不明原因不孕妇女维生素E水平、抗氧化酶和丙二醛的评价

None Vanitha S, Victoria Job, None Aleyamma T K
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摘要

不明原因不孕是一种令人困惑的疾病。30%的不孕夫妇被诊断为不明原因的不孕症,所有标准生育调查正常。氧化应激已被证明对患有子宫内膜异位症和多囊卵巢综合征的妇女的生殖功能有负面影响。在另一项研究中,补充抗氧化剂可改善OS诱导的不孕症。许多研究表明,不孕症妇女血清中丙二醛含量增加,维生素E作为体内保护性抗氧化剂对生育能力有积极作用。越来越多的证据表明,OS与不明原因的不孕症有关。基于这些知识,我们选择了一组特殊的原因不明的不孕妇女来评估她们的血清抗氧化酶、维生素E和脂质过氧化标志物的水平。目的比较不明原因不孕妇女与对照组血清丙二醛、抗氧化酶和维生素E水平。病例对照研究。70名正常排卵的妇女,在12个月内怀孕,没有流产史,被招募为对照组。选取70例不明原因不孕妇女作为研究对象。所有参与研究的人的年龄都在28到38岁之间。比较两组大鼠血清MDA、抗氧化酶(GST、SOD、过氧化氢酶)水平和维生素E浓度。采用高效液相色谱法测定维生素E浓度。用标准分光光度法测定抗氧化酶和丙二醛。:数据分析采用SPSS软件。采用Mann Whitney U检验对连续参数进行分析。不明原因不育组的维生素E水平(3.80微克/毫升)明显低于对照组(6.0微克/毫升)。与育龄妇女相比,研究组的所有妇女都显示出显著(P<0.05)较高的MDA水平和较低的抗氧化酶水平,如GST、SOD和过氧化氢酶。p值为<0.05时,这种下降是显著的。与对照组相比,研究组的氧化应激状态较高,抗氧化剂水平较低。氧化应激生物标志物及其防御系统的连续测量可能有助于了解不明原因不孕症的病因,并提高其受孕机会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of vitamin E levels, antioxidant enzymes and malondialdehyde in women with unexplained infertility
Unexplained infertility is a perplexing disorder. 30% of infertile couples are diagnosed with unexplained infertility with all standard fertility investigations normal. Oxidative stress has been shown to have a negative impact on reproductive function in women with endometriosis and (PCOS) polycystic ovarian syndrome. In another study antioxidant supplementation improved OS induced infertility. Many studies have demonstrated an increase in Malondialdehyde in serum of women with infertility and vitamin E as a protective antioxidant in the body with positive effect on the fertility. There is growing evidence linking OS and unexplained infertility. Based on this knowledge the specific group of unexplained infertile women were chosen to assess their levels of serum antioxidant enzymes, Vitamin E and lipid peroxidation marker. To compare the Levels of Malondialdehyde, Antioxidant enzymes and Vitamin E in the serum of women with unexplained infertility and control group. : Case control study. 70 normal ovulatory women who conceived within 12 months of contraceptive free intercourse, and with no history of miscarriage were recruited in the control group. 70 women with unexplained infertility were recruited as study group. All participants included in the study were between 28 and 38 years of age. Serum levels of MDA, Antioxidant enzymes (GST, SOD, Catalase) and Vitamin E concentrations were compared between two groups. Vitamin E concentrations were determined by using High performance liquid chromatography. Antioxidant enzymes and MDA were measured by standard spectrophotometric assay. : Data was analysed using SPSS Software. Continuous parameters were analysed using Mann Whitney U test. There is a significant decrease in the vitamin E levels 3.80 ug/ml) in the unexplained infertile group as compared to controls 6.0 ug/ml). All women in the study group showed significantly (P&#60;0.05) higher levels of MDA and lower levels of antioxidant enzymes such as GST, SOD and Catalase as compared to the fertile women. This decrease was found to be significant with p value of &#60;0.05. The study group have a higher oxidative stress status and low level of antioxidants compared to control group. Serial measurement of oxidative stress biomarkers and their defense system may help to understand the aetiology of unexplained infertility and to enhance their chances of conception.
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