International Journal of Clinical Biochemistry and Research最新文献

{"title":"Evaluation of vitamin E levels, antioxidant enzymes and malondialdehyde in women with unexplained infertility","authors":"None Vanitha S, Victoria Job, None Aleyamma T K","doi":"10.18231/j.ijcbr.2023.034","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.034","url":null,"abstract":"Unexplained infertility is a perplexing disorder. 30% of infertile couples are diagnosed with unexplained infertility with all standard fertility investigations normal. Oxidative stress has been shown to have a negative impact on reproductive function in women with endometriosis and (PCOS) polycystic ovarian syndrome. In another study antioxidant supplementation improved OS induced infertility. Many studies have demonstrated an increase in Malondialdehyde in serum of women with infertility and vitamin E as a protective antioxidant in the body with positive effect on the fertility. There is growing evidence linking OS and unexplained infertility. Based on this knowledge the specific group of unexplained infertile women were chosen to assess their levels of serum antioxidant enzymes, Vitamin E and lipid peroxidation marker. To compare the Levels of Malondialdehyde, Antioxidant enzymes and Vitamin E in the serum of women with unexplained infertility and control group. : Case control study. 70 normal ovulatory women who conceived within 12 months of contraceptive free intercourse, and with no history of miscarriage were recruited in the control group. 70 women with unexplained infertility were recruited as study group. All participants included in the study were between 28 and 38 years of age. Serum levels of MDA, Antioxidant enzymes (GST, SOD, Catalase) and Vitamin E concentrations were compared between two groups. Vitamin E concentrations were determined by using High performance liquid chromatography. Antioxidant enzymes and MDA were measured by standard spectrophotometric assay. : Data was analysed using SPSS Software. Continuous parameters were analysed using Mann Whitney U test. There is a significant decrease in the vitamin E levels 3.80 ug/ml) in the unexplained infertile group as compared to controls 6.0 ug/ml). All women in the study group showed significantly (P<0.05) higher levels of MDA and lower levels of antioxidant enzymes such as GST, SOD and Catalase as compared to the fertile women. This decrease was found to be significant with p value of <0.05. The study group have a higher oxidative stress status and low level of antioxidants compared to control group. Serial measurement of oxidative stress biomarkers and their defense system may help to understand the aetiology of unexplained infertility and to enhance their chances of conception.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of Ageratum conyzoides against carbon tetrachloride –induced hepatic damage in rats","authors":"Manik Sharma, Manish Soni, Amita Gupta","doi":"10.18231/j.ijcbr.2023.040","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.040","url":null,"abstract":"The present study was conducted toexamine the Hepatoprotective activity against the carbon tetrachloride toxicity in Wister rats. The Petroleum ether, ethyl acetate and methanol extract of was prepared and evaluated for phytochemical screening. The serum level of glutamic oxaloacetate transaminase (SGOT) Glutomicpyruvate transaminase (SGPT) and bilirubin were investigated for the assessment of hepatoprotective activity of ethyl acetate of extract . Additionally, the histological changes in liver were studied. The primary phytochemical investigation of the extract of revealed the presence of Flavonoids, tannins, and carbohydrates. Pretreatment with ethyl acetate extract of showed a significant (P<0.05) decrease in serum SGOT, SGPT and bilirubin when compared to control group rats treated with CCl in dose dependent manner. The outcomes of histology study revealed that there was significant reversal of histological functions of liver. In conclusion, the findings of study validated that the can improve CCl–induced hepatic toxicity.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diabetes mellitus: A lifestyle disorder","authors":"Abhinav Manish","doi":"10.18231/j.ijcbr.2023.045","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.045","url":null,"abstract":"Diabetes mellitus is a chronic metabolic disorder characterized by elevated blood glucose levels, resulting from either insufficient insulin production or the body's inability to effectively utilize insulin. This condition has become a global health concern, with an increasing prevalence worldwide. This review article aims to provide an overview of diabetes mellitus, its diagnostic criteria, and recent updates in management, complications, and a discussion on its implications for public health.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of serum sodium levels measured using a colorimetric kit approach and those determined using direct and indirect ion selective electrode techniques- in a Hospital Central Lab","authors":"T. Saraladevi","doi":"10.18231/j.ijcbr.2023.027","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.027","url":null,"abstract":"When electrolyte imbalances are not recognized, they are known to cause severe morbidity and mortality. Dyselectrolytemia is frequently curable. Hyponatremia is a common symptom seen in the elderly. Laboratory equipment may be lacking in rural healthcare settings. As a result, the purpose of this study was to investigate the feasibility of developing a screening technique that can aid in the detection of hyponatremia in such facilities. Serum sodium levels in 120 samples were determined by direct, indirect, and colorimetric ISE. SPSS version 17, NCSS 11, and MINITAB 18 software were used for statistical analysis. Statistical significance was defined as a p-value of 0.05. While comparing the instruments, a basic linear regression analysis was performed, and a regression equation for sodium levels was established. The Bland-Altman analysis yielded a 95% agreement limit between the instruments, which is well within the CLIA-recommended target value of 4 mmol/L in the hyponatremic and normonatremic ranges. The resulting regression equation computes a predicted value for direct and indirect ISE using the colorimeter readings, making it similar to all three instruments in the hyponatremic and normonatremic ranges. The colorimetric method can be used as a low-cost screening technique to identify hyponatremic elderly people for whom a tertiary hospital may be inaccessible, allowing for early care.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76833186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of alpha-fetoprotein clinical performances in the diagnosis of the hepatocellular carcinoma at Sominé DOLO hospital of Mopti","authors":"M. Coulibaly, Adama Kondé, D. Traoré, O. Bah, Valentin Sagara, B. Maiga","doi":"10.18231/j.ijcbr.2023.028","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.028","url":null,"abstract":"Serum AFP as a poor clinical performance values especially when it comes to deal with the early and AFP-negative diagnostic of HCC. The aim of this work was to assess the contribution of AFP in the diagnosis of HCC. : A total of 95 subjects was enrolled a prospective observational study by consecutive enrolment and divided in two groups. The first group was made up with subjects in whom the diagnosis of HCC had been retained while the second was the control group which was free of HCC. AFP levels were performed by electrochemiluminescence immunoassay on the cobas e411. Data were captured in Excel and analyzed by Ri386 version 4.1.2 binary for macOS 10.13. The Log of median of AFP in HCC subjects was significantly greater than in non HCC subjects 6.91 ng/mL versus 1.43 ng/mL, Wilcoxon < 0.001. At the cut-off of 200 ng/mL, the clinical performances showed an acceptable sensitivity 97.1% CI 95% [93.7 – 100] but a poor specificity 73,8% CI 95% [64.9 – 82.6] and out of the 34 cases of HCC, one case (2.9%) was AFP-negative HCC. our data show an acceptable sensitivity but a weak specificity of AFP as a biomarker for HCC at cut-off 200 ng/mL. This suggests that AFP should be used with other biomarkers mainly for the early and AFP-negative HCC diagnosis.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72889852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucopolysaccharidosis: An overview and new treatment modalities","authors":"Wilma Delphine Silvia CR, Akshay S Atre, Preksha Shivakumar, A. Chakraborty","doi":"10.18231/j.ijcbr.2023.016","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.016","url":null,"abstract":"Mucopolysaccharidosis is a lysosomal storage disorder, caused due to deficiency of enzymes required for the breakdown of Mucopolysaccharides. These undegraded Mucopolysaccharides accumulate in various tissues and cause characteristic features like neurological deficit, impaired motor function, developmental delay, hearing loss, behavioral problems, corneal clouding, glaucoma, respiratory distress, coarse facial features, skeletal deformities, and organomegaly. Based on deficient enzymes they have divided into subtypes Mucopolysaccharidosis I (MPS I) Hunter syndrome (I H / I HS / I S), Mucopolysaccharidosis II(MPS II) Hunter syndrome (severe and mild form), Mucopolysaccharidosis III (MPS III) Sanfilippo syndrome, Mucopolysaccharidosis IV(MPS IV) Morquio syndrome, Mucopolysaccharidosis VI(MPS VI) Maroteaux Lamy syndrome, Mucopolysaccharidosis VI (MPS VII) Sly syndrome. Diagnosis is classically based on clinical examination and urine analysis. Enzyme assay can also aid in diagnosis. Chorionic villi sampling and amniocentesis are also becoming popular. The main objective of treatment is to improve the quality of life. Symptomatic management includes daily exercise, physiotherapy, tonsillectomy, shunting surgery, and corneal transplantation. There are various recent concepts utilized for the treatment of Mucopolysaccharidosis. This review article emphasizes such treatment aspects as Hematopoietic stem cell therapy, Enzyme replacement therapy, Gene therapy, Nano-enabled therapy, and Substrate reduction therapy.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86614069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between serum alpha-fetoproteinlevel and liver function parameters in hepatocellular carcinoma patients: A case-control study","authors":"Poornima R Varma","doi":"10.18231/j.ijcbr.2023.030","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.030","url":null,"abstract":": Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer occurs in people with liver diseases like chronic hepatitis B and hepatitis C virus. Early diagnosis and monitoring of HCC is possible with the help of tumor marker AFP. The aim of the study was to assess whether AFP level is associated with selected liver function tests in hepatocellular carcinoma patients.: Total 90 individuals are selected, 50 HCC patients with AFP >20ng/ml and 40 subjects (normal LFT and AFP value <10ng/ml) with an age between 40-70. Serum is used for analysis of AFP, SGOT, SGPT, ALP by Maglumi 1000 and Siemen biochemistry analyzer (using principle Chemiluminescence immunoassay) respectively. Unpaired T test (IBM SPSS 22.0) were used. Elevated levels of AFP (p value <0.05), SGOT (p value < 0.001), SGPT (p value < 0.001), ALP (p value < 0.001) shows statistical significance within study population and also indicated the risk of developing HCC with respect to AFP level in liver disease patients with elevated LFT parameters.: From the study, it is concluded that LFT and AFP are comparatively elevated in HCC. Treatment is usually not possible with advanced HCC due to failure of not doing proper screening at appropriate time. Early diagnosis and monitoring of HCC are possible with the help of tumor marker AFP.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77995154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of anemia on HbAC level in type 2 diabetes mellitus patients","authors":"G. Gulavani, S. Panchbudhe, S. Gawade, Farheen B Mujawar","doi":"10.18231/j.ijcbr.2023.020","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.020","url":null,"abstract":"HbA1c is one of the important laboratory markers of blood glucose levels monitoring over last 2-3 months. Studies have shown that many factors affect HbA1c levels, hence it is not the only investigation to diagnose diabetes mellitus. As both iron deficiency anaemia and diabetes mellitus are highly prevalent disorders, effect of iron deficiency anaemia on HbA1C need to be evaluated. Many factors such as haemolytic anaemia, vitamin deficiencies, pregnancy. Kidney disease, haemoglobin variants can affect HbA1C levels. This study had two groups of participants. Group 1 included diabetic patients with iron deficiency anaemia and group 2 included diabetic patients without iron deficiency anaemia. Significant correlation between HbA1C and iron deficiency anaemia has been found. PCV, MCH, MCHC, MCV are low in diabetics with Iron deficiency anaemia and high in diabetics without iron deficiency anaemia. Need for anaemia screening before treatment planning of diabetes based on HBA1C levels must be considered and for optimal diabetes control, treating anemia is necessary.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86083596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of analytical performance & quality specification of urine biochemical analytes","authors":"Ashwini Pandith","doi":"10.18231/j.ijcbr.2023.017","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.017","url":null,"abstract":"Urinary biochemical analytes are very important tools for clinical decision making. Total allowable error (TEa) by integrating internal (IQC) and external (EQC) quality control performances are used to evaluate the performance of urinary biochemical analytes along with quality specifications strategy. : Alternate 6 months Coefficient of Variation (CV%) and External Quality Assurance Scheme (EQAS) bias% data for urinary biochemistry analytes were collected for the year 2022. TEa calculated for each analyte was calculated based on average CV% and bias%. Total TEa calculated values are compared with optimal, minimal and desirable TEa of each analyte. : TEa values of urinary biochemistry analytes were performing good and fulfilled minimal, desirable and optimal quality requirements except urine creatinine which did not fulfill the minimal standards.: TEa is an excellent quality management tool and quantitatively evaluates analytical performance. The accurate results generated are useful for clinicians for decision-making.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79602386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A research on the chemical constituent, characterization and remedial benefits of a native medicinal herb","authors":"Srashti Choudhary, Diksha Panwar, Aditi Tyagi, Kaveri Bisht, Neetu Sharma, B. Bisht, Promila Sharma","doi":"10.18231/j.ijcbr.2023.026","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.026","url":null,"abstract":"Medicinal plants have been used for thousands of years by people around the globe. From ancient times to the end of the nineteenth century, herbs have proven to be the major source of remedial therapies and treatments. Evidence has been collected for ages to reveal the good perspective of herbal plants utilized in numerous supportive, traditional, and different systems, with the ever-enlarging scrutiny of the present-day population with regard to legitimate results. is a predominant medicinal herb that is widely utilized for the cure of many ailments. The sample of was selected from the Uttarkashi Garhwal region of Uttarakhand, and then further identification was done by a designated authority from Dehradun, i.e., the Forest Research Institute (FRI), and steam distillation of fresh leaves was performed and oil extract was collected. The yield of essential oil from leaves was observed and a total of fifty constituents were recognized with the help of Gas Chromatography-Mass Spectrometry (GC-MS). The crucial oil also has antifungal and antibacterial properties against some infectious strains. The engrossing characteristics of the oil constitution were the existence of dictamnol in good amounts. In the current research assignment, plant samples were gathered from the hilly regions of Uttarkashi for oil extraction and identification of chemical constituents.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89732625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}