International Journal of Clinical Biochemistry and Research最新文献

{"title":"A review on biomarkers of hypertension","authors":"Vijatha Thomas, Arun T. Mithrason","doi":"10.18231/j.ijcbr.2022.037","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.037","url":null,"abstract":"Hypertension is the most prevalent chronic medical condition seen in primary care. It is a silent disease because of its asymptomatic nature and is usually diagnosed at advanced stage. It effects various organs like heart, kidney, etc. It is responsible for 10.8% deaths in India. Over the years many biomarkers are identified to understand the pathophysiology of hypertension. Biomarkers have unique role in prognosis as the level rises before the onset of overt hypertension. Clinically it is beneficial as it helps in identifying the high risk patients for better treatment and prognosis. In this review we have highlighted the importance of various biomarkers of hypertension in early diagnosis, before the onset of overt hypertension, which is associated with long-term end-organ diseases.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90040526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and biochemical parameters of smokers and alcohol consuming adults from Terai region of Nepal using cross-sectional study","authors":"Birendra Kumar Jha, M. Sherpa, J. Singh, B. Dahal, Chamma Gupta","doi":"10.18231/j.ijcbr.2022.044","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.044","url":null,"abstract":"Non-communicable chronic diseases (NCD) are contributing 46% to the global diseases burden and account for 59% of annual death. Tobacco and alcohol consumptions are reported as major factors associated with growing burden of NCDs and account for 11.5% of global death with around 80% of these deaths are reported in low middle-income countries.Nepal, a low middle-income county, is also facing an increasing prevalence (31%) of NCDs with a significant prevalence of smoking (37.1%) and alcohol consumption (15%-57%). To explore the physiological and biochemical parameters of smoker and alcohol consuming subjects of Terai region of Nepal. We conducted a community based cross-sectional study during August to November 2019, 282 adult participants were selected from Terai region of Janakpur Zone, Nepal, After obtaining written consent and clinical examination, fasting venous blood was collected from study subjects and examined for triglycerides, total cholesterol, HDL-cholesterol, LDL-cholesterol and fasting blood sugar.We observed significantly increased triglycerides (p<0.05), and waist circumference (p<0.05) in current smoking and alcohol consuming subjects, however diastolic blood pressure was found to be significantly higher among alcohol consuming subjects only (p=0.05). We did not observe any significant correlation between fasting blood sugar, total-cholesterol and LDL-cholesterol among smoking or alcohol consuming subjects.Significantly increased triglycerides, waist circumference and diastolic blood pressure in smoking and alcohol consuming subjects likely suggest lack of public awareness and heath promotion activities in Teari region of Nepal and indicate an urgent public health awareness programs in this region to maintain quality of life.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86960553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in-vitro analysis of L-Carnitine mediated rescue of TNF-? induced apoptosis in mice oocytes","authors":"Mona Sharma, Ranjana Rana, Ashutosh Halder, Surabhi Gupta","doi":"10.18231/j.ijcbr.2022.043","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.043","url":null,"abstract":": Tumor necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine implicated in various physiological and pathological events. Carnitine is a quaternary amine which plays a significant role in fatty acid oxidation and is reported to produce antiapoptotic effects. Aim of this work was to study the effect of L-Carnitine (LC) on TNF-α induced apoptosis in mice oocytes. : Oocytes were isolated from super ovulated Swiss Albino mice and treated with different concentrations of TNF-α (0.1ng/ml, 1ng/ml, 10ng/ml, 100ng/ml) and LC (0.1mg/ml, 0.3mg/ml, 0.5mg/ml, 1.0 mg/ml). TUNEL Assay was done for the biochemical assessment of apoptosis.: Apoptotic indices with different doses of TNF-α (0.1ng/ml, 1ng/ml, 10ng/ml, 100 ng/ml) were 28.5%, 71.4%, 100%, 42.8% respectively. The concentration of TNF-α that produced the highest apoptotic index was 10ng/ml. LC alone in different doses (0.1mg/ml, 0.3mg/ml, 0.5mg/ml, 1.0 mg/ml) did not elicit any apoptotic signal. Further LC was added in different doses with 10ng/ml TNF-α to study the rate of apoptosis in mice oocytes. Apoptotic index with 10 ng/ml TNF-α and different doses of LC (0.1mg/ml, 0.3mg/ml, 0.5mg/ml, 1 mg/ml) were 25%, 37.5%, 50%, 62.5% respectively. The concentration of LC that reduced the apoptotic index to the maximum was 0.1mg/ml.: Present study could demonstrate the anti-apoptotic effect of LC against apoptotic effects of TNF-α in mice oocytes. The study presents preliminary data suggesting a possible therapeutic role of LC in inflammatory etiologies such as ovarian failure.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"1 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90789407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To find the impact on learning among phase 1 MBBS students during COVID-19 pandemic","authors":"Vijatha Thomas, Arun T. Mithrason","doi":"10.18231/j.ijcbr.2022.046","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.046","url":null,"abstract":"The closure of educational activities around the world due to the ongoing COVID-19 pandemic resulted in an unplanned shift from traditional learning to a setup that exclusively involves digital teaching and learning. Within this context, the present study aimed to explore undergraduate medical students’ perceptions regarding the effectiveness of synchronized online learning at DM WIMS, Wayanad, Kerala. : A cross sectional study was done in DM WIMS, Wayanad, Kerala and included 120 first MBBS students. First a five point Likert scale questionnaire specific to their level of training was sent online via Google form. Next, 3 focus group were conducted on 3 different days. Number of students in each group were 8. Duration of each focus group discussion was 1 hr. Focus group discussion was done in the department of Biochemistry. All interviews were recorded, transcribed and analyzed for thematic contents. : Various challenges to learning as agreed upon by majority of students included not familiar with Google meet (80%), could not concentrate for long during sessions (75.8%), less interaction, not able to clarify doubts, network and connectivity issues (70%) and not adequate feedback given during the sessions (59.1%). Various benefits to learning during COVID -19 pandemic as agreed by students included: online ppt shared via Whatsapp were useful (86.6%), better time management (65.9%), and use of videos and pictures during sessions enhanced understanding the concept better (41.5%). A thematic analysis yielded three core themes: (1) Overall learning experience, (2) Quality of teaching and learning, (3) Difficulties encountered. Majority of participants said it was good learning experience and new for them. They said they were reluctant and anxious about going online initially but eventually they picked up and were satisfied with most of the sessions. Majority of students were satisfied with the teaching and agreed that the lectures were well taken.: It is true to say that all facets of health education and clinical training is harshly affected by the pandemic. Despite all these barriers and challenges, it has been demonstrated that online learning holds certain advantages over traditional learning.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75562362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of copper and zinc in liver diseases","authors":"R. Nehra, Dwijendar Nath, A. Nasir, Aamir Hasan, Narottam Aggarwal, M. Nehra","doi":"10.18231/j.ijcbr.2022.038","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.038","url":null,"abstract":"The liver is a primary storage site for a variety of metabolites. It plays a role in wide variety of metabolic, toxic, excretory, circulatory and defence functions inside the body. Damage to the organ may not evidently affect its activity since the liver has considerable functional reserve. Liver disease is a common term for any damage that reduces the functioning of the liver. Chronic liver disease is identified by gradual destruction of liver cells resulting in fibrosis. It is affected by different conditions including viral hepatitis, excessive alcoholism, genetic, autoimmune and NAFLD. The present study was carried out in Govt. Medical College, Jalaun at Department of Biochemistry. The diagnosis of Liver disease was done by ultrasonographic examination of liver. This study comprised a total of 50 patients, 25 of whom were healthy controls and 25 of whom were Liver Disease patients. After overnight fasting 8-12 hours (under aseptic condition) blood sample (8ml) was drawn from antecubital vein of each subject using a plain vial and was analyzed for serum Cu, Zn & liver profile parameters (serum bilirubin, serum SGOT, serum SGPT and serum ALP done by colorimetric method and calmagnite method, diazo method, IFCC method and assessed by kinetic method respectively. The present study’s findings, indicates that the level of serum copper in liver disease patient was 127.38±28.81µg/dl, which is significantly higher than that of 86.54 ± 15.88µg/dl found is healthy controls (p<0.001). Also, serum copper is strongly linked with biochemical parameters of liver enzyme (SGOT & SGPT). Likewise, the mean value of serum zinc in liver disease were 58.08 ± 13.11µg/dl, which is significantly lower than that of69.88± 6.67µg/dl found in healthy subjects. Further, serum zinc has shown a significant weakly linked with serum SGPT.Therefore, during routine assessments of individuals with liver disease, serum trace elements (Cu and Zn) can be highly effective indicators for detecting the severity of liver damage. Thus, based on the findings of our current investigation, zinc and copper supplementation, as well as a reduction in copper intake, may help to enhance patient survival and preventing the development of hepatitis B to liver cirrhosis.Considering findings of the study, it is recommended that serum trace element concentrations be corrected on a regular basis to help with various problems of liver cirrhosis and maybe to slow the progression of liver disease.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"145 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86215471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of serum ferritin and thyroid stimulating hormone (TSH) levels in hypothyroid and euthyroid subjects","authors":"Sumitha Prabhu P S, Sumina Cheriyan, Libna Sulaiman","doi":"10.18231/j.ijcbr.2022.048","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.048","url":null,"abstract":"Thyroid hormones synthesized and released by the thyroid gland, have a vital role in regulating the metabolism of body. Synthesis of these thyroid hormones requires an iron containing enzyme Thyroid Peroxidase (TPO). Thus, iron inadequacy can affect the proper functioning of the TPO enzyme that further affect the thyroid hormone production. Serum ferritin, an index of iron store is present in almost all cells; however, it has been reported that an alteration in ferritin levels occurs in patients with thyroid disease. This study was conducted to determine serum ferritin and Thyroid Stimulating Hormone (TSH) levels in hypothyroid and euthyroid subjects and to compare the same.The retrospective study was carried out in Amrita Institute of Medical Sciences (AIMS), Kochi, which included 30 hypothyroid subjects as cases and 30 age and gender matched healthy controls. TSH, Thyroxine (T4) and serum ferritin levels of the study groups were collected and recorded.The mean TSH of hypothyroid patients was found to be higher (16.12±17.00) than that of euthyroid group (2.35± 1.08) whereas the mean value of T4 (1.01± 0.25) and ferritin (43.80 ± 75.44) were found to be reduced in patients with hypothyroidism compared to normal subjects. All the parameters were statistically significant with p value <0.05.The present study showed that hypothyroid subjects had significantly lower serum ferritin concentration than euthyroid subjects. Thus, serum ferritin measurement could be useful for the evaluation of thyroid diseases.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90094957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of sodium fluoride and serum separator tubes for blood glucose estimation – What’s the best option?","authors":"Joel Kevin Raj Samuel, S. Chandrasekaran","doi":"10.18231/j.ijcbr.2022.041","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.041","url":null,"abstract":"The efficacy of sodium fluoride tubes in preserving glucose was compared against a serum separator tube with a gel barrier. We compared the fall in blood glucose levels in a fluoride - oxalate tube against levels in a serum separator tube. A heparinized tube was used as a standard.45 samples were drawn from healthy patient volunteers and distributed into the different tubes. Patients on medications or with chronic kidney disease were excluded. Glucose estimation was done by glucose oxidase-peroxidase method using a semi-automated colorimetric analyser. Samples were immediately centrifuged and stored at room temperature. The fall in glucose levels in each tube was measured at predefined time intervals of 1, 2, 4, 12 and 24 hours.There was no statistically significant difference in the glucose values measured in the fluoride and serum separator tubes at all time intervals from 1 to 24 hours. There was no statistically significant difference in the glucose values measured in all three tubes until 4 hours. However, there was an error beyond acceptable limits (for medical decision making as per CLIA criteria) in the difference in values between the fluoride and heparin tubes at 2 and 4 hours.Thus, there is no difference between use of a fluoride tube or a serum separator tube for up to 24 hours. A serum separator tube can be used when many analytes are to be measured in the same sample and a fluoride tube can be used when only glucose is to be estimated. A heparinized tube will suffice if glucose estimation will be done within one hour of collection.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75096389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activities of sweet orange (Citrus sinensis) juice on isolated Staphylococcus aureus, Escherichia coli, Pseudomonas spp, and Klebsiella spp","authors":"E. C. Ogbodo, J. Iwuji, S. Chukwuemeka, E. Ogbonye, A. Amah, I. Uduchi, A. Okezie, A. A. Okebalama","doi":"10.18231/j.ijcbr.2022.047","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.047","url":null,"abstract":"Resistance to antibiotics is one of the greatest problems to the success of modern medication. Due to the emergence of multi-drug resistant bacteria, selection of effective antibiotics for suppression of microbial activity has been an incidental issue. The aim of this study is to determine the antimicrobial activities of sweet orange juice on isolated, , which were subjected to different concentrations of sweet orange juice to determine their response to the juice concentrations. 25%, 50%, & 100% concentrations were used respectively using a well-in-agar diffusion method and it was discovered that all the microorganisms were sensitive to the sweet orange juice at 100% concentration only with zones of inhibitions as follows: This shows that the juice may not be recommended as alternative for antibiotics development since it only inhibited the microbial growth at 100% concentration only, and its respective zones of inhibition were minimal or less effective.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82231066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of climate change and human life","authors":"Ravichandran","doi":"10.18231/j.ijcbr.2022.036","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.036","url":null,"abstract":"","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77016276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trace elements in hypothyroidism in relation to L-thyroxine therapy","authors":"S. Mahmood, Tamkinath Fatima, S. Anees","doi":"10.18231/j.ijcbr.2022.050","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.050","url":null,"abstract":"Hypothyroidism is a common disorder in the population with a female predominance. Most hypothyroidism cases were found in the age group range of 30–35 in both the sexes of individuals but women were more prone to develop it. Disturbance or imbalance in the thyroid hormone levels is the underlying cause of resultant hypothyroidism. A comparative study of serum T3 (triiodothyronine), T4 (thyroxine), TSH (thyroid stimulating hormone), copper, zinc, magnesium and iron was done in 100 normal individuals as controls (Group I). 100 Hypothyroid patients showing Good Response to Levothyroxine Therapy – GRLT as (Group II A) and 100 Hypothyroid patients showing Poor Response to Levothyroxine Therapy – PRLT as (Group II B) over a period of 1 and half year in the age group of 20 to 45 years. The levels of TSH were found to be elevated in hypothyroid patients with relatively lower levels of serum T3 and T4 as per our study conducted in hypothyroid patients with PRLT when compared with the healthy subjects and patients in GRLT group. A study of implementation of levothyroxine therapy in hypothyroid subjects showed improvement in the patients with all the hormone levels returning back to the pre – hypothyroid state in majority of the hypothyroid patients with GRLT in comparison with the controls. Results of the study indicate a significant decreased levels of serum copper, zinc and iron in hypothyroid patients showing poor response to levothyroxine therapy whereas the magnesium levels in the serum of hypothyroid individuals with poor response to levothyroxine was found elevated. The serum levels of all the trace elements studied i.e., copper, zinc, magnesium, and iron in GRLT hypothyroid individuals and controls were within the normal ranges. Further studies are however needed on these parameters for establishing serum levels of trace elements in hypothyroidism in relation to response to levothyroxine therapy.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84850384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}