocol showed good repeatability, reproducibility, and recovery yield (range: 67–131 %) for quantifying NTM in artificial samples. The inter-laboratory agreement was 83 %. It was consistent with different growth media and temperatures. The addition of <em>P. aeruginosa</em> did not affect NTM recovery. We used the protocol in 12 investigations, involving 24 patients. NTM were detected in 11/12 investigations (92 %, 23 patients) with species being the same as patients’ isolates in 6/11 (55 %, 18 patients). Water was identified as a source of infection in 5/12 (42 %) investigations involving 15/24 patients (62 %) based on matching genotypes. This study provides a protocol for detecting and quantifying NTM colonies in tap water, which allows identifying the source of clinical infections. With an increase in NTM infections, using this method in mycobacteriology and in environmental laboratories could be beneficial.</div></div>","PeriodicalId":13994,"journal":{"name":"International journal of hygiene and environmental health","volume":"268 ","pages":"Article 114618"},"PeriodicalIF":4.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenol biomarker concentrations in human ovarian follicular fluid and the associations with in-vitro fertilization outcomes","authors":"Irene Dimitriadis ,&nbsp;Irene Souter ,&nbsp;Paige L. Williams ,&nbsp;David Weller ,&nbsp;Jennifer B. Ford ,&nbsp;Russ Hauser ,&nbsp;Lidia Mínguez-Alarcón","doi":"10.1016/j.ijheh.2025.114617","DOIUrl":"10.1016/j.ijheh.2025.114617","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Phenols are a family of short-lived endocrine disrupting chemicals found in a wide range of products and have drawn significant attention because of widespread human exposure and their potential adverse effects on reproductive health. Phenols have been widely detected in several human bodily fluids, particularly in urine and blood. However, there is limited data on phenols in human ovarian follicular fluid (FF). In addition, studies suggest associations between exposure to bisphenols and measures of infertility in humans. Nevertheless, the association of FF concentrations of bisphenols and female fertility has not been investigated.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Objectives&lt;/h3&gt;&lt;div&gt;To quantify phenols in human ovarian FF, investigate correlations of phenol concentrations between FF and urine, evaluate trends over time, and explore any associations between FF concentrations of phenols and &lt;em&gt;in-vitro&lt;/em&gt; fertilization (IVF) outcomes.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;This analysis includes 143 women who enrolled in the Environment and Reproductive Health (EARTH) Study and underwent one IVF cycle between 2009 and 2015, with available, FF, urine and reproductive outcome data. FF concentrations of 2,4-dichloro-phenol (2,4DCP), 2,5-dichloro-phenol (2,5DCP), methyl-paraben (MPB), ethyl-paraben (EPB), propyl-paraben (PPB), butyl-paraben (BPB), bisphenol A (BPA), bisphenol S (BPS), bisphenol F (BPF), benzophenone-3 (BP3), triclosan (TCS) and triclorocarban (TCC) were quantified by isotope dilution tandem mass spectrometry. Generalized linear models were used to explore the associations between FF concentrations of bisphenols (in tertiles or dichotomized) and IVF outcomes, adjusting for confounders.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Detection rates varied from high (79–95 %) for MPB, PPB and BPS, to moderate (44–65 %) for BPA, BP3 and TCS, to low (1–34 %) for 2,4DCP, 2,5DCP, EPB, BPB, BPF, TCC. Correlations between FF and urine were strong for BP3 (&lt;em&gt;r&lt;/em&gt; = 0.86), moderate for MPB (&lt;em&gt;r&lt;/em&gt; = 0.64) and PBP (&lt;em&gt;r&lt;/em&gt; = 0.63), and weak for BPS (&lt;em&gt;r&lt;/em&gt; = 0.21) and BPA (&lt;em&gt;r&lt;/em&gt; = 0.12). FF BPA concentrations significantly decreased over time, whereas FF BPS concentrations increased. Most of the examined FF phenol biomarker concentrations were not related to early IVF outcomes (endometrial thickness, total and mature (MII) oocyte yield, fertilization rates). However, significant associations between PPB and endometrial thickness, and between BP3 and MII oocyte yield were noted, without these findings translating in any effects observed on pregnancy outcomes (implantation, clinical pregnancy and live birth).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;We observed that most phenols were detected in FF and there were some correlations with urinary concentrations. We confirmed in FF that phenols such as BPA may be declining, while exposure to new replacements such as BPS are increasing. However, no overall associations were o","PeriodicalId":13994,"journal":{"name":"International journal of hygiene and environmental health","volume":"268 ","pages":"Article 114617"},"PeriodicalIF":4.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In utero exposure to a mixture of phthalates, parabens, and other phenols and menstrual cycle characteristics in adolescents","authors":"Olivia P. Stoddard ,&nbsp;Kimberly Berger ,&nbsp;Brenda Eskenazi ,&nbsp;Katherine Kogut ,&nbsp;Nina T. Holland ,&nbsp;Stephen Rauch ,&nbsp;Kim G. Harley","doi":"10.1016/j.ijheh.2025.114612","DOIUrl":"10.1016/j.ijheh.2025.114612","url":null,"abstract":"<div><div>Phthalates, parabens, and other phenols are present in consumer products humans use every day, including personal care products and food packaging. Exposure to these chemicals may have endocrine disrupting effects. The menstrual cycle is guided by the rise and fall of hormones, which may be disrupted by exposure to these chemicals. Urinary concentrations of metabolites of phthalates, parabens, and phenols in mothers during pregnancy and several menstrual cycle characteristics in their daughters at age 16 were examined in a predominantly Latino farmworker cohort. The association between the chemicals and each outcome was examined using logistic regression. Bayesian hierarchical modeling was used to model the chemical mixture's associations with each outcome. All models were adjusted for poverty level during pregnancy, maternal pre-pregnancy BMI, and fast-food consumption at adolescent age 9. Results showed a positive association between mono (3-carboxypropyl) phthalate, propylparaben, and bisphenol-A and heavy menstrual flow. Exposure to 2,4-dichlorophenol was positively associated with short cycle length. Mono(3-carboxypropyl) phthalate and di (2-ethylhexyl) phthalate were positively associated with long cycle length. These results suggest <em>in utero</em> exposure to phthalates, parabens, and other phenols may be associated with abnormal menstrual cycle characteristics in adolescents.</div></div>","PeriodicalId":13994,"journal":{"name":"International journal of hygiene and environmental health","volume":"268 ","pages":"Article 114612"},"PeriodicalIF":4.5,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbaryl use and incident cancer in the Agricultural Health Study: an updated analysis","authors":"Patricia A. Erickson ,&nbsp;Gabriella Andreotti ,&nbsp;Richard V. Remigio ,&nbsp;Mary H. Ward ,&nbsp;Christine G. Parks ,&nbsp;Dale P. Sandler ,&nbsp;Paul S. Albert ,&nbsp;Kathryn Hughes Barry ,&nbsp;Jonathan N. Hofmann ,&nbsp;Laura E. Beane Freeman","doi":"10.1016/j.ijheh.2025.114615","DOIUrl":"10.1016/j.ijheh.2025.114615","url":null,"abstract":"<div><div>Carbaryl is a common carbamate insecticide in the United States (USA). Previous epidemiologic investigations, including within the Agricultural Health Study (AHS), have suggested potential associations between carbaryl use and cancer risk. The AHS is a prospective cohort study of licensed pesticide applicators in North Carolina (NC) and Iowa (IA), USA. Information on lifetime pesticide use was reported at enrollment (1993–1997) and follow-up (1999–2005). We evaluated cancer risks associated with ever- and intensity-weighted lifetime days (IWLD) of carbaryl use. Among 52,625 applicators, 8713 incident cancer cases were identified from linkages with state cancer registries through 2014 (NC) or 2017 (IA). We used Poisson regression to estimate rate ratios (RR) and 95 % confidence intervals (CI), controlling for confounders, and evaluated lagged exposures. Approximately 51 % of applicators reported using carbaryl. Increasing IWLD of carbaryl use was associated with increased stomach cancer risk (third tertile vs. never use; RR_T3 = 2.07, 95 % CI: 1.05–4.07<em>, p-trend</em> = 0.02), persisting when exposure was lagged by 5-years (RR_T3 = 2.20, 95 % CI: 1.12–4.33). We noted elevated risks of esophageal (RR = 1.52, 95 % CI: 1.01–2.27) and tongue (RR = 1.91, 95 % CI: 0.95–3.81) cancers with ever-use. There was an increased risk of aggressive prostate cancer when carbaryl exposure was lagged by 30 years (RR_lag30Q4 = 1.56, 95 % CI: 1.18–2.08, <em>p-trend</em> = 0.002). This is the largest and most comprehensive prospective evaluation of carbaryl and cancer risk to date. We provide novel evidence of associations between carbaryl exposure and specific cancers. There is a need for additional studies to confirm these findings and to elucidate the biological mechanisms underlying the observed associations.</div></div>","PeriodicalId":13994,"journal":{"name":"International journal of hygiene and environmental health","volume":"268 ","pages":"Article 114615"},"PeriodicalIF":4.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A physiologically based kinetic model for quantifying human exposure to DEET","authors":"Min-Soo Kim ,&nbsp;Abdullah Hamadeh ,&nbsp;Jason Kowalski ,&nbsp;Amber Yeary ,&nbsp;Puneet Vij ,&nbsp;Jacob Torres ,&nbsp;David Kim ,&nbsp;Andrea N. Edginton","doi":"10.1016/j.ijheh.2025.114616","DOIUrl":"10.1016/j.ijheh.2025.114616","url":null,"abstract":"<div><h3>Background</h3><div>N,N-diethyl-3-methylbenzamide (DEET) is an active ingredient of insect repellents that are applied on skin against mosquitoes and ticks. We developed a human PBK model of DEET and 3-(diethylcarbamoyl)benzoic acid (DCBA, a primary metabolite of DEET). The PBK model was used to interpret a biomonitoring study of DEET in children.</div></div><div><h3>Methods</h3><div>The model was built in three steps. First, systemic models of DEET and DCBA were built and linked based on <em>in vitro</em> and <em>in vivo</em> data and <em>in silico</em> predictions. Second, a mechanistic dermal absorption model of DEET was developed and optimized based on <em>in vitro</em> permeation test (IVPT) data. Finally, the systemic model was linked to the dermal model. Predictions of the integrated model were evaluated using <em>in vivo</em> dermal exposure study data. This model was used to interpret urine concentrations of DEET following use of DEET containing repellents by children at a summer camp.</div></div><div><h3>Results</h3><div>The model (<em>i.e</em>., combined dermal and systemic models) whose urinary predictions were within the 2-fold range was used to simulate the time-course profile of DEET; results were compared against data from a previously published pharmacokinetic study. The estimated typical amount of applied DEET at the summer camp over 24 h was 69.0 mg with a predicted dermal bioavailability of 24.2 %.</div></div><div><h3>Conclusion</h3><div>A PBK model of DEET and DCBA was developed and validated in humans by integrating publicly available data. The external dose of DEET was estimated using the PBK model, and the calculated margin of exposure is above the level of concern.</div></div>","PeriodicalId":13994,"journal":{"name":"International journal of hygiene and environmental health","volume":"268 ","pages":"Article 114616"},"PeriodicalIF":4.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of parental occupational exposures on autism spectrum disorder severity and skills in cognitive and adaptive domains in children with autism spectrum disorder","authors":"Erin C. McCanlies ,&nbsp;Ja Kook Gu ,&nbsp;Claudia C. Ma ,&nbsp;Wayne T. Sanderson ,&nbsp;Yunin J. Ludeña-Rodriguez ,&nbsp;Irva Hertz-Picciotto","doi":"10.1016/j.ijheh.2025.114613","DOIUrl":"10.1016/j.ijheh.2025.114613","url":null,"abstract":"","PeriodicalId":13994,"journal":{"name":"International journal of hygiene and environmental health","volume":"268 ","pages":"Article 114613"},"PeriodicalIF":4.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extensive exposure to di-n-hexyl phthalate with significant seasonal variation across infants, children and adults including pregnant women in Denmark","authors":"Hanne Frederiksen ,&nbsp;Holger M. Koch ,&nbsp;Stine Müller ,&nbsp;Margit Bistrup Fischer ,&nbsp;Gylli Mola ,&nbsp;Casper P. Hagen ,&nbsp;Anders Juul ,&nbsp;Tina Kold Jensen ,&nbsp;Anna-Maria Andersson","doi":"10.1016/j.ijheh.2025.114614","DOIUrl":"10.1016/j.ijheh.2025.114614","url":null,"abstract":"<div><h3>Background</h3><div>Di-n-hexyl phthalate (DnHxP) is one of the most potent phthalates with adverse effects on the male reproductive system. Despite a European ban on DnHxP since 2020, high urinary excretion of mono-n-hexyl phthalate (MnHxP), the major metabolite of DnHxP, has been observed in recent European human biomonitoring (HBM) studies. Sunscreen products containing the UV-filter diethylamino hydroxybenzyl hexyl benzoate (DHHB), which can be contaminated with DnHxP, were pointed out as a relevant source of exposure to DnHxP.</div></div><div><h3>Objective</h3><div>To study possible seasonal variation in urinary excretion of MnHxP in different Danish study populations.</div></div><div><h3>Method</h3><div>MnHxP was measured by LC-MS/MS in 1591 urine samples collected in 2016–2022 from pregnant women, 7-year-old children, and infants and their parents participating in three different cohort studies.</div></div><div><h3>Results</h3><div>Urinary MnHxP was above the limit of detection (0.04 μg/L) in 84 % and above 1 μg/L in 33 % of samples. The 7-year-old children was the study population with the highest excretion rate (97 %). The highest urinary MnHxP concentration (72.5 μg/L) exceeded the health-based guidance value (HBM-I value) of 60 μg/L.</div><div>A significantly higher urinary excretion of MnHxP was observed in the summer compared to the winter season in all study populations. 80 % of the urine samples with MnHxP concentrations &gt; 1 μg/L were from the summer season. 40 % of Danish sunscreen products contain DHHB.</div></div><div><h3>Conclusion</h3><div>The marked seasonal variation with higher MnHxP excretions in the summer season supports the hypothesis that the recently unveiled DnHxP contamination of the UV-filter DHHB in sunscreen products could be driving these exposures.</div></div>","PeriodicalId":13994,"journal":{"name":"International journal of hygiene and environmental health","volume":"268 ","pages":"Article 114614"},"PeriodicalIF":4.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of antimicrobial resistance in Escherichia coli from dairy farm environment","authors":"Namita Narwal ,&nbsp;Monica Rose Amarlapudi ,&nbsp;Ravikant V. Vinchurkar ,&nbsp;Manisha Behera ,&nbsp;Sachinandan De ,&nbsp;Raghu Hirikyathanahalli Vishweswaraiah ,&nbsp;Anil Kumar Puniya ,&nbsp;Rashmi Hogarehalli Mallappa ,&nbsp;Diwas Pradhan","doi":"10.1016/j.ijheh.2025.114607","DOIUrl":"10.1016/j.ijheh.2025.114607","url":null,"abstract":"<div><div>The present study was carried out to study the prevalence and genetic mechanisms of antimicrobial resistance in <em>E. coli</em> strains from dairy farms. A total of 60 <em>E. coli</em> strains were initially isolated from 192 dairy farm samples using a selective antibiotic approach and confirmed as <em>E. coli</em> by PCR. Among these, 48 <em>E. coli</em> isolates predominantly from fecal samples were further studied. These isolates were majorly classified in phylogroup A (43.75 %) and B1 (16.66 %) and showed predominant resistance against ampicillin (60.41 %) followed by piperacillin (56.25 %), tetracycline (54.16 %), and other β-lactams such as cefotaxime (47.91 %) and cefuroxime (43.75 %). A significant portion (22.9 %) of the <em>E. coli</em> isolates were multidrug-resistant (MDR) and 50 % were ESBL-positive. Multiple antibiotic resistance (MAR) index ≥0.4 was exhibited by three isolates. Genotypic analysis identified resistance genes associated with β-lactams (<em>bla</em>_{<em>CTX-M-1</em>}, 64.58 %; <em>bla</em>_<em>TEM</em>, 35.41 %; <em>bla</em>_{<em>CTX-M-9</em>,} 4.16 %), tetracycline (<em>tet</em>A,37.58 %; <em>tet</em>B, 47.91 %), trimethoprim (<em>dfr</em>A17, 16.66 %), aminoglycosides [<em>aac(6′)-Ib-cr</em>, 2.08 %] and fluoroquinolones [<em>qnr</em>B, 25 %; <em>qnr</em>S, 16.66 %; <em>gyr</em>B (S492N), 45.83 %; <em>gyr</em>A (S83L), 45.83 %; <em>gyr</em>A(S87L), 39.58 %; <em>par</em>C (S80I), 14.58 %]. <em>E. coli</em> isolates also showed a high frequency of mobile genetic elements (MGEs) such as IS26 (56.25 %), IncFIB plasmids (52.08 %), and Tn3 transposons (56.25 %). Class 1 integrons harbouring 200 and 800 bp gene cassettes were also detected in 5 <em>E. coli</em> isolates (10.4 %). Overall, this study highlights the high prevalence of diverse AMR genes in cattle-derived <em>E. coli</em> and their strong association with various MGEs. These findings emphasize the need for continuous genomic surveillance to mitigate resistance spread, particularly within and from dairy environments.</div></div>","PeriodicalId":13994,"journal":{"name":"International journal of hygiene and environmental health","volume":"268 ","pages":"Article 114607"},"PeriodicalIF":4.5,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}